MELO3C012350.2 (gene) Melon (DHL92) v3.6.1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	MELO3C012350.2
Type	gene
Organism	Cucumis melo (Melon (DHL92) v3.6.1)
Description	iron-sulfur assembly protein IscA-like 1, mitochondrial
Location	chr10 : 1096823 .. 1099717 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AACAAAATTACGACGAATTGTAATTCAAGCAAATGCGTATATCGGAAAACACTCGAAAGTTGTAGTTTGCGGAGGAGAAAATTGATCGATAGGCAAAAAGAAGTGAGAAAATGGCAGCTTCTTCGTCTTCCTTTTTCGCCGCCGCTTCTCAGAAGGCCGGACCAGCCGTTCGCCGCCAAATTCTTACTCTCACGGAGGCAGCCGCCGCTAGAATTCGACAACTTCTTCAACAGCGCCAACGTCCATTCCTTCGTCTTGGCGTCAAGGCGCGCGGTTGTAATGGTTTATCCTATACTCTCAACTACGCAGGTCTGTAATAGCCTAATGAATTTCAACTTTTTATTGATTCTACGTAATTTCTCTTTTACCCATGTGATTGAATTTGTCGATTTTCTATCTCTTTTCTTTGAATCAGATTTTGATTTTGGATTTTATTGGAGATAAATAGGTATTTGGTGTATGTGAGCGATACAACCTAATAGTTTAGAGGAGATGTTTCGGAACTAGTCTTTTGGAAGATTTTTTTAAAAAATATTTGTACTGTTTCCAGCTCCAATTACCAAAATTAGGATTTTAAGACCGATGATCTATTATGATGATTAGCCGTAAAAAATTAAGAAGGTCAAATGATTCTGTAATTATTATAACCGGAATTGGGCATAGGTTGGTATGTAAGTTGTTCGGAGATGAGCTGCTGAGTTCTTACTAGCCGTAACAACGGCAAATCTGAACCATAAAACTTGACGTTCCTGAATGCATAAGTTGATAAAAAATCGATGATTTTACATACAGGGCCAGGGACCTATGTATGAGAGGAGAATTGACTTAACTACTTCAGTCTGTAAATTATTCTGTATCTTATAGAATCAGTTATGAATTAATGGTGCTATGGGACAGAGAGTTTGGATTCGATGATTTCCTTATCCAACCTATGGCACAGGGATTATTGTGTTATTGATATATCAGAATGTCAAACTAAATGCACGGTTCACCAAAATTTATGGTGAACATTTGCTTCCATCAAGTATCATTTAGATTGTGGTGTATGTTGGCTATTATGAGAACATAGCAGGATTGGGTATCGATTAAATTGACTTGAGAATCTTTGTCTCTCCCTCCTGCTATGGTTAATTATAATGTTATTAGACCAAAGTGCTATTTCTGTTCATTTAGAAGATAAATGGGTTGGCAATCCTCCATGGGTCAGCTGGAAGAGAATAGTTATTAACATGTTTGATGGTTGAAATTTGAGATTTTATACTCTTTGTGGCTTGGGATTTTTTATTGGTTGTGCTTATTTAATATGACTATCTTCTCTGTTAACAGTATTTATAATTTAGTTTTTTATGGAGTTGCTCATTATTGGAGAGTTATCTAACTTGGTATTACCTCAGTTTTTTTTCTCACATTATCGTTAACATTTTCTTTATGCAAGAAATAGGATTCATCCTTTATCTATGTTTGGCATAAATTGACTATAGTTACGATCTCTATCCTTTGAAATACTTTAAGATTGCAGTATTTCATTTAACAGTTTCCTTTTTCAAATATTTCAGATGAGAAAGGGAAGTTTGATGAGCTGGTTGAGGATCAAAATGTGAAAATACTGATCGATCCAAAGGCTCTAATGCACGTAATAGGAACAAAAATGGACTTTGTCGATGACAAATTAAGGTATTTGATAAATCTCTTTCCTAGAATTTTATTTATTCAGCTCTATATATCCATCCTTAAGTTGATGAACTATCCTGCTTGTTAATGACAACATCAGGCTGAAATGTAAAAGTTTGTGTACTTCTTATATTGAAACGTCCACAAGAGTTAGTGATGTAATTGCTCTAGTTAGGATTTATTGTGGGTTTGTATTAAGTCTATCATAGATACAAATTTCAAGGTTAGTTCTCAAGGTTAATTAATTCTTTTAATTATAATTAACTGGCTACCGGTTTTATCTTTCACGTGTAATCATTTTAGATCGTTGGAGTTGAGCTTATATATAAAGCTCCTCCATCTTCTTCAGTTTGGAGGTAATAATATCACAGGGGTTATTTCTTCAAGAAAATTCTCCAAGGTTTTGGATTAAATCACTTTGCTTTAGAGTGGATTTAGATCATCTTCAACACATCCACAAGCTGGTGAAAATAATTAATACAGCTTAAGAGAGAACTGTTAAGTTTATTTATTAGATTCAATTCATCTTAGTATAGCTATGTGGATAAGACACTCGGTACTAACTTAAAAGGATGATGGTTTGATCCTCTACCTTGCAATGGATGGACAAAAAAAAGTTCATTAGATTATGGTTATTTTGAGTCTCTATGTCAAATCAAATTCAAATTCAATTTTTTAACCTCTTGAACCACTATATTCAGTGGCCATTGAAACTTATCTTTTTTCTCTCTTTTCTCTTGTTGGTAGATCTGAGTTTATCTTTATCAATCCAAATTCCAAAGGTCAATGTGGCTGTGGGGAGTCCTTTATGACAACAAGTAGTGCAGAAGCCAACAAAAAATGAGGCAGTTCGAGCAATGAGCGTTGTTTTTAAGAACTCAAAACTCACAGTTGCTGATTTGTTGTGTATTTTGCTGGAATTACAAGCACAATGATCAAGAGAGTTCTCTGACAACAACTTTAAACATTTGTAAGAATATTTCTATATTGGCTGATTATATTCTTAGAAAGCCAGTTAAGAGTTGTACTCTGCTAATTTTTTTTCTTTAAAAATATTTTGTATGTATATTACTGTAGATTAAACTGGCTAATGAGTTATATATGAGAGCCTATAAGGTAATAAAATTCGCCCACGACTTTCTTTTTGTTAAAGTCATAGAACTTGGTAACTCGAGCATCTTGCTTGCCTTATCACTGGTTGAGTAATGAGAATGGTTTGTGTCC

mRNA sequence

AACAAAATTACGACGAATTGTAATTCAAGCAAATGCGTATATCGGAAAACACTCGAAAGTTGTAGTTTGCGGAGGAGAAAATTGATCGATAGGCAAAAAGAAGTGAGAAAATGGCAGCTTCTTCGTCTTCCTTTTTCGCCGCCGCTTCTCAGAAGGCCGGACCAGCCGTTCGCCGCCAAATTCTTACTCTCACGGAGGCAGCCGCCGCTAGAATTCGACAACTTCTTCAACAGCGCCAACGTCCATTCCTTCGTCTTGGCGTCAAGGCGCGCGGTTGTAATGGTTTATCCTATACTCTCAACTACGCAGATGAGAAAGGGAAGTTTGATGAGCTGGTTGAGGATCAAAATGTGAAAATACTGATCGATCCAAAGGCTCTAATGCACGTAATAGGAACAAAAATGGACTTTGTCGATGACAAATTAAGATCTGAGTTTATCTTTATCAATCCAAATTCCAAAGGTCAATGTGGCTGTGGGGAGTCCTTTATGACAACAAGTAGTGCAGAAGCCAACAAAAAATGAGGCAGTTCGAGCAATGAGCGTTGTTTTTAAGAACTCAAAACTCACAGTTGCTGATTTGTTGTGTATTTTGCTGGAATTACAAGCACAATGATCAAGAGAGTTCTCTGACAACAACTTTAAACATTTGTAAGAATATTTCTATATTGGCTGATTATATTCTTAGAAAGCCAGTTAAGAGTTGTACTCTGCTAATTTTTTTTCTTTAAAAATATTTTGTATGTATATTACTGTAGATTAAACTGGCTAATGAGTTATATATGAGAGCCTATAAGGTAATAAAATTCGCCCACGACTTTCTTTTTGTTAAAGTCATAGAACTTGGTAACTCGAGCATCTTGCTTGCCTTATCACTGGTTGAGTAATGAGAATGGTTTGTGTCC

Coding sequence (CDS)

ATGGCAGCTTCTTCGTCTTCCTTTTTCGCCGCCGCTTCTCAGAAGGCCGGACCAGCCGTTCGCCGCCAAATTCTTACTCTCACGGAGGCAGCCGCCGCTAGAATTCGACAACTTCTTCAACAGCGCCAACGTCCATTCCTTCGTCTTGGCGTCAAGGCGCGCGGTTGTAATGGTTTATCCTATACTCTCAACTACGCAGATGAGAAAGGGAAGTTTGATGAGCTGGTTGAGGATCAAAATGTGAAAATACTGATCGATCCAAAGGCTCTAATGCACGTAATAGGAACAAAAATGGACTTTGTCGATGACAAATTAAGATCTGAGTTTATCTTTATCAATCCAAATTCCAAAGGTCAATGTGGCTGTGGGGAGTCCTTTATGACAACAAGTAGTGCAGAAGCCAACAAAAAATGA

Protein sequence

MAASSSSFFAAASQKAGPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTSSAEANKK

Homology

[Close]

BLAST of MELO3C012350.2 vs. NCBI nr
Match: XP_008446757.1 (PREDICTED: iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucumis melo])

HSP 1 Score: 241.5 bits (615), Expect = 1.7e-60
Identity = 121/121 (100.00%), Postives = 121/121 (100.00%), Query Frame = 0


Query: 17  GPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELV 76
           GPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELV
Sbjct: 17  GPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELV 76

Query: 77  EDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTSSAEANK 136
           EDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTSSAEANK
Sbjct: 77  EDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTSSAEANK 136

Query: 137 K 138
           K
Sbjct: 137 K 137

[Close]

BLAST of MELO3C012350.2 vs. NCBI nr
Match: XP_022956980.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita moschata])

HSP 1 Score: 235.3 bits (599), Expect = 1.2e-58
Identity = 117/121 (96.69%), Postives = 119/121 (98.35%), Query Frame = 0


Query: 17  GPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELV 76
           GPA RR+ILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELV
Sbjct: 17  GPAARRKILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELV 76

Query: 77  EDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTSSAEANK 136
           EDQNVKILI+PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTT SAEANK
Sbjct: 77  EDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTGSAEANK 136

Query: 137 K 138
           K
Sbjct: 137 K 137

[Close]

BLAST of MELO3C012350.2 vs. NCBI nr
Match: XP_023539778.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 234.6 bits (597), Expect = 2.0e-58
Identity = 117/121 (96.69%), Postives = 118/121 (97.52%), Query Frame = 0


Query: 17  GPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELV 76
           GPA RRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELV
Sbjct: 17  GPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELV 76

Query: 77  EDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTSSAEANK 136
           EDQNVKILI+PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTT SAEA K
Sbjct: 77  EDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTGSAEAKK 136

Query: 137 K 138
           K
Sbjct: 137 K 137

[Close]

BLAST of MELO3C012350.2 vs. NCBI nr
Match: PON84141.1 (FeS cluster insertion protein [Trema orientalis])

HSP 1 Score: 224.2 bits (570), Expect = 2.8e-55
Identity = 109/121 (90.08%), Postives = 118/121 (97.52%), Query Frame = 0


Query: 17  GPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELV 76
           GPA+RRQ+L+LT+AAA RIRQLLQQRQR FLRLGVKARGCNGLSYTLNYADEKGKFDELV
Sbjct: 14  GPALRRQVLSLTDAAADRIRQLLQQRQRSFLRLGVKARGCNGLSYTLNYADEKGKFDELV 73

Query: 77  EDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTSSAEANK 136
           ED+ VKILIDPKALMHV+GTKMDFVDDKLRSEFIF+NPNSKGQCGCGESFMTTSSAEA+K
Sbjct: 74  EDKGVKILIDPKALMHVVGTKMDFVDDKLRSEFIFVNPNSKGQCGCGESFMTTSSAEASK 133

Query: 137 K 138
           +
Sbjct: 134 R 134

[Close]

BLAST of MELO3C012350.2 vs. NCBI nr
Match: XP_022968348.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita maxima] >XP_022968350.1 iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita maxima] >XP_022968351.1 iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita maxima] >XP_022968352.1 iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita maxima] >XP_022968353.1 iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita maxima])

HSP 1 Score: 223.0 bits (567), Expect = 6.2e-55
Identity = 110/118 (93.22%), Postives = 115/118 (97.46%), Query Frame = 0


Query: 17  GPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELV 76
           GPA RRQILTLTEAAAARIRQLLQQRQR FLRLGVK+RGCNGLSYTLNYADEKGKFDELV
Sbjct: 17  GPAARRQILTLTEAAAARIRQLLQQRQRSFLRLGVKSRGCNGLSYTLNYADEKGKFDELV 76

Query: 77  EDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTSSAEA 135
           ED++VKIL+DPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTT SA+A
Sbjct: 77  EDKDVKILVDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTGSAKA 134

[Close]

BLAST of MELO3C012350.2 vs. TAIR10
Match: AT2G16710.3 (Iron-sulphur cluster biosynthesis family protein)

HSP 1 Score: 202.2 bits (513), Expect = 2.0e-52
Identity = 97/126 (76.98%), Postives = 114/126 (90.48%), Query Frame = 0


Query: 17  GPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYA------DEKG 76
           GPA+R+Q+LTLT+ AA+R+  LLQQRQ+PFLRLGVKARGCNGLSYTLNYA      DEKG
Sbjct: 15  GPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLNYAGKTSGNDEKG 74

Query: 77  KFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTS 136
           KFDELVE++ V+IL++PKALMHVIGTKMDFVDDKLRSEF+FINPNS+GQCGCGESFMTTS
Sbjct: 75  KFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVFINPNSQGQCGCGESFMTTS 134

[Close]

BLAST of MELO3C012350.2 vs. TAIR10
Match: AT2G36260.1 (Iron-sulphur cluster biosynthesis family protein)

HSP 1 Score: 174.5 bits (441), Expect = 4.6e-44
Identity = 81/110 (73.64%), Postives = 100/110 (90.91%), Query Frame = 0


Query: 20  VRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQ 79
           +R+Q+L L++ AAARIRQLLQ +Q+PFLRL V+A+GCNGLSY LNYA EKGKFDE+VE++
Sbjct: 1   MRKQVLALSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYAQEKGKFDEVVEEK 60

Query: 80  NVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTT 130
            VKIL+DPKA+MHVIGT+MDFVDDKLRSEF+F+NPN+  +CGCGESF TT
Sbjct: 61  GVKILVDPKAVMHVIGTEMDFVDDKLRSEFVFVNPNAT-KCGCGESFTTT 109

[Close]

BLAST of MELO3C012350.2 vs. TAIR10
Match: AT1G10500.1 (chloroplast-localized ISCA-like protein)

HSP 1 Score: 73.2 bits (178), Expect = 1.4e-13
Identity = 36/105 (34.29%), Postives = 62/105 (59.05%), Query Frame = 0


Query: 25  LTLTEAAAARIRQLLQQRQRPF-LRLGVKARGCNGLSYTLNYADEKG--KFDELVEDQNV 84
           ++L+E A   + ++  +R     LR+GVK  GC+G+SYT+++ +       D  +E Q  
Sbjct: 72  ISLSENALKHLSKMRSERGEDLCLRIGVKQGGCSGMSYTMDFENRANARPDDSTIEYQGF 131

Query: 85  KILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESF 127
            I+ DPK+++ + G ++D+ D  +   F F NPN+   CGCG+SF
Sbjct: 132 TIVCDPKSMLFLFGMQLDYSDALIGGGFSFSNPNATQTCGCGKSF 176

[Close]

BLAST of MELO3C012350.2 vs. Swiss-Prot
Match: sp|Q8LBM4|ISAM1_ARATH (Iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g16710 PE=2 SV=2)

HSP 1 Score: 208.8 bits (530), Expect = 3.9e-53
Identity = 97/120 (80.83%), Postives = 114/120 (95.00%), Query Frame = 0


Query: 17  GPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELV 76
           GPA+R+Q+LTLT+ AA+R+  LLQQRQ+PFLRLGVKARGCNGLSYTLNYADEKGKFDELV
Sbjct: 15  GPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLNYADEKGKFDELV 74

Query: 77  EDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTSSAEANK 136
           E++ V+IL++PKALMHVIGTKMDFVDDKLRSEF+FINPNS+GQCGCGESFMTTS++ A +
Sbjct: 75  EEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVFINPNSQGQCGCGESFMTTSTSSAKQ 134

[Close]

BLAST of MELO3C012350.2 vs. Swiss-Prot
Match: sp|Q8L8C0|ISAM3_ARATH (Iron-sulfur assembly protein IscA-like 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g36260 PE=3 SV=2)

HSP 1 Score: 174.5 bits (441), Expect = 8.2e-43
Identity = 81/110 (73.64%), Postives = 100/110 (90.91%), Query Frame = 0


Query: 20  VRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQ 79
           +R+Q+L L++ AAARIRQLLQ +Q+PFLRL V+A+GCNGLSY LNYA EKGKFDE+VE++
Sbjct: 1   MRKQVLALSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYAQEKGKFDEVVEEK 60

Query: 80  NVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTT 130
            VKIL+DPKA+MHVIGT+MDFVDDKLRSEF+F+NPN+  +CGCGESF TT
Sbjct: 61  GVKILVDPKAVMHVIGTEMDFVDDKLRSEFVFVNPNAT-KCGCGESFTTT 109

[Close]

BLAST of MELO3C012350.2 vs. Swiss-Prot
Match: sp|Q07821|ISA1_YEAST (Iron-sulfur assembly protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 2.6e-28
Identity = 58/107 (54.21%), Postives = 78/107 (72.90%), Query Frame = 0


Query: 21  RRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQN 80
           R+ ++TL+  A   +R LL Q +   +R+  + RGC+GL+Y L Y  E GKFDE+VE   
Sbjct: 143 RKALITLSPKAIKHLRALLAQPEPKLIRVSARNRGCSGLTYDLQYITEPGKFDEVVEQDG 202

Query: 81  VKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFM 128
           VKI+ID KAL  +IG++MD++DDKL S+F+F NPNSKG CGCGESFM
Sbjct: 203 VKIVIDSKALFSIIGSEMDWIDDKLASKFVFKNPNSKGTCGCGESFM 249

[Close]

BLAST of MELO3C012350.2 vs. Swiss-Prot
Match: sp|Q8T3X9|ISCA1_DROME (Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Drosophila melanogaster OX=7227 GN=MagR PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 2.2e-27
Identity = 62/106 (58.49%), Postives = 76/106 (71.70%), Query Frame = 0


Query: 22  RQILTLTEAAAARIRQLLQQR-QRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQN 81
           R  LTLT AA  RI+ LLQ +     L++GV+ RGCNGLSYTL+YA +K K DE V    
Sbjct: 23  RAALTLTPAAVLRIKTLLQDKPDMVGLKVGVRQRGCNGLSYTLDYASQKDKLDEEVVQDG 82

Query: 82  VKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESF 127
           VK+ ID KA + ++GT+MDFV+ KL SEF+F NPN KG CGCGESF
Sbjct: 83  VKVFIDKKAQLSLLGTEMDFVESKLSSEFVFNNPNIKGTCGCGESF 128

[Close]

BLAST of MELO3C012350.2 vs. Swiss-Prot
Match: sp|Q9ZD62|Y484_RICPR (Uncharacterized protein RP484 OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=RP484 PE=3 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 1.8e-26
Identity = 50/107 (46.73%), Postives = 83/107 (77.57%), Query Frame = 0


Query: 22  RQILTLTEAAAARIRQLLQQRQRPF--LRLGVKARGCNGLSYTLNYADEKGKFDELVEDQ 81
           + +++LT+AAA +++ L+++R +P   +R+G+K+ GC G +Y + YAD K +FDE+VE++
Sbjct: 2   KNVISLTDAAAKQVKLLIEKRAKPTFGIRVGIKSGGCAGQTYYVEYADNKNQFDEVVEEK 61

Query: 82  NVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESF 127
            V+ILIDPK LM+++G++MD+V+   +S+F F NPN K  CGCG+SF
Sbjct: 62  GVRILIDPKTLMYILGSEMDYVETNFKSQFTFTNPNEKANCGCGKSF 108

[Close]

BLAST of MELO3C012350.2 vs. TrEMBL
Match: tr|A0A1S3BFV2|A0A1S3BFV2_CUCME (iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103489389 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.1e-60
Identity = 121/121 (100.00%), Postives = 121/121 (100.00%), Query Frame = 0


Query: 17  GPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELV 76
           GPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELV
Sbjct: 17  GPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELV 76

Query: 77  EDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTSSAEANK 136
           EDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTSSAEANK
Sbjct: 77  EDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTSSAEANK 136

Query: 137 K 138
           K
Sbjct: 137 K 137

[Close]

BLAST of MELO3C012350.2 vs. TrEMBL
Match: tr|A0A2P5EF11|A0A2P5EF11_9ROSA (FeS cluster insertion protein OS=Trema orientalis OX=63057 GN=TorRG33x02_200390 PE=4 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 1.8e-55
Identity = 109/121 (90.08%), Postives = 118/121 (97.52%), Query Frame = 0


Query: 17  GPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELV 76
           GPA+RRQ+L+LT+AAA RIRQLLQQRQR FLRLGVKARGCNGLSYTLNYADEKGKFDELV
Sbjct: 14  GPALRRQVLSLTDAAADRIRQLLQQRQRSFLRLGVKARGCNGLSYTLNYADEKGKFDELV 73

Query: 77  EDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTSSAEANK 136
           ED+ VKILIDPKALMHV+GTKMDFVDDKLRSEFIF+NPNSKGQCGCGESFMTTSSAEA+K
Sbjct: 74  EDKGVKILIDPKALMHVVGTKMDFVDDKLRSEFIFVNPNSKGQCGCGESFMTTSSAEASK 133

Query: 137 K 138
           +
Sbjct: 134 R 134

[Close]

BLAST of MELO3C012350.2 vs. TrEMBL
Match: tr|A0A2P5A958|A0A2P5A958_PARAD (FeS cluster insertion protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_355780 PE=4 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 5.3e-55
Identity = 108/121 (89.26%), Postives = 117/121 (96.69%), Query Frame = 0


Query: 17  GPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELV 76
           GPA+RRQ+L+LT+AAA RIRQLLQQRQR FLRLGVKARGCNGLSYTLNYADEKGKFDELV
Sbjct: 14  GPALRRQVLSLTDAAADRIRQLLQQRQRSFLRLGVKARGCNGLSYTLNYADEKGKFDELV 73

Query: 77  EDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTSSAEANK 136
           ED+ VKILIDPKALMHV+GTKMDFVDDKLRSEFIF+NPNSKGQCGCGESFMTTSSAE +K
Sbjct: 74  EDKGVKILIDPKALMHVVGTKMDFVDDKLRSEFIFVNPNSKGQCGCGESFMTTSSAETSK 133

Query: 137 K 138
           +
Sbjct: 134 R 134

[Close]

BLAST of MELO3C012350.2 vs. TrEMBL
Match: tr|A0A067DZH9|A0A067DZH9_CITSI (Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g032819mg PE=4 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 6.9e-55
Identity = 108/121 (89.26%), Postives = 116/121 (95.87%), Query Frame = 0


Query: 17  GPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELV 76
           GPA+RRQ LTLTE+AA R+RQLL+QRQRPFLRLGVKARGCNGLSYTLNYADEK KFDE+V
Sbjct: 10  GPAIRRQALTLTESAAERLRQLLEQRQRPFLRLGVKARGCNGLSYTLNYADEKSKFDEVV 69

Query: 77  EDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTSSAEANK 136
           ED+ VKILIDPKALMHVIGTKMDFVDDKLRSEF+FINPNSKGQCGCGESFMTTSSAEA K
Sbjct: 70  EDKGVKILIDPKALMHVIGTKMDFVDDKLRSEFVFINPNSKGQCGCGESFMTTSSAEAAK 129

Query: 137 K 138
           +
Sbjct: 130 R 130

[Close]

BLAST of MELO3C012350.2 vs. TrEMBL
Match: tr|V4U4F8|V4U4F8_9ROSI (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10017146mg PE=4 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.0e-54
Identity = 107/121 (88.43%), Postives = 116/121 (95.87%), Query Frame = 0


Query: 17  GPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELV 76
           GPA+RRQ LTLTE+AA R+RQLL++RQRPFLRLGVKARGCNGLSYTLNYADEK KFDE+V
Sbjct: 10  GPAIRRQALTLTESAAERLRQLLERRQRPFLRLGVKARGCNGLSYTLNYADEKSKFDEVV 69

Query: 77  EDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTSSAEANK 136
           ED+ VKILIDPKALMHVIGTKMDFVDDKLRSEF+FINPNSKGQCGCGESFMTTSSAEA K
Sbjct: 70  EDKGVKILIDPKALMHVIGTKMDFVDDKLRSEFVFINPNSKGQCGCGESFMTTSSAEAAK 129

Query: 137 K 138
           +
Sbjct: 130 R 130

The following BLAST results are available for this feature:

BLAST of MELO3C012350.2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008446757.1	1.7e-60	100.00	PREDICTED: iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucumis melo...	[more]
XP_022956980.1	1.2e-58	96.69	iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita moschata]	[more]
XP_023539778.1	2.0e-58	96.69	iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita pepo subsp. p...	[more]
PON84141.1	2.8e-55	90.08	FeS cluster insertion protein [Trema orientalis]	[more]
XP_022968348.1	6.2e-55	93.22	iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita maxima] >XP_0...	[more]

BLAST of MELO3C012350.2 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G16710.3	2.0e-52	76.98	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G36260.1	4.6e-44	73.64	Iron-sulphur cluster biosynthesis family protein	[more]
AT1G10500.1	1.4e-13	34.29	chloroplast-localized ISCA-like protein	[more]

BLAST of MELO3C012350.2 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q8LBM4\|ISAM1_ARATH	3.9e-53	80.83	Iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Arabidopsis thaliana ...	[more]
sp\|Q8L8C0\|ISAM3_ARATH	8.2e-43	73.64	Iron-sulfur assembly protein IscA-like 3, mitochondrial OS=Arabidopsis thaliana ...	[more]
sp\|Q07821\|ISA1_YEAST	2.6e-28	54.21	Iron-sulfur assembly protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 /...	[more]
sp\|Q8T3X9\|ISCA1_DROME	2.2e-27	58.49	Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Drosophila melanogaster...	[more]
sp\|Q9ZD62\|Y484_RICPR	1.8e-26	46.73	Uncharacterized protein RP484 OS=Rickettsia prowazekii (strain Madrid E) OX=2729...	[more]

BLAST of MELO3C012350.2 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3BFV2\|A0A1S3BFV2_CUCME	1.1e-60	100.00	iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucumis melo OX=3656 ...	[more]
tr\|A0A2P5EF11\|A0A2P5EF11_9ROSA	1.8e-55	90.08	FeS cluster insertion protein OS=Trema orientalis OX=63057 GN=TorRG33x02_200390 ...	[more]
tr\|A0A2P5A958\|A0A2P5A958_PARAD	5.3e-55	89.26	FeS cluster insertion protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_355...	[more]
tr\|A0A067DZH9\|A0A067DZH9_CITSI	6.9e-55	89.26	Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g032819mg PE=4 SV=1	[more]
tr\|V4U4F8\|V4U4F8_9ROSI	2.0e-54	88.43	Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10017146mg PE=4 ...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0051536	iron-sulfur cluster binding
GO:0005198	structural molecule activity

Vocabulary: **Biological Process**
Term	Definition
GO:0097428	protein maturation by iron-sulfur cluster transfer

Vocabulary: **INTERPRO**
Term	Definition
IPR017870	FeS_cluster_insertion_CS
IPR035903	HesB-like_dom_sf
IPR016092	FeS_cluster_insertion
IPR000361	FeS_biogenesis

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0097428	protein maturation by iron-sulfur cluster transfer
biological_process	GO:0016226	iron-sulfur cluster assembly
biological_process	GO:0006310	DNA recombination
biological_process	GO:0006281	DNA repair
biological_process	GO:0006260	DNA replication
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005634	nucleus
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0005198	structural molecule activity
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C012350.2.1	MELO3C012350.2.1	mRNA

InterPro

Analysis Name: InterPro Annotations of melon v3.6.1
Date Performed: 2018-09-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000361	FeS cluster biogenesis	PFAM	PF01521	Fe-S_biosyn	coord: 25..124 e-value: 1.5E-21 score: 76.6
IPR016092	FeS cluster insertion protein	TIGRFAM	TIGR00049	TIGR00049	coord: 25..127 e-value: 3.6E-35 score: 118.4
IPR035903	HesB-like domain superfamily	GENE3D	G3DSA:2.60.300.12		coord: 24..128 e-value: 4.6E-34 score: 118.6
IPR035903	HesB-like domain superfamily	SUPERFAMILY	SSF89360	HesB-like domain	coord: 24..117
None	No IPR available	PANTHER	PTHR10072	IRON-SULFUR CLUSTER ASSEMBLY PROTEIN	coord: 5..132
None	No IPR available	PANTHER	PTHR10072:SF51	SUBFAMILY NOT NAMED	coord: 5..132
IPR017870	FeS cluster insertion, C-terminal, conserved site	PROSITE	PS01152	HESB	coord: 109..126

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
MELO3C012350.2	Csa5G614650	Cucumber (Chinese Long) v2	cumedB359
MELO3C012350.2	CSPI05G26340	Wild cucumber (PI 183967)	cpimedB363
MELO3C012350.2	Cucsa.282590	Cucumber (Gy14) v1	cgymedB471
MELO3C012350.2	CmaCh18G009490	Cucurbita maxima (Rimu)	cmamedB408
MELO3C012350.2	CmoCh04G015070	Cucurbita moschata (Rifu)	cmomedB720
MELO3C012350.2	Lsi04G001520	Bottle gourd (USVL1VR-Ls)	lsimedB297
MELO3C012350.2	Cp4.1LG01g14330	Cucurbita pepo (Zucchini)	cpemedB423
MELO3C012350.2	Cp4.1LG09g03640	Cucurbita pepo (Zucchini)	cpemedB028
MELO3C012350.2	CsGy5G025800	Cucumber (Gy14) v2	cgybmedB318
MELO3C012350.2	Cla020489	Watermelon (97103) v1	medwmB061
MELO3C012350.2	ClCG05G021780	Watermelon (Charleston Gray)	medwcgB061
MELO3C012350.2	Cla97C05G103500	Watermelon (97103) v2	medwmbB056
MELO3C012350.2	Bhi07G000635	Wax gourd	medwgoB081
MELO3C012350.2	Carg18993	Silver-seed gourd	carmedB1031
MELO3C012350.2	Carg01902	Silver-seed gourd	carmedB0846
MELO3C012350.2	CsaV3_5G035380	Cucumber (Chinese Long) v3	cucmedB375

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
MELO3C012350.2	Cucurbita maxima (Rimu)	cmamedB726
MELO3C012350.2	Cucurbita moschata (Rifu)	cmomedB395
MELO3C012350.2	Cucurbita pepo (Zucchini)	cpemedB512