Carg16733 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg16733
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	21 kDa protein-like
Location	Cucurbita_argyrosperma_scaffold_087 : 183440 .. 184204 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAATCCTTGCTAATCCTCTTCTTCTTCATTTTAACTGCCACATTCACCACCTCCGCCGTGGCTTCTTCTCCCTCCGCCGTGGCTTCTTCTTCCTCCGCCATTCAGCCGGTGAAACCACACATCAAAAAGGCCTGCAGGCCGACGCCATATCCACGCCTTTGCGAGACCGCGCTCTCCTTATATTCTTCACAAACCAAAACAACCCAACAAGAGCTTTGTAGGGCTGCCGTGGTTTCTTCCCTCAAAGCTGCACAAAACGCCACCTCCATAATCTCCAAATTATCTCGCCGGAAGGTGTCGCCCTATGAAGCCGAGGTCATCGGAGATTGCATCGATAATCTCAAAGACTCCATCTACGAGCTTCGTAGAGCTACCACTGCTATCAAAAGCTTGAATCGTGCTAACGACCTGAAGTTTCAGGTTGTTAATCATAAACTTTCAATTTCATCAAATTATTTTAAATTAGATTCTAATGAATATGTTCTTAACCAGTTGAGTAGTATCAAGACGTGGTTGAGCGCGGCAGGGACCGACATTGTGACATGCACCGATGGGTTGAGCGGGGGCGCGGGGTGGAAGGTGAGATCGACGGTGAAGAAAGAGGTGAAGAAATGTAGTGTGAATGTAATAAGGCAGACTAGTAACGCCTTGTCTCTCATTAGCAACTTGAAGTACAAATGATGAGGTGAGTTTTAGAAGAAACATCGTGAGAAGATGTTTGATGAATGAATTATTATGATATATAAATGAATTATTATGATA

mRNA sequence

ATGAAATCCTTGCTAATCCTCTTCTTCTTCATTTTAACTGCCACATTCACCACCTCCGCCGTGGCTTCTTCTCCCTCCGCCGTGGCTTCTTCTTCCTCCGCCATTCAGCCGGTGAAACCACACATCAAAAAGGCCTGCAGGCCGACGCCATATCCACGCCTTTGCGAGACCGCGCTCTCCTTATATTCTTCACAAACCAAAACAACCCAACAAGAGCTTTGTAGGGCTGCCGTGGTTTCTTCCCTCAAAGCTGCACAAAACGCCACCTCCATAATCTCCAAATTATCTCGCCGGAAGGTGTCGCCCTATGAAGCCGAGGTCATCGGAGATTGCATCGATAATCTCAAAGACTCCATCTACGAGCTTCGTAGAGCTACCACTGCTATCAAAAGCTTGAATCGTGCTAACGACCTGAAGTTTCAGTTGAGTAGTATCAAGACGTGGTTGAGCGCGGCAGGGACCGACATTGTGACATGCACCGATGGGTTGAGCGGGGGCGCGGGGTGGAAGGTGAGATCGACGGTGAAGAAAGAGGTGAAGAAATGTAGTGTGAATGTAATAAGGCAGACTAGTAACGCCTTGTCTCTCATTAGCAACTTGAAGTACAAATGATGAGGTGAGTTTTAGAAGAAACATCGTGAGAAGATGTTTGATGAATGAATTATTATGATATATAAATGAATTATTATGATA

Coding sequence (CDS)

ATGAAATCCTTGCTAATCCTCTTCTTCTTCATTTTAACTGCCACATTCACCACCTCCGCCGTGGCTTCTTCTCCCTCCGCCGTGGCTTCTTCTTCCTCCGCCATTCAGCCGGTGAAACCACACATCAAAAAGGCCTGCAGGCCGACGCCATATCCACGCCTTTGCGAGACCGCGCTCTCCTTATATTCTTCACAAACCAAAACAACCCAACAAGAGCTTTGTAGGGCTGCCGTGGTTTCTTCCCTCAAAGCTGCACAAAACGCCACCTCCATAATCTCCAAATTATCTCGCCGGAAGGTGTCGCCCTATGAAGCCGAGGTCATCGGAGATTGCATCGATAATCTCAAAGACTCCATCTACGAGCTTCGTAGAGCTACCACTGCTATCAAAAGCTTGAATCGTGCTAACGACCTGAAGTTTCAGTTGAGTAGTATCAAGACGTGGTTGAGCGCGGCAGGGACCGACATTGTGACATGCACCGATGGGTTGAGCGGGGGCGCGGGGTGGAAGGTGAGATCGACGGTGAAGAAAGAGGTGAAGAAATGTAGTGTGAATGTAATAAGGCAGACTAGTAACGCCTTGTCTCTCATTAGCAACTTGAAGTACAAATGA

Protein sequence

MKSLLILFFFILTATFTTSAVASSPSAVASSSSAIQPVKPHIKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLKDSIYELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTDIVTCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKYK

Homology

[Close]

BLAST of Carg16733 vs. NCBI nr
Match: XP_022922937.1 (pectinesterase inhibitor 4-like [Cucurbita moschata])

HSP 1 Score: 316.6 bits (810), Expect = 6.1e-83
Identity = 163/167 (97.60%), Postives = 167/167 (100.00%), Query Frame = 0


Query: 37  PVKPHIKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLS 96
           PVKPHIKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLS
Sbjct: 37  PVKPHIKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLS 96

Query: 97  RRKVSPYEAEVIGDCIDNLKDSIYELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTDI 156
           RRKVSPYEAEVIGDCIDNLKDS+YELRRATTAIKSLNRAN+++FQLSSIKTWLSAAGTDI
Sbjct: 97  RRKVSPYEAEVIGDCIDNLKDSVYELRRATTAIKSLNRANNVEFQLSSIKTWLSAAGTDI 156

Query: 157 VTCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKYK 204
           VTCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKYK
Sbjct: 157 VTCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKYK 203

[Close]

BLAST of Carg16733 vs. NCBI nr
Match: XP_023553135.1 (21 kDa protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 311.6 bits (797), Expect = 1.9e-81
Identity = 160/168 (95.24%), Postives = 165/168 (98.21%), Query Frame = 0


Query: 36  QPVKPHIKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKL 95
           QPVKPHI+KACRPTPYPRLCETALSLYSSQTK TQQELCRAAVVSSLKAAQNATSIISKL
Sbjct: 36  QPVKPHIRKACRPTPYPRLCETALSLYSSQTKRTQQELCRAAVVSSLKAAQNATSIISKL 95

Query: 96  SRRKVSPYEAEVIGDCIDNLKDSIYELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTD 155
           SRRKVSPYEAEVIGDCIDNLKDS+YELRRATTAIKSLNRAND++FQLSSIKTWLSA  TD
Sbjct: 96  SRRKVSPYEAEVIGDCIDNLKDSVYELRRATTAIKSLNRANDVEFQLSSIKTWLSAGETD 155

Query: 156 IVTCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKYK 204
           I+TCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKYK
Sbjct: 156 IMTCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKYK 203

[Close]

BLAST of Carg16733 vs. NCBI nr
Match: XP_022985512.1 (pectinesterase inhibitor 4-like [Cucurbita maxima])

HSP 1 Score: 303.5 bits (776), Expect = 5.3e-79
Identity = 157/168 (93.45%), Postives = 163/168 (97.02%), Query Frame = 0


Query: 36  QPVKPHIKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKL 95
           QPVKPHI+KACRPTPYPRLCETALSLYSSQTK TQQELCRAAVVSSLKAAQNATSIISKL
Sbjct: 29  QPVKPHIRKACRPTPYPRLCETALSLYSSQTKRTQQELCRAAVVSSLKAAQNATSIISKL 88

Query: 96  SRRKVSPYEAEVIGDCIDNLKDSIYELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTD 155
           S RKVSPYEAEVIGDC DNLKDSIYELRRATTAIKSLNRA+D++FQLSSIKTWLSAA T+
Sbjct: 89  SHRKVSPYEAEVIGDCTDNLKDSIYELRRATTAIKSLNRADDVEFQLSSIKTWLSAAETN 148

Query: 156 IVTCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKYK 204
           I+TCTDGLSGGAGWKVRS VKKEVKKCSVNVIRQTSNALSLISNLKYK
Sbjct: 149 IMTCTDGLSGGAGWKVRSMVKKEVKKCSVNVIRQTSNALSLISNLKYK 196

[Close]

BLAST of Carg16733 vs. NCBI nr
Match: XP_008439099.1 (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 267.3 bits (682), Expect = 4.2e-68
Identity = 134/168 (79.76%), Postives = 152/168 (90.48%), Query Frame = 0


Query: 36  QPVKPHIKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKL 95
           +PVKPHI+KAC+PTPYPRLCETALSLY+SQTK  QQELCRAA+VSSLKAAQNATSIISKL
Sbjct: 35  RPVKPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKL 94

Query: 96  SRRKVSPYEAEVIGDCIDNLKDSIYELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTD 155
           SRRK+S YE EVIGDCID+LKDS+ ELRRA+TAIKSL+R+ D+ FQL+SIKTW SAA TD
Sbjct: 95  SRRKMSAYETEVIGDCIDDLKDSVDELRRASTAIKSLSRSKDVDFQLNSIKTWTSAALTD 154

Query: 156 IVTCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKYK 204
           I+TCTDGLSGG+GWKV   VKKEVK CS+NV+RQ SNALSLI+N  YK
Sbjct: 155 IITCTDGLSGGSGWKVNKKVKKEVKNCSINVVRQISNALSLINNFNYK 202

[Close]

BLAST of Carg16733 vs. NCBI nr
Match: XP_004148137.1 (PREDICTED: 21 kDa protein-like [Cucumis sativus] >KGN57278.1 hypothetical protein Csa_3G176250 [Cucumis sativus])

HSP 1 Score: 265.8 bits (678), Expect = 1.2e-67
Identity = 131/168 (77.98%), Postives = 153/168 (91.07%), Query Frame = 0


Query: 36  QPVKPHIKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKL 95
           +PV+PHI+KAC+PTPYPRLCETALSLY+SQTK  QQELCRAA+VSSLKAAQNATSIISKL
Sbjct: 36  RPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKL 95

Query: 96  SRRKVSPYEAEVIGDCIDNLKDSIYELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTD 155
           SRRK+S YEAEVIGDCIDNLKDS+ ELRRA+TAIKSL+R+ D+ FQL+SIKTW+SAA TD
Sbjct: 96  SRRKMSAYEAEVIGDCIDNLKDSVDELRRASTAIKSLSRSKDVDFQLNSIKTWMSAAQTD 155

Query: 156 IVTCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKYK 204
           ++TCTDGLSGG+GWKV   +KK+VK CS+NV+RQ SNAL LI+N  YK
Sbjct: 156 VITCTDGLSGGSGWKVSKMLKKDVKNCSINVVRQISNALFLINNFNYK 203

[Close]

BLAST of Carg16733 vs. TAIR10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 93.6 bits (231), Expect = 1.5e-19
Identity = 56/161 (34.78%), Postives = 92/161 (57.14%), Query Frame = 0


Query: 42  IKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRK-V 101
           I+ +C+ T YP LC  +LS+Y++  +T+ + L   A+  +L  AQ+    +S+L+R K +
Sbjct: 39  IQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKGL 98

Query: 102 SPYEAEVIGDCIDNLKDSIYELRRATTAIKSLNRAND---LKFQLSSIKTWLSAAGTDIV 161
              E E I DC++ + D++  L ++   +K    A D     + +S+ +TW SAA TD  
Sbjct: 99  KKREVEAIKDCVEEMNDTVDRLTKSVQELKLCGSAKDQDQFAYHMSNAQTWTSAALTDEN 158

Query: 162 TCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLIS 199
           TC+DG SG     +   +K  V+   +NV  +TSNALSLI+
Sbjct: 159 TCSDGFSGRV---MDGRIKNSVRARIMNVGHETSNALSLIN 196

[Close]

BLAST of Carg16733 vs. TAIR10
Match: AT5G51520.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 87.0 bits (214), Expect = 1.4e-17
Identity = 58/174 (33.33%), Postives = 90/174 (51.72%), Query Frame = 0


Query: 39  KPHIKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKL--- 98
           K  +K AC  T YP  C  +LS YSS  K+   +LC  A+  ++K+A+ ATS++SKL   
Sbjct: 35  KTFVKTACNSTTYPDKCYKSLSSYSSNIKSDPIKLCTTALNLNVKSAKEATSVVSKLLKM 94

Query: 99  -------SRRKVSPYEAEVIGDCIDNLKDSIYELRRATTAIKSLNRANDLKFQLSSIKTW 158
                   + K+ P EA ++ DC++ +               +L     +   +++++TW
Sbjct: 95  SQKSTAGRKGKMLP-EALILKDCLEEMXXXXXXXXXXXXXXXNLQDGGSMAEHITNVRTW 154

Query: 159 LSAAGTDIVTCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKY 203
           +S+A TD  TCTDG       KV    KK+V K    +   TSN L+LI+NL+Y
Sbjct: 155 VSSALTDEGTCTDGFE---EVKVNKETKKKVNKVVEELATTTSNTLALITNLRY 204

[Close]

BLAST of Carg16733 vs. TAIR10
Match: AT1G62770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 85.5 bits (210), Expect = 4.1e-17
Identity = 62/173 (35.84%), Postives = 99/173 (57.23%), Query Frame = 0


Query: 42  IKKACRPTPYPRLCETALSLYSSQTK-TTQQELCRAAVVSSLKAAQNATSIISKLSRR-- 101
           I  +C+ T YP LC   LS Y+++ +    Q+L + A++ SL  A++ T  ++KL++   
Sbjct: 34  IVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQTALIISLARAKSVTIFVAKLTKETP 93

Query: 102 --KVSPYEA-----EVIGDCIDNLKDSIYELRRATTAIKSLNRANDLKFQLSSIKTWLSA 161
             K   Y A     EV+G+ +D L  S+ EL RA  A+ S     D  +++S+++TW+SA
Sbjct: 94  KFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAGHAVAS----EDFMWKMSNVQTWVSA 153

Query: 162 AGTDIVTCTDGLSGGA-GWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKYK 204
           A TD  TC DG S  A G KV+  ++ +V    V+V + TSNAL+L++    K
Sbjct: 154 ALTDETTCLDGFSERAMGGKVKRLIRYKV----VHVAQVTSNALALVNQFAEK 198

[Close]

BLAST of Carg16733 vs. TAIR10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 84.3 bits (207), Expect = 9.2e-17
Identity = 53/166 (31.93%), Postives = 92/166 (55.42%), Query Frame = 0


Query: 42  IKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRK-V 101
           IK +C  T YP  C  +LS Y+S+ K   ++L   A+  S+  A++A + +S+++  K +
Sbjct: 38  IKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALAVSIARAKSAKTYVSEMTDYKGI 97

Query: 102 SPYEAEVIGDCIDNLKDSIYELRRATTAIKSLNRAN---DLKFQLSSIKTWLSAAGTDIV 161
           +  + E + DC++ + D++  L  +   +K L   +   D  F LS+++TW SAA TD  
Sbjct: 98  TKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGDSGEDFWFCLSNVRTWTSAALTDET 157

Query: 162 TCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKYK 204
            C DG  G A   +   +K  ++   V+V  +TSNAL+LI++   K
Sbjct: 158 ACMDGFGGKA---MAGELKSLIRTHIVSVAEETSNALALINDFASK 200

[Close]

BLAST of Carg16733 vs. TAIR10
Match: AT2G01610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 83.6 bits (205), Expect = 1.6e-16
Identity = 57/175 (32.57%), Postives = 97/175 (55.43%), Query Frame = 0


Query: 42  IKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRKVS 101
           I+ +C  T YP +C T+LS Y+S  + +   L + A+  SL  A++  + +SKLS R  +
Sbjct: 45  IRTSCNATLYPDVCFTSLSGYASAVQDSPARLAKLAIGVSLSQAKSTAAFLSKLS-RSAA 104

Query: 102 PYEAE-------VIGDCIDNLKDSIYELRRATTAIKSLN----------RANDLKFQLSS 161
            Y  +       VI DC+ N++D++ E+R +   ++ +N               +FQ+S+
Sbjct: 105 KYSGDGHQTASAVIRDCVSNVEDAVDEMRGSLRQLRDMNGRGGGTAARRSVETFRFQMSN 164

Query: 162 IKTWLSAAGTDIVTCTDGLSG-GAGWKVRSTVKKEVKKCSVNVIRQTSNALSLIS 199
           ++TW+SAA TD  TCTDG      G  +++TV   +++    V R TSNAL+L++
Sbjct: 165 VQTWMSAALTDEDTCTDGFEDMDEGGLIKTTVCDRLEE----VKRLTSNALALVN 214

[Close]

BLAST of Carg16733 vs. Swiss-Prot
Match: sp|P17407|21KD_DAUCA (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 2.9e-20
Identity = 62/171 (36.26%), Postives = 100/171 (58.48%), Query Frame = 0


Query: 37  PVKPHIKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLS 96
           P    IK +C  T YP +CE +LS Y+   +   QEL   A+  SL   Q A + + +L+
Sbjct: 24  PANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQVSLTRTQQAQTFMKRLN 83

Query: 97  RRK-VSPYEAEVIGDCIDNLKDSIYELRRATTAIKSLNRA--NDLKFQLSSIKTWLSAAG 156
           + K +   +   I DC++ ++DS+  + R+   +K+L+ A  ND  F++S+++TW+SAA 
Sbjct: 84  KFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGNDFTFRMSNVETWVSAAL 143

Query: 157 TDIVTCTDGLSG-GAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKYK 204
           TD  TC DG +G G   K++ +V+ +V    V V R TSNAL+L++N   K
Sbjct: 144 TDETTCMDGFAGKGMDGKIKESVRAQV----VAVARVTSNALALVNNFAAK 190

[Close]

BLAST of Carg16733 vs. Swiss-Prot
Match: sp|Q9SI72|PMEI9_ARATH (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 7.4e-16
Identity = 62/173 (35.84%), Postives = 99/173 (57.23%), Query Frame = 0


Query: 42  IKKACRPTPYPRLCETALSLYSSQTK-TTQQELCRAAVVSSLKAAQNATSIISKLSRR-- 101
           I  +C+ T YP LC   LS Y+++ +    Q+L + A++ SL  A++ T  ++KL++   
Sbjct: 34  IVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQTALIISLARAKSVTIFVAKLTKETP 93

Query: 102 --KVSPYEA-----EVIGDCIDNLKDSIYELRRATTAIKSLNRANDLKFQLSSIKTWLSA 161
             K   Y A     EV+G+ +D L  S+ EL RA  A+ S     D  +++S+++TW+SA
Sbjct: 94  KFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAGHAVAS----EDFMWKMSNVQTWVSA 153

Query: 162 AGTDIVTCTDGLSGGA-GWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKYK 204
           A TD  TC DG S  A G KV+  ++ +V    V+V + TSNAL+L++    K
Sbjct: 154 ALTDETTCLDGFSERAMGGKVKRLIRYKV----VHVAQVTSNALALVNQFAEK 198

[Close]

BLAST of Carg16733 vs. Swiss-Prot
Match: sp|Q9SB37|PMEI7_ARATH (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.7e-15
Identity = 53/166 (31.93%), Postives = 92/166 (55.42%), Query Frame = 0


Query: 42  IKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRK-V 101
           IK +C  T YP  C  +LS Y+S+ K   ++L   A+  S+  A++A + +S+++  K +
Sbjct: 38  IKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALAVSIARAKSAKTYVSEMTDYKGI 97

Query: 102 SPYEAEVIGDCIDNLKDSIYELRRATTAIKSLNRAN---DLKFQLSSIKTWLSAAGTDIV 161
           +  + E + DC++ + D++  L  +   +K L   +   D  F LS+++TW SAA TD  
Sbjct: 98  TKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGDSGEDFWFCLSNVRTWTSAALTDET 157

Query: 162 TCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKYK 204
            C DG  G A   +   +K  ++   V+V  +TSNAL+LI++   K
Sbjct: 158 ACMDGFGGKA---MAGELKSLIRTHIVSVAEETSNALALINDFASK 200

[Close]

BLAST of Carg16733 vs. Swiss-Prot
Match: sp|Q84WE4|PMEI3_ARATH (Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 8.2e-15
Identity = 57/167 (34.13%), Postives = 86/167 (51.50%), Query Frame = 0


Query: 42  IKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATS----IISKLSR 101
           +  +C    YP LC   LS YS  T T +++L +AA+  SL  AQ+A      +   + +
Sbjct: 34  VHSSCEHASYPSLCVRTLSSYSGPTITNRRDLAQAAIKISLSHAQSAAKKLAVVRDSVGK 93

Query: 102 RKVSPYEAEVIGDCIDNLKDSIYELRRATTAIKSL----NRANDLKFQLSSIKTWLSAAG 161
           +K    E   + DC++ + DS+ EL R    +K L      A + ++Q+S+ +TW SAA 
Sbjct: 94  KK---QEKAALVDCVEMIGDSVDELSRTLGVLKHLRVSGGSAKEFRWQMSNAQTWASAAL 153

Query: 162 TDIVTCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNL 201
           TD  TC DG  G         +K EVK+    V R TSNAL +++ L
Sbjct: 154 TDDDTCLDGFQG----MDDGEIKTEVKQWMTKVARVTSNALYMVNQL 193

[Close]

BLAST of Carg16733 vs. Swiss-Prot
Match: sp|Q9SI74|PMI10_ARATH (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 8.2e-15
Identity = 53/161 (32.92%), Postives = 93/161 (57.76%), Query Frame = 0


Query: 41  HIKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSR-RK 100
           +IK +C  T Y  +C  +LS Y+S  ++  Q+L   A+  +L +A++A+  +  +S    
Sbjct: 147 YIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKFVKNISHGGG 206

Query: 101 VSPYEAEVIGDCIDNLKDSIYELRRATTAIKSLNRANDLKFQ--LSSIKTWLSAAGTDIV 160
           ++  E   + DC++ + DS+  L+ +   + S+N  +  KF+  +S ++TW+SAA T+  
Sbjct: 207 LTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETWVSAALTNDD 266

Query: 161 TCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLIS 199
           TC DG S      V++ VK  V++  V V R TSNAL+LI+
Sbjct: 267 TCMDGFS-----LVKTAVKDLVRRHVVEVARLTSNALALIN 302

[Close]

BLAST of Carg16733 vs. TrEMBL
Match: tr|A0A1S3AXZ9|A0A1S3AXZ9_CUCME (21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103483989 PE=4 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 2.8e-68
Identity = 134/168 (79.76%), Postives = 152/168 (90.48%), Query Frame = 0


Query: 36  QPVKPHIKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKL 95
           +PVKPHI+KAC+PTPYPRLCETALSLY+SQTK  QQELCRAA+VSSLKAAQNATSIISKL
Sbjct: 35  RPVKPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKL 94

Query: 96  SRRKVSPYEAEVIGDCIDNLKDSIYELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTD 155
           SRRK+S YE EVIGDCID+LKDS+ ELRRA+TAIKSL+R+ D+ FQL+SIKTW SAA TD
Sbjct: 95  SRRKMSAYETEVIGDCIDDLKDSVDELRRASTAIKSLSRSKDVDFQLNSIKTWTSAALTD 154

Query: 156 IVTCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKYK 204
           I+TCTDGLSGG+GWKV   VKKEVK CS+NV+RQ SNALSLI+N  YK
Sbjct: 155 IITCTDGLSGGSGWKVNKKVKKEVKNCSINVVRQISNALSLINNFNYK 202

[Close]

BLAST of Carg16733 vs. TrEMBL
Match: tr|A0A0A0L671|A0A0A0L671_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G176250 PE=4 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 8.1e-68
Identity = 131/168 (77.98%), Postives = 153/168 (91.07%), Query Frame = 0


Query: 36  QPVKPHIKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKL 95
           +PV+PHI+KAC+PTPYPRLCETALSLY+SQTK  QQELCRAA+VSSLKAAQNATSIISKL
Sbjct: 36  RPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKL 95

Query: 96  SRRKVSPYEAEVIGDCIDNLKDSIYELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTD 155
           SRRK+S YEAEVIGDCIDNLKDS+ ELRRA+TAIKSL+R+ D+ FQL+SIKTW+SAA TD
Sbjct: 96  SRRKMSAYEAEVIGDCIDNLKDSVDELRRASTAIKSLSRSKDVDFQLNSIKTWMSAAQTD 155

Query: 156 IVTCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKYK 204
           ++TCTDGLSGG+GWKV   +KK+VK CS+NV+RQ SNAL LI+N  YK
Sbjct: 156 VITCTDGLSGGSGWKVSKMLKKDVKNCSINVVRQISNALFLINNFNYK 203

[Close]

BLAST of Carg16733 vs. TrEMBL
Match: tr|B9R9Q8|B9R9Q8_RICCO (21 kDa protein, putative OS=Ricinus communis OX=3988 GN=RCOM_1500020 PE=4 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.7e-28
Identity = 76/165 (46.06%), Postives = 110/165 (66.67%), Query Frame = 0


Query: 39  KPHIKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRR 98
           K ++K AC  T YP++C   LS Y+S  +T   +LC AA+  +LKAA N ++++  LS++
Sbjct: 45  KNYLKTACNSTTYPKICYNTLSPYTSTIQTNDLKLCNAALTITLKAASNTSAMVKSLSKQ 104

Query: 99  K-VSPYEAEVIGDCIDNLKDSIYELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTDIV 158
           K +S  E  VI DC   ++DS+ EL+++  A+K+L  + D++FQ+ +IKTW+SAA TD  
Sbjct: 105 KGLSKGEVAVIKDCQYEIEDSVDELKQSLKALKNLKGSADMEFQIDNIKTWISAAITDEN 164

Query: 159 TCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKY 203
           TCTDG     G KV S VK ++KK  VNV R TSNAL+LI+ L Y
Sbjct: 165 TCTDGFE---GMKVSSKVKSKIKKSIVNVNRLTSNALALINKLSY 206

[Close]

BLAST of Carg16733 vs. TrEMBL
Match: tr|A0A2I4E8X2|A0A2I4E8X2_9ROSI (21 kDa protein-like OS=Juglans regia OX=51240 GN=LOC108987391 PE=4 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 3.7e-28
Identity = 77/162 (47.53%), Postives = 111/162 (68.52%), Query Frame = 0


Query: 42  IKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLSRRK-V 101
           IK  C  T YP++C  +LS ++S+ KT   +LC AA+  +L+AAQN +S +SK+S++  +
Sbjct: 53  IKTTCNSTLYPKVCYKSLSYHASEIKTDPLKLCNAALEVALEAAQNTSSTVSKISKQTGL 112

Query: 102 SPYEAEVIGDCIDNLKDSIYELRRATTAIKSLNRANDLKFQLSSIKTWLSAAGTDIVTCT 161
              E  VI DCIDN+KDSIYELR +  A+  L  +N  +FQ+ ++KTW+SAA TD  TC 
Sbjct: 113 LSTETAVIEDCIDNVKDSIYELRDSQNAMGQLGGSN-AEFQIDNVKTWVSAAITDENTCL 172

Query: 162 DGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKY 203
           DGL      KV +TVKK+++K  +N+ + TSNAL+LI+ LKY
Sbjct: 173 DGLD---EMKVNTTVKKKIRKSILNLCKMTSNALALINTLKY 210

[Close]

BLAST of Carg16733 vs. TrEMBL
Match: tr|A0A059DJ16|A0A059DJ16_EUCGR (Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_A02882 PE=4 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 2.9e-25
Identity = 70/164 (42.68%), Postives = 113/164 (68.90%), Query Frame = 0


Query: 42  IKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLS-RRKV 101
           IK +C  T YP+LC   LS Y+S  ++   +LC  A+  SL+AA+N +S+++ LS +R +
Sbjct: 44  IKTSCSTTTYPKLCNKYLSSYASVVRSNPWKLCNCALSVSLQAARNTSSLVTTLSKKRSL 103

Query: 102 SPYEAEVIGDCIDNLKDSIYELRRATTAIKSLNRA--NDLKFQLSSIKTWLSAAGTDIVT 161
           +  EA ++ DCI+NL D+I EL+++   + +++ A  +D+ FQ++++KTW+SAA TD +T
Sbjct: 104 TKAEASIVKDCIENLGDTIDELKQSIKTMANVSSAAKDDVAFQMANVKTWVSAAITDEIT 163

Query: 162 CTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKY 203
           CTDG+   A  KV + VK++++    NV R TSN LSLI++LKY
Sbjct: 164 CTDGI---AERKVSAGVKRKIQSYIENVARITSNGLSLINSLKY 204

The following BLAST results are available for this feature:

BLAST of Carg16733 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022922937.1	6.1e-83	97.60	pectinesterase inhibitor 4-like [Cucurbita moschata]	[more]
XP_023553135.1	1.9e-81	95.24	21 kDa protein-like [Cucurbita pepo subsp. pepo]	[more]
XP_022985512.1	5.3e-79	93.45	pectinesterase inhibitor 4-like [Cucurbita maxima]	[more]
XP_008439099.1	4.2e-68	79.76	PREDICTED: 21 kDa protein-like [Cucumis melo]	[more]
XP_004148137.1	1.2e-67	77.98	PREDICTED: 21 kDa protein-like [Cucumis sativus] >KGN57278.1 hypothetical protei...	[more]

BLAST of Carg16733 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G62350.1	1.5e-19	34.78	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G51520.1	1.4e-17	33.33	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62770.1	4.1e-17	35.84	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	9.2e-17	31.93	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT2G01610.1	1.6e-16	32.57	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

BLAST of Carg16733 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|P17407\|21KD_DAUCA	2.9e-20	36.26	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
sp\|Q9SI72\|PMEI9_ARATH	7.4e-16	35.84	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]
sp\|Q9SB37\|PMEI7_ARATH	1.7e-15	31.93	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
sp\|Q84WE4\|PMEI3_ARATH	8.2e-15	34.13	Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1	[more]
sp\|Q9SI74\|PMI10_ARATH	8.2e-15	32.92	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]

BLAST of Carg16733 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3AXZ9\|A0A1S3AXZ9_CUCME	2.8e-68	79.76	21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103483989 PE=4 SV=1	[more]
tr\|A0A0A0L671\|A0A0A0L671_CUCSA	8.1e-68	77.98	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G176250 PE=4 SV=1	[more]
tr\|B9R9Q8\|B9R9Q8_RICCO	1.7e-28	46.06	21 kDa protein, putative OS=Ricinus communis OX=3988 GN=RCOM_1500020 PE=4 SV=1	[more]
tr\|A0A2I4E8X2\|A0A2I4E8X2_9ROSI	3.7e-28	47.53	21 kDa protein-like OS=Juglans regia OX=51240 GN=LOC108987391 PE=4 SV=1	[more]
tr\|A0A059DJ16\|A0A059DJ16_EUCGR	2.9e-25	42.68	Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_A02882 PE=4 SV...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR035513	Invertase/methylesterase_inhib
IPR006501	Pectinesterase_inhib_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004857	enzyme inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16733-RA	Carg16733-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 37..196 e-value: 5.6E-36 score: 135.5
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 42..196 e-value: 1.9E-28 score: 99.6
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 1..200 e-value: 1.0E-29 score: 101.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	G3DSA:1.20.140.40		coord: 39..201 e-value: 2.6E-19 score: 71.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 40..200
None	No IPR available	PANTHER	PTHR31080:SF15	INVERTASE	coord: 4..201
None	No IPR available	PANTHER	PTHR31080	FAMILY NOT NAMED	coord: 4..201
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 45..200 e-value: 1.46528E-36 score: 126.014

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg16733	Cla006265	Watermelon (97103) v1	carwmB0874
Carg16733	Csa3G176250	Cucumber (Chinese Long) v2	carcuB0885
Carg16733	MELO3C006820	Melon (DHL92) v3.5.1	carmeB0859
Carg16733	CSPI03G16020	Wild cucumber (PI 183967)	carcpiB0905
Carg16733	Cucsa.251560	Cucumber (Gy14) v1	carcgyB0993
Carg16733	CmaCh04G003310	Cucurbita maxima (Rimu)	carcmaB1209
Carg16733	CmaCh16G001850	Cucurbita maxima (Rimu)	carcmaB1206
Carg16733	CmoCh16G001940	Cucurbita moschata (Rifu)	carcmoB1175
Carg16733	Lsi05G013180	Bottle gourd (USVL1VR-Ls)	carlsiB791
Carg16733	Cp4.1LG01g03680	Cucurbita pepo (Zucchini)	carcpeB1216
Carg16733	Cp4.1LG14g05620	Cucurbita pepo (Zucchini)	carcpeB1215
Carg16733	CsGy3G016100	Cucumber (Gy14) v2	carcgybB0859
Carg16733	MELO3C006820.2	Melon (DHL92) v3.6.1	carmedB0869
Carg16733	CsaV3_3G016220	Cucumber (Chinese Long) v3	carcucB0909

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg16733	Silver-seed gourd	carcarB406
Carg16733	Cucurbita moschata (Rifu)	carcmoB1177
Carg16733	Watermelon (Charleston Gray)	carwcgB0847
Carg16733	Watermelon (97103) v1	carwmB0875
Carg16733	Watermelon (97103) v2	carwmbB0910
Carg16733	Wax gourd	carwgoB1019