Lsi05G013180 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi05G013180
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionPlant invertase/pectin methylesterase inhibitor superfamily protein
Locationchr05 : 20940187 .. 20942571 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGTTAAACTAAACACATGTATTCCATTTTTCATGTTTAAATTTCTATGTTAATGTCTTCATGACAAATTATAAAAACTTATGAAAAGATAACTTCTCATGAACCGATTTCTTCAAATATTAGAAAATGTTAATGTCTATCTTTAAAGCCATGTTTTTATGGGAAATTGACATTATCTTTAAAAAAAAAAAAAAAATCCATTAAGAAACTAAAACCATAAACCACCAACAACCATAACCCACATCATCCTCTTTTCAAAAGTAAGAGGAACAATATAAGTTAGAAAGACATCTTTTCAGACTCGGGAGAACCCAGAAGAGAAGGTTAAATATGCTAAATTGTGGGCAACTTTGTTCTCCACTACAAGAAATAACATATACGATAGCTAACGGAAAAAAAAAAACTATAATAGACCTTTTATAGCCTTTTTCGAAAGTCCTGACAACCTATGTTATTAAAAGTCTGAGACGTTCCACAACATCAATTTCAACATTCACTAGAAGATTTTAGTCATGCTATTGACAAACGATGATAAATTCGAGGTAGTTAAGTCGTTTAAAGATATTTCATGCATCTAAGAATGTAAAATTTCTAGTCACAAATGTGAATACCTTCTCAAATTTTTTCTTTCAAACATGTGGCATGTTTTCCGCACCTATAAATATGGTAAAAATCTCCCTTATCTTCATTATTATAACAAAGACAAGCCTAATAGATTCTTTAGAGAGAGACAGAGGGAGATGGAATCTCAAATCCTAAAATCCTTACAAACCCTTTTCTTCTTTATTATTTTAACTACATTCACCACCTCCGCCGTGGCCTCTTCTTCCTCCTCCACCGTCCGGCCAGTACAACCACACATCAGAAAGGCCTGCAAGCCGACGCCATATCCACGCCTTTGCGAGACTGCACTCTCCTTATACGCTTCACAAACCAAAAGAAACCAGCAAGAGCTCTGCAGGGCGGCCATGGCTTCTTCTCTCAAAGCTGCACAAAACGCCACCTCCATCATCTCCAAGCTCTCCCGTCGGAAGGTATCGCCCTATGAAGCCGAAGTCATCGGAGATTGCATCGATAATCTCAATGACTCTGTCGATGAGCTCCGTCGAGCTGCCGCTGCCATCAAAAGCTTGAGTCGTTCTAACGACGTGGAGTTTCAAGTTGTTAAAAACTACTACTTAATCTCATGGTTATTTAAGTTAAAAAACACTTTTAATCTCAAAACTTTCATTAAAGTAACAGTTTATAATTTAATCTTTATACTCTCAAATTTGTAACAATTTAATCCTCTGAACTTTAATAATTAACGTTTTAGTCCTTTTACTTTACTATTTATAATGATTTAGCCTCTAACGTAAAAAAATTCATCAAAATTAAGTGTTAATTTTTATTACATAATGACTCGAGTCTTTTGTACTAATATTTTTTACCATAGGAACTAAATCACTATAAATTGTAAAGTACATAGACTAAATTGTTATTTATTAAAGTTGAGGGGCTAAATTATTACAAATTTAAAAGTAGAAAAACTAAATTGTTATCAGCTAAAATTTAAAGATCAAATTGTTATTTTCATGAAATTTCAAGGACCAAAAGTATTTTTTAATTTAAAAATTTTACGTATACGTGTGTTGTATGTGAATAAAAATTTGAACTTACAATATTTGAAAAAAAAATCGAGATATGTTAATCGCTAATATTTTCTTTTGATAAATACTCTTTAAAAAATCATGGAAATCAAAATAACTTTATATCTAACCTTTTAAGTAAAGTTAAGAATTCTTGTTGTTTAAGATTATATTCACATGATGTGGTCTAATTCAAATATTACTTTCTAAGTCTAAAGTTTTAATCTAGATTAGGTTAAACTTAATCTTTTATTCATATTCTTAACGCAGTTGAGTAGTATAAAAACATGGGCGAGCGCAGCACTGACCGATGTTATCACGTGCACCGACGGGTTGAGCGGCGGCTCGGGGTGGAAGGTGAGATCGAAGTTGAAGAAAGAAGTCAAGAATTGTAGTGCGAATGTAGTAAGGCAGATTAGTAATGCCTTGTTTCTCATCAACAACTTCAACTACAAATAGTGTGAGCTCTGAGTAGTTTTTGAAAGAAACTTCCATGGCCACACCCATTAAACTCACTTGGAACTTGTTCCAAATGAATTCATTTTCCTTTGTAGTTCCACCTTCCATTAAAAAAAAGTATAAAATATGTTTCTTTTTGTGTGTTTTTTTTTTATCATGTGAATTCTTTCCTTAATTTCTCAATGTCTTTTTGGGTATATACATAATTACAATTTCATCACTTTAAATAGTTATACTTCTAAATTTTACATTTACATTTTTTTAATAAATAATACTTCAATGCATTTCAATCTTCA

mRNA sequence

ATGCACAAGCCTAATAGATTCTTTAGAGAGAGACAGAGGGAGATGGAATCTCAAATCCTAAAATCCTTACAAACCCTTTTCTTCTTTATTATTTTAACTACATTCACCACCTCCGCCGTGGCCTCTTCTTCCTCCTCCACCGTCCGGCCAGTACAACCACACATCAGAAAGGCCTGCAAGCCGACGCCATATCCACGCCTTTGCGAGACTGCACTCTCCTTATACGCTTCACAAACCAAAAGAAACCAGCAAGAGCTCTGCAGGGCGGCCATGGCTTCTTCTCTCAAAGCTGCACAAAACGCCACCTCCATCATCTCCAAGCTCTCCCGTCGGAAGGTATCGCCCTATGAAGCCGAAGTCATCGGAGATTGCATCGATAATCTCAATGACTCTGTCGATGAGCTCCGTCGAGCTGCCGCTGCCATCAAAAGCTTGAGTCTAAAGTTAAGAATTCTTTTGAGTAGTATAAAAACATGGGCGAGCGCAGCACTGACCGATGTTATCACGTGCACCGACGGGTTGAGCGGCGGCTCGGGGTGGAAGGTGAGATCGAAGTTGAAGAAAGAAGTCAAGAATTGTAGTGCGAATGTAGTAAGGCAGATTAGTAATGCCTTGTTTCTCATCAACAACTTCAACTACAAATAGTGTGAGCTCTGAGTAGTTTTTGAAAGAAACTTCCATGGCCACACCCATTAAACTCACTTGGAACTTGTTCCAAATGAATTCATTTTCCTTTGTAGTTCCACCTTCCATTAAAAAAAAGTATAAAATATGTTTCTTTTTGTGTGTTTTTTTTTTATCATGTGAATTCTTTCCTTAATTTCTCAATGTCTTTTTGGGTATATACATAATTACAATTTCATCACTTTAAATAGTTATACTTCTAAATTTTACATTTACATTTTTTTAATAAATAATACTTCAATGCATTTCAATCTTCA

Coding sequence (CDS)

ATGCACAAGCCTAATAGATTCTTTAGAGAGAGACAGAGGGAGATGGAATCTCAAATCCTAAAATCCTTACAAACCCTTTTCTTCTTTATTATTTTAACTACATTCACCACCTCCGCCGTGGCCTCTTCTTCCTCCTCCACCGTCCGGCCAGTACAACCACACATCAGAAAGGCCTGCAAGCCGACGCCATATCCACGCCTTTGCGAGACTGCACTCTCCTTATACGCTTCACAAACCAAAAGAAACCAGCAAGAGCTCTGCAGGGCGGCCATGGCTTCTTCTCTCAAAGCTGCACAAAACGCCACCTCCATCATCTCCAAGCTCTCCCGTCGGAAGGTATCGCCCTATGAAGCCGAAGTCATCGGAGATTGCATCGATAATCTCAATGACTCTGTCGATGAGCTCCGTCGAGCTGCCGCTGCCATCAAAAGCTTGAGTCTAAAGTTAAGAATTCTTTTGAGTAGTATAAAAACATGGGCGAGCGCAGCACTGACCGATGTTATCACGTGCACCGACGGGTTGAGCGGCGGCTCGGGGTGGAAGGTGAGATCGAAGTTGAAGAAAGAAGTCAAGAATTGTAGTGCGAATGTAGTAAGGCAGATTAGTAATGCCTTGTTTCTCATCAACAACTTCAACTACAAATAG

Protein sequence

MHKPNRFFRERQREMESQILKSLQTLFFFIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMASSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLNDSVDELRRAAAAIKSLSLKLRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEVKNCSANVVRQISNALFLINNFNYK
BLAST of Lsi05G013180 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.8e-18
Identity = 67/198 (33.84%), Postives = 101/198 (51.01%), Query Frame = 1

Query: 22  SLQTLFFFIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKR 81
           S  TL F  +L     ++ A        P    I+ +C  T YP +CE +LS YA   + 
Sbjct: 4   SKSTLVFSALLVILAAASAA--------PANQFIKTSCTLTTYPAVCEQSLSAYAKTIQN 63

Query: 82  NQQELCRAAMASSLKAAQNATSIISKLSRRK-VSPYEAEVIGDCIDNLNDSVDELRRAAA 141
           N QEL   A+  SL   Q A + + +L++ K +   +   I DC++ + DS+D + R+  
Sbjct: 64  NPQELASTALQVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCD 123

Query: 142 AIKSLS----LKLRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEVKNCSAN 201
            +K+LS          +S+++TW SAALTD  TC DG +G     +  K+K+ V+     
Sbjct: 124 EMKNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKG---MDGKIKESVRAQVVA 183

Query: 202 VVRQISNALFLINNFNYK 215
           V R  SNAL L+NNF  K
Sbjct: 184 VARVTSNALALVNNFAAK 190

BLAST of Lsi05G013180 vs. Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 56.6 bits (135), Expect = 3.8e-07
Identity = 53/208 (25.48%), Postives = 100/208 (48.08%), Query Frame = 1

Query: 8   FRERQREMESQILKSLQTLFFFIILTTFTTSAVASSSSSTVRPVQPHIRKA-CKPTPYPR 67
           F+  ++ + S    +L  L   + +   TT+   +   +T+      I K+ C  T YP 
Sbjct: 14  FKNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPE 73

Query: 68  LCETALSLYASQTKRNQQELCRAAMASSLKAAQNATSIISKL--SRRKVSPYEAEVIGDC 127
           LC +A++    +   +Q+E+  A++  + KA ++    + KL   R+ ++P E   + DC
Sbjct: 74  LCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDC 133

Query: 128 IDNLNDSVDELRRAAAAIKSL--SLKLRILLSSIKTWASAALTDVITCTDGLSGGSGWKV 187
           ++ +++++DEL  A   +        LR     +KT  S+A+T+  TC DG S     + 
Sbjct: 134 LETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADR- 193

Query: 188 RSKLKKEVKNCSANVVRQISNALFLINN 211
             K++K +     +V    SNAL +I N
Sbjct: 194 --KVRKALLKGQVHVEHMCSNALAMIKN 218

BLAST of Lsi05G013180 vs. Swiss-Prot
Match: PME61_ARATH (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana GN=PME61 PE=2 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.5e-06
Identity = 46/161 (28.57%), Postives = 77/161 (47.83%), Query Frame = 1

Query: 47  TVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMASSLKAAQNATSIIS 106
           T +P Q  I + C  + YP LC   L  +      ++ EL   +  ++L+    A    S
Sbjct: 68  TRKPTQA-ISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSS 127

Query: 107 KLSRRKVSPYEAEVIGDCIDNLNDSVDELRRAAAAIKSLSLKLRILLSSIKTWASAALTD 166
            ++  ++ P        C++ L+DSVD L RA +++  +S       S + TW S+A+T+
Sbjct: 128 TITYTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDES--HSDVMTWLSSAMTN 187

Query: 167 VITCTDGLS--GGSGWKVRSKLKKEVKNCSANVVRQISNAL 206
             TCTDG     G G +V+ ++   VK+ S      +SN L
Sbjct: 188 HDTCTDGFDEIEGQGGEVKDQVIGAVKDLS----EMVSNCL 221

BLAST of Lsi05G013180 vs. TrEMBL
Match: A0A0A0L671_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176250 PE=4 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 3.3e-82
Identity = 176/204 (86.27%), Postives = 181/204 (88.73%), Query Frame = 1

Query: 15  MESQILKSLQTL--FFFIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETAL 74
           MESQILKS  TL  FFFIILTTFT SAV SSSSSTVRPVQPHIRKACKPTPYPRLCETAL
Sbjct: 1   MESQILKSSLTLLIFFFIILTTFTPSAV-SSSSSTVRPVQPHIRKACKPTPYPRLCETAL 60

Query: 75  SLYASQTKRNQQELCRAAMASSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLNDSV 134
           SLYASQTKRNQQELCRAAM SSLKAAQNATSIISKLSRRK+S YEAEVIGDCIDNL DSV
Sbjct: 61  SLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSV 120

Query: 135 DELRRAAAAIKSLSLK--LRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEV 194
           DELRRA+ AIKSLS    +   L+SIKTW SAA TDVITCTDGLSGGSGWKV   LKK+V
Sbjct: 121 DELRRASTAIKSLSRSKDVDFQLNSIKTWMSAAQTDVITCTDGLSGGSGWKVSKMLKKDV 180

Query: 195 KNCSANVVRQISNALFLINNFNYK 215
           KNCS NVVRQISNALFLINNFNYK
Sbjct: 181 KNCSINVVRQISNALFLINNFNYK 203

BLAST of Lsi05G013180 vs. TrEMBL
Match: M5X0K1_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011478mg PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 9.8e-26
Identity = 76/198 (38.38%), Postives = 120/198 (60.61%), Query Frame = 1

Query: 20  LKSLQTLFFFIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQT 79
           L  L  +   I +    T+   SSSS T +    +++ AC  T YP +C  +LS YAS+ 
Sbjct: 17  LTFLLPILLLIFMPNMHTTLATSSSSQTYKT---YVKTACNTTTYPLICYKSLSSYASKV 76

Query: 80  KRNQQELCRAAMASSLKAAQNATSIISKLSRRK-VSPYEAEVIGDCIDNLNDSVDELRRA 139
           K + Q+LC  A++ +LKAA+NA+S++SKL +   ++P E  V+ DCI+N+ DS+DEL+++
Sbjct: 77  KSDPQKLCTYALSVTLKAAKNASSVVSKLYKNTGLTPSEKGVVKDCIENIKDSIDELKQS 136

Query: 140 AAAIKSLSLK---LRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEVKNCSA 199
            +++ +L +    +++ L  IKTW SA +TD  TCTDG     G KV + +K  ++N   
Sbjct: 137 VSSMSNLGVSGSDVQLQLDDIKTWVSAVITDDATCTDGF---DGVKVSAAVKTAIRNSIV 196

Query: 200 NVVRQISNALFLINNFNY 214
           N  R  SNAL LI++  Y
Sbjct: 197 NAARLASNALSLIDSLIY 208

BLAST of Lsi05G013180 vs. TrEMBL
Match: B9R9Q8_RICCO (21 kDa protein, putative OS=Ricinus communis GN=RCOM_1500020 PE=4 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 8.3e-25
Identity = 81/209 (38.76%), Postives = 126/209 (60.29%), Query Frame = 1

Query: 15  MESQILKS----LQTLFF--FIILTTFTTSAVASSSSSTVRPV-QPHIRKACKPTPYPRL 74
           ME++ + S    + TLF   F++  + T +++A +++ T +   + +++ AC  T YP++
Sbjct: 1   METKTITSPCLQVSTLFLITFLVFISNTQNSLAITTTKTSKTTYKNYLKTACNSTTYPKI 60

Query: 75  CETALSLYASQTKRNQQELCRAAMASSLKAAQNATSIISKLSRRK-VSPYEAEVIGDCID 134
           C   LS Y S  + N  +LC AA+  +LKAA N ++++  LS++K +S  E  VI DC  
Sbjct: 61  CYNTLSPYTSTIQTNDLKLCNAALTITLKAASNTSAMVKSLSKQKGLSKGEVAVIKDCQY 120

Query: 135 NLNDSVDELRRAAAAIKSL--SLKLRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRS 194
            + DSVDEL+++  A+K+L  S  +   + +IKTW SAA+TD  TCTDG     G KV S
Sbjct: 121 EIEDSVDELKQSLKALKNLKGSADMEFQIDNIKTWISAAITDENTCTDGF---EGMKVSS 180

Query: 195 KLKKEVKNCSANVVRQISNALFLINNFNY 214
           K+K ++K    NV R  SNAL LIN  +Y
Sbjct: 181 KVKSKIKKSIVNVNRLTSNALALINKLSY 206

BLAST of Lsi05G013180 vs. TrEMBL
Match: F6HAT8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0022g00940 PE=4 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 2.4e-24
Identity = 83/210 (39.52%), Postives = 127/210 (60.48%), Query Frame = 1

Query: 15  MESQILKS------LQTLFFFIIL---TTFTTSAVASSSSSTVRPVQPHIRKACKPTPYP 74
           ME+ IL++      L +LFF ++L    T T+SA  S+ S T+   +  I+ +C  T YP
Sbjct: 1   MEACILRNPSAFHVLSSLFFTLLLFNSITLTSSANTSTISLTIS--KQFIKTSCNSTTYP 60

Query: 75  RLCETALSLYASQTKRNQQELCRAAMASSLKAAQNATSIISKLSRRK-VSPYEAEVIGDC 134
            +C  +LS YAS+ + +  +L   A+  SLKAA+NA+S I+ L  RK ++  E  ++ DC
Sbjct: 61  GVCYKSLSSYASKVQTSPLKLANVALTVSLKAARNASSTITLLLERKGMTRMETSIVKDC 120

Query: 135 IDNLNDSVDELRRAAAAIKSL--SLKLRILLSSIKTWASAALTDVITCTDGLSGGSGWKV 194
           ++N  D +DEL+++    KSL     +   +++IKTW SAALTD  TC+DG     G KV
Sbjct: 121 VENFGDCIDELQQSLQEFKSLGGGKNVAFQMANIKTWVSAALTDEYTCSDGF---EGQKV 180

Query: 195 RSKLKKEVKNCSANVVRQISNALFLINNFN 213
            S L++++KN  +NV +  SNAL LINN +
Sbjct: 181 SSSLQQQIKNYISNVAKITSNALALINNLS 205

BLAST of Lsi05G013180 vs. TrEMBL
Match: A0A059DJ16_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A02882 PE=4 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 2.4e-24
Identity = 76/203 (37.44%), Postives = 128/203 (63.05%), Query Frame = 1

Query: 22  SLQTLFFFIIL----TTFTTSAVASSSSSTVRPVQPH--IRKACKPTPYPRLCETALSLY 81
           +L  L  F++L    +  T++A +SSSSS+ R  +    I+ +C  T YP+LC   LS Y
Sbjct: 5   NLHFLAIFLLLVANMSAITSAAASSSSSSSSRNGKDSNFIKTSCSTTTYPKLCNKYLSSY 64

Query: 82  ASQTKRNQQELCRAAMASSLKAAQNATSIISKLS-RRKVSPYEAEVIGDCIDNLNDSVDE 141
           AS  + N  +LC  A++ SL+AA+N +S+++ LS +R ++  EA ++ DCI+NL D++DE
Sbjct: 65  ASVVRSNPWKLCNCALSVSLQAARNTSSLVTTLSKKRSLTKAEASIVKDCIENLGDTIDE 124

Query: 142 LRRAAAAIKSLSLKLR----ILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEV 201
           L+++   + ++S   +      ++++KTW SAA+TD ITCTDG++     KV + +K+++
Sbjct: 125 LKQSIKTMANVSSAAKDDVAFQMANVKTWVSAAITDEITCTDGIAER---KVSAGVKRKI 184

Query: 202 KNCSANVVRQISNALFLINNFNY 214
           ++   NV R  SN L LIN+  Y
Sbjct: 185 QSYIENVARITSNGLSLINSLKY 204

BLAST of Lsi05G013180 vs. TAIR10
Match: AT4G25250.1 (AT4G25250.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 96.7 bits (239), Expect = 1.9e-20
Identity = 62/192 (32.29%), Postives = 106/192 (55.21%), Query Frame = 1

Query: 25  TLFFFIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQ 84
           TL  FI  + F  +A  ++   T +    +++ AC  T YP +C   LS Y+S  K +  
Sbjct: 10  TLMVFINSSNFPKTA--ATPPGTYQNHTTYVKTACNSTTYPTMCYNCLSSYSSTIKSDPI 69

Query: 85  ELCRAAMASSLKAAQNATSIISKL--SRRKVSPYEAEVIGDCIDNLNDSVDELRRAAAAI 144
           +LC  ++  ++K+A+NAT ++S L    +    +E  ++ DC+D + D++DEL++A A +
Sbjct: 70  KLCTTSLNLNVKSAKNATLVVSNLLQKAKAAKSHEVSILKDCVDEMKDTIDELKQAVAEM 129

Query: 145 KSL---SLKLRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEVKNCSANVVR 204
           K +          L ++KTW S+ALTD  TCTDG   G   +V  + KK+VK   + + +
Sbjct: 130 KYVRGGGKTTEEHLKNVKTWVSSALTDEGTCTDGFEEG---RVNVETKKKVKKAISELSK 189

Query: 205 QISNALFLINNF 212
             SN L L+ ++
Sbjct: 190 TTSNTLALLTHY 196

BLAST of Lsi05G013180 vs. TAIR10
Match: AT5G62350.1 (AT5G62350.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 96.7 bits (239), Expect = 1.9e-20
Identity = 65/187 (34.76%), Postives = 103/187 (55.08%), Query Frame = 1

Query: 31  ILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAA 90
           +L+   T+A A+S +   +     I+ +CK T YP LC  +LS+YA+  + + + L   A
Sbjct: 15  LLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAETA 74

Query: 91  MASSLKAAQNATSIISKLSRRK-VSPYEAEVIGDCIDNLNDSVDELRRAAAAIKSLSL-- 150
           +A +L  AQ+    +S+L+R K +   E E I DC++ +ND+VD L ++   +K      
Sbjct: 75  IAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRLTKSVQELKLCGSAK 134

Query: 151 ---KLRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEVKNCSANVVRQISNA 210
              +    +S+ +TW SAALTD  TC+DG SG     +  ++K  V+    NV  + SNA
Sbjct: 135 DQDQFAYHMSNAQTWTSAALTDENTCSDGFSGR---VMDGRIKNSVRARIMNVGHETSNA 194

Query: 211 LFLINNF 212
           L LIN F
Sbjct: 195 LSLINAF 198

BLAST of Lsi05G013180 vs. TAIR10
Match: AT5G62360.1 (AT5G62360.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 94.0 bits (232), Expect = 1.2e-19
Identity = 60/185 (32.43%), Postives = 104/185 (56.22%), Query Frame = 1

Query: 28  FFIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQELC 87
           F II+         +++++T       ++ +C  T YPRLC ++LS +AS  + + + + 
Sbjct: 17  FLIIIAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMA 76

Query: 88  RAAMASSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLNDSVDELRRAAAAIKSLS- 147
            AA+  +L +A+  ++++ +LS  ++ P E   + DC++ L D+++ELR++   +  LS 
Sbjct: 77  HAALNITLASAKVTSAMMVRLSNSRLKPKEVSAMRDCVEELGDTLEELRKSIGEMCQLSG 136

Query: 148 LKLRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEVKNCSANVVRQISNALF 207
               + +S I+TW SAALTDV TCTDG  G     +  K+K  V+     +    SNAL 
Sbjct: 137 SNYEVYISDIQTWVSAALTDVNTCTDGFEGED---MDGKVKVLVRGRILVIAHLTSNALA 196

Query: 208 LINNF 212
           LIN+F
Sbjct: 197 LINHF 198

BLAST of Lsi05G013180 vs. TAIR10
Match: AT5G51520.1 (AT5G51520.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 93.6 bits (231), Expect = 1.6e-19
Identity = 69/209 (33.01%), Postives = 112/209 (53.59%), Query Frame = 1

Query: 17  SQILKSLQTLFFFIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYA 76
           SQ+L SL  +F F   T    ++ ++SS S     +  ++ AC  T YP  C  +LS Y+
Sbjct: 2   SQVLYSLTIVFVFFASTNIQKTSGSASSYSQNH--KTFVKTACNSTTYPDKCYKSLSSYS 61

Query: 77  SQTKRNQQELCRAAMASSLKAAQNATSIISKL----------SRRKVSPYEAEVIGDCID 136
           S  K +  +LC  A+  ++K+A+ ATS++SKL           + K+ P EA ++ DC++
Sbjct: 62  SNIKSDPIKLCTTALNLNVKSAKEATSVVSKLLKMSQKSTAGRKGKMLP-EALILKDCLE 121

Query: 137 NLNDSVDELRRAAAAIKSL--SLKLRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRS 196
            + D++ EL++A   +K+L     +   +++++TW S+ALTD  TCTDG       KV  
Sbjct: 122 EMKDTIIELKQAITEMKNLQDGGSMAEHITNVRTWVSSALTDEGTCTDGF---EEVKVNK 181

Query: 197 KLKKEVKNCSANVVRQISNALFLINNFNY 214
           + KK+V      +    SN L LI N  Y
Sbjct: 182 ETKKKVNKVVEELATTTSNTLALITNLRY 204

BLAST of Lsi05G013180 vs. TAIR10
Match: AT5G20740.1 (AT5G20740.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 86.3 bits (212), Expect = 2.5e-17
Identity = 63/192 (32.81%), Postives = 97/192 (50.52%), Query Frame = 1

Query: 29  FIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCR 88
           F++   F     AS+        +  +  +C+   YP LC   LS Y+  T  N+++L +
Sbjct: 8   FLVAIAFAVIFTASTVHGRHNGAEDIVHSSCEHASYPSLCVRTLSSYSGPTITNRRDLAQ 67

Query: 89  AAMASSLKAAQNATSIISKLSRRKVSPYEAE--VIGDCIDNLNDSVDELRRAAAAIKSLS 148
           AA+  SL  AQ+A   ++ + R  V   + E   + DC++ + DSVDEL R    +K L 
Sbjct: 68  AAIKISLSHAQSAAKKLAVV-RDSVGKKKQEKAALVDCVEMIGDSVDELSRTLGVLKHLR 127

Query: 149 L------KLRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEVKNCSANVVRQ 208
           +      + R  +S+ +TWASAALTD  TC DG  G        ++K EVK     V R 
Sbjct: 128 VSGGSAKEFRWQMSNAQTWASAALTDDDTCLDGFQGMDD----GEIKTEVKQWMTKVARV 187

Query: 209 ISNALFLINNFN 213
            SNAL+++N  +
Sbjct: 188 TSNALYMVNQLD 194

BLAST of Lsi05G013180 vs. NCBI nr
Match: gi|449460810|ref|XP_004148137.1| (PREDICTED: 21 kDa protein-like [Cucumis sativus])

HSP 1 Score: 312.8 bits (800), Expect = 4.7e-82
Identity = 176/204 (86.27%), Postives = 181/204 (88.73%), Query Frame = 1

Query: 15  MESQILKSLQTL--FFFIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETAL 74
           MESQILKS  TL  FFFIILTTFT SAV SSSSSTVRPVQPHIRKACKPTPYPRLCETAL
Sbjct: 1   MESQILKSSLTLLIFFFIILTTFTPSAV-SSSSSTVRPVQPHIRKACKPTPYPRLCETAL 60

Query: 75  SLYASQTKRNQQELCRAAMASSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLNDSV 134
           SLYASQTKRNQQELCRAAM SSLKAAQNATSIISKLSRRK+S YEAEVIGDCIDNL DSV
Sbjct: 61  SLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSV 120

Query: 135 DELRRAAAAIKSLSLK--LRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEV 194
           DELRRA+ AIKSLS    +   L+SIKTW SAA TDVITCTDGLSGGSGWKV   LKK+V
Sbjct: 121 DELRRASTAIKSLSRSKDVDFQLNSIKTWMSAAQTDVITCTDGLSGGSGWKVSKMLKKDV 180

Query: 195 KNCSANVVRQISNALFLINNFNYK 215
           KNCS NVVRQISNALFLINNFNYK
Sbjct: 181 KNCSINVVRQISNALFLINNFNYK 203

BLAST of Lsi05G013180 vs. NCBI nr
Match: gi|659077238|ref|XP_008439099.1| (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 308.9 bits (790), Expect = 6.8e-81
Identity = 172/203 (84.73%), Postives = 181/203 (89.16%), Query Frame = 1

Query: 15  MESQILKSLQTLFFF-IILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALS 74
           MESQILKS  TLFFF IILTTFT SAV +SSSSTVRPV+PHIRKACKPTPYPRLCETALS
Sbjct: 1   MESQILKSSLTLFFFFIILTTFTPSAV-ASSSSTVRPVKPHIRKACKPTPYPRLCETALS 60

Query: 75  LYASQTKRNQQELCRAAMASSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLNDSVD 134
           LYASQTKRNQQELCRAAM SSLKAAQNATSIISKLSRRK+S YE EVIGDCID+L DSVD
Sbjct: 61  LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYETEVIGDCIDDLKDSVD 120

Query: 135 ELRRAAAAIKSLSLK--LRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEVK 194
           ELRRA+ AIKSLS    +   L+SIKTW SAALTD+ITCTDGLSGGSGWKV  K+KKEVK
Sbjct: 121 ELRRASTAIKSLSRSKDVDFQLNSIKTWTSAALTDIITCTDGLSGGSGWKVNKKVKKEVK 180

Query: 195 NCSANVVRQISNALFLINNFNYK 215
           NCS NVVRQISNAL LINNFNYK
Sbjct: 181 NCSINVVRQISNALSLINNFNYK 202

BLAST of Lsi05G013180 vs. NCBI nr
Match: gi|470146900|ref|XP_004309057.1| (PREDICTED: 21 kDa protein-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 128.6 bits (322), Expect = 1.3e-26
Identity = 78/190 (41.05%), Postives = 119/190 (62.63%), Query Frame = 1

Query: 26  LFFFIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQE 85
           LF  ++   F  +  A+SSSS  R  + +++ AC  T YP++C  +LS YA+  K N Q+
Sbjct: 7   LFALLLFILFINTTTATSSSS--RTYKTYVKTACNSTTYPQICYKSLSSYATSIKSNPQK 66

Query: 86  LCRAAMASSLKAAQNATSIISKLSRRK-VSPYEAEVIGDCIDNLNDSVDELRRAAAAIKS 145
           LC  A++ +LKAA+ + S +SKL+++K ++  EA V+G CI+ + DSVDEL+++  A+K+
Sbjct: 67  LCSYALSVTLKAAKTSASTVSKLAKKKGITKAEAGVVGACIETIKDSVDELKQSVTAMKN 126

Query: 146 L-SLKLRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEVKNCSANVVRQISN 205
           L    ++  L +IKTW SA+LTD  TCTDG       KV + +K  ++     V R  SN
Sbjct: 127 LGGADMQYQLDNIKTWVSASLTDEDTCTDGF---DEQKVSTAVKNTIRKSILTVARLASN 186

Query: 206 ALFLINNFNY 214
           AL LI+N NY
Sbjct: 187 ALSLIDNLNY 191

BLAST of Lsi05G013180 vs. NCBI nr
Match: gi|657945059|ref|XP_008377851.1| (PREDICTED: 21 kDa protein [Malus domestica])

HSP 1 Score: 128.3 bits (321), Expect = 1.7e-26
Identity = 77/199 (38.69%), Postives = 124/199 (62.31%), Query Frame = 1

Query: 19  ILKSLQTLFFFIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQ 78
           +L  L  +   II+T   T+   SSSS T +    +++ AC  T YP +C  +LS YAS+
Sbjct: 16  VLTFLLPIJLLIIMTNXQTTVATSSSSQTYKT---YVKTACNSTTYPLVCYQSLSSYASK 75

Query: 79  TKRNQQELCRAAMASSLKAAQNATSIISKLSRRK-VSPYEAEVIGDCIDNLNDSVDELRR 138
            K N ++LC  A++ +L+A++NA+S +SKLS+R  ++P E  +I DC++++ +S+DEL+ 
Sbjct: 76  VKSNPRKLCIYALSVTLQASKNASSXVSKLSKRAGLTPTEKGIINDCLESIKESIDELKD 135

Query: 139 AAAAIKSLSLK---LRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEVKNCS 198
           + +++K+L +K   ++  L  IKTW SA +TD +TCTDG     G KV + +K  + N  
Sbjct: 136 SLSSMKNLGVKGADMQAQLDDIKTWVSAVITDDVTCTDGF---DGLKVSTAVKTPINNSI 195

Query: 199 ANVVRQISNALFLINNFNY 214
             V R  SNAL LI++  Y
Sbjct: 196 VYVARLASNALSLIDSLXY 208

BLAST of Lsi05G013180 vs. NCBI nr
Match: gi|645257369|ref|XP_008234383.1| (PREDICTED: 21 kDa protein-like [Prunus mume])

HSP 1 Score: 125.9 bits (315), Expect = 8.2e-26
Identity = 77/198 (38.89%), Postives = 119/198 (60.10%), Query Frame = 1

Query: 20  LKSLQTLFFFIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQT 79
           L  L  +   I +    T+   SSSS T +    +++ AC  T YP +C  +LS YAS+ 
Sbjct: 17  LTFLLPILLLIFMPNMHTTLATSSSSQTYKS---YVKTACNTTTYPLICYKSLSSYASKV 76

Query: 80  KRNQQELCRAAMASSLKAAQNATSIISKLSRRK-VSPYEAEVIGDCIDNLNDSVDELRRA 139
           K +  +LC  A++ +LKAA+NA+S++SKL +   ++P E  V+ DCIDN+ DS+DEL+++
Sbjct: 77  KSDPHKLCTYALSVTLKAAKNASSVVSKLYKNTGLTPSEKGVVKDCIDNIKDSIDELKQS 136

Query: 140 AAAIKSLSLK---LRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEVKNCSA 199
            +++ +L +    +++ L  IKTW SA +TD  TCTDG     G KV + +K  +KN   
Sbjct: 137 VSSMSNLGVSGSDVQLQLDDIKTWVSAVITDDATCTDGF---DGVKVSTAVKTAIKNSIV 196

Query: 200 NVVRQISNALFLINNFNY 214
           N  R  SNAL LI++  Y
Sbjct: 197 NAARLASNALSLIDSLIY 208

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
21KD_DAUCA2.8e-1833.8421 kDa protein OS=Daucus carota PE=2 SV=1[more]
PME2_ARATH3.8e-0725.48Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2[more]
PME61_ARATH1.5e-0628.57Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana GN=P... [more]
Match NameE-valueIdentityDescription
A0A0A0L671_CUCSA3.3e-8286.27Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176250 PE=4 SV=1[more]
M5X0K1_PRUPE9.8e-2638.38Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011478mg PE=4 SV=1[more]
B9R9Q8_RICCO8.3e-2538.7621 kDa protein, putative OS=Ricinus communis GN=RCOM_1500020 PE=4 SV=1[more]
F6HAT8_VITVI2.4e-2439.52Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0022g00940 PE=4 SV=... [more]
A0A059DJ16_EUCGR2.4e-2437.44Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A02882 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G25250.11.9e-2032.29 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G62350.11.9e-2034.76 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G62360.11.2e-1932.43 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G51520.11.6e-1933.01 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G20740.12.5e-1732.81 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449460810|ref|XP_004148137.1|4.7e-8286.27PREDICTED: 21 kDa protein-like [Cucumis sativus][more]
gi|659077238|ref|XP_008439099.1|6.8e-8184.73PREDICTED: 21 kDa protein-like [Cucumis melo][more]
gi|470146900|ref|XP_004309057.1|1.3e-2641.05PREDICTED: 21 kDa protein-like [Fragaria vesca subsp. vesca][more]
gi|657945059|ref|XP_008377851.1|1.7e-2638.69PREDICTED: 21 kDa protein [Malus domestica][more]
gi|645257369|ref|XP_008234383.1|8.2e-2638.89PREDICTED: 21 kDa protein-like [Prunus mume][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR006501Pectinesterase_inhib_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G013180.1Lsi05G013180.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 54..211
score: 5.0
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 55..205
score: 6.9
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 50..207
score: 1.6
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 22..210
score: 3.6
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 53..211
score: 5.89
NoneNo IPR availableunknownCoilCoilcoord: 125..145
scor
NoneNo IPR availablePANTHERPTHR31080FAMILY NOT NAMEDcoord: 15..210
score: 3.0
NoneNo IPR availablePANTHERPTHR31080:SF15SUBFAMILY NOT NAMEDcoord: 15..210
score: 3.0

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi05G013180Silver-seed gourdcarlsiB841
Lsi05G013180Cucumber (Chinese Long) v3cuclsiB245
Lsi05G013180Cucumber (Chinese Long) v3cuclsiB405
Lsi05G013180Cucumber (Chinese Long) v3cuclsiB399
Lsi05G013180Watermelon (97103) v2lsiwmbB315
Lsi05G013180Bottle gourd (USVL1VR-Ls)lsilsiB008
Lsi05G013180Cucumber (Gy14) v1cgylsiB416
Lsi05G013180Cucumber (Gy14) v1cgylsiB490
Lsi05G013180Cucurbita maxima (Rimu)cmalsiB288
Lsi05G013180Cucurbita maxima (Rimu)cmalsiB312
Lsi05G013180Cucurbita maxima (Rimu)cmalsiB580
Lsi05G013180Cucurbita maxima (Rimu)cmalsiB688
Lsi05G013180Cucurbita moschata (Rifu)cmolsiB277
Lsi05G013180Cucurbita moschata (Rifu)cmolsiB300
Lsi05G013180Cucurbita moschata (Rifu)cmolsiB560
Lsi05G013180Cucurbita moschata (Rifu)cmolsiB570
Lsi05G013180Cucurbita moschata (Rifu)cmolsiB681
Lsi05G013180Cucurbita pepo (Zucchini)cpelsiB172
Lsi05G013180Cucurbita pepo (Zucchini)cpelsiB624
Lsi05G013180Wild cucumber (PI 183967)cpilsiB208
Lsi05G013180Wild cucumber (PI 183967)cpilsiB379
Lsi05G013180Wild cucumber (PI 183967)cpilsiB384
Lsi05G013180Cucumber (Chinese Long) v2culsiB210
Lsi05G013180Cucumber (Chinese Long) v2culsiB376
Lsi05G013180Cucumber (Chinese Long) v2culsiB380
Lsi05G013180Melon (DHL92) v3.5.1lsimeB340
Lsi05G013180Melon (DHL92) v3.5.1lsimeB379
Lsi05G013180Watermelon (Charleston Gray)lsiwcgB322
Lsi05G013180Watermelon (Charleston Gray)lsiwcgB328
Lsi05G013180Watermelon (Charleston Gray)lsiwcgB336
Lsi05G013180Watermelon (97103) v1lsiwmB373
Lsi05G013180Cucumber (Gy14) v2cgyblsiB342
Lsi05G013180Cucumber (Gy14) v2cgyblsiB346
Lsi05G013180Melon (DHL92) v3.6.1lsimedB356
Lsi05G013180Melon (DHL92) v3.6.1lsimedB357