Cp4.1LG13g03230 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG13g03230
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionCytochrome b5
LocationCp4.1LG13 : 2946237 .. 2948618 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGGCGATAATCAAAGAAAAAGAAAGGAAGAGATTATAATTTTTTCTTTTGATCCAGAGAGACAATCAAATTAGTATACGTCAAGGAAGATAATGTTGTAACAAAACACGATCATCGATCGTTAGGGCAGAAAAGTTTGAATTACATTACCCACTTGTTTTGAAGTTTCCGAGAGATTTTTCCATTGGGAAATCGCCCATGGATTCAAAATCCGCACTACCTTTTCACCTTTTGACTTCGTAATTTACTTCTTGTAATTCCAGGGGATTTTTGCCGCACTTTCCGTTGTTCTTTCATTGTTATTATAAAATGATTTTTGATGCCGAATCAGGAACTAATCTGCACTCGGGCTTTGCCACAAACAACAGGGATCCGAACAATTCGTTGCCTCTTCAACCCTTTTCCCTCTGTAAGTGTGCTTGTTTCTTAGCGCCGACCCACTTCGATTTCTTATCTAGACTTGGTTTCTGTATTTTCGTTTCGTTTTTTGATATGAAGTTTCAAGTTCTTGGCATCTGCTCTTTGTAGGGTTTGTGAATCTCGATTGTTTCTTGATTTTTGTAGTGTTAAATGGCTTCGGATCCGAAGGTTCATCTGTTTGAAGAAGTGGCAAAGCACAACAAAACCAAAGATTGTTGGCTTGTAATCTCTGGGAAGGTTGGATTGGATCTCTCTTAGTTGATTGTTCGTATCATTTTTATTTAGCACAGATTATCAGTTCTGCTATAATTTCTGGATATTTCTCTTTTGATTGGTAACAAAAGCATATGGATATGGCTAATGTTCTTAACTCTTGCTAACTCAGTGTGCCCATTGGATATCTGCTACAAGAGTCTGATATAGATGCCTTAGCTTGGGGATTTCATTGTTTTGTATGCACTTTCACTCATTTCTGTTTCAGATTCTCAAATTCAAATATCCTCACCCGTTTTCTTCATTGCTTAAGTTCATACGCGAGATTCTATATATACTTTTAATGTAGAAGATAATTTAGTCTCATTTCTTTCTGTTCTGTTTCTATTATCTGGATGTTGTTTGTATGACTGAATTACATGTTTATGTAGTGTCTTAATGTTTCTGTGCCATAGAGTCATCTCAAATGGGGAAATAATGGTGGAGAGCTGTTAGGATGTTTATATTATAGTGTGTGAAGGCTATGTAAAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACCTCTACCTAATAGATGCGTTTTGAAATCGCGAGGCTGACGGCGATACGTAATGGGACGAAGTGGACAATATCAGTTAGCGGTGGACTTAGGCTGTTACAAATACTATCAGAGCCAGACATCGAGCGGTGTGCCAGCGAGGATGTTGGGCTCTCAAGTAGGTGGATTGTGAGATCCCACATTGGTTAGAGTGGAGAACGAAAGATTTCTTATAAGGGTGTGGAAACCTCTCCCTAACAGACGCGTTTTAAAATCTTGAGGTTGACAGTGTTCCATAATGAGTCAAAGCGGACAATATGAGTTAGTGGTGGGCTTGGGATATTACAAATGGTATCAAAGGCAGACCCCGAGTGGTGTGCTAGCGAGGACGCTGGGCCCCCAAGGGGGGTGGAATTTGAGATCTCACATTGGTTGGAGAGGAGGACGAAACATTCCTTATAAGGGTGTGGAAACCTCTCCCTAACAGACACGTTTTAAAATCGTGAGGGGTGGGCAGATAAGATATCTTTTCTCATTGTTTGATTGCCTGTGGCCTTGGCTTCAGGTTTATGATGTAACTCCTTTCATGGAGGATCATCCTGGAGGCGATGAAGTATTGTTAGCAGCAACTGATAATCCTCTGAGTTCCCTTATAACAAACACTCGATTTCGTTTCCTTGAGTTCTCGTGCGAATGACGATTGCGACGATATGATAACAGTTACTGATTCGAATGTTTTGCAGGAAAAGATGCTACGAACGATTTCGAAGATGTTGGGCACAGCGACTCGGCAAGAGAAATGATGGACAAATATTACATCGGAGAAATCGATCCATCAACAGTTCCTTTGAGGAAGATTTACATTCCATCACAACAAACACAGTACAATCCAGATAAGACAGCTGAGTTTGTGATTAAAATCTTGCAGATTTTGGTTCCCATATTTATTTTGGGTTTGGCATTTGCTGTTCGTCACTACACCAAGAATGAATGAATAAAGCCTCTAGAGGGTAAACTGCTTATTCATAATGATTATGTTTGGTTTATGTTTTCATGTGGAGGGTTGGTCGTAGACTAGAGTTGTCTGGATCATATCTTATATTCTACTTAATAATAAGTTAGCTAAGTGCTATGTTCTTGTTTTCTTTTTCAATTTTGCATCTTCAAGTTGTATGAAAACGCGGATTATATCTGAAAATTACAGATTT

mRNA sequence

AGGCGATAATCAAAGAAAAAGAAAGGAAGAGATTATAATTTTTTCTTTTGATCCAGAGAGACAATCAAATTAGTATACGTCAAGGAAGATAATGTTGTAACAAAACACGATCATCGATCGTTAGGGCAGAAAAGTTTGAATTACATTACCCACTTGTTTTGAAGTTTCCGAGAGATTTTTCCATTGGGAAATCGCCCATGGATTCAAAATCCGCACTACCTTTTCACCTTTTGACTTCGTAATTTACTTCTTGTAATTCCAGGGGATTTTTGCCGCACTTTCCGTTGTTCTTTCATTGTTATTATAAAATGATTTTTGATGCCGAATCAGGAACTAATCTGCACTCGGGCTTTGCCACAAACAACAGGGATCCGAACAATTCGTTGCCTCTTCAACCCTTTTCCCTCTTGTTAAATGGCTTCGGATCCGAAGGTTCATCTGTTTGAAGAAGTGGCAAAGCACAACAAAACCAAAGATTGTTGGCTTGTAATCTCTGGGAAGGTTTATGATGTAACTCCTTTCATGGAGGATCATCCTGGAGGCGATGAAGTATTGTTAGCAGCAACTGATAATCCTCTGAGAAAAGATGCTACGAACGATTTCGAAGATGTTGGGCACAGCGACTCGGCAAGAGAAATGATGGACAAATATTACATCGGAGAAATCGATCCATCAACAGTTCCTTTGAGGAAGATTTACATTCCATCACAACAAACACAGTACAATCCAGATAAGACAGCTGAGTTTGTGATTAAAATCTTGCAGATTTTGGTTCCCATATTTATTTTGGGTTTGGCATTTGCTGTTCGTCACTACACCAAGAATGAATGAATAAAGCCTCTAGAGGGTAAACTGCTTATTCATAATGATTATGTTTGGTTTATGTTTTCATGTGGAGGGTTGGTCGTAGACTAGAGTTGTCTGGATCATATCTTATATTCTACTTAATAATAAGTTAGCTAAGTGCTATGTTCTTGTTTTCTTTTTCAATTTTGCATCTTCAAGTTGTATGAAAACGCGGATTATATCTGAAAATTACAGATTT

Coding sequence (CDS)

ATGGCTTCGGATCCGAAGGTTCATCTGTTTGAAGAAGTGGCAAAGCACAACAAAACCAAAGATTGTTGGCTTGTAATCTCTGGGAAGGTTTATGATGTAACTCCTTTCATGGAGGATCATCCTGGAGGCGATGAAGTATTGTTAGCAGCAACTGATAATCCTCTGAGAAAAGATGCTACGAACGATTTCGAAGATGTTGGGCACAGCGACTCGGCAAGAGAAATGATGGACAAATATTACATCGGAGAAATCGATCCATCAACAGTTCCTTTGAGGAAGATTTACATTCCATCACAACAAACACAGTACAATCCAGATAAGACAGCTGAGTTTGTGATTAAAATCTTGCAGATTTTGGTTCCCATATTTATTTTGGGTTTGGCATTTGCTGTTCGTCACTACACCAAGAATGAATGA

Protein sequence

MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATDNPLRKDATNDFEDVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFILGLAFAVRHYTKNE
Homology
[Close]
BLAST of Cp4.1LG13g03230 vs. Swiss-Prot
Match: CYB5E_ARATH (Cytochrome b5 isoform E OS=Arabidopsis thaliana GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 212.2 bits (539), Expect = 3.5e-54
Identity = 101/138 (73.19%), Postives = 115/138 (83.33%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATDNPLRKDAT 60
           M+SD KV  FEEV+KHNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLL++T     KDAT
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTG----KDAT 60

Query: 61  NDFEDVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILV 120
           NDFEDVGHSD+AR+MMDKY+IGEID S+VP  + Y+  QQ  YN DKT EF+IKILQ LV
Sbjct: 61  NDFEDVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLV 120

Query: 121 PIFILGLAFAVRHYTKNE 139
           PI ILGLA  VRHYTK +
Sbjct: 121 PILILGLALVVRHYTKKD 134

[Close]
BLAST of Cp4.1LG13g03230 vs. Swiss-Prot
Match: CYB5_BRAOB (Cytochrome b5 OS=Brassica oleracea var. botrytis GN=CYB5 PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 2.5e-52
Identity = 100/138 (72.46%), Postives = 113/138 (81.88%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATDNPLRKDAT 60
           MAS+ KV  FEEV++HNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLL++T     KDAT
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTG----KDAT 60

Query: 61  NDFEDVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILV 120
           NDFEDVGHSD+AR+MM+KYYIGEID STVP  + Y+   Q  YN DKT EF+IKILQ LV
Sbjct: 61  NDFEDVGHSDTARDMMEKYYIGEIDSSTVPATRTYVAPVQPAYNQDKTPEFMIKILQFLV 120

Query: 121 PIFILGLAFAVRHYTKNE 139
           PI ILGLA  VR YTK E
Sbjct: 121 PILILGLALVVRQYTKKE 134

[Close]
BLAST of Cp4.1LG13g03230 vs. Swiss-Prot
Match: CYB5_TOBAC (Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 6.0e-46
Identity = 85/136 (62.50%), Postives = 105/136 (77.21%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATDNPLRKDAT 60
           M  + KV    EV++HN  KDCWLVISGKVYDVT F++DHPGGDEVLL+AT     KDAT
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATG----KDAT 60

Query: 61  NDFEDVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILV 120
           +DFEDVGHS SAR M+D+YY+G+ID +T+P +  Y P  Q  YN DKT+EFV+K+LQ LV
Sbjct: 61  DDFEDVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEFVVKLLQFLV 120

Query: 121 PIFILGLAFAVRHYTK 137
           P+ ILG+AF +R YTK
Sbjct: 121 PLIILGVAFGIRFYTK 132

[Close]
BLAST of Cp4.1LG13g03230 vs. Swiss-Prot
Match: CYB5_ORYSJ (Cytochrome b5 OS=Oryza sativa subsp. japonica GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 184.1 bits (466), Expect = 1.0e-45
Identity = 86/133 (64.66%), Postives = 106/133 (79.70%), Query Frame = 1 

Query: 6   KVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATDNPLRKDATNDFED 65
           KV+  EEVAKHN   DCWL+I GKVY+V+ F+EDHPGGD+VLL++T     KDAT+DFED
Sbjct: 7   KVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTG----KDATDDFED 66

Query: 66  VGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFIL 125
           VGHS +AR MMD+YY+G+ID ST+P R  Y+P +Q  YN DKT EF+IKILQ LVP+ IL
Sbjct: 67  VGHSTTARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKTPEFIIKILQFLVPLAIL 126

Query: 126 GLAFAVRHYTKNE 139
           GLA A+R YTK+E
Sbjct: 127 GLAVAIRIYTKSE 135

[Close]
BLAST of Cp4.1LG13g03230 vs. Swiss-Prot
Match: CYB5B_ARATH (Cytochrome b5 isoform B OS=Arabidopsis thaliana GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 3.9e-45
Identity = 82/137 (59.85%), Postives = 109/137 (79.56%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATDNPLRKDAT 60
           M  + K+    EV++HN+  DCW+VI+GKVY+VT F+EDHPGGD+VLL++T     KDAT
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTG----KDAT 60

Query: 61  NDFEDVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILV 120
           +DFEDVGHS+SAREMM++YY+GEIDP+T+P +  Y P +Q  YN DKT+EF+IK+LQ LV
Sbjct: 61  DDFEDVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLV 120

Query: 121 PIFILGLAFAVRHYTKN 138
           P+ ILGLA  +R YTK+
Sbjct: 121 PLAILGLAVGIRIYTKS 133

[Close]
BLAST of Cp4.1LG13g03230 vs. TrEMBL
Match: A0A0A0KN46_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G139160 PE=3 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 8.1e-66
Identity = 125/138 (90.58%), Postives = 130/138 (94.20%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATDNPLRKDAT 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLL+AT     KDAT
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATG----KDAT 60

Query: 61  NDFEDVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILV 120
           NDFEDVGHSDSAREMMDKYYIGEIDPSTVPL+KI+IPSQQ+Q+NPDKT EFVIKILQ LV
Sbjct: 61  NDFEDVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLV 120

Query: 121 PIFILGLAFAVRHYTKNE 139
           PI ILGLAFAVRHYTKNE
Sbjct: 121 PILILGLAFAVRHYTKNE 134

[Close]
BLAST of Cp4.1LG13g03230 vs. TrEMBL
Match: J7FWS2_ROSRU (Yellow stripe-like protein OS=Rosa rugosa PE=4 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 4.2e-62
Identity = 116/138 (84.06%), Postives = 127/138 (92.03%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATDNPLRKDAT 60
           MASDPKVH+FEEVAKHN+TKDCWLVISGKVYDVTPFM+DHPGGDEVLL+AT     KDAT
Sbjct: 699 MASDPKVHVFEEVAKHNQTKDCWLVISGKVYDVTPFMDDHPGGDEVLLSATG----KDAT 758

Query: 61  NDFEDVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILV 120
           NDFEDVGHSD+AR+MMDKYYIGEIDPSTVPL++ YIP  QTQYNPDKT+EFVIKILQ LV
Sbjct: 759 NDFEDVGHSDAARDMMDKYYIGEIDPSTVPLKRTYIPPPQTQYNPDKTSEFVIKILQFLV 818

Query: 121 PIFILGLAFAVRHYTKNE 139
           P+ ILGLAFAVRH+TK E
Sbjct: 819 PLLILGLAFAVRHFTKKE 832

[Close]
BLAST of Cp4.1LG13g03230 vs. TrEMBL
Match: M5X2R7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013232mg PE=3 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 2.7e-61
Identity = 114/138 (82.61%), Postives = 126/138 (91.30%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATDNPLRKDAT 60
           MASDPKVH+FEEVAKHNKTKDCWLVISGKV+DVTPFM+DHPGGDEVLL+AT     KDAT
Sbjct: 1   MASDPKVHIFEEVAKHNKTKDCWLVISGKVFDVTPFMDDHPGGDEVLLSATG----KDAT 60

Query: 61  NDFEDVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILV 120
           NDFEDVGHSDSAR+MM+KYYIGE+DPSTVPL++IYIP  Q +YNPDK +EFVIKILQ LV
Sbjct: 61  NDFEDVGHSDSARDMMEKYYIGEVDPSTVPLKRIYIPPPQARYNPDKISEFVIKILQFLV 120

Query: 121 PIFILGLAFAVRHYTKNE 139
           P+ ILGLAFAVRHYTK E
Sbjct: 121 PLLILGLAFAVRHYTKKE 134

[Close]
BLAST of Cp4.1LG13g03230 vs. TrEMBL
Match: M1A3J2_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400005465 PE=3 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 1.0e-60
Identity = 115/138 (83.33%), Postives = 124/138 (89.86%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATDNPLRKDAT 60
           M SD KVH+FEEVAKHNKTKDCWL+ISGKVYDVTPFMEDHPGGDEVLL+AT     KDAT
Sbjct: 1   MGSDQKVHVFEEVAKHNKTKDCWLIISGKVYDVTPFMEDHPGGDEVLLSATG----KDAT 60

Query: 61  NDFEDVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILV 120
           NDFEDVGHSDSAREMMDKYYIG+ID STVPL++ YIP QQ  YNPDKT+EFVIKILQILV
Sbjct: 61  NDFEDVGHSDSAREMMDKYYIGDIDQSTVPLKRAYIPPQQAPYNPDKTSEFVIKILQILV 120

Query: 121 PIFILGLAFAVRHYTKNE 139
           P+ ILGLAFAVRHYTK +
Sbjct: 121 PLLILGLAFAVRHYTKEK 134

[Close]
BLAST of Cp4.1LG13g03230 vs. TrEMBL
Match: A0A0V0H1N0_SOLCH (Putative cytochrome b5-like OS=Solanum chacoense PE=3 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 1.0e-60
Identity = 115/138 (83.33%), Postives = 124/138 (89.86%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATDNPLRKDAT 60
           M SD KVH+FEEVAKHNKTKDCWL+ISGKVYDVTPFMEDHPGGDEVLL+AT     KDAT
Sbjct: 1   MGSDQKVHVFEEVAKHNKTKDCWLIISGKVYDVTPFMEDHPGGDEVLLSATG----KDAT 60

Query: 61  NDFEDVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILV 120
           NDFEDVGHSDSAREMMDKYYIG+ID STVPL++ YIP QQ  YNPDKT+EFVIKILQILV
Sbjct: 61  NDFEDVGHSDSAREMMDKYYIGDIDQSTVPLKRAYIPPQQAPYNPDKTSEFVIKILQILV 120

Query: 121 PIFILGLAFAVRHYTKNE 139
           P+ ILGLAFAVRHYTK +
Sbjct: 121 PLLILGLAFAVRHYTKEK 134

[Close]
BLAST of Cp4.1LG13g03230 vs. TAIR10
Match: AT5G53560.1 (AT5G53560.1 cytochrome B5 isoform E)

HSP 1 Score: 212.2 bits (539), Expect = 2.0e-55
Identity = 101/138 (73.19%), Postives = 115/138 (83.33%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATDNPLRKDAT 60
           M+SD KV  FEEV+KHNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLL++T     KDAT
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTG----KDAT 60

Query: 61  NDFEDVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILV 120
           NDFEDVGHSD+AR+MMDKY+IGEID S+VP  + Y+  QQ  YN DKT EF+IKILQ LV
Sbjct: 61  NDFEDVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLV 120

Query: 121 PIFILGLAFAVRHYTKNE 139
           PI ILGLA  VRHYTK +
Sbjct: 121 PILILGLALVVRHYTKKD 134

[Close]
BLAST of Cp4.1LG13g03230 vs. TAIR10
Match: AT2G32720.1 (AT2G32720.1 cytochrome B5 isoform B)

HSP 1 Score: 182.2 bits (461), Expect = 2.2e-46
Identity = 82/137 (59.85%), Postives = 109/137 (79.56%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATDNPLRKDAT 60
           M  + K+    EV++HN+  DCW+VI+GKVY+VT F+EDHPGGD+VLL++T     KDAT
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTG----KDAT 60

Query: 61  NDFEDVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILV 120
           +DFEDVGHS+SAREMM++YY+GEIDP+T+P +  Y P +Q  YN DKT+EF+IK+LQ LV
Sbjct: 61  DDFEDVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLV 120

Query: 121 PIFILGLAFAVRHYTKN 138
           P+ ILGLA  +R YTK+
Sbjct: 121 PLAILGLAVGIRIYTKS 133

[Close]
BLAST of Cp4.1LG13g03230 vs. TAIR10
Match: AT5G48810.1 (AT5G48810.1 cytochrome B5 isoform D)

HSP 1 Score: 169.5 bits (428), Expect = 1.5e-42
Identity = 77/140 (55.00%), Postives = 106/140 (75.71%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATDNPLRKDAT 60
           M  D KV    EV++H+  KDCW+VI GKVYDVT F++DHPGGDEV+L +T     KDAT
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTG----KDAT 60

Query: 61  NDFEDVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQ--YNPDKTAEFVIKILQI 120
           +DFEDVGHS +A+ M+D+YY+G+ID +TVP++  ++P   T+     DK+++FVIK+LQ 
Sbjct: 61  DDFEDVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQF 120

Query: 121 LVPIFILGLAFAVRHYTKNE 139
           LVP+ ILGLAF +R+YTK +
Sbjct: 121 LVPLLILGLAFGIRYYTKTK 136

[Close]
BLAST of Cp4.1LG13g03230 vs. TAIR10
Match: AT2G46650.1 (AT2G46650.1 cytochrome B5 isoform C)

HSP 1 Score: 129.8 bits (325), Expect = 1.3e-30
Identity = 65/127 (51.18%), Postives = 87/127 (68.50%), Query Frame = 1 

Query: 10  FEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATDNPLRKDATNDFEDVGHS 69
           F +VAKH    DCW++I GKVYD++ FM++HPGGD VLLA T     KDA+ DFEDV HS
Sbjct: 7   FHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTG----KDASIDFEDVNHS 66

Query: 70  DSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTA--EFVIKILQILVPIFILGL 129
             A+E+M KY IG++D STVP+ + YIP  + +    +T   E   K+L  L+P+ ILG+
Sbjct: 67  KDAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILGV 126

Query: 130 AFAVRHY 135
           AFA+R Y
Sbjct: 127 AFALRFY 129

[Close]
BLAST of Cp4.1LG13g03230 vs. TAIR10
Match: AT1G26340.1 (AT1G26340.1 cytochrome B5 isoform A)

HSP 1 Score: 118.6 bits (296), Expect = 3.0e-27
Identity = 62/131 (47.33%), Postives = 87/131 (66.41%), Query Frame = 1 

Query: 6   KVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATDNPLRKDATNDFED 65
           K++  EE A HNK  DCW+VI GKVYDV+ +M++HPGGD+VLLA       KDAT+DFED
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAG----KDATDDFED 65

Query: 66  VGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQI-----LV 125
            GHS  ARE+M+KY+IGE+D S++P     IP  +  Y  D+  + V K+  +     +V
Sbjct: 66  AGHSKDARELMEKYFIGELDESSLP----EIPELKI-YKKDQPQDSVQKLFDLTKQYWVV 125

Query: 126 PIFILGLAFAV 132
           P+ I+ ++ AV
Sbjct: 126 PVSIITISVAV 127

[Close]
BLAST of Cp4.1LG13g03230 vs. NCBI nr
Match: gi|778698736|ref|XP_011654591.1| (PREDICTED: cytochrome b5 isoform E [Cucumis sativus])

HSP 1 Score: 257.7 bits (657), Expect = 1.2e-65
Identity = 125/138 (90.58%), Postives = 130/138 (94.20%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATDNPLRKDAT 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLL+AT     KDAT
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATG----KDAT 60

Query: 61  NDFEDVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILV 120
           NDFEDVGHSDSAREMMDKYYIGEIDPSTVPL+KI+IPSQQ+Q+NPDKT EFVIKILQ LV
Sbjct: 61  NDFEDVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLV 120

Query: 121 PIFILGLAFAVRHYTKNE 139
           PI ILGLAFAVRHYTKNE
Sbjct: 121 PILILGLAFAVRHYTKNE 134

[Close]
BLAST of Cp4.1LG13g03230 vs. NCBI nr
Match: gi|659074510|ref|XP_008437642.1| (PREDICTED: cytochrome b5 isoform A [Cucumis melo])

HSP 1 Score: 256.5 bits (654), Expect = 2.6e-65
Identity = 125/138 (90.58%), Postives = 129/138 (93.48%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATDNPLRKDAT 60
           MASDPKV LFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLL+AT     KDAT
Sbjct: 1   MASDPKVRLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATG----KDAT 60

Query: 61  NDFEDVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILV 120
           NDFEDVGHSDSAREMMDKYYIGEIDPSTVPL+KIYIPSQQTQ+NPDKT EFVIKILQ LV
Sbjct: 61  NDFEDVGHSDSAREMMDKYYIGEIDPSTVPLKKIYIPSQQTQHNPDKTPEFVIKILQFLV 120

Query: 121 PIFILGLAFAVRHYTKNE 139
           P+ ILGLAFAVRHYTKNE
Sbjct: 121 PMLILGLAFAVRHYTKNE 134

[Close]
BLAST of Cp4.1LG13g03230 vs. NCBI nr
Match: gi|399920236|gb|AFP55583.1| (yellow stripe-like protein [Rosa rugosa])

HSP 1 Score: 245.4 bits (625), Expect = 6.0e-62
Identity = 116/138 (84.06%), Postives = 127/138 (92.03%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATDNPLRKDAT 60
           MASDPKVH+FEEVAKHN+TKDCWLVISGKVYDVTPFM+DHPGGDEVLL+AT     KDAT
Sbjct: 699 MASDPKVHVFEEVAKHNQTKDCWLVISGKVYDVTPFMDDHPGGDEVLLSATG----KDAT 758

Query: 61  NDFEDVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILV 120
           NDFEDVGHSD+AR+MMDKYYIGEIDPSTVPL++ YIP  QTQYNPDKT+EFVIKILQ LV
Sbjct: 759 NDFEDVGHSDAARDMMDKYYIGEIDPSTVPLKRTYIPPPQTQYNPDKTSEFVIKILQFLV 818

Query: 121 PIFILGLAFAVRHYTKNE 139
           P+ ILGLAFAVRH+TK E
Sbjct: 819 PLLILGLAFAVRHFTKKE 832

[Close]
BLAST of Cp4.1LG13g03230 vs. NCBI nr
Match: gi|596010715|ref|XP_007218582.1| (hypothetical protein PRUPE_ppa013232mg [Prunus persica])

HSP 1 Score: 242.7 bits (618), Expect = 3.9e-61
Identity = 114/138 (82.61%), Postives = 126/138 (91.30%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATDNPLRKDAT 60
           MASDPKVH+FEEVAKHNKTKDCWLVISGKV+DVTPFM+DHPGGDEVLL+AT     KDAT
Sbjct: 1   MASDPKVHIFEEVAKHNKTKDCWLVISGKVFDVTPFMDDHPGGDEVLLSATG----KDAT 60

Query: 61  NDFEDVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILV 120
           NDFEDVGHSDSAR+MM+KYYIGE+DPSTVPL++IYIP  Q +YNPDK +EFVIKILQ LV
Sbjct: 61  NDFEDVGHSDSARDMMEKYYIGEVDPSTVPLKRIYIPPPQARYNPDKISEFVIKILQFLV 120

Query: 121 PIFILGLAFAVRHYTKNE 139
           P+ ILGLAFAVRHYTK E
Sbjct: 121 PLLILGLAFAVRHYTKKE 134

[Close]
BLAST of Cp4.1LG13g03230 vs. NCBI nr
Match: gi|645258004|ref|XP_008234680.1| (PREDICTED: cytochrome b5 isoform A [Prunus mume])

HSP 1 Score: 240.7 bits (613), Expect = 1.5e-60
Identity = 113/138 (81.88%), Postives = 125/138 (90.58%), Query Frame = 1 

Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATDNPLRKDAT 60
           MASDPKVH+FEEVAKHNKTKDCWLVISGKV+DVTPFM+DHPGGDEVLL+AT     KDAT
Sbjct: 1   MASDPKVHIFEEVAKHNKTKDCWLVISGKVFDVTPFMDDHPGGDEVLLSATG----KDAT 60

Query: 61  NDFEDVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILV 120
           NDFEDVGHSDSAR+MM+KYYIGE+DPSTVPL++ YIP  Q +YNPDK +EFVIKILQ LV
Sbjct: 61  NDFEDVGHSDSARDMMEKYYIGEVDPSTVPLKRTYIPPPQARYNPDKISEFVIKILQFLV 120

Query: 121 PIFILGLAFAVRHYTKNE 139
           P+ ILGLAFAVRHYTK E
Sbjct: 121 PLLILGLAFAVRHYTKKE 134

The following BLAST results are available for this feature:
BLAST of Cp4.1LG13g03230 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CYB5E_ARATH3.5e-5473.19Cytochrome b5 isoform E OS=Arabidopsis thaliana GN=CYTB5-E PE=1 SV=2[more]
CYB5_BRAOB2.5e-5272.46Cytochrome b5 OS=Brassica oleracea var. botrytis GN=CYB5 PE=1 SV=1[more]
CYB5_TOBAC6.0e-4662.50Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1[more]
CYB5_ORYSJ1.0e-4564.66Cytochrome b5 OS=Oryza sativa subsp. japonica GN=Os05g0108800 PE=2 SV=2[more]
CYB5B_ARATH3.9e-4559.85Cytochrome b5 isoform B OS=Arabidopsis thaliana GN=CYTB5-B PE=1 SV=1[more]
BLAST of Cp4.1LG13g03230 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KN46_CUCSA8.1e-6690.58Uncharacterized protein OS=Cucumis sativus GN=Csa_5G139160 PE=3 SV=1[more]
J7FWS2_ROSRU4.2e-6284.06Yellow stripe-like protein OS=Rosa rugosa PE=4 SV=1[more]
M5X2R7_PRUPE2.7e-6182.61Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013232mg PE=3 SV=1[more]
M1A3J2_SOLTU1.0e-6083.33Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400005465 PE=3 SV=1[more]
A0A0V0H1N0_SOLCH1.0e-6083.33Putative cytochrome b5-like OS=Solanum chacoense PE=3 SV=1[more]
BLAST of Cp4.1LG13g03230 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G53560.12.0e-5573.19 cytochrome B5 isoform E[more]
AT2G32720.12.2e-4659.85 cytochrome B5 isoform B[more]
AT5G48810.11.5e-4255.00 cytochrome B5 isoform D[more]
AT2G46650.11.3e-3051.18 cytochrome B5 isoform C[more]
AT1G26340.13.0e-2747.33 cytochrome B5 isoform A[more]
BLAST of Cp4.1LG13g03230 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778698736|ref|XP_011654591.1|1.2e-6590.58PREDICTED: cytochrome b5 isoform E [Cucumis sativus][more]
gi|659074510|ref|XP_008437642.1|2.6e-6590.58PREDICTED: cytochrome b5 isoform A [Cucumis melo][more]
gi|399920236|gb|AFP55583.1|6.0e-6284.06yellow stripe-like protein [Rosa rugosa][more]
gi|596010715|ref|XP_007218582.1|3.9e-6182.61hypothetical protein PRUPE_ppa013232mg [Prunus persica][more]
gi|645258004|ref|XP_008234680.1|1.5e-6081.88PREDICTED: cytochrome b5 isoform A [Prunus mume][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0020037heme binding
Vocabulary: INTERPRO
TermDefinition
IPR018506Cyt_B5_heme-BS
IPR001199Cyt_B5-like_heme/steroid-bd
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019745 pentacyclic triterpenoid biosynthetic process
biological_process GO:0016126 sterol biosynthetic process
biological_process GO:0055085 transmembrane transport
biological_process GO:0006040 amino sugar metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0008150 biological_process
cellular_component GO:0009535 chloroplast thylakoid membrane
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005774 vacuolar membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0020037 heme binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004128 cytochrome-b5 reductase activity, acting on NAD(P)H
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG13g03230.1Cp4.1LG13g03230.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001199Cytochrome b5-like heme/steroid binding domainPRINTSPR00363CYTOCHROMEB5coord: 72..84
score: 5.1E-6coord: 30..40
score: 5.
IPR001199Cytochrome b5-like heme/steroid binding domainGENE3DG3DSA:3.10.120.10coord: 4..88
score: 4.9
IPR001199Cytochrome b5-like heme/steroid binding domainPFAMPF00173Cyt-b5coord: 11..84
score: 2.8
IPR001199Cytochrome b5-like heme/steroid binding domainSMARTSM01117Cyt_b5_2coord: 8..85
score: 1.0
IPR001199Cytochrome b5-like heme/steroid binding domainPROFILEPS50255CYTOCHROME_B5_2coord: 5..85
score: 26
IPR001199Cytochrome b5-like heme/steroid binding domainunknownSSF55856Cytochrome b5-like heme/steroid binding domaincoord: 4..91
score: 1.44
IPR018506Cytochrome b5, heme-binding sitePROSITEPS00191CYTOCHROME_B5_1coord: 36..43
scor
NoneNo IPR availablePANTHERPTHR19359CYTOCHROME B5coord: 1..138
score: 8.7E
NoneNo IPR availablePANTHERPTHR19359:SF22CYTOCHROME B5 ISOFORM Acoord: 1..138
score: 8.7E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG13g03230Cucsa.202340Cucumber (Gy14) v1cgycpeB0571
Cp4.1LG13g03230CmaCh15G010100Cucurbita maxima (Rimu)cmacpeB305
Cp4.1LG13g03230CmaCh02G011390Cucurbita maxima (Rimu)cmacpeB602
Cp4.1LG13g03230CmoCh02G011700Cucurbita moschata (Rifu)cmocpeB551
Cp4.1LG13g03230CmoCh15G010730Cucurbita moschata (Rifu)cmocpeB268
Cp4.1LG13g03230Cla004873Watermelon (97103) v1cpewmB187
Cp4.1LG13g03230Csa5G139160Cucumber (Chinese Long) v2cpecuB188
Cp4.1LG13g03230MELO3C005907Melon (DHL92) v3.5.1cpemeB148
Cp4.1LG13g03230ClCG01G000210Watermelon (Charleston Gray)cpewcgB167
Cp4.1LG13g03230Lsi09G000030Bottle gourd (USVL1VR-Ls)cpelsiB134
Cp4.1LG13g03230MELO3C005907.2Melon (DHL92) v3.6.1cpemedB175
Cp4.1LG13g03230CsaV3_5G000250Cucumber (Chinese Long) v3cpecucB0226
Cp4.1LG13g03230Bhi12G000587Wax gourdcpewgoB0221
Cp4.1LG13g03230CsGy5G000190Cucumber (Gy14) v2cgybcpeB610
Cp4.1LG13g03230Carg26776Silver-seed gourdcarcpeB0822
Cp4.1LG13g03230Carg13998Silver-seed gourdcarcpeB0713
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG13g03230Cp4.1LG05g05960Cucurbita pepo (Zucchini)cpecpeB227
The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG13g03230Cucurbita pepo (Zucchini)cpecpeB021
Cp4.1LG13g03230Cucurbita pepo (Zucchini)cpecpeB197
Cp4.1LG13g03230Cucurbita pepo (Zucchini)cpecpeB217
Cp4.1LG13g03230Cucurbita maxima (Rimu)cmacpeB343
Cp4.1LG13g03230Cucurbita maxima (Rimu)cmacpeB423
Cp4.1LG13g03230Cucurbita moschata (Rifu)cmocpeB306
Cp4.1LG13g03230Cucurbita moschata (Rifu)cmocpeB384
Cp4.1LG13g03230Wild cucumber (PI 183967)cpecpiB189
Cp4.1LG13g03230Bottle gourd (USVL1VR-Ls)cpelsiB154
Cp4.1LG13g03230Watermelon (Charleston Gray)cpewcgB173
Cp4.1LG13g03230Watermelon (97103) v1cpewmB179
Cp4.1LG13g03230Melon (DHL92) v3.5.1cpemeB176
Cp4.1LG13g03230Melon (DHL92) v3.6.1cpemedB210
Cp4.1LG13g03230Silver-seed gourdcarcpeB0760
Cp4.1LG13g03230Silver-seed gourdcarcpeB1375
Cp4.1LG13g03230Cucumber (Chinese Long) v3cpecucB0204
Cp4.1LG13g03230Wax gourdcpewgoB0244