Cucsa.251560 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.251560
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionPlant invertase/pectin methylesterase inhibitor
Locationscaffold02229 : 602908 .. 604310 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTCTTGAGAGAGAAATGGAATCTCAAATCCTAAAATCCTCACTAACCCTTTTGATCTTCTTCTTCATTATTTTAACTACATTCACCCCCTCCGCCGTGTCTTCTTCTTCCTCCACTGTCCGGCCGGTACAACCACACATCAGAAAGGCGTGCAAGCCAACGCCATATCCTCGCCTTTGCGAGACTGCACTTTCCTTATACGCTTCACAAACCAAAAGAAACCAGCAAGAACTATGCAGGGCGGCCATGGTTTCTTCTCTCAAAGCTGCACAAAACGCCACCTCCATCATCTCCAAGCTCTCCCGTCGGAAGATGTCAGCCTACGAAGCCGAAGTCATCGGTGATTGTATCGATAATCTCAAGGACTCTGTTGACGAGCTCCGTCGGGCATCAACTGCCATCAAAAGCTTGAGTCGTTCTAAGGATGTGGATTTTCAGGTTTTCAATAACTACTTAATAATCTCATATATAACGTCTTAATCTCGTTTTCACTTTAGGTTTAAAACAAAATCATTCTTCATTCTCAATTTTTATGATATTAACCATTCATTTTCTAAACTTCATGCCACTATATACTGTATTTCATATTATTTTAAATTTATAATATAGTTTAGTACCTTAAACTTTAAAAACCAACGATTTGTTTCCATATATATATAATGTCACAATTAGTAATATGTAATGACATATATTTTTAGCTTATAAATAAATTTATTTTTTTATTAATTTATCATCAACATTGCAAGAAGTTTTATTAAAGCATATTAATAGTGCAAATCTTAATCTATATTTAAGATTTATATTTTATTAAAGTTGAAAAAAGTAAAATCGTTGTAAATTTGAAAATATAGAAATTAATTTGTTCTTTTAACGAAAGTTGGGCTTCTAAATGTTATTTTTCTACATAATGTATATGAGTGAAATTTAAAGTTATGGTTAAAAAATAATAATAATAAATCGTAATGGATAGACATCGACATGTATTCTTTAAAAAAAAACCATGAAAATCAAAATAACTTTTAAAAAAATTACAAATAAGTATTATATGTATTTCACAGCTTTCAAATAAAGTTAAGAAGTTTTACTGTTATTAAGACTATGTTTGCATAATATGATATGGTCTAGCTCATTCAAATAACGTTACTTTTTAATCCTAAAACTAGGTTAAGTAACGTTAACATACAATCTCTTATTCATATATTCGTAATGTGTAGTTGAATAGTATAAAGACGTGGACGAGTGCAGCACAGACCGACGTTATAACATGCACGGATGGATTGAGCGGGGGCTCCGGGTGGAAGGTGAGCAAGATGTTGAAGAAAGAGGTGAAGAATTGTAGCATAAATGTGGTAAGGCAGATTAGTAATGCCTTATTTCTCATCAACAACTTTAACTACAAATGA

mRNA sequence

ATTCTTGAGAGAGAAATGGAATCTCAAATCCTAAAATCCTCACTAACCCTTTTGATCTTCTTCTTCATTATTTTAACTACATTCACCCCCTCCGCCGTGTCTTCTTCTTCCTCCACTGTCCGGCCGGTACAACCACACATCAGAAAGGCGTGCAAGCCAACGCCATATCCTCGCCTTTGCGAGACTGCACTTTCCTTATACGCTTCACAAACCAAAAGAAACCAGCAAGAACTATGCAGGGCGGCCATGGTTTCTTCTCTCAAAGCTGCACAAAACGCCACCTCCATCATCTCCAAGCTCTCCCGTCGGAAGATGTCAGCCTACGAAGCCGAAGTCATCGGTGATTGTATCGATAATCTCAAGGACTCTGTTGACGAGCTCCGTCGGGCATCAACTGCCATCAAAAGCTTGAGTCGTTCTAAGGATGTGGATTTTCAGTTGAATAGTATAAAGACGTGGACGAGTGCAGCACAGACCGACGTTATAACATGCACGGATGGATTGAGCGGGGGCTCCGGGTGGAAGGTGAGCAAGATGTTGAAGAAAGAGGTGAAGAATTGTAGCATAAATGTGGTAAGGCAGATTAGTAATGCCTTATTTCTCATCAACAACTTTAACTACAAATGA

Coding sequence (CDS)

ATGGAATCTCAAATCCTAAAATCCTCACTAACCCTTTTGATCTTCTTCTTCATTATTTTAACTACATTCACCCCCTCCGCCGTGTCTTCTTCTTCCTCCACTGTCCGGCCGGTACAACCACACATCAGAAAGGCGTGCAAGCCAACGCCATATCCTCGCCTTTGCGAGACTGCACTTTCCTTATACGCTTCACAAACCAAAAGAAACCAGCAAGAACTATGCAGGGCGGCCATGGTTTCTTCTCTCAAAGCTGCACAAAACGCCACCTCCATCATCTCCAAGCTCTCCCGTCGGAAGATGTCAGCCTACGAAGCCGAAGTCATCGGTGATTGTATCGATAATCTCAAGGACTCTGTTGACGAGCTCCGTCGGGCATCAACTGCCATCAAAAGCTTGAGTCGTTCTAAGGATGTGGATTTTCAGTTGAATAGTATAAAGACGTGGACGAGTGCAGCACAGACCGACGTTATAACATGCACGGATGGATTGAGCGGGGGCTCCGGGTGGAAGGTGAGCAAGATGTTGAAGAAAGAGGTGAAGAATTGTAGCATAAATGTGGTAAGGCAGATTAGTAATGCCTTATTTCTCATCAACAACTTTAACTACAAATGA

Protein sequence

MESQILKSSLTLLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSVDELRRASTAIKSLSRSKDVDFQLNSIKTWTSAAQTDVITCTDGLSGGSGWKVSKMLKKEVKNCSINVVRQISNALFLINNFNYK*
Homology
[Close]
BLAST of Cucsa.251560 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.5e-24
Identity = 65/185 (35.14%), Postives = 107/185 (57.84%), Query Frame = 1 

Query: 23  FTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSL 82
           F+   V  ++++  P    I+ +C  T YP +CE +LS YA   + N QEL   A+  SL
Sbjct: 10  FSALLVILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQVSL 69

Query: 83  KAAQNATSIISKLSRRK-MSAYEAEVIGDCIDNLKDSVDELRRASTAIKSLSRSK--DVD 142
              Q A + + +L++ K + A +   I DC++ ++DS+D + R+   +K+LS +K  D  
Sbjct: 70  TRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGNDFT 129

Query: 143 FQLNSIKTWTSAAQTDVITCTDGLSG-GSGWKVSKMLKKEVKNCSINVVRQISNALFLIN 202
           F++++++TW SAA TD  TC DG +G G   K+ + ++ +V    + V R  SNAL L+N
Sbjct: 130 FRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQV----VAVARVTSNALALVN 189

Query: 203 NFNYK 204
           NF  K
Sbjct: 190 NFAAK 190

[Close]
BLAST of Cucsa.251560 vs. Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 68.9 bits (167), Expect = 7.1e-11
Identity = 44/174 (25.29%), Postives = 87/174 (50.00%), Query Frame = 1 

Query: 28  VSSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQN 87
           +++ SST   +   ++  C  T YP LC +A++    +   +Q+E+  A++  + KA ++
Sbjct: 51  ITTLSSTSHAI---LKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKH 110

Query: 88  ATSIISKL--SRRKMSAYEAEVIGDCIDNLKDSVDELRRASTAIKSLSRSKDVDFQLNSI 147
               + KL   R+ ++  E   + DC++ + +++DEL  A   +    + K +    + +
Sbjct: 111 NYFAVKKLIAKRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDL 170

Query: 148 KTWTSAAQTDVITCTDGLSGGSGWKVSKMLKKEVKNCSINVVRQISNALFLINN 200
           KT  S+A T+  TC DG S        + ++K +    ++V    SNAL +I N
Sbjct: 171 KTLISSAITNQGTCLDGFSYDD---ADRKVRKALLKGQVHVEHMCSNALAMIKN 218

[Close]
BLAST of Cucsa.251560 vs. Swiss-Prot
Match: PME26_ARATH (Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana GN=PME26 PE=2 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.8e-06
Identity = 49/191 (25.65%), Postives = 81/191 (42.41%), Query Frame = 1 

Query: 20  LTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMV 79
           L   +P A   S   + P    +R  C  T YP  C +++S   S    + + L R ++ 
Sbjct: 250 LLNSSPHATPISVPKLTPAAS-LRNVCSVTRYPASCVSSISKLPSSNTTDPEALFRLSLQ 309

Query: 80  SSLKAAQNATSIISKLS------RRKMS-AYEAEVIGDCIDNLKDSVDELRRASTAIKSL 139
             +    +   +  KL+      R K S +   +V  D ID + D++  +       K L
Sbjct: 310 VVINELNSIAGLPKKLAEETDDERLKSSLSVCGDVFNDAIDIVNDTISTMEEVGDGKKIL 369

Query: 140 SRSKDVDFQLNSIKTWTSAAQTDVITCTDGLSGGSGWK---VSKMLKKEVKNCSINVVRQ 199
             S      ++ I+TW SAA TD  TC D L   S  K    +  +  ++K+  +N  + 
Sbjct: 370 KSST-----IDEIQTWLSAAVTDHDTCLDALDELSQNKTEYANSPISLKLKSAMVNSRKF 429

Query: 200 ISNALFLINNF 201
            SN+L +I  F
Sbjct: 430 TSNSLAIIAKF 434

[Close]
BLAST of Cucsa.251560 vs. Swiss-Prot
Match: PME1_ARATH (Pectinesterase 1 OS=Arabidopsis thaliana GN=PME1 PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.8e-06
Identity = 35/168 (20.83%), Postives = 74/168 (44.05%), Query Frame = 1 

Query: 4   QILKSSLTLLIFFFIILTTFTPSAVSSSSSTVRPVQP-------HIRKACKPTPYPRLCE 63
           ++L  S+++++   +I+     + V  + +   P  P        ++  C  T +P  C 
Sbjct: 28  RLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPPELTPSTSLKAICSVTRFPESCI 87

Query: 64  TALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLK 123
           +++S   S    + + L + ++   +    + + +  KLS+          +  C D ++
Sbjct: 88  SSISKLPSSNTTDPETLFKLSLKVIIDELDSISDLPEKLSKETEDERIKSALRVCGDLIE 147

Query: 124 DSVDELRRASTAIKSLSRSKDVDF-QLNSIKTWTSAAQTDVITCTDGL 164
           D++D L    +AI    + K +   ++  +KTW SA  TD  TC D L
Sbjct: 148 DALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVTDHETCFDSL 195

[Close]
BLAST of Cucsa.251560 vs. TrEMBL
Match: A0A0A0L671_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176250 PE=4 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 7.0e-106
Identity = 201/203 (99.01%), Postives = 202/203 (99.51%), Query Frame = 1 

Query: 1   MESQILKSSLTLLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALS 60
           MESQILKSSLTLLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALS
Sbjct: 1   MESQILKSSLTLLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALS 60

Query: 61  LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSVD 120
           LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSVD
Sbjct: 61  LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSVD 120

Query: 121 ELRRASTAIKSLSRSKDVDFQLNSIKTWTSAAQTDVITCTDGLSGGSGWKVSKMLKKEVK 180
           ELRRASTAIKSLSRSKDVDFQLNSIKTW SAAQTDVITCTDGLSGGSGWKVSKMLKK+VK
Sbjct: 121 ELRRASTAIKSLSRSKDVDFQLNSIKTWMSAAQTDVITCTDGLSGGSGWKVSKMLKKDVK 180

Query: 181 NCSINVVRQISNALFLINNFNYK 204
           NCSINVVRQISNALFLINNFNYK
Sbjct: 181 NCSINVVRQISNALFLINNFNYK 203

[Close]
BLAST of Cucsa.251560 vs. TrEMBL
Match: B9R9Q8_RICCO (21 kDa protein, putative OS=Ricinus communis GN=RCOM_1500020 PE=4 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 7.9e-33
Identity = 80/196 (40.82%), Postives = 122/196 (62.24%), Query Frame = 1 

Query: 8   SSLTLLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTK 67
           S+L L+ F   I  T    A++++ ++    + +++ AC  T YP++C   LS Y S  +
Sbjct: 14  STLFLITFLVFISNTQNSLAITTTKTSKTTYKNYLKTACNSTTYPKICYNTLSPYTSTIQ 73

Query: 68  RNQQELCRAAMVSSLKAAQNATSIISKLSRRK-MSAYEAEVIGDCIDNLKDSVDELRRAS 127
            N  +LC AA+  +LKAA N ++++  LS++K +S  E  VI DC   ++DSVDEL+++ 
Sbjct: 74  TNDLKLCNAALTITLKAASNTSAMVKSLSKQKGLSKGEVAVIKDCQYEIEDSVDELKQSL 133

Query: 128 TAIKSLSRSKDVDFQLNSIKTWTSAAQTDVITCTDGLSGGSGWKVSKMLKKEVKNCSINV 187
            A+K+L  S D++FQ+++IKTW SAA TD  TCTDG     G KVS  +K ++K   +NV
Sbjct: 134 KALKNLKGSADMEFQIDNIKTWISAAITDENTCTDGF---EGMKVSSKVKSKIKKSIVNV 193

Query: 188 VRQISNALFLINNFNY 203
            R  SNAL LIN  +Y
Sbjct: 194 NRLTSNALALINKLSY 206

[Close]
BLAST of Cucsa.251560 vs. TrEMBL
Match: M5X0K1_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011478mg PE=4 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.1e-30
Identity = 84/195 (43.08%), Postives = 122/195 (62.56%), Query Frame = 1 

Query: 10  LTLLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRN 69
           + LLIF   + TT    A SSSS T +    +++ AC  T YP +C  +LS YAS+ K +
Sbjct: 23  ILLLIFMPNMHTTL---ATSSSSQTYKT---YVKTACNTTTYPLICYKSLSSYASKVKSD 82

Query: 70  QQELCRAAMVSSLKAAQNATSIISKLSRRK-MSAYEAEVIGDCIDNLKDSVDELRRASTA 129
            Q+LC  A+  +LKAA+NA+S++SKL +   ++  E  V+ DCI+N+KDS+DEL+++ ++
Sbjct: 83  PQKLCTYALSVTLKAAKNASSVVSKLYKNTGLTPSEKGVVKDCIENIKDSIDELKQSVSS 142

Query: 130 IKSLSRS-KDVDFQLNSIKTWTSAAQTDVITCTDGLSGGSGWKVSKMLKKEVKNCSINVV 189
           + +L  S  DV  QL+ IKTW SA  TD  TCTDG     G KVS  +K  ++N  +N  
Sbjct: 143 MSNLGVSGSDVQLQLDDIKTWVSAVITDDATCTDGF---DGVKVSAAVKTAIRNSIVNAA 202

Query: 190 RQISNALFLINNFNY 203
           R  SNAL LI++  Y
Sbjct: 203 RLASNALSLIDSLIY 208

[Close]
BLAST of Cucsa.251560 vs. TrEMBL
Match: F6HAT8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0022g00940 PE=4 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.1e-29
Identity = 80/192 (41.67%), Postives = 119/192 (61.98%), Query Frame = 1 

Query: 11  TLLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQ 70
           TLL+F  I LT    S+ ++S+ ++   +  I+ +C  T YP +C  +LS YAS+ + + 
Sbjct: 21  TLLLFNSITLT----SSANTSTISLTISKQFIKTSCNSTTYPGVCYKSLSSYASKVQTSP 80

Query: 71  QELCRAAMVSSLKAAQNATSIISKLSRRK-MSAYEAEVIGDCIDNLKDSVDELRRASTAI 130
            +L   A+  SLKAA+NA+S I+ L  RK M+  E  ++ DC++N  D +DEL+++    
Sbjct: 81  LKLANVALTVSLKAARNASSTITLLLERKGMTRMETSIVKDCVENFGDCIDELQQSLQEF 140

Query: 131 KSLSRSKDVDFQLNSIKTWTSAAQTDVITCTDGLSGGSGWKVSKMLKKEVKNCSINVVRQ 190
           KSL   K+V FQ+ +IKTW SAA TD  TC+DG     G KVS  L++++KN   NV + 
Sbjct: 141 KSLGGGKNVAFQMANIKTWVSAALTDEYTCSDGF---EGQKVSSSLQQQIKNYISNVAKI 200

Query: 191 ISNALFLINNFN 202
            SNAL LINN +
Sbjct: 201 TSNALALINNLS 205

[Close]
BLAST of Cucsa.251560 vs. TrEMBL
Match: A5BT23_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_005236 PE=4 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.1e-29
Identity = 80/192 (41.67%), Postives = 119/192 (61.98%), Query Frame = 1 

Query: 11  TLLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQ 70
           TLL+F  I LT    S+ ++S+ ++   +  I+ +C  T YP +C  +LS YAS+ + + 
Sbjct: 21  TLLLFNSITLT----SSANTSTISLTISKQFIKTSCNSTTYPGVCYKSLSSYASKVQTSP 80

Query: 71  QELCRAAMVSSLKAAQNATSIISKLSRRK-MSAYEAEVIGDCIDNLKDSVDELRRASTAI 130
            +L   A+  SLKAA+NA+S I+ L  RK M+  E  ++ DC++N  D +DEL+++    
Sbjct: 81  LKLANVALTVSLKAARNASSTITLLLERKGMTRMETSIVKDCVENFGDCIDELQQSLQEF 140

Query: 131 KSLSRSKDVDFQLNSIKTWTSAAQTDVITCTDGLSGGSGWKVSKMLKKEVKNCSINVVRQ 190
           KSL   K+V FQ+ +IKTW SAA TD  TC+DG     G KVS  L++++KN   NV + 
Sbjct: 141 KSLGGGKNVAFQMANIKTWVSAALTDEYTCSDGF---EGQKVSSSLQQQIKNYISNVAKI 200

Query: 191 ISNALFLINNFN 202
            SNAL LINN +
Sbjct: 201 TSNALALINNLS 205

[Close]
BLAST of Cucsa.251560 vs. TAIR10
Match: AT4G25250.1 (AT4G25250.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 114.4 bits (285), Expect = 8.3e-26
Identity = 64/195 (32.82%), Postives = 112/195 (57.44%), Query Frame = 1 

Query: 9   SLTLLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKR 68
           SLTL++F   I ++  P   ++   T +    +++ AC  T YP +C   LS Y+S  K 
Sbjct: 8   SLTLMVF---INSSNFPKTAATPPGTYQNHTTYVKTACNSTTYPTMCYNCLSSYSSTIKS 67

Query: 69  NQQELCRAAMVSSLKAAQNATSIISKLSRRKMSA--YEAEVIGDCIDNLKDSVDELRRAS 128
           +  +LC  ++  ++K+A+NAT ++S L ++  +A  +E  ++ DC+D +KD++DEL++A 
Sbjct: 68  DPIKLCTTSLNLNVKSAKNATLVVSNLLQKAKAAKSHEVSILKDCVDEMKDTIDELKQAV 127

Query: 129 TAIKSL-SRSKDVDFQLNSIKTWTSAAQTDVITCTDGLSGGSGWKVSKMLKKEVKNCSIN 188
             +K +    K  +  L ++KTW S+A TD  TCTDG   G   +V+   KK+VK     
Sbjct: 128 AEMKYVRGGGKTTEEHLKNVKTWVSSALTDEGTCTDGFEEG---RVNVETKKKVKKAISE 187

Query: 189 VVRQISNALFLINNF 201
           + +  SN L L+ ++
Sbjct: 188 LSKTTSNTLALLTHY 196

[Close]
BLAST of Cucsa.251560 vs. TAIR10
Match: AT5G51520.1 (AT5G51520.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 110.2 bits (274), Expect = 1.6e-24
Identity = 69/209 (33.01%), Postives = 110/209 (52.63%), Query Frame = 1 

Query: 3   SQILKSSLTLLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALSLY 62
           SQ+L S   + +FF    +T       S+SS  +  +  ++ AC  T YP  C  +LS Y
Sbjct: 2   SQVLYSLTIVFVFF---ASTNIQKTSGSASSYSQNHKTFVKTACNSTTYPDKCYKSLSSY 61

Query: 63  ASQTKRNQQELCRAAMVSSLKAAQNATSIISKL---------SRRKMSAYEAEVIGDCID 122
           +S  K +  +LC  A+  ++K+A+ ATS++SKL          R+     EA ++ DC++
Sbjct: 62  SSNIKSDPIKLCTTALNLNVKSAKEATSVVSKLLKMSQKSTAGRKGKMLPEALILKDCLE 121

Query: 123 NLKDSVDELRRASTAIKSLSRSKDVDFQLNSIKTWTSAAQTDVITCTDGLSGGSGWKVSK 182
            +KD++ EL++A T +K+L     +   + +++TW S+A TD  TCTDG       KV+K
Sbjct: 122 EMKDTIIELKQAITEMKNLQDGGSMAEHITNVRTWVSSALTDEGTCTDGF---EEVKVNK 181

Query: 183 MLKKEVKNCSINVVRQISNALFLINNFNY 203
             KK+V      +    SN L LI N  Y
Sbjct: 182 ETKKKVNKVVEELATTTSNTLALITNLRY 204

[Close]
BLAST of Cucsa.251560 vs. TAIR10
Match: AT5G62350.1 (AT5G62350.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 110.2 bits (274), Expect = 1.6e-24
Identity = 64/193 (33.16%), Postives = 105/193 (54.40%), Query Frame = 1 

Query: 12  LLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQ 71
           LL   +++    T +  +S +   +     I+ +CK T YP LC  +LS+YA+  + + +
Sbjct: 9   LLSISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYANDIQTSPK 68

Query: 72  ELCRAAMVSSLKAAQNATSIISKLSRRK-MSAYEAEVIGDCIDNLKDSVDELRRASTAIK 131
            L   A+  +L  AQ+    +S+L+R K +   E E I DC++ + D+VD L ++   +K
Sbjct: 69  RLAETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRLTKSVQELK 128

Query: 132 SLSRSKDVD---FQLNSIKTWTSAAQTDVITCTDGLSGGSGWKVSKMLKKEVKNCSINVV 191
               +KD D   + +++ +TWTSAA TD  TC+DG SG     +   +K  V+   +NV 
Sbjct: 129 LCGSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGR---VMDGRIKNSVRARIMNVG 188

Query: 192 RQISNALFLINNF 201
            + SNAL LIN F
Sbjct: 189 HETSNALSLINAF 198

[Close]
BLAST of Cucsa.251560 vs. TAIR10
Match: AT1G62770.1 (AT1G62770.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 102.1 bits (253), Expect = 4.3e-22
Identity = 69/199 (34.67%), Postives = 109/199 (54.77%), Query Frame = 1 

Query: 16  FFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTK-RNQQELC 75
           F +IL + T    SS++         I  +C+ T YP LC   LS YA++ +  N Q+L 
Sbjct: 8   FLVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLA 67

Query: 76  RAAMVSSLKAAQNATSIISKLSRR----KMSAYEA-----EVIGDCIDNLKDSVDELRRA 135
           + A++ SL  A++ T  ++KL++     K   Y A     EV+G+ +D L  SV EL RA
Sbjct: 68  QTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELARA 127

Query: 136 STAIKSLSRSKDVDFQLNSIKTWTSAAQTDVITCTDGLS-GGSGWKVSKMLKKEVKNCSI 195
             A+     S+D  +++++++TW SAA TD  TC DG S    G KV ++++ +V    +
Sbjct: 128 GHAV----ASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKV----V 187

Query: 196 NVVRQISNALFLINNFNYK 204
           +V +  SNAL L+N F  K
Sbjct: 188 HVAQVTSNALALVNQFAEK 198

[Close]
BLAST of Cucsa.251560 vs. TAIR10
Match: AT4G12390.1 (AT4G12390.1 pectin methylesterase inhibitor 1)

HSP 1 Score: 99.4 bits (246), Expect = 2.8e-21
Identity = 63/207 (30.43%), Postives = 113/207 (54.59%), Query Frame = 1 

Query: 1   MESQILKSSLTLLIFFFIIL--TTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETA 60
           ME ++      LL+F  ++   T   PS+  + SS++      I  +C+ T Y  LC   
Sbjct: 1   MEPKLTHLCYCLLLFLPLLCQSTIAKPSSSPNPSSSIN----FIVSSCRVTRYQTLCVKC 60

Query: 61  LSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSR-RKMSAYEAEVIGDCIDNLKD 120
           L+ +A + +RN+ +L + A+  +L   Q+ T  + KL++ R++   E   + DC++NL D
Sbjct: 61  LAAFADKIRRNENQLAQTALAVTLVRVQSTTIYVGKLTKARRIKRREYLAVKDCVENLGD 120

Query: 121 SVDELRRASTAIKSLSRS-KDVD---FQLNSIKTWTSAAQTDVITCTDGLSGGSGWKVSK 180
            ++ L ++   +K + RS +D D   ++L++++TW SAA TD  TC DG  G     +  
Sbjct: 121 GLEMLAQSMRELKQVGRSGRDRDEFLWRLSNVETWVSAALTDETTCLDGFDGK---VMDG 180

Query: 181 MLKKEVKNCSINVVRQISNALFLINNF 201
           ++K  ++   ++V R  SNAL L+N F
Sbjct: 181 VVKSAIRRRVVHVARVTSNALALVNRF 200

[Close]
BLAST of Cucsa.251560 vs. NCBI nr
Match: gi|449460810|ref|XP_004148137.1| (PREDICTED: 21 kDa protein-like [Cucumis sativus])

HSP 1 Score: 391.3 bits (1004), Expect = 1.0e-105
Identity = 201/203 (99.01%), Postives = 202/203 (99.51%), Query Frame = 1 

Query: 1   MESQILKSSLTLLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALS 60
           MESQILKSSLTLLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALS
Sbjct: 1   MESQILKSSLTLLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALS 60

Query: 61  LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSVD 120
           LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSVD
Sbjct: 61  LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSVD 120

Query: 121 ELRRASTAIKSLSRSKDVDFQLNSIKTWTSAAQTDVITCTDGLSGGSGWKVSKMLKKEVK 180
           ELRRASTAIKSLSRSKDVDFQLNSIKTW SAAQTDVITCTDGLSGGSGWKVSKMLKK+VK
Sbjct: 121 ELRRASTAIKSLSRSKDVDFQLNSIKTWMSAAQTDVITCTDGLSGGSGWKVSKMLKKDVK 180

Query: 181 NCSINVVRQISNALFLINNFNYK 204
           NCSINVVRQISNALFLINNFNYK
Sbjct: 181 NCSINVVRQISNALFLINNFNYK 203

[Close]
BLAST of Cucsa.251560 vs. NCBI nr
Match: gi|659077238|ref|XP_008439099.1| (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 370.2 bits (949), Expect = 2.4e-99
Identity = 191/203 (94.09%), Postives = 197/203 (97.04%), Query Frame = 1 

Query: 1   MESQILKSSLTLLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALS 60
           MESQILKSSLTL  FFFIILTTFTPSAV+SSSSTVRPV+PHIRKACKPTPYPRLCETALS
Sbjct: 1   MESQILKSSLTLF-FFFIILTTFTPSAVASSSSTVRPVKPHIRKACKPTPYPRLCETALS 60

Query: 61  LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSVD 120
           LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYE EVIGDCID+LKDSVD
Sbjct: 61  LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYETEVIGDCIDDLKDSVD 120

Query: 121 ELRRASTAIKSLSRSKDVDFQLNSIKTWTSAAQTDVITCTDGLSGGSGWKVSKMLKKEVK 180
           ELRRASTAIKSLSRSKDVDFQLNSIKTWTSAA TD+ITCTDGLSGGSGWKV+K +KKEVK
Sbjct: 121 ELRRASTAIKSLSRSKDVDFQLNSIKTWTSAALTDIITCTDGLSGGSGWKVNKKVKKEVK 180

Query: 181 NCSINVVRQISNALFLINNFNYK 204
           NCSINVVRQISNAL LINNFNYK
Sbjct: 181 NCSINVVRQISNALSLINNFNYK 202

[Close]
BLAST of Cucsa.251560 vs. NCBI nr
Match: gi|255539737|ref|XP_002510933.1| (PREDICTED: 21 kDa protein [Ricinus communis])

HSP 1 Score: 148.7 bits (374), Expect = 1.1e-32
Identity = 80/196 (40.82%), Postives = 122/196 (62.24%), Query Frame = 1 

Query: 8   SSLTLLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTK 67
           S+L L+ F   I  T    A++++ ++    + +++ AC  T YP++C   LS Y S  +
Sbjct: 14  STLFLITFLVFISNTQNSLAITTTKTSKTTYKNYLKTACNSTTYPKICYNTLSPYTSTIQ 73

Query: 68  RNQQELCRAAMVSSLKAAQNATSIISKLSRRK-MSAYEAEVIGDCIDNLKDSVDELRRAS 127
            N  +LC AA+  +LKAA N ++++  LS++K +S  E  VI DC   ++DSVDEL+++ 
Sbjct: 74  TNDLKLCNAALTITLKAASNTSAMVKSLSKQKGLSKGEVAVIKDCQYEIEDSVDELKQSL 133

Query: 128 TAIKSLSRSKDVDFQLNSIKTWTSAAQTDVITCTDGLSGGSGWKVSKMLKKEVKNCSINV 187
            A+K+L  S D++FQ+++IKTW SAA TD  TCTDG     G KVS  +K ++K   +NV
Sbjct: 134 KALKNLKGSADMEFQIDNIKTWISAAITDENTCTDGF---EGMKVSSKVKSKIKKSIVNV 193

Query: 188 VRQISNALFLINNFNY 203
            R  SNAL LIN  +Y
Sbjct: 194 NRLTSNALALINKLSY 206

[Close]
BLAST of Cucsa.251560 vs. NCBI nr
Match: gi|470146900|ref|XP_004309057.1| (PREDICTED: 21 kDa protein-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 147.1 bits (370), Expect = 3.3e-32
Identity = 85/192 (44.27%), Postives = 124/192 (64.58%), Query Frame = 1 

Query: 12  LLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQ 71
           LL+F   I TT   +A SSSS T +    +++ AC  T YP++C  +LS YA+  K N Q
Sbjct: 10  LLLFILFINTT---TATSSSSRTYKT---YVKTACNSTTYPQICYKSLSSYATSIKSNPQ 69

Query: 72  ELCRAAMVSSLKAAQNATSIISKLSRRK-MSAYEAEVIGDCIDNLKDSVDELRRASTAIK 131
           +LC  A+  +LKAA+ + S +SKL+++K ++  EA V+G CI+ +KDSVDEL+++ TA+K
Sbjct: 70  KLCSYALSVTLKAAKTSASTVSKLAKKKGITKAEAGVVGACIETIKDSVDELKQSVTAMK 129

Query: 132 SLSRSKDVDFQLNSIKTWTSAAQTDVITCTDGLSGGSGWKVSKMLKKEVKNCSINVVRQI 191
           +L    D+ +QL++IKTW SA+ TD  TCTDG       KVS  +K  ++   + V R  
Sbjct: 130 NLG-GADMQYQLDNIKTWVSASLTDEDTCTDGF---DEQKVSTAVKNTIRKSILTVARLA 189

Query: 192 SNALFLINNFNY 203
           SNAL LI+N NY
Sbjct: 190 SNALSLIDNLNY 191

[Close]
BLAST of Cucsa.251560 vs. NCBI nr
Match: gi|645257369|ref|XP_008234383.1| (PREDICTED: 21 kDa protein-like [Prunus mume])

HSP 1 Score: 141.4 bits (355), Expect = 1.8e-30
Identity = 85/195 (43.59%), Postives = 121/195 (62.05%), Query Frame = 1 

Query: 10  LTLLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRN 69
           + LLIF   + TT    A SSSS T +    +++ AC  T YP +C  +LS YAS+ K +
Sbjct: 23  ILLLIFMPNMHTTL---ATSSSSQTYKS---YVKTACNTTTYPLICYKSLSSYASKVKSD 82

Query: 70  QQELCRAAMVSSLKAAQNATSIISKLSRRK-MSAYEAEVIGDCIDNLKDSVDELRRASTA 129
             +LC  A+  +LKAA+NA+S++SKL +   ++  E  V+ DCIDN+KDS+DEL+++ ++
Sbjct: 83  PHKLCTYALSVTLKAAKNASSVVSKLYKNTGLTPSEKGVVKDCIDNIKDSIDELKQSVSS 142

Query: 130 IKSLSRS-KDVDFQLNSIKTWTSAAQTDVITCTDGLSGGSGWKVSKMLKKEVKNCSINVV 189
           + +L  S  DV  QL+ IKTW SA  TD  TCTDG     G KVS  +K  +KN  +N  
Sbjct: 143 MSNLGVSGSDVQLQLDDIKTWVSAVITDDATCTDGF---DGVKVSTAVKTAIKNSIVNAA 202

Query: 190 RQISNALFLINNFNY 203
           R  SNAL LI++  Y
Sbjct: 203 RLASNALSLIDSLIY 208

The following BLAST results are available for this feature:
BLAST of Cucsa.251560 vs. Swiss-Prot
Match NameE-valueIdentityDescription
21KD_DAUCA1.5e-2435.1421 kDa protein OS=Daucus carota PE=2 SV=1[more]
PME2_ARATH7.1e-1125.29Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2[more]
PME26_ARATH1.8e-0625.65Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana GN=P... [more]
PME1_ARATH1.8e-0620.83Pectinesterase 1 OS=Arabidopsis thaliana GN=PME1 PE=1 SV=1[more]
BLAST of Cucsa.251560 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L671_CUCSA7.0e-10699.01Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176250 PE=4 SV=1[more]
B9R9Q8_RICCO7.9e-3340.8221 kDa protein, putative OS=Ricinus communis GN=RCOM_1500020 PE=4 SV=1[more]
M5X0K1_PRUPE2.1e-3043.08Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011478mg PE=4 SV=1[more]
F6HAT8_VITVI1.1e-2941.67Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0022g00940 PE=4 SV=... [more]
A5BT23_VITVI1.1e-2941.67Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_005236 PE=4 SV=1[more]
BLAST of Cucsa.251560 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G25250.18.3e-2632.82 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G51520.11.6e-2433.01 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G62350.11.6e-2433.16 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G62770.14.3e-2234.67 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT4G12390.12.8e-2130.43 pectin methylesterase inhibitor 1[more]
BLAST of Cucsa.251560 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449460810|ref|XP_004148137.1|1.0e-10599.01PREDICTED: 21 kDa protein-like [Cucumis sativus][more]
gi|659077238|ref|XP_008439099.1|2.4e-9994.09PREDICTED: 21 kDa protein-like [Cucumis melo][more]
gi|255539737|ref|XP_002510933.1|1.1e-3240.82PREDICTED: 21 kDa protein [Ricinus communis][more]
gi|470146900|ref|XP_004309057.1|3.3e-3244.27PREDICTED: 21 kDa protein-like [Fragaria vesca subsp. vesca][more]
gi|645257369|ref|XP_008234383.1|1.8e-3043.59PREDICTED: 21 kDa protein-like [Prunus mume][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR006501Pectinesterase_inhib_dom
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.251560.1Cucsa.251560.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 41..200
score: 3.6
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 42..194
score: 7.4
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 37..196
score: 5.5
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 10..199
score: 5.5
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 40..200
score: 1.31
NoneNo IPR availablePANTHERPTHR31080FAMILY NOT NAMEDcoord: 1..199
score: 5.9
NoneNo IPR availablePANTHERPTHR31080:SF15SUBFAMILY NOT NAMEDcoord: 1..199
score: 5.9

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.251560Cla006265Watermelon (97103) v1cgywmB479
Cucsa.251560Csa3G176250Cucumber (Chinese Long) v2cgycuB366
Cucsa.251560CSPI03G16020Wild cucumber (PI 183967)cgycpiB383
Cucsa.251560CmaCh15G011540Cucurbita maxima (Rimu)cgycmaB0710
Cucsa.251560CmaCh04G003310Cucurbita maxima (Rimu)cgycmaB0722
Cucsa.251560MELO3C006820.2Melon (DHL92) v3.6.1cgymedB432
Cucsa.251560CsaV3_3G016220Cucumber (Chinese Long) v3cgycucB387
Cucsa.251560Carg08008Silver-seed gourdcarcgyB0437
Cucsa.251560Carg16733Silver-seed gourdcarcgyB0993
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.251560Cucurbita pepo (Zucchini)cgycpeB0673
Cucsa.251560Cucurbita pepo (Zucchini)cgycpeB0681
Cucsa.251560Cucurbita pepo (Zucchini)cgycpeB0686
Cucsa.251560Bottle gourd (USVL1VR-Ls)cgylsiB411
Cucsa.251560Bottle gourd (USVL1VR-Ls)cgylsiB416
Cucsa.251560Melon (DHL92) v3.6.1cgymedB422
Cucsa.251560Silver-seed gourdcarcgyB1042
Cucsa.251560Cucumber (Chinese Long) v3cgycucB392
Cucsa.251560Cucumber (Chinese Long) v3cgycucB395
Cucsa.251560Watermelon (97103) v2cgywmbB444
Cucsa.251560Watermelon (97103) v2cgywmbB452
Cucsa.251560Wax gourdcgywgoB522
Cucsa.251560Cucumber (Gy14) v1cgycgyB126
Cucsa.251560Cucurbita maxima (Rimu)cgycmaB0711
Cucsa.251560Cucurbita maxima (Rimu)cgycmaB0718
Cucsa.251560Cucurbita moschata (Rifu)cgycmoB0716
Cucsa.251560Cucurbita moschata (Rifu)cgycmoB0702
Cucsa.251560Cucurbita moschata (Rifu)cgycmoB0704
Cucsa.251560Cucurbita moschata (Rifu)cgycmoB0711
Cucsa.251560Wild cucumber (PI 183967)cgycpiB386
Cucsa.251560Wild cucumber (PI 183967)cgycpiB388
Cucsa.251560Cucumber (Chinese Long) v2cgycuB368
Cucsa.251560Cucumber (Chinese Long) v2cgycuB370
Cucsa.251560Melon (DHL92) v3.5.1cgymeB428
Cucsa.251560Melon (DHL92) v3.5.1cgymeB439
Cucsa.251560Watermelon (Charleston Gray)cgywcgB445
Cucsa.251560Watermelon (Charleston Gray)cgywcgB447
Cucsa.251560Watermelon (Charleston Gray)cgywcgB451
Cucsa.251560Watermelon (97103) v1cgywmB480