Carg08008 (gene) Silver-seed gourd

Overview
NameCarg08008
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Description21 kDa protein-like
LocationCucurbita_argyrosperma_scaffold_050 : 165164 .. 166087 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATTTCAAATCCTAAAATCGTTACTAACCCTTTTCTTTTTCGTTTCAATTACGATTACCACCTCCGTCGTGGCTTCTTCTTCTTCCATTACCCGTCCGGTGAAACCATACATCATAAAGGCTTGCAAAACGACGCCGTATCCACGCCTTTGCGAGATCTCTCTCACTTTACATGCGTCACAAACCAAAAAAACACAACAAGAGTTCTGCAGAGCGACCATGGCTTCTTCTCTCAAAACGACACGAGATGCCACCTCCATAATCTCCAAGCTATCTCGCCAAAAGATGTCTACCTATGAAGCTAAGGTCATTAGAGATTGTGTTGATAATCTTAAAGACTCTACCGACGAGCTCCGTCGAGCTATTGCCGCAATCAAAAGCTTGAGTCGTGCTAAAGATGCAAACTTTCAGGTTATTTAAACTTGTACATAACCCCAAAAGCTTTAAATTTATATGTTTTCTTAAATTTTATCTCAAATTCATATTTTAAACGCCATTCTACGTCATCTAAAAAAAATTGAAATATCTTTAGACATGATCTTCGCACATTTATGGGTTGTCCTTTAAATACGAAAGTCAACATAGAGACCTAAAAATATATATTTAATCAAATAAGTTAACCTTTTTTATAAATGAAAGTGGGTTGATTATCAATTTTAATTAAACCCTTCAAATAATTTATGGTAGATTGGGTTAAAGTTAATATTTTTATTCATACTGTGAACACAGTTACATAGTATCAAAACATGGATGAGTGCTGCAGAGACCGATGCTATGACTTGCAGCGATGGGTTGAGCGGCGGCGCTGGTCGGAAGGTGAGGTCGAAGGTGAAGCAAGAGGTGAAGAAGAGTGTTGCGGTTGTGGTGAGACATATCAGTAATGCCTTGTCCCTCCTCAACACCTTCAAATACAAATCCTGA

mRNA sequence

ATGGAATTTCAAATCCTAAAATCGTTACTAACCCTTTTCTTTTTCGTTTCAATTACGATTACCACCTCCGTCGTGGCTTCTTCTTCTTCCATTACCCGTCCGGTGAAACCATACATCATAAAGGCTTGCAAAACGACGCCGTATCCACGCCTTTGCGAGATCTCTCTCACTTTACATGCGTCACAAACCAAAAAAACACAACAAGAGTTCTGCAGAGCGACCATGGCTTCTTCTCTCAAAACGACACGAGATGCCACCTCCATAATCTCCAAGCTATCTCGCCAAAAGATGTCTACCTATGAAGCTAAGGTCATTAGAGATTGTGTTGATAATCTTAAAGACTCTACCGACGAGCTCCGTCGAGCTATTGCCGCAATCAAAAGCTTGAGTCGTGCTAAAGATGCAAACTTTCAGTTACATAGTATCAAAACATGGATGAGTGCTGCAGAGACCGATGCTATGACTTGCAGCGATGGGTTGAGCGGCGGCGCTGGTCGGAAGGTGAGGTCGAAGGTGAAGCAAGAGGTGAAGAAGAGTGTTGCGGTTGTGGTGAGACATATCAGTAATGCCTTGTCCCTCCTCAACACCTTCAAATACAAATCCTGA

Coding sequence (CDS)

ATGGAATTTCAAATCCTAAAATCGTTACTAACCCTTTTCTTTTTCGTTTCAATTACGATTACCACCTCCGTCGTGGCTTCTTCTTCTTCCATTACCCGTCCGGTGAAACCATACATCATAAAGGCTTGCAAAACGACGCCGTATCCACGCCTTTGCGAGATCTCTCTCACTTTACATGCGTCACAAACCAAAAAAACACAACAAGAGTTCTGCAGAGCGACCATGGCTTCTTCTCTCAAAACGACACGAGATGCCACCTCCATAATCTCCAAGCTATCTCGCCAAAAGATGTCTACCTATGAAGCTAAGGTCATTAGAGATTGTGTTGATAATCTTAAAGACTCTACCGACGAGCTCCGTCGAGCTATTGCCGCAATCAAAAGCTTGAGTCGTGCTAAAGATGCAAACTTTCAGTTACATAGTATCAAAACATGGATGAGTGCTGCAGAGACCGATGCTATGACTTGCAGCGATGGGTTGAGCGGCGGCGCTGGTCGGAAGGTGAGGTCGAAGGTGAAGCAAGAGGTGAAGAAGAGTGTTGCGGTTGTGGTGAGACATATCAGTAATGCCTTGTCCCTCCTCAACACCTTCAAATACAAATCCTGA

Protein sequence

MEFQILKSLLTLFFFVSITITTSVVASSSSITRPVKPYIIKACKTTPYPRLCEISLTLHASQTKKTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLKDSTDELRRAIAAIKSLSRAKDANFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSVAVVVRHISNALSLLNTFKYKS
Homology
[Close]
BLAST of Carg08008 vs. NCBI nr
Match: XP_022942851.1 (21 kDa protein-like [Cucurbita moschata])

HSP 1 Score: 365.2 bits (936), Expect = 1.5e-97
Identity = 198/201 (98.51%), Postives = 200/201 (99.50%), Query Frame = 0 

Query: 1   MEFQILKSLLTLFFFVSITITTSVVASSSSITRPVKPYIIKACKTTPYPRLCEISLTLHA 60
           MEFQILKSLLTLFFFVSITITTSVVASSSSITRPVKPYIIKACKTTPYPRLCEISLTLHA
Sbjct: 1   MEFQILKSLLTLFFFVSITITTSVVASSSSITRPVKPYIIKACKTTPYPRLCEISLTLHA 60

Query: 61  SQTKKTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLKDSTDELR 120
           SQTK+TQQEFCRATM SSLKTTRDATSIISKLSRQK+STYEAKVIRDCVDNLKDSTDELR
Sbjct: 61  SQTKRTQQEFCRATMTSSLKTTRDATSIISKLSRQKISTYEAKVIRDCVDNLKDSTDELR 120

Query: 121 RAIAAIKSLSRAKDANFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 180
           RAIAAIKSLSRAKDANFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV
Sbjct: 121 RAIAAIKSLSRAKDANFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 180

Query: 181 AVVVRHISNALSLLNTFKYKS 202
           AVVVRHISNALSLLNTFKYKS
Sbjct: 181 AVVVRHISNALSLLNTFKYKS 201

[Close]
BLAST of Carg08008 vs. NCBI nr
Match: XP_023542401.1 (21 kDa protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 357.8 bits (917), Expect = 2.3e-95
Identity = 193/201 (96.02%), Postives = 198/201 (98.51%), Query Frame = 0 

Query: 1   MEFQILKSLLTLFFFVSITITTSVVASSSSITRPVKPYIIKACKTTPYPRLCEISLTLHA 60
           MEFQILKSLLTLFFFVSIT TTSVVASSSS+TRPVKPYI KACKTTPYPRLCEISLTLHA
Sbjct: 1   MEFQILKSLLTLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHA 60

Query: 61  SQTKKTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLKDSTDELR 120
           SQTK+TQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNL+DSTDEL+
Sbjct: 61  SQTKRTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLQDSTDELQ 120

Query: 121 RAIAAIKSLSRAKDANFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 180
           RAIAAIK+LSRAKD NFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV
Sbjct: 121 RAIAAIKNLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 180

Query: 181 AVVVRHISNALSLLNTFKYKS 202
           AVVVRHISNALSLLNTFKYKS
Sbjct: 181 AVVVRHISNALSLLNTFKYKS 201

[Close]
BLAST of Carg08008 vs. NCBI nr
Match: XP_022987359.1 (21 kDa protein-like [Cucurbita maxima])

HSP 1 Score: 350.9 bits (899), Expect = 2.9e-93
Identity = 191/201 (95.02%), Postives = 193/201 (96.02%), Query Frame = 0 

Query: 1   MEFQILKSLLTLFFFVSITITTSVVASSSSITRPVKPYIIKACKTTPYPRLCEISLTLHA 60
           MEFQILKSLLTLFFFVSIT TTSVVASSSSI RPVKPYI KACKTTPYPRLCEISLTLHA
Sbjct: 1   MEFQILKSLLTLFFFVSITFTTSVVASSSSIIRPVKPYIKKACKTTPYPRLCEISLTLHA 60

Query: 61  SQTKKTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLKDSTDELR 120
           SQTKKTQQEFCRA MASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLKDSTDEL 
Sbjct: 61  SQTKKTQQEFCRAAMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLKDSTDELH 120

Query: 121 RAIAAIKSLSRAKDANFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 180
           RAIA  KSLSRAKDANFQLHSIKTW+SAAETDAMTCSDGLSGGAGRKVRSKVKQEV+KSV
Sbjct: 121 RAIAGFKSLSRAKDANFQLHSIKTWVSAAETDAMTCSDGLSGGAGRKVRSKVKQEVRKSV 180

Query: 181 AVVVRHISNALSLLNTFKYKS 202
           AVVVRHISNALSLL TFKYKS
Sbjct: 181 AVVVRHISNALSLLTTFKYKS 201

[Close]
BLAST of Carg08008 vs. NCBI nr
Match: XP_022985512.1 (pectinesterase inhibitor 4-like [Cucurbita maxima])

HSP 1 Score: 236.9 bits (603), Expect = 6.0e-59
Identity = 132/196 (67.35%), Postives = 157/196 (80.10%), Query Frame = 0 

Query: 6   LKSLLTLFFFVSI-TITTSVVASSSSITRPVKPYIIKACKTTPYPRLCEISLTLHASQTK 65
           +KSLL LFFF+ I T T S VA+SSS  +PVKP+I KAC+ TPYPRLCE +L+L++SQTK
Sbjct: 1   MKSLLILFFFILIATFTISAVAASSSAIQPVKPHIRKACRPTPYPRLCETALSLYSSQTK 60

Query: 66  KTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLKDSTDELRRAIA 125
           +TQQE CRA + SSLK  ++ATSIISKLS +K+S YEA+VI DC DNLKDS  ELRRA  
Sbjct: 61  RTQQELCRAAVVSSLKAAQNATSIISKLSHRKVSPYEAEVIGDCTDNLKDSIYELRRATT 120

Query: 126 AIKSLSRAKDANFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSVAVVV 185
           AIKSL+RA D  FQL SIKTW+SAAET+ MTC+DGLSGGAG KVRS VK+EVKK    V+
Sbjct: 121 AIKSLNRADDVEFQLSSIKTWLSAAETNIMTCTDGLSGGAGWKVRSMVKKEVKKCSVNVI 180

Query: 186 RHISNALSLLNTFKYK 201
           R  SNALSL++  KYK
Sbjct: 181 RQTSNALSLISNLKYK 196

[Close]
BLAST of Carg08008 vs. NCBI nr
Match: XP_008439099.1 (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 225.3 bits (573), Expect = 1.8e-55
Identity = 119/168 (70.83%), Postives = 138/168 (82.14%), Query Frame = 0 

Query: 33  RPVKPYIIKACKTTPYPRLCEISLTLHASQTKKTQQEFCRATMASSLKTTRDATSIISKL 92
           RPVKP+I KACK TPYPRLCE +L+L+ASQTK+ QQE CRA M SSLK  ++ATSIISKL
Sbjct: 35  RPVKPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKL 94

Query: 93  SRQKMSTYEAKVIRDCVDNLKDSTDELRRAIAAIKSLSRAKDANFQLHSIKTWMSAAETD 152
           SR+KMS YE +VI DC+D+LKDS DELRRA  AIKSLSR+KD +FQL+SIKTW SAA TD
Sbjct: 95  SRRKMSAYETEVIGDCIDDLKDSVDELRRASTAIKSLSRSKDVDFQLNSIKTWTSAALTD 154

Query: 153 AMTCSDGLSGGAGRKVRSKVKQEVKKSVAVVVRHISNALSLLNTFKYK 201
            +TC+DGLSGG+G KV  KVK+EVK     VVR ISNALSL+N F YK
Sbjct: 155 IITCTDGLSGGSGWKVNKKVKKEVKNCSINVVRQISNALSLINNFNYK 202

[Close]
BLAST of Carg08008 vs. TAIR10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 101.7 bits (252), Expect = 5.5e-22
Identity = 65/201 (32.34%), Postives = 111/201 (55.22%), Query Frame = 0 

Query: 1   MEFQILKSLLTLFFFVSITITTSVVASSSSITRPVKPYIIKACKTTPYPRLCEISLTLHA 60
           M  Q L  LL++ + +S+ +T +  AS +  ++    +I  +CKTT YP LC  SL+++A
Sbjct: 1   MAKQYLFVLLSISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYA 60

Query: 61  SQTKKTQQEFCRATMASSLKTTRDATSIISKLSRQK-MSTYEAKVIRDCVDNLKDSTDEL 120
           +  + + +      +A +L   +     +S+L+R K +   E + I+DCV+ + D+ D L
Sbjct: 61  NDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRL 120

Query: 121 RRAIAAIKSLSRAKDAN---FQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEV 180
            +++  +K    AKD +   + + + +TW SAA TD  TCSDG S   GR +  ++K  V
Sbjct: 121 TKSVQELKLCGSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFS---GRVMDGRIKNSV 180

Query: 181 KKSVAVVVRHISNALSLLNTF 198
           +  +  V    SNALSL+N F
Sbjct: 181 RARIMNVGHETSNALSLINAF 198

[Close]
BLAST of Carg08008 vs. TAIR10
Match: AT1G62770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 82.4 bits (202), Expect = 3.5e-16
Identity = 65/204 (31.86%), Postives = 113/204 (55.39%), Query Frame = 0 

Query: 6   LKSLLTLFFFVSITITTSVVASSSSITRPVKPYIIKACKTTPYPRLCEISLTLHASQTK- 65
           LK+ + L   +SITI  S  +S++        +I+ +C+TT YP LC  +L+ +A++ + 
Sbjct: 3   LKNTIFLVILLSITILQS--SSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRH 62

Query: 66  KTQQEFCRATMASSLKTTRDATSIISKLSRQ--KMSTYEAKVIRDCVDNLKDSTDELRRA 125
              Q+  +  +  SL   +  T  ++KL+++  K    E   I+DC++ L +S D L + 
Sbjct: 63  NNDQDLAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQ- 122

Query: 126 IAAIKSLSRA------KDANFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEV 185
             ++K L+RA      +D  +++ +++TW+SAA TD  TC DG S    R +  KVK+ +
Sbjct: 123 --SVKELARAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFS---ERAMGGKVKRLI 182

Query: 186 KKSVAVVVRHISNALSLLNTFKYK 201
           +  V  V +  SNAL+L+N F  K
Sbjct: 183 RYKVVHVAQVTSNALALVNQFAEK 198

[Close]
BLAST of Carg08008 vs. TAIR10
Match: AT1G14890.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 80.1 bits (196), Expect = 1.7e-15
Identity = 52/164 (31.71%), Postives = 88/164 (53.66%), Query Frame = 0 

Query: 38  YIIKACKTTPYPRLCEISLTLHASQTKKTQQEFCRATMASSLKTTRDATSIISKLSRQKM 97
           +I  +C TT YP +C  SL  +AS  +       +  +  SL   +   + +SKLSR+  
Sbjct: 58  FIRTSCNTTLYPDVCYTSLAGYASAVQDNPARLAKLAIGVSLSRAKYTAAYLSKLSRRAA 117

Query: 98  STYEAKVIRDCVDNLKDSTDELRRAIAAIKSLSRAKDAN----FQLHSIKTWMSAAETDA 157
           S      + DCV N+ D+ D++R ++  ++ ++  +  +    FQ+ +++TWMSAA TD 
Sbjct: 118 SA----AVHDCVSNVGDAVDQMRGSLRQLREMNHRRPGDPAFRFQMSNVQTWMSAALTDE 177

Query: 158 MTCSDGLSGGAGRKVRSKVKQEVKKSVAVVVRHISNALSLLNTF 198
            TC+DG++         + K  +   VA V R  SNAL+L+NT+
Sbjct: 178 ETCTDGVT---EEMEDGETKTAICDRVADVKRFTSNALALVNTY 214

[Close]
BLAST of Carg08008 vs. TAIR10
Match: AT3G47380.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 79.7 bits (195), Expect = 2.2e-15
Identity = 60/200 (30.00%), Postives = 103/200 (51.50%), Query Frame = 0 

Query: 11  TLFFFVSITITTSVVASSSSITRPVKP------YIIKACKTTPYPRLCEISLTLHASQTK 70
           TLF F+   ++T+++ +SSS  R          +I  +CK T YP +C  SLT +A+  +
Sbjct: 8   TLFLFL---LSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQ 67

Query: 71  KTQQEFCRATMASSLKTTRDATSIISKLSR-QKMSTYEAKVIRDCVDNLKDSTDELRRAI 130
            + +      +  ++   +     + +L R   +   E + ++DC++ + D+ D L  +I
Sbjct: 68  TSPRRLAETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSI 127

Query: 131 AAIKSLSRAKDAN---FQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 190
             +K    AK  +   F + + +TW SAA T+A TCSDG    AGR +  +VK  V+  +
Sbjct: 128 HEVKMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGF---AGRVMDGRVKNSVRARI 187

Query: 191 AVVVRHISNALSLLNTFKYK 201
             + R  SNAL+L+N F  K
Sbjct: 188 LNLGRGTSNALALINAFAKK 201

[Close]
BLAST of Carg08008 vs. TAIR10
Match: AT4G12390.1 (pectin methylesterase inhibitor 1)

HSP 1 Score: 75.5 bits (184), Expect = 4.2e-14
Identity = 58/204 (28.43%), Postives = 108/204 (52.94%), Query Frame = 0 

Query: 1   MEFQILKSLLTLFFFVSITITTSVVASSSSITRPVKP--YIIKACKTTPYPRLCEISLTL 60
           ME ++      L  F+ + +  S +A  SS   P     +I+ +C+ T Y  LC   L  
Sbjct: 1   MEPKLTHLCYCLLLFLPL-LCQSTIAKPSSSPNPSSSINFIVSSCRVTRYQTLCVKCLAA 60

Query: 61  HASQTKKTQQEFCRATMASSLKTTRDATSIISKLSR-QKMSTYEAKVIRDCVDNLKDSTD 120
            A + ++ + +  +  +A +L   +  T  + KL++ +++   E   ++DCV+NL D  +
Sbjct: 61  FADKIRRNENQLAQTALAVTLVRVQSTTIYVGKLTKARRIKRREYLAVKDCVENLGDGLE 120

Query: 121 ELRRAIAAIKSLSRA-KDAN---FQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVK 180
            L +++  +K + R+ +D +   ++L +++TW+SAA TD  TC DG     G+ +   VK
Sbjct: 121 MLAQSMRELKQVGRSGRDRDEFLWRLSNVETWVSAALTDETTCLDGFD---GKVMDGVVK 180

Query: 181 QEVKKSVAVVVRHISNALSLLNTF 198
             +++ V  V R  SNAL+L+N F
Sbjct: 181 SAIRRRVVHVARVTSNALALVNRF 200

[Close]
BLAST of Carg08008 vs. Swiss-Prot
Match: sp|P17407|21KD_DAUCA (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.1e-19
Identity = 61/187 (32.62%), Postives = 104/187 (55.61%), Query Frame = 0 

Query: 17  SITITTSVVASSSSITRPVKPYIIKACKTTPYPRLCEISLTLHASQTKKTQQEFCRATMA 76
           ++  +  +V  +++   P   +I  +C  T YP +CE SL+ +A   +   QE     + 
Sbjct: 7   TLVFSALLVILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQ 66

Query: 77  SSLKTTRDATSIISKLSRQK-MSTYEAKVIRDCVDNLKDSTDELRRAIAAIKSLSRAK-- 136
            SL  T+ A + + +L++ K +   +   I DC++ ++DS D + R+   +K+LS AK  
Sbjct: 67  VSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGN 126

Query: 137 DANFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSVAVVVRHISNALSL 196
           D  F++ +++TW+SAA TD  TC DG    AG+ +  K+K+ V+  V  V R  SNAL+L
Sbjct: 127 DFTFRMSNVETWVSAALTDETTCMDGF---AGKGMDGKIKESVRAQVVAVARVTSNALAL 186

Query: 197 LNTFKYK 201
           +N F  K
Sbjct: 187 VNNFAAK 190

[Close]
BLAST of Carg08008 vs. Swiss-Prot
Match: sp|Q9SI72|PMEI9_ARATH (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 6.2e-15
Identity = 65/204 (31.86%), Postives = 113/204 (55.39%), Query Frame = 0 

Query: 6   LKSLLTLFFFVSITITTSVVASSSSITRPVKPYIIKACKTTPYPRLCEISLTLHASQTK- 65
           LK+ + L   +SITI  S  +S++        +I+ +C+TT YP LC  +L+ +A++ + 
Sbjct: 3   LKNTIFLVILLSITILQS--SSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRH 62

Query: 66  KTQQEFCRATMASSLKTTRDATSIISKLSRQ--KMSTYEAKVIRDCVDNLKDSTDELRRA 125
              Q+  +  +  SL   +  T  ++KL+++  K    E   I+DC++ L +S D L + 
Sbjct: 63  NNDQDLAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQ- 122

Query: 126 IAAIKSLSRA------KDANFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEV 185
             ++K L+RA      +D  +++ +++TW+SAA TD  TC DG S    R +  KVK+ +
Sbjct: 123 --SVKELARAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFS---ERAMGGKVKRLI 182

Query: 186 KKSVAVVVRHISNALSLLNTFKYK 201
           +  V  V +  SNAL+L+N F  K
Sbjct: 183 RYKVVHVAQVTSNALALVNQFAEK 198

[Close]
BLAST of Carg08008 vs. Swiss-Prot
Match: sp|Q9STY5|PMI11_ARATH (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 4.0e-14
Identity = 60/200 (30.00%), Postives = 103/200 (51.50%), Query Frame = 0 

Query: 11  TLFFFVSITITTSVVASSSSITRPVKP------YIIKACKTTPYPRLCEISLTLHASQTK 70
           TLF F+   ++T+++ +SSS  R          +I  +CK T YP +C  SLT +A+  +
Sbjct: 8   TLFLFL---LSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQ 67

Query: 71  KTQQEFCRATMASSLKTTRDATSIISKLSR-QKMSTYEAKVIRDCVDNLKDSTDELRRAI 130
            + +      +  ++   +     + +L R   +   E + ++DC++ + D+ D L  +I
Sbjct: 68  TSPRRLAETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSI 127

Query: 131 AAIKSLSRAKDAN---FQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 190
             +K    AK  +   F + + +TW SAA T+A TCSDG    AGR +  +VK  V+  +
Sbjct: 128 HEVKMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGF---AGRVMDGRVKNSVRARI 187

Query: 191 AVVVRHISNALSLLNTFKYK 201
             + R  SNAL+L+N F  K
Sbjct: 188 LNLGRGTSNALALINAFAKK 201

[Close]
BLAST of Carg08008 vs. Swiss-Prot
Match: sp|Q9SI74|PMI10_ARATH (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.4e-11
Identity = 49/163 (30.06%), Postives = 85/163 (52.15%), Query Frame = 0 

Query: 38  YIIKACKTTPYPRLCEISLTLHASQTKKTQQEFCRATMASSLKTTRDATSIISKLSR-QK 97
           YI  +C  T Y  +C  SL+ +AS  +   Q+     +  +L + + A+  +  +S    
Sbjct: 147 YIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKFVKNISHGGG 206

Query: 98  MSTYEAKVIRDCVDNLKDSTDELRRAIAAIKSLSRAKDANFQ--LHSIKTWMSAAETDAM 157
           ++  E   + DCV+ + DS   L+ +I  + S++    A F+  +  ++TW+SAA T+  
Sbjct: 207 LTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETWVSAALTNDD 266

Query: 158 TCSDGLSGGAGRKVRSKVKQEVKKSVAVVVRHISNALSLLNTF 198
           TC DG S      V++ VK  V++ V  V R  SNAL+L+N +
Sbjct: 267 TCMDGFS-----LVKTAVKDLVRRHVVEVARLTSNALALINMY 304

[Close]
BLAST of Carg08008 vs. Swiss-Prot
Match: sp|O22244|PMEI6_ARATH (Pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PMEI6 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.2e-10
Identity = 48/173 (27.75%), Postives = 86/173 (49.71%), Query Frame = 0 

Query: 27  SSSSITRPVKPYIIKACKTTPYPRLCEISLTLHASQTKKTQQEFCRATMASSLKTTRDAT 86
           +++ I      Y+  AC  T Y RLC  +L   A   +    ++ RA++A ++  T+   
Sbjct: 37  NTNDIVTQYSTYVRNACNVTRYNRLCVRTLWPFAIVARNNTSKWARASVAVTITDTKRVL 96

Query: 87  SIISKLSRQKMSTYEAKVIRDCVDNLKDSTDELRRAIAAIKSLSRAKDANFQLHSIKTWM 146
            ++ K  R  +   E   + DC +   DS D L +++A +++L+ A +   Q+  + TW+
Sbjct: 97  RLLLKTQRSAVGESERIALSDCRELFVDSLDNLYKSLAVLRTLN-ADEFQRQISDLATWL 156

Query: 147 SAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSVAVVVRHISNALSLLNTFKY 200
           SAA TD  TC DG    +    R++  + V++     +R  SNAL+LL    +
Sbjct: 157 SAALTDDDTCLDGFEETSS---RTRTVRMVRRKATKCMRLCSNALALLKKLAF 205

[Close]
BLAST of Carg08008 vs. TrEMBL
Match: tr|A0A1S3AXZ9|A0A1S3AXZ9_CUCME (21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103483989 PE=4 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 1.2e-55
Identity = 119/168 (70.83%), Postives = 138/168 (82.14%), Query Frame = 0 

Query: 33  RPVKPYIIKACKTTPYPRLCEISLTLHASQTKKTQQEFCRATMASSLKTTRDATSIISKL 92
           RPVKP+I KACK TPYPRLCE +L+L+ASQTK+ QQE CRA M SSLK  ++ATSIISKL
Sbjct: 35  RPVKPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKL 94

Query: 93  SRQKMSTYEAKVIRDCVDNLKDSTDELRRAIAAIKSLSRAKDANFQLHSIKTWMSAAETD 152
           SR+KMS YE +VI DC+D+LKDS DELRRA  AIKSLSR+KD +FQL+SIKTW SAA TD
Sbjct: 95  SRRKMSAYETEVIGDCIDDLKDSVDELRRASTAIKSLSRSKDVDFQLNSIKTWTSAALTD 154

Query: 153 AMTCSDGLSGGAGRKVRSKVKQEVKKSVAVVVRHISNALSLLNTFKYK 201
            +TC+DGLSGG+G KV  KVK+EVK     VVR ISNALSL+N F YK
Sbjct: 155 IITCTDGLSGGSGWKVNKKVKKEVKNCSINVVRQISNALSLINNFNYK 202

[Close]
BLAST of Carg08008 vs. TrEMBL
Match: tr|A0A0A0L671|A0A0A0L671_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G176250 PE=4 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 2.1e-55
Identity = 117/168 (69.64%), Postives = 139/168 (82.74%), Query Frame = 0 

Query: 33  RPVKPYIIKACKTTPYPRLCEISLTLHASQTKKTQQEFCRATMASSLKTTRDATSIISKL 92
           RPV+P+I KACK TPYPRLCE +L+L+ASQTK+ QQE CRA M SSLK  ++ATSIISKL
Sbjct: 36  RPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKL 95

Query: 93  SRQKMSTYEAKVIRDCVDNLKDSTDELRRAIAAIKSLSRAKDANFQLHSIKTWMSAAETD 152
           SR+KMS YEA+VI DC+DNLKDS DELRRA  AIKSLSR+KD +FQL+SIKTWMSAA+TD
Sbjct: 96  SRRKMSAYEAEVIGDCIDNLKDSVDELRRASTAIKSLSRSKDVDFQLNSIKTWMSAAQTD 155

Query: 153 AMTCSDGLSGGAGRKVRSKVKQEVKKSVAVVVRHISNALSLLNTFKYK 201
            +TC+DGLSGG+G KV   +K++VK     VVR ISNAL L+N F YK
Sbjct: 156 VITCTDGLSGGSGWKVSKMLKKDVKNCSINVVRQISNALFLINNFNYK 203

[Close]
BLAST of Carg08008 vs. TrEMBL
Match: tr|B9R9Q8|B9R9Q8_RICCO (21 kDa protein, putative OS=Ricinus communis OX=3988 GN=RCOM_1500020 PE=4 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 8.4e-25
Identity = 72/192 (37.50%), Postives = 116/192 (60.42%), Query Frame = 0 

Query: 9   LLTLFFFVSITITTSVVASSSSITRPVKPYIIKACKTTPYPRLCEISLTLHASQTKKTQQ 68
           L+T   F+S T  +  + ++ +     K Y+  AC +T YP++C  +L+ + S  +    
Sbjct: 18  LITFLVFISNTQNSLAITTTKTSKTTYKNYLKTACNSTTYPKICYNTLSPYTSTIQTNDL 77

Query: 69  EFCRATMASSLKTTRDATSIISKLSRQK-MSTYEAKVIRDCVDNLKDSTDELRRAIAAIK 128
           + C A +  +LK   + ++++  LS+QK +S  E  VI+DC   ++DS DEL++++ A+K
Sbjct: 78  KLCNAALTITLKAASNTSAMVKSLSKQKGLSKGEVAVIKDCQYEIEDSVDELKQSLKALK 137

Query: 129 SLSRAKDANFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSVAVVVRHI 188
           +L  + D  FQ+ +IKTW+SAA TD  TC+DG     G KV SKVK ++KKS+  V R  
Sbjct: 138 NLKGSADMEFQIDNIKTWISAAITDENTCTDGFE---GMKVSSKVKSKIKKSIVNVNRLT 197

Query: 189 SNALSLLNTFKY 200
           SNAL+L+N   Y
Sbjct: 198 SNALALINKLSY 206

[Close]
BLAST of Carg08008 vs. TrEMBL
Match: tr|A0A2I4HWD8|A0A2I4HWD8_9ROSI (21 kDa protein-like OS=Juglans regia OX=51240 GN=LOC109022115 PE=4 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.4e-24
Identity = 77/202 (38.12%), Postives = 126/202 (62.38%), Query Frame = 0 

Query: 1   MEFQILKSLLTLFFFVSITITTSVVASSSSITRPVKPYIIKACKTTPYPRLCEISLTLHA 60
           + + +   LLTL F     +T   V ++++ ++  + YII +C +T YP+LC  SL+ +A
Sbjct: 12  ISYHVFMMLLTLLFM----LTLQAVLATTNTSQTYENYIITSCNSTTYPQLCYTSLSPYA 71

Query: 61  SQTKKTQQEFCRATMASSLKTTRDATSIISK-LSRQK--MSTYEAKVIRDCVDNLKDSTD 120
           S+ K   ++ C+ +++  L+  ++A+SI+SK L +QK  +++ E  VI+DC+ N+K+S D
Sbjct: 72  SKIKANPKKLCKTSLSVGLEAAQNASSIVSKQLIKQKGSLTSSETAVIKDCIVNIKNSID 131

Query: 121 ELRRAIAAIKSLSRAKDANFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVK 180
           ELR ++ A+  LS   D  FQ+ +IKTW+SAA TD  TC DG     G+++  KVK +V+
Sbjct: 132 ELRESLDAMDKLS-GWDKEFQMSNIKTWVSAALTDENTCLDGFE--EGQEISEKVKNKVR 191

Query: 181 KSVAVVVRHISNALSLLNTFKY 200
           K +  V R  SNAL L+N   Y
Sbjct: 192 KRILEVARLTSNALYLINNLNY 206

[Close]
BLAST of Carg08008 vs. TrEMBL
Match: tr|F6HAT8|F6HAT8_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_16s0022g00940 PE=4 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 5.5e-24
Identity = 78/195 (40.00%), Postives = 121/195 (62.05%), Query Frame = 0 

Query: 3   FQILKSL-LTLFFFVSITITTSVVASSSSITRPVKPYIIKACKTTPYPRLCEISLTLHAS 62
           F +L SL  TL  F SIT+T+S   S+ S+T   K +I  +C +T YP +C  SL+ +AS
Sbjct: 12  FHVLSSLFFTLLLFNSITLTSSANTSTISLTIS-KQFIKTSCNSTTYPGVCYKSLSSYAS 71

Query: 63  QTKKTQQEFCRATMASSLKTTRDATSIIS-KLSRQKMSTYEAKVIRDCVDNLKDSTDELR 122
           + + +  +     +  SLK  R+A+S I+  L R+ M+  E  +++DCV+N  D  DEL+
Sbjct: 72  KVQTSPLKLANVALTVSLKAARNASSTITLLLERKGMTRMETSIVKDCVENFGDCIDELQ 131

Query: 123 RAIAAIKSLSRAKDANFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 182
           +++   KSL   K+  FQ+ +IKTW+SAA TD  TCSDG     G+KV S ++Q++K  +
Sbjct: 132 QSLQEFKSLGGGKNVAFQMANIKTWVSAALTDEYTCSDGFE---GQKVSSSLQQQIKNYI 191

Query: 183 AVVVRHISNALSLLN 196
           + V +  SNAL+L+N
Sbjct: 192 SNVAKITSNALALIN 202

The following BLAST results are available for this feature:
BLAST of Carg08008 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022942851.11.5e-9798.5121 kDa protein-like [Cucurbita moschata][more]
XP_023542401.12.3e-9596.0221 kDa protein-like [Cucurbita pepo subsp. pepo][more]
XP_022987359.12.9e-9395.0221 kDa protein-like [Cucurbita maxima][more]
XP_022985512.16.0e-5967.35pectinesterase inhibitor 4-like [Cucurbita maxima][more]
XP_008439099.11.8e-5570.83PREDICTED: 21 kDa protein-like [Cucumis melo][more]
BLAST of Carg08008 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G62350.15.5e-2232.34Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G62770.13.5e-1631.86Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G14890.11.7e-1531.71Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT3G47380.12.2e-1530.00Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT4G12390.14.2e-1428.43pectin methylesterase inhibitor 1[more]
BLAST of Carg08008 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|P17407|21KD_DAUCA1.1e-1932.6221 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1[more]
sp|Q9SI72|PMEI9_ARATH6.2e-1531.86Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1[more]
sp|Q9STY5|PMI11_ARATH4.0e-1430.00Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1[more]
sp|Q9SI74|PMI10_ARATH2.4e-1130.06Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1[more]
sp|O22244|PMEI6_ARATH1.2e-1027.75Pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PMEI6 PE=2 SV=1[more]
BLAST of Carg08008 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S3AXZ9|A0A1S3AXZ9_CUCME1.2e-5570.8321 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103483989 PE=4 SV=1[more]
tr|A0A0A0L671|A0A0A0L671_CUCSA2.1e-5569.64Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G176250 PE=4 SV=1[more]
tr|B9R9Q8|B9R9Q8_RICCO8.4e-2537.5021 kDa protein, putative OS=Ricinus communis OX=3988 GN=RCOM_1500020 PE=4 SV=1[more]
tr|A0A2I4HWD8|A0A2I4HWD8_9ROSI1.4e-2438.1221 kDa protein-like OS=Juglans regia OX=51240 GN=LOC109022115 PE=4 SV=1[more]
tr|F6HAT8|F6HAT8_VITVI5.5e-2440.00Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_16s0022g00940 PE=4 SV=... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR035513Invertase/methylesterase_inhib
IPR006501Pectinesterase_inhib_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08008-RACarg08008-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 112..132
NoneNo IPR availablePANTHERPTHR31080:SF15INVERTASEcoord: 9..197
NoneNo IPR availablePANTHERPTHR31080FAMILY NOT NAMEDcoord: 9..197
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 42..197
e-value: 4.72974E-33
score: 116.769
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 34..193
e-value: 8.4E-36
score: 134.9
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 40..193
e-value: 6.6E-28
score: 97.9
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 11..196
e-value: 1.0E-29
score: 101.1
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3DG3DSA:1.20.140.40coord: 36..196
e-value: 1.3E-17
score: 66.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILYSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 38..197