Carg25714 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg25714
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	cytochrome b5-like
Location	Cucurbita_argyrosperma_scaffold_341 : 105914 .. 107198 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCGGATCCTAAGGTTTTCGTCTTCGATGAAGTGGCTAAACACAATAACCAGCAGGATTGTTGGGTTATAATCTCTGGAAAGGTCTGATTTAACAATCTCTCTTCTAATCTTCATTTTGTGAAATTGTTTGTTCAGAACTCAAATCGTATTTGCCAATGGAACAGTAGACTTAAATGAGATTAAGCCTATTAGTTTCTTGATCTTTCTCCTAACAATCTTTGAACTCATTACTCGAGAAATGACAGGAACTTGTTCTTCATCTTTTCTTCTGATGTGAAATCAGTTGTTGAGGATTAAAACATAAATCCTATGCATGTGTGAGACGTATTTCGTGGAATTGTTCAAGAGGAAAAACTCTGGTATTGTAGTTTGCAATGAATCTAAGCTCCAACTTTCATCAAAGAAGAATTTTAGGCCTCAAACTTCTTTTACGTTTAACAACATAGGAAGGAGATTTGATTTCCTCACTTAATCACATTTTCTCTCCCTTTAAACACCGATTTGGCCTCAAACATTCCTTTTCGGTCCATTTGCTCGAAATTTACAATTTAAACAACCATGCTCGTGAAATTCACTAATTCAACCACAAAACCGTGCAAAATCTTAGTCCTGGGAATAACAGATTATGTGTACTATCAGTTATCTCTGTATTCTTCTCCTAGATATACCTAAAATAAGACACCTTCAATTTGGGTGATAAGATTTGTATTTTTTCTATCCTCTTCCTTTATATTGTTGTTGTGTTTCGTTGCCTTTCCCCAAAGTAGTTTTTGTGATCCATGTTAGGTGTATGATGTCACCTCCCTTCTAGAAGATCATCCTGGAGGTGATGAAGTCTTGCTGTTAGCTACTGGTAAGTCCAACTTGATTACTTGTTTCTATTTGAAGATATCCAACAAAAATGATCACACCACGTTATGAATGATTGGAGAAACAATCTTGAATGTGTGCAGAAAGAGATGCCACTGATGATTTCGAAGAAGTGGGTCACAGTTTAACTGCGTCGGAGCAAATGGAAGAATATTATGTTGGTAATGTGGACATGTCTACCCTTCCAAAGGCAGTTGCCAATCGACCACCAGCACCCCAATCGGGGACAGCGACAACAGCAGCAGCAAAACCAACGGCAGCAGCAGAACCAAAAGCAGCAGCACCAACAACAACAACAACGTCGTCAGCTGAATCTCCAGGATCTTTGAAAAAAGTATTGCAAGTTCTGATTCCCCTGCTGATTTTGGGTATTGCATATTATATGCAATACTATGGCAAGAAACAGTAA

mRNA sequence

ATGGCTTCGGATCCTAAGGTTTTCGTCTTCGATGAAGTGGCTAAACACAATAACCAGCAGGATTGTTGGGTTATAATCTCTGGAAAGGTGTATGATGTCACCTCCCTTCTAGAAGATCATCCTGGAGGTGATGAAGTCTTGCTGTTAGCTACTGAAAGAGATGCCACTGATGATTTCGAAGAAGTGGGTCACAGTTTAACTGCGTCGGAGCAAATGGAAGAATATTATGTTGGTAATGTGGACATGTCTACCCTTCCAAAGGCAGTTGCCAATCGACCACCAGCACCCCAATCGGGGACAGCGACAACAGCAGCAGCAAAACCAACGGCAGCAGCAGAACCAAAAGCAGCAGCACCAACAACAACAACAACGTCGTCAGCTGAATCTCCAGGATCTTTGAAAAAAGTATTGCAAGTTCTGATTCCCCTGCTGATTTTGGGTATTGCATATTATATGCAATACTATGGCAAGAAACAGTAA

Coding sequence (CDS)

ATGGCTTCGGATCCTAAGGTTTTCGTCTTCGATGAAGTGGCTAAACACAATAACCAGCAGGATTGTTGGGTTATAATCTCTGGAAAGGTGTATGATGTCACCTCCCTTCTAGAAGATCATCCTGGAGGTGATGAAGTCTTGCTGTTAGCTACTGAAAGAGATGCCACTGATGATTTCGAAGAAGTGGGTCACAGTTTAACTGCGTCGGAGCAAATGGAAGAATATTATGTTGGTAATGTGGACATGTCTACCCTTCCAAAGGCAGTTGCCAATCGACCACCAGCACCCCAATCGGGGACAGCGACAACAGCAGCAGCAAAACCAACGGCAGCAGCAGAACCAAAAGCAGCAGCACCAACAACAACAACAACGTCGTCAGCTGAATCTCCAGGATCTTTGAAAAAAGTATTGCAAGTTCTGATTCCCCTGCTGATTTTGGGTATTGCATATTATATGCAATACTATGGCAAGAAACAGTAA

Protein sequence

MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFEEVGHSLTASEQMEEYYVGNVDMSTLPKAVANRPPAPQSGTATTAAAKPTAAAEPKAAAPTTTTTSSAESPGSLKKVLQVLIPLLILGIAYYMQYYGKKQ

Homology

[Close]

BLAST of Carg25714 vs. NCBI nr
Match: XP_022924404.1 (cytochrome b5-like [Cucurbita moschata])

HSP 1 Score: 249.2 bits (635), Expect = 9.3e-63
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFE 60
           MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFE
Sbjct: 1   MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFE 60

Query: 61  EVGHSLTASEQMEEYYVGNVDMSTLPKAVANRPPAPQSXXXXXXXXXXXXXXXXXXXXXX 120
           EVGHSLTASEQMEEYYVGNVDMSTLPKAVANRPPAPQSXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 61  EVGHSLTASEQMEEYYVGNVDMSTLPKAVANRPPAPQSXXXXXXXXXXXXXXXXXXXXXX 120

Query: 121 XXXXXXXXXPGSLKKVLQVLIPLLILGIAYYMQYYGKKQ 160
           XXXXXXXXXPGSLKKVLQVLIPLLILGIAYYMQYYGKKQ
Sbjct: 121 XXXXXXXXXPGSLKKVLQVLIPLLILGIAYYMQYYGKKQ 159

[Close]

BLAST of Carg25714 vs. NCBI nr
Match: XP_022979314.1 (cytochrome b5-like [Cucurbita maxima])

HSP 1 Score: 233.0 bits (593), Expect = 6.9e-58
Identity = 140/159 (88.05%), Postives = 145/159 (91.19%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFE 60
           MASDPKVFVFDEVAKHN+QQDCWVIISGKVYDVTSLLE+HPGGDEVLLLATERDATDDFE
Sbjct: 1   MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFE 60

Query: 61  EVGHSLTASEQMEEYYVGNVDMSTLPKAVANRPPAPQSXXXXXXXXXXXXXXXXXXXXXX 120
           EVGHSLTASEQMEEYYVGN+DM+TLPKAVANRPPAPQS          XXXXXXXXXXXX
Sbjct: 61  EVGHSLTASEQMEEYYVGNLDMATLPKAVANRPPAPQS--------GTXXXXXXXXXXXX 120

Query: 121 XXXXXXXXXPGSLKKVLQVLIPLLILGIAYYMQYYGKKQ 160
           XXXXXXX  PGSL KV QVLIPLLILGIA+YMQYYGKKQ
Sbjct: 121 XXXXXXXESPGSLIKVFQVLIPLLILGIAFYMQYYGKKQ 151

[Close]

BLAST of Carg25714 vs. NCBI nr
Match: XP_023527392.1 (cytochrome b5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 229.9 bits (585), Expect = 5.8e-57
Identity = 147/159 (92.45%), Postives = 151/159 (94.97%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFE 60
           MASDPKVFVFDEVAKHN+QQDCWVIISGKVYDVTS LEDHPGGDEVLLLATERDATDDFE
Sbjct: 1   MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDDFE 60

Query: 61  EVGHSLTASEQMEEYYVGNVDMSTLPKAVANRPPAPQSXXXXXXXXXXXXXXXXXXXXXX 120
           EVGHSLTASEQMEEYYVGN+DM+TLPKA ANRPPAPQ     XXXXXXXXXXXXXXXXXX
Sbjct: 61  EVGHSLTASEQMEEYYVGNLDMATLPKAGANRPPAPQ-----XXXXXXXXXXXXXXXXXX 120

Query: 121 XXXXXXXXXPGSLKKVLQVLIPLLILGIAYYMQYYGKKQ 160
           XXXXXXXXXPGSL KVLQVLIPLLILGIA+YMQYYGKKQ
Sbjct: 121 XXXXXXXXXPGSLIKVLQVLIPLLILGIAFYMQYYGKKQ 154

[Close]

BLAST of Carg25714 vs. NCBI nr
Match: XP_004133794.1 (PREDICTED: cytochrome b5 [Cucumis sativus] >XP_011650661.1 PREDICTED: cytochrome b5 [Cucumis sativus] >KGN56392.1 hypothetical protein Csa_3G119260 [Cucumis sativus])

HSP 1 Score: 197.6 bits (501), Expect = 3.2e-47
Identity = 107/159 (67.30%), Postives = 124/159 (77.99%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFE 60
           MASDPK+FVFDEVAKHN+Q DCW+IISGKVYDVT  LEDHPGGDEVLLLATE+DAT+DFE
Sbjct: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 61  EVGHSLTASEQMEEYYVGNVDMSTLPKAVANRPPAPQSXXXXXXXXXXXXXXXXXXXXXX 120
            V HSL A+E+ME+YY+GN+DMST+PK   +RPPA +S                    XX
Sbjct: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKS--------------ESAATEXX 120

Query: 121 XXXXXXXXXPGSLKKVLQVLIPLLILGIAYYMQYYGKKQ 160
           XXXXXX    GSL KVLQVLIPLLI+G+A+Y+QYYGKKQ
Sbjct: 121 XXXXXXAQSSGSLIKVLQVLIPLLIIGVAFYLQYYGKKQ 145

[Close]

BLAST of Carg25714 vs. NCBI nr
Match: XP_022147466.1 (cytochrome b5-like [Momordica charantia])

HSP 1 Score: 192.6 bits (488), Expect = 1.0e-45
Identity = 96/159 (60.38%), Postives = 114/159 (71.70%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFE 60
           M SDPKVF FDEVAKHN+Q+DCW+IISGKVYDVT  LE+HPGGDEVL+LA+E+DATDDFE
Sbjct: 1   MGSDPKVFAFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLILASEKDATDDFE 60

Query: 61  EVGHSLTASEQMEEYYVGNVDMSTLPKAVANRPPAPQSXXXXXXXXXXXXXXXXXXXXXX 120
            VGHSL+A +QME+YYVGNVDMST+PK +  RPPA QS                      
Sbjct: 61  NVGHSLSAIDQMEQYYVGNVDMSTVPKPIDYRPPASQS---------------------- 120

Query: 121 XXXXXXXXXPGSLKKVLQVLIPLLILGIAYYMQYYGKKQ 160
                     GS  K+LQ LIPLL++G+A+YMQ+YGKKQ
Sbjct: 121 ATTLASAQSSGSSLKLLQFLIPLLLIGVAFYMQFYGKKQ 137

[Close]

BLAST of Carg25714 vs. TAIR10
Match: AT5G53560.1 (cytochrome B5 isoform E)

HSP 1 Score: 135.6 bits (340), Expect = 2.7e-32
Identity = 71/161 (44.10%), Postives = 99/161 (61.49%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFE 60
           M+SD KV  F+EV+KHN  +DCW+IISGKVYDVT  ++DHPGGDEVLL +T +DAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  EVGHSLTASEQMEEYYVGNVDMSTLPKA---VANRPPAPQSXXXXXXXXXXXXXXXXXXX 120
           +VGHS TA + M++Y++G +D S++P     VA + PA                      
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPA---------------------- 120

Query: 121 XXXXXXXXXXXXPGSLKKVLQVLIPLLILGIAYYMQYYGKK 159
                       P  + K+LQ L+P+LILG+A  +++Y KK
Sbjct: 121 ------YNQDKTPEFIIKILQFLVPILILGLALVVRHYTKK 133

[Close]

BLAST of Carg25714 vs. TAIR10
Match: AT5G48810.1 (cytochrome B5 isoform D)

HSP 1 Score: 134.0 bits (336), Expect = 7.9e-32
Identity = 67/157 (42.68%), Postives = 92/157 (58.60%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFE 60
           M  D KVF   EV++H++ +DCW++I GKVYDVT  L+DHPGGDEV+L +T +DATDDFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  EVGHSLTASEQMEEYYVGNVDMSTLPKAVANRPPAPQSXXXXXXXXXXXXXXXXXXXXXX 120
           +VGHS TA   ++EYYVG++D +T+P      PP                          
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDF----------- 120

Query: 121 XXXXXXXXXPGSLKKVLQVLIPLLILGIAYYMQYYGK 158
                       + K+LQ L+PLLILG+A+ ++YY K
Sbjct: 121 ------------VIKLLQFLVPLLILGLAFGIRYYTK 134

[Close]

BLAST of Carg25714 vs. TAIR10
Match: AT2G32720.1 (cytochrome B5 isoform B)

HSP 1 Score: 131.7 bits (330), Expect = 3.9e-31
Identity = 67/157 (42.68%), Postives = 90/157 (57.32%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFE 60
           M  + K+F   EV++HN   DCW++I+GKVY+VT  LEDHPGGD+VLL +T +DATDDFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  EVGHSLTASEQMEEYYVGNVDMSTLPKAVANRPPAPQSXXXXXXXXXXXXXXXXXXXXXX 120
           +VGHS +A E ME+YYVG +D +T+PK V   PP                          
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEF------------- 120

Query: 121 XXXXXXXXXPGSLKKVLQVLIPLLILGIAYYMQYYGK 158
                       + K+LQ L+PL ILG+A  ++ Y K
Sbjct: 121 ------------IIKLLQFLVPLAILGLAVGIRIYTK 132

[Close]

BLAST of Carg25714 vs. TAIR10
Match: AT1G26340.1 (cytochrome B5 isoform A)

HSP 1 Score: 110.9 bits (276), Expect = 7.2e-25
Identity = 46/82 (56.10%), Postives = 63/82 (76.83%), Query Frame = 0


Query: 6  KVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFEEVGHS 65
          K++  +E A HN Q DCWV+I GKVYDV+S +++HPGGD+VLL    +DATDDFE+ GHS
Sbjct: 6  KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 66 LTASEQMEEYYVGNVDMSTLPK 88
            A E ME+Y++G +D S+LP+
Sbjct: 66 KDARELMEKYFIGELDESSLPE 87

[Close]

BLAST of Carg25714 vs. TAIR10
Match: AT2G46650.1 (cytochrome B5 isoform C)

HSP 1 Score: 110.2 bits (274), Expect = 1.2e-24
Identity = 58/149 (38.93%), Postives = 83/149 (55.70%), Query Frame = 0


Query: 10  FDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFEEVGHSLTAS 69
           F +VAKH  + DCW++I GKVYD+++ +++HPGGD VLL  T +DA+ DFE+V HS  A 
Sbjct: 7   FHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSKDAK 66

Query: 70  EQMEEYYVGNVDMSTLPKAVANRPPAPQSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 129
           E M++Y +G+VD ST+P      PP  +                                
Sbjct: 67  ELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEE--------------------- 126

Query: 130 PGSLKKVLQVLIPLLILGIAYYMQYYGKK 159
             S KK+L  LIPLLILG+A+ +++Y  K
Sbjct: 127 --SGKKLLIYLIPLLILGVAFALRFYNNK 132

[Close]

BLAST of Carg25714 vs. Swiss-Prot
Match: sp|P40934|CYB5_BRAOB (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 2.9e-31
Identity = 70/159 (44.03%), Postives = 96/159 (60.38%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFE 60
           MAS+ KV  F+EV++HN  +DCW+IISGKVYDVT  ++DHPGGDEVLL +T +DAT+DFE
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  EVGHSLTASEQMEEYYVGNVDMSTLPKAVANRPPAPQSXXXXXXXXXXXXXXXXXXXXXX 120
           +VGHS TA + ME+YY+G +D ST+P       P   +                      
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVPATRTYVAPVQPA---------------------- 120

Query: 121 XXXXXXXXXPGSLKKVLQVLIPLLILGIAYYMQYYGKKQ 160
                    P  + K+LQ L+P+LILG+A  ++ Y KK+
Sbjct: 121 ---YNQDKTPEFMIKILQFLVPILILGLALVVRQYTKKE 134

[Close]

BLAST of Carg25714 vs. Swiss-Prot
Match: sp|P49098|CYB5_TOBAC (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 3.8e-31
Identity = 68/158 (43.04%), Postives = 94/158 (59.49%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFE 60
           M  + KVF   EV++HNN +DCW++ISGKVYDVT  L+DHPGGDEVLL AT +DATDDFE
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  EVGHSLTASEQMEEYYVGNVDMSTLPKAVANRPPAPQSXXXXXXXXXXXXXXXXXXXXXX 120
           +VGHS +A   ++EYYVG++D +T+P      PP                          
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEF------------- 120

Query: 121 XXXXXXXXXPGSLKKVLQVLIPLLILGIAYYMQYYGKK 159
                       + K+LQ L+PL+ILG+A+ +++Y K+
Sbjct: 121 ------------VVKLLQFLVPLIILGVAFGIRFYTKQ 133

[Close]

BLAST of Carg25714 vs. Swiss-Prot
Match: sp|Q42342|CYB5E_ARATH (Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 135.6 bits (340), Expect = 4.9e-31
Identity = 71/161 (44.10%), Postives = 99/161 (61.49%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFE 60
           M+SD KV  F+EV+KHN  +DCW+IISGKVYDVT  ++DHPGGDEVLL +T +DAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  EVGHSLTASEQMEEYYVGNVDMSTLPKA---VANRPPAPQSXXXXXXXXXXXXXXXXXXX 120
           +VGHS TA + M++Y++G +D S++P     VA + PA                      
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPA---------------------- 120

Query: 121 XXXXXXXXXXXXPGSLKKVLQVLIPLLILGIAYYMQYYGKK 159
                       P  + K+LQ L+P+LILG+A  +++Y KK
Sbjct: 121 ------YNQDKTPEFIIKILQFLVPILILGLALVVRHYTKK 133

[Close]

BLAST of Carg25714 vs. Swiss-Prot
Match: sp|P49099|CYB5S_TOBAC (Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.1e-30
Identity = 69/158 (43.67%), Postives = 91/158 (57.59%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFE 60
           M    KVF   EV+ HNN +DCW+IISGKVY+VT  LEDHPGG EVLL AT +DATDDFE
Sbjct: 1   MGGQSKVFTLAEVSNHNNAKDCWLIISGKVYNVTKFLEDHPGGGEVLLSATGKDATDDFE 60

Query: 61  EVGHSLTASEQMEEYYVGNVDMSTLPKAVANRPPAPQSXXXXXXXXXXXXXXXXXXXXXX 120
           ++GHS +A   ++EYYVG++D ST+P  V   PP                          
Sbjct: 61  DIGHSSSARAMLDEYYVGDIDSSTIPTKVKYTPPKQPHYNQDKTTEF------------- 120

Query: 121 XXXXXXXXXPGSLKKVLQVLIPLLILGIAYYMQYYGKK 159
                       + K+LQ L+PL+ILG+A+ + +Y K+
Sbjct: 121 ------------IVKLLQFLVPLIILGVAFGVHFYTKQ 133

[Close]

BLAST of Carg25714 vs. Swiss-Prot
Match: sp|Q9ZWT2|CYB5D_ARATH (Cytochrome B5 isoform D OS=Arabidopsis thaliana OX=3702 GN=CYTB5-D PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.4e-30
Identity = 67/157 (42.68%), Postives = 92/157 (58.60%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFE 60
           M  D KVF   EV++H++ +DCW++I GKVYDVT  L+DHPGGDEV+L +T +DATDDFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  EVGHSLTASEQMEEYYVGNVDMSTLPKAVANRPPAPQSXXXXXXXXXXXXXXXXXXXXXX 120
           +VGHS TA   ++EYYVG++D +T+P      PP                          
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDF----------- 120

Query: 121 XXXXXXXXXPGSLKKVLQVLIPLLILGIAYYMQYYGK 158
                       + K+LQ L+PLLILG+A+ ++YY K
Sbjct: 121 ------------VIKLLQFLVPLLILGLAFGIRYYTK 134

[Close]

BLAST of Carg25714 vs. TrEMBL
Match: tr|A0A0A0L720|A0A0A0L720_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G119260 PE=3 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 2.1e-47
Identity = 107/159 (67.30%), Postives = 124/159 (77.99%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFE 60
           MASDPK+FVFDEVAKHN+Q DCW+IISGKVYDVT  LEDHPGGDEVLLLATE+DAT+DFE
Sbjct: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 61  EVGHSLTASEQMEEYYVGNVDMSTLPKAVANRPPAPQSXXXXXXXXXXXXXXXXXXXXXX 120
            V HSL A+E+ME+YY+GN+DMST+PK   +RPPA +S                    XX
Sbjct: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKS--------------ESAATEXX 120

Query: 121 XXXXXXXXXPGSLKKVLQVLIPLLILGIAYYMQYYGKKQ 160
           XXXXXX    GSL KVLQVLIPLLI+G+A+Y+QYYGKKQ
Sbjct: 121 XXXXXXAQSSGSLIKVLQVLIPLLIIGVAFYLQYYGKKQ 145

[Close]

BLAST of Carg25714 vs. TrEMBL
Match: tr|A0A1S3AV33|A0A1S3AV33_CUCME (cytochrome b5-like OS=Cucumis melo OX=3656 GN=LOC103483149 PE=3 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.5e-37
Identity = 77/98 (78.57%), Postives = 90/98 (91.84%), Query Frame = 0


Query: 1  MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFE 60
          MASDPK+FVFDEVAKHN+Q DCW+IISGKVYDVT  LEDHPGGDEVLLLATE+DAT+DFE
Sbjct: 1  MASDPKLFVFDEVAKHNHQGDCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 61 EVGHSLTASEQMEEYYVGNVDMSTLPKAVANRPPAPQS 99
           VGHSL+A+E+ME+YY+GN+DMST+PK V  RPPA +S
Sbjct: 61 TVGHSLSATEEMEKYYIGNIDMSTVPK-VDYRPPASKS 97

[Close]

BLAST of Carg25714 vs. TrEMBL
Match: tr|M5X125|M5X125_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_2G194100 PE=3 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 7.6e-37
Identity = 84/159 (52.83%), Postives = 106/159 (66.67%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFE 60
           MASD +V+ FDEVAKHN+++DCW+IISGKVYDVT  LEDHPGGDEVLLLA E+DATDDF 
Sbjct: 1   MASDSQVYHFDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKDATDDFN 60

Query: 61  EVGHSLTASEQMEEYYVGNVDMSTLPKAVANRPPAPQSXXXXXXXXXXXXXXXXXXXXXX 120
           +VGHS +A EQME++YVG VD ST+P+  + + PA  +                      
Sbjct: 61  DVGHSDSAKEQMEKFYVGKVDTSTIPEQPSYKLPAQTT---------------------- 120

Query: 121 XXXXXXXXXPGSLKKVLQVLIPLLILGIAYYMQYYGKKQ 160
                     G + K+LQ L+PLLILG A+ +QYYGKK+
Sbjct: 121 ---PPSGQSSGFVVKLLQFLLPLLILGAAFALQYYGKKK 134

[Close]

BLAST of Carg25714 vs. TrEMBL
Match: tr|A0A2P5FIE4|A0A2P5FIE4_9ROSA (Nitrate reductase NADH dependent OS=Trema orientalis OX=63057 GN=TorRG33x02_067620 PE=3 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 2.9e-36
Identity = 83/159 (52.20%), Postives = 103/159 (64.78%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFE 60
           MAS  KVF FDEVA+HN+++DCW+IISGK YDVT  LE+HPGGDEVLLLA E+DATDDFE
Sbjct: 1   MASGSKVFTFDEVAQHNHKKDCWIIISGKAYDVTEFLEEHPGGDEVLLLAAEKDATDDFE 60

Query: 61  EVGHSLTASEQMEEYYVGNVDMSTLPKAVANRPPAPQSXXXXXXXXXXXXXXXXXXXXXX 120
           +VGHS  A E +++Y VG VD+STLP+    +PP   S                      
Sbjct: 61  DVGHSDDAKELLKKYCVGEVDLSTLPEKPNYKPPTQSS---------------------- 120

Query: 121 XXXXXXXXXPGSLKKVLQVLIPLLILGIAYYMQYYGKKQ 160
                     GS  K+LQ L+PLLILGIA+ +Q+YGKK+
Sbjct: 121 ----QSDQSSGSAVKILQFLLPLLILGIAFALQFYGKKK 133

[Close]

BLAST of Carg25714 vs. TrEMBL
Match: tr|A0A2P5DQA7|A0A2P5DQA7_PARAD (Nitrate reductase NADH dependent OS=Parasponia andersonii OX=3476 GN=PanWU01x14_040920 PE=3 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 3.8e-36
Identity = 83/159 (52.20%), Postives = 103/159 (64.78%), Query Frame = 0


Query: 1   MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFE 60
           MAS  KVF FDEVA+HN+++DCW+IISGK YDVT  LE+HPGGDEVLLLA E+DATDDFE
Sbjct: 1   MASGSKVFTFDEVAQHNHKKDCWIIISGKAYDVTEFLEEHPGGDEVLLLAAEKDATDDFE 60

Query: 61  EVGHSLTASEQMEEYYVGNVDMSTLPKAVANRPPAPQSXXXXXXXXXXXXXXXXXXXXXX 120
           +VGHS  A E +++Y VG VD+STLP+    +PP   S                      
Sbjct: 61  DVGHSDDAKELLKKYCVGEVDLSTLPEKPNYKPPTHSS---------------------- 120

Query: 121 XXXXXXXXXPGSLKKVLQVLIPLLILGIAYYMQYYGKKQ 160
                     GS  K+LQ L+PLLILGIA+ +Q+YGKK+
Sbjct: 121 ---SQSDQSSGSAVKILQFLLPLLILGIAFALQFYGKKK 134

The following BLAST results are available for this feature:

BLAST of Carg25714 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022924404.1	9.3e-63	100.00	cytochrome b5-like [Cucurbita moschata]	[more]
XP_022979314.1	6.9e-58	88.05	cytochrome b5-like [Cucurbita maxima]	[more]
XP_023527392.1	5.8e-57	92.45	cytochrome b5-like [Cucurbita pepo subsp. pepo]	[more]
XP_004133794.1	3.2e-47	67.30	PREDICTED: cytochrome b5 [Cucumis sativus] >XP_011650661.1 PREDICTED: cytochrome...	[more]
XP_022147466.1	1.0e-45	60.38	cytochrome b5-like [Momordica charantia]	[more]

BLAST of Carg25714 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G53560.1	2.7e-32	44.10	cytochrome B5 isoform E	[more]
AT5G48810.1	7.9e-32	42.68	cytochrome B5 isoform D	[more]
AT2G32720.1	3.9e-31	42.68	cytochrome B5 isoform B	[more]
AT1G26340.1	7.2e-25	56.10	cytochrome B5 isoform A	[more]
AT2G46650.1	1.2e-24	38.93	cytochrome B5 isoform C	[more]

BLAST of Carg25714 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|P40934\|CYB5_BRAOB	2.9e-31	44.03	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]
sp\|P49098\|CYB5_TOBAC	3.8e-31	43.04	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
sp\|Q42342\|CYB5E_ARATH	4.9e-31	44.10	Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2	[more]
sp\|P49099\|CYB5S_TOBAC	1.1e-30	43.67	Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
sp\|Q9ZWT2\|CYB5D_ARATH	1.4e-30	42.68	Cytochrome B5 isoform D OS=Arabidopsis thaliana OX=3702 GN=CYTB5-D PE=1 SV=1	[more]

BLAST of Carg25714 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0L720\|A0A0A0L720_CUCSA	2.1e-47	67.30	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G119260 PE=3 SV=1	[more]
tr\|A0A1S3AV33\|A0A1S3AV33_CUCME	1.5e-37	78.57	cytochrome b5-like OS=Cucumis melo OX=3656 GN=LOC103483149 PE=3 SV=1	[more]
tr\|M5X125\|M5X125_PRUPE	7.6e-37	52.83	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_2G194100 PE=3 SV=1	[more]
tr\|A0A2P5FIE4\|A0A2P5FIE4_9ROSA	2.9e-36	52.20	Nitrate reductase NADH dependent OS=Trema orientalis OX=63057 GN=TorRG33x02_0676...	[more]
tr\|A0A2P5DQA7\|A0A2P5DQA7_PARAD	3.8e-36	52.20	Nitrate reductase NADH dependent OS=Parasponia andersonii OX=3476 GN=PanWU01x14_...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR036400	Cyt_B5-like_heme/steroid_sf
IPR001199	Cyt_B5-like_heme/steroid-bd

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25714-RA	Carg25714-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 55..62 score: 44.13 coord: 40..54 score: 46.57 coord: 68..80 score: 31.0 coord: 30..40 score: 59.78
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 e-value: 7.1E-27 score: 105.3
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 11..80 e-value: 3.2E-22 score: 78.4
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 26.852
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	G3DSA:3.10.120.10		coord: 1..87 e-value: 1.3E-30 score: 107.0
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	SSF55856	Cytochrome b5-like heme/steroid binding domain	coord: 4..88
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 116..131
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 87..131
None	No IPR available	PANTHER	PTHR19359:SF43	CYTOCHROME B5 ISOFORM C	coord: 113..158
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..105
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 113..158
None	No IPR available	PANTHER	PTHR19359:SF43	CYTOCHROME B5 ISOFORM C	coord: 1..105

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg25714	Cla021123	Watermelon (97103) v1	carwmB0122
Carg25714	Csa3G119260	Cucumber (Chinese Long) v2	carcuB0132
Carg25714	MELO3C005994	Melon (DHL92) v3.5.1	carmeB0128
Carg25714	ClCG05G000550	Watermelon (Charleston Gray)	carwcgB0122
Carg25714	CSPI03G07620	Wild cucumber (PI 183967)	carcpiB0137
Carg25714	Cucsa.259540	Cucumber (Gy14) v1	carcgyB0148
Carg25714	CmaCh06G009870	Cucurbita maxima (Rimu)	carcmaB0163
Carg25714	CmaCh14G021300	Cucurbita maxima (Rimu)	carcmaB0162
Carg25714	CmoCh06G010160	Cucurbita moschata (Rifu)	carcmoB0171
Carg25714	CmoCh14G021930	Cucurbita moschata (Rifu)	carcmoB0170
Carg25714	Lsi05G021600	Bottle gourd (USVL1VR-Ls)	carlsiB110
Carg25714	Cp4.1LG03g16000	Cucurbita pepo (Zucchini)	carcpeB0179
Carg25714	Cp4.1LG08g00630	Cucurbita pepo (Zucchini)	carcpeB0180
Carg25714	CsGy3G007780	Cucumber (Gy14) v2	carcgybB0131
Carg25714	MELO3C005994.2	Melon (DHL92) v3.6.1	carmedB0132
Carg25714	CsaV3_3G007770	Cucumber (Chinese Long) v3	carcucB0141
Carg25714	Bhi12G000587	Wax gourd	carwgoB0152
Carg25714	Bhi01G000393	Wax gourd	carwgoB0153

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg25714	Carg20915	Silver-seed gourd	carcarB146

The following block(s) are covering this gene:


Gene	Organism	Block
Carg25714	Cucurbita maxima (Rimu)	carcmaB0164
Carg25714	Watermelon (97103) v2	carwmbB0130