Cp4.1LG08g00630 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG08g00630
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	Cytochrome b5
Location	Cp4.1LG08 : 4042170 .. 4044971 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGAAATTAAAGAAAATATTGGGCTTTTCTTGCAAAAAGTTGCAAAAGCAGGGACTAAAATGAAATTCTCGCAACAAATTCATTGGGCGTTTTTTAGCTCCACCAAGCCGGCCAGATAAAAGCCTTTTTAAAATGGCTGGTTGTCATTACCGGAAGAAATCGCGCTGATTTGGGTCTTTTCTCCAATTTCCCTTCAACGCTTAATCCGTAAGAAACTCATCTTCTTCCTCATTGCTTCTGTGTTTGAATCGTCTGATTCTCTAATTTAATCGATTGCTTCAGTGTGGATTGAGCTTGACTTTTCTTTGCATTTCAGTTGAAATGGATTCGGATCCTAAGGTTTTTGTCTTCGACGAAGTGGCTAAACACAATCACCAGCGGGATTGTTGGCTTATAATCTCTGGAAAGGTCTGATTATCATAATCTCTCCTCATTTTTAATCTCCATATGGAAATTTTGTTTGCTCTGAACTCAATCGTAACTGCTACTGGAACTCTAGACTTATATAACAGTAGATAGAGGGATGGATGATTAAGAAACTGATGATTGTGTATTCTATTATTGTTCTTGTTCTTTGATATCTCGGCGAGTTACCGGGCGAGCATCTGACTTACTGTTTGACCCTATCACATCTGACAATTGATTGTCAATATAGTGTTTGACCTTATCACATTTGATTCTCAAGAAAACTCAGTAGAGGTTTAACATCTTAGGGAGATGACCCATATGAGATTTGAAATTATTACTGGAGAAAACTTTAATACTCCAAGGCCACCATTAGTAGATATTGTCTATTTTAGCCCGTTATGTATTCCTGTCCGCCTCACGGTTTTAGAACGCGTCTATTAGGGAGAGGTTTCCACACCCTTATAAAAAAAATATTTCGTTCTCCTCTCCAATAGAGATGGGATCTCACAATCCACCCCCTTGGGAGCCAATGTCCTTACTGGCACACTGCCTAGTATCTGGCTCTGATACCATTTGTAATATCCAAACCCACCGCTAGCAAACATTGTCTTCATTGAGTTTTCCCATTCGAGCTTCCCCTCAAGGTTTTTATAACGTCCAAGGCCACCGTTTATAGATATTGTCCGCTTAAGCTCGTTACTTATCGTCGTCAACCTCACGTTTTTAGAACGTAGGGAGAGGTTTCTACACCCTCATAAGAAATGCTTTGTTTCCCTCTCCAATCAAGGTGGGATCTCATAATCCATCTCCCTTGGGGGCCAACGTCCTCGCTAGTACGCCGTCTGGTGTCTGGCTCTGATACCATTTGTAACACCCCAAACTCATCGCTAGCAAATATTGTCTTCTTTGGGCTTTCGCTCTCAAGTTTCCCCTCAAGATTTTTAAAACACGTATGCTAGGAGAGGTTTCCACACCCTTATTAAGAGTATTTCGTTCTCCTCCCCAACCGATGTGGGATCTCACAAGAAGGGTGAGAGAGGACGTATGACATTTGTAGAGTGCTTGAACTAGGGGTAAATTGTTTATGATAGATAAGAAACTGAGTTAGAACTATGAGACTGATACCCTTTATTTCTGGAGCAACTGATATAGATATTGTACAGAAACAGACAGGAACTTATTGCTCACTTAGTCTAAGCTCCTATTTCGTCGAAGCAGAATTTAAGGCCTAGAAAGGCTTTTAGGCTGACTAGAGAGTAATACTTTGATTAACAGGTCATTGGAATCCCTGTTTTATGTTGAAAATCTTTGGGGCTCTCATTTATGATTCTAAGTTTTAAATTATAAGCATGGAACGTGAGGATATTAATGGGATACATTGTAAGCCATGGCAGCATATCACTAAAGAGTCGAATTAAGTTGCAGGCCCAAGTTTTATCAAGGCTCTTGACATGAAATTCTTGGGATTTGATCTAACAACTTTGTTCTTTGTTAAAGAACGAGATCTCTAAATCTAAATTATCCAAAAACCCCAAAAATGGTAAAGAATCAATTCAAAGGAAAGTTTTGGAATGGATTACTTTAATAATCAAGATCAAGAGTAAAGAAAACTCGAATCACTCAAGATAACTTCCCTATTCCTTATATAGTCGTCGTGTTTCACTGCTCTGTCACGAAGTAATTATTGTGCATTCCCATTTAGGTATATGATGTGACCCCTTTTCTGGAAGAACATCCTGGAGGTGATGAAGTCTTATTATTAGCTGCGGGTATGTTTCAGCTCATATCTCGATTGGTTGTTAAAAATGATTACACCAAGTTATGAATGATCGGAAAAACAATGTTGAGTTTATGCAGAGAAAGATGCAACAGATGATTTTGAAAATGTGGGTCACAGTCTATCTGCAATAGAGCAAATGGAAAAGTATTACATTGGCAATGTTGACATGTCTACGGTCCCAAAACCGATCGATTATCGACTAGCTGCATCCCAATCGGTGAAAGCAACGACGTCGTCGGCTGAATCTTCAGGATCCTTGCTAACACTACTGCAAATTCTGGTTCCCCTGCTGATTATGGGCGTTGCATTTTATCTGCAATTCTATGGCAAAAAGCAGTAATCATTAGCTTCCTACCAAAGATGCCACGAGATTTTCAGTTACTCACTTTATGTTGAAGACATTGGTATTACGTAGGTTTCTCTGCCATTACCTCTTGCTAATTTCTGAGTAGATAATTTAAAAACAGTTCATTTGTGTCTTGTTCATTCATTATCCTGTTTTCCTTTGTCTTTTGTTCTACAATATTCAACTTTCTGTGCTTGATTTAACACCATTGACTCACCAGATATATGAAGTTGAATGAAGATCAATTAATGGGTTGTGTACAAAATGAG

mRNA sequence

CGAAATTAAAGAAAATATTGGGCTTTTCTTGCAAAAAGTTGCAAAAGCAGGGACTAAAATGAAATTCTCGCAACAAATTCATTGGGCGTTTTTTAGCTCCACCAAGCCGGCCAGATAAAAGCCTTTTTAAAATGGCTGGTTGTCATTACCGGAAGAAATCGCGCTGATTTGGGTCTTTTCTCCAATTTCCCTTCAACGCTTAATCCTTGAAATGGATTCGGATCCTAAGGTTTTTGTCTTCGACGAAGTGGCTAAACACAATCACCAGCGGGATTGTTGGCTTATAATCTCTGGAAAGGTATATGATGTGACCCCTTTTCTGGAAGAACATCCTGGAGGTGATGAAGTCTTATTATTAGCTGCGGAGAAAGATGCAACAGATGATTTTGAAAATGTGGGTCACAGTCTATCTGCAATAGAGCAAATGGAAAAGTATTACATTGGCAATGTTGACATGTCTACGGTCCCAAAACCGATCGATTATCGACTAGCTGCATCCCAATCGGTGAAAGCAACGACGTCGTCGGCTGAATCTTCAGGATCCTTGCTAACACTACTGCAAATTCTGGTTCCCCTGCTGATTATGGGCGTTGCATTTTATCTGCAATTCTATGGCAAAAAGCAGTAATCATTAGCTTCCTACCAAAGATGCCACGAGATTTTCAGTTACTCACTTTATGTTGAAGACATTGGTATTACGTAGGTTTCTCTGCCATTACCTCTTGCTAATTTCTGAGTAGATAATTTAAAAACAGTTCATTTGTGTCTTGTTCATTCATTATCCTGTTTTCCTTTGTCTTTTGTTCTACAATATTCAACTTTCTGTGCTTGATTTAACACCATTGACTCACCAGATATATGAAGTTGAATGAAGATCAATTAATGGGTTGTGTACAAAATGAG

Coding sequence (CDS)

ATGGATTCGGATCCTAAGGTTTTTGTCTTCGACGAAGTGGCTAAACACAATCACCAGCGGGATTGTTGGCTTATAATCTCTGGAAAGGTATATGATGTGACCCCTTTTCTGGAAGAACATCCTGGAGGTGATGAAGTCTTATTATTAGCTGCGGAGAAAGATGCAACAGATGATTTTGAAAATGTGGGTCACAGTCTATCTGCAATAGAGCAAATGGAAAAGTATTACATTGGCAATGTTGACATGTCTACGGTCCCAAAACCGATCGATTATCGACTAGCTGCATCCCAATCGGTGAAAGCAACGACGTCGTCGGCTGAATCTTCAGGATCCTTGCTAACACTACTGCAAATTCTGGTTCCCCTGCTGATTATGGGCGTTGCATTTTATCTGCAATTCTATGGCAAAAAGCAGTAA

Protein sequence

MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILVPLLIMGVAFYLQFYGKKQ

Homology

[Close]

BLAST of Cp4.1LG08g00630 vs. Swiss-Prot
Match: CYB5D_ARATH (Cytochrome B5 isoform D OS=Arabidopsis thaliana GN=CYTB5-D PE=1 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 1.8e-34
Identity = 68/136 (50.00%), Postives = 98/136 (72.06%), Query Frame = 1


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFE 60
           M  D KVF   EV++H+  +DCW++I GKVYDVT FL++HPGGDEV+L +  KDATDDFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS +A   +++YY+G++D +TV  P+  +     S KA  +  +SS  ++ LLQ LV
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATV--PVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLV 120

Query: 121 PLLIMGVAFYLQFYGK 137
           PLLI+G+AF +++Y K
Sbjct: 121 PLLILGLAFGIRYYTK 134

[Close]

BLAST of Cp4.1LG08g00630 vs. Swiss-Prot
Match: CYB5_BRAOB (Cytochrome b5 OS=Brassica oleracea var. botrytis GN=CYB5 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 4.0e-34
Identity = 72/138 (52.17%), Postives = 99/138 (71.74%), Query Frame = 1


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFE 60
           M S+ KV  F+EV++HN  +DCWLIISGKVYDVTPF+++HPGGDEVLL +  KDAT+DFE
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS +A + MEKYYIG +D STVP    Y       V+   +  ++   ++ +LQ LV
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVPATRTY----VAPVQPAYNQDKTPEFMIKILQFLV 120

Query: 121 PLLIMGVAFYLQFYGKKQ 139
           P+LI+G+A  ++ Y KK+
Sbjct: 121 PILILGLALVVRQYTKKE 134

[Close]

BLAST of Cp4.1LG08g00630 vs. Swiss-Prot
Match: CYB5_TOBAC (Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 5.3e-34
Identity = 70/137 (51.09%), Postives = 99/137 (72.26%), Query Frame = 1


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFE 60
           M  + KVF   EV++HN+ +DCWL+ISGKVYDVT FL++HPGGDEVLL A  KDATDDFE
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS SA   +++YY+G++D +T+P    Y    +   +   +  ++S  ++ LLQ LV
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKY----TPPNQPHYNQDKTSEFVVKLLQFLV 120

Query: 121 PLLIMGVAFYLQFYGKK 138
           PL+I+GVAF ++FY K+
Sbjct: 121 PLIILGVAFGIRFYTKQ 133

[Close]

BLAST of Cp4.1LG08g00630 vs. Swiss-Prot
Match: CYB5E_ARATH (Cytochrome b5 isoform E OS=Arabidopsis thaliana GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 144.8 bits (364), Expect = 6.9e-34
Identity = 73/137 (53.28%), Postives = 98/137 (71.53%), Query Frame = 1


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFE 60
           M SD KV  F+EV+KHN  +DCWLIISGKVYDVTPF+++HPGGDEVLL +  KDAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS +A + M+KY+IG +D S+VP    Y +A  Q       + E    ++ +LQ LV
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTY-VAPQQPAYNQDKTPE---FIIKILQFLV 120

Query: 121 PLLIMGVAFYLQFYGKK 138
           P+LI+G+A  ++ Y KK
Sbjct: 121 PILILGLALVVRHYTKK 133

[Close]

BLAST of Cp4.1LG08g00630 vs. Swiss-Prot
Match: CYB5S_TOBAC (Cytochrome b5, seed isoform OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 4.5e-33
Identity = 69/137 (50.36%), Postives = 96/137 (70.07%), Query Frame = 1


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFE 60
           M    KVF   EV+ HN+ +DCWLIISGKVY+VT FLE+HPGG EVLL A  KDATDDFE
Sbjct: 1   MGGQSKVFTLAEVSNHNNAKDCWLIISGKVYNVTKFLEDHPGGGEVLLSATGKDATDDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           ++GHS SA   +++YY+G++D ST+P  + Y    +   +   +  +++  ++ LLQ LV
Sbjct: 61  DIGHSSSARAMLDEYYVGDIDSSTIPTKVKY----TPPKQPHYNQDKTTEFIVKLLQFLV 120

Query: 121 PLLIMGVAFYLQFYGKK 138
           PL+I+GVAF + FY K+
Sbjct: 121 PLIILGVAFGVHFYTKQ 133

[Close]

BLAST of Cp4.1LG08g00630 vs. TrEMBL
Match: A0A0A0L720_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G119260 PE=3 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 1.0e-55
Identity = 109/145 (75.17%), Postives = 124/145 (85.52%), Query Frame = 1


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFE 60
           M SDPK+FVFDEVAKHNHQ DCWLIISGKVYDVTPFLE+HPGGDEVLLLA EKDAT+DFE
Sbjct: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKAT-------TSSAESSGSLL 120
            V HSL A E+MEKYYIGN+DMST+PKP D+R  AS+S  A        TSSA+SSGSL+
Sbjct: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120

Query: 121 TLLQILVPLLIMGVAFYLQFYGKKQ 139
            +LQ+L+PLLI+GVAFYLQ+YGKKQ
Sbjct: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 145

[Close]

BLAST of Cp4.1LG08g00630 vs. TrEMBL
Match: M5X125_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013234mg PE=3 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 2.7e-45
Identity = 92/138 (66.67%), Postives = 112/138 (81.16%), Query Frame = 1


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFE 60
           M SD +V+ FDEVAKHNH++DCW+IISGKVYDVTPFLE+HPGGDEVLLLA EKDATDDF 
Sbjct: 1   MASDSQVYHFDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKDATDDFN 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS SA EQMEK+Y+G VD ST+P+   Y+L A    + T  S +SSG ++ LLQ L+
Sbjct: 61  DVGHSDSAKEQMEKFYVGKVDTSTIPEQPSYKLPA----QTTPPSGQSSGFVVKLLQFLL 120

Query: 121 PLLIMGVAFYLQFYGKKQ 139
           PLLI+G AF LQ+YGKK+
Sbjct: 121 PLLILGAAFALQYYGKKK 134

[Close]

BLAST of Cp4.1LG08g00630 vs. TrEMBL
Match: W9RDL9_9ROSA (Cytochrome b5, seed isoform OS=Morus notabilis GN=L484_023457 PE=3 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 8.2e-42
Identity = 87/138 (63.04%), Postives = 107/138 (77.54%), Query Frame = 1


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFE 60
           M  D KVF FDEVAKHNH++DCW+IISGKVY+VT FLEEHPGGDEVLLLAAEKDATDDFE
Sbjct: 1   MARDSKVFAFDEVAKHNHKKDCWIIISGKVYNVTEFLEEHPGGDEVLLLAAEKDATDDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS +A E MEKYY+G VD+ST+P+   Y+       +  + S +SSG  + +LQ L+
Sbjct: 61  DVGHSDTAREMMEKYYVGKVDVSTLPEKPSYK----PPPQPPSQSNQSSGGSVKILQFLL 120

Query: 121 PLLIMGVAFYLQFYGKKQ 139
           PLLI+ +AF L FYGK +
Sbjct: 121 PLLILAIAFALHFYGKSK 134

[Close]

BLAST of Cp4.1LG08g00630 vs. TrEMBL
Match: I1NCP5_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_19G258500 PE=3 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 1.3e-39
Identity = 81/138 (58.70%), Postives = 108/138 (78.26%), Query Frame = 1


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFE 60
           M S+PK   F+EVAKHN+++DCW+II+GKVYD+TPFL+EHPGGDEVLL +  KDAT DFE
Sbjct: 1   MASNPKTLTFEEVAKHNNKKDCWIIINGKVYDITPFLDEHPGGDEVLLTSTGKDATIDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS SAIE MEKY+IG VD ST+P  + + L   Q  +   +  +SSG ++ +LQ L+
Sbjct: 61  DVGHSDSAIEMMEKYFIGKVDTSTLPPKVSHSL--PQPTQTHGAGNQSSGFVVKILQFLL 120

Query: 121 PLLIMGVAFYLQFYGKKQ 139
           PLLI+G+AF LQ+YG+K+
Sbjct: 121 PLLILGLAFALQYYGQKK 136

[Close]

BLAST of Cp4.1LG08g00630 vs. TrEMBL
Match: A0A0B2RSP9_GLYSO (Cytochrome b5 isoform 1 OS=Glycine soja GN=glysoja_029033 PE=3 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 1.3e-39
Identity = 81/138 (58.70%), Postives = 108/138 (78.26%), Query Frame = 1


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFE 60
           M S+PK   F+EVAKHN+++DCW+II+GKVYD+TPFL+EHPGGDEVLL +  KDAT DFE
Sbjct: 1   MASNPKTLTFEEVAKHNNKKDCWIIINGKVYDITPFLDEHPGGDEVLLTSTGKDATIDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS SAIE MEKY+IG VD ST+P  + + L   Q  +   +  +SSG ++ +LQ L+
Sbjct: 61  DVGHSDSAIEMMEKYFIGKVDTSTLPPKVSHSL--PQPTQTHGAGNQSSGFVVKILQFLL 120

Query: 121 PLLIMGVAFYLQFYGKKQ 139
           PLLI+G+AF LQ+YG+K+
Sbjct: 121 PLLILGLAFALQYYGQKK 136

[Close]

BLAST of Cp4.1LG08g00630 vs. TAIR10
Match: AT5G48810.1 (AT5G48810.1 cytochrome B5 isoform D)

HSP 1 Score: 146.7 bits (369), Expect = 1.0e-35
Identity = 68/136 (50.00%), Postives = 98/136 (72.06%), Query Frame = 1


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFE 60
           M  D KVF   EV++H+  +DCW++I GKVYDVT FL++HPGGDEV+L +  KDATDDFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS +A   +++YY+G++D +TV  P+  +     S KA  +  +SS  ++ LLQ LV
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATV--PVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLV 120

Query: 121 PLLIMGVAFYLQFYGK 137
           PLLI+G+AF +++Y K
Sbjct: 121 PLLILGLAFGIRYYTK 134

[Close]

BLAST of Cp4.1LG08g00630 vs. TAIR10
Match: AT5G53560.1 (AT5G53560.1 cytochrome B5 isoform E)

HSP 1 Score: 144.8 bits (364), Expect = 3.9e-35
Identity = 73/137 (53.28%), Postives = 98/137 (71.53%), Query Frame = 1


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFE 60
           M SD KV  F+EV+KHN  +DCWLIISGKVYDVTPF+++HPGGDEVLL +  KDAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS +A + M+KY+IG +D S+VP    Y +A  Q       + E    ++ +LQ LV
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTY-VAPQQPAYNQDKTPE---FIIKILQFLV 120

Query: 121 PLLIMGVAFYLQFYGKK 138
           P+LI+G+A  ++ Y KK
Sbjct: 121 PILILGLALVVRHYTKK 133

[Close]

BLAST of Cp4.1LG08g00630 vs. TAIR10
Match: AT2G32720.1 (AT2G32720.1 cytochrome B5 isoform B)

HSP 1 Score: 139.8 bits (351), Expect = 1.3e-33
Identity = 66/136 (48.53%), Postives = 95/136 (69.85%), Query Frame = 1


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFE 60
           M  + K+F   EV++HN   DCW++I+GKVY+VT FLE+HPGGD+VLL +  KDATDDFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS SA E ME+YY+G +D +T+PK + Y    +   +   +  ++S  ++ LLQ LV
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKY----TPPKQPHYNQDKTSEFIIKLLQFLV 120

Query: 121 PLLIMGVAFYLQFYGK 137
           PL I+G+A  ++ Y K
Sbjct: 121 PLAILGLAVGIRIYTK 132

[Close]

BLAST of Cp4.1LG08g00630 vs. TAIR10
Match: AT2G46650.1 (AT2G46650.1 cytochrome B5 isoform C)

HSP 1 Score: 127.5 bits (319), Expect = 6.4e-30
Identity = 66/128 (51.56%), Postives = 84/128 (65.62%), Query Frame = 1


Query: 10  FDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVGHSLSAI 69
           F +VAKH  + DCW++I GKVYD++ F++EHPGGD VLL    KDA+ DFE+V HS  A 
Sbjct: 7   FHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSKDAK 66

Query: 70  EQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILVPLLIMGVAF 129
           E M+KY IG+VD STVP    Y     +   A  ++ E SG    LL  L+PLLI+GVAF
Sbjct: 67  ELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGK--KLLIYLIPLLILGVAF 126

Query: 130 YLQFYGKK 138
            L+FY  K
Sbjct: 127 ALRFYNNK 132

[Close]

BLAST of Cp4.1LG08g00630 vs. TAIR10
Match: AT1G26340.1 (AT1G26340.1 cytochrome B5 isoform A)

HSP 1 Score: 114.4 bits (285), Expect = 5.6e-26
Identity = 48/82 (58.54%), Postives = 64/82 (78.05%), Query Frame = 1


Query: 6  KVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVGHS 65
          K++  +E A HN Q DCW++I GKVYDV+ +++EHPGGD+VLL  A KDATDDFE+ GHS
Sbjct: 6  KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 66 LSAIEQMEKYYIGNVDMSTVPK 88
            A E MEKY+IG +D S++P+
Sbjct: 66 KDARELMEKYFIGELDESSLPE 87

[Close]

BLAST of Cp4.1LG08g00630 vs. NCBI nr
Match: gi|449432012|ref|XP_004133794.1| (PREDICTED: cytochrome b5 [Cucumis sativus])

HSP 1 Score: 224.2 bits (570), Expect = 1.4e-55
Identity = 109/145 (75.17%), Postives = 124/145 (85.52%), Query Frame = 1


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFE 60
           M SDPK+FVFDEVAKHNHQ DCWLIISGKVYDVTPFLE+HPGGDEVLLLA EKDAT+DFE
Sbjct: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKAT-------TSSAESSGSLL 120
            V HSL A E+MEKYYIGN+DMST+PKP D+R  AS+S  A        TSSA+SSGSL+
Sbjct: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLI 120

Query: 121 TLLQILVPLLIMGVAFYLQFYGKKQ 139
            +LQ+L+PLLI+GVAFYLQ+YGKKQ
Sbjct: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 145

[Close]

BLAST of Cp4.1LG08g00630 vs. NCBI nr
Match: gi|659074875|ref|XP_008437843.1| (PREDICTED: cytochrome b5-like [Cucumis melo])

HSP 1 Score: 219.2 bits (557), Expect = 4.6e-54
Identity = 110/145 (75.86%), Postives = 124/145 (85.52%), Query Frame = 1


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFE 60
           M SDPK+FVFDEVAKHNHQ DCWLIISGKVYDVTPFLE+HPGGDEVLLLA EKDAT+DFE
Sbjct: 1   MASDPKLFVFDEVAKHNHQGDCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKA-------TTSSAESSGSLL 120
            VGHSLSA E+MEKYYIGN+DMSTVPK +DYR  AS+S          TTSS +SSGSL+
Sbjct: 61  TVGHSLSATEEMEKYYIGNIDMSTVPK-VDYRPPASKSASTETKAAAETTSSNQSSGSLI 120

Query: 121 TLLQILVPLLIMGVAFYLQFYGKKQ 139
            +LQ+L+PLLI+ VAFYLQ+YGKKQ
Sbjct: 121 KVLQLLIPLLIIVVAFYLQYYGKKQ 144

[Close]

BLAST of Cp4.1LG08g00630 vs. NCBI nr
Match: gi|596010726|ref|XP_007218583.1| (hypothetical protein PRUPE_ppa013234mg [Prunus persica])

HSP 1 Score: 189.5 bits (480), Expect = 3.9e-45
Identity = 92/138 (66.67%), Postives = 112/138 (81.16%), Query Frame = 1


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFE 60
           M SD +V+ FDEVAKHNH++DCW+IISGKVYDVTPFLE+HPGGDEVLLLA EKDATDDF 
Sbjct: 1   MASDSQVYHFDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKDATDDFN 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS SA EQMEK+Y+G VD ST+P+   Y+L A    + T  S +SSG ++ LLQ L+
Sbjct: 61  DVGHSDSAKEQMEKFYVGKVDTSTIPEQPSYKLPA----QTTPPSGQSSGFVVKLLQFLL 120

Query: 121 PLLIMGVAFYLQFYGKKQ 139
           PLLI+G AF LQ+YGKK+
Sbjct: 121 PLLILGAAFALQYYGKKK 134

[Close]

BLAST of Cp4.1LG08g00630 vs. NCBI nr
Match: gi|657968938|ref|XP_008376183.1| (PREDICTED: cytochrome b5-like [Malus domestica])

HSP 1 Score: 188.7 bits (478), Expect = 6.7e-45
Identity = 93/137 (67.88%), Postives = 109/137 (79.56%), Query Frame = 1


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFE 60
           M S  ++F +DEVAKHNH++DCW+IISGKVYDVTPFLEEHPGGDEVLLLA EKDATDDF 
Sbjct: 1   MGSKSQJFQYDEVAKHNHKKDCWIIISGKVYDVTPFLEEHPGGDEVLLLAVEKDATDDFN 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS SA EQMEKYY+G VD ST+P   +Y+L A    +A   S +SSG L+ LLQ L+
Sbjct: 61  DVGHSDSAREQMEKYYVGKVDTSTIPAQPNYKLPA----QAAAQSEQSSGLLVKLLQFLL 120

Query: 121 PLLIMGVAFYLQFYGKK 138
           PLLIMG AF LQ+Y KK
Sbjct: 121 PLLIMGAAFALQYYNKK 133

[Close]

BLAST of Cp4.1LG08g00630 vs. NCBI nr
Match: gi|645254539|ref|XP_008233085.1| (PREDICTED: cytochrome b5-like [Prunus mume])

HSP 1 Score: 188.0 bits (476), Expect = 1.1e-44
Identity = 91/138 (65.94%), Postives = 112/138 (81.16%), Query Frame = 1


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFE 60
           M SD +V+ FDEVAKHNH++DCW+IISGKVYDVTPFLE+HPGGDEVLLLA EKDATDDF 
Sbjct: 1   MASDSQVYHFDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKDATDDFN 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS SA EQME +Y+G VD ST+P+  +Y+L A    + T  S +SSG ++ LLQ L+
Sbjct: 61  DVGHSDSAKEQMENFYVGKVDTSTIPEQPNYKLPA----QTTPPSGQSSGFVVKLLQFLL 120

Query: 121 PLLIMGVAFYLQFYGKKQ 139
           PLLI+G AF LQ+YGKK+
Sbjct: 121 PLLILGAAFALQYYGKKK 134

The following BLAST results are available for this feature:

BLAST of Cp4.1LG08g00630 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CYB5D_ARATH	1.8e-34	50.00	Cytochrome B5 isoform D OS=Arabidopsis thaliana GN=CYTB5-D PE=1 SV=1	[more]
CYB5_BRAOB	4.0e-34	52.17	Cytochrome b5 OS=Brassica oleracea var. botrytis GN=CYB5 PE=1 SV=1	[more]
CYB5_TOBAC	5.3e-34	51.09	Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1	[more]
CYB5E_ARATH	6.9e-34	53.28	Cytochrome b5 isoform E OS=Arabidopsis thaliana GN=CYTB5-E PE=1 SV=2	[more]
CYB5S_TOBAC	4.5e-33	50.36	Cytochrome b5, seed isoform OS=Nicotiana tabacum PE=2 SV=1	[more]

BLAST of Cp4.1LG08g00630 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L720_CUCSA	1.0e-55	75.17	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G119260 PE=3 SV=1	[more]
M5X125_PRUPE	2.7e-45	66.67	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013234mg PE=3 SV=1	[more]
W9RDL9_9ROSA	8.2e-42	63.04	Cytochrome b5, seed isoform OS=Morus notabilis GN=L484_023457 PE=3 SV=1	[more]
I1NCP5_SOYBN	1.3e-39	58.70	Uncharacterized protein OS=Glycine max GN=GLYMA_19G258500 PE=3 SV=1	[more]
A0A0B2RSP9_GLYSO	1.3e-39	58.70	Cytochrome b5 isoform 1 OS=Glycine soja GN=glysoja_029033 PE=3 SV=1	[more]

BLAST of Cp4.1LG08g00630 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G48810.1	1.0e-35	50.00	cytochrome B5 isoform D	[more]
AT5G53560.1	3.9e-35	53.28	cytochrome B5 isoform E	[more]
AT2G32720.1	1.3e-33	48.53	cytochrome B5 isoform B	[more]
AT2G46650.1	6.4e-30	51.56	cytochrome B5 isoform C	[more]
AT1G26340.1	5.6e-26	58.54	cytochrome B5 isoform A	[more]

BLAST of Cp4.1LG08g00630 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449432012\|ref\|XP_004133794.1\|	1.4e-55	75.17	PREDICTED: cytochrome b5 [Cucumis sativus]	[more]
gi\|659074875\|ref\|XP_008437843.1\|	4.6e-54	75.86	PREDICTED: cytochrome b5-like [Cucumis melo]	[more]
gi\|596010726\|ref\|XP_007218583.1\|	3.9e-45	66.67	hypothetical protein PRUPE_ppa013234mg [Prunus persica]	[more]
gi\|657968938\|ref\|XP_008376183.1\|	6.7e-45	67.88	PREDICTED: cytochrome b5-like [Malus domestica]	[more]
gi\|645254539\|ref\|XP_008233085.1\|	1.1e-44	65.94	PREDICTED: cytochrome b5-like [Prunus mume]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding

Vocabulary: **INTERPRO**
Term	Definition
IPR018506	Cyt_B5_heme-BS
IPR001199	Cyt_B5-like_heme/steroid-bd

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0044710	single-organism metabolic process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0044444	cytoplasmic part
cellular_component	GO:0043231	intracellular membrane-bounded organelle
cellular_component	GO:0016020	membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG08g00630.1	Cp4.1LG08g00630.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 40..54 score: 1.4E-12coord: 55..62 score: 1.4E-12coord: 30..40 score: 1.4E-12coord: 68..80 score: 1.4
IPR001199	Cytochrome b5-like heme/steroid binding domain	GENE3D	G3DSA:3.10.120.10		coord: 5..87 score: 9.7
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 11..80 score: 5.2
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 score: 1.8
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROFILE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 27
IPR001199	Cytochrome b5-like heme/steroid binding domain	unknown	SSF55856	Cytochrome b5-like heme/steroid binding domain	coord: 4..89 score: 2.49
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43 scor
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..138 score: 4.0
None	No IPR available	PANTHER	PTHR19359:SF22	CYTOCHROME B5 ISOFORM A	coord: 1..138 score: 4.0

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cp4.1LG08g00630	Cucsa.259540	Cucumber (Gy14) v1	cgycpeB0687
Cp4.1LG08g00630	CmaCh14G021300	Cucurbita maxima (Rimu)	cmacpeB292
Cp4.1LG08g00630	CmaCh06G009870	Cucurbita maxima (Rimu)	cmacpeB848
Cp4.1LG08g00630	CmoCh06G010160	Cucurbita moschata (Rifu)	cmocpeB796
Cp4.1LG08g00630	CmoCh14G021930	Cucurbita moschata (Rifu)	cmocpeB254
Cp4.1LG08g00630	Cla021123	Watermelon (97103) v1	cpewmB845
Cp4.1LG08g00630	Csa3G119260	Cucumber (Chinese Long) v2	cpecuB862
Cp4.1LG08g00630	MELO3C005994	Melon (DHL92) v3.5.1	cpemeB815
Cp4.1LG08g00630	ClCG05G000550	Watermelon (Charleston Gray)	cpewcgB787
Cp4.1LG08g00630	CSPI03G07620	Wild cucumber (PI 183967)	cpecpiB864
Cp4.1LG08g00630	Lsi05G021600	Bottle gourd (USVL1VR-Ls)	cpelsiB714
Cp4.1LG08g00630	MELO3C005994.2	Melon (DHL92) v3.6.1	cpemedB951
Cp4.1LG08g00630	CsaV3_5G000250	Cucumber (Chinese Long) v3	cpecucB1071
Cp4.1LG08g00630	CsaV3_3G007770	Cucumber (Chinese Long) v3	cpecucB1058
Cp4.1LG08g00630	Bhi01G000393	Wax gourd	cpewgoB1105
Cp4.1LG08g00630	Bhi12G000587	Wax gourd	cpewgoB1098
Cp4.1LG08g00630	CsGy3G007780	Cucumber (Gy14) v2	cgybcpeB454
Cp4.1LG08g00630	Carg25714	Silver-seed gourd	carcpeB0180
Cp4.1LG08g00630	Carg20915	Silver-seed gourd	carcpeB1380

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Cp4.1LG08g00630	Cp4.1LG03g16000	Cucurbita pepo (Zucchini)	cpecpeB482

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG08g00630	Cucurbita maxima (Rimu)	cmacpeB014
Cp4.1LG08g00630	Cucurbita maxima (Rimu)	cmacpeB015
Cp4.1LG08g00630	Melon (DHL92) v3.6.1	cpemedB929