Carg26776 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg26776
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	cytochrome b5
Location	Cucurbita_argyrosperma_scaffold_327 : 75683 .. 78109 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTTCACCTTTTGACTTCGTAATTTACTTCTTGTAATTCGAGGGGATTTTTGCCGCACTTTCCGTTGTTCTTTCATTGTTATTATAAAATGATTTTTGATGCCGAATCAGGAACTAATCTGCACTCGGGCTTTGCCGCAAACAACAGGGATCCGAACAATTCGTTGCCTCGTCAACCCTTTTCCCTCTGTAAGTGTGCTTGTTTCTTAGCGCCGACCCACTTCGGCTTCTTATCTAGACTTGGTTTCTGTATTTTCGTTTCGTTTTTTTATATGAAGTTTCAAGTTCTTGGCGTCTGCTCTTTGTAGGGTTTGTGAATCTCGATTGTTTCTTGATTGTTGTAGTGTTAAATGGCTTCGGATCCGAAGGTTCATCTGTTCGAAGAAGTGGCAAAGCACAACAAAACCAAAGATTGTTGGCTTGTAATCTCTGGGAAGGTTGGATTGGATCTCTCTTAGTTGATTGTTCGTATCATTTTTATTTAGCACAGATTATCAGTTCTGCTATAATTTCTGGGCATTTCTCTTTTGATTGGTAACAAAAGCATATGGATATGGCTAATGTTCTTAACTCTTGCTAACTCAGTGTGCCCATTGTATATCTGCTACAAGAGTCTGATATAGATGCCTTAGCTTTGGGGTTTCATTGTTTTGTATGCACTTTCACACATTTCTGTTTCAGATTCTCAAATTCAAATATCTTCTCCCGTTTTCTTCATTGCTTATGTTCATACGCGAGATTCCATATATGCTTTTAATATAGAAGATAATCTAGTCTCATTTTTTTCTGTTCTGTTTCTATTATCTGGTTTTGTTTGTATGACTGAATTACATGTTTATGTAGTGTCTTAATGTTTCTGTGCCATAGTCATCTCAAATGGGGAAATAATGGTGGAGAGCTGTTAGGATGTTTATATTATAGTGTGTGAAGGGCTATGTAAAGGACTGAAATTGACCGAATGATTTATTTGTGAGATTCCTCGTTGGTTGGTGAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACCTCTACCTAATAGATGCGCTTTGAAATCGCGAGGCTGATGGCGATACGTAATGAGACGAAGTGGACAATATCAGTTCGGTGGACTTAGGCTGTTACAAATGGTATCAAAGTCAGACACCGAGTGGTGTGCCAGCGAAGACGTTGGGCTCTCAAGGGGGTGGATTGTGAGATCCCACGTTGGTTAGAGAGGAGAACGAAAGATTTCTTATAAAAGTGTGGAAACCTCTCCCTAACAGACGCGTGTTAAAATCGTGAGGTTGACAGTGATCCATAATGAGCCAAAGCAGACAATATGAGTCAGTGGTGGGCTTGAGATATTACAAATGGTATCAAAGCCAGACATCGAGTGGTGTGCTAGCGAGGACGCTGGGCCTCCAAGGGGGGTGAAATTTGAGATCCCACATTGGTTGGAGAGGAGGACGAAACATTCCTTAAAAGGGTGTGAAAGCCTCTCCCTAACAGACACATTTTAAAATCGTGAGGCCAAAATAGACAATATCAGTTAGCGAGTTAGCGGTGGGCAGATAAGATATCTTTTCTGATGTTTGATTGCCTGTGGCCTTGGCTTTAGGTTTATGATGTAACTCCTTTCATGGAGGATCATCCTGGAGGCGATGAAGTATTGTTAGCAGCAACTGGTAATCCTCTGAGTTCCCTTACAACAAACACTCGATTTTGTTTCCTTGAGTTCTCGTGCAAATGACGATTGCGATGATACGATAACAGTTACTGATTCGAATGTTTTGCAGGAAAAGATGCTACGAACGATTTCGAAGATGTTGGGCACAGCGACTCGGCAAGAGAAATGATGGACAAATATTACATCGGAGAAATCGATCCATCAACAGTTCCTTTGAGGAAGATTTACATTCCATCACAACAAACACAGTACAATCCAGATAAGACAGCTGAGTTTGTGATTAAAATCTTGCAGATTTTGGTTCCCATATTTATTTTGGGTTTGGCATTTGCTGTTCGTCACTACACCAAGAATGAATGAATAAGTGAATGAATAAAGCCTCTAGAGGGTAAACTGCTTATTCATAATGATTATGTTTGGTTTGTGTTTTCATGTGGAGGGTTGGTCGTAGACTAGAGTTGTCTGGATCATATCTTATATTCTACTTAATAATAAGTTAGCTAAGTGCTATGTTCTTGTTTTCTTTTTCAATTTTGCATCTTGAAGTTGTATCAAAATGCGGATTACTATTTGTAACCTTAAAACGATAAGGAATCACTTCGAGGGAAGGTGAGATTTTGATTACCTTGTTGTTCGAATATCTCAACACAAGAACACTTGTTTGAGATTCGAATCATTCCACGAGCAAGATTGATCATGTCAAGTTTGAATGATTCTAAACATGCAACTTAAACGATATAGAATTGTAAAAAAACTTAG

mRNA sequence

CTTTTCACCTTTTGACTTCGTAATTTACTTCTTGTAATTCGAGGGGATTTTTGCCGCACTTTCCGTTGTTCTTTCATTGTTATTATAAAATGATTTTTGATGCCGAATCAGGAACTAATCTGCACTCGGGCTTTGCCGCAAACAACAGGGATCCGAACAATTCGTTGCCTCGTCAACCCTTTTCCCTCTTGTTAAATGGCTTCGGATCCGAAGGTTCATCTGTTCGAAGAAGTGGCAAAGCACAACAAAACCAAAGATTGTTGGCTTGTAATCTCTGGGAAGGTTTATGATGTAACTCCTTTCATGGAGGATCATCCTGGAGGCGATGAAGTATTGTTAGCAGCAACTGGAAAAGATGCTACGAACGATTTCGAAGATGTTGGGCACAGCGACTCGGCAAGAGAAATGATGGACAAATATTACATCGGAGAAATCGATCCATCAACAGTTCCTTTGAGGAAGATTTACATTCCATCACAACAAACACAGTACAATCCAGATAAGACAGCTGAGTTTGTGATTAAAATCTTGCAGATTTTGGTTCCCATATTTATTTTGGGTTTGGCATTTGCTGTTCGTCACTACACCAAGAATGAATGAATAAGTGAATGAATAAAGCCTCTAGAGGGTAAACTGCTTATTCATAATGATTATGTTTGGTTTGTGTTTTCATGTGGAGGGTTGGTCGTAGACTAGAGTTGTCTGGATCATATCTTATATTCTACTTAATAATAAGTTAGCTAAGTGCTATGTTCTTGTTTTCTTTTTCAATTTTGCATCTTGAAGTTGTATCAAAATGCGGATTACTATTTGTAACCTTAAAACGATAAGGAATCACTTCGAGGGAAGGTGAGATTTTGATTACCTTGTTGTTCGAATATCTCAACACAAGAACACTTGTTTGAGATTCGAATCATTCCACGAGCAAGATTGATCATGTCAAGTTTGAATGATTCTAAACATGCAACTTAAACGATATAGAATTGTAAAAAAACTTAG

Coding sequence (CDS)

ATGGCTTCGGATCCGAAGGTTCATCTGTTCGAAGAAGTGGCAAAGCACAACAAAACCAAAGATTGTTGGCTTGTAATCTCTGGGAAGGTTTATGATGTAACTCCTTTCATGGAGGATCATCCTGGAGGCGATGAAGTATTGTTAGCAGCAACTGGAAAAGATGCTACGAACGATTTCGAAGATGTTGGGCACAGCGACTCGGCAAGAGAAATGATGGACAAATATTACATCGGAGAAATCGATCCATCAACAGTTCCTTTGAGGAAGATTTACATTCCATCACAACAAACACAGTACAATCCAGATAAGACAGCTGAGTTTGTGATTAAAATCTTGCAGATTTTGGTTCCCATATTTATTTTGGGTTTGGCATTTGCTGTTCGTCACTACACCAAGAATGAATGA

Protein sequence

MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFEDVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFILGLAFAVRHYTKNE

Homology

[Close]

BLAST of Carg26776 vs. NCBI nr
Match: XP_022922141.1 (cytochrome b5-like isoform X2 [Cucurbita moschata] >XP_022922142.1 cytochrome b5-like isoform X2 [Cucurbita moschata] >XP_023549751.1 cytochrome b5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 279.6 bits (714), Expect = 5.4e-72
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of Carg26776 vs. NCBI nr
Match: XP_022974337.1 (cytochrome b5-like [Cucurbita maxima] >XP_022974338.1 cytochrome b5-like [Cucurbita maxima])

HSP 1 Score: 273.1 bits (697), Expect = 5.1e-70
Identity = 130/134 (97.01%), Postives = 132/134 (98.51%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           MASDPKVH FEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE
Sbjct: 1   MASDPKVHPFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEI+PSTVPLRK+YIP QQTQYNPDKTAEFVIKILQILVPIFI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIEPSTVPLRKVYIPPQQTQYNPDKTAEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of Carg26776 vs. NCBI nr
Match: XP_022137808.1 (cytochrome b5 [Momordica charantia])

HSP 1 Score: 268.9 bits (686), Expect = 9.6e-69
Identity = 127/134 (94.78%), Postives = 132/134 (98.51%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLL+ATGKDATNDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPL+++YIP+QQTQYN DKT EFVIKILQILVPIFI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKRMYIPAQQTQYNSDKTPEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of Carg26776 vs. NCBI nr
Match: XP_022995583.1 (cytochrome b5-like [Cucurbita maxima] >XP_023532432.1 cytochrome b5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 268.5 bits (685), Expect = 1.2e-68
Identity = 128/134 (95.52%), Postives = 131/134 (97.76%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLL+ATGKDA+NDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVP +KIYIPSQQTQYN DKT EFVIKILQILVPIFI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

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BLAST of Carg26776 vs. NCBI nr
Match: XP_011654591.1 (PREDICTED: cytochrome b5 isoform E [Cucumis sativus] >KGN49837.1 hypothetical protein Csa_5G139160 [Cucumis sativus])

HSP 1 Score: 266.5 bits (680), Expect = 4.7e-68
Identity = 126/134 (94.03%), Postives = 131/134 (97.76%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLL+ATGKDATNDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPL+KI+IPSQQ+Q+NPDKT EFVIKILQ LVPI I
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of Carg26776 vs. TAIR10
Match: AT5G53560.1 (cytochrome B5 isoform E)

HSP 1 Score: 221.1 bits (562), Expect = 4.1e-58
Identity = 102/134 (76.12%), Postives = 116/134 (86.57%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           M+SD KV  FEEV+KHNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLL++TGKDATNDFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSD+AR+MMDKY+IGEID S+VP  + Y+  QQ  YN DKT EF+IKILQ LVPI I
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLA  VRHYTK +
Sbjct: 121 LGLALVVRHYTKKD 134

[Close]

BLAST of Carg26776 vs. TAIR10
Match: AT2G32720.1 (cytochrome B5 isoform B)

HSP 1 Score: 191.0 bits (484), Expect = 4.6e-49
Identity = 83/133 (62.41%), Postives = 110/133 (82.71%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           M  + K+    EV++HN+  DCW+VI+GKVY+VT F+EDHPGGD+VLL++TGKDAT+DFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHS+SAREMM++YY+GEIDP+T+P +  Y P +Q  YN DKT+EF+IK+LQ LVP+ I
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAFAVRHYTKN 134
           LGLA  +R YTK+
Sbjct: 121 LGLAVGIRIYTKS 133

[Close]

BLAST of Carg26776 vs. TAIR10
Match: AT5G48810.1 (cytochrome B5 isoform D)

HSP 1 Score: 178.3 bits (451), Expect = 3.1e-45
Identity = 78/136 (57.35%), Postives = 107/136 (78.68%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           M  D KV    EV++H+  KDCW+VI GKVYDVT F++DHPGGDEV+L +TGKDAT+DFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQ--YNPDKTAEFVIKILQILVPI 120
           DVGHS +A+ M+D+YY+G+ID +TVP++  ++P   T+     DK+++FVIK+LQ LVP+
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 121 FILGLAFAVRHYTKNE 135
            ILGLAF +R+YTK +
Sbjct: 121 LILGLAFGIRYYTKTK 136

[Close]

BLAST of Carg26776 vs. TAIR10
Match: AT2G46650.1 (cytochrome B5 isoform C)

HSP 1 Score: 138.7 bits (348), Expect = 2.7e-33
Identity = 66/123 (53.66%), Postives = 88/123 (71.54%), Query Frame = 0


Query: 10  FEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFEDVGHSDSAR 69
           F +VAKH    DCW++I GKVYD++ FM++HPGGD VLLA TGKDA+ DFEDV HS  A+
Sbjct: 7   FHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSKDAK 66

Query: 70  EMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTA--EFVIKILQILVPIFILGLAFAV 129
           E+M KY IG++D STVP+ + YIP  + +    +T   E   K+L  L+P+ ILG+AFA+
Sbjct: 67  ELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILGVAFAL 126

Query: 130 RHY 131
           R Y
Sbjct: 127 RFY 129

[Close]

BLAST of Carg26776 vs. TAIR10
Match: AT1G26340.1 (cytochrome B5 isoform A)

HSP 1 Score: 127.5 bits (319), Expect = 6.2e-30
Identity = 63/127 (49.61%), Postives = 88/127 (69.29%), Query Frame = 0


Query: 6   KVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFEDVGHS 65
           K++  EE A HNK  DCW+VI GKVYDV+ +M++HPGGD+VLLA  GKDAT+DFED GHS
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 66  DSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQI-----LVPIFI 125
             ARE+M+KY+IGE+D S++P     IP  +  Y  D+  + V K+  +     +VP+ I
Sbjct: 66  KDARELMEKYFIGELDESSLP----EIPELKI-YKKDQPQDSVQKLFDLTKQYWVVPVSI 125

Query: 126 LGLAFAV 128
           + ++ AV
Sbjct: 126 ITISVAV 127

[Close]

BLAST of Carg26776 vs. Swiss-Prot
Match: sp|Q42342|CYB5E_ARATH (Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 221.1 bits (562), Expect = 7.5e-57
Identity = 102/134 (76.12%), Postives = 116/134 (86.57%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           M+SD KV  FEEV+KHNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLL++TGKDATNDFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSD+AR+MMDKY+IGEID S+VP  + Y+  QQ  YN DKT EF+IKILQ LVPI I
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLA  VRHYTK +
Sbjct: 121 LGLALVVRHYTKKD 134

[Close]

BLAST of Carg26776 vs. Swiss-Prot
Match: sp|P40934|CYB5_BRAOB (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 5.4e-55
Identity = 101/134 (75.37%), Postives = 114/134 (85.07%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           MAS+ KV  FEEV++HNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLL++TGKDATNDFE
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSD+AR+MM+KYYIGEID STVP  + Y+   Q  YN DKT EF+IKILQ LVPI I
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVPATRTYVAPVQPAYNQDKTPEFMIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLA  VR YTK E
Sbjct: 121 LGLALVVRQYTKKE 134

[Close]

BLAST of Carg26776 vs. Swiss-Prot
Match: sp|P49098|CYB5_TOBAC (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 1.3e-48
Identity = 86/132 (65.15%), Postives = 106/132 (80.30%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           M  + KV    EV++HN  KDCWLVISGKVYDVT F++DHPGGDEVLL+ATGKDAT+DFE
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHS SAR M+D+YY+G+ID +T+P +  Y P  Q  YN DKT+EFV+K+LQ LVP+ I
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEFVVKLLQFLVPLII 120

Query: 121 LGLAFAVRHYTK 133
           LG+AF +R YTK
Sbjct: 121 LGVAFGIRFYTK 132

[Close]

BLAST of Carg26776 vs. Swiss-Prot
Match: sp|P49100|CYB5_ORYSJ (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 193.4 bits (490), Expect = 1.7e-48
Identity = 87/129 (67.44%), Postives = 107/129 (82.95%), Query Frame = 0


Query: 6   KVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFEDVGHS 65
           KV+  EEVAKHN   DCWL+I GKVY+V+ F+EDHPGGD+VLL++TGKDAT+DFEDVGHS
Sbjct: 7   KVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFEDVGHS 66

Query: 66  DSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFILGLAF 125
            +AR MMD+YY+G+ID ST+P R  Y+P +Q  YN DKT EF+IKILQ LVP+ ILGLA 
Sbjct: 67  TTARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKTPEFIIKILQFLVPLAILGLAV 126

Query: 126 AVRHYTKNE 135
           A+R YTK+E
Sbjct: 127 AIRIYTKSE 135

[Close]

BLAST of Carg26776 vs. Swiss-Prot
Match: sp|O48845|CYB5B_ARATH (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 8.3e-48
Identity = 83/133 (62.41%), Postives = 110/133 (82.71%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           M  + K+    EV++HN+  DCW+VI+GKVY+VT F+EDHPGGD+VLL++TGKDAT+DFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHS+SAREMM++YY+GEIDP+T+P +  Y P +Q  YN DKT+EF+IK+LQ LVP+ I
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAFAVRHYTKN 134
           LGLA  +R YTK+
Sbjct: 121 LGLAVGIRIYTKS 133

[Close]

BLAST of Carg26776 vs. TrEMBL
Match: tr|A0A0A0KN46|A0A0A0KN46_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G139160 PE=3 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 3.1e-68
Identity = 126/134 (94.03%), Postives = 131/134 (97.76%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLL+ATGKDATNDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPL+KI+IPSQQ+Q+NPDKT EFVIKILQ LVPI I
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of Carg26776 vs. TrEMBL
Match: tr|A0A1S3AU68|A0A1S3AU68_CUCME (cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103482987 PE=3 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 7.0e-68
Identity = 126/134 (94.03%), Postives = 130/134 (97.01%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           MASDPKV LFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLL+ATGKDATNDFE
Sbjct: 1   MASDPKVRLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPL+KIYIPSQQTQ+NPDKT EFVIKILQ LVP+ I
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQHNPDKTPEFVIKILQFLVPMLI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of Carg26776 vs. TrEMBL
Match: tr|A0A2P6SPD8|A0A2P6SPD8_ROSCH (Putative cytochrome b5-like heme/steroid binding domain-containing protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr1g0382371 PE=3 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 7.2e-65
Identity = 118/134 (88.06%), Postives = 128/134 (95.52%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           MASDPKVH+FEEVAKHN+TKDCWLVISGKVYDVTPFM+DHPGGDEVLL+ATGKDATNDFE
Sbjct: 1   MASDPKVHVFEEVAKHNQTKDCWLVISGKVYDVTPFMDDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSD+AREMMDKYYIGEIDPSTVPL++ YIP  QTQYNPDKT+EFVIKILQ LVP+ I
Sbjct: 61  DVGHSDAAREMMDKYYIGEIDPSTVPLKRTYIPPPQTQYNPDKTSEFVIKILQFLVPLLI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRH+TK E
Sbjct: 121 LGLAFAVRHFTKKE 134

[Close]

BLAST of Carg26776 vs. TrEMBL
Match: tr|J7FWS2|J7FWS2_ROSRU (Yellow stripe-like protein OS=Rosa rugosa OX=74645 PE=4 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 1.6e-64
Identity = 117/134 (87.31%), Postives = 128/134 (95.52%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           MASDPKVH+FEEVAKHN+TKDCWLVISGKVYDVTPFM+DHPGGDEVLL+ATGKDATNDFE
Sbjct: 699 MASDPKVHVFEEVAKHNQTKDCWLVISGKVYDVTPFMDDHPGGDEVLLSATGKDATNDFE 758

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSD+AR+MMDKYYIGEIDPSTVPL++ YIP  QTQYNPDKT+EFVIKILQ LVP+ I
Sbjct: 759 DVGHSDAARDMMDKYYIGEIDPSTVPLKRTYIPPPQTQYNPDKTSEFVIKILQFLVPLLI 818

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRH+TK E
Sbjct: 819 LGLAFAVRHFTKKE 832

[Close]

BLAST of Carg26776 vs. TrEMBL
Match: tr|M5X2R7|M5X2R7_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_2G316100 PE=3 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.0e-63
Identity = 115/134 (85.82%), Postives = 127/134 (94.78%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60
           MASDPKVH+FEEVAKHNKTKDCWLVISGKV+DVTPFM+DHPGGDEVLL+ATGKDATNDFE
Sbjct: 1   MASDPKVHIFEEVAKHNKTKDCWLVISGKVFDVTPFMDDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120
           DVGHSDSAR+MM+KYYIGE+DPSTVPL++IYIP  Q +YNPDK +EFVIKILQ LVP+ I
Sbjct: 61  DVGHSDSARDMMEKYYIGEVDPSTVPLKRIYIPPPQARYNPDKISEFVIKILQFLVPLLI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTK E
Sbjct: 121 LGLAFAVRHYTKKE 134

The following BLAST results are available for this feature:

BLAST of Carg26776 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022922141.1	5.4e-72	100.00	cytochrome b5-like isoform X2 [Cucurbita moschata] >XP_022922142.1 cytochrome b5...	[more]
XP_022974337.1	5.1e-70	97.01	cytochrome b5-like [Cucurbita maxima] >XP_022974338.1 cytochrome b5-like [Cucurb...	[more]
XP_022137808.1	9.6e-69	94.78	cytochrome b5 [Momordica charantia]	[more]
XP_022995583.1	1.2e-68	95.52	cytochrome b5-like [Cucurbita maxima] >XP_023532432.1 cytochrome b5-like [Cucurb...	[more]
XP_011654591.1	4.7e-68	94.03	PREDICTED: cytochrome b5 isoform E [Cucumis sativus] >KGN49837.1 hypothetical pr...	[more]

BLAST of Carg26776 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G53560.1	4.1e-58	76.12	cytochrome B5 isoform E	[more]
AT2G32720.1	4.6e-49	62.41	cytochrome B5 isoform B	[more]
AT5G48810.1	3.1e-45	57.35	cytochrome B5 isoform D	[more]
AT2G46650.1	2.7e-33	53.66	cytochrome B5 isoform C	[more]
AT1G26340.1	6.2e-30	49.61	cytochrome B5 isoform A	[more]

BLAST of Carg26776 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q42342\|CYB5E_ARATH	7.5e-57	76.12	Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2	[more]
sp\|P40934\|CYB5_BRAOB	5.4e-55	75.37	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]
sp\|P49098\|CYB5_TOBAC	1.3e-48	65.15	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
sp\|P49100\|CYB5_ORYSJ	1.7e-48	67.44	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]
sp\|O48845\|CYB5B_ARATH	8.3e-48	62.41	Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1	[more]

BLAST of Carg26776 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KN46\|A0A0A0KN46_CUCSA	3.1e-68	94.03	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G139160 PE=3 SV=1	[more]
tr\|A0A1S3AU68\|A0A1S3AU68_CUCME	7.0e-68	94.03	cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103482987 PE=3 SV=1	[more]
tr\|A0A2P6SPD8\|A0A2P6SPD8_ROSCH	7.2e-65	88.06	Putative cytochrome b5-like heme/steroid binding domain-containing protein OS=Ro...	[more]
tr\|J7FWS2\|J7FWS2_ROSRU	1.6e-64	87.31	Yellow stripe-like protein OS=Rosa rugosa OX=74645 PE=4 SV=1	[more]
tr\|M5X2R7\|M5X2R7_PRUPE	1.0e-63	85.82	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_2G316100 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding

Vocabulary: **INTERPRO**
Term	Definition
IPR018506	Cyt_B5_heme-BS
IPR036400	Cyt_B5-like_heme/steroid_sf
IPR001199	Cyt_B5-like_heme/steroid-bd

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg26776-RA	Carg26776-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 30..40 score: 60.74 coord: 40..54 score: 53.23 coord: 55..62 score: 45.27 coord: 68..80 score: 44.99
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 e-value: 1.5E-30 score: 117.5
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 11..80 e-value: 3.2E-27 score: 94.4
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 29.403
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	G3DSA:3.10.120.10		coord: 2..86 e-value: 1.8E-35 score: 122.6
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	SSF55856	Cytochrome b5-like heme/steroid binding domain	coord: 4..87
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..134
None	No IPR available	PANTHER	PTHR19359:SF22	CYTOCHROME B5 ISOFORM E	coord: 1..134
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg26776	Cla004873	Watermelon (97103) v1	carwmB0574
Carg26776	Csa5G139160	Cucumber (Chinese Long) v2	carcuB0604
Carg26776	MELO3C005907	Melon (DHL92) v3.5.1	carmeB0580
Carg26776	ClCG01G000210	Watermelon (Charleston Gray)	carwcgB0562
Carg26776	Cucsa.202340	Cucumber (Gy14) v1	carcgyB0651
Carg26776	CmaCh02G011390	Cucurbita maxima (Rimu)	carcmaB0808
Carg26776	CmaCh15G010100	Cucurbita maxima (Rimu)	carcmaB0807
Carg26776	CmoCh15G010730	Cucurbita moschata (Rifu)	carcmoB0784
Carg26776	CmoCh02G011700	Cucurbita moschata (Rifu)	carcmoB0785
Carg26776	Lsi09G000030	Bottle gourd (USVL1VR-Ls)	carlsiB524
Carg26776	Cp4.1LG05g05960	Cucurbita pepo (Zucchini)	carcpeB0823
Carg26776	Cp4.1LG13g03230	Cucurbita pepo (Zucchini)	carcpeB0822
Carg26776	CsGy5G000190	Cucumber (Gy14) v2	carcgybB0578
Carg26776	MELO3C005907.2	Melon (DHL92) v3.6.1	carmedB0587
Carg26776	CsaV3_5G000250	Cucumber (Chinese Long) v3	carcucB0611
Carg26776	Cla97C01G000230	Watermelon (97103) v2	carwmbB0602
Carg26776	Bhi12G000587	Wax gourd	carwgoB0688

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg26776	Carg13998	Silver-seed gourd	carcarB491

The following block(s) are covering this gene:


Gene	Organism	Block
Carg26776	Wild cucumber (PI 183967)	carcpiB0610