Cucsa.202340 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.202340
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionCytochrome b5
Locationscaffold01376 : 391689 .. 394452 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGCAGTTGAAGAGGTGGAGAAGGTGAGATGTTTAGAGATAGATTCGTGAGTTCTATCTCCAATCCAAAAGTCTTTTCTTGTTATGTACTATCTCTAGTCCATAATTTAAGTTTAGGGTATTCTGTGGGTATCTCTTCAACCTATTAACTCTTTGTGGGTTTTTGTTTTTAACTCAGATCATGTAATAGTTTTGATTTGGAAATGTTTTCTTCAGCGGATTGTTTCACAAACTCAAATGTTAAAATTTAACTAGGGTTTATTCCAACTATTATTTTCCTACATCTTTTTCTTATGCATCGTTTTATTGTTATGTGTTGTTGAAATCTATTTATTTTGTAATTCATTGTTATTCCAATTCAATTATTCAATAATATTATACAAATTTGATTTAACAATGAAAATTTTGAGAAGAAAGAGTTGCAATAAAATGATTATAGATGGTGAAAAATAAGAGTAAAGCAAGAGTTAAAAGAAAAAGAATAAGGTAGAAGACAAAGTTGGAGAATGGTATCCAACTTGAACATCCTCTCTTTTGGTTTAAAATGTTTGGTAGAACTTAGAAGGAGTTTTCCAATGTAATGTGAAACCCATAGGGCATAGGGGTGATTGAGTCCCACACCACATGTTTTGATGTTTTAAAACTTCGATTAGGAAATTCCCCATTAATTACAAGTAAAATGCTTCCCCACTTACCTTTTTCACCTTTTTGCCGCCCTTTATATACCTTAATTAGAATTAATAAGAATTGAAAAGCAAAAATCAATTCTTCCAACTTAAGATCAAGAACCCATCTGTGTTCTGTAATCGCTCGGTCTTTGCCGCAAACAAGGATCCGAACTACACGTTCCCTCTCACCCATTTCCCAGCTGTAGGTTTCCTTCTTTCTTACCCACCCATTTCAATTTCTGTTGTTTTCTTCTGCTCTCTCGCTCTCTTTCTTTCCAATGCCTCTATACTTGCTTTTGGATCCCTTTTCTTTCTGGGGGTTTCTTAATTTTGGTTTTTGCAGGCATAAATGGCTTCGGATCCGAAGGTTCATCTTTTCGAAGAAGTGGCAAAGCACAACAAAACCAAAGATTGCTGGCTTGTTATCTCTGGGAAGGTTGGATCTCTCTTACTTGATTCTTCTTATTCATCACTAGATCGATCAGTTCTGGTTTAATTGTTGACTATTTCTTATTTGGTTGGCAAAAAAAGCATGTCCTATGTTTTTGGACGAGGAAAACCAAAAGCCCTTGCTAAAGAAAAACATATGGCTGTGGCTAAGGGCTTTAACTCTTTCCTAACTCTGGATTGTGGATTCTCATCAAATATCCACTAAAAGAGTTTTTGATATAGATGCAGTAGCTTTGGGGGTTCAATTCCTTTGCATGCACGTTCTCATATTTTTGTTCACAGATACTCAAATTCGAATATCTTCTTCCTTTTTCTTCTTTGTTTTGATTCATATAATAGATTTTTtCTTCTGTATACCTTTAATACAAAAGATAATTTAGTCTCATTTCCTTCTGCTCTGTTTGTATTGTCCTGATTTATTTGGTATGCCGCTTATATGTAGTGTACGAAGGCTATATAAAGGTCTGACATTGACTAAAGAGTCTTAAGATTTTTTTtCTTCTCCCCATAAGAAATCATAACTTCCATTGAGTTGCTTGTTATATTATGCTTAGAAGTATACCCATCCCAAGGGTTGGCATGCTGTCGAAGAATTGGGGTTAACCAAAATGGTGATTGGTTTAAACCCCTTTATTGGCTACCTTGTAGAGATATTAAATTTGTCACATTCTTGGTGAATGTCGAGAGCTTTGGGTACCAAACTGAGTTTAAAATAGTGATAATTAGAGATGAGGTAATTTAGAAATTCTTTACCAGCTCTCGTTAAGATATTTTGAGCCCCAACCACTATGGTTTGGGGTTGACTCAATATTAAAAAAACCACTTAAATTTTCCAGAACCAAATGCCTTAGGGACCATTTGTTCAAAATGACGAGATACTAAGCTGGCTTGTATGAGTAGTTTATTTTCAGTGGAAGTAGTGAGATTTCTTTTCTCATGTTTGATTGCTTATGGCCTTTACTTTAGGTTTATGATGTAACTCCTTTCATGGAGGATCATCCCGGAGGTGATGAAGTATTATTGTCAGCAACTGGTAATCCTCGGAATTCCCTTATAATAACAAACACTTGATTCTGTTTCTTTGAGGTCATTGCGAATGATGATTAGGATGGATATGATAACAGTTACTAATTGTGTTTTGCAGGAAAAGATGCCACAAACGATTTCGAAGATGTTGGGCACAGCGATTCAGCAAGAGAAATGATGGACAAATATTACATCGGAGAAATTGATCCTTCAACGGTTCCTTTGAAGAAGATTTTCATTCCATCACAACAATCACAGCACAACCCAGATAAGACACCTGAGTTTGTGATCAAGATCTTGCAGTTTTTGGTTCCCATTTTGATTTTGGGTTTGGCATTTGCTGTTCGACACTACACCAAGAATGAATAAATAAAACCCTCTGGAGGGTAAACTGCATATTCATATCGATTATATTTGGTAGAAAGAACGTTCTCTTTTTCTTCTTTATCTTCTTGTCATGTGATTTCATGTCGGGGTTGGTCGTAGACTAGAGTTCTCTCATCGGTTTTTGTATTCTCCTTAATAATAAGTAAAGCTATGTGCTATCTTTTCTTTTCTCCTTTTCCCCCcTTCCTTTATTGCTCTGCTCGTCTCTTTAATGATAAATCAACCCTATTTTGTT

mRNA sequence

CGCAGTTGAAGAGGTGGAGAAGGTGAGATGTTTAGAGATAGATTCGTGAGTTCTATCTCCAATCCAAAAGTCTTTTCTTGTTATGTACTATCTCTAGTCCATAATTTAAGTTTAGGGTATTCTGTGGGTATCTCTTCAACCTATTAACTCTTTGTGGGTTTTTGTTTTTAACTCAGATCATGTAATAGTTTTGATTTGGAAATGTTTTCTTCAGCGGATTGTTTCACAAACTCAAATGTTAAAATTTAACTAGGGTTTATTCCAACTATTATTTTCCTACATCTTTTTCTTATGCATCGTTTTATTGTTATGTGTTGTTGAAATCTATTTATTTTGTAATTCATTGTTATTCCAATTCAATTATTCAATAATATTATACAAATTTGATTTAACAATGAAAATTTTGAGAAGAAAGAGTTGCAATAAAATGATTATAGATGGTGAAAAATAAGAGTAAAGCAAGAGTTAAAAGAAAAAGAATAAGGTAGAAGACAAAGTTGGAGAATGGTATCCAACTTGAACATCCTCTCTTTTGGTTTAAAATGTTTGGTAGAACTTAGAAGGAGTTTTCCAATGTAATGTGAAACCCATAGGGCATAGGGGTGATTGAGTCCCACACCACATGTTTTGATGTTTTAAAACTTCGATTAGGAAATTCCCCATTAATTACAAGTAAAATGCTTCCCCACTTACCTTTTTCACCTTTTTGCCGCCCTTTATATACCTTAATTAGAATTAATAAGAATTGAAAAGCAAAAATCAATTCTTCCAACTTAAGATCAAGAACCCATCTGTGTTCTGTAATCGCTCGGTCTTTGCCGCAAACAAGGATCCGAACTACACGTTCCCTCTCACCCATTTCCCAGCTGCATAAATGGCTTCGGATCCGAAGGTTCATCTTTTCGAAGAAGTGGCAAAGCACAACAAAACCAAAGATTGCTGGCTTGTTATCTCTGGGAAGGTTTATGATGTAACTCCTTTCATGGAGGATCATCCCGGAGGTGATGAAGTATTATTGTCAGCAACTGGAAAAGATGCCACAAACGATTTCGAAGATGTTGGGCACAGCGATTCAGCAAGAGAAATGATGGACAAATATTACATCGGAGAAATTGATCCTTCAACGGTTCCTTTGAAGAAGATTTTCATTCCATCACAACAATCACAGCACAACCCAGATAAGACACCTGAGTTTGTGATCAAGATCTTGCAGTTTTTGGTTCCCATTTTGATTTTGGGTTTGGCATTTGCTGTTCGACACTACACCAAGAATGAATAAATAAAACCCTCTGGAGGGTAAACTGCATATTCATATCGATTATATTTGGTAGAAAGAACGTTCTCTTTTTCTTCTTTATCTTCTTGTCATGTGATTTCATGTCGGGGTTGGTCGTAGACTAGAGTTCTCTCATCGGTTTTTGTATTCTCCTTAATAATAAGTAAAGCTATGTGCTATCTTTTCTTTTCTCCTTTTCCCCCCTTCCTTTATTGCTCTGCTCGTCTCTTTAATGATAAATCAACCCTATTTTGTT

Coding sequence (CDS)

ATGGCTTCGGATCCGAAGGTTCATCTTTTCGAAGAAGTGGCAAAGCACAACAAAACCAAAGATTGCTGGCTTGTTATCTCTGGGAAGGTTTATGATGTAACTCCTTTCATGGAGGATCATCCCGGAGGTGATGAAGTATTATTGTCAGCAACTGGAAAAGATGCCACAAACGATTTCGAAGATGTTGGGCACAGCGATTCAGCAAGAGAAATGATGGACAAATATTACATCGGAGAAATTGATCCTTCAACGGTTCCTTTGAAGAAGATTTTCATTCCATCACAACAATCACAGCACAACCCAGATAAGACACCTGAGTTTGTGATCAAGATCTTGCAGTTTTTGGTTCCCATTTTGATTTTGGGTTTGGCATTTGCTGTTCGACACTACACCAAGAATGAATAA

Protein sequence

MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILILGLAFAVRHYTKNE*
Homology
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BLAST of Cucsa.202340 vs. Swiss-Prot
Match: CYB5E_ARATH (Cytochrome b5 isoform E OS=Arabidopsis thaliana GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 223.0 bits (567), Expect = 2.0e-57
Identity = 104/134 (77.61%), Postives = 119/134 (88.81%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           M+SD KV  FEEV+KHNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLLS+TGKDATNDFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120
           DVGHSD+AR+MMDKY+IGEID S+VP  + ++  QQ  +N DKTPEF+IKILQFLVPILI
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLA  VRHYTK +
Sbjct: 121 LGLALVVRHYTKKD 134

[Close]
BLAST of Cucsa.202340 vs. Swiss-Prot
Match: CYB5_BRAOB (Cytochrome b5 OS=Brassica oleracea var. botrytis GN=CYB5 PE=1 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 1.4e-55
Identity = 103/134 (76.87%), Postives = 117/134 (87.31%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           MAS+ KV  FEEV++HNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLLS+TGKDATNDFE
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120
           DVGHSD+AR+MM+KYYIGEID STVP  + ++   Q  +N DKTPEF+IKILQFLVPILI
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVPATRTYVAPVQPAYNQDKTPEFMIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLA  VR YTK E
Sbjct: 121 LGLALVVRQYTKKE 134

[Close]
BLAST of Cucsa.202340 vs. Swiss-Prot
Match: CYB5_ORYSJ (Cytochrome b5 OS=Oryza sativa subsp. japonica GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 193.4 bits (490), Expect = 1.7e-48
Identity = 87/129 (67.44%), Postives = 109/129 (84.50%), Query Frame = 1 

Query: 6   KVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFEDVGHS 65
           KV+  EEVAKHN   DCWL+I GKVY+V+ F+EDHPGGD+VLLS+TGKDAT+DFEDVGHS
Sbjct: 7   KVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFEDVGHS 66

Query: 66  DSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILILGLAF 125
            +AR MMD+YY+G+ID ST+P +  ++P +Q  +N DKTPEF+IKILQFLVP+ ILGLA 
Sbjct: 67  TTARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKTPEFIIKILQFLVPLAILGLAV 126

Query: 126 AVRHYTKNE 135
           A+R YTK+E
Sbjct: 127 AIRIYTKSE 135

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BLAST of Cucsa.202340 vs. Swiss-Prot
Match: CYB5_TOBAC (Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 2.2e-48
Identity = 87/132 (65.91%), Postives = 107/132 (81.06%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           M  + KV    EV++HN  KDCWLVISGKVYDVT F++DHPGGDEVLLSATGKDAT+DFE
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120
           DVGHS SAR M+D+YY+G+ID +T+P K  + P  Q  +N DKT EFV+K+LQFLVP++I
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEFVVKLLQFLVPLII 120

Query: 121 LGLAFAVRHYTK 133
           LG+AF +R YTK
Sbjct: 121 LGVAFGIRFYTK 132

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BLAST of Cucsa.202340 vs. Swiss-Prot
Match: CYB5B_ARATH (Cytochrome b5 isoform B OS=Arabidopsis thaliana GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 2.4e-47
Identity = 84/133 (63.16%), Postives = 110/133 (82.71%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           M  + K+    EV++HN+  DCW+VI+GKVY+VT F+EDHPGGD+VLLS+TGKDAT+DFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120
           DVGHS+SAREMM++YY+GEIDP+T+P K  + P +Q  +N DKT EF+IK+LQFLVP+ I
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAFAVRHYTKN 134
           LGLA  +R YTK+
Sbjct: 121 LGLAVGIRIYTKS 133

[Close]
BLAST of Cucsa.202340 vs. TrEMBL
Match: A0A0A0KN46_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G139160 PE=3 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 7.4e-72
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]
BLAST of Cucsa.202340 vs. TrEMBL
Match: J7FWS2_ROSRU (Yellow stripe-like protein OS=Rosa rugosa PE=4 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 4.4e-64
Identity = 118/134 (88.06%), Postives = 129/134 (96.27%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           MASDPKVH+FEEVAKHN+TKDCWLVISGKVYDVTPFM+DHPGGDEVLLSATGKDATNDFE
Sbjct: 699 MASDPKVHVFEEVAKHNQTKDCWLVISGKVYDVTPFMDDHPGGDEVLLSATGKDATNDFE 758

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120
           DVGHSD+AR+MMDKYYIGEIDPSTVPLK+ +IP  Q+Q+NPDKT EFVIKILQFLVP+LI
Sbjct: 759 DVGHSDAARDMMDKYYIGEIDPSTVPLKRTYIPPPQTQYNPDKTSEFVIKILQFLVPLLI 818

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRH+TK E
Sbjct: 819 LGLAFAVRHFTKKE 832

[Close]
BLAST of Cucsa.202340 vs. TrEMBL
Match: M5X2R7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013232mg PE=3 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 7.5e-64
Identity = 117/134 (87.31%), Postives = 129/134 (96.27%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           MASDPKVH+FEEVAKHNKTKDCWLVISGKV+DVTPFM+DHPGGDEVLLSATGKDATNDFE
Sbjct: 1   MASDPKVHIFEEVAKHNKTKDCWLVISGKVFDVTPFMDDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120
           DVGHSDSAR+MM+KYYIGE+DPSTVPLK+I+IP  Q+++NPDK  EFVIKILQFLVP+LI
Sbjct: 61  DVGHSDSARDMMEKYYIGEVDPSTVPLKRIYIPPPQARYNPDKISEFVIKILQFLVPLLI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTK E
Sbjct: 121 LGLAFAVRHYTKKE 134

[Close]
BLAST of Cucsa.202340 vs. TrEMBL
Match: K4BI36_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=3 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 1.3e-63
Identity = 117/134 (87.31%), Postives = 127/134 (94.78%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           MASD K+H FEEVAKHNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLLSATGKDATNDFE
Sbjct: 57  MASDRKIHAFEEVAKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSATGKDATNDFE 116

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120
           DVGHSDSAREMMDKYYIGEID STVPLK+ +IPS+Q+ +NPDKTPEF+IKILQFLVPILI
Sbjct: 117 DVGHSDSAREMMDKYYIGEIDMSTVPLKRAYIPSEQTAYNPDKTPEFIIKILQFLVPILI 176

Query: 121 LGLAFAVRHYTKNE 135
           LGLAF VRHYTK +
Sbjct: 177 LGLAFTVRHYTKEQ 190

[Close]
BLAST of Cucsa.202340 vs. TrEMBL
Match: A0A0D2SS02_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_008G266200 PE=3 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 2.8e-63
Identity = 116/134 (86.57%), Postives = 126/134 (94.03%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           MASDPKVH+FEEVA HNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLLSATGKDAT+DFE
Sbjct: 1   MASDPKVHIFEEVANHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120
           DVGHSDSAREMM+KYYIGEIDP TVP K+ +IP QQ+ +NPDKTPEFVIKILQFLVP+LI
Sbjct: 61  DVGHSDSAREMMEKYYIGEIDPKTVPAKRTYIPPQQTPYNPDKTPEFVIKILQFLVPLLI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLA AVRHYTK E
Sbjct: 121 LGLAIAVRHYTKKE 134

[Close]
BLAST of Cucsa.202340 vs. TAIR10
Match: AT5G53560.1 (AT5G53560.1 cytochrome B5 isoform E)

HSP 1 Score: 223.0 bits (567), Expect = 1.1e-58
Identity = 104/134 (77.61%), Postives = 119/134 (88.81%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           M+SD KV  FEEV+KHNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLLS+TGKDATNDFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120
           DVGHSD+AR+MMDKY+IGEID S+VP  + ++  QQ  +N DKTPEF+IKILQFLVPILI
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLA  VRHYTK +
Sbjct: 121 LGLALVVRHYTKKD 134

[Close]
BLAST of Cucsa.202340 vs. TAIR10
Match: AT2G32720.1 (AT2G32720.1 cytochrome B5 isoform B)

HSP 1 Score: 189.5 bits (480), Expect = 1.3e-48
Identity = 84/133 (63.16%), Postives = 110/133 (82.71%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           M  + K+    EV++HN+  DCW+VI+GKVY+VT F+EDHPGGD+VLLS+TGKDAT+DFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120
           DVGHS+SAREMM++YY+GEIDP+T+P K  + P +Q  +N DKT EF+IK+LQFLVP+ I
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAFAVRHYTKN 134
           LGLA  +R YTK+
Sbjct: 121 LGLAVGIRIYTKS 133

[Close]
BLAST of Cucsa.202340 vs. TAIR10
Match: AT5G48810.1 (AT5G48810.1 cytochrome B5 isoform D)

HSP 1 Score: 181.0 bits (458), Expect = 4.8e-46
Identity = 82/136 (60.29%), Postives = 110/136 (80.88%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           M  D KV    EV++H+  KDCW+VI GKVYDVT F++DHPGGDEV+L++TGKDAT+DFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIP--SQQSQHNPDKTPEFVIKILQFLVPI 120
           DVGHS +A+ M+D+YY+G+ID +TVP+K  F+P  S ++    DK+ +FVIK+LQFLVP+
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 121 LILGLAFAVRHYTKNE 135
           LILGLAF +R+YTK +
Sbjct: 121 LILGLAFGIRYYTKTK 136

[Close]
BLAST of Cucsa.202340 vs. TAIR10
Match: AT2G46650.1 (AT2G46650.1 cytochrome B5 isoform C)

HSP 1 Score: 138.7 bits (348), Expect = 2.7e-33
Identity = 65/123 (52.85%), Postives = 90/123 (73.17%), Query Frame = 1 

Query: 10  FEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFEDVGHSDSAR 69
           F +VAKH    DCW++I GKVYD++ FM++HPGGD VLL+ TGKDA+ DFEDV HS  A+
Sbjct: 7   FHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSKDAK 66

Query: 70  EMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKT--PEFVIKILQFLVPILILGLAFAV 129
           E+M KY IG++D STVP+ + +IP  + +    +T   E   K+L +L+P+LILG+AFA+
Sbjct: 67  ELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILGVAFAL 126

Query: 130 RHY 131
           R Y
Sbjct: 127 RFY 129

[Close]
BLAST of Cucsa.202340 vs. TAIR10
Match: AT1G26340.1 (AT1G26340.1 cytochrome B5 isoform A)

HSP 1 Score: 123.6 bits (309), Expect = 9.1e-29
Identity = 62/125 (49.60%), Postives = 87/125 (69.60%), Query Frame = 1 

Query: 6   KVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFEDVGHS 65
           K++  EE A HNK  DCW+VI GKVYDV+ +M++HPGGD+VLL+  GKDAT+DFED GHS
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 66  DSAREMMDKYYIGEIDPST---VPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILILG 125
             ARE+M+KY+IGE+D S+   +P  KI+    Q Q +  K   F +    ++VP+ I+ 
Sbjct: 66  KDARELMEKYFIGELDESSLPEIPELKIY-KKDQPQDSVQKL--FDLTKQYWVVPVSIIT 125

Query: 126 LAFAV 128
           ++ AV
Sbjct: 126 ISVAV 127

[Close]
BLAST of Cucsa.202340 vs. NCBI nr
Match: gi|778698736|ref|XP_011654591.1| (PREDICTED: cytochrome b5 isoform E [Cucumis sativus])

HSP 1 Score: 277.7 bits (709), Expect = 1.1e-71
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]
BLAST of Cucsa.202340 vs. NCBI nr
Match: gi|659074510|ref|XP_008437642.1| (PREDICTED: cytochrome b5 isoform A [Cucumis melo])

HSP 1 Score: 271.2 bits (692), Expect = 1.0e-69
Identity = 130/134 (97.01%), Postives = 133/134 (99.25%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           MASDPKV LFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE
Sbjct: 1   MASDPKVRLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPLKKI+IPSQQ+QHNPDKTPEFVIKILQFLVP+LI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQHNPDKTPEFVIKILQFLVPMLI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]
BLAST of Cucsa.202340 vs. NCBI nr
Match: gi|399920236|gb|AFP55583.1| (yellow stripe-like protein [Rosa rugosa])

HSP 1 Score: 251.9 bits (642), Expect = 6.3e-64
Identity = 118/134 (88.06%), Postives = 129/134 (96.27%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           MASDPKVH+FEEVAKHN+TKDCWLVISGKVYDVTPFM+DHPGGDEVLLSATGKDATNDFE
Sbjct: 699 MASDPKVHVFEEVAKHNQTKDCWLVISGKVYDVTPFMDDHPGGDEVLLSATGKDATNDFE 758

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120
           DVGHSD+AR+MMDKYYIGEIDPSTVPLK+ +IP  Q+Q+NPDKT EFVIKILQFLVP+LI
Sbjct: 759 DVGHSDAARDMMDKYYIGEIDPSTVPLKRTYIPPPQTQYNPDKTSEFVIKILQFLVPLLI 818

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRH+TK E
Sbjct: 819 LGLAFAVRHFTKKE 832

[Close]
BLAST of Cucsa.202340 vs. NCBI nr
Match: gi|747075053|ref|XP_011084546.1| (PREDICTED: cytochrome b5 isoform E-like [Sesamum indicum])

HSP 1 Score: 251.5 bits (641), Expect = 8.2e-64
Identity = 118/134 (88.06%), Postives = 127/134 (94.78%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           MASDPKVH FEEVAKHNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLLSATGKDATNDFE
Sbjct: 1   MASDPKVHTFEEVAKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120
           DVGHSDSAREMMDKYYIGEID +TVPLK+ + P QQ+ +NPDKTPEFVIKILQFLVP+LI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDMATVPLKRAYTPPQQAHYNPDKTPEFVIKILQFLVPLLI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTK +
Sbjct: 121 LGLAFAVRHYTKEK 134

[Close]
BLAST of Cucsa.202340 vs. NCBI nr
Match: gi|596010715|ref|XP_007218582.1| (hypothetical protein PRUPE_ppa013232mg [Prunus persica])

HSP 1 Score: 251.1 bits (640), Expect = 1.1e-63
Identity = 117/134 (87.31%), Postives = 129/134 (96.27%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60
           MASDPKVH+FEEVAKHNKTKDCWLVISGKV+DVTPFM+DHPGGDEVLLSATGKDATNDFE
Sbjct: 1   MASDPKVHIFEEVAKHNKTKDCWLVISGKVFDVTPFMDDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120
           DVGHSDSAR+MM+KYYIGE+DPSTVPLK+I+IP  Q+++NPDK  EFVIKILQFLVP+LI
Sbjct: 61  DVGHSDSARDMMEKYYIGEVDPSTVPLKRIYIPPPQARYNPDKISEFVIKILQFLVPLLI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTK E
Sbjct: 121 LGLAFAVRHYTKKE 134

The following BLAST results are available for this feature:
BLAST of Cucsa.202340 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CYB5E_ARATH2.0e-5777.61Cytochrome b5 isoform E OS=Arabidopsis thaliana GN=CYTB5-E PE=1 SV=2[more]
CYB5_BRAOB1.4e-5576.87Cytochrome b5 OS=Brassica oleracea var. botrytis GN=CYB5 PE=1 SV=1[more]
CYB5_ORYSJ1.7e-4867.44Cytochrome b5 OS=Oryza sativa subsp. japonica GN=Os05g0108800 PE=2 SV=2[more]
CYB5_TOBAC2.2e-4865.91Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1[more]
CYB5B_ARATH2.4e-4763.16Cytochrome b5 isoform B OS=Arabidopsis thaliana GN=CYTB5-B PE=1 SV=1[more]
BLAST of Cucsa.202340 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KN46_CUCSA7.4e-72100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_5G139160 PE=3 SV=1[more]
J7FWS2_ROSRU4.4e-6488.06Yellow stripe-like protein OS=Rosa rugosa PE=4 SV=1[more]
M5X2R7_PRUPE7.5e-6487.31Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013232mg PE=3 SV=1[more]
K4BI36_SOLLC1.3e-6387.31Uncharacterized protein OS=Solanum lycopersicum PE=3 SV=1[more]
A0A0D2SS02_GOSRA2.8e-6386.57Uncharacterized protein OS=Gossypium raimondii GN=B456_008G266200 PE=3 SV=1[more]
BLAST of Cucsa.202340 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G53560.11.1e-5877.61 cytochrome B5 isoform E[more]
AT2G32720.11.3e-4863.16 cytochrome B5 isoform B[more]
AT5G48810.14.8e-4660.29 cytochrome B5 isoform D[more]
AT2G46650.12.7e-3352.85 cytochrome B5 isoform C[more]
AT1G26340.19.1e-2949.60 cytochrome B5 isoform A[more]
BLAST of Cucsa.202340 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778698736|ref|XP_011654591.1|1.1e-71100.00PREDICTED: cytochrome b5 isoform E [Cucumis sativus][more]
gi|659074510|ref|XP_008437642.1|1.0e-6997.01PREDICTED: cytochrome b5 isoform A [Cucumis melo][more]
gi|399920236|gb|AFP55583.1|6.3e-6488.06yellow stripe-like protein [Rosa rugosa][more]
gi|747075053|ref|XP_011084546.1|8.2e-6488.06PREDICTED: cytochrome b5 isoform E-like [Sesamum indicum][more]
gi|596010715|ref|XP_007218582.1|1.1e-6387.31hypothetical protein PRUPE_ppa013232mg [Prunus persica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001199Cyt_B5-like_heme/steroid-bd
IPR018506Cyt_B5_heme-BS
Vocabulary: Molecular Function
TermDefinition
GO:0020037heme binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006040 amino sugar metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0019745 pentacyclic triterpenoid biosynthetic process
biological_process GO:0016126 sterol biosynthetic process
biological_process GO:0008150 biological_process
cellular_component GO:0009535 chloroplast thylakoid membrane
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005774 vacuolar membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0004128 cytochrome-b5 reductase activity, acting on NAD(P)H
molecular_function GO:0020037 heme binding
molecular_function GO:0046872 metal ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.202340.1Cucsa.202340.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001199Cytochrome b5-like heme/steroid binding domainPRINTSPR00363CYTOCHROMEB5coord: 30..40
score: 3.4E-16coord: 40..54
score: 3.4E-16coord: 55..62
score: 3.4E-16coord: 68..80
score: 3.4
IPR001199Cytochrome b5-like heme/steroid binding domainGENE3DG3DSA:3.10.120.10coord: 5..86
score: 4.3
IPR001199Cytochrome b5-like heme/steroid binding domainPFAMPF00173Cyt-b5coord: 11..80
score: 2.8
IPR001199Cytochrome b5-like heme/steroid binding domainSMARTSM01117Cyt_b5_2coord: 8..81
score: 1.1
IPR001199Cytochrome b5-like heme/steroid binding domainPROFILEPS50255CYTOCHROME_B5_2coord: 5..81
score: 29
IPR001199Cytochrome b5-like heme/steroid binding domainunknownSSF55856Cytochrome b5-like heme/steroid binding domaincoord: 4..87
score: 4.19
IPR018506Cytochrome b5, heme-binding sitePROSITEPS00191CYTOCHROME_B5_1coord: 36..43
scor
NoneNo IPR availablePANTHERPTHR19359CYTOCHROME B5coord: 1..134
score: 1.4E
NoneNo IPR availablePANTHERPTHR19359:SF22CYTOCHROME B5 ISOFORM Acoord: 1..134
score: 1.4E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.202340Cla004873Watermelon (97103) v1cgywmB400
Cucsa.202340Cla021123Watermelon (97103) v1cgywmB398
Cucsa.202340Csa5G139160Cucumber (Chinese Long) v2cgycuB309
Cucsa.202340Csa3G119260Cucumber (Chinese Long) v2cgycuB307
Cucsa.202340MELO3C005907Melon (DHL92) v3.5.1cgymeB367
Cucsa.202340MELO3C005994Melon (DHL92) v3.5.1cgymeB369
Cucsa.202340ClCG01G000210Watermelon (Charleston Gray)cgywcgB375
Cucsa.202340ClCG05G000550Watermelon (Charleston Gray)cgywcgB377
Cucsa.202340CSPI03G07620Wild cucumber (PI 183967)cgycpiB320
Cucsa.202340CmaCh15G010100Cucurbita maxima (Rimu)cgycmaB0599
Cucsa.202340CmaCh02G011390Cucurbita maxima (Rimu)cgycmaB0602
Cucsa.202340CmoCh15G010730Cucurbita moschata (Rifu)cgycmoB0600
Cucsa.202340CmoCh02G011700Cucurbita moschata (Rifu)cgycmoB0603
Cucsa.202340Lsi09G000030Bottle gourd (USVL1VR-Ls)cgylsiB347
Cucsa.202340Lsi05G021600Bottle gourd (USVL1VR-Ls)cgylsiB349
Cucsa.202340Cp4.1LG13g03230Cucurbita pepo (Zucchini)cgycpeB0571
Cucsa.202340Cp4.1LG05g05960Cucurbita pepo (Zucchini)cgycpeB0576
Cucsa.202340MELO3C005994.2Melon (DHL92) v3.6.1cgymedB366
Cucsa.202340MELO3C005907.2Melon (DHL92) v3.6.1cgymedB363
Cucsa.202340CsaV3_3G007770Cucumber (Chinese Long) v3cgycucB325
Cucsa.202340CsaV3_5G000250Cucumber (Chinese Long) v3cgycucB327
Cucsa.202340Cla97C01G000230Watermelon (97103) v2cgywmbB379
Cucsa.202340Bhi12G000587Wax gourdcgywgoB424
Cucsa.202340Carg13998Silver-seed gourdcarcgyB0583
Cucsa.202340Carg26776Silver-seed gourdcarcgyB0651
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cucsa.202340Cucsa.259540Cucumber (Gy14) v1cgycgyB116
The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.202340Cucumber (Gy14) v1cgycgyB000
Cucsa.202340Cucurbita maxima (Rimu)cgycmaB0600
Cucsa.202340Cucurbita maxima (Rimu)cgycmaB0601
Cucsa.202340Cucurbita moschata (Rifu)cgycmoB0597
Cucsa.202340Cucurbita moschata (Rifu)cgycmoB0601
Cucsa.202340Cucurbita moschata (Rifu)cgycmoB0602
Cucsa.202340Wild cucumber (PI 183967)cgycpiB321
Cucsa.202340Wild cucumber (PI 183967)cgycpiB322
Cucsa.202340Cucumber (Chinese Long) v2cgycuB308
Cucsa.202340Melon (DHL92) v3.5.1cgymeB368
Cucsa.202340Watermelon (Charleston Gray)cgywcgB376
Cucsa.202340Watermelon (97103) v1cgywmB397
Cucsa.202340Cucurbita pepo (Zucchini)cgycpeB0570
Cucsa.202340Cucurbita pepo (Zucchini)cgycpeB0573
Cucsa.202340Cucurbita pepo (Zucchini)cgycpeB0574
Cucsa.202340Bottle gourd (USVL1VR-Ls)cgylsiB348
Cucsa.202340Melon (DHL92) v3.6.1cgymedB365
Cucsa.202340Silver-seed gourdcarcgyB1107
Cucsa.202340Silver-seed gourdcarcgyB0617
Cucsa.202340Cucumber (Chinese Long) v3cgycucB326
Cucsa.202340Watermelon (97103) v2cgywmbB380
Cucsa.202340Watermelon (97103) v2cgywmbB381
Cucsa.202340Wax gourdcgywgoB428