Bhi01G000393 (gene) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi01G000393
Type	gene
Organism	Benincasa hispida (Wax gourd)
Description	cytochrome b5-like
Location	chr1 : 9539014 .. 9541301 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGGTAAAAGAGTAGGAAAACAGGGACTGAAATGAAATTGTTTCAACAAATTCAATCGGGTTGTCTTTTAGCGCCGTGAAACCTGCCAAATAAAAGTCGCAAGAAATGGCTTGGATGGTAGTCAAATACAGAAGAAACCGTGCTGATTTGGGTCGTTTCTTCTCCAATTTCTTTCTACGTTTAATCTGTAAGAAACCCAGATTCTTCCTCAATTGTTCTGCATTTAAATCGTCTGATTCTCTAATTTAATCGACTTCTTCAGTGTGAATTGAGCTTGATTTTTGTTTGCAGTTGATTGTTGCAGAAATGGCTTCGGATCCTAAGGTTTTCGTCTTTGATGAAGTGGCTAAACACAATCACCAGCGGGATTGTTGGCTTATAATCTCTGGAAAGGTCTGATATATGTAACAATCGCTCTTATTTGTTGGGTATTTTGTAATCTCCATTTAGTAAAATTGTTTGGTTAGAACTTAATCGTATTTCCCATTGGAACAGTAGACTCATATGTGACCAGATTGAGGGATGGGAGATTAAGCTTGTTTGTTTCTTGATCTTCCTCCTACCCATCTTCACAAATTGATAATCAATGTATTCGATTTAAATATTATTTTGATCCGTGTACGGTTTTGACTAATTTCAGTCTACGTACTTTTAACTTTCCTTCATGTTGGTCACTGTAGATAGGAAATTGAATGCACAGAAAAGCTATTAGCTAGCACTGTAGAGACTGATTTCCTTTATTTCTGAAACAACTAATATACATTGTACTGAAAACAGACAGGAAGTTATTTCCTCATCTTTTCTTCTACAGTGAAAACAATTGTTCAGGATTTAGATATTAAACCTATGTAGATGTGAGACACATTTCATGGAACTCATTTAACAAACACTTGTTGGCAAGACTCTGGTATTGTTAACTTTGCAATGAGTAAGCTCCAACAATCAAAGAAGAATGTTAGGGCCTTGAAGTTATTTGGAGTTTAACTACATAATATGAATGCATATTCAAAACTCTGACCTTTTAAAGGTCTATCTTTAAAAGTCTATCTTTAATTTAAAACTCTGACCTTTTAATAATATTAGTGATTCTATCTTTAAAGGTCTAGGTGAACAGAAAGTAATACTTGAAGAATATATCCCAAAACTTCAATTAACGTATTCTGTATAGAACATTGAGATTTTTTTATGGATCATCGGAATACCCTGTTCAATGCTACAAGTCTTGGGGATGGAAGACACATTGCAAGCCATGGCAACATATCACTACAGAGTGTAGTGAAGTTGCAGTTTCTGAAATTGTTTGTGCTGTTGTTATATCAGTACTCATCTCCCAGATATACATATAATCAGACCTTTCCAACTTGGGTGCATATATATATATATATATCCTTACTCCTTGATATTGTCATTGTGTTTCATTGCTTTGTCCCTGTGCATTTCCCATTGTAGGTTTATGATGTCACCCCTTTTCTGGAAGAACATCCTGGAGGTGATGAAGTCTTGTTATTAGCTACTGGTAAGTTTTGGCTCAACATTTTTTTCCCAACTTGACTACTTGTTACTGTATTTCAACATATCCAGAAAAGGTTATTGCATCAAGTTATGAATGATTGGAAAAACAATCTTGAATCTTTGCAGAAAAAGATGCAACCGATGACTTTGAAACTGTGGGTCACAGTTTATCTGCAACAGAGGAAATGGAAAAATATTATGTTGGGAATGTTGACATGTCTACGGTTCCAAAGCCAGTCGATTATCGACCAGCTGCTTCCCAATCAGCAAAGGCATCAGCAACGGCAACAATAACAACGTCGTCAGCTCAATCTTCAGGATCTTTGATAAAAGTATTGCAATATCTGATTCCCCTGTTGATTATAGGTGTTGCATTTTATCTGCAATACTATGGCAAAAAACAGTAATCGAATTTCTCAAACCATAAATGTCTTGAGTTTTGCAATAAGCCAGAGCTGTGAAGACATTCTATCACTTTCTGTTGAAGACATTCTGTGTCATCTAGGTTTATAGGTTTCTTCCTAATTTTTTAGCAGAGAATTTCAATTTGTTTCTTGTTCATTCATAATCCTGCTTTTCTGTCTTTTGTTCTACAATATTCGACTTTCTGTGCATGATTTAACACAATTGACTCATCAGATATATCAGGTTGAATGAAGATGAAATAATGGGTTGTCTGAATTGAGATTCTTTCCCACTTGCCAACCCTTGTTGAGGGAATTATACTGGTGTTTTAATGAAGTGGCTTTCAAAGTACAGAGATGAGAATGACAAGC

mRNA sequence

TTGGTAAAAGAGTAGGAAAACAGGGACTGAAATGAAATTGTTTCAACAAATTCAATCGGGTTGTCTTTTAGCGCCGTGAAACCTGCCAAATAAAAGTCGCAAGAAATGGCTTGGATGGTAGTCAAATACAGAAGAAACCGTGCTGATTTGGGTCGTTTCTTCTCCAATTTCTTTCTACGTTTAATCTTTGATTGTTGCAGAAATGGCTTCGGATCCTAAGGTTTTCGTCTTTGATGAAGTGGCTAAACACAATCACCAGCGGGATTGTTGGCTTATAATCTCTGGAAAGGTTTATGATGTCACCCCTTTTCTGGAAGAACATCCTGGAGGTGATGAAGTCTTGTTATTAGCTACTGAAAAAGATGCAACCGATGACTTTGAAACTGTGGGTCACAGTTTATCTGCAACAGAGGAAATGGAAAAATATTATGTTGGGAATGTTGACATGTCTACGGTTCCAAAGCCAGTCGATTATCGACCAGCTGCTTCCCAATCAGCAAAGGCATCAGCAACGGCAACAATAACAACGTCGTCAGCTCAATCTTCAGGATCTTTGATAAAAGTATTGCAATATCTGATTCCCCTGTTGATTATAGGTGTTGCATTTTATCTGCAATACTATGGCAAAAAACAGTAATCGAATTTCTCAAACCATAAATGTCTTGAGTTTTGCAATAAGCCAGAGCTGTGAAGACATTCTATCACTTTCTGTTGAAGACATTCTGTGTCATCTAGGTTTATAGGTTTCTTCCTAATTTTTTAGCAGAGAATTTCAATTTGTTTCTTGTTCATTCATAATCCTGCTTTTCTGTCTTTTGTTCTACAATATTCGACTTTCTGTGCATGATTTAACACAATTGACTCATCAGATATATCAGGTTGAATGAAGATGAAATAATGGGTTGTCTGAATTGAGATTCTTTCCCACTTGCCAACCCTTGTTGAGGGAATTATACTGGTGTTTTAATGAAGTGGCTTTCAAAGTACAGAGATGAGAATGACAAGC

Coding sequence (CDS)

ATGGCTTCGGATCCTAAGGTTTTCGTCTTTGATGAAGTGGCTAAACACAATCACCAGCGGGATTGTTGGCTTATAATCTCTGGAAAGGTTTATGATGTCACCCCTTTTCTGGAAGAACATCCTGGAGGTGATGAAGTCTTGTTATTAGCTACTGAAAAAGATGCAACCGATGACTTTGAAACTGTGGGTCACAGTTTATCTGCAACAGAGGAAATGGAAAAATATTATGTTGGGAATGTTGACATGTCTACGGTTCCAAAGCCAGTCGATTATCGACCAGCTGCTTCCCAATCAGCAAAGGCATCAGCAACGGCAACAATAACAACGTCGTCAGCTCAATCTTCAGGATCTTTGATAAAAGTATTGCAATATCTGATTCCCCTGTTGATTATAGGTGTTGCATTTTATCTGCAATACTATGGCAAAAAACAGTAA

Protein sequence

MASDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLATEKDATDDFETVGHSLSATEEMEKYYVGNVDMSTVPKPVDYRPAASQSAKASATATITTSSAQSSGSLIKVLQYLIPLLIIGVAFYLQYYGKKQ

Homology

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BLAST of Bhi01G000393 vs. TAIR10
Match: AT5G53560.1 (cytochrome B5 isoform E)

HSP 1 Score: 149.8 bits (377), Expect = 1.3e-36
Identity = 74/147 (50.34%), Postives = 103/147 (70.07%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLATEKDATDDFE 60
           M+SD KV  F+EV+KHN  +DCWLIISGKVYDVTPF+++HPGGDEVLL +T KDAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  TVGHSLSATEEMEKYYVGNVDMSTVPKPVDY----RPAASQSXXXXXXXXXXXXXXXSSG 120
            VGHS +A + M+KY++G +D S+VP    Y    +PA +Q                +  
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQD--------------KTPE 120

Query: 121 SLIKVLQYLIPLLIIGVAFYLQYYGKK 144
            +IK+LQ+L+P+LI+G+A  +++Y KK
Sbjct: 121 FIIKILQFLVPILILGLALVVRHYTKK 133

[Close]

BLAST of Bhi01G000393 vs. TAIR10
Match: AT5G48810.1 (cytochrome B5 isoform D)

HSP 1 Score: 146.4 bits (368), Expect = 1.4e-35
Identity = 70/142 (49.30%), Postives = 96/142 (67.61%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLATEKDATDDFE 60
           M  D KVF   EV++H+  +DCW++I GKVYDVT FL++HPGGDEV+L +T KDATDDFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  TVGHSLSATEEMEKYYVGNVDMSTVPKPVDYRPAASQSXXXXXXXXXXXXXXXSSGSLIK 120
            VGHS +A   +++YYVG++D +TVP    + P  S                 SS  +IK
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTK--------AVATQDKSSDFVIK 120

Query: 121 VLQYLIPLLIIGVAFYLQYYGK 143
           +LQ+L+PLLI+G+AF ++YY K
Sbjct: 121 LLQFLVPLLILGLAFGIRYYTK 134

[Close]

BLAST of Bhi01G000393 vs. TAIR10
Match: AT2G32720.1 (cytochrome B5 isoform B)

HSP 1 Score: 144.8 bits (364), Expect = 4.1e-35
Identity = 70/142 (49.30%), Postives = 94/142 (66.20%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLATEKDATDDFE 60
           M  + K+F   EV++HN   DCW++I+GKVY+VT FLE+HPGGD+VLL +T KDATDDFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  TVGHSLSATEEMEKYYVGNVDMSTVPKPVDYRPAASQSXXXXXXXXXXXXXXXSSGSLIK 120
            VGHS SA E ME+YYVG +D +T+PK V Y P                    +S  +IK
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQ----------PHYNQDKTSEFIIK 120

Query: 121 VLQYLIPLLIIGVAFYLQYYGK 143
           +LQ+L+PL I+G+A  ++ Y K
Sbjct: 121 LLQFLVPLAILGLAVGIRIYTK 132

[Close]

BLAST of Bhi01G000393 vs. TAIR10
Match: AT2G46650.1 (cytochrome B5 isoform C)

HSP 1 Score: 124.8 bits (312), Expect = 4.3e-29
Identity = 66/134 (49.25%), Postives = 83/134 (61.94%), Query Frame = 0


Query: 10  FDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLATEKDATDDFETVGHSLSAT 69
           F +VAKH  + DCW++I GKVYD++ F++EHPGGD VLL  T KDA+ DFE V HS  A 
Sbjct: 7   FHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSKDAK 66

Query: 70  EEMEKYYVGNVDMSTVPKPVDYRPAASQSXXXXXXXXXXXXXXXSSGSLIKVLQYLIPLL 129
           E M+KY +G+VD STVP    Y P   +                 SG   K+L YLIPLL
Sbjct: 67  ELMKKYCIGDVDQSTVPVTQQYIPPWEKE------STAAETTKEESGK--KLLIYLIPLL 126

Query: 130 IIGVAFYLQYYGKK 144
           I+GVAF L++Y  K
Sbjct: 127 ILGVAFALRFYNNK 132

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BLAST of Bhi01G000393 vs. TAIR10
Match: AT1G26340.1 (cytochrome B5 isoform A)

HSP 1 Score: 111.3 bits (277), Expect = 5.0e-25
Identity = 46/82 (56.10%), Postives = 62/82 (75.61%), Query Frame = 0


Query: 6  KVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLATEKDATDDFETVGHS 65
          K++  +E A HN Q DCW++I GKVYDV+ +++EHPGGD+VLL    KDATDDFE  GHS
Sbjct: 6  KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 66 LSATEEMEKYYVGNVDMSTVPK 88
            A E MEKY++G +D S++P+
Sbjct: 66 KDARELMEKYFIGELDESSLPE 87

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BLAST of Bhi01G000393 vs. Swiss-Prot
Match: sp|P40934|CYB5_BRAOB (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 4.6e-36
Identity = 76/148 (51.35%), Postives = 103/148 (69.59%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLATEKDATDDFE 60
           MAS+ KV  F+EV++HN  +DCWLIISGKVYDVTPF+++HPGGDEVLL +T KDAT+DFE
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  TVGHSLSATEEMEKYYVGNVDMSTVPKPVDY----RPAASQSXXXXXXXXXXXXXXXSSG 120
            VGHS +A + MEKYY+G +D STVP    Y    +PA +Q                +  
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVPATRTYVAPVQPAYNQD--------------KTPE 120

Query: 121 SLIKVLQYLIPLLIIGVAFYLQYYGKKQ 145
            +IK+LQ+L+P+LI+G+A  ++ Y KK+
Sbjct: 121 FMIKILQFLVPILILGLALVVRQYTKKE 134

[Close]

BLAST of Bhi01G000393 vs. Swiss-Prot
Match: sp|Q42342|CYB5E_ARATH (Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 149.8 bits (377), Expect = 2.3e-35
Identity = 74/147 (50.34%), Postives = 103/147 (70.07%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLATEKDATDDFE 60
           M+SD KV  F+EV+KHN  +DCWLIISGKVYDVTPF+++HPGGDEVLL +T KDAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  TVGHSLSATEEMEKYYVGNVDMSTVPKPVDY----RPAASQSXXXXXXXXXXXXXXXSSG 120
            VGHS +A + M+KY++G +D S+VP    Y    +PA +Q                +  
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQD--------------KTPE 120

Query: 121 SLIKVLQYLIPLLIIGVAFYLQYYGKK 144
            +IK+LQ+L+P+LI+G+A  +++Y KK
Sbjct: 121 FIIKILQFLVPILILGLALVVRHYTKK 133

[Close]

BLAST of Bhi01G000393 vs. Swiss-Prot
Match: sp|P49098|CYB5_TOBAC (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 3.0e-35
Identity = 71/143 (49.65%), Postives = 98/143 (68.53%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLATEKDATDDFE 60
           M  + KVF   EV++HN+ +DCWL+ISGKVYDVT FL++HPGGDEVLL AT KDATDDFE
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  TVGHSLSATEEMEKYYVGNVDMSTVPKPVDYRPAASQSXXXXXXXXXXXXXXXSSGSLIK 120
            VGHS SA   +++YYVG++D +T+P    Y P                    +S  ++K
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTP----------PNQPHYNQDKTSEFVVK 120

Query: 121 VLQYLIPLLIIGVAFYLQYYGKK 144
           +LQ+L+PL+I+GVAF +++Y K+
Sbjct: 121 LLQFLVPLIILGVAFGIRFYTKQ 133

[Close]

BLAST of Bhi01G000393 vs. Swiss-Prot
Match: sp|Q9ZWT2|CYB5D_ARATH (Cytochrome B5 isoform D OS=Arabidopsis thaliana OX=3702 GN=CYTB5-D PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 2.5e-34
Identity = 70/142 (49.30%), Postives = 96/142 (67.61%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLATEKDATDDFE 60
           M  D KVF   EV++H+  +DCW++I GKVYDVT FL++HPGGDEV+L +T KDATDDFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  TVGHSLSATEEMEKYYVGNVDMSTVPKPVDYRPAASQSXXXXXXXXXXXXXXXSSGSLIK 120
            VGHS +A   +++YYVG++D +TVP    + P  S                 SS  +IK
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTK--------AVATQDKSSDFVIK 120

Query: 121 VLQYLIPLLIIGVAFYLQYYGK 143
           +LQ+L+PLLI+G+AF ++YY K
Sbjct: 121 LLQFLVPLLILGLAFGIRYYTK 134

[Close]

BLAST of Bhi01G000393 vs. Swiss-Prot
Match: sp|P49099|CYB5S_TOBAC (Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 2.5e-34
Identity = 71/143 (49.65%), Postives = 95/143 (66.43%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLATEKDATDDFE 60
           M    KVF   EV+ HN+ +DCWLIISGKVY+VT FLE+HPGG EVLL AT KDATDDFE
Sbjct: 1   MGGQSKVFTLAEVSNHNNAKDCWLIISGKVYNVTKFLEDHPGGGEVLLSATGKDATDDFE 60

Query: 61  TVGHSLSATEEMEKYYVGNVDMSTVPKPVDYRPAASQSXXXXXXXXXXXXXXXSSGSLIK 120
            +GHS SA   +++YYVG++D ST+P  V Y P                    ++  ++K
Sbjct: 61  DIGHSSSARAMLDEYYVGDIDSSTIPTKVKYTPPKQ----------PHYNQDKTTEFIVK 120

Query: 121 VLQYLIPLLIIGVAFYLQYYGKK 144
           +LQ+L+PL+I+GVAF + +Y K+
Sbjct: 121 LLQFLVPLIILGVAFGVHFYTKQ 133

[Close]

BLAST of Bhi01G000393 vs. TrEMBL
Match: tr|A0A0A0L720|A0A0A0L720_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G119260 PE=3 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.2e-58
Identity = 123/145 (84.83%), Postives = 131/145 (90.34%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLATEKDATDDFE 60
           MASDPK+FVFDEVAKHNHQ DCWLIISGKVYDVTPFLE+HPGGDEVLLLATEKDAT+DFE
Sbjct: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 61  TVGHSLSATEEMEKYYVGNVDMSTVPKPVDYRPAASQS-XXXXXXXXXXXXXXXSSGSLI 120
           TV HSL ATEEMEKYY+GN+DMST+PKP D+RP AS+S      XXXXXXXX  SSGSLI
Sbjct: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEXXXXXXXXAQSSGSLI 120

Query: 121 KVLQYLIPLLIIGVAFYLQYYGKKQ 145
           KVLQ LIPLLIIGVAFYLQYYGKKQ
Sbjct: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 145

[Close]

BLAST of Bhi01G000393 vs. TrEMBL
Match: tr|A0A1S3AV33|A0A1S3AV33_CUCME (cytochrome b5-like OS=Cucumis melo OX=3656 GN=LOC103483149 PE=3 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 4.0e-45
Identity = 89/98 (90.82%), Postives = 95/98 (96.94%), Query Frame = 0


Query: 1  MASDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLATEKDATDDFE 60
          MASDPK+FVFDEVAKHNHQ DCWLIISGKVYDVTPFLE+HPGGDEVLLLATEKDAT+DFE
Sbjct: 1  MASDPKLFVFDEVAKHNHQGDCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 61 TVGHSLSATEEMEKYYVGNVDMSTVPKPVDYRPAASQS 99
          TVGHSLSATEEMEKYY+GN+DMSTVPK VDYRP AS+S
Sbjct: 61 TVGHSLSATEEMEKYYIGNIDMSTVPK-VDYRPPASKS 97

[Close]

BLAST of Bhi01G000393 vs. TrEMBL
Match: tr|M5X125|M5X125_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_2G194100 PE=3 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 3.4e-44
Identity = 91/144 (63.19%), Postives = 110/144 (76.39%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLATEKDATDDFE 60
           MASD +V+ FDEVAKHNH++DCW+IISGKVYDVTPFLE+HPGGDEVLLLA EKDATDDF 
Sbjct: 1   MASDSQVYHFDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKDATDDFN 60

Query: 61  TVGHSLSATEEMEKYYVGNVDMSTVPKPVDYRPAASQSXXXXXXXXXXXXXXXSSGSLIK 120
            VGHS SA E+MEK+YVG VD ST+P+   Y+  A  +               SSG ++K
Sbjct: 61  DVGHSDSAKEQMEKFYVGKVDTSTIPEQPSYKLPAQTT----------PPSGQSSGFVVK 120

Query: 121 VLQYLIPLLIIGVAFYLQYYGKKQ 145
           +LQ+L+PLLI+G AF LQYYGKK+
Sbjct: 121 LLQFLLPLLILGAAFALQYYGKKK 134

[Close]

BLAST of Bhi01G000393 vs. TrEMBL
Match: tr|A0A2P5FIE4|A0A2P5FIE4_9ROSA (Nitrate reductase NADH dependent OS=Trema orientalis OX=63057 GN=TorRG33x02_067620 PE=3 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 3.8e-43
Identity = 89/144 (61.81%), Postives = 108/144 (75.00%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLATEKDATDDFE 60
           MAS  KVF FDEVA+HNH++DCW+IISGK YDVT FLEEHPGGDEVLLLA EKDATDDFE
Sbjct: 1   MASGSKVFTFDEVAQHNHKKDCWIIISGKAYDVTEFLEEHPGGDEVLLLAAEKDATDDFE 60

Query: 61  TVGHSLSATEEMEKYYVGNVDMSTVPKPVDYRPAASQSXXXXXXXXXXXXXXXSSGSLIK 120
            VGHS  A E ++KY VG VD+ST+P+  +Y+P    S               SSGS +K
Sbjct: 61  DVGHSDDAKELLKKYCVGEVDLSTLPEKPNYKPPTQSS-----------QSDQSSGSAVK 120

Query: 121 VLQYLIPLLIIGVAFYLQYYGKKQ 145
           +LQ+L+PLLI+G+AF LQ+YGKK+
Sbjct: 121 ILQFLLPLLILGIAFALQFYGKKK 133

[Close]

BLAST of Bhi01G000393 vs. TrEMBL
Match: tr|A0A2P5DQA7|A0A2P5DQA7_PARAD (Nitrate reductase NADH dependent OS=Parasponia andersonii OX=3476 GN=PanWU01x14_040920 PE=3 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 4.9e-43
Identity = 89/144 (61.81%), Postives = 108/144 (75.00%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLATEKDATDDFE 60
           MAS  KVF FDEVA+HNH++DCW+IISGK YDVT FLEEHPGGDEVLLLA EKDATDDFE
Sbjct: 1   MASGSKVFTFDEVAQHNHKKDCWIIISGKAYDVTEFLEEHPGGDEVLLLAAEKDATDDFE 60

Query: 61  TVGHSLSATEEMEKYYVGNVDMSTVPKPVDYRPAASQSXXXXXXXXXXXXXXXSSGSLIK 120
            VGHS  A E ++KY VG VD+ST+P+  +Y+P    S               SSGS +K
Sbjct: 61  DVGHSDDAKELLKKYCVGEVDLSTLPEKPNYKPPTHSS----------SQSDQSSGSAVK 120

Query: 121 VLQYLIPLLIIGVAFYLQYYGKKQ 145
           +LQ+L+PLLI+G+AF LQ+YGKK+
Sbjct: 121 ILQFLLPLLILGIAFALQFYGKKK 134

[Close]

BLAST of Bhi01G000393 vs. NCBI nr
Match: XP_004133794.1 (PREDICTED: cytochrome b5 [Cucumis sativus] >XP_011650661.1 PREDICTED: cytochrome b5 [Cucumis sativus] >KGN56392.1 hypothetical protein Csa_3G119260 [Cucumis sativus])

HSP 1 Score: 233.4 bits (594), Expect = 4.8e-58
Identity = 123/145 (84.83%), Postives = 131/145 (90.34%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLATEKDATDDFE 60
           MASDPK+FVFDEVAKHNHQ DCWLIISGKVYDVTPFLE+HPGGDEVLLLATEKDAT+DFE
Sbjct: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 61  TVGHSLSATEEMEKYYVGNVDMSTVPKPVDYRPAASQS-XXXXXXXXXXXXXXXSSGSLI 120
           TV HSL ATEEMEKYY+GN+DMST+PKP D+RP AS+S      XXXXXXXX  SSGSLI
Sbjct: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEXXXXXXXXAQSSGSLI 120

Query: 121 KVLQYLIPLLIIGVAFYLQYYGKKQ 145
           KVLQ LIPLLIIGVAFYLQYYGKKQ
Sbjct: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 145

[Close]

BLAST of Bhi01G000393 vs. NCBI nr
Match: XP_022147466.1 (cytochrome b5-like [Momordica charantia])

HSP 1 Score: 230.3 bits (586), Expect = 4.1e-57
Identity = 111/144 (77.08%), Postives = 123/144 (85.42%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLATEKDATDDFE 60
           M SDPKVF FDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVL+LA+EKDATDDFE
Sbjct: 1   MGSDPKVFAFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLILASEKDATDDFE 60

Query: 61  TVGHSLSATEEMEKYYVGNVDMSTVPKPVDYRPAASQSXXXXXXXXXXXXXXXSSGSLIK 120
            VGHSLSA ++ME+YYVGNVDMSTVPKP+DYRP ASQS               SSGS +K
Sbjct: 61  NVGHSLSAIDQMEQYYVGNVDMSTVPKPIDYRPPASQS-------ATTLASAQSSGSSLK 120

Query: 121 VLQYLIPLLIIGVAFYLQYYGKKQ 145
           +LQ+LIPLL+IGVAFY+Q+YGKKQ
Sbjct: 121 LLQFLIPLLLIGVAFYMQFYGKKQ 137

[Close]

BLAST of Bhi01G000393 vs. NCBI nr
Match: XP_023540248.1 (cytochrome b5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 230.3 bits (586), Expect = 4.1e-57
Identity = 114/144 (79.17%), Postives = 122/144 (84.72%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLATEKDATDDFE 60
           M SDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLA EKDATDDFE
Sbjct: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFE 60

Query: 61  TVGHSLSATEEMEKYYVGNVDMSTVPKPVDYRPAASQSXXXXXXXXXXXXXXXSSGSLIK 120
            VGHSLSA E+MEKYY+GNVDMSTVPKP+DYR AASQS               SSGSL+ 
Sbjct: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQS------VKATTSSAESSGSLLT 120

Query: 121 VLQYLIPLLIIGVAFYLQYYGKKQ 145
           +LQ L+PLLI+GVAFYLQ+YGKKQ
Sbjct: 121 LLQILVPLLIMGVAFYLQFYGKKQ 138

[Close]

BLAST of Bhi01G000393 vs. NCBI nr
Match: XP_022931217.1 (cytochrome B5-like [Cucurbita moschata] >XP_022974834.1 cytochrome B5-like [Cucurbita maxima] >XP_022974835.1 cytochrome B5-like [Cucurbita maxima])

HSP 1 Score: 227.3 bits (578), Expect = 3.4e-56
Identity = 113/144 (78.47%), Postives = 121/144 (84.03%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLATEKDATDDFE 60
           M SDPKVFVFDEVAKHNHQRDCWLIISGKVYDVT FLEEHPGGDEVLLLA EKDATDDFE
Sbjct: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE 60

Query: 61  TVGHSLSATEEMEKYYVGNVDMSTVPKPVDYRPAASQSXXXXXXXXXXXXXXXSSGSLIK 120
            VGHSLSA E+MEKYY+GNVDMSTVPKP+DYR AASQS               SSGSL+ 
Sbjct: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQS------VKATTSSAESSGSLLT 120

Query: 121 VLQYLIPLLIIGVAFYLQYYGKKQ 145
           +LQ L+PLLI+GVAFYLQ+YGKKQ
Sbjct: 121 LLQILVPLLIMGVAFYLQFYGKKQ 138

[Close]

BLAST of Bhi01G000393 vs. NCBI nr
Match: XP_022975276.1 (cytochrome B5-like [Cucurbita maxima])

HSP 1 Score: 226.9 bits (577), Expect = 4.5e-56
Identity = 112/144 (77.78%), Postives = 121/144 (84.03%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLATEKDATDDFE 60
           M SDPKVFVFDEVAKHNHQRDCWLIISGKVYDVT FLEEHPGGDEVLLLA EKDATDDFE
Sbjct: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE 60

Query: 61  TVGHSLSATEEMEKYYVGNVDMSTVPKPVDYRPAASQSXXXXXXXXXXXXXXXSSGSLIK 120
            VGHSLSA E+MEKYY+GNVDMST+PKP+DYR AASQS               SSGSL+ 
Sbjct: 61  NVGHSLSAIEQMEKYYIGNVDMSTIPKPIDYRLAASQS------VKATTSSAESSGSLLT 120

Query: 121 VLQYLIPLLIIGVAFYLQYYGKKQ 145
           +LQ L+PLLI+GVAFYLQ+YGKKQ
Sbjct: 121 LLQILVPLLIMGVAFYLQFYGKKQ 138

The following BLAST results are available for this feature:

BLAST of Bhi01G000393 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G53560.1	1.3e-36	50.34	cytochrome B5 isoform E	[more]
AT5G48810.1	1.4e-35	49.30	cytochrome B5 isoform D	[more]
AT2G32720.1	4.1e-35	49.30	cytochrome B5 isoform B	[more]
AT2G46650.1	4.3e-29	49.25	cytochrome B5 isoform C	[more]
AT1G26340.1	5.0e-25	56.10	cytochrome B5 isoform A	[more]

BLAST of Bhi01G000393 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|P40934\|CYB5_BRAOB	4.6e-36	51.35	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]
sp\|Q42342\|CYB5E_ARATH	2.3e-35	50.34	Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2	[more]
sp\|P49098\|CYB5_TOBAC	3.0e-35	49.65	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
sp\|Q9ZWT2\|CYB5D_ARATH	2.5e-34	49.30	Cytochrome B5 isoform D OS=Arabidopsis thaliana OX=3702 GN=CYTB5-D PE=1 SV=1	[more]
sp\|P49099\|CYB5S_TOBAC	2.5e-34	49.65	Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]

BLAST of Bhi01G000393 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0L720\|A0A0A0L720_CUCSA	3.2e-58	84.83	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G119260 PE=3 SV=1	[more]
tr\|A0A1S3AV33\|A0A1S3AV33_CUCME	4.0e-45	90.82	cytochrome b5-like OS=Cucumis melo OX=3656 GN=LOC103483149 PE=3 SV=1	[more]
tr\|M5X125\|M5X125_PRUPE	3.4e-44	63.19	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_2G194100 PE=3 SV=1	[more]
tr\|A0A2P5FIE4\|A0A2P5FIE4_9ROSA	3.8e-43	61.81	Nitrate reductase NADH dependent OS=Trema orientalis OX=63057 GN=TorRG33x02_0676...	[more]
tr\|A0A2P5DQA7\|A0A2P5DQA7_PARAD	4.9e-43	61.81	Nitrate reductase NADH dependent OS=Parasponia andersonii OX=3476 GN=PanWU01x14_...	[more]

BLAST of Bhi01G000393 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004133794.1	4.8e-58	84.83	PREDICTED: cytochrome b5 [Cucumis sativus] >XP_011650661.1 PREDICTED: cytochrome...	[more]
XP_022147466.1	4.1e-57	77.08	cytochrome b5-like [Momordica charantia]	[more]
XP_023540248.1	4.1e-57	79.17	cytochrome b5-like [Cucurbita pepo subsp. pepo]	[more]
XP_022931217.1	3.4e-56	78.47	cytochrome B5-like [Cucurbita moschata] >XP_022974834.1 cytochrome B5-like [Cucu...	[more]
XP_022975276.1	4.5e-56	77.78	cytochrome B5-like [Cucurbita maxima]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding

Vocabulary: **INTERPRO**
Term	Definition
IPR018506	Cyt_B5_heme-BS
IPR036400	Cyt_B5-like_heme/steroid_sf
IPR001199	Cyt_B5-like_heme/steroid-bd

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044710	single-organism metabolic process
biological_process	GO:0006040	amino sugar metabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0008150	biological_process
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0044444	cytoplasmic part
cellular_component	GO:0043231	intracellular membrane-bounded organelle
cellular_component	GO:0016020	membrane
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004128	cytochrome-b5 reductase activity, acting on NAD(P)H
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi01M000393	Bhi01M000393	mRNA

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 40..54 score: 47.47 coord: 55..62 score: 44.32 coord: 68..80 score: 30.07 coord: 30..40 score: 66.53
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 e-value: 8.3E-27 score: 105.1
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 11..80 e-value: 2.9E-23 score: 81.7
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 27.181
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	G3DSA:3.10.120.10		coord: 3..90 e-value: 4.9E-31 score: 108.6
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	SSF55856	Cytochrome b5-like heme/steroid binding domain	coord: 4..91
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..143
None	No IPR available	PANTHER	PTHR19359:SF43	CYTOCHROME B5 ISOFORM C	coord: 1..143
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Bhi01G000393	Csa3G119260	Cucumber (Chinese Long) v2	cuwgoB284
Bhi01G000393	MELO3C005994	Melon (DHL92) v3.5.1	mewgoB540
Bhi01G000393	CSPI03G07620	Wild cucumber (PI 183967)	cpiwgoB284
Bhi01G000393	Cucsa.259540	Cucumber (Gy14) v1	cgywgoB514
Bhi01G000393	CmaCh14G021300	Cucurbita maxima (Rimu)	cmawgoB0315
Bhi01G000393	CmoCh14G021930	Cucurbita moschata (Rifu)	cmowgoB0303
Bhi01G000393	CmoCh06G010160	Cucurbita moschata (Rifu)	cmowgoB1013
Bhi01G000393	Cp4.1LG03g16000	Cucurbita pepo (Zucchini)	cpewgoB0770
Bhi01G000393	Cp4.1LG08g00630	Cucurbita pepo (Zucchini)	cpewgoB1105
Bhi01G000393	MELO3C005994.2	Melon (DHL92) v3.6.1	medwgoB542
Bhi01G000393	Carg25714	Silver-seed gourd	carwgoB0153
Bhi01G000393	Carg20915	Silver-seed gourd	carwgoB1162
Bhi01G000393	CsaV3_3G007770	Cucumber (Chinese Long) v3	cucwgoB288

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Bhi01G000393	Bhi12G000587	Wax gourd	wgowgoB026

The following block(s) are covering this gene:


Gene	Organism	Block
Bhi01G000393	Cucumber (Gy14) v2	cgybwgoB272
Bhi01G000393	Cucurbita maxima (Rimu)	cmawgoB1011
Bhi01G000393	Bottle gourd (USVL1VR-Ls)	lsiwgoB433
Bhi01G000393	Watermelon (Charleston Gray)	wcgwgoB439
Bhi01G000393	Watermelon (97103) v2	wgowmbB191
Bhi01G000393	Watermelon (97103) v1	wgowmB212