Carg20915 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg20915
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	cytochrome b5-like
Location	Cucurbita_argyrosperma_scaffold_141 : 331216 .. 333703 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTGGGCGTTTTTTGATCTCCACCAAGCCGGCCAGATAAAAGCCTTGTAAAATGGCTGGTTGTCATTACCGGAAGAAATCGTGCTGATTTGGGTCTTTTCTCCAATTTCCCTTCAACGCTTAATCCGTAAGAAATTCATCTTCTTCCTCATTGCCTCTGTGTTTGAATCGTCTGATTTTCTAATTTAATCGATTGCTTCAGTGTGGATTGAGCTTGACTTTTGTTTGCATTTCAGCTGAAATGGATTCGGATCCTAAGGTTTTTGTCTTCGACGAAGTGGCTAAACACAATCACCAGCGGGATTGTTGGCTTATAATCTCTGGAAAGGTCTGATATCATAATCTCTCTTCATTTTTAATCTCCATATCGATATTTTGTTTGCTCTGAACTCAATCGTAACTGCTACTGGAACTCTAGACTTATAGAACAGTAGATAGAGGGATGGGTGATTAAGAAACTGATGATTGTGTATTCTATTATGATTATTGTTCTTTGATATCTCCGCGAGTTGGAACTCGCATCTGACTTCTTACCGTTTGACTTTATGACATTTGATTAGGTTTAACATCGTAGGGAGATGACCCATATGAGATTTGAACTTATTACTAGTAGATATGGTCTGTTTTAGCCCATTATGTATCGCCATTAGCTTCATAGTTTTAAAGCGCGTCTACTGAGAAGAGGTTTCCACACCCTTATAAGAATGCTTCGTTCTCCTCTCCAACTGTGGTGAGATCTCACAATCCACCTCCCTTGAGGGCCTAGCGTTCTTGCTGGCATACCGCCCGGTGTCTAGCTCTAATACCATTTGTAACATCCCAAGCCCACCGCTAGCTGATATTGTTTGTTTTAGCCCATTACGTATCACCGTCTGCCTCACAATTTTAGAACGCGTCTACTAGGGAGAGGTTTTCACACCCTTATGAGGAACGATTTGTTCCCCTCTTCAACTGAGGTGGGATCTCACAATCCACCTCCCTTTGGGGCCAGCGTCCTCGCTGGGACACTACCCAGTGTCTGGCTCTGATACCATTTGTACATCCCAAGCCTACCGCTAGCAGATATTGTCCTTTTTGAGCTTTTCCTTTTGGACTTCCTCTCAAGGTTTTTAAAACGCGTTTGCTAGGGAGAGGTTTCCTATAAAGAATGTTTCATTCTCCTTCCCAACCGATCTGAGATCTCACAAGAAGGGTGAGAGACAGGACGTATGATCTTTGTAGAGTACTTGAACTAGGGGTAAATTGTTTATGGTAGATAGGAAACTGAAAGCACAGAAAAGTCTGCTGCAACTCTAGATTTCTATTAGTTAGGACTATGGAGACTGATATCCTTTATTTCTGGAGCAACTAATATAGATATTGTACTAACAGACAGGAACTTATTCCTCTAAGCTCCTATTTCGTGGAAGCAGAATTTAAGGCCTAGAAAGGCTTAGGCTTAGTAGAGAGTAATACTTTGCGGAATCCTTGTTTTAAATTTTAAGCATGGAACGTGAGGATGTTTAGTGGGGATACATCGTAAGCCAAGTTTTATCAGAGCACTTGGTATCAAATTTTTTGGATTCGATCTAACAACCTTGTTAAAGAACGAGATCTCTAGATCTAAATTATCTAAAAACTCCAAAAACGATAAGGAATCAACTTAAGGGAAAGTTTTGGAACAGATTAACTAGATGATCAAGATCAAGAGTAAAGAAAACTCGTTTCTAAACTCGTGATTCGAATCACTCCACAAGATAACTTCCCTATCCCTTATATAGTCATCGTGTTTCACTGCTCTGCCACGAAGTAATTATTGTGCGCTCCGATGTTAGGTATATGATGTGACCTCTTTTCTGGAAGAACATCCTGGAGGCGATGAAGTGTTATTATTAGCTGCTGGTATGTTTCAGCTCAATCTCGATTGGTTGTTACAAATGATTACACCAAGCTATGAATGATTGGAAAAACAATGTTGAGTTTATGCAGAGAAAGATGCAACGGATGATTTTGAAAATGTGGGTCACAGTCTATCTGCAATAGAGCAAATGGAAAAGTATTACATTGGCAATGTTGACATGTCTACGGTCCCAAAGCCGATCGATTATCGACTAGCCGCATCCCAATCGGTAAAAGCAACGACGTCGTCGGCTGAATCTTCAGGATCCTTGCTAACACTACTGCAAATTCTGGTTCCCCTGCTGATTATGGGCGTAGCAATTTATCTGCAATTCTATGGCAAAAAGCAGTAATCCTTAGCTTCCTACCAAAGATGCCACGAGATTTTCAATTACTCACTTTATGTTGAAGACATTGATATTACGTAGGTTTCTCTGCCATTGCCTCTTGCTAATTTCTGAGTAGATAATTTAAAAACAGTTCATTTGTGTCTTGTTCATTCATTATCCTGTTTTCCTTTGTCTTTTGTTCTAATATATTCAACTTTCTGTGCTTGATTTAACACCATTGACTCACCAGACTCGCCAGATATATGAAGTTGAATGTAGATC

mRNA sequence

ATTGGGCGTTTTTTGATCTCCACCAAGCCGGCCAGATAAAAGCCTTGTAAAATGGCTGGTTGTCATTACCGGAAGAAATCGTGCTGATTTGGGTCTTTTCTCCAATTTCCCTTCAACGCTTAATCCCTGAAATGGATTCGGATCCTAAGGTTTTTGTCTTCGACGAAGTGGCTAAACACAATCACCAGCGGGATTGTTGGCTTATAATCTCTGGAAAGGTATATGATGTGACCTCTTTTCTGGAAGAACATCCTGGAGGCGATGAAGTGTTATTATTAGCTGCTGAGAAAGATGCAACGGATGATTTTGAAAATGTGGGTCACAGTCTATCTGCAATAGAGCAAATGGAAAAGTATTACATTGGCAATGTTGACATGTCTACGGTCCCAAAGCCGATCGATTATCGACTAGCCGCATCCCAATCGGTAAAAGCAACGACGTCGTCGGCTGAATCTTCAGGATCCTTGCTAACACTACTGCAAATTCTGGTTCCCCTGCTGATTATGGGCGTAGCAATTTATCTGCAATTCTATGGCAAAAAGCAGTAATCCTTAGCTTCCTACCAAAGATGCCACGAGATTTTCAATTACTCACTTTATGTTGAAGACATTGATATTACGTAGGTTTCTCTGCCATTGCCTCTTGCTAATTTCTGAGTAGATAATTTAAAAACAGTTCATTTGTGTCTTGTTCATTCATTATCCTGTTTTCCTTTGTCTTTTGTTCTAATATATTCAACTTTCTGTGCTTGATTTAACACCATTGACTCACCAGACTCGCCAGATATATGAAGTTGAATGTAGATC

Coding sequence (CDS)

ATGGATTCGGATCCTAAGGTTTTTGTCTTCGACGAAGTGGCTAAACACAATCACCAGCGGGATTGTTGGCTTATAATCTCTGGAAAGGTATATGATGTGACCTCTTTTCTGGAAGAACATCCTGGAGGCGATGAAGTGTTATTATTAGCTGCTGAGAAAGATGCAACGGATGATTTTGAAAATGTGGGTCACAGTCTATCTGCAATAGAGCAAATGGAAAAGTATTACATTGGCAATGTTGACATGTCTACGGTCCCAAAGCCGATCGATTATCGACTAGCCGCATCCCAATCGGTAAAAGCAACGACGTCGTCGGCTGAATCTTCAGGATCCTTGCTAACACTACTGCAAATTCTGGTTCCCCTGCTGATTATGGGCGTAGCAATTTATCTGCAATTCTATGGCAAAAAGCAGTAA

Protein sequence

MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFENVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILVPLLIMGVAIYLQFYGKKQ

Homology

[Close]

BLAST of Carg20915 vs. NCBI nr
Match: XP_022931217.1 (cytochrome B5-like [Cucurbita moschata] >XP_022974834.1 cytochrome B5-like [Cucurbita maxima] >XP_022974835.1 cytochrome B5-like [Cucurbita maxima])

HSP 1 Score: 272.7 bits (696), Expect = 6.8e-70
Identity = 137/138 (99.28%), Postives = 137/138 (99.28%), Query Frame = 0


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE 60
           MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE
Sbjct: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV
Sbjct: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120

Query: 121 PLLIMGVAIYLQFYGKKQ 139
           PLLIMGVA YLQFYGKKQ
Sbjct: 121 PLLIMGVAFYLQFYGKKQ 138

[Close]

BLAST of Carg20915 vs. NCBI nr
Match: XP_022975276.1 (cytochrome B5-like [Cucurbita maxima])

HSP 1 Score: 272.3 bits (695), Expect = 8.9e-70
Identity = 136/138 (98.55%), Postives = 137/138 (99.28%), Query Frame = 0


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE 60
           MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE
Sbjct: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           NVGHSLSAIEQMEKYYIGNVDMST+PKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV
Sbjct: 61  NVGHSLSAIEQMEKYYIGNVDMSTIPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120

Query: 121 PLLIMGVAIYLQFYGKKQ 139
           PLLIMGVA YLQFYGKKQ
Sbjct: 121 PLLIMGVAFYLQFYGKKQ 138

[Close]

BLAST of Carg20915 vs. NCBI nr
Match: XP_023540248.1 (cytochrome b5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 270.8 bits (691), Expect = 2.6e-69
Identity = 136/138 (98.55%), Postives = 136/138 (98.55%), Query Frame = 0


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE 60
           MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVT FLEEHPGGDEVLLLAAEKDATDDFE
Sbjct: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV
Sbjct: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120

Query: 121 PLLIMGVAIYLQFYGKKQ 139
           PLLIMGVA YLQFYGKKQ
Sbjct: 121 PLLIMGVAFYLQFYGKKQ 138

[Close]

BLAST of Carg20915 vs. NCBI nr
Match: XP_022147466.1 (cytochrome b5-like [Momordica charantia])

HSP 1 Score: 233.0 bits (593), Expect = 6.0e-58
Identity = 114/138 (82.61%), Postives = 125/138 (90.58%), Query Frame = 0


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE 60
           M SDPKVF FDEVAKHNHQRDCWLIISGKVYDVT FLEEHPGGDEVL+LA+EKDATDDFE
Sbjct: 1   MGSDPKVFAFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLILASEKDATDDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           NVGHSLSAI+QME+YY+GNVDMSTVPKPIDYR  ASQS   T +SA+SSGS L LLQ L+
Sbjct: 61  NVGHSLSAIDQMEQYYVGNVDMSTVPKPIDYRPPASQSA-TTLASAQSSGSSLKLLQFLI 120

Query: 121 PLLIMGVAIYLQFYGKKQ 139
           PLL++GVA Y+QFYGKKQ
Sbjct: 121 PLLLIGVAFYMQFYGKKQ 137

[Close]

BLAST of Carg20915 vs. NCBI nr
Match: XP_004133794.1 (PREDICTED: cytochrome b5 [Cucumis sativus] >XP_011650661.1 PREDICTED: cytochrome b5 [Cucumis sativus] >KGN56392.1 hypothetical protein Csa_3G119260 [Cucumis sativus])

HSP 1 Score: 214.5 bits (545), Expect = 2.2e-52
Identity = 105/145 (72.41%), Postives = 120/145 (82.76%), Query Frame = 0


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE 60
           M SDPK+FVFDEVAKHNHQ DCWLIISGKVYDVT FLE+HPGGDEVLLLA EKDAT+DFE
Sbjct: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTS-------SAESSGSLL 120
            V HSL A E+MEKYYIGN+DMST+PKP D+R  AS+S  A T         A+SSGSL+
Sbjct: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEXXXXXXXXAQSSGSLI 120

Query: 121 TLLQILVPLLIMGVAIYLQFYGKKQ 139
            +LQ+L+PLLI+GVA YLQ+YGKKQ
Sbjct: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 145

[Close]

BLAST of Carg20915 vs. TAIR10
Match: AT5G48810.1 (cytochrome B5 isoform D)

HSP 1 Score: 144.1 bits (362), Expect = 6.6e-35
Identity = 67/136 (49.26%), Postives = 97/136 (71.32%), Query Frame = 0


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE 60
           M  D KVF   EV++H+  +DCW++I GKVYDVT FL++HPGGDEV+L +  KDATDDFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS +A   +++YY+G++D +TV  P+  +     S KA  +  +SS  ++ LLQ LV
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATV--PVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLV 120

Query: 121 PLLIMGVAIYLQFYGK 137
           PLLI+G+A  +++Y K
Sbjct: 121 PLLILGLAFGIRYYTK 134

[Close]

BLAST of Carg20915 vs. TAIR10
Match: AT5G53560.1 (cytochrome B5 isoform E)

HSP 1 Score: 142.1 bits (357), Expect = 2.5e-34
Identity = 72/137 (52.55%), Postives = 98/137 (71.53%), Query Frame = 0


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE 60
           M SD KV  F+EV+KHN  +DCWLIISGKVYDVT F+++HPGGDEVLL +  KDAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS +A + M+KY+IG +D S+VP    Y +A  Q       + E    ++ +LQ LV
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTY-VAPQQPAYNQDKTPE---FIIKILQFLV 120

Query: 121 PLLIMGVAIYLQFYGKK 138
           P+LI+G+A+ ++ Y KK
Sbjct: 121 PILILGLALVVRHYTKK 133

[Close]

BLAST of Carg20915 vs. TAIR10
Match: AT2G32720.1 (cytochrome B5 isoform B)

HSP 1 Score: 141.0 bits (354), Expect = 5.6e-34
Identity = 66/136 (48.53%), Postives = 96/136 (70.59%), Query Frame = 0


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE 60
           M  + K+F   EV++HN   DCW++I+GKVY+VT FLE+HPGGD+VLL +  KDATDDFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS SA E ME+YY+G +D +T+PK + Y    +   +   +  ++S  ++ LLQ LV
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKY----TPPKQPHYNQDKTSEFIIKLLQFLV 120

Query: 121 PLLIMGVAIYLQFYGK 137
           PL I+G+A+ ++ Y K
Sbjct: 121 PLAILGLAVGIRIYTK 132

[Close]

BLAST of Carg20915 vs. TAIR10
Match: AT2G46650.1 (cytochrome B5 isoform C)

HSP 1 Score: 125.6 bits (314), Expect = 2.4e-29
Identity = 65/128 (50.78%), Postives = 84/128 (65.62%), Query Frame = 0


Query: 10  FDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFENVGHSLSAI 69
           F +VAKH  + DCW++I GKVYD+++F++EHPGGD VLL    KDA+ DFE+V HS  A 
Sbjct: 7   FHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSKDAK 66

Query: 70  EQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILVPLLIMGVAI 129
           E M+KY IG+VD STVP    Y     +   A  ++ E SG    LL  L+PLLI+GVA 
Sbjct: 67  ELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGK--KLLIYLIPLLILGVAF 126

Query: 130 YLQFYGKK 138
            L+FY  K
Sbjct: 127 ALRFYNNK 132

[Close]

BLAST of Carg20915 vs. TAIR10
Match: AT1G26340.1 (cytochrome B5 isoform A)

HSP 1 Score: 115.9 bits (289), Expect = 1.9e-26
Identity = 49/82 (59.76%), Postives = 65/82 (79.27%), Query Frame = 0


Query: 6  KVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFENVGHS 65
          K++  +E A HN Q DCW++I GKVYDV+S+++EHPGGD+VLL  A KDATDDFE+ GHS
Sbjct: 6  KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 66 LSAIEQMEKYYIGNVDMSTVPK 88
            A E MEKY+IG +D S++P+
Sbjct: 66 KDARELMEKYFIGELDESSLPE 87

[Close]

BLAST of Carg20915 vs. Swiss-Prot
Match: sp|Q9ZWT2|CYB5D_ARATH (Cytochrome B5 isoform D OS=Arabidopsis thaliana OX=3702 GN=CYTB5-D PE=1 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 1.2e-33
Identity = 67/136 (49.26%), Postives = 97/136 (71.32%), Query Frame = 0


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE 60
           M  D KVF   EV++H+  +DCW++I GKVYDVT FL++HPGGDEV+L +  KDATDDFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS +A   +++YY+G++D +TV  P+  +     S KA  +  +SS  ++ LLQ LV
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATV--PVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLV 120

Query: 121 PLLIMGVAIYLQFYGK 137
           PLLI+G+A  +++Y K
Sbjct: 121 PLLILGLAFGIRYYTK 134

[Close]

BLAST of Carg20915 vs. Swiss-Prot
Match: sp|P40934|CYB5_BRAOB (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 2.7e-33
Identity = 71/138 (51.45%), Postives = 99/138 (71.74%), Query Frame = 0


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE 60
           M S+ KV  F+EV++HN  +DCWLIISGKVYDVT F+++HPGGDEVLL +  KDAT+DFE
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS +A + MEKYYIG +D STVP    Y       V+   +  ++   ++ +LQ LV
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVPATRTY----VAPVQPAYNQDKTPEFMIKILQFLV 120

Query: 121 PLLIMGVAIYLQFYGKKQ 139
           P+LI+G+A+ ++ Y KK+
Sbjct: 121 PILILGLALVVRQYTKKE 134

[Close]

BLAST of Carg20915 vs. Swiss-Prot
Match: sp|P49098|CYB5_TOBAC (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 3.5e-33
Identity = 69/137 (50.36%), Postives = 98/137 (71.53%), Query Frame = 0


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE 60
           M  + KVF   EV++HN+ +DCWL+ISGKVYDVT FL++HPGGDEVLL A  KDATDDFE
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS SA   +++YY+G++D +T+P    Y    +   +   +  ++S  ++ LLQ LV
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKY----TPPNQPHYNQDKTSEFVVKLLQFLV 120

Query: 121 PLLIMGVAIYLQFYGKK 138
           PL+I+GVA  ++FY K+
Sbjct: 121 PLIILGVAFGIRFYTKQ 133

[Close]

BLAST of Carg20915 vs. Swiss-Prot
Match: sp|Q42342|CYB5E_ARATH (Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 142.1 bits (357), Expect = 4.5e-33
Identity = 72/137 (52.55%), Postives = 98/137 (71.53%), Query Frame = 0


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE 60
           M SD KV  F+EV+KHN  +DCWLIISGKVYDVT F+++HPGGDEVLL +  KDAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS +A + M+KY+IG +D S+VP    Y +A  Q       + E    ++ +LQ LV
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTY-VAPQQPAYNQDKTPE---FIIKILQFLV 120

Query: 121 PLLIMGVAIYLQFYGKK 138
           P+LI+G+A+ ++ Y KK
Sbjct: 121 PILILGLALVVRHYTKK 133

[Close]

BLAST of Carg20915 vs. Swiss-Prot
Match: sp|O48845|CYB5B_ARATH (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 1.0e-32
Identity = 66/136 (48.53%), Postives = 96/136 (70.59%), Query Frame = 0


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE 60
           M  + K+F   EV++HN   DCW++I+GKVY+VT FLE+HPGGD+VLL +  KDATDDFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS SA E ME+YY+G +D +T+PK + Y    +   +   +  ++S  ++ LLQ LV
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKY----TPPKQPHYNQDKTSEFIIKLLQFLV 120

Query: 121 PLLIMGVAIYLQFYGK 137
           PL I+G+A+ ++ Y K
Sbjct: 121 PLAILGLAVGIRIYTK 132

[Close]

BLAST of Carg20915 vs. TrEMBL
Match: tr|A0A0A0L720|A0A0A0L720_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G119260 PE=3 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.5e-52
Identity = 105/145 (72.41%), Postives = 120/145 (82.76%), Query Frame = 0


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE 60
           M SDPK+FVFDEVAKHNHQ DCWLIISGKVYDVT FLE+HPGGDEVLLLA EKDAT+DFE
Sbjct: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTS-------SAESSGSLL 120
            V HSL A E+MEKYYIGN+DMST+PKP D+R  AS+S  A T         A+SSGSL+
Sbjct: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEXXXXXXXXAQSSGSLI 120

Query: 121 TLLQILVPLLIMGVAIYLQFYGKKQ 139
            +LQ+L+PLLI+GVA YLQ+YGKKQ
Sbjct: 121 KVLQVLIPLLIIGVAFYLQYYGKKQ 145

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BLAST of Carg20915 vs. TrEMBL
Match: tr|M5X125|M5X125_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_2G194100 PE=3 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 2.8e-43
Identity = 90/138 (65.22%), Postives = 110/138 (79.71%), Query Frame = 0


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE 60
           M SD +V+ FDEVAKHNH++DCW+IISGKVYDVT FLE+HPGGDEVLLLA EKDATDDF 
Sbjct: 1   MASDSQVYHFDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKDATDDFN 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS SA EQMEK+Y+G VD ST+P+   Y+L A    + T  S +SSG ++ LLQ L+
Sbjct: 61  DVGHSDSAKEQMEKFYVGKVDTSTIPEQPSYKLPA----QTTPPSGQSSGFVVKLLQFLL 120

Query: 121 PLLIMGVAIYLQFYGKKQ 139
           PLLI+G A  LQ+YGKK+
Sbjct: 121 PLLILGAAFALQYYGKKK 134

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BLAST of Carg20915 vs. TrEMBL
Match: tr|A0A2P5FIE4|A0A2P5FIE4_9ROSA (Nitrate reductase NADH dependent OS=Trema orientalis OX=63057 GN=TorRG33x02_067620 PE=3 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 4.4e-41
Identity = 87/138 (63.04%), Postives = 107/138 (77.54%), Query Frame = 0


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE 60
           M S  KVF FDEVA+HNH++DCW+IISGK YDVT FLEEHPGGDEVLLLAAEKDATDDFE
Sbjct: 1   MASGSKVFTFDEVAQHNHKKDCWIIISGKAYDVTEFLEEHPGGDEVLLLAAEKDATDDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS  A E ++KY +G VD+ST+P+  +Y+     S      S +SSGS + +LQ L+
Sbjct: 61  DVGHSDDAKELLKKYCVGEVDLSTLPEKPNYKPPTQSS-----QSDQSSGSAVKILQFLL 120

Query: 121 PLLIMGVAIYLQFYGKKQ 139
           PLLI+G+A  LQFYGKK+
Sbjct: 121 PLLILGIAFALQFYGKKK 133

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BLAST of Carg20915 vs. TrEMBL
Match: tr|A0A2P5DQA7|A0A2P5DQA7_PARAD (Nitrate reductase NADH dependent OS=Parasponia andersonii OX=3476 GN=PanWU01x14_040920 PE=3 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 4.4e-41
Identity = 86/138 (62.32%), Postives = 109/138 (78.99%), Query Frame = 0


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE 60
           M S  KVF FDEVA+HNH++DCW+IISGK YDVT FLEEHPGGDEVLLLAAEKDATDDFE
Sbjct: 1   MASGSKVFTFDEVAQHNHKKDCWIIISGKAYDVTEFLEEHPGGDEVLLLAAEKDATDDFE 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILV 120
           +VGHS  A E ++KY +G VD+ST+P+  +Y+        +++ S +SSGS + +LQ L+
Sbjct: 61  DVGHSDDAKELLKKYCVGEVDLSTLPEKPNYK----PPTHSSSQSDQSSGSAVKILQFLL 120

Query: 121 PLLIMGVAIYLQFYGKKQ 139
           PLLI+G+A  LQFYGKK+
Sbjct: 121 PLLILGIAFALQFYGKKK 134

[Close]

BLAST of Carg20915 vs. TrEMBL
Match: tr|A0A2P6SIR0|A0A2P6SIR0_ROSCH (Putative cytochrome b5-like heme/steroid binding domain-containing protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr1g0360711 PE=3 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 2.2e-40
Identity = 87/139 (62.59%), Postives = 110/139 (79.14%), Query Frame = 0


Query: 1   MDSDPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFE 60
           M SD KV+ FDEVA+H +++DCW+I+SGKVYDVT FLEEHPGGDEVLLLAA++DATDDF 
Sbjct: 1   MASDSKVYHFDEVAQHKYKKDCWIIVSGKVYDVTPFLEEHPGGDEVLLLAADRDATDDFN 60

Query: 61  NVGHSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSA-ESSGSLLTLLQIL 120
           +VGHS SA E MEKYY+G VD ST+P+  +Y+L A    +AT   A +SSG ++ LLQ L
Sbjct: 61  DVGHSDSAKEMMEKYYVGEVDTSTIPEQPNYKLPA----QATAPQADQSSGFVVKLLQFL 120

Query: 121 VPLLIMGVAIYLQFYGKKQ 139
           +PLLI+G A  LQ+Y KK+
Sbjct: 121 LPLLILGAAFALQYYSKKK 135

The following BLAST results are available for this feature:

BLAST of Carg20915 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022931217.1	6.8e-70	99.28	cytochrome B5-like [Cucurbita moschata] >XP_022974834.1 cytochrome B5-like [Cucu...	[more]
XP_022975276.1	8.9e-70	98.55	cytochrome B5-like [Cucurbita maxima]	[more]
XP_023540248.1	2.6e-69	98.55	cytochrome b5-like [Cucurbita pepo subsp. pepo]	[more]
XP_022147466.1	6.0e-58	82.61	cytochrome b5-like [Momordica charantia]	[more]
XP_004133794.1	2.2e-52	72.41	PREDICTED: cytochrome b5 [Cucumis sativus] >XP_011650661.1 PREDICTED: cytochrome...	[more]

BLAST of Carg20915 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G48810.1	6.6e-35	49.26	cytochrome B5 isoform D	[more]
AT5G53560.1	2.5e-34	52.55	cytochrome B5 isoform E	[more]
AT2G32720.1	5.6e-34	48.53	cytochrome B5 isoform B	[more]
AT2G46650.1	2.4e-29	50.78	cytochrome B5 isoform C	[more]
AT1G26340.1	1.9e-26	59.76	cytochrome B5 isoform A	[more]

BLAST of Carg20915 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9ZWT2\|CYB5D_ARATH	1.2e-33	49.26	Cytochrome B5 isoform D OS=Arabidopsis thaliana OX=3702 GN=CYTB5-D PE=1 SV=1	[more]
sp\|P40934\|CYB5_BRAOB	2.7e-33	51.45	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]
sp\|P49098\|CYB5_TOBAC	3.5e-33	50.36	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
sp\|Q42342\|CYB5E_ARATH	4.5e-33	52.55	Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2	[more]
sp\|O48845\|CYB5B_ARATH	1.0e-32	48.53	Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1	[more]

BLAST of Carg20915 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0L720\|A0A0A0L720_CUCSA	1.5e-52	72.41	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G119260 PE=3 SV=1	[more]
tr\|M5X125\|M5X125_PRUPE	2.8e-43	65.22	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_2G194100 PE=3 SV=1	[more]
tr\|A0A2P5FIE4\|A0A2P5FIE4_9ROSA	4.4e-41	63.04	Nitrate reductase NADH dependent OS=Trema orientalis OX=63057 GN=TorRG33x02_0676...	[more]
tr\|A0A2P5DQA7\|A0A2P5DQA7_PARAD	4.4e-41	62.32	Nitrate reductase NADH dependent OS=Parasponia andersonii OX=3476 GN=PanWU01x14_...	[more]
tr\|A0A2P6SIR0\|A0A2P6SIR0_ROSCH	2.2e-40	62.59	Putative cytochrome b5-like heme/steroid binding domain-containing protein OS=Ro...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding

Vocabulary: **INTERPRO**
Term	Definition
IPR018506	Cyt_B5_heme-BS
IPR036400	Cyt_B5-like_heme/steroid_sf
IPR001199	Cyt_B5-like_heme/steroid-bd

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg20915-RA	Carg20915-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 40..54 score: 52.32 coord: 55..62 score: 43.94 coord: 30..40 score: 65.56 coord: 68..80 score: 35.08
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 e-value: 3.5E-28 score: 109.6
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 11..80 e-value: 3.1E-24 score: 84.8
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 27.838
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	G3DSA:3.10.120.10		coord: 1..87 e-value: 6.9E-33 score: 114.3
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	SSF55856	Cytochrome b5-like heme/steroid binding domain	coord: 4..89
None	No IPR available	PANTHER	PTHR19359:SF43	CYTOCHROME B5 ISOFORM C	coord: 1..137
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..137
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg20915	Cla021123	Watermelon (97103) v1	carwmB0992
Carg20915	Csa3G119260	Cucumber (Chinese Long) v2	carcuB1007
Carg20915	MELO3C005994	Melon (DHL92) v3.5.1	carmeB0968
Carg20915	CSPI03G07620	Wild cucumber (PI 183967)	carcpiB1031
Carg20915	Cucsa.259540	Cucumber (Gy14) v1	carcgyB1110
Carg20915	CmaCh14G021300	Cucurbita maxima (Rimu)	carcmaB1373
Carg20915	CmoCh06G010160	Cucurbita moschata (Rifu)	carcmoB1332
Carg20915	CmoCh14G021930	Cucurbita moschata (Rifu)	carcmoB1330
Carg20915	Cp4.1LG08g00630	Cucurbita pepo (Zucchini)	carcpeB1380
Carg20915	Cp4.1LG03g16000	Cucurbita pepo (Zucchini)	carcpeB1379
Carg20915	CsGy3G007780	Cucumber (Gy14) v2	carcgybB0980
Carg20915	MELO3C005994.2	Melon (DHL92) v3.6.1	carmedB0978
Carg20915	CsaV3_3G007770	Cucumber (Chinese Long) v3	carcucB1037
Carg20915	Cla97C01G000230	Watermelon (97103) v2	carwmbB1044
Carg20915	Bhi01G000393	Wax gourd	carwgoB1162

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg20915	Carg25714	Silver-seed gourd	carcarB146

The following block(s) are covering this gene:


Gene	Organism	Block
Carg20915	Cucurbita maxima (Rimu)	carcmaB1371
Carg20915	Cucurbita maxima (Rimu)	carcmaB1376
Carg20915	Bottle gourd (USVL1VR-Ls)	carlsiB907
Carg20915	Watermelon (Charleston Gray)	carwcgB0968
Carg20915	Watermelon (97103) v2	carwmbB1047