Cucsa.259540 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.259540
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionCytochrome b5
Locationscaffold02229 : 5979346 .. 5981229 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGCCAAATAAGAGTCGCAAGAAATGGCTTGGCCGGTGGTGCTTCTAGTCAAATATAGAAGACAACATACCTTGCTCATTTGATTTTCTCCTTTCTTCATTTTCTTTCAAACTTTAATCTGTAAGAAACCCATCTTCTGTATTTTCAATCAGTTTTGAATTGGAGCTTCATTTTTGTTTGCACTTGATtGTTGCAGTAATGGCTTCGGATCCTAAGCTTTTCGTCTTCGATGAAGTGGCTAAACATAATCACCAGGCGGATTGTTGGCTTATAATCTCTGGAAAGGTCTGATATATTTTGTTTTTCTGGTATTTTGTGAAATTATATGTTTACAACTCAATCGTACTTCCCCATTGGAACAGTAGACTCATATGAGACCAGATTGAGGTATGGCAGATTAAGTTTCTTTGTTTCTTCTACCCATCTTCACAAACTGATAGACTGGGTATTCGGTTTAAATACTACTTTGACCGAATTTTTAACTTTGATTAAAGTGTCAGTTTCTCGAAAGTACAGGAACTAAGTAAAATACATCTTTACTGCATGGCATTTGATGCTCAAGAAACTTGTAGAGGTTGAGATACAGGACACTGATCTTTGTATAATGGAAACTAAATGCACAGAAAAGCTGTTGTTTAGCACTAGAGGAAAAATTTGGGCTTCCAAATTATTGAGTTTAGCTACTTAGTATGTGTAGGATTCGAAACTCTAACTTCTATTACCCACAGAGTCTATCTCTGAAGGTCTAGGTGAGCAGAGACTAAGAGTAATACTTGAAGAGTACATCTCAGAACTTCGACTAACATATTCTATGTAGAATATTGAAATGCTTTTATGGATTGTCGGGATGCCTGTTTAATGCAACAAGTCTCTCGGTCTCCCTTTTATACGTCTAAGTTTCAAGCATGGAACTTGGGAATGGAAGATACATTGTAAGCCATGACAACATATCACTAGAGAGTGGAATGAAATTACAGCTTCTGAAATTGTTTCTGCTGTTGTTATATCAGTACTCGTCTACCAGATATGCATATAATCAGACCTTTCTAACTTGACTGTCCAAATTTATATAGATATTTTCCTTATTTATTCGTATTGTCATTCTGTTTCACTGCTTTGTCCCTGTGCATTTCCCATGATAGGTTTATGATGTCACTCCTTTTCTGGAAGATCATCCAGGAGGTGATGAAGTCTTATTATTAGCTACTGGTAAGTTTTTGGGTCAACATGTTTTCTCCAAATTGACTACTTGTCACTGTATTTCAAGACATCCAACAAAGATGATTGCATCAAGTTATGAATGATTGGAAAAACAATTTTAAATCTGTGCAGAGAAAGATGCAACTGAAGACTTTGAGACTGTGAGTCACAGTTTAGATGCAACAGAGGAAATGGAAAAATATTATATTGGCAATATTGACATGTCTACGATTCCAAAGCCGGCTGATCATCGACCACCTGCATCCAAATCAGAATCGGCAGCAACCGAAGCAGCAACAGAAAAAACATCGTCGGCTCAATCTTCAGGATCTTTGATAAAAGTGTTGCAAGTTTTGATTCCTTTGTTGATTATAGGTGTTGCATTTTATCTGCAATACTATGGCAAAAAGCAGTAATCGACATTCTCAAATCATGGATGCCTTGAGTTTTGCAATAAGCCAGACATCTCCTCATCACATTCTGTTGAACACACTCTTATCTAGGTTTCTCTGCCCTTTTGACTCTTGCTAATTTGTGAGTAGAGAATTTTCATTTGTTTCTTGTTTCATAATCCTGCTTTACTCTGTCTTTTGTTTTACAATATTCAAGTTTCTGTGCTGATTTAACACACCAGATATATCAGGTTGAAGGTAGATGAAATAATGGATTGTCTAAATTTGGTCC

mRNA sequence

CTGCCAAATAAGAGTCGCAAGAAATGGCTTGGCCGGTGGTGCTTCTAGTCAAATATAGAAGACAACATACCTTGCTCATTTGATTTTCTCCTTTCTTCATTTTCTTTCAAACTTTAATCTTAATGGCTTCGGATCCTAAGCTTTTCGTCTTCGATGAAGTGGCTAAACATAATCACCAGGCGGATTGTTGGCTTATAATCTCTGGAAAGGTTTATGATGTCACTCCTTTTCTGGAAGATCATCCAGGAGGTGATGAAGTCTTATTATTAGCTACTGAGAAAGATGCAACTGAAGACTTTGAGACTGTGAGTCACAGTTTAGATGCAACAGAGGAAATGGAAAAATATTATATTGGCAATATTGACATGTCTACGATTCCAAAGCCGGCTGATCATCGACCACCTGCATCCAAATCAGAATCGGCAGCAACCGAAGCAGCAACAGAAAAAACATCGTCGGCTCAATCTTCAGGATCTTTGATAAAAGTGTTGCAAGTTTTGATTCCTTTGTTGATTATAGGTGTTGCATTTTATCTGCAATACTATGGCAAAAAGCAGTAATCGACATTCTCAAATCATGGATGCCTTGAGTTTTGCAATAAGCCAGACATCTCCTCATCACATTCTGTTGAACACACTCTTATCTAGGTTTCTCTGCCCTTTTGACTCTTGCTAATTTGTGAGTAGAGAATTTTCATTTGTTTCTTGTTTCATAATCCTGCTTTACTCTGTCTTTTGTTTTACAATATTCAAGTTTCTGTGCTGATTTAACACACCAGATATATCAGGTTGAAGGTAGATGAAATAATGGATTGTCTAAATTTGGTCC

Coding sequence (CDS)

ATGGCTTCGGATCCTAAGCTTTTCGTCTTCGATGAAGTGGCTAAACATAATCACCAGGCGGATTGTTGGCTTATAATCTCTGGAAAGGTTTATGATGTCACTCCTTTTCTGGAAGATCATCCAGGAGGTGATGAAGTCTTATTATTAGCTACTGAGAAAGATGCAACTGAAGACTTTGAGACTGTGAGTCACAGTTTAGATGCAACAGAGGAAATGGAAAAATATTATATTGGCAATATTGACATGTCTACGATTCCAAAGCCGGCTGATCATCGACCACCTGCATCCAAATCAGAATCGGCAGCAACCGAAGCAGCAACAGAAAAAACATCGTCGGCTCAATCTTCAGGATCTTTGATAAAAGTGTTGCAAGTTTTGATTCCTTTGTTGATTATAGGTGTTGCATTTTATCTGCAATACTATGGCAAAAAGCAGTAA

Protein sequence

MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFETVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLIKVLQVLIPLLIIGVAFYLQYYGKKQ*
Homology
[Close]
BLAST of Cucsa.259540 vs. Swiss-Prot
Match: CYB5_BRAOB (Cytochrome b5 OS=Brassica oleracea var. botrytis GN=CYB5 PE=1 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 1.1e-34
Identity = 75/145 (51.72%), Postives = 101/145 (69.66%), Query Frame = 1 

Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           MAS+ K+  F+EV++HN   DCWLIISGKVYDVTPF++DHPGGDEVLL +T KDAT DFE
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            V HS  A + MEKYYIG ID ST+        PA+++  A  + A  +  + +    +I
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTV--------PATRTYVAPVQPAYNQDKTPE---FMI 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 146
           K+LQ L+P+LI+G+A  ++ Y KK+
Sbjct: 121 KILQFLVPILILGLALVVRQYTKKE 134

[Close]
BLAST of Cucsa.259540 vs. Swiss-Prot
Match: CYB5E_ARATH (Cytochrome b5 isoform E OS=Arabidopsis thaliana GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 145.2 bits (365), Expect = 5.6e-34
Identity = 73/144 (50.69%), Postives = 101/144 (70.14%), Query Frame = 1 

Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           M+SD K+  F+EV+KHN   DCWLIISGKVYDVTPF++DHPGGDEVLL +T KDAT DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            V HS  A + M+KY+IG ID S++        PA+++  A  + A  +  + +    +I
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSV--------PATRTYVAPQQPAYNQDKTPE---FII 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKK 145
           K+LQ L+P+LI+G+A  +++Y KK
Sbjct: 121 KILQFLVPILILGLALVVRHYTKK 133

[Close]
BLAST of Cucsa.259540 vs. Swiss-Prot
Match: CYB5D_ARATH (Cytochrome B5 isoform D OS=Arabidopsis thaliana GN=CYTB5-D PE=1 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 5.6e-34
Identity = 71/143 (49.65%), Postives = 100/143 (69.93%), Query Frame = 1 

Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           M  D K+F   EV++H+   DCW++I GKVYDVT FL+DHPGGDEV+L +T KDAT+DFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            V HS  A   +++YY+G+ID +T+P  A   PP S +++ AT+         +SS  +I
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTS-TKAVATQ--------DKSSDFVI 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGK 144
           K+LQ L+PLLI+G+AF ++YY K
Sbjct: 121 KLLQFLVPLLILGLAFGIRYYTK 134

[Close]
BLAST of Cucsa.259540 vs. Swiss-Prot
Match: CYB5_TOBAC (Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 1.2e-33
Identity = 71/144 (49.31%), Postives = 98/144 (68.06%), Query Frame = 1 

Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           M  + K+F   EV++HN+  DCWL+ISGKVYDVT FL+DHPGGDEVLL AT KDAT+DFE
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            V HS  A   +++YY+G+ID +TIP    + PP     +       +KTS       ++
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYN------QDKTSE-----FVV 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKK 145
           K+LQ L+PL+I+GVAF +++Y K+
Sbjct: 121 KLLQFLVPLIILGVAFGIRFYTKQ 133

[Close]
BLAST of Cucsa.259540 vs. Swiss-Prot
Match: CYB5S_TOBAC (Cytochrome b5, seed isoform OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 2.4e-32
Identity = 71/144 (49.31%), Postives = 93/144 (64.58%), Query Frame = 1 

Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           M    K+F   EV+ HN+  DCWLIISGKVY+VT FLEDHPGG EVLL AT KDAT+DFE
Sbjct: 1   MGGQSKVFTLAEVSNHNNAKDCWLIISGKVYNVTKFLEDHPGGGEVLLSATGKDATDDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            + HS  A   +++YY+G+ID STIP    + PP  K      +  TE          ++
Sbjct: 61  DIGHSSSARAMLDEYYVGDIDSSTIPTKVKYTPP--KQPHYNQDKTTE---------FIV 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKK 145
           K+LQ L+PL+I+GVAF + +Y K+
Sbjct: 121 KLLQFLVPLIILGVAFGVHFYTKQ 133

[Close]
BLAST of Cucsa.259540 vs. TrEMBL
Match: A0A0A0L720_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G119260 PE=3 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 5.4e-76
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 1 

Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE
Sbjct: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
           TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI
Sbjct: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 146
           KVLQVLIPLLIIGVAFYLQYYGKKQ
Sbjct: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 145

[Close]
BLAST of Cucsa.259540 vs. TrEMBL
Match: M5X125_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013234mg PE=3 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 2.1e-43
Identity = 89/145 (61.38%), Postives = 109/145 (75.17%), Query Frame = 1 

Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           MASD +++ FDEVAKHNH+ DCW+IISGKVYDVTPFLEDHPGGDEVLLLA EKDAT+DF 
Sbjct: 1   MASDSQVYHFDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKDATDDFN 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            V HS  A E+MEK+Y+G +D STIP+   ++ PA  +             S QSSG ++
Sbjct: 61  DVGHSDSAKEQMEKFYVGKVDTSTIPEQPSYKLPAQTT-----------PPSGQSSGFVV 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 146
           K+LQ L+PLLI+G AF LQYYGKK+
Sbjct: 121 KLLQFLLPLLILGAAFALQYYGKKK 134

[Close]
BLAST of Cucsa.259540 vs. TrEMBL
Match: W9RDL9_9ROSA (Cytochrome b5, seed isoform OS=Morus notabilis GN=L484_023457 PE=3 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 4.3e-41
Identity = 84/145 (57.93%), Postives = 105/145 (72.41%), Query Frame = 1 

Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           MA D K+F FDEVAKHNH+ DCW+IISGKVY+VT FLE+HPGGDEVLLLA EKDAT+DFE
Sbjct: 1   MARDSKVFAFDEVAKHNHKKDCWIIISGKVYNVTEFLEEHPGGDEVLLLAAEKDATDDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            V HS  A E MEKYY+G +D+ST+P+   ++PP               + S QSSG  +
Sbjct: 61  DVGHSDTAREMMEKYYVGKVDVSTLPEKPSYKPPPQ-----------PPSQSNQSSGGSV 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 146
           K+LQ L+PLLI+ +AF L +YGK +
Sbjct: 121 KILQFLLPLLILAIAFALHFYGKSK 134

[Close]
BLAST of Cucsa.259540 vs. TrEMBL
Match: I1NCP5_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_19G258500 PE=3 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 5.3e-39
Identity = 82/145 (56.55%), Postives = 103/145 (71.03%), Query Frame = 1 

Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           MAS+PK   F+EVAKHN++ DCW+II+GKVYD+TPFL++HPGGDEVLL +T KDAT DFE
Sbjct: 1   MASNPKTLTFEEVAKHNNKKDCWIIINGKVYDITPFLDEHPGGDEVLLTSTGKDATIDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            V HS  A E MEKY+IG +D ST+P    H  P       A           QSSG ++
Sbjct: 61  DVGHSDSAIEMMEKYFIGKVDTSTLPPKVSHSLPQPTQTHGA---------GNQSSGFVV 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 146
           K+LQ L+PLLI+G+AF LQYYG+K+
Sbjct: 121 KILQFLLPLLILGLAFALQYYGQKK 136

[Close]
BLAST of Cucsa.259540 vs. TrEMBL
Match: A0A0B2RSP9_GLYSO (Cytochrome b5 isoform 1 OS=Glycine soja GN=glysoja_029033 PE=3 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 5.3e-39
Identity = 82/145 (56.55%), Postives = 103/145 (71.03%), Query Frame = 1 

Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           MAS+PK   F+EVAKHN++ DCW+II+GKVYD+TPFL++HPGGDEVLL +T KDAT DFE
Sbjct: 1   MASNPKTLTFEEVAKHNNKKDCWIIINGKVYDITPFLDEHPGGDEVLLTSTGKDATIDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            V HS  A E MEKY+IG +D ST+P    H  P       A           QSSG ++
Sbjct: 61  DVGHSDSAIEMMEKYFIGKVDTSTLPPKVSHSLPQPTQTHGA---------GNQSSGFVV 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 146
           K+LQ L+PLLI+G+AF LQYYG+K+
Sbjct: 121 KILQFLLPLLILGLAFALQYYGQKK 136

[Close]
BLAST of Cucsa.259540 vs. TAIR10
Match: AT5G53560.1 (AT5G53560.1 cytochrome B5 isoform E)

HSP 1 Score: 145.2 bits (365), Expect = 3.2e-35
Identity = 73/144 (50.69%), Postives = 101/144 (70.14%), Query Frame = 1 

Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           M+SD K+  F+EV+KHN   DCWLIISGKVYDVTPF++DHPGGDEVLL +T KDAT DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            V HS  A + M+KY+IG ID S++        PA+++  A  + A  +  + +    +I
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSV--------PATRTYVAPQQPAYNQDKTPE---FII 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKK 145
           K+LQ L+P+LI+G+A  +++Y KK
Sbjct: 121 KILQFLVPILILGLALVVRHYTKK 133

[Close]
BLAST of Cucsa.259540 vs. TAIR10
Match: AT5G48810.1 (AT5G48810.1 cytochrome B5 isoform D)

HSP 1 Score: 145.2 bits (365), Expect = 3.2e-35
Identity = 71/143 (49.65%), Postives = 100/143 (69.93%), Query Frame = 1 

Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           M  D K+F   EV++H+   DCW++I GKVYDVT FL+DHPGGDEV+L +T KDAT+DFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            V HS  A   +++YY+G+ID +T+P  A   PP S +++ AT+         +SS  +I
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTS-TKAVATQ--------DKSSDFVI 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGK 144
           K+LQ L+PLLI+G+AF ++YY K
Sbjct: 121 KLLQFLVPLLILGLAFGIRYYTK 134

[Close]
BLAST of Cucsa.259540 vs. TAIR10
Match: AT2G32720.1 (AT2G32720.1 cytochrome B5 isoform B)

HSP 1 Score: 139.4 bits (350), Expect = 1.7e-33
Identity = 70/143 (48.95%), Postives = 94/143 (65.73%), Query Frame = 1 

Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           M  + K+F   EV++HN   DCW++I+GKVY+VT FLEDHPGGD+VLL +T KDAT+DFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            V HS  A E ME+YY+G ID +TIPK   + PP     +       +KTS       +I
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYN------QDKTSE-----FII 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGK 144
           K+LQ L+PL I+G+A  ++ Y K
Sbjct: 121 KLLQFLVPLAILGLAVGIRIYTK 132

[Close]
BLAST of Cucsa.259540 vs. TAIR10
Match: AT2G46650.1 (AT2G46650.1 cytochrome B5 isoform C)

HSP 1 Score: 134.0 bits (336), Expect = 7.3e-32
Identity = 69/138 (50.00%), Postives = 91/138 (65.94%), Query Frame = 1 

Query: 7   LFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFETVSHSL 66
           L  F +VAKH  + DCW++I GKVYD++ F+++HPGGD VLL  T KDA+ DFE V+HS 
Sbjct: 4   LISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSK 63

Query: 67  DATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLIKVLQVL 126
           DA E M+KY IG++D ST+P    + PP  K ES A E   E++          K+L  L
Sbjct: 64  DAKELMKKYCIGDVDQSTVPVTQQYIPPWEK-ESTAAETTKEESGK--------KLLIYL 123

Query: 127 IPLLIIGVAFYLQYYGKK 145
           IPLLI+GVAF L++Y  K
Sbjct: 124 IPLLILGVAFALRFYNNK 132

[Close]
BLAST of Cucsa.259540 vs. TAIR10
Match: AT1G26340.1 (AT1G26340.1 cytochrome B5 isoform A)

HSP 1 Score: 108.6 bits (270), Expect = 3.3e-24
Identity = 46/82 (56.10%), Postives = 62/82 (75.61%), Query Frame = 1 

Query: 6  KLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFETVSHS 65
          KL+  +E A HN Q DCW++I GKVYDV+ ++++HPGGD+VLL    KDAT+DFE   HS
Sbjct: 6  KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 66 LDATEEMEKYYIGNIDMSTIPK 88
           DA E MEKY+IG +D S++P+
Sbjct: 66 KDARELMEKYFIGELDESSLPE 87

[Close]
BLAST of Cucsa.259540 vs. NCBI nr
Match: gi|449432012|ref|XP_004133794.1| (PREDICTED: cytochrome b5 [Cucumis sativus])

HSP 1 Score: 291.6 bits (745), Expect = 7.7e-76
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 1 

Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE
Sbjct: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
           TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI
Sbjct: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 146
           KVLQVLIPLLIIGVAFYLQYYGKKQ
Sbjct: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 145

[Close]
BLAST of Cucsa.259540 vs. NCBI nr
Match: gi|659074875|ref|XP_008437843.1| (PREDICTED: cytochrome b5-like [Cucumis melo])

HSP 1 Score: 256.1 bits (653), Expect = 3.6e-65
Identity = 129/145 (88.97%), Postives = 133/145 (91.72%), Query Frame = 1 

Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           MASDPKLFVFDEVAKHNHQ DCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE
Sbjct: 1   MASDPKLFVFDEVAKHNHQGDCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
           TV HSL ATEEMEKYYIGNIDMST+PK  D+RPPASKS S  T+AA E TSS QSSGSLI
Sbjct: 61  TVGHSLSATEEMEKYYIGNIDMSTVPK-VDYRPPASKSASTETKAAAETTSSNQSSGSLI 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 146
           KVLQ+LIPLLII VAFYLQYYGKKQ
Sbjct: 121 KVLQLLIPLLIIVVAFYLQYYGKKQ 144

[Close]
BLAST of Cucsa.259540 vs. NCBI nr
Match: gi|596010726|ref|XP_007218583.1| (hypothetical protein PRUPE_ppa013234mg [Prunus persica])

HSP 1 Score: 183.3 bits (464), Expect = 3.0e-43
Identity = 89/145 (61.38%), Postives = 109/145 (75.17%), Query Frame = 1 

Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           MASD +++ FDEVAKHNH+ DCW+IISGKVYDVTPFLEDHPGGDEVLLLA EKDAT+DF 
Sbjct: 1   MASDSQVYHFDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKDATDDFN 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            V HS  A E+MEK+Y+G +D STIP+   ++ PA  +             S QSSG ++
Sbjct: 61  DVGHSDSAKEQMEKFYVGKVDTSTIPEQPSYKLPAQTT-----------PPSGQSSGFVV 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 146
           K+LQ L+PLLI+G AF LQYYGKK+
Sbjct: 121 KLLQFLLPLLILGAAFALQYYGKKK 134

[Close]
BLAST of Cucsa.259540 vs. NCBI nr
Match: gi|645254539|ref|XP_008233085.1| (PREDICTED: cytochrome b5-like [Prunus mume])

HSP 1 Score: 181.8 bits (460), Expect = 8.6e-43
Identity = 88/145 (60.69%), Postives = 109/145 (75.17%), Query Frame = 1 

Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           MASD +++ FDEVAKHNH+ DCW+IISGKVYDVTPFLEDHPGGDEVLLLA EKDAT+DF 
Sbjct: 1   MASDSQVYHFDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKDATDDFN 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            V HS  A E+ME +Y+G +D STIP+  +++ PA  +             S QSSG ++
Sbjct: 61  DVGHSDSAKEQMENFYVGKVDTSTIPEQPNYKLPAQTT-----------PPSGQSSGFVV 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 146
           K+LQ L+PLLI+G AF LQYYGKK+
Sbjct: 121 KLLQFLLPLLILGAAFALQYYGKKK 134

[Close]
BLAST of Cucsa.259540 vs. NCBI nr
Match: gi|694412095|ref|XP_009334383.1| (PREDICTED: cytochrome b5-like [Pyrus x bretschneideri])

HSP 1 Score: 178.7 bits (452), Expect = 7.3e-42
Identity = 90/144 (62.50%), Postives = 108/144 (75.00%), Query Frame = 1 

Query: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60
           M S  +LF +DEVAKHNH+ DCW+IISGKVYDVTPFLEDHPGGDEVLLLA EKDAT+DF 
Sbjct: 1   MGSKSQLFHYDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKDATDDFN 60

Query: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120
            V HS  A E+MEK+Y+G +D STIP   +++ PA        +AA +   S QSSG L+
Sbjct: 61  DVGHSDSAREQMEKFYVGKVDTSTIPAQPNYKLPA--------QAAAQ---SEQSSGLLV 120

Query: 121 KVLQVLIPLLIIGVAFYLQYYGKK 145
           K+LQ L+PLLI+G AF LQYY KK
Sbjct: 121 KLLQFLLPLLIMGAAFALQYYNKK 133

The following BLAST results are available for this feature:
BLAST of Cucsa.259540 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CYB5_BRAOB1.1e-3451.72Cytochrome b5 OS=Brassica oleracea var. botrytis GN=CYB5 PE=1 SV=1[more]
CYB5E_ARATH5.6e-3450.69Cytochrome b5 isoform E OS=Arabidopsis thaliana GN=CYTB5-E PE=1 SV=2[more]
CYB5D_ARATH5.6e-3449.65Cytochrome B5 isoform D OS=Arabidopsis thaliana GN=CYTB5-D PE=1 SV=1[more]
CYB5_TOBAC1.2e-3349.31Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1[more]
CYB5S_TOBAC2.4e-3249.31Cytochrome b5, seed isoform OS=Nicotiana tabacum PE=2 SV=1[more]
BLAST of Cucsa.259540 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L720_CUCSA5.4e-76100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_3G119260 PE=3 SV=1[more]
M5X125_PRUPE2.1e-4361.38Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013234mg PE=3 SV=1[more]
W9RDL9_9ROSA4.3e-4157.93Cytochrome b5, seed isoform OS=Morus notabilis GN=L484_023457 PE=3 SV=1[more]
I1NCP5_SOYBN5.3e-3956.55Uncharacterized protein OS=Glycine max GN=GLYMA_19G258500 PE=3 SV=1[more]
A0A0B2RSP9_GLYSO5.3e-3956.55Cytochrome b5 isoform 1 OS=Glycine soja GN=glysoja_029033 PE=3 SV=1[more]
BLAST of Cucsa.259540 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G53560.13.2e-3550.69 cytochrome B5 isoform E[more]
AT5G48810.13.2e-3549.65 cytochrome B5 isoform D[more]
AT2G32720.11.7e-3348.95 cytochrome B5 isoform B[more]
AT2G46650.17.3e-3250.00 cytochrome B5 isoform C[more]
AT1G26340.13.3e-2456.10 cytochrome B5 isoform A[more]
BLAST of Cucsa.259540 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449432012|ref|XP_004133794.1|7.7e-76100.00PREDICTED: cytochrome b5 [Cucumis sativus][more]
gi|659074875|ref|XP_008437843.1|3.6e-6588.97PREDICTED: cytochrome b5-like [Cucumis melo][more]
gi|596010726|ref|XP_007218583.1|3.0e-4361.38hypothetical protein PRUPE_ppa013234mg [Prunus persica][more]
gi|645254539|ref|XP_008233085.1|8.6e-4360.69PREDICTED: cytochrome b5-like [Prunus mume][more]
gi|694412095|ref|XP_009334383.1|7.3e-4262.50PREDICTED: cytochrome b5-like [Pyrus x bretschneideri][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001199Cyt_B5-like_heme/steroid-bd
IPR018506Cyt_B5_heme-BS
Vocabulary: Molecular Function
TermDefinition
GO:0020037heme binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044710 single-organism metabolic process
biological_process GO:0006040 amino sugar metabolic process
biological_process GO:0008150 biological_process
biological_process GO:0019745 pentacyclic triterpenoid biosynthetic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0016126 sterol biosynthetic process
cellular_component GO:0016020 membrane
cellular_component GO:0044444 cytoplasmic part
cellular_component GO:0009535 chloroplast thylakoid membrane
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005774 vacuolar membrane
cellular_component GO:0043231 intracellular membrane-bounded organelle
cellular_component GO:0005575 cellular_component
molecular_function GO:0046872 metal ion binding
molecular_function GO:0020037 heme binding
molecular_function GO:0004128 cytochrome-b5 reductase activity, acting on NAD(P)H
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.259540.1Cucsa.259540.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001199Cytochrome b5-like heme/steroid binding domainPRINTSPR00363CYTOCHROMEB5coord: 55..62
score: 7.3E-13coord: 30..40
score: 7.3E-13coord: 68..80
score: 7.3E-13coord: 40..54
score: 7.3
IPR001199Cytochrome b5-like heme/steroid binding domainGENE3DG3DSA:3.10.120.10coord: 5..87
score: 2.0
IPR001199Cytochrome b5-like heme/steroid binding domainPFAMPF00173Cyt-b5coord: 11..80
score: 3.9
IPR001199Cytochrome b5-like heme/steroid binding domainSMARTSM01117Cyt_b5_2coord: 8..81
score: 1.1
IPR001199Cytochrome b5-like heme/steroid binding domainPROFILEPS50255CYTOCHROME_B5_2coord: 5..81
score: 26
IPR001199Cytochrome b5-like heme/steroid binding domainunknownSSF55856Cytochrome b5-like heme/steroid binding domaincoord: 4..91
score: 4.97
IPR018506Cytochrome b5, heme-binding sitePROSITEPS00191CYTOCHROME_B5_1coord: 36..43
scor
NoneNo IPR availablePANTHERPTHR19359CYTOCHROME B5coord: 1..145
score: 4.0
NoneNo IPR availablePANTHERPTHR19359:SF22CYTOCHROME B5 ISOFORM Acoord: 1..145
score: 4.0

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.259540Cla004873Watermelon (97103) v1cgywmB481
Cucsa.259540Cla021123Watermelon (97103) v1cgywmB479
Cucsa.259540Csa5G139160Cucumber (Chinese Long) v2cgycuB369
Cucsa.259540Csa3G119260Cucumber (Chinese Long) v2cgycuB366
Cucsa.259540MELO3C005907Melon (DHL92) v3.5.1cgymeB426
Cucsa.259540MELO3C005994Melon (DHL92) v3.5.1cgymeB439
Cucsa.259540ClCG01G000210Watermelon (Charleston Gray)cgywcgB446
Cucsa.259540ClCG05G000550Watermelon (Charleston Gray)cgywcgB451
Cucsa.259540CSPI03G07620Wild cucumber (PI 183967)cgycpiB383
Cucsa.259540CmaCh14G021300Cucurbita maxima (Rimu)cgycmaB0709
Cucsa.259540CmaCh06G009870Cucurbita maxima (Rimu)cgycmaB0725
Cucsa.259540CmoCh06G010160Cucurbita moschata (Rifu)cgycmoB0718
Cucsa.259540CmoCh02G011700Cucurbita moschata (Rifu)cgycmoB0712
Cucsa.259540CmoCh15G010730Cucurbita moschata (Rifu)cgycmoB0703
Cucsa.259540CmoCh14G021930Cucurbita moschata (Rifu)cgycmoB0700
Cucsa.259540Lsi05G021600Bottle gourd (USVL1VR-Ls)cgylsiB415
Cucsa.259540Lsi09G000030Bottle gourd (USVL1VR-Ls)cgylsiB413
Cucsa.259540Cp4.1LG08g00630Cucurbita pepo (Zucchini)cgycpeB0687
Cucsa.259540Cp4.1LG03g16000Cucurbita pepo (Zucchini)cgycpeB0682
Cucsa.259540Cp4.1LG05g05960Cucurbita pepo (Zucchini)cgycpeB0685
Cucsa.259540MELO3C005994.2Melon (DHL92) v3.6.1cgymedB432
Cucsa.259540CsaV3_5G000250Cucumber (Chinese Long) v3cgycucB393
Cucsa.259540CsaV3_3G007770Cucumber (Chinese Long) v3cgycucB387
Cucsa.259540Cla97C01G000230Watermelon (97103) v2cgywmbB445
Cucsa.259540Bhi12G000587Wax gourdcgywgoB507
Cucsa.259540Bhi01G000393Wax gourdcgywgoB514
Cucsa.259540Carg25714Silver-seed gourdcarcgyB0148
Cucsa.259540Carg20915Silver-seed gourdcarcgyB1110
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cucsa.259540Cucsa.202340Cucumber (Gy14) v1cgycgyB116
The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.259540Melon (DHL92) v3.6.1cgymedB420
Cucsa.259540Watermelon (97103) v2cgywmbB452
Cucsa.259540Cucurbita maxima (Rimu)cgycmaB0728
Cucsa.259540Wild cucumber (PI 183967)cgycpiB387