CmaCh02G011390 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh02G011390
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Cytochrome b5-like heme/steroid-binding domain protein
Location	Cma_Chr02 : 6733324 .. 6735930 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAATGAGAAGTTTTACTTGGAAATTTCTAGATTTTGGGGATCCCATTGTTTTGCAAAGAACTTGAATTGCCCTAACAGCCACTTGTTTTGAAGATTCAGGGCGATCTTTCAATTGGGAAATTCCTCATTAAATTACCAGTAAATTGCTTTTTGACTTCGTAATTAACTGCAAATCAAGGGCCATTTTGCCGGCCTCGCTTTTGTTCTTTCATCGTTACTATAAAAAGATTTGTTTTCTCCGTTGCCAAATCGGGAACTAATCTGTAATCAAGCTTCGCCACAAAAAGGATCAGATCAAGACGTTGCCTCTTCAACCCTTTTTCATCTGTGAGTGAATCTGTTCCTTGCGAATCCATTTCGATTTCGAAGGTTTTGATTACTTCTATTGGGGTTTTGGATGTGAAATTTGAAGTTCTTGGCCGCTCCTCTTCGATTGTTTGTTGATTGTTGCAGTGATAAATGGCTTCCGATCCGAAGGTTCATCTGTTCGAAGAAGTGGCAAAGCACAATAAAACGAAGGATTGTTGGCTTGTAATCTCTGGAAAGGTTGAATCTCTCTTGGTTTATTGTTCGTATCGTTTTATTTAGCATTGAATATCAGTTTTCTGGTGATGTTTTTGACGGAAAATTCTTGCGAACTCTGGTTTGTCATCGAATATCCTCTAAAAGAGTTTGATATAGGCGTGTTGGCTTTGGGGGTTTCAGTGTTTTACATGAATGTTCAAATTCAAATATCTTCTTCCTTGTTCTTCACTGAGCCGGATTCGTTTCTATGTCTTAATATCATTTTGTCATAGTAATATCAAATGGGAAGAAAGTGGTGGTGAGCTGCAGCGTATGAAGATTAAATAAAGCAGTGGCATTGTTTTCATATACACTAAAGAGTTTGTCCAACCAATTTTGAGCTTGAAAAACAACATTTATTGGTTGAAAATTGTCCTAAACTTCACTATTTACTATGAGGTTTCAGGCAATTGTTCCTTGTCCCCATAAGAAATCATAGCTTCGTTGAACTGGTTGTTATAAAATGCTTGGAAGGAGTATACCACCCAAGGGGTTGGGCATGGTAGTCTAGACTTGGGGTTAACCAGAGTGGTGTGTTTAAAGCCTGCCTTTTTGGCTACCTTATGAAGATATTGAATTTGTCATATTCATGGTGAATATTGAAAGCTTTGGGGATCAAGCTAAATGTTTAAACTGTTGATAAACGAAGCATTTCTTTTAAGGGTGTGGAAACCTCTCCCTAACATACGTGTTTTAAAACCTTAAGGGGAAACTCAGAAGGGAAAGCCCATAGAGGACAATATCCGCTTGCGATGAGCTTGGGATGTTACAAATGGTATTAGAGTTAGGCTTTGGGTGGTGTGTTAGCGAGGATGTTGGGTCCCTAAGGGGGGTGGATTGTGAGATCTCACGTCGGTTGGAGAGGGGAATGAAGTATTCTTTATAAGGGTGTGGAAACCTCTCCCTAACATACGCGTTTTAAAACCTTGAGGGGATGCCCAAAAGGACAATATCTGCTAGTGGTGAGATTGGGCGGTTACATTCTCGTTTAAATATTTTCGATCATGCATTCAAATTGTGGTGGTTACATTTGTGGAATGTTTAAAATGGTTTGAGATCGTGTTCAAGTTAACATTTATTTAGTTCAATTGGTTAAAATATATGTCGTTGACCAACTAGTCTTAGTTAGAGGTTCATATATTTCCACTCCACTCCACATGTTGAACTCGAAAGGGAAAACAAAGATCACGTATTTGTGAGGTGTTACTAAGCTGGTTTGTTGTGTGTATCATTGATTGTGGCTTTGGGTTTAGGTTTATGATGTAACTCCTTTCATGGAGGACCACCCGGGCGGCGATGAAGTATTGTTGTCTGCAACGGGTAAGCCTCAGAGTACCCTTATATCGAATATATGTATCTGTTATTTTCAAGTCTCTTGCAAATGATGATTAGGATGGATACAATCAATGCTGTTCATTGGAACCGTTTGCAGGAAAAGATGCCTCGAACGATTTCGAGGATGTCGGGCATAGTGATTCAGCAAGAGAAATGATGGACAAATACTACATCGGAGAAATTGATCCTTCAACGGTTCCTTCGAAGAAGATTTACATTCCATCACAACAAACCCAGTACAATCAAGATAAGACGCCCGAGTTTGTGATCAAGATCTTACAGATACTGGTTCCCATATTTATTTTGGGTTTGGCATTTGCGGTTCGACACTACACCAAGAATGAATGAATGAACAAGTGATTATTCGTATCCGTGTCGTTATGTTTGGTAGAAAGGCTGTTTTAGCTTGTTTTTGCTTGTGTTTTCATTGTGGGGGTTAGTCGTAGACTAGAGATGTCTGCATCGGATGTTGTATTCTACTTAATAATAATTAAGCTAAGGACTACCTTTTTCTCCGTTCCATCTTGCTTTGCTTTGTTTTGCTTCTCTTTACTTGTAAACGTTCATCTTTGGAGTTATATTGGCAAGGAATGGAGAGGAGGAATGTCTGGTTTGTCAACAAAAACAAGTCTGTCGGGTTGGTCCAACCATTGCCTATTGAGACTTGAGACTCAAACTCGAAATCAAATATGCAGTACTTACAGAC

mRNA sequence

AAGAATGAGAAGTTTTACTTGGAAATTTCTAGATTTTGGGGATCCCATTGTTTTGCAAAGAACTTGAATTGCCCTAACAGCCACTTGTTTTGAAGATTCAGGGCGATCTTTCAATTGGGAAATTCCTCATTAAATTACCAGTAAATTGCTTTTTGACTTCGTAATTAACTGCAAATCAAGGGCCATTTTGCCGGCCTCGCTTTTGTTCTTTCATCGTTACTATAAAAAGATTTGTTTTCTCCGTTGCCAAATCGGGAACTAATCTGTAATCAAGCTTCGCCACAAAAAGGATCAGATCAAGACGTTGCCTCTTCAACCCTTTTTCATCTTGATAAATGGCTTCCGATCCGAAGGTTCATCTGTTCGAAGAAGTGGCAAAGCACAATAAAACGAAGGATTGTTGGCTTGTAATCTCTGGAAAGGTTTATGATGTAACTCCTTTCATGGAGGACCACCCGGGCGGCGATGAAGTATTGTTGTCTGCAACGGGAAAAGATGCCTCGAACGATTTCGAGGATGTCGGGCATAGTGATTCAGCAAGAGAAATGATGGACAAATACTACATCGGAGAAATTGATCCTTCAACGGTTCCTTCGAAGAAGATTTACATTCCATCACAACAAACCCAGTACAATCAAGATAAGACGCCCGAGTTTGTGATCAAGATCTTACAGATACTGGTTCCCATATTTATTTTGGGTTTGGCATTTGCGGTTCGACACTACACCAAGAATGAATGAATGAACAAGTGATTATTCGTATCCGTGTCGTTATGTTTGGTAGAAAGGCTGTTTTAGCTTGTTTTTGCTTGTGTTTTCATTGTGGGGGTTAGTCGTAGACTAGAGATGTCTGCATCGGATGTTGTATTCTACTTAATAATAATTAAGCTAAGGACTACCTTTTTCTCCGTTCCATCTTGCTTTGCTTTGTTTTGCTTCTCTTTACTTGTAAACGTTCATCTTTGGAGTTATATTGGCAAGGAATGGAGAGGAGGAATGTCTGGTTTGTCAACAAAAACAAGTCTGTCGGGTTGGTCCAACCATTGCCTATTGAGACTTGAGACTCAAACTCGAAATCAAATATGCAGTACTTACAGAC

Coding sequence (CDS)

ATGGCTTCCGATCCGAAGGTTCATCTGTTCGAAGAAGTGGCAAAGCACAATAAAACGAAGGATTGTTGGCTTGTAATCTCTGGAAAGGTTTATGATGTAACTCCTTTCATGGAGGACCACCCGGGCGGCGATGAAGTATTGTTGTCTGCAACGGGAAAAGATGCCTCGAACGATTTCGAGGATGTCGGGCATAGTGATTCAGCAAGAGAAATGATGGACAAATACTACATCGGAGAAATTGATCCTTCAACGGTTCCTTCGAAGAAGATTTACATTCCATCACAACAAACCCAGTACAATCAAGATAAGACGCCCGAGTTTGTGATCAAGATCTTACAGATACTGGTTCCCATATTTATTTTGGGTTTGGCATTTGCGGTTCGACACTACACCAAGAATGAATGA

Protein sequence

MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFEDVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFILGLAFAVRHYTKNE

Homology

[Close]

BLAST of CmaCh02G011390 vs. Swiss-Prot
Match: CYB5E_ARATH (Cytochrome b5 isoform E OS=Arabidopsis thaliana GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 225.3 bits (573), Expect = 3.9e-58
Identity = 104/134 (77.61%), Postives = 119/134 (88.81%), Query Frame = 1


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           M+SD KV  FEEV+KHNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLLS+TGKDA+NDFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSD+AR+MMDKY+IGEID S+VP+ + Y+  QQ  YNQDKTPEF+IKILQ LVPI I
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLA  VRHYTK +
Sbjct: 121 LGLALVVRHYTKKD 134

[Close]

BLAST of CmaCh02G011390 vs. Swiss-Prot
Match: CYB5_BRAOB (Cytochrome b5 OS=Brassica oleracea var. botrytis GN=CYB5 PE=1 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 2.8e-56
Identity = 103/134 (76.87%), Postives = 117/134 (87.31%), Query Frame = 1


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           MAS+ KV  FEEV++HNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLLS+TGKDA+NDFE
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSD+AR+MM+KYYIGEID STVP+ + Y+   Q  YNQDKTPEF+IKILQ LVPI I
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVPATRTYVAPVQPAYNQDKTPEFMIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLA  VR YTK E
Sbjct: 121 LGLALVVRQYTKKE 134

[Close]

BLAST of CmaCh02G011390 vs. Swiss-Prot
Match: CYB5_ORYSJ (Cytochrome b5 OS=Oryza sativa subsp. japonica GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 196.4 bits (498), Expect = 1.9e-49
Identity = 88/129 (68.22%), Postives = 110/129 (85.27%), Query Frame = 1


Query: 6   KVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFEDVGHS 65
           KV+  EEVAKHN   DCWL+I GKVY+V+ F+EDHPGGD+VLLS+TGKDA++DFEDVGHS
Sbjct: 7   KVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFEDVGHS 66

Query: 66  DSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFILGLAF 125
            +AR MMD+YY+G+ID ST+P++  Y+P +Q  YNQDKTPEF+IKILQ LVP+ ILGLA 
Sbjct: 67  TTARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKTPEFIIKILQFLVPLAILGLAV 126

Query: 126 AVRHYTKNE 135
           A+R YTK+E
Sbjct: 127 AIRIYTKSE 135

[Close]

BLAST of CmaCh02G011390 vs. Swiss-Prot
Match: CYB5_TOBAC (Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 4.3e-49
Identity = 88/132 (66.67%), Postives = 107/132 (81.06%), Query Frame = 1


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           M  + KV    EV++HN  KDCWLVISGKVYDVT F++DHPGGDEVLLSATGKDA++DFE
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHS SAR M+D+YY+G+ID +T+P+K  Y P  Q  YNQDKT EFV+K+LQ LVP+ I
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEFVVKLLQFLVPLII 120

Query: 121 LGLAFAVRHYTK 133
           LG+AF +R YTK
Sbjct: 121 LGVAFGIRFYTK 132

[Close]

BLAST of CmaCh02G011390 vs. Swiss-Prot
Match: CYB5B_ARATH (Cytochrome b5 isoform B OS=Arabidopsis thaliana GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 2.8e-48
Identity = 85/133 (63.91%), Postives = 110/133 (82.71%), Query Frame = 1


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           M  + K+    EV++HN+  DCW+VI+GKVY+VT F+EDHPGGD+VLLS+TGKDA++DFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHS+SAREMM++YY+GEIDP+T+P K  Y P +Q  YNQDKT EF+IK+LQ LVP+ I
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAFAVRHYTKN 134
           LGLA  +R YTK+
Sbjct: 121 LGLAVGIRIYTKS 133

[Close]

BLAST of CmaCh02G011390 vs. TrEMBL
Match: A0A0A0KN46_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G139160 PE=3 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 1.4e-67
Identity = 126/134 (94.03%), Postives = 130/134 (97.01%), Query Frame = 1


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDA+NDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVP KKI+IPSQQ+Q+N DKTPEFVIKILQ LVPI I
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of CmaCh02G011390 vs. TrEMBL
Match: A0A0D2R663_GOSRA (Uncharacterized protein (Fragment) OS=Gossypium raimondii GN=B456_008G266200 PE=3 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 8.2e-63
Identity = 115/134 (85.82%), Postives = 124/134 (92.54%), Query Frame = 1


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           MASDPKVH+FEEVA HNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLLSATGKDA++DFE
Sbjct: 31  MASDPKVHIFEEVANHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSATGKDATDDFE 90

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSDSAREMM+KYYIGEIDP TVP+K+ YIP QQT YN DKTPEFVIKILQ LVP+ I
Sbjct: 91  DVGHSDSAREMMEKYYIGEIDPKTVPAKRTYIPPQQTPYNPDKTPEFVIKILQFLVPLLI 150

Query: 121 LGLAFAVRHYTKNE 135
           LGLA AVRHYTK E
Sbjct: 151 LGLAIAVRHYTKKE 164

[Close]

BLAST of CmaCh02G011390 vs. TrEMBL
Match: A0A0D2SS02_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_008G266200 PE=3 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 8.2e-63
Identity = 115/134 (85.82%), Postives = 124/134 (92.54%), Query Frame = 1


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           MASDPKVH+FEEVA HNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLLSATGKDA++DFE
Sbjct: 1   MASDPKVHIFEEVANHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSDSAREMM+KYYIGEIDP TVP+K+ YIP QQT YN DKTPEFVIKILQ LVP+ I
Sbjct: 61  DVGHSDSAREMMEKYYIGEIDPKTVPAKRTYIPPQQTPYNPDKTPEFVIKILQFLVPLLI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLA AVRHYTK E
Sbjct: 121 LGLAIAVRHYTKKE 134

[Close]

BLAST of CmaCh02G011390 vs. TrEMBL
Match: J7FWS2_ROSRU (Yellow stripe-like protein OS=Rosa rugosa PE=4 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 1.1e-62
Identity = 116/134 (86.57%), Postives = 125/134 (93.28%), Query Frame = 1


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           MASDPKVH+FEEVAKHN+TKDCWLVISGKVYDVTPFM+DHPGGDEVLLSATGKDA+NDFE
Sbjct: 699 MASDPKVHVFEEVAKHNQTKDCWLVISGKVYDVTPFMDDHPGGDEVLLSATGKDATNDFE 758

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSD+AR+MMDKYYIGEIDPSTVP K+ YIP  QTQYN DKT EFVIKILQ LVP+ I
Sbjct: 759 DVGHSDAARDMMDKYYIGEIDPSTVPLKRTYIPPPQTQYNPDKTSEFVIKILQFLVPLLI 818

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRH+TK E
Sbjct: 819 LGLAFAVRHFTKKE 832

[Close]

BLAST of CmaCh02G011390 vs. TrEMBL
Match: A0A067DC38_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g032745mg PE=3 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 1.8e-62
Identity = 114/134 (85.07%), Postives = 124/134 (92.54%), Query Frame = 1


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           MASDPK+H FEEVA HNKTKDCWL+ISGKVYDV+ FM+DHPGGDEVL+SATGKDA+NDFE
Sbjct: 1   MASDPKIHQFEEVATHNKTKDCWLIISGKVYDVSSFMDDHPGGDEVLISATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIG+IDPSTVP K+ YIP QQ  YNQDKTPEF+IKILQILVP+ I
Sbjct: 61  DVGHSDSAREMMDKYYIGDIDPSTVPRKRAYIPPQQPAYNQDKTPEFIIKILQILVPLLI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTK E
Sbjct: 121 LGLAFAVRHYTKKE 134

[Close]

BLAST of CmaCh02G011390 vs. TAIR10
Match: AT5G53560.1 (AT5G53560.1 cytochrome B5 isoform E)

HSP 1 Score: 225.3 bits (573), Expect = 2.2e-59
Identity = 104/134 (77.61%), Postives = 119/134 (88.81%), Query Frame = 1


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           M+SD KV  FEEV+KHNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLLS+TGKDA+NDFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSD+AR+MMDKY+IGEID S+VP+ + Y+  QQ  YNQDKTPEF+IKILQ LVPI I
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLA  VRHYTK +
Sbjct: 121 LGLALVVRHYTKKD 134

[Close]

BLAST of CmaCh02G011390 vs. TAIR10
Match: AT2G32720.1 (AT2G32720.1 cytochrome B5 isoform B)

HSP 1 Score: 192.6 bits (488), Expect = 1.6e-49
Identity = 85/133 (63.91%), Postives = 110/133 (82.71%), Query Frame = 1


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           M  + K+    EV++HN+  DCW+VI+GKVY+VT F+EDHPGGD+VLLS+TGKDA++DFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHS+SAREMM++YY+GEIDP+T+P K  Y P +Q  YNQDKT EF+IK+LQ LVP+ I
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAFAVRHYTKN 134
           LGLA  +R YTK+
Sbjct: 121 LGLAVGIRIYTKS 133

[Close]

BLAST of CmaCh02G011390 vs. TAIR10
Match: AT5G48810.1 (AT5G48810.1 cytochrome B5 isoform D)

HSP 1 Score: 177.2 bits (448), Expect = 6.9e-45
Identity = 79/136 (58.09%), Postives = 107/136 (78.68%), Query Frame = 1


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           M  D KV    EV++H+  KDCW+VI GKVYDVT F++DHPGGDEV+L++TGKDA++DFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQ--YNQDKTPEFVIKILQILVPI 120
           DVGHS +A+ M+D+YY+G+ID +TVP K  ++P   T+    QDK+ +FVIK+LQ LVP+
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 121 FILGLAFAVRHYTKNE 135
            ILGLAF +R+YTK +
Sbjct: 121 LILGLAFGIRYYTKTK 136

[Close]

BLAST of CmaCh02G011390 vs. TAIR10
Match: AT2G46650.1 (AT2G46650.1 cytochrome B5 isoform C)

HSP 1 Score: 136.0 bits (341), Expect = 1.8e-32
Identity = 66/123 (53.66%), Postives = 87/123 (70.73%), Query Frame = 1


Query: 10  FEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFEDVGHSDSAR 69
           F +VAKH    DCW++I GKVYD++ FM++HPGGD VLL+ TGKDAS DFEDV HS  A+
Sbjct: 7   FHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSKDAK 66

Query: 70  EMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKT--PEFVIKILQILVPIFILGLAFAV 129
           E+M KY IG++D STVP  + YIP  + +    +T   E   K+L  L+P+ ILG+AFA+
Sbjct: 67  ELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILGVAFAL 126

Query: 130 RHY 131
           R Y
Sbjct: 127 RFY 129

[Close]

BLAST of CmaCh02G011390 vs. TAIR10
Match: AT1G26340.1 (AT1G26340.1 cytochrome B5 isoform A)

HSP 1 Score: 125.6 bits (314), Expect = 2.4e-29
Identity = 61/127 (48.03%), Postives = 89/127 (70.08%), Query Frame = 1


Query: 6   KVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFEDVGHS 65
           K++  EE A HNK  DCW+VI GKVYDV+ +M++HPGGD+VLL+  GKDA++DFED GHS
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 66  DSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQI-----LVPIFI 125
             ARE+M+KY+IGE+D S++P     IP  +  Y +D+  + V K+  +     +VP+ I
Sbjct: 66  KDARELMEKYFIGELDESSLPE----IPELKI-YKKDQPQDSVQKLFDLTKQYWVVPVSI 125

Query: 126 LGLAFAV 128
           + ++ AV
Sbjct: 126 ITISVAV 127

[Close]

BLAST of CmaCh02G011390 vs. NCBI nr
Match: gi|778698736|ref|XP_011654591.1| (PREDICTED: cytochrome b5 isoform E [Cucumis sativus])

HSP 1 Score: 263.5 bits (672), Expect = 2.1e-67
Identity = 126/134 (94.03%), Postives = 130/134 (97.01%), Query Frame = 1


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDA+NDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVP KKI+IPSQQ+Q+N DKTPEFVIKILQ LVPI I
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of CmaCh02G011390 vs. NCBI nr
Match: gi|659074510|ref|XP_008437642.1| (PREDICTED: cytochrome b5 isoform A [Cucumis melo])

HSP 1 Score: 262.3 bits (669), Expect = 4.6e-67
Identity = 126/134 (94.03%), Postives = 129/134 (96.27%), Query Frame = 1


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           MASDPKV LFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDA+NDFE
Sbjct: 1   MASDPKVRLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVP KKIYIPSQQTQ+N DKTPEFVIKILQ LVP+ I
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQHNPDKTPEFVIKILQFLVPMLI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of CmaCh02G011390 vs. NCBI nr
Match: gi|823214401|ref|XP_012439951.1| (PREDICTED: cytochrome b5 isoform E-like [Gossypium raimondii])

HSP 1 Score: 247.7 bits (631), Expect = 1.2e-62
Identity = 115/134 (85.82%), Postives = 124/134 (92.54%), Query Frame = 1


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           MASDPKVH+FEEVA HNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLLSATGKDA++DFE
Sbjct: 1   MASDPKVHIFEEVANHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSDSAREMM+KYYIGEIDP TVP+K+ YIP QQT YN DKTPEFVIKILQ LVP+ I
Sbjct: 61  DVGHSDSAREMMEKYYIGEIDPKTVPAKRTYIPPQQTPYNPDKTPEFVIKILQFLVPLLI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLA AVRHYTK E
Sbjct: 121 LGLAIAVRHYTKKE 134

[Close]

BLAST of CmaCh02G011390 vs. NCBI nr
Match: gi|763779787|gb|KJB46858.1| (hypothetical protein B456_008G266200, partial [Gossypium raimondii])

HSP 1 Score: 247.7 bits (631), Expect = 1.2e-62
Identity = 115/134 (85.82%), Postives = 124/134 (92.54%), Query Frame = 1


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           MASDPKVH+FEEVA HNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLLSATGKDA++DFE
Sbjct: 31  MASDPKVHIFEEVANHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSATGKDATDDFE 90

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSDSAREMM+KYYIGEIDP TVP+K+ YIP QQT YN DKTPEFVIKILQ LVP+ I
Sbjct: 91  DVGHSDSAREMMEKYYIGEIDPKTVPAKRTYIPPQQTPYNPDKTPEFVIKILQFLVPLLI 150

Query: 121 LGLAFAVRHYTKNE 135
           LGLA AVRHYTK E
Sbjct: 151 LGLAIAVRHYTKKE 164

[Close]

BLAST of CmaCh02G011390 vs. NCBI nr
Match: gi|399920236|gb|AFP55583.1| (yellow stripe-like protein [Rosa rugosa])

HSP 1 Score: 247.3 bits (630), Expect = 1.5e-62
Identity = 116/134 (86.57%), Postives = 125/134 (93.28%), Query Frame = 1


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           MASDPKVH+FEEVAKHN+TKDCWLVISGKVYDVTPFM+DHPGGDEVLLSATGKDA+NDFE
Sbjct: 699 MASDPKVHVFEEVAKHNQTKDCWLVISGKVYDVTPFMDDHPGGDEVLLSATGKDATNDFE 758

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSD+AR+MMDKYYIGEIDPSTVP K+ YIP  QTQYN DKT EFVIKILQ LVP+ I
Sbjct: 759 DVGHSDAARDMMDKYYIGEIDPSTVPLKRTYIPPPQTQYNPDKTSEFVIKILQFLVPLLI 818

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRH+TK E
Sbjct: 819 LGLAFAVRHFTKKE 832

The following BLAST results are available for this feature:

BLAST of CmaCh02G011390 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CYB5E_ARATH	3.9e-58	77.61	Cytochrome b5 isoform E OS=Arabidopsis thaliana GN=CYTB5-E PE=1 SV=2	[more]
CYB5_BRAOB	2.8e-56	76.87	Cytochrome b5 OS=Brassica oleracea var. botrytis GN=CYB5 PE=1 SV=1	[more]
CYB5_ORYSJ	1.9e-49	68.22	Cytochrome b5 OS=Oryza sativa subsp. japonica GN=Os05g0108800 PE=2 SV=2	[more]
CYB5_TOBAC	4.3e-49	66.67	Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1	[more]
CYB5B_ARATH	2.8e-48	63.91	Cytochrome b5 isoform B OS=Arabidopsis thaliana GN=CYTB5-B PE=1 SV=1	[more]

BLAST of CmaCh02G011390 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KN46_CUCSA	1.4e-67	94.03	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G139160 PE=3 SV=1	[more]
A0A0D2R663_GOSRA	8.2e-63	85.82	Uncharacterized protein (Fragment) OS=Gossypium raimondii GN=B456_008G266200 PE=...	[more]
A0A0D2SS02_GOSRA	8.2e-63	85.82	Uncharacterized protein OS=Gossypium raimondii GN=B456_008G266200 PE=3 SV=1	[more]
J7FWS2_ROSRU	1.1e-62	86.57	Yellow stripe-like protein OS=Rosa rugosa PE=4 SV=1	[more]
A0A067DC38_CITSI	1.8e-62	85.07	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g032745mg PE=3 SV=1	[more]

BLAST of CmaCh02G011390 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G53560.1	2.2e-59	77.61	cytochrome B5 isoform E	[more]
AT2G32720.1	1.6e-49	63.91	cytochrome B5 isoform B	[more]
AT5G48810.1	6.9e-45	58.09	cytochrome B5 isoform D	[more]
AT2G46650.1	1.8e-32	53.66	cytochrome B5 isoform C	[more]
AT1G26340.1	2.4e-29	48.03	cytochrome B5 isoform A	[more]

BLAST of CmaCh02G011390 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778698736\|ref\|XP_011654591.1\|	2.1e-67	94.03	PREDICTED: cytochrome b5 isoform E [Cucumis sativus]	[more]
gi\|659074510\|ref\|XP_008437642.1\|	4.6e-67	94.03	PREDICTED: cytochrome b5 isoform A [Cucumis melo]	[more]
gi\|823214401\|ref\|XP_012439951.1\|	1.2e-62	85.82	PREDICTED: cytochrome b5 isoform E-like [Gossypium raimondii]	[more]
gi\|763779787\|gb\|KJB46858.1\|	1.2e-62	85.82	hypothetical protein B456_008G266200, partial [Gossypium raimondii]	[more]
gi\|399920236\|gb\|AFP55583.1\|	1.5e-62	86.57	yellow stripe-like protein [Rosa rugosa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001199	Cyt_B5-like_heme/steroid-bd
IPR018506	Cyt_B5_heme-BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006040	amino sugar metabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0019745	pentacyclic triterpenoid biosynthetic process
biological_process	GO:0016126	sterol biosynthetic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0009535	chloroplast thylakoid membrane
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0005774	vacuolar membrane
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004128	cytochrome-b5 reductase activity, acting on NAD(P)H
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh02G011390.1	CmaCh02G011390.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 30..40 score: 1.5E-15coord: 40..54 score: 1.5E-15coord: 55..62 score: 1.5E-15coord: 68..80 score: 1.5
IPR001199	Cytochrome b5-like heme/steroid binding domain	GENE3D	G3DSA:3.10.120.10		coord: 5..87 score: 1.2
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 11..80 score: 9.8
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 score: 3.6
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROFILE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 29
IPR001199	Cytochrome b5-like heme/steroid binding domain	unknown	SSF55856	Cytochrome b5-like heme/steroid binding domain	coord: 4..86 score: 2.22
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43 scor
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..134 score: 1.7E
None	No IPR available	PANTHER	PTHR19359:SF22	CYTOCHROME B5 ISOFORM A	coord: 1..134 score: 1.7E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh02G011390	Cucsa.202340	Cucumber (Gy14) v1	cgycmaB0602
CmaCh02G011390	Cla004873	Watermelon (97103) v1	cmawmB574
CmaCh02G011390	Csa5G139160	Cucumber (Chinese Long) v2	cmacuB612
CmaCh02G011390	MELO3C005907	Melon (DHL92) v3.5.1	cmameB527
CmaCh02G011390	ClCG01G000210	Watermelon (Charleston Gray)	cmawcgB523
CmaCh02G011390	ClCG05G000550	Watermelon (Charleston Gray)	cmawcgB563
CmaCh02G011390	CmoCh02G011700	Cucurbita moschata (Rifu)	cmacmoB619
CmaCh02G011390	CmoCh15G010730	Cucurbita moschata (Rifu)	cmacmoB584
CmaCh02G011390	Lsi09G000030	Bottle gourd (USVL1VR-Ls)	cmalsiB536
CmaCh02G011390	Cp4.1LG13g03230	Cucurbita pepo (Zucchini)	cmacpeB602
CmaCh02G011390	Cp4.1LG05g05960	Cucurbita pepo (Zucchini)	cmacpeB646
CmaCh02G011390	MELO3C005907.2	Melon (DHL92) v3.6.1	cmamedB605
CmaCh02G011390	MELO3C005994.2	Melon (DHL92) v3.6.1	cmamedB664
CmaCh02G011390	CsaV3_3G007770	Cucumber (Chinese Long) v3	cmacucB0721
CmaCh02G011390	CsaV3_5G000250	Cucumber (Chinese Long) v3	cmacucB0735
CmaCh02G011390	Cla97C01G000230	Watermelon (97103) v2	cmawmbB604
CmaCh02G011390	Bhi12G000587	Wax gourd	cmawgoB0710
CmaCh02G011390	CsGy5G000190	Cucumber (Gy14) v2	cgybcmaB639
CmaCh02G011390	CsGy3G007780	Cucumber (Gy14) v2	cgybcmaB401
CmaCh02G011390	Carg26776	Silver-seed gourd	carcmaB0808
CmaCh02G011390	Carg13998	Silver-seed gourd	carcmaB0716

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
CmaCh02G011390	CmaCh14G021300	Cucurbita maxima (Rimu)	cmacmaB263
CmaCh02G011390	CmaCh15G010100	Cucurbita maxima (Rimu)	cmacmaB313

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh02G011390	Watermelon (97103) v2	cmawmbB649
CmaCh02G011390	Wax gourd	cmawgoB0727
CmaCh02G011390	Cucurbita maxima (Rimu)	cmacmaB346
CmaCh02G011390	Cucurbita maxima (Rimu)	cmacmaB396
CmaCh02G011390	Cucurbita moschata (Rifu)	cmacmoB587
CmaCh02G011390	Cucurbita moschata (Rifu)	cmacmoB591
CmaCh02G011390	Wild cucumber (PI 183967)	cmacpiB618
CmaCh02G011390	Melon (DHL92) v3.5.1	cmameB579
CmaCh02G011390	Watermelon (Charleston Gray)	cmawcgB559
CmaCh02G011390	Watermelon (97103) v1	cmawmB564
CmaCh02G011390	Cucurbita pepo (Zucchini)	cmacpeB598
CmaCh02G011390	Cucurbita pepo (Zucchini)	cmacpeB608
CmaCh02G011390	Cucurbita pepo (Zucchini)	cmacpeB639
CmaCh02G011390	Bottle gourd (USVL1VR-Ls)	cmalsiB584
CmaCh02G011390	Cucumber (Gy14) v2	cgybcmaB644
CmaCh02G011390	Silver-seed gourd	carcmaB0756
CmaCh02G011390	Silver-seed gourd	carcmaB1369
CmaCh02G011390	Cucumber (Chinese Long) v3	cmacucB0723