Cp4.1LG14g05620 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG14g05620
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
Description21 kDa protein, putative
LocationCp4.1LG14 : 843381 .. 844251 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCACCATAAAAAAAACACGAGCCTCATAGATTCTATAGGCAGAGACATAATGAAATCCTTGCTAATCCTTTTCTTCTTCATTTTAACTGCCACATTCACCACCTCCGCCGTGGCTTCTTCTTCCTCCGCCGTGGCTTCCTCATCCTCCGCCATTCAGCCGGTGAAACCACACATCAGAAAGGCCTGCAGGCCGACCCCATATCCACGCCTTTGCGAGACCGCACTCTCCTTATATTCTTCACAAACCAAAAGAACCCAACAAGAGCTTTGTAGGGCTGCTGTGGTTTCTTCCCTCAAAGCTGCACAAAACGCCACCTCCATAATCTCCAAATTATCTCGCCGGAAGGTGTCGCCCTATGAAGCCGAGGTCATCGGAGATTGCATCGATAATCTCAAAGACTCCGTCTACGAGCTTCGTAGAGCTACCACTGCTATCAAAAGCTTGAATCGTGCTAACGACGTGGAGTTTCAGGTTGTTAATCATAAACTTTCAATTTCATTGAATTATTTTAAATTAGATTCTAATGAATATGTTCTTAACCAGTTGAGTAGTATCAAGACGTGGTTGAGCGCGGGAGAGACCGACATTATGACGTGCACCGATGGGTTGAGCGGGGGCGCGGGGTGGAAGGTGAGATCGACCGTGAAGAAAGAGGTGAAGAAATGTAGTGTGAATGTAATAAGGCAGACTAGTAATGCCTTGTCTCTCATTAGCAACTTGAAGTACAAATGATGAGGTGAATTTTAGAAGAAACATCGTGAGAAAATGTTTGATGAATGAATTATTATGATATATAGTTGCAGCTATTAATCTTGTTTACAACCCTTTTAAAACTATATAATATAATTTCATCGTGGAATTTGAATTATC

mRNA sequence

TCACCATAAAAAAAACACGAGCCTCATAGATTCTATAGGCAGAGACATAATGAAATCCTTGCTAATCCTTTTCTTCTTCATTTTAACTGCCACATTCACCACCTCCGCCGTGGCTTCTTCTTCCTCCGCCGTGGCTTCCTCATCCTCCGCCATTCAGCCGGTGAAACCACACATCAGAAAGGCCTGCAGGCCGACCCCATATCCACGCCTTTGCGAGACCGCACTCTCCTTATATTCTTCACAAACCAAAAGAACCCAACAAGAGCTTTGTAGGGCTGCTGTGGTTTCTTCCCTCAAAGCTGCACAAAACGCCACCTCCATAATCTCCAAATTATCTCGCCGGAAGGTGTCGCCCTATGAAGCCGAGGTCATCGGAGATTGCATCGATAATCTCAAAGACTCCGTCTACGAGCTTCGTAGAGCTACCACTGCTATCAAAAGCTTGAATCGTGCTAACGACGTGGAGTTTCAGTTGAGTAGTATCAAGACGTGGTTGAGCGCGGGAGAGACCGACATTATGACGTGCACCGATGGGTTGAGCGGGGGCGCGGGGTGGAAGGTGAGATCGACCGTGAAGAAAGAGGTGAAGAAATGTAGTGTGAATGTAATAAGGCAGACTAGTAATGCCTTGTCTCTCATTAGCAACTTGAAGTACAAATGATGAGGTGAATTTTAGAAGAAACATCGTGAGAAAATGTTTGATGAATGAATTATTATGATATATAGTTGCAGCTATTAATCTTGTTTACAACCCTTTTAAAACTATATAATATAATTTCATCGTGGAATTTGAATTATC

Coding sequence (CDS)

ATGAAATCCTTGCTAATCCTTTTCTTCTTCATTTTAACTGCCACATTCACCACCTCCGCCGTGGCTTCTTCTTCCTCCGCCGTGGCTTCCTCATCCTCCGCCATTCAGCCGGTGAAACCACACATCAGAAAGGCCTGCAGGCCGACCCCATATCCACGCCTTTGCGAGACCGCACTCTCCTTATATTCTTCACAAACCAAAAGAACCCAACAAGAGCTTTGTAGGGCTGCTGTGGTTTCTTCCCTCAAAGCTGCACAAAACGCCACCTCCATAATCTCCAAATTATCTCGCCGGAAGGTGTCGCCCTATGAAGCCGAGGTCATCGGAGATTGCATCGATAATCTCAAAGACTCCGTCTACGAGCTTCGTAGAGCTACCACTGCTATCAAAAGCTTGAATCGTGCTAACGACGTGGAGTTTCAGTTGAGTAGTATCAAGACGTGGTTGAGCGCGGGAGAGACCGACATTATGACGTGCACCGATGGGTTGAGCGGGGGCGCGGGGTGGAAGGTGAGATCGACCGTGAAGAAAGAGGTGAAGAAATGTAGTGTGAATGTAATAAGGCAGACTAGTAATGCCTTGTCTCTCATTAGCAACTTGAAGTACAAATGA

Protein sequence

MKSLLILFFFILTATFTTSAVASSSSAVASSSSAIQPVKPHIRKACRPTPYPRLCETALSLYSSQTKRTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDSVYELRRATTAIKSLNRANDVEFQLSSIKTWLSAGETDIMTCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKYK
Homology
[Close]
BLAST of Cp4.1LG14g05620 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 3.2e-19
Identity = 63/189 (33.33%), Postives = 108/189 (57.14%), Query Frame = 1 

Query: 19  SAVASSSSAVASSSSAIQPVKPHIRKACRPTPYPRLCETALSLYSSQTKRTQQELCRAAV 78
           S +  S+  V  ++++  P    I+ +C  T YP +CE +LS Y+   +   QEL   A+
Sbjct: 6   STLVFSALLVILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTAL 65

Query: 79  VSSLKAAQNATSIISKLSRRK-VSPYEAEVIGDCIDNLKDSVYELRRATTAIKSLNRA-- 138
             SL   Q A + + +L++ K +   +   I DC++ ++DS+  + R+   +K+L+ A  
Sbjct: 66  QVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKG 125

Query: 139 NDVEFQLSSIKTWLSAGETDIMTCTDGLSG-GAGWKVRSTVKKEVKKCSVNVIRQTSNAL 198
           ND  F++S+++TW+SA  TD  TC DG +G G   K++ +V+ +V    V V R TSNAL
Sbjct: 126 NDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQV----VAVARVTSNAL 185

Query: 199 SLISNLKYK 204
           +L++N   K
Sbjct: 186 ALVNNFAAK 190

[Close]
BLAST of Cp4.1LG14g05620 vs. Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 57.0 bits (136), Expect = 2.8e-07
Identity = 54/200 (27.00%), Postives = 97/200 (48.50%), Query Frame = 1 

Query: 4   LLILFFFILTATFTTSAVASSSSAVASSSSAIQPVKPHIRKACRPTPYPRLCETALSLYS 63
           LL+     + AT T        + ++S+S AI      ++  C  T YP LC +A++   
Sbjct: 30  LLLASIVGIAATTTNQNKNQKITTLSSTSHAI------LKSVCSSTLYPELCFSAVAATG 89

Query: 64  SQTKRTQQELCRAAVVSSLKAAQNATSIISKL--SRRKVSPYEAEVIGDCIDNLKDSVYE 123
            +   +Q+E+  A++  + KA ++    + KL   R+ ++P E   + DC++ + +++ E
Sbjct: 90  GKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDCLETIDETLDE 149

Query: 124 LRRATTAIKSLNRANDVEFQLSSIKTWLSAGETDIMTCTDGLS-GGAGWKVRSTVKKEVK 183
           L  A   +    +   +      +KT +S+  T+  TC DG S   A  KVR    K + 
Sbjct: 150 LHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRKVR----KALL 209

Query: 184 KCSVNVIRQTSNALSLISNL 201
           K  V+V    SNAL++I N+
Sbjct: 210 KGQVHVEHMCSNALAMIKNM 219

[Close]
BLAST of Cp4.1LG14g05620 vs. TrEMBL
Match: A0A0A0L671_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176250 PE=4 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 8.3e-75
Identity = 149/199 (74.87%), Postives = 175/199 (87.94%), Query Frame = 1 

Query: 5   LILFFFILTATFTTSAVASSSSAVASSSSAIQPVKPHIRKACRPTPYPRLCETALSLYSS 64
           L++FFFI+  TFT        SAV+SSSS ++PV+PHIRKAC+PTPYPRLCETALSLY+S
Sbjct: 12  LLIFFFIILTTFTP-------SAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYAS 71

Query: 65  QTKRTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDSVYELRR 124
           QTKR QQELCRAA+VSSLKAAQNATSIISKLSRRK+S YEAEVIGDCIDNLKDSV ELRR
Sbjct: 72  QTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSVDELRR 131

Query: 125 ATTAIKSLNRANDVEFQLSSIKTWLSAGETDIMTCTDGLSGGAGWKVRSTVKKEVKKCSV 184
           A+TAIKSL+R+ DV+FQL+SIKTW+SA +TD++TCTDGLSGG+GWKV   +KK+VK CS+
Sbjct: 132 ASTAIKSLSRSKDVDFQLNSIKTWMSAAQTDVITCTDGLSGGSGWKVSKMLKKDVKNCSI 191

Query: 185 NVIRQTSNALSLISNLKYK 204
           NV+RQ SNAL LI+N  YK
Sbjct: 192 NVVRQISNALFLINNFNYK 203

[Close]
BLAST of Cp4.1LG14g05620 vs. TrEMBL
Match: B9R9Q8_RICCO (21 kDa protein, putative OS=Ricinus communis GN=RCOM_1500020 PE=4 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 8.4e-27
Identity = 81/201 (40.30%), Postives = 126/201 (62.69%), Query Frame = 1 

Query: 3   SLLILFFFILTATFTTSAVASSSSAVASSSSAIQPVKPHIRKACRPTPYPRLCETALSLY 62
           S L L  F++  + T +++A +++  + ++      K +++ AC  T YP++C   LS Y
Sbjct: 14  STLFLITFLVFISNTQNSLAITTTKTSKTT-----YKNYLKTACNSTTYPKICYNTLSPY 73

Query: 63  SSQTKRTQQELCRAAVVSSLKAAQNATSIISKLSRRK-VSPYEAEVIGDCIDNLKDSVYE 122
           +S  +    +LC AA+  +LKAA N ++++  LS++K +S  E  VI DC   ++DSV E
Sbjct: 74  TSTIQTNDLKLCNAALTITLKAASNTSAMVKSLSKQKGLSKGEVAVIKDCQYEIEDSVDE 133

Query: 123 LRRATTAIKSLNRANDVEFQLSSIKTWLSAGETDIMTCTDGLSGGAGWKVRSTVKKEVKK 182
           L+++  A+K+L  + D+EFQ+ +IKTW+SA  TD  TCTDG     G KV S VK ++KK
Sbjct: 134 LKQSLKALKNLKGSADMEFQIDNIKTWISAAITDENTCTDGFE---GMKVSSKVKSKIKK 193

Query: 183 CSVNVIRQTSNALSLISNLKY 203
             VNV R TSNAL+LI+ L Y
Sbjct: 194 SIVNVNRLTSNALALINKLSY 206

[Close]
BLAST of Cp4.1LG14g05620 vs. TrEMBL
Match: M5X0K1_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011478mg PE=4 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 4.2e-26
Identity = 83/201 (41.29%), Postives = 120/201 (59.70%), Query Frame = 1 

Query: 4   LLILFFFILTATFTTSAVASSSSAVASSSSAIQPVKPHIRKACRPTPYPRLCETALSLYS 63
           L IL    +    TT A +SSS          Q  K +++ AC  T YP +C  +LS Y+
Sbjct: 21  LPILLLIFMPNMHTTLATSSSS----------QTYKTYVKTACNTTTYPLICYKSLSSYA 80

Query: 64  SQTKRTQQELCRAAVVSSLKAAQNATSIISKLSRRK-VSPYEAEVIGDCIDNLKDSVYEL 123
           S+ K   Q+LC  A+  +LKAA+NA+S++SKL +   ++P E  V+ DCI+N+KDS+ EL
Sbjct: 81  SKVKSDPQKLCTYALSVTLKAAKNASSVVSKLYKNTGLTPSEKGVVKDCIENIKDSIDEL 140

Query: 124 RRATTAIKSLN-RANDVEFQLSSIKTWLSAGETDIMTCTDGLSGGAGWKVRSTVKKEVKK 183
           +++ +++ +L    +DV+ QL  IKTW+SA  TD  TCTDG     G KV + VK  ++ 
Sbjct: 141 KQSVSSMSNLGVSGSDVQLQLDDIKTWVSAVITDDATCTDGFD---GVKVSAAVKTAIRN 200

Query: 184 CSVNVIRQTSNALSLISNLKY 203
             VN  R  SNALSLI +L Y
Sbjct: 201 SIVNAARLASNALSLIDSLIY 208

[Close]
BLAST of Cp4.1LG14g05620 vs. TrEMBL
Match: A0A059DJ16_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A02882 PE=4 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 2.1e-25
Identity = 82/202 (40.59%), Postives = 127/202 (62.87%), Query Frame = 1 

Query: 4   LLILFFFILTATFTTSAVASSSSAVASSSSAIQPVKPHIRKACRPTPYPRLCETALSLYS 63
           L I    +   +  TSA ASSSS   SSSS        I+ +C  T YP+LC   LS Y+
Sbjct: 9   LAIFLLLVANMSAITSAAASSSS---SSSSRNGKDSNFIKTSCSTTTYPKLCNKYLSSYA 68

Query: 64  SQTKRTQQELCRAAVVSSLKAAQNATSIISKLS-RRKVSPYEAEVIGDCIDNLKDSVYEL 123
           S  +    +LC  A+  SL+AA+N +S+++ LS +R ++  EA ++ DCI+NL D++ EL
Sbjct: 69  SVVRSNPWKLCNCALSVSLQAARNTSSLVTTLSKKRSLTKAEASIVKDCIENLGDTIDEL 128

Query: 124 RRATTAIKSLNRA--NDVEFQLSSIKTWLSAGETDIMTCTDGLSGGAGWKVRSTVKKEVK 183
           +++   + +++ A  +DV FQ++++KTW+SA  TD +TCTDG+   A  KV + VK++++
Sbjct: 129 KQSIKTMANVSSAAKDDVAFQMANVKTWVSAAITDEITCTDGI---AERKVSAGVKRKIQ 188

Query: 184 KCSVNVIRQTSNALSLISNLKY 203
               NV R TSN LSLI++LKY
Sbjct: 189 SYIENVARITSNGLSLINSLKY 204

[Close]
BLAST of Cp4.1LG14g05620 vs. TrEMBL
Match: A0A067JJ47_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_25384 PE=4 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 3.5e-25
Identity = 79/201 (39.30%), Postives = 130/201 (64.68%), Query Frame = 1 

Query: 4   LLILFFFILTAT----FTTSAVASSSSAVASSSSAIQPVKPHIRKACRPTPYPRLCETAL 63
           LL+ F   ++ T     +T+A A++++  A++ ++ +  + +++ AC+ T YP+LC ++L
Sbjct: 12  LLLTFVLFISNTKNTAASTTATATTTTTTAATKTSNKTYRNYLKTACKSTTYPKLCYSSL 71

Query: 64  SLYSSQTKRTQQELCRAAVVSSLKAAQNATSIISKLS-RRKVSPYEAEVIGDCIDNLKDS 123
           S YSS  K     LC  A+  SL+ + N +  ++ LS ++++S  EAEV+ DCID + DS
Sbjct: 72  SSYSSIIKTDDLILCTTALNVSLQISINTSYFMTDLSNQQELSETEAEVVQDCIDEIGDS 131

Query: 124 VYELRRATTAIKSLN-RANDVEFQLSSIKTWLSAGETDIMTCTDGLSGGAGWKVRSTVKK 183
           +YEL ++ + + SL+  ++DV FQ+S+IKTW+SA  TD   CTDG     G KV S VK 
Sbjct: 132 IYELNQSLSDLSSLDFNSSDVRFQISNIKTWVSAAITDDYGCTDGFD---GTKVSSAVKS 191

Query: 184 EVKKCSVNVIRQTSNALSLIS 199
           ++KK  +NV R TSNAL+L++
Sbjct: 192 KIKKAILNVSRITSNALALVN 209

[Close]
BLAST of Cp4.1LG14g05620 vs. TAIR10
Match: AT5G51520.1 (AT5G51520.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 105.5 bits (262), Expect = 3.8e-23
Identity = 71/203 (34.98%), Postives = 111/203 (54.68%), Query Frame = 1 

Query: 10  FILTATFTTSAVASSSSAVASSSSAIQPVKPHIRKACRPTPYPRLCETALSLYSSQTKRT 69
           + LT  F   A  +      S+SS  Q  K  ++ AC  T YP  C  +LS YSS  K  
Sbjct: 6   YSLTIVFVFFASTNIQKTSGSASSYSQNHKTFVKTACNSTTYPDKCYKSLSSYSSNIKSD 65

Query: 70  QQELCRAAVVSSLKAAQNATSIISKL----------SRRKVSPYEAEVIGDCIDNLKDSV 129
             +LC  A+  ++K+A+ ATS++SKL           + K+ P EA ++ DC++ +KD++
Sbjct: 66  PIKLCTTALNLNVKSAKEATSVVSKLLKMSQKSTAGRKGKMLP-EALILKDCLEEMKDTI 125

Query: 130 YELRRATTAIKSLNRANDVEFQLSSIKTWLSAGETDIMTCTDGLSGGAGWKVRSTVKKEV 189
            EL++A T +K+L     +   +++++TW+S+  TD  TCTDG       KV    KK+V
Sbjct: 126 IELKQAITEMKNLQDGGSMAEHITNVRTWVSSALTDEGTCTDGFE---EVKVNKETKKKV 185

Query: 190 KKCSVNVIRQTSNALSLISNLKY 203
            K    +   TSN L+LI+NL+Y
Sbjct: 186 NKVVEELATTTSNTLALITNLRY 204

[Close]
BLAST of Cp4.1LG14g05620 vs. TAIR10
Match: AT4G25250.1 (AT4G25250.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 94.4 bits (233), Expect = 8.9e-20
Identity = 64/198 (32.32%), Postives = 105/198 (53.03%), Query Frame = 1 

Query: 5   LILFFFILTATFTTSAVASSSSAVASSSSAIQPVKPHIRKACRPTPYPRLCETALSLYSS 64
           L L  FI ++ F  +A        A+     Q    +++ AC  T YP +C   LS YSS
Sbjct: 9   LTLMVFINSSNFPKTA--------ATPPGTYQNHTTYVKTACNSTTYPTMCYNCLSSYSS 68

Query: 65  QTKRTQQELCRAAVVSSLKAAQNATSIISKL--SRRKVSPYEAEVIGDCIDNLKDSVYEL 124
             K    +LC  ++  ++K+A+NAT ++S L    +    +E  ++ DC+D +KD++ EL
Sbjct: 69  TIKSDPIKLCTTSLNLNVKSAKNATLVVSNLLQKAKAAKSHEVSILKDCVDEMKDTIDEL 128

Query: 125 RRATTAIKSL-NRANDVEFQLSSIKTWLSAGETDIMTCTDGLSGGAGWKVRSTVKKEVKK 184
           ++A   +K +       E  L ++KTW+S+  TD  TCTDG   G   +V    KK+VKK
Sbjct: 129 KQAVAEMKYVRGGGKTTEEHLKNVKTWVSSALTDEGTCTDGFEEG---RVNVETKKKVKK 188

Query: 185 CSVNVIRQTSNALSLISN 200
               + + TSN L+L+++
Sbjct: 189 AISELSKTTSNTLALLTH 195

[Close]
BLAST of Cp4.1LG14g05620 vs. TAIR10
Match: AT5G62350.1 (AT5G62350.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 90.1 bits (222), Expect = 1.7e-18
Identity = 60/195 (30.77%), Postives = 106/195 (54.36%), Query Frame = 1 

Query: 8   FFFILTATFTTSAVASSSSAVASSSSAIQPVKPHIRKACRPTPYPRLCETALSLYSSQTK 67
           + F+L +     ++  +++  AS + A +     I+ +C+ T YP LC  +LS+Y++  +
Sbjct: 5   YLFVLLSISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYANDIQ 64

Query: 68  RTQQELCRAAVVSSLKAAQNATSIISKLSRRK-VSPYEAEVIGDCIDNLKDSVYELRRAT 127
            + + L   A+  +L  AQ+    +S+L+R K +   E E I DC++ + D+V  L ++ 
Sbjct: 65  TSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRLTKSV 124

Query: 128 TAIKSLNRANDVE---FQLSSIKTWLSAGETDIMTCTDGLSGGAGWKVRSTVKKEVKKCS 187
             +K    A D +   + +S+ +TW SA  TD  TC+DG SG     +   +K  V+   
Sbjct: 125 QELKLCGSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRV---MDGRIKNSVRARI 184

Query: 188 VNVIRQTSNALSLIS 199
           +NV  +TSNALSLI+
Sbjct: 185 MNVGHETSNALSLIN 196

[Close]
BLAST of Cp4.1LG14g05620 vs. TAIR10
Match: AT2G01610.1 (AT2G01610.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 83.2 bits (204), Expect = 2.0e-16
Identity = 58/175 (33.14%), Postives = 95/175 (54.29%), Query Frame = 1 

Query: 42  IRKACRPTPYPRLCETALSLYSSQTKRTQQELCRAAVVSSLKAAQNATSIISKLSRRKVS 101
           IR +C  T YP +C T+LS Y+S  + +   L + A+  SL  A++  + +SKLS R  +
Sbjct: 45  IRTSCNATLYPDVCFTSLSGYASAVQDSPARLAKLAIGVSLSQAKSTAAFLSKLS-RSAA 104

Query: 102 PYEAE-------VIGDCIDNLKDSVYELRRATTAIKSLN----------RANDVEFQLSS 161
            Y  +       VI DC+ N++D+V E+R +   ++ +N                FQ+S+
Sbjct: 105 KYSGDGHQTASAVIRDCVSNVEDAVDEMRGSLRQLRDMNGRGGGTAARRSVETFRFQMSN 164

Query: 162 IKTWLSAGETDIMTCTDGLSG-GAGWKVRSTVKKEVKKCSVNVIRQTSNALSLIS 199
           ++TW+SA  TD  TCTDG      G  +++TV   +++    V R TSNAL+L++
Sbjct: 165 VQTWMSAALTDEDTCTDGFEDMDEGGLIKTTVCDRLEE----VKRLTSNALALVN 214

[Close]
BLAST of Cp4.1LG14g05620 vs. TAIR10
Match: AT1G62770.1 (AT1G62770.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 81.3 bits (199), Expect = 7.8e-16
Identity = 70/206 (33.98%), Postives = 112/206 (54.37%), Query Frame = 1 

Query: 9   FFILTATFTTSAVASSSSAVASSSSAIQPVKPHIRKACRPTPYPRLCETALSLYSSQTKR 68
           F ++  + T   +  SSSA  + S + Q     I  +C+ T YP LC   LS Y+++ + 
Sbjct: 8   FLVILLSIT---ILQSSSATPNRSESDQ----FIVSSCQTTQYPSLCVHTLSAYATKIRH 67

Query: 69  TQ-QELCRAAVVSSLKAAQNATSIISKLSRR----KVSPYEA-----EVIGDCIDNLKDS 128
              Q+L + A++ SL  A++ T  ++KL++     K   Y A     EV+G+ +D L  S
Sbjct: 68  NNDQDLAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQS 127

Query: 129 VYELRRATTAIKSLNRANDVEFQLSSIKTWLSAGETDIMTCTDGLSGGA-GWKVRSTVKK 188
           V EL RA  A+ S     D  +++S+++TW+SA  TD  TC DG S  A G KV+  ++ 
Sbjct: 128 VKELARAGHAVAS----EDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRY 187

Query: 189 EVKKCSVNVIRQTSNALSLISNLKYK 204
           +V    V+V + TSNAL+L++    K
Sbjct: 188 KV----VHVAQVTSNALALVNQFAEK 198

[Close]
BLAST of Cp4.1LG14g05620 vs. NCBI nr
Match: gi|659077238|ref|XP_008439099.1| (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 290.0 bits (741), Expect = 3.1e-75
Identity = 155/201 (77.11%), Postives = 173/201 (86.07%), Query Frame = 1 

Query: 3   SLLILFFFILTATFTTSAVASSSSAVASSSSAIQPVKPHIRKACRPTPYPRLCETALSLY 62
           SL + FFFI+  TFT SAVASSSS V       +PVKPHIRKAC+PTPYPRLCETALSLY
Sbjct: 9   SLTLFFFFIILTTFTPSAVASSSSTV-------RPVKPHIRKACKPTPYPRLCETALSLY 68

Query: 63  SSQTKRTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDSVYEL 122
           +SQTKR QQELCRAA+VSSLKAAQNATSIISKLSRRK+S YE EVIGDCID+LKDSV EL
Sbjct: 69  ASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYETEVIGDCIDDLKDSVDEL 128

Query: 123 RRATTAIKSLNRANDVEFQLSSIKTWLSAGETDIMTCTDGLSGGAGWKVRSTVKKEVKKC 182
           RRA+TAIKSL+R+ DV+FQL+SIKTW SA  TDI+TCTDGLSGG+GWKV   VKKEVK C
Sbjct: 129 RRASTAIKSLSRSKDVDFQLNSIKTWTSAALTDIITCTDGLSGGSGWKVNKKVKKEVKNC 188

Query: 183 SVNVIRQTSNALSLISNLKYK 204
           S+NV+RQ SNALSLI+N  YK
Sbjct: 189 SINVVRQISNALSLINNFNYK 202

[Close]
BLAST of Cp4.1LG14g05620 vs. NCBI nr
Match: gi|449460810|ref|XP_004148137.1| (PREDICTED: 21 kDa protein-like [Cucumis sativus])

HSP 1 Score: 288.1 bits (736), Expect = 1.2e-74
Identity = 149/199 (74.87%), Postives = 175/199 (87.94%), Query Frame = 1 

Query: 5   LILFFFILTATFTTSAVASSSSAVASSSSAIQPVKPHIRKACRPTPYPRLCETALSLYSS 64
           L++FFFI+  TFT        SAV+SSSS ++PV+PHIRKAC+PTPYPRLCETALSLY+S
Sbjct: 12  LLIFFFIILTTFTP-------SAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYAS 71

Query: 65  QTKRTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDSVYELRR 124
           QTKR QQELCRAA+VSSLKAAQNATSIISKLSRRK+S YEAEVIGDCIDNLKDSV ELRR
Sbjct: 72  QTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSVDELRR 131

Query: 125 ATTAIKSLNRANDVEFQLSSIKTWLSAGETDIMTCTDGLSGGAGWKVRSTVKKEVKKCSV 184
           A+TAIKSL+R+ DV+FQL+SIKTW+SA +TD++TCTDGLSGG+GWKV   +KK+VK CS+
Sbjct: 132 ASTAIKSLSRSKDVDFQLNSIKTWMSAAQTDVITCTDGLSGGSGWKVSKMLKKDVKNCSI 191

Query: 185 NVIRQTSNALSLISNLKYK 204
           NV+RQ SNAL LI+N  YK
Sbjct: 192 NVVRQISNALFLINNFNYK 203

[Close]
BLAST of Cp4.1LG14g05620 vs. NCBI nr
Match: gi|470146900|ref|XP_004309057.1| (PREDICTED: 21 kDa protein-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 130.6 bits (327), Expect = 3.2e-27
Identity = 82/197 (41.62%), Postives = 123/197 (62.44%), Query Frame = 1 

Query: 7   LFFFILTATFTTSAVASSSSAVASSSSAIQPVKPHIRKACRPTPYPRLCETALSLYSSQT 66
           LF  +L   F  +  A+SSS+        +  K +++ AC  T YP++C  +LS Y++  
Sbjct: 7   LFALLLFILFINTTTATSSSS--------RTYKTYVKTACNSTTYPQICYKSLSSYATSI 66

Query: 67  KRTQQELCRAAVVSSLKAAQNATSIISKLSRRK-VSPYEAEVIGDCIDNLKDSVYELRRA 126
           K   Q+LC  A+  +LKAA+ + S +SKL+++K ++  EA V+G CI+ +KDSV EL+++
Sbjct: 67  KSNPQKLCSYALSVTLKAAKTSASTVSKLAKKKGITKAEAGVVGACIETIKDSVDELKQS 126

Query: 127 TTAIKSLNRANDVEFQLSSIKTWLSAGETDIMTCTDGLSGGAGWKVRSTVKKEVKKCSVN 186
            TA+K+L  A D+++QL +IKTW+SA  TD  TCTDG       KV + VK  ++K  + 
Sbjct: 127 VTAMKNLGGA-DMQYQLDNIKTWVSASLTDEDTCTDGFD---EQKVSTAVKNTIRKSILT 186

Query: 187 VIRQTSNALSLISNLKY 203
           V R  SNALSLI NL Y
Sbjct: 187 VARLASNALSLIDNLNY 191

[Close]
BLAST of Cp4.1LG14g05620 vs. NCBI nr
Match: gi|255539737|ref|XP_002510933.1| (PREDICTED: 21 kDa protein [Ricinus communis])

HSP 1 Score: 128.6 bits (322), Expect = 1.2e-26
Identity = 81/201 (40.30%), Postives = 126/201 (62.69%), Query Frame = 1 

Query: 3   SLLILFFFILTATFTTSAVASSSSAVASSSSAIQPVKPHIRKACRPTPYPRLCETALSLY 62
           S L L  F++  + T +++A +++  + ++      K +++ AC  T YP++C   LS Y
Sbjct: 14  STLFLITFLVFISNTQNSLAITTTKTSKTT-----YKNYLKTACNSTTYPKICYNTLSPY 73

Query: 63  SSQTKRTQQELCRAAVVSSLKAAQNATSIISKLSRRK-VSPYEAEVIGDCIDNLKDSVYE 122
           +S  +    +LC AA+  +LKAA N ++++  LS++K +S  E  VI DC   ++DSV E
Sbjct: 74  TSTIQTNDLKLCNAALTITLKAASNTSAMVKSLSKQKGLSKGEVAVIKDCQYEIEDSVDE 133

Query: 123 LRRATTAIKSLNRANDVEFQLSSIKTWLSAGETDIMTCTDGLSGGAGWKVRSTVKKEVKK 182
           L+++  A+K+L  + D+EFQ+ +IKTW+SA  TD  TCTDG     G KV S VK ++KK
Sbjct: 134 LKQSLKALKNLKGSADMEFQIDNIKTWISAAITDENTCTDGFE---GMKVSSKVKSKIKK 193

Query: 183 CSVNVIRQTSNALSLISNLKY 203
             VNV R TSNAL+LI+ L Y
Sbjct: 194 SIVNVNRLTSNALALINKLSY 206

[Close]
BLAST of Cp4.1LG14g05620 vs. NCBI nr
Match: gi|645257369|ref|XP_008234383.1| (PREDICTED: 21 kDa protein-like [Prunus mume])

HSP 1 Score: 127.1 bits (318), Expect = 3.5e-26
Identity = 84/201 (41.79%), Postives = 119/201 (59.20%), Query Frame = 1 

Query: 4   LLILFFFILTATFTTSAVASSSSAVASSSSAIQPVKPHIRKACRPTPYPRLCETALSLYS 63
           L IL    +    TT A +SSS          Q  K +++ AC  T YP +C  +LS Y+
Sbjct: 21  LPILLLIFMPNMHTTLATSSSS----------QTYKSYVKTACNTTTYPLICYKSLSSYA 80

Query: 64  SQTKRTQQELCRAAVVSSLKAAQNATSIISKLSRRK-VSPYEAEVIGDCIDNLKDSVYEL 123
           S+ K    +LC  A+  +LKAA+NA+S++SKL +   ++P E  V+ DCIDN+KDS+ EL
Sbjct: 81  SKVKSDPHKLCTYALSVTLKAAKNASSVVSKLYKNTGLTPSEKGVVKDCIDNIKDSIDEL 140

Query: 124 RRATTAIKSLN-RANDVEFQLSSIKTWLSAGETDIMTCTDGLSGGAGWKVRSTVKKEVKK 183
           +++ +++ +L    +DV+ QL  IKTW+SA  TD  TCTDG     G KV + VK  +K 
Sbjct: 141 KQSVSSMSNLGVSGSDVQLQLDDIKTWVSAVITDDATCTDGFD---GVKVSTAVKTAIKN 200

Query: 184 CSVNVIRQTSNALSLISNLKY 203
             VN  R  SNALSLI +L Y
Sbjct: 201 SIVNAARLASNALSLIDSLIY 208

The following BLAST results are available for this feature:
BLAST of Cp4.1LG14g05620 vs. Swiss-Prot
Match NameE-valueIdentityDescription
21KD_DAUCA3.2e-1933.3321 kDa protein OS=Daucus carota PE=2 SV=1[more]
PME2_ARATH2.8e-0727.00Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2[more]
BLAST of Cp4.1LG14g05620 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L671_CUCSA8.3e-7574.87Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176250 PE=4 SV=1[more]
B9R9Q8_RICCO8.4e-2740.3021 kDa protein, putative OS=Ricinus communis GN=RCOM_1500020 PE=4 SV=1[more]
M5X0K1_PRUPE4.2e-2641.29Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011478mg PE=4 SV=1[more]
A0A059DJ16_EUCGR2.1e-2540.59Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A02882 PE=4 SV=1[more]
A0A067JJ47_JATCU3.5e-2539.30Uncharacterized protein OS=Jatropha curcas GN=JCGZ_25384 PE=4 SV=1[more]
BLAST of Cp4.1LG14g05620 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G51520.13.8e-2334.98 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT4G25250.18.9e-2032.32 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G62350.11.7e-1830.77 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT2G01610.12.0e-1633.14 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G62770.17.8e-1633.98 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
BLAST of Cp4.1LG14g05620 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659077238|ref|XP_008439099.1|3.1e-7577.11PREDICTED: 21 kDa protein-like [Cucumis melo][more]
gi|449460810|ref|XP_004148137.1|1.2e-7474.87PREDICTED: 21 kDa protein-like [Cucumis sativus][more]
gi|470146900|ref|XP_004309057.1|3.2e-2741.62PREDICTED: 21 kDa protein-like [Fragaria vesca subsp. vesca][more]
gi|255539737|ref|XP_002510933.1|1.2e-2640.30PREDICTED: 21 kDa protein [Ricinus communis][more]
gi|645257369|ref|XP_008234383.1|3.5e-2641.79PREDICTED: 21 kDa protein-like [Prunus mume][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR006501Pectinesterase_inhib_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG14g05620.1Cp4.1LG14g05620.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 41..200
score: 1.9
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 42..196
score: 6.9
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 37..196
score: 2.1
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 1..200
score: 2.8
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 40..200
score: 1.57
NoneNo IPR availablePANTHERPTHR31080FAMILY NOT NAMEDcoord: 1..200
score: 4.2
NoneNo IPR availablePANTHERPTHR31080:SF15SUBFAMILY NOT NAMEDcoord: 1..200
score: 4.2