Carg01902 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg01902
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Iron-sulfur cluster assembly protein, putative
Location	Cucurbita_argyrosperma_scaffold_003 : 1040442 .. 1043728 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAATAAAAATTCAAAATTAACATTAACAAAATGGTGACAATTGTAATTCAACCAAAATGCCATGTAACGGAAATTCCACGAAAAAAAAAAACAAGAAAAGGTGAAAGCTGTCGAGTAAATGAACCTTTGCTTCTATTTGGAGAGGAAAAAATTGATAGGATCGTGGAGGGAAATGGCAGCTTCTTCGTCTTCCTTTTTCGCCGCTGCATCTCAGAAGGTCGGACCAGCCGCTCGCCGCCAAATTCTTACTCTGACGGAGGCCGCCGCCGCTAGAATTCGACAACTTCTTCAACAACGCCAACGTCCATTCCTCCGCCTTGGCGTCAAGGCGCGCGGTTGTAATGGTTTATCCTATACTCTCAACTACGCAGGTTCGTAATACCTAATGAATTTTAACTTTTTCTTGATTGTTTGCGATTTCACTTTACTCATTTTATGTCTGAATTTTAGGATGTGGTGAAAATTTTCGATTATGTATCCTCTTTTCTATGAATCAGATGTTTCTGTTTGAATTTGATTCGAGATAATTAGGTATTTCGTTCATTTGAGCGACATAACCTAACAGTTAAAGATGAGATGTTTCGGAACTGGAGTCCTTTAGAAGAAAAATTCTCTATCCTTTAACTGTTTCCAGCTCCAGTCACCAAGAATTATGATTTTAAGACCGAGGATTTGTTATGATTATTAGATGCAAAAAACTAGAAGGTTAAATGATTCTGTAATTGTTAAACCGGAATTGGGCCTGGGTATGGTTGGCGAGTTGTTCGAAGATGAGCGTTGTGTTCTTGATATCCTTAACAAACAGCAAATCTGAAAGATTAAACTGACATGCCTGAATGCGTCAGTTGATAGACTAAAAATGATTTTTCATGCAGGGACCTATGTATGAGAGGAGATTAACTTAACTACTTCAGTCTTAAATCATTCTGGATCTTATAGAATAGTTACGAATTTAATGTTGTTACGAGACAGGAAGTTTGGATTCGTCGATTTGCTTCACGAGCTTTTGACATAGGGATTAGTGGATTATTGATATACTACTATCCCAGACTAAATGTATGTTTCTCCCAAATTTATGGTGAGCATTTGTTTCCATCAAGTACCATTGACTTTGTGGTGTAGGTTGGCTATTGTGAGAACATAGCAGGAATGGACATCGATTAAGTTGTACTTGAGAACCTTTCTCTCTCTTTCCTGCTATGGTTAATGTGCCGGTCCAAGCCCACGGCTAGCAGATATTGTCGGCTTTTCCTTTCGGGATTCCTCTCAAGGTTTTTAAAATGCGTTTGCTAGGGAGAGGTTTCCACACCCTTATAAAGGATGCTTCGTTCTCCTCCCCAACCGACGGTGGGATCTCACAATCCCCTCCTTTGGGGTCTAGCGTCCTCACTGGCACTGACACTTGTTCCCTTCTCCAATCGATGTGGGATCTTACAATCCCCTCCTTTGGGGTCTAGCGTCCTCGCTGGCATTGACACTCGTTCCCTTCTCCAATCGATGTAGGATCTCTCAGTCCACCCCCCTTCGGAGCCCAGCGTCCTTGCGGGCACACCGCCTTGTGTCCACCCCCCTTCGGAGCCCAGCGTCCTTGCGGGCACACCGCCTTGTGTCCACCCCCTTCGGAGCTTAGCCTCCTTGCTGGTACCTTGCTTGGTGTCTAGCTCTGATACCATTTGTAATGACCCAAGTCCACTGTTAGTAGATATTGTACTATTTGGGCTTTCCCTTTTGGGTTTCCCCTCAAGGTTTTTAAAATGTGTCTCCTAGAGATAGGTTTCTACACCCTTATAAAGAATGTTTCGTTCTCCTCTCCAACCGATGTGGGATCTCACAGTTAATTAGTATGTTATTGGACCAAAGTGCTGTTTCTATTCAATTAGAAGATAAATGGGTTAACAAGCCTCTAAGGGTCAACTTGGAGAGAATAGTTATTTAACATGTTAGAAGGTCAGAAGTTTGAGATTTTATACACTGTGGGTTGGGACTTACTATTGTTTGTGTTTATTTAATATGGCTATCTTCTCAGTTAACAGTATTCACAATTCAGCTTTTTATGGCTGATTGGATAATTTTCTATCTCAGCATTACCTCAGTTTTATAATCACATTTTCCTTGTGCAAGAAATAGGATTCAACCATTATCTTTCCGATGGCATAAATGGACTCTAGTTATGATCTCCATCTTTTAAAATACTTCAATATTGCATTATTTCGTATCATCATTTTCATTTGTCAAATATTTCAGATGAGAAAGGGAAGTTTGATGAACTGGTTGAGGATCAAAACGTGAAGATACTGATTGAACCGAAGGCGCTGATGCATGTAATAGGAACAAAGATGGACTTCATAGATGACAAATTGAGGTATTTGAGAAATCTCTTACCTACAATTTTATGTTTTCAGCTCAATTATCATCCATATGTAGAGGAAATATCCTGCATGCTAGTTATTACATCAAACTGACATATAATGGCTCCTAGAAACGCTACATTGCTATTCCAAAATTCAAACATATTCTAAAAGGTCTTAATCAGCTGTGAGCTAATTCCATTCTGAATGCTCCTCAATCAATTTTCTATAGTGCTTGGAGCTATTTACAACATGCAAGGATCCCATTAATTTTTTTTTTTGCACAGAACAGTAAAAAATTCATTAGATTCCGTCTATTCGAAACTCATTAGATAAGACACAAATTAACACAAATTATCAACTCGTAGTAGGCCAATGAAACTGAGTTTAGATTCCGTCCATTCGAAACTCATTAGATAAGACACAAATTATCAACTCGTAGTAGGCCAATGAAACTGAGTTTCTCTCTTTCTTCTTGGGAGATCGGAGTTTATCTTTATCAATCCAAATTCGAAAGGTCAATGTGGCTGTGGGGAGTCCTTTATGACAACAGGCAGTGCAGAAGCCAACAAAAAATGAGGCAGTTCGAGCAATGAGCACGGTTAGAAAAACTCAAAACTCACAGTTGCATATTTGTTGTGTATTTTGCTGGAATTACAAACCCTAAACCTTAGAGAGTTCTATGACAAGAACTTAAAGCAGCTGTAAGAACACTTATCTATGGCTAATTAAGTTCTTAGAAAGCCATTTAGCCATTGTACTCCGCTAATTATTTCCTTTTCTCTTGCATGTAAATTACTGTAGATTGCACTGGCTAATGAGTTCTTCTGAGGTAATAAAATTCTCCCACGACTTTCTTTTGTTATAGGACTTGGTTACCATAACCACCCTGCTTGCCTTATAATTGGTTAAGTAATAATCAATCGGTTAAAATTTATG

mRNA sequence

CAATAAAAATTCAAAATTAACATTAACAAAATGGTGACAATTGTAATTCAACCAAAATGCCATGTAACGGAAATTCCACGAAAAAAAAAAACAAGAAAAGGTGAAAGCTGTCGAGTAAATGAACCTTTGCTTCTATTTGGAGAGGAAAAAATTGATAGGATCGTGGAGGGAAATGGCAGCTTCTTCGTCTTCCTTTTTCGCCGCTGCATCTCAGAAGGTCGGACCAGCCGCTCGCCGCCAAATTCTTACTCTGACGGAGGCCGCCGCCGCTAGAATTCGACAACTTCTTCAACAACGCCAACGTCCATTCCTCCGCCTTGGCGTCAAGGCGCGCGGTTGTAATGGTTTATCCTATACTCTCAACTACGCAGATGAGAAAGGGAAGTTTGATGAACTGGTTGAGGATCAAAACGTGAAGATACTGATTGAACCGAAGGCGCTGATGCATGTAATAGGAACAAAGATGGACTTCATAGATGACAAATTGAGATCGGAGTTTATCTTTATCAATCCAAATTCGAAAGGTCAATGTGGCTGTGGGGAGTCCTTTATGACAACAGGCAGTGCAGAAGCCAACAAAAAATGAGGCAGTTCGAGCAATGAGCACGGTTAGAAAAACTCAAAACTCACAGTTGCATATTTGTTGTGTATTTTGCTGGAATTACAAACCCTAAACCTTAGAGAGTTCTATGACAAGAACTTAAAGCAGCTGTAAGAACACTTATCTATGGCTAATTAAGTTCTTAGAAAGCCATTTAGCCATTGTACTCCGCTAATTATTTCCTTTTCTCTTGCATGTAAATTACTGTAGATTGCACTGGCTAATGAGTTCTTCTGAGGTAATAAAATTCTCCCACGACTTTCTTTTGTTATAGGACTTGGTTACCATAACCACCCTGCTTGCCTTATAATTGGTTAAGTAATAATCAATCGGTTAAAATTTATG

Coding sequence (CDS)

ATGAACCTTTGCTTCTATTTGGAGAGGAAAAAATTGATAGGATCGTGGAGGGAAATGGCAGCTTCTTCGTCTTCCTTTTTCGCCGCTGCATCTCAGAAGGTCGGACCAGCCGCTCGCCGCCAAATTCTTACTCTGACGGAGGCCGCCGCCGCTAGAATTCGACAACTTCTTCAACAACGCCAACGTCCATTCCTCCGCCTTGGCGTCAAGGCGCGCGGTTGTAATGGTTTATCCTATACTCTCAACTACGCAGATGAGAAAGGGAAGTTTGATGAACTGGTTGAGGATCAAAACGTGAAGATACTGATTGAACCGAAGGCGCTGATGCATGTAATAGGAACAAAGATGGACTTCATAGATGACAAATTGAGATCGGAGTTTATCTTTATCAATCCAAATTCGAAAGGTCAATGTGGCTGTGGGGAGTCCTTTATGACAACAGGCAGTGCAGAAGCCAACAAAAAATGA

Protein sequence

MNLCFYLERKKLIGSWREMAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFIDDKLRSEFIFINPNSKGQCGCGESFMTTGSAEANKK

Homology

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BLAST of Carg01902 vs. NCBI nr
Match: XP_022987453.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita maxima])

HSP 1 Score: 240.0 bits (611), Expect = 5.5e-60
Identity = 153/155 (98.71%), Postives = 154/155 (99.35%), Query Frame = 0


Query: 1   MNLCFYLERKKLIGSWREMAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRIRQLLQQR 60
           MNLC YLERKKLIGSWREMAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRIRQLLQQR
Sbjct: 1   MNLCVYLERKKLIGSWREMAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRIRQLLQQR 60

Query: 61  QRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFID 120
           QRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDF+D
Sbjct: 61  QRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVD 120

Query: 121 DKLRSEFIFINPNSKGQCGCGESFMTTGSAEANKK 156
           DKLRSEFIFINPNSKGQCGCGESFMTTGSAEANKK
Sbjct: 121 DKLRSEFIFINPNSKGQCGCGESFMTTGSAEANKK 155

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BLAST of Carg01902 vs. NCBI nr
Match: XP_022956980.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita moschata])

HSP 1 Score: 212.6 bits (540), Expect = 9.4e-52
Identity = 103/104 (99.04%), Postives = 104/104 (100.00%), Query Frame = 0


Query: 52  RIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHV 111
           RIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHV
Sbjct: 34  RIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHV 93

Query: 112 IGTKMDFIDDKLRSEFIFINPNSKGQCGCGESFMTTGSAEANKK 156
           IGTKMDF+DDKLRSEFIFINPNSKGQCGCGESFMTTGSAEANKK
Sbjct: 94  IGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTGSAEANKK 137

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BLAST of Carg01902 vs. NCBI nr
Match: XP_022150583.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial [Momordica charantia])

HSP 1 Score: 210.7 bits (535), Expect = 3.6e-51
Identity = 102/104 (98.08%), Postives = 104/104 (100.00%), Query Frame = 0


Query: 52  RIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHV 111
           RIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQ+VKILIEPKALMHV
Sbjct: 34  RIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQDVKILIEPKALMHV 93

Query: 112 IGTKMDFIDDKLRSEFIFINPNSKGQCGCGESFMTTGSAEANKK 156
           IGTKMDF+DDKLRSEFIFINPNSKGQCGCGESFMTTGSAEANKK
Sbjct: 94  IGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTGSAEANKK 137

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BLAST of Carg01902 vs. NCBI nr
Match: XP_023539778.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 210.3 bits (534), Expect = 4.7e-51
Identity = 102/104 (98.08%), Postives = 103/104 (99.04%), Query Frame = 0


Query: 52  RIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHV 111
           RIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHV
Sbjct: 34  RIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHV 93

Query: 112 IGTKMDFIDDKLRSEFIFINPNSKGQCGCGESFMTTGSAEANKK 156
           IGTKMDF+DDKLRSEFIFINPNSKGQCGCGESFMTTGSAEA KK
Sbjct: 94  IGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTGSAEAKKK 137

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BLAST of Carg01902 vs. NCBI nr
Match: XP_008446757.1 (PREDICTED: iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucumis melo])

HSP 1 Score: 209.1 bits (531), Expect = 1.0e-50
Identity = 101/104 (97.12%), Postives = 103/104 (99.04%), Query Frame = 0


Query: 52  RIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHV 111
           RIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILI+PKALMHV
Sbjct: 34  RIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIDPKALMHV 93

Query: 112 IGTKMDFIDDKLRSEFIFINPNSKGQCGCGESFMTTGSAEANKK 156
           IGTKMDF+DDKLRSEFIFINPNSKGQCGCGESFMTT SAEANKK
Sbjct: 94  IGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTSSAEANKK 137

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BLAST of Carg01902 vs. TAIR10
Match: AT2G16710.3 (Iron-sulphur cluster biosynthesis family protein)

HSP 1 Score: 179.5 bits (454), Expect = 1.6e-45
Identity = 85/109 (77.98%), Postives = 97/109 (88.99%), Query Frame = 0


Query: 52  RIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYA------DEKGKFDELVEDQNVKILIEP 111
           R+  LLQQRQ+PFLRLGVKARGCNGLSYTLNYA      DEKGKFDELVE++ V+IL+EP
Sbjct: 32  RVHHLLQQRQKPFLRLGVKARGCNGLSYTLNYAGKTSGNDEKGKFDELVEEKGVRILVEP 91

Query: 112 KALMHVIGTKMDFIDDKLRSEFIFINPNSKGQCGCGESFMTTGSAEANK 155
           KALMHVIGTKMDF+DDKLRSEF+FINPNS+GQCGCGESFMTT ++ A +
Sbjct: 92  KALMHVIGTKMDFVDDKLRSEFVFINPNSQGQCGCGESFMTTSTSSAKQ 140

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BLAST of Carg01902 vs. TAIR10
Match: AT2G36260.1 (Iron-sulphur cluster biosynthesis family protein)

HSP 1 Score: 158.7 bits (400), Expect = 2.9e-39
Identity = 72/96 (75.00%), Postives = 88/96 (91.67%), Query Frame = 0


Query: 52  RIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHV 111
           RIRQLLQ +Q+PFLRL V+A+GCNGLSY LNYA EKGKFDE+VE++ VKIL++PKA+MHV
Sbjct: 15  RIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYAQEKGKFDEVVEEKGVKILVDPKAVMHV 74

Query: 112 IGTKMDFIDDKLRSEFIFINPNSKGQCGCGESFMTT 148
           IGT+MDF+DDKLRSEF+F+NPN+  +CGCGESF TT
Sbjct: 75  IGTEMDFVDDKLRSEFVFVNPNAT-KCGCGESFTTT 109

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BLAST of Carg01902 vs. TAIR10
Match: AT1G10500.1 (chloroplast-localized ISCA-like protein)

HSP 1 Score: 70.1 bits (170), Expect = 1.4e-12
Identity = 31/82 (37.80%), Postives = 51/82 (62.20%), Query Frame = 0


Query: 65  LRLGVKARGCNGLSYTLNYADEKG--KFDELVEDQNVKILIEPKALMHVIGTKMDFIDDK 124
           LR+GVK  GC+G+SYT+++ +       D  +E Q   I+ +PK+++ + G ++D+ D  
Sbjct: 95  LRIGVKQGGCSGMSYTMDFENRANARPDDSTIEYQGFTIVCDPKSMLFLFGMQLDYSDAL 154

Query: 125 LRSEFIFINPNSKGQCGCGESF 145
           +   F F NPN+   CGCG+SF
Sbjct: 155 IGGGFSFSNPNATQTCGCGKSF 176

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BLAST of Carg01902 vs. Swiss-Prot
Match: sp|Q8LBM4|ISAM1_ARATH (Iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g16710 PE=2 SV=2)

HSP 1 Score: 186.0 bits (471), Expect = 3.1e-46
Identity = 85/103 (82.52%), Postives = 97/103 (94.17%), Query Frame = 0


Query: 52  RIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHV 111
           R+  LLQQRQ+PFLRLGVKARGCNGLSYTLNYADEKGKFDELVE++ V+IL+EPKALMHV
Sbjct: 32  RVHHLLQQRQKPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEEKGVRILVEPKALMHV 91

Query: 112 IGTKMDFIDDKLRSEFIFINPNSKGQCGCGESFMTTGSAEANK 155
           IGTKMDF+DDKLRSEF+FINPNS+GQCGCGESFMTT ++ A +
Sbjct: 92  IGTKMDFVDDKLRSEFVFINPNSQGQCGCGESFMTTSTSSAKQ 134

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BLAST of Carg01902 vs. Swiss-Prot
Match: sp|Q8L8C0|ISAM3_ARATH (Iron-sulfur assembly protein IscA-like 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g36260 PE=3 SV=2)

HSP 1 Score: 158.7 bits (400), Expect = 5.3e-38
Identity = 72/96 (75.00%), Postives = 88/96 (91.67%), Query Frame = 0


Query: 52  RIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHV 111
           RIRQLLQ +Q+PFLRL V+A+GCNGLSY LNYA EKGKFDE+VE++ VKIL++PKA+MHV
Sbjct: 15  RIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYAQEKGKFDEVVEEKGVKILVDPKAVMHV 74

Query: 112 IGTKMDFIDDKLRSEFIFINPNSKGQCGCGESFMTT 148
           IGT+MDF+DDKLRSEF+F+NPN+  +CGCGESF TT
Sbjct: 75  IGTEMDFVDDKLRSEFVFVNPNAT-KCGCGESFTTT 109

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BLAST of Carg01902 vs. Swiss-Prot
Match: sp|Q07821|ISA1_YEAST (Iron-sulfur assembly protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 6.0e-26
Identity = 54/93 (58.06%), Postives = 70/93 (75.27%), Query Frame = 0


Query: 53  IRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHVI 112
           +R LL Q +   +R+  + RGC+GL+Y L Y  E GKFDE+VE   VKI+I+ KAL  +I
Sbjct: 157 LRALLAQPEPKLIRVSARNRGCSGLTYDLQYITEPGKFDEVVEQDGVKIVIDSKALFSII 216

Query: 113 GTKMDFIDDKLRSEFIFINPNSKGQCGCGESFM 146
           G++MD+IDDKL S+F+F NPNSKG CGCGESFM
Sbjct: 217 GSEMDWIDDKLASKFVFKNPNSKGTCGCGESFM 249

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BLAST of Carg01902 vs. Swiss-Prot
Match: sp|Q54VS1|ISCA1_DICDI (Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=isca1 PE=3 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 7.4e-24
Identity = 48/83 (57.83%), Postives = 69/83 (83.13%), Query Frame = 0


Query: 65  LRLGVKARGCNGLSYTLNYADEKGKFDE-LVEDQNVKILIEPKALMHVIGTKMDFIDDKL 124
           LRLG+K RGC+G+SYTL++A +K KFDE +V D+++ I+++ KAL+ VIGT+MD+I++ +
Sbjct: 43  LRLGIKERGCSGMSYTLDFATQKNKFDETVVADKDINIIVDSKALLSVIGTEMDYIEEPI 102

Query: 125 RSEFIFINPNSKGQCGCGESFMT 147
           + EFIFINPN+   CGCGESF T
Sbjct: 103 KKEFIFINPNATNTCGCGESFTT 125

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BLAST of Carg01902 vs. Swiss-Prot
Match: sp|Q8T3X9|ISCA1_DROME (Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Drosophila melanogaster OX=7227 GN=MagR PE=1 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 7.4e-24
Identity = 53/94 (56.38%), Postives = 69/94 (73.40%), Query Frame = 0


Query: 52  RIRQLLQQR-QRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMH 111
           RI+ LLQ +     L++GV+ RGCNGLSYTL+YA +K K DE V    VK+ I+ KA + 
Sbjct: 35  RIKTLLQDKPDMVGLKVGVRQRGCNGLSYTLDYASQKDKLDEEVVQDGVKVFIDKKAQLS 94

Query: 112 VIGTKMDFIDDKLRSEFIFINPNSKGQCGCGESF 145
           ++GT+MDF++ KL SEF+F NPN KG CGCGESF
Sbjct: 95  LLGTEMDFVESKLSSEFVFNNPNIKGTCGCGESF 128

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BLAST of Carg01902 vs. TrEMBL
Match: tr|A0A1S3BFV2|A0A1S3BFV2_CUCME (iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103489389 PE=4 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 6.9e-51
Identity = 101/104 (97.12%), Postives = 103/104 (99.04%), Query Frame = 0


Query: 52  RIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHV 111
           RIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILI+PKALMHV
Sbjct: 34  RIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIDPKALMHV 93

Query: 112 IGTKMDFIDDKLRSEFIFINPNSKGQCGCGESFMTTGSAEANKK 156
           IGTKMDF+DDKLRSEFIFINPNSKGQCGCGESFMTT SAEANKK
Sbjct: 94  IGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTSSAEANKK 137

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BLAST of Carg01902 vs. TrEMBL
Match: tr|A0A0A0KTF0|A0A0A0KTF0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G614650 PE=4 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.2e-50
Identity = 101/103 (98.06%), Postives = 102/103 (99.03%), Query Frame = 0


Query: 53  IRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHVI 112
           IRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHVI
Sbjct: 35  IRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHVI 94

Query: 113 GTKMDFIDDKLRSEFIFINPNSKGQCGCGESFMTTGSAEANKK 156
           GTKMDF+DDKLRSEFIFINPNSKGQCGCGESFMTT SAEANKK
Sbjct: 95  GTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTSSAEANKK 137

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BLAST of Carg01902 vs. TrEMBL
Match: tr|A0A2P5EF11|A0A2P5EF11_9ROSA (FeS cluster insertion protein OS=Trema orientalis OX=63057 GN=TorRG33x02_200390 PE=4 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.2e-47
Identity = 94/104 (90.38%), Postives = 101/104 (97.12%), Query Frame = 0


Query: 52  RIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHV 111
           RIRQLLQQRQR FLRLGVKARGCNGLSYTLNYADEKGKFDELVED+ VKILI+PKALMHV
Sbjct: 31  RIRQLLQQRQRSFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDKGVKILIDPKALMHV 90

Query: 112 IGTKMDFIDDKLRSEFIFINPNSKGQCGCGESFMTTGSAEANKK 156
           +GTKMDF+DDKLRSEFIF+NPNSKGQCGCGESFMTT SAEA+K+
Sbjct: 91  VGTKMDFVDDKLRSEFIFVNPNSKGQCGCGESFMTTSSAEASKR 134

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BLAST of Carg01902 vs. TrEMBL
Match: tr|I1JQ14|I1JQ14_SOYBN (Uncharacterized protein OS=Glycine max OX=3847 GN=GLYMA_03G195000 PE=4 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 2.7e-47
Identity = 93/103 (90.29%), Postives = 101/103 (98.06%), Query Frame = 0


Query: 52  RIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHV 111
           RIRQLLQQRQRPFL+LGVKARGCNGLSYTLNYADEKGKFDELVED+ VKILI+PKALMHV
Sbjct: 31  RIRQLLQQRQRPFLKLGVKARGCNGLSYTLNYADEKGKFDELVEDKGVKILIDPKALMHV 90

Query: 112 IGTKMDFIDDKLRSEFIFINPNSKGQCGCGESFMTTGSAEANK 155
           IGTKMDF+DDKLRSEFIF+NPNSKGQCGCGESFMTTGS+ ++K
Sbjct: 91  IGTKMDFVDDKLRSEFIFVNPNSKGQCGCGESFMTTGSSGSSK 133

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BLAST of Carg01902 vs. TrEMBL
Match: tr|A0A0K9QLU5|A0A0K9QLU5_SPIOL (Uncharacterized protein OS=Spinacia oleracea OX=3562 GN=SOVF_164410 PE=4 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 3.5e-47
Identity = 94/104 (90.38%), Postives = 101/104 (97.12%), Query Frame = 0


Query: 52  RIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHV 111
           RI+QLLQQRQR FLRLGVKARGCNGLSYTLNYADEKGKFDEL+ED+ VKILIEPKALMHV
Sbjct: 30  RIQQLLQQRQRSFLRLGVKARGCNGLSYTLNYADEKGKFDELIEDKGVKILIEPKALMHV 89

Query: 112 IGTKMDFIDDKLRSEFIFINPNSKGQCGCGESFMTTGSAEANKK 156
           IGTKMDF+DDKLRSEFIFINPNSKGQCGCGESFMTT S+EA+K+
Sbjct: 90  IGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTSSSEASKQ 133

The following BLAST results are available for this feature:

BLAST of Carg01902 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022987453.1	5.5e-60	98.71	iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita maxima]	[more]
XP_022956980.1	9.4e-52	99.04	iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita moschata]	[more]
XP_022150583.1	3.6e-51	98.08	iron-sulfur assembly protein IscA-like 1, mitochondrial [Momordica charantia]	[more]
XP_023539778.1	4.7e-51	98.08	iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita pepo subsp. p...	[more]
XP_008446757.1	1.0e-50	97.12	PREDICTED: iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucumis melo...	[more]

BLAST of Carg01902 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G16710.3	1.6e-45	77.98	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G36260.1	2.9e-39	75.00	Iron-sulphur cluster biosynthesis family protein	[more]
AT1G10500.1	1.4e-12	37.80	chloroplast-localized ISCA-like protein	[more]

BLAST of Carg01902 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q8LBM4\|ISAM1_ARATH	3.1e-46	82.52	Iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Arabidopsis thaliana ...	[more]
sp\|Q8L8C0\|ISAM3_ARATH	5.3e-38	75.00	Iron-sulfur assembly protein IscA-like 3, mitochondrial OS=Arabidopsis thaliana ...	[more]
sp\|Q07821\|ISA1_YEAST	6.0e-26	58.06	Iron-sulfur assembly protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 /...	[more]
sp\|Q54VS1\|ISCA1_DICDI	7.4e-24	57.83	Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Dictyostelium discoideu...	[more]
sp\|Q8T3X9\|ISCA1_DROME	7.4e-24	56.38	Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Drosophila melanogaster...	[more]

BLAST of Carg01902 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3BFV2\|A0A1S3BFV2_CUCME	6.9e-51	97.12	iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucumis melo OX=3656 ...	[more]
tr\|A0A0A0KTF0\|A0A0A0KTF0_CUCSA	1.2e-50	98.06	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G614650 PE=4 SV=1	[more]
tr\|A0A2P5EF11\|A0A2P5EF11_9ROSA	1.2e-47	90.38	FeS cluster insertion protein OS=Trema orientalis OX=63057 GN=TorRG33x02_200390 ...	[more]
tr\|I1JQ14\|I1JQ14_SOYBN	2.7e-47	90.29	Uncharacterized protein OS=Glycine max OX=3847 GN=GLYMA_03G195000 PE=4 SV=1	[more]
tr\|A0A0K9QLU5\|A0A0K9QLU5_SPIOL	3.5e-47	90.38	Uncharacterized protein OS=Spinacia oleracea OX=3562 GN=SOVF_164410 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005198	structural molecule activity
GO:0051536	iron-sulfur cluster binding

Vocabulary: **Biological Process**
Term	Definition
GO:0097428	protein maturation by iron-sulfur cluster transfer

Vocabulary: **INTERPRO**
Term	Definition
IPR017870	FeS_cluster_insertion_CS
IPR035903	HesB-like_dom_sf
IPR016092	FeS_cluster_insertion
IPR000361	FeS_biogenesis

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0097428	protein maturation by iron-sulfur cluster transfer
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0005198	structural molecule activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg01902-RA	Carg01902-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000361	FeS cluster biogenesis	PFAM	PF01521	Fe-S_biosyn	coord: 43..142 e-value: 3.5E-20 score: 72.2
IPR016092	FeS cluster insertion protein	TIGRFAM	TIGR00049	TIGR00049	coord: 43..145 e-value: 4.1E-34 score: 114.9
IPR035903	HesB-like domain superfamily	GENE3D	G3DSA:2.60.300.12		coord: 42..146 e-value: 6.0E-33 score: 115.0
IPR035903	HesB-like domain superfamily	SUPERFAMILY	SSF89360	HesB-like domain	coord: 42..135
None	No IPR available	PANTHER	PTHR10072	IRON-SULFUR CLUSTER ASSEMBLY PROTEIN	coord: 23..149
None	No IPR available	PANTHER	PTHR10072:SF51	SUBFAMILY NOT NAMED	coord: 23..149
IPR017870	FeS cluster insertion, C-terminal, conserved site	PROSITE	PS01152	HESB	coord: 127..144

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg01902	Cla020489	Watermelon (97103) v1	carwmB0842
Carg01902	Csa5G614650	Cucumber (Chinese Long) v2	carcuB0857
Carg01902	MELO3C012350	Melon (DHL92) v3.5.1	carmeB0834
Carg01902	ClCG05G021780	Watermelon (Charleston Gray)	carwcgB0816
Carg01902	CSPI05G26340	Wild cucumber (PI 183967)	carcpiB0877
Carg01902	Cucsa.282590	Cucumber (Gy14) v1	carcgyB0968
Carg01902	CmaCh18G009490	Cucurbita maxima (Rimu)	carcmaB1167
Carg01902	CmoCh18G009510	Cucurbita moschata (Rifu)	carcmoB1135
Carg01902	Lsi04G001520	Bottle gourd (USVL1VR-Ls)	carlsiB767
Carg01902	Cp4.1LG09g03640	Cucurbita pepo (Zucchini)	carcpeB1169
Carg01902	Cp4.1LG01g14330	Cucurbita pepo (Zucchini)	carcpeB1177
Carg01902	CsGy5G025800	Cucumber (Gy14) v2	carcgybB0831
Carg01902	MELO3C012350.2	Melon (DHL92) v3.6.1	carmedB0846
Carg01902	CsaV3_5G035380	Cucumber (Chinese Long) v3	carcucB0881
Carg01902	Cla97C05G103500	Watermelon (97103) v2	carwmbB0878
Carg01902	Bhi07G000635	Wax gourd	carwgoB0979

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg01902	Carg18993	Silver-seed gourd	carcarB629

The following block(s) are covering this gene:


Gene	Organism	Block
Carg01902	Cucurbita maxima (Rimu)	carcmaB1168
Carg01902	Cucurbita maxima (Rimu)	carcmaB1170
Carg01902	Cucurbita moschata (Rifu)	carcmoB1136
Carg01902	Cucurbita moschata (Rifu)	carcmoB1138
Carg01902	Cucurbita pepo (Zucchini)	carcpeB1176
Carg01902	Bottle gourd (USVL1VR-Ls)	carlsiB769
Carg01902	Watermelon (Charleston Gray)	carwcgB0819
Carg01902	Watermelon (97103) v1	carwmB0838