CmaCh14G021300 (gene) Cucurbita maxima (Rimu)

Overview
NameCmaCh14G021300
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionCytochrome b5
LocationCma_Chr14 : 14625342 .. 14627189 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAACAAGGACTGAATTGAAATTGTTGCAAGAAAACAAATCCATTCGCGGGGTGTCATTTAGCCAGCGAAACCGGCCAGATAAAAGTCGCAAGAAATGGCTGGTAGTGGTAGTCAATGGAGAAGAAATCGTGCTGATTTGGGCTGTTTCTCCAATTTCCTTCAACGTTCATTCTGTAAGTAACCCCACATTCTTCCTCGGTTCTTCCGTGTTTGATTCCTCTGATTCTCTAATTTAATCGACTGCTTCATTGTGGATTGAGCTTGATTTTTATTTTCAGTTGATTGTTTAGGAAATGGCTTCGGATCCTAAGGTTTTCGTCTTCGACGAAGTGGCTAAACACAATCACCAGCAGGATTGTTGGGTTATAATCTCTGGAAAGGTCTGATTTAACAATCTCTCTTCTGATCTTCATTTTGTGAAATTGTTTGTTCAGAACTCAAATCGTATTTGCCAATGGAACAGTACACTTAATTGCGATCCGATAGGTGGATGGGAGATTAGGCCTATTTGTTTCTTGATCTTTCTCCTAACAATCTTTGAACTCATTACCCGAGAAACGACAGGAACTTGTTCTTCATCTTTTCTTCTGATGTGAAATCAGTTGTTGAGGATTAAAACATAAATCCTATGCATGTGTGAGACGTATTTCGTGGAATTCATTTACCAAGCACTTGTTCAAGAGGAAAAACTCTGGTATTGTAGTTTGCAATGAATCTAAGCTCCAACTTTCATCAAAGAAGAATTTTAGGCCTCACAGTTCTTTTAAGTTTAACAACATAGGAAGGAGATTTGATGTCCTCACTTAATCACATTTTCTCTCCCTTAAAACACCGATTTGGCCTCAAACATTCCATTTGCTCGAAATTTGCAATTTAAACAACCATGCTCGTGAAATTCACTAATTCAACCACAAAACCGTGCAAAATCTTAGTCCTGGTAACAACAGATTATGTGTACTATCAGTTATCTCAGTATTCTTCTCCTAGATATACCTAAAATAAGACGCTTTCAATTTGGGTGATAAGATTTGTATTTTTTCTATCCTCTTCCTTTCTATCGTTGTTGTGTTTCGTTGCCTTTCCCCAAAGTAGTTTTTGTGATCCATGTTAGGTGTATGATGTCACCTCCCTTCTAGAAGAGCATCCTGGAGGTGATGAAGTCTTGCTGTTAGCTACTGGTAAGTCGAACTTGATAACTTGAATGTGTAACTGCGTCAGAGCAAATGGAAGAATATGATCACACCACGTTATGAATGATTGGAGAAACAATCTTGAATGTGTGCAGAAAGAGATGCCACTGATGATTTTGAAGAAGTGGGTCACAGTTTAACTGCGTCAGAGCAAATGGAAGAATATTATGTTGGTAATCTGGACATGGCTACCCTTCCAAAGGCAGTTGCCAATCGACCACCAGCACCCCAATCGGGGACAGCGACAACAGCAGCAGCAAAACCAACAGCAGCAGCACCAACAACAACGTCATCAGCTGAATCTCCAGGATCTTTGATAAAAGTATTTCAAGTTCTGATTCCCCTGCTGATTTTGGGTATTGCATTTTATATGCAATACTATGGCAAGAAACAGTAAGCGAAATTTGTCCAACCATAGATGCCTTGAGTTTTGCAATAAGCCAGAGCTGTGTAGTAGAAATTCTATCATGTTCTGTCGAAGACATTCTTTGTTATCTATCTAGGTTTCTCGGCCACAGCCTTCTTGGTAATATCTGAGAAGGGAATTTTCATTTGCCTCTTCTTCATTTCTTATCCTGTTTTCTGTCTTTTGTTCTACAATATTCAACTTTGTAAATAAATAATCATGATTATTTCTAAAATACTTATTTCTCG

mRNA sequence

ATGAAAACAAGGACTGAATTGAAATTGTTGCAAGAAAACAAATCCATTCGCGGGGTGTCATTTAGCCAGCGAAACCGGCCAGATAAAAGTCGCAAGAAATGGCTGGTAGTGGTAGTCAATGGAGAAGAAATCGTGCTGATTTGGGCTGTTTCTCCAATTTCCTTCAACGTTCATTCTGAAATGGCTTCGGATCCTAAGGTTTTCGTCTTCGACGAAGTGGCTAAACACAATCACCAGCAGGATTGTTGGGTTATAATCTCTGGAAAGGTGTATGATGTCACCTCCCTTCTAGAAGAGCATCCTGGAGGTGATGAAGTCTTGCTGTTAGCTACTGAAAGAGATGCCACTGATGATTTTGAAGAAGTGGGTCACAGTTTAACTGCGTCAGAGCAAATGGAAGAATATTATGTTGGTAATCTGGACATGGCTACCCTTCCAAAGGCAGTTGCCAATCGACCACCAGCACCCCAATCGGGGACAGCGACAACAGCAGCAGCAAAACCAACAGCAGCAGCACCAACAACAACGTCATCAGCTGAATCTCCAGGATCTTTGATAAAAGTATTTCAAGTTCTGATTCCCCTGCTGATTTTGGGTATTGCATTTTATATGCAATACTATGGCAAGAAACAGTAAGCGAAATTTGTCCAACCATAGATGCCTTGAGTTTTGCAATAAGCCAGAGCTGTGTAGTAGAAATTCTATCATGTTCTGTCGAAGACATTCTTTGTTATCTATCTAGGTTTCTCGGCCACAGCCTTCTTGGTAATATCTGAGAAGGGAATTTTCATTTGCCTCTTCTTCATTTCTTATCCTGTTTTCTGTCTTTTGTTCTACAATATTCAACTTTGTAAATAAATAATCATGATTATTTCTAAAATACTTATTTCTCG

Coding sequence (CDS)

ATGAAAACAAGGACTGAATTGAAATTGTTGCAAGAAAACAAATCCATTCGCGGGGTGTCATTTAGCCAGCGAAACCGGCCAGATAAAAGTCGCAAGAAATGGCTGGTAGTGGTAGTCAATGGAGAAGAAATCGTGCTGATTTGGGCTGTTTCTCCAATTTCCTTCAACGTTCATTCTGAAATGGCTTCGGATCCTAAGGTTTTCGTCTTCGACGAAGTGGCTAAACACAATCACCAGCAGGATTGTTGGGTTATAATCTCTGGAAAGGTGTATGATGTCACCTCCCTTCTAGAAGAGCATCCTGGAGGTGATGAAGTCTTGCTGTTAGCTACTGAAAGAGATGCCACTGATGATTTTGAAGAAGTGGGTCACAGTTTAACTGCGTCAGAGCAAATGGAAGAATATTATGTTGGTAATCTGGACATGGCTACCCTTCCAAAGGCAGTTGCCAATCGACCACCAGCACCCCAATCGGGGACAGCGACAACAGCAGCAGCAAAACCAACAGCAGCAGCACCAACAACAACGTCATCAGCTGAATCTCCAGGATCTTTGATAAAAGTATTTCAAGTTCTGATTCCCCTGCTGATTTTGGGTATTGCATTTTATATGCAATACTATGGCAAGAAACAGTAA

Protein sequence

MKTRTELKLLQENKSIRGVSFSQRNRPDKSRKKWLVVVVNGEEIVLIWAVSPISFNVHSEMASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFEEVGHSLTASEQMEEYYVGNLDMATLPKAVANRPPAPQSGTATTAAAKPTAAAPTTTSSAESPGSLIKVFQVLIPLLILGIAFYMQYYGKKQ
Homology
[Close]
BLAST of CmaCh14G021300 vs. Swiss-Prot
Match: CYB5D_ARATH (Cytochrome B5 isoform D OS=Arabidopsis thaliana GN=CYTB5-D PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.0e-32
Identity = 73/149 (48.99%), Postives = 99/149 (66.44%), Query Frame = 1 

Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFE 120
           M  D KVF   EV++H+  +DCW++I GKVYDVT  L++HPGGDEV+L +T +DATDDFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAVANRPPAPQSGTATTAAAKPTAAAPTTTSSAE 180
           +VGHS TA   ++EYYVG++D AT+P      PP     T+T A A          +  +
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPP-----TSTKAVA----------TQDK 120

Query: 181 SPGSLIKVFQVLIPLLILGIAFYMQYYGK 210
           S   +IK+ Q L+PLLILG+AF ++YY K
Sbjct: 121 SSDFVIKLLQFLVPLLILGLAFGIRYYTK 134

[Close]
BLAST of CmaCh14G021300 vs. Swiss-Prot
Match: CYB5_BRAOB (Cytochrome b5 OS=Brassica oleracea var. botrytis GN=CYB5 PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 3.8e-31
Identity = 71/151 (47.02%), Postives = 101/151 (66.89%), Query Frame = 1 

Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFE 120
           MAS+ KV  F+EV++HN  +DCW+IISGKVYDVT  +++HPGGDEVLL +T +DAT+DFE
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAVANRPPAPQSGTATTAAAKPTAAAPTTTSSAE 180
           +VGHS TA + ME+YY+G +D +T+P              AT     P   A    +  +
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVP--------------ATRTYVAPVQPA---YNQDK 120

Query: 181 SPGSLIKVFQVLIPLLILGIAFYMQYYGKKQ 212
           +P  +IK+ Q L+P+LILG+A  ++ Y KK+
Sbjct: 121 TPEFMIKILQFLVPILILGLALVVRQYTKKE 134

[Close]
BLAST of CmaCh14G021300 vs. Swiss-Prot
Match: CYB5_TOBAC (Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.4e-30
Identity = 69/150 (46.00%), Postives = 98/150 (65.33%), Query Frame = 1 

Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFE 120
           M  + KVF   EV++HN+ +DCW++ISGKVYDVT  L++HPGGDEVLL AT +DATDDFE
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAVANRPPAPQSGTATTAAAKPTAAAPTTTSSAE 180
           +VGHS +A   ++EYYVG++D AT+P      PP            +P      T+    
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPP-----------NQPHYNQDKTSE--- 120

Query: 181 SPGSLIKVFQVLIPLLILGIAFYMQYYGKK 211
               ++K+ Q L+PL+ILG+AF +++Y K+
Sbjct: 121 ---FVVKLLQFLVPLIILGVAFGIRFYTKQ 133

[Close]
BLAST of CmaCh14G021300 vs. Swiss-Prot
Match: CYB5E_ARATH (Cytochrome b5 isoform E OS=Arabidopsis thaliana GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 134.0 bits (336), Expect = 1.9e-30
Identity = 69/150 (46.00%), Postives = 101/150 (67.33%), Query Frame = 1 

Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFE 120
           M+SD KV  F+EV+KHN  +DCW+IISGKVYDVT  +++HPGGDEVLL +T +DAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAVANRPPAPQSGTATTAAAKPTAAAPTTTSSAE 180
           +VGHS TA + M++Y++G +D +++P              AT     P   A    +  +
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVP--------------ATRTYVAPQQPA---YNQDK 120

Query: 181 SPGSLIKVFQVLIPLLILGIAFYMQYYGKK 211
           +P  +IK+ Q L+P+LILG+A  +++Y KK
Sbjct: 121 TPEFIIKILQFLVPILILGLALVVRHYTKK 133

[Close]
BLAST of CmaCh14G021300 vs. Swiss-Prot
Match: CYB5S_TOBAC (Cytochrome b5, seed isoform OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 7.1e-30
Identity = 69/150 (46.00%), Postives = 95/150 (63.33%), Query Frame = 1 

Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFE 120
           M    KVF   EV+ HN+ +DCW+IISGKVY+VT  LE+HPGG EVLL AT +DATDDFE
Sbjct: 1   MGGQSKVFTLAEVSNHNNAKDCWLIISGKVYNVTKFLEDHPGGGEVLLSATGKDATDDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAVANRPPAPQSGTATTAAAKPTAAAPTTTSSAE 180
           ++GHS +A   ++EYYVG++D +T+P  V   PP            +P      TT    
Sbjct: 61  DIGHSSSARAMLDEYYVGDIDSSTIPTKVKYTPP-----------KQPHYNQDKTTE--- 120

Query: 181 SPGSLIKVFQVLIPLLILGIAFYMQYYGKK 211
               ++K+ Q L+PL+ILG+AF + +Y K+
Sbjct: 121 ---FIVKLLQFLVPLIILGVAFGVHFYTKQ 133

[Close]
BLAST of CmaCh14G021300 vs. TrEMBL
Match: A0A0A0L720_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G119260 PE=3 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 5.4e-53
Identity = 108/151 (71.52%), Postives = 127/151 (84.11%), Query Frame = 1 

Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFE 120
           MASDPK+FVFDEVAKHNHQ DCW+IISGKVYDVT  LE+HPGGDEVLLLATE+DAT+DFE
Sbjct: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAVANRPPAPQSGTATTAAAKPTAAAPTTTSSAE 180
            V HSL A+E+ME+YY+GN+DM+T+PK   +RPPA +S +A       T AA   TSSA+
Sbjct: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAA------TEAATEKTSSAQ 120

Query: 181 SPGSLIKVFQVLIPLLILGIAFYMQYYGKKQ 212
           S GSLIKV QVLIPLLI+G+AFY+QYYGKKQ
Sbjct: 121 SSGSLIKVLQVLIPLLIIGVAFYLQYYGKKQ 145

[Close]
BLAST of CmaCh14G021300 vs. TrEMBL
Match: M5X125_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013234mg PE=3 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 9.0e-40
Identity = 86/151 (56.95%), Postives = 110/151 (72.85%), Query Frame = 1 

Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFE 120
           MASD +V+ FDEVAKHNH++DCW+IISGKVYDVT  LE+HPGGDEVLLLA E+DATDDF 
Sbjct: 1   MASDSQVYHFDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKDATDDFN 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAVANRPPAPQSGTATTAAAKPTAAAPTTTSSAE 180
           +VGHS +A EQME++YVG +D +T+P+  + + P                 A TT  S +
Sbjct: 61  DVGHSDSAKEQMEKFYVGKVDTSTIPEQPSYKLP-----------------AQTTPPSGQ 120

Query: 181 SPGSLIKVFQVLIPLLILGIAFYMQYYGKKQ 212
           S G ++K+ Q L+PLLILG AF +QYYGKK+
Sbjct: 121 SSGFVVKLLQFLLPLLILGAAFALQYYGKKK 134

[Close]
BLAST of CmaCh14G021300 vs. TrEMBL
Match: W9RDL9_9ROSA (Cytochrome b5, seed isoform OS=Morus notabilis GN=L484_023457 PE=3 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 1.2e-39
Identity = 89/151 (58.94%), Postives = 110/151 (72.85%), Query Frame = 1 

Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFE 120
           MA D KVF FDEVAKHNH++DCW+IISGKVY+VT  LEEHPGGDEVLLLA E+DATDDFE
Sbjct: 1   MARDSKVFAFDEVAKHNHKKDCWIIISGKVYNVTEFLEEHPGGDEVLLLAAEKDATDDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAVANRPPAPQSGTATTAAAKPTAAAPTTTSSAE 180
           +VGHS TA E ME+YYVG +D++TLP+  + +PP PQ               P + S+  
Sbjct: 61  DVGHSDTAREMMEKYYVGKVDVSTLPEKPSYKPP-PQ---------------PPSQSNQS 120

Query: 181 SPGSLIKVFQVLIPLLILGIAFYMQYYGKKQ 212
           S GS +K+ Q L+PLLIL IAF + +YGK +
Sbjct: 121 SGGS-VKILQFLLPLLILAIAFALHFYGKSK 134

[Close]
BLAST of CmaCh14G021300 vs. TrEMBL
Match: A0A151SWJ2_CAJCA (Cytochrome b5 OS=Cajanus cajan GN=KK1_014582 PE=3 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.0e-35
Identity = 79/150 (52.67%), Postives = 102/150 (68.00%), Query Frame = 1 

Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFE 120
           M S  K F F+EVAKHNH++DCW+I++GKVYDVT  LE+HPGGDEVL+ ATE+DAT DFE
Sbjct: 1   MGSKTKTFTFEEVAKHNHRKDCWIIVNGKVYDVTPFLEDHPGGDEVLVTATEKDATSDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAVANRPPAPQSGTATTAAAKPTAAAPTTTSSAE 180
           ++GHS +A E M+ Y VG +D  TLP    N P             +P   AP   +S +
Sbjct: 61  DIGHSESAIEMMQTYLVGEVDTNTLPTQATNTP-------------RPPTQAP--ANSNQ 120

Query: 181 SPGSLIKVFQVLIPLLILGIAFYMQYYGKK 211
           S G ++K+ Q L+PLLILG AF +QYYGK+
Sbjct: 121 SAGFVLKMLQYLVPLLILGFAFALQYYGKR 135

[Close]
BLAST of CmaCh14G021300 vs. TrEMBL
Match: I1NCP5_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_19G258500 PE=3 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 2.3e-35
Identity = 79/151 (52.32%), Postives = 108/151 (71.52%), Query Frame = 1 

Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFE 120
           MAS+PK   F+EVAKHN+++DCW+II+GKVYD+T  L+EHPGGDEVLL +T +DAT DFE
Sbjct: 1   MASNPKTLTFEEVAKHNNKKDCWIIINGKVYDITPFLDEHPGGDEVLLTSTGKDATIDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAVANRPPAPQSGTATTAAAKPTAAAPTTTSSAE 180
           +VGHS +A E ME+Y++G +D +TLP  V++  P P   T T  A              +
Sbjct: 61  DVGHSDSAIEMMEKYFIGKVDTSTLPPKVSHSLPQP---TQTHGAGN------------Q 120

Query: 181 SPGSLIKVFQVLIPLLILGIAFYMQYYGKKQ 212
           S G ++K+ Q L+PLLILG+AF +QYYG+K+
Sbjct: 121 SSGFVVKILQFLLPLLILGLAFALQYYGQKK 136

[Close]
BLAST of CmaCh14G021300 vs. TAIR10
Match: AT5G48810.1 (AT5G48810.1 cytochrome B5 isoform D)

HSP 1 Score: 140.6 bits (353), Expect = 1.1e-33
Identity = 73/149 (48.99%), Postives = 99/149 (66.44%), Query Frame = 1 

Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFE 120
           M  D KVF   EV++H+  +DCW++I GKVYDVT  L++HPGGDEV+L +T +DATDDFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAVANRPPAPQSGTATTAAAKPTAAAPTTTSSAE 180
           +VGHS TA   ++EYYVG++D AT+P      PP     T+T A A          +  +
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPP-----TSTKAVA----------TQDK 120

Query: 181 SPGSLIKVFQVLIPLLILGIAFYMQYYGK 210
           S   +IK+ Q L+PLLILG+AF ++YY K
Sbjct: 121 SSDFVIKLLQFLVPLLILGLAFGIRYYTK 134

[Close]
BLAST of CmaCh14G021300 vs. TAIR10
Match: AT5G53560.1 (AT5G53560.1 cytochrome B5 isoform E)

HSP 1 Score: 134.0 bits (336), Expect = 1.1e-31
Identity = 69/150 (46.00%), Postives = 101/150 (67.33%), Query Frame = 1 

Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFE 120
           M+SD KV  F+EV+KHN  +DCW+IISGKVYDVT  +++HPGGDEVLL +T +DAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAVANRPPAPQSGTATTAAAKPTAAAPTTTSSAE 180
           +VGHS TA + M++Y++G +D +++P              AT     P   A    +  +
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVP--------------ATRTYVAPQQPA---YNQDK 120

Query: 181 SPGSLIKVFQVLIPLLILGIAFYMQYYGKK 211
           +P  +IK+ Q L+P+LILG+A  +++Y KK
Sbjct: 121 TPEFIIKILQFLVPILILGLALVVRHYTKK 133

[Close]
BLAST of CmaCh14G021300 vs. TAIR10
Match: AT2G32720.1 (AT2G32720.1 cytochrome B5 isoform B)

HSP 1 Score: 129.8 bits (325), Expect = 2.0e-30
Identity = 68/149 (45.64%), Postives = 93/149 (62.42%), Query Frame = 1 

Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFE 120
           M  + K+F   EV++HN   DCW++I+GKVY+VT  LE+HPGGD+VLL +T +DATDDFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAVANRPPAPQSGTATTAAAKPTAAAPTTTSSAE 180
           +VGHS +A E ME+YYVG +D  T+PK V   PP            +P      T+    
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPP-----------KQPHYNQDKTSE--- 120

Query: 181 SPGSLIKVFQVLIPLLILGIAFYMQYYGK 210
               +IK+ Q L+PL ILG+A  ++ Y K
Sbjct: 121 ---FIIKLLQFLVPLAILGLAVGIRIYTK 132

[Close]
BLAST of CmaCh14G021300 vs. TAIR10
Match: AT2G46650.1 (AT2G46650.1 cytochrome B5 isoform C)

HSP 1 Score: 112.8 bits (281), Expect = 2.5e-25
Identity = 61/141 (43.26%), Postives = 88/141 (62.41%), Query Frame = 1 

Query: 70  FDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFEEVGHSLTAS 129
           F +VAKH  + DCW++I GKVYD+++ ++EHPGGD VLL  T +DA+ DFE+V HS  A 
Sbjct: 7   FHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSKDAK 66

Query: 130 EQMEEYYVGNLDMATLPKAVANRPPAPQSGTATTAAAKPTAAAPTTTSSAESPGSLIKVF 189
           E M++Y +G++D +T+P      PP  +  T          AA TT    E  G  + ++
Sbjct: 67  ELMKKYCIGDVDQSTVPVTQQYIPPWEKEST----------AAETT---KEESGKKLLIY 126

Query: 190 QVLIPLLILGIAFYMQYYGKK 211
             LIPLLILG+AF +++Y  K
Sbjct: 127 --LIPLLILGVAFALRFYNNK 132

[Close]
BLAST of CmaCh14G021300 vs. TAIR10
Match: AT1G26340.1 (AT1G26340.1 cytochrome B5 isoform A)

HSP 1 Score: 111.3 bits (277), Expect = 7.3e-25
Identity = 47/82 (57.32%), Postives = 63/82 (76.83%), Query Frame = 1 

Query: 66  KVFVFDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFEEVGHS 125
           K++  +E A HN Q DCWV+I GKVYDV+S ++EHPGGD+VLL    +DATDDFE+ GHS
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 126 LTASEQMEEYYVGNLDMATLPK 148
             A E ME+Y++G LD ++LP+
Sbjct: 66  KDARELMEKYFIGELDESSLPE 87

[Close]
BLAST of CmaCh14G021300 vs. NCBI nr
Match: gi|449432012|ref|XP_004133794.1| (PREDICTED: cytochrome b5 [Cucumis sativus])

HSP 1 Score: 215.7 bits (548), Expect = 7.8e-53
Identity = 108/151 (71.52%), Postives = 127/151 (84.11%), Query Frame = 1 

Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFE 120
           MASDPK+FVFDEVAKHNHQ DCW+IISGKVYDVT  LE+HPGGDEVLLLATE+DAT+DFE
Sbjct: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAVANRPPAPQSGTATTAAAKPTAAAPTTTSSAE 180
            V HSL A+E+ME+YY+GN+DM+T+PK   +RPPA +S +A       T AA   TSSA+
Sbjct: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAA------TEAATEKTSSAQ 120

Query: 181 SPGSLIKVFQVLIPLLILGIAFYMQYYGKKQ 212
           S GSLIKV QVLIPLLI+G+AFY+QYYGKKQ
Sbjct: 121 SSGSLIKVLQVLIPLLIIGVAFYLQYYGKKQ 145

[Close]
BLAST of CmaCh14G021300 vs. NCBI nr
Match: gi|659074875|ref|XP_008437843.1| (PREDICTED: cytochrome b5-like [Cucumis melo])

HSP 1 Score: 209.9 bits (533), Expect = 4.3e-51
Identity = 108/151 (71.52%), Postives = 128/151 (84.77%), Query Frame = 1 

Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFE 120
           MASDPK+FVFDEVAKHNHQ DCW+IISGKVYDVT  LE+HPGGDEVLLLATE+DAT+DFE
Sbjct: 1   MASDPKLFVFDEVAKHNHQGDCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAVANRPPAPQSGTATTAAAKPTAAAPTTTSSAE 180
            VGHSL+A+E+ME+YY+GN+DM+T+PK V  RPPA +S      A+  T AA  TTSS +
Sbjct: 61  TVGHSLSATEEMEKYYIGNIDMSTVPK-VDYRPPASKS------ASTETKAAAETTSSNQ 120

Query: 181 SPGSLIKVFQVLIPLLILGIAFYMQYYGKKQ 212
           S GSLIKV Q+LIPLLI+ +AFY+QYYGKKQ
Sbjct: 121 SSGSLIKVLQLLIPLLIIVVAFYLQYYGKKQ 144

[Close]
BLAST of CmaCh14G021300 vs. NCBI nr
Match: gi|596010726|ref|XP_007218583.1| (hypothetical protein PRUPE_ppa013234mg [Prunus persica])

HSP 1 Score: 171.8 bits (434), Expect = 1.3e-39
Identity = 86/151 (56.95%), Postives = 110/151 (72.85%), Query Frame = 1 

Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFE 120
           MASD +V+ FDEVAKHNH++DCW+IISGKVYDVT  LE+HPGGDEVLLLA E+DATDDF 
Sbjct: 1   MASDSQVYHFDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKDATDDFN 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAVANRPPAPQSGTATTAAAKPTAAAPTTTSSAE 180
           +VGHS +A EQME++YVG +D +T+P+  + + P                 A TT  S +
Sbjct: 61  DVGHSDSAKEQMEKFYVGKVDTSTIPEQPSYKLP-----------------AQTTPPSGQ 120

Query: 181 SPGSLIKVFQVLIPLLILGIAFYMQYYGKKQ 212
           S G ++K+ Q L+PLLILG AF +QYYGKK+
Sbjct: 121 SSGFVVKLLQFLLPLLILGAAFALQYYGKKK 134

[Close]
BLAST of CmaCh14G021300 vs. NCBI nr
Match: gi|703114550|ref|XP_010100688.1| (Cytochrome b5, seed isoform [Morus notabilis])

HSP 1 Score: 171.4 bits (433), Expect = 1.7e-39
Identity = 89/151 (58.94%), Postives = 110/151 (72.85%), Query Frame = 1 

Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFE 120
           MA D KVF FDEVAKHNH++DCW+IISGKVY+VT  LEEHPGGDEVLLLA E+DATDDFE
Sbjct: 1   MARDSKVFAFDEVAKHNHKKDCWIIISGKVYNVTEFLEEHPGGDEVLLLAAEKDATDDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAVANRPPAPQSGTATTAAAKPTAAAPTTTSSAE 180
           +VGHS TA E ME+YYVG +D++TLP+  + +PP PQ               P + S+  
Sbjct: 61  DVGHSDTAREMMEKYYVGKVDVSTLPEKPSYKPP-PQ---------------PPSQSNQS 120

Query: 181 SPGSLIKVFQVLIPLLILGIAFYMQYYGKKQ 212
           S GS +K+ Q L+PLLIL IAF + +YGK +
Sbjct: 121 SGGS-VKILQFLLPLLILAIAFALHFYGKSK 134

[Close]
BLAST of CmaCh14G021300 vs. NCBI nr
Match: gi|645254539|ref|XP_008233085.1| (PREDICTED: cytochrome b5-like [Prunus mume])

HSP 1 Score: 170.2 bits (430), Expect = 3.7e-39
Identity = 86/151 (56.95%), Postives = 108/151 (71.52%), Query Frame = 1 

Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFE 120
           MASD +V+ FDEVAKHNH++DCW+IISGKVYDVT  LE+HPGGDEVLLLA E+DATDDF 
Sbjct: 1   MASDSQVYHFDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKDATDDFN 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAVANRPPAPQSGTATTAAAKPTAAAPTTTSSAE 180
           +VGHS +A EQME +YVG +D +T+P+    + P                 A TT  S +
Sbjct: 61  DVGHSDSAKEQMENFYVGKVDTSTIPEQPNYKLP-----------------AQTTPPSGQ 120

Query: 181 SPGSLIKVFQVLIPLLILGIAFYMQYYGKKQ 212
           S G ++K+ Q L+PLLILG AF +QYYGKK+
Sbjct: 121 SSGFVVKLLQFLLPLLILGAAFALQYYGKKK 134

The following BLAST results are available for this feature:
BLAST of CmaCh14G021300 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CYB5D_ARATH2.0e-3248.99Cytochrome B5 isoform D OS=Arabidopsis thaliana GN=CYTB5-D PE=1 SV=1[more]
CYB5_BRAOB3.8e-3147.02Cytochrome b5 OS=Brassica oleracea var. botrytis GN=CYB5 PE=1 SV=1[more]
CYB5_TOBAC1.4e-3046.00Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1[more]
CYB5E_ARATH1.9e-3046.00Cytochrome b5 isoform E OS=Arabidopsis thaliana GN=CYTB5-E PE=1 SV=2[more]
CYB5S_TOBAC7.1e-3046.00Cytochrome b5, seed isoform OS=Nicotiana tabacum PE=2 SV=1[more]
BLAST of CmaCh14G021300 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L720_CUCSA5.4e-5371.52Uncharacterized protein OS=Cucumis sativus GN=Csa_3G119260 PE=3 SV=1[more]
M5X125_PRUPE9.0e-4056.95Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013234mg PE=3 SV=1[more]
W9RDL9_9ROSA1.2e-3958.94Cytochrome b5, seed isoform OS=Morus notabilis GN=L484_023457 PE=3 SV=1[more]
A0A151SWJ2_CAJCA1.0e-3552.67Cytochrome b5 OS=Cajanus cajan GN=KK1_014582 PE=3 SV=1[more]
I1NCP5_SOYBN2.3e-3552.32Uncharacterized protein OS=Glycine max GN=GLYMA_19G258500 PE=3 SV=1[more]
BLAST of CmaCh14G021300 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G48810.11.1e-3348.99 cytochrome B5 isoform D[more]
AT5G53560.11.1e-3146.00 cytochrome B5 isoform E[more]
AT2G32720.12.0e-3045.64 cytochrome B5 isoform B[more]
AT2G46650.12.5e-2543.26 cytochrome B5 isoform C[more]
AT1G26340.17.3e-2557.32 cytochrome B5 isoform A[more]
BLAST of CmaCh14G021300 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449432012|ref|XP_004133794.1|7.8e-5371.52PREDICTED: cytochrome b5 [Cucumis sativus][more]
gi|659074875|ref|XP_008437843.1|4.3e-5171.52PREDICTED: cytochrome b5-like [Cucumis melo][more]
gi|596010726|ref|XP_007218583.1|1.3e-3956.95hypothetical protein PRUPE_ppa013234mg [Prunus persica][more]
gi|703114550|ref|XP_010100688.1|1.7e-3958.94Cytochrome b5, seed isoform [Morus notabilis][more]
gi|645254539|ref|XP_008233085.1|3.7e-3956.95PREDICTED: cytochrome b5-like [Prunus mume][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001199Cyt_B5-like_heme/steroid-bd
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0044710 single-organism metabolic process
cellular_component GO:0005575 cellular_component
cellular_component GO:0044444 cytoplasmic part
cellular_component GO:0043231 intracellular membrane-bounded organelle
cellular_component GO:0016020 membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh14G021300.1CmaCh14G021300.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001199Cytochrome b5-like heme/steroid binding domainPRINTSPR00363CYTOCHROMEB5coord: 100..114
score: 6.9E-12coord: 128..140
score: 6.9E-12coord: 115..122
score: 6.9E-12coord: 90..100
score: 6.9
IPR001199Cytochrome b5-like heme/steroid binding domainGENE3DG3DSA:3.10.120.10coord: 65..148
score: 2.1
IPR001199Cytochrome b5-like heme/steroid binding domainPFAMPF00173Cyt-b5coord: 71..140
score: 8.2
IPR001199Cytochrome b5-like heme/steroid binding domainSMARTSM01117Cyt_b5_2coord: 68..141
score: 7.1
IPR001199Cytochrome b5-like heme/steroid binding domainPROFILEPS50255CYTOCHROME_B5_2coord: 65..141
score: 26
IPR001199Cytochrome b5-like heme/steroid binding domainunknownSSF55856Cytochrome b5-like heme/steroid binding domaincoord: 63..147
score: 4.19
NoneNo IPR availablePANTHERPTHR19359CYTOCHROME B5coord: 61..146
score: 4.6E-62coord: 166..211
score: 4.6
NoneNo IPR availablePANTHERPTHR19359:SF22CYTOCHROME B5 ISOFORM Acoord: 166..211
score: 4.6E-62coord: 61..146
score: 4.6

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh14G021300Cucsa.259540Cucumber (Gy14) v1cgycmaB0709
CmaCh14G021300Cla021123Watermelon (97103) v1cmawmB242
CmaCh14G021300Csa3G119260Cucumber (Chinese Long) v2cmacuB253
CmaCh14G021300MELO3C005994Melon (DHL92) v3.5.1cmameB242
CmaCh14G021300ClCG05G000550Watermelon (Charleston Gray)cmawcgB233
CmaCh14G021300CSPI03G07620Wild cucumber (PI 183967)cmacpiB255
CmaCh14G021300CmoCh14G021930Cucurbita moschata (Rifu)cmacmoB245
CmaCh14G021300CmoCh02G011700Cucurbita moschata (Rifu)cmacmoB260
CmaCh14G021300CmoCh06G010160Cucurbita moschata (Rifu)cmacmoB271
CmaCh14G021300Lsi09G000030Bottle gourd (USVL1VR-Ls)cmalsiB226
CmaCh14G021300Lsi05G021600Bottle gourd (USVL1VR-Ls)cmalsiB245
CmaCh14G021300Cp4.1LG05g05960Cucurbita pepo (Zucchini)cmacpeB291
CmaCh14G021300Cp4.1LG03g16000Cucurbita pepo (Zucchini)cmacpeB283
CmaCh14G021300Cp4.1LG08g00630Cucurbita pepo (Zucchini)cmacpeB292
CmaCh14G021300MELO3C005994.2Melon (DHL92) v3.6.1cmamedB273
CmaCh14G021300CsaV3_3G007770Cucumber (Chinese Long) v3cmacucB0291
CmaCh14G021300CsaV3_5G000250Cucumber (Chinese Long) v3cmacucB0305
CmaCh14G021300Cla97C01G000230Watermelon (97103) v2cmawmbB247
CmaCh14G021300Bhi01G000393Wax gourdcmawgoB0315
CmaCh14G021300Bhi12G000587Wax gourdcmawgoB0308
CmaCh14G021300CsGy3G007780Cucumber (Gy14) v2cgybcmaB318
CmaCh14G021300Carg25714Silver-seed gourdcarcmaB0162
CmaCh14G021300Carg20915Silver-seed gourdcarcmaB1373
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh14G021300CmaCh02G011390Cucurbita maxima (Rimu)cmacmaB263
CmaCh14G021300CmaCh06G009870Cucurbita maxima (Rimu)cmacmaB281
The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh14G021300Cucurbita maxima (Rimu)cmacmaB243
CmaCh14G021300Silver-seed gourdcarcmaB1364
CmaCh14G021300Watermelon (97103) v2cmawmbB263