CmaCh04G003310 (gene) Cucurbita maxima (Rimu)

Overview
NameCmaCh04G003310
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionPlant invertase/pectin methylesterase inhibitor
LocationCma_Chr04 : 1631114 .. 1632204 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGATATGGAATTTCAAATCCTAAAATCGTTACTAACCCTTTTTTTTTTCGTTTCAATTACGTTCACCACCTCCGTCGTGGCTTCTTCTTCTTCCATTATCCGTCCAGTGAAACCATACATCAAAAAGGCTTGCAAAACGACGCCGTATCCACGCCTTTGCGAGATCTCCCTCACTTTACACGCTTCACAAACCAAAAAAACACAACAAGAGTTCTGTCGTGCGGCCATGGCTTCTTCTCTCAAAACTACACGAGATGCCACCTCCATAATCTCCAAGTTATCTCGCCAAAAGATGTCTACCTATGAAGCTAAGGTTATTAGAGATTGTGTTGATAATCTCAAAGACTCTACCGACGAGCTCCATAGAGCTATCGCCGGATTCAAAAGCTTGAGTCGTGCTAAAGATGCCAACTTTCAGGTTATTTAAACTTCTACTTAAGCCCAAAAATTTTAAATTTATATGTTTTCGTAAATTTTATTCAAATTCATATTATAAACACTATTCTACCTCCGATAAACTTAAAATCAACTAAAAAAATATAAAAGACATGATCTTCGTACACTTCTGGTTTGACCATAAGTTTTAAATACGAAAGTCAACCTAGAGACCTAAAAAAATATATATTTAATCAAATAATTTTAACATTTTTTATAAATGAAATTATGTTGACATTATAGTGGTCCAAGGTCCAATGAGTAATTATCAATTTTAATTAAACCCTTTAAATAAGCTAAGGTAGATTGGGTTAAATTTAATATTTGTATTCATATTGTGAACACAGTTACATAGTATCAAAACATGGGTGAGTGCTGCAGAGACCGATGCTATGACTTGCAGCGATGGGTTGAGCGGCGGCGCTGGTCGGAAGGTGAGGTCGAAGGTGAAGCAAGAGGTGAGGAAGAGTGTTGCGGTTGTGGTGAGGCATATCAGTAACGCCTTGTCCCTCCTCACCACCTTCAAATACAAATCCTGAGCTCAGCTCAGCTTTTCAAACAACATAAACATCCAAACAACTGAGCTGCATCAACTTGTTTGCAGCTTCAATCTTGTTTTAATGTCATTTCAAAGTATAAAATATGTTTCTTTCGTG

mRNA sequence

TGATATGGAATTTCAAATCCTAAAATCGTTACTAACCCTTTTTTTTTTCGTTTCAATTACGTTCACCACCTCCGTCGTGGCTTCTTCTTCTTCCATTATCCGTCCAGTGAAACCATACATCAAAAAGGCTTGCAAAACGACGCCGTATCCACGCCTTTGCGAGATCTCCCTCACTTTACACGCTTCACAAACCAAAAAAACACAACAAGAGTTCTGTCGTGCGGCCATGGCTTCTTCTCTCAAAACTACACGAGATGCCACCTCCATAATCTCCAAGTTATCTCGCCAAAAGATGTCTACCTATGAAGCTAAGGTTATTAGAGATTGTGTTGATAATCTCAAAGACTCTACCGACGAGCTCCATAGAGCTATCGCCGGATTCAAAAGCTTGAGTCGTGCTAAAGATGCCAACTTTCAGTTACATAGTATCAAAACATGGGTGAGTGCTGCAGAGACCGATGCTATGACTTGCAGCGATGGGTTGAGCGGCGGCGCTGGTCGGAAGGTGAGGTCGAAGGTGAAGCAAGAGGTGAGGAAGAGTGTTGCGGTTGTGGTGAGGCATATCAGTAACGCCTTGTCCCTCCTCACCACCTTCAAATACAAATCCTGAGCTCAGCTCAGCTTTTCAAACAACATAAACATCCAAACAACTGAGCTGCATCAACTTGTTTGCAGCTTCAATCTTGTTTTAATGTCATTTCAAAGTATAAAATATGTTTCTTTCGTG

Coding sequence (CDS)

ATGGAATTTCAAATCCTAAAATCGTTACTAACCCTTTTTTTTTTCGTTTCAATTACGTTCACCACCTCCGTCGTGGCTTCTTCTTCTTCCATTATCCGTCCAGTGAAACCATACATCAAAAAGGCTTGCAAAACGACGCCGTATCCACGCCTTTGCGAGATCTCCCTCACTTTACACGCTTCACAAACCAAAAAAACACAACAAGAGTTCTGTCGTGCGGCCATGGCTTCTTCTCTCAAAACTACACGAGATGCCACCTCCATAATCTCCAAGTTATCTCGCCAAAAGATGTCTACCTATGAAGCTAAGGTTATTAGAGATTGTGTTGATAATCTCAAAGACTCTACCGACGAGCTCCATAGAGCTATCGCCGGATTCAAAAGCTTGAGTCGTGCTAAAGATGCCAACTTTCAGTTACATAGTATCAAAACATGGGTGAGTGCTGCAGAGACCGATGCTATGACTTGCAGCGATGGGTTGAGCGGCGGCGCTGGTCGGAAGGTGAGGTCGAAGGTGAAGCAAGAGGTGAGGAAGAGTGTTGCGGTTGTGGTGAGGCATATCAGTAACGCCTTGTCCCTCCTCACCACCTTCAAATACAAATCCTGA

Protein sequence

MEFQILKSLLTLFFFVSITFTTSVVASSSSIIRPVKPYIKKACKTTPYPRLCEISLTLHASQTKKTQQEFCRAAMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLKDSTDELHRAIAGFKSLSRAKDANFQLHSIKTWVSAAETDAMTCSDGLSGGAGRKVRSKVKQEVRKSVAVVVRHISNALSLLTTFKYKS
Homology
[Close]
BLAST of CmaCh04G003310 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 5.2e-22
Identity = 65/187 (34.76%), Postives = 105/187 (56.15%), Query Frame = 1 

Query: 17  SITFTTSVVASSSSIIRPVKPYIKKACKTTPYPRLCEISLTLHASQTKKTQQEFCRAAMA 76
           ++ F+  +V  +++   P   +IK +C  T YP +CE SL+ +A   +   QE    A+ 
Sbjct: 7   TLVFSALLVILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQ 66

Query: 77  SSLKTTRDATSIISKLSRQK-MSTYEAKVIRDCVDNLKDSTDELHRAIAGFKSLSRAK-- 136
            SL  T+ A + + +L++ K +   +   I DC++ ++DS D + R+    K+LS AK  
Sbjct: 67  VSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGN 126

Query: 137 DANFQLHSIKTWVSAAETDAMTCSDGLSGGAGRKVRSKVKQEVRKSVAVVVRHISNALSL 196
           D  F++ +++TWVSAA TD  TC DG    AG+ +  K+K+ VR  V  V R  SNAL+L
Sbjct: 127 DFTFRMSNVETWVSAALTDETTCMDGF---AGKGMDGKIKESVRAQVVAVARVTSNALAL 186

Query: 197 LTTFKYK 201
           +  F  K
Sbjct: 187 VNNFAAK 190

[Close]
BLAST of CmaCh04G003310 vs. TrEMBL
Match: A0A0A0L671_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176250 PE=4 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 6.1e-62
Identity = 133/203 (65.52%), Postives = 161/203 (79.31%), Query Frame = 1 

Query: 1   MEFQILKSLLTL---FFFVSITFTTSVVASSSSIIRPVKPYIKKACKTTPYPRLCEISLT 60
           ME QILKS LTL   FF +  TFT S V+SSSS +RPV+P+I+KACK TPYPRLCE +L+
Sbjct: 1   MESQILKSSLTLLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALS 60

Query: 61  LHASQTKKTQQEFCRAAMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLKDSTD 120
           L+ASQTK+ QQE CRAAM SSLK  ++ATSIISKLSR+KMS YEA+VI DC+DNLKDS D
Sbjct: 61  LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSVD 120

Query: 121 ELHRAIAGFKSLSRAKDANFQLHSIKTWVSAAETDAMTCSDGLSGGAGRKVRSKVKQEVR 180
           EL RA    KSLSR+KD +FQL+SIKTW+SAA+TD +TC+DGLSGG+G KV   +K++V+
Sbjct: 121 ELRRASTAIKSLSRSKDVDFQLNSIKTWMSAAQTDVITCTDGLSGGSGWKVSKMLKKDVK 180

Query: 181 KSVAVVVRHISNALSLLTTFKYK 201
                VVR ISNAL L+  F YK
Sbjct: 181 NCSINVVRQISNALFLINNFNYK 203

[Close]
BLAST of CmaCh04G003310 vs. TrEMBL
Match: F6HAT8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0022g00940 PE=4 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 3.5e-25
Identity = 79/194 (40.72%), Postives = 120/194 (61.86%), Query Frame = 1 

Query: 3   FQILKSLL-TLFFFVSITFTTSVVASSSSIIRPVKPYIKKACKTTPYPRLCEISLTLHAS 62
           F +L SL  TL  F SIT T+S   S+ S+    K +IK +C +T YP +C  SL+ +AS
Sbjct: 12  FHVLSSLFFTLLLFNSITLTSSANTSTISLTIS-KQFIKTSCNSTTYPGVCYKSLSSYAS 71

Query: 63  QTKKTQQEFCRAAMASSLKTTRDATSIISKL-SRQKMSTYEAKVIRDCVDNLKDSTDELH 122
           + + +  +    A+  SLK  R+A+S I+ L  R+ M+  E  +++DCV+N  D  DEL 
Sbjct: 72  KVQTSPLKLANVALTVSLKAARNASSTITLLLERKGMTRMETSIVKDCVENFGDCIDELQ 131

Query: 123 RAIAGFKSLSRAKDANFQLHSIKTWVSAAETDAMTCSDGLSGGAGRKVRSKVKQEVRKSV 182
           +++  FKSL   K+  FQ+ +IKTWVSAA TD  TCSDG     G+KV S ++Q+++  +
Sbjct: 132 QSLQEFKSLGGGKNVAFQMANIKTWVSAALTDEYTCSDGFE---GQKVSSSLQQQIKNYI 191

Query: 183 AVVVRHISNALSLL 195
           + V +  SNAL+L+
Sbjct: 192 SNVAKITSNALALI 201

[Close]
BLAST of CmaCh04G003310 vs. TrEMBL
Match: A5BT23_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_005236 PE=4 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 3.5e-25
Identity = 79/194 (40.72%), Postives = 120/194 (61.86%), Query Frame = 1 

Query: 3   FQILKSLL-TLFFFVSITFTTSVVASSSSIIRPVKPYIKKACKTTPYPRLCEISLTLHAS 62
           F +L SL  TL  F SIT T+S   S+ S+    K +IK +C +T YP +C  SL+ +AS
Sbjct: 12  FHVLSSLFFTLLLFNSITLTSSANTSTISLTIS-KQFIKTSCNSTTYPGVCYKSLSSYAS 71

Query: 63  QTKKTQQEFCRAAMASSLKTTRDATSIISKL-SRQKMSTYEAKVIRDCVDNLKDSTDELH 122
           + + +  +    A+  SLK  R+A+S I+ L  R+ M+  E  +++DCV+N  D  DEL 
Sbjct: 72  KVQTSPLKLANVALTVSLKAARNASSTITLLLERKGMTRMETSIVKDCVENFGDCIDELQ 131

Query: 123 RAIAGFKSLSRAKDANFQLHSIKTWVSAAETDAMTCSDGLSGGAGRKVRSKVKQEVRKSV 182
           +++  FKSL   K+  FQ+ +IKTWVSAA TD  TCSDG     G+KV S ++Q+++  +
Sbjct: 132 QSLQEFKSLGGGKNVAFQMANIKTWVSAALTDEYTCSDGFE---GQKVSSSLQQQIKNYI 191

Query: 183 AVVVRHISNALSLL 195
           + V +  SNAL+L+
Sbjct: 192 SNVAKITSNALALI 201

[Close]
BLAST of CmaCh04G003310 vs. TrEMBL
Match: B9R9Q8_RICCO (21 kDa protein, putative OS=Ricinus communis GN=RCOM_1500020 PE=4 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 6.0e-25
Identity = 71/192 (36.98%), Postives = 114/192 (59.38%), Query Frame = 1 

Query: 9   LLTLFFFVSITFTTSVVASSSSIIRPVKPYIKKACKTTPYPRLCEISLTLHASQTKKTQQ 68
           L+T   F+S T  +  + ++ +     K Y+K AC +T YP++C  +L+ + S  +    
Sbjct: 18  LITFLVFISNTQNSLAITTTKTSKTTYKNYLKTACNSTTYPKICYNTLSPYTSTIQTNDL 77

Query: 69  EFCRAAMASSLKTTRDATSIISKLSRQK-MSTYEAKVIRDCVDNLKDSTDELHRAIAGFK 128
           + C AA+  +LK   + ++++  LS+QK +S  E  VI+DC   ++DS DEL +++   K
Sbjct: 78  KLCNAALTITLKAASNTSAMVKSLSKQKGLSKGEVAVIKDCQYEIEDSVDELKQSLKALK 137

Query: 129 SLSRAKDANFQLHSIKTWVSAAETDAMTCSDGLSGGAGRKVRSKVKQEVRKSVAVVVRHI 188
           +L  + D  FQ+ +IKTW+SAA TD  TC+DG     G KV SKVK +++KS+  V R  
Sbjct: 138 NLKGSADMEFQIDNIKTWISAAITDENTCTDGFE---GMKVSSKVKSKIKKSIVNVNRLT 197

Query: 189 SNALSLLTTFKY 200
           SNAL+L+    Y
Sbjct: 198 SNALALINKLSY 206

[Close]
BLAST of CmaCh04G003310 vs. TrEMBL
Match: M5X0K1_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011478mg PE=4 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 3.0e-24
Identity = 75/192 (39.06%), Postives = 112/192 (58.33%), Query Frame = 1 

Query: 10  LTLFFFVSITFTTSVVASSSSIIRPVKPYIKKACKTTPYPRLCEISLTLHASQTKKTQQE 69
           + L  F+    TT   +SSS   +  K Y+K AC TT YP +C  SL+ +AS+ K   Q+
Sbjct: 23  ILLLIFMPNMHTTLATSSSS---QTYKTYVKTACNTTTYPLICYKSLSSYASKVKSDPQK 82

Query: 70  FCRAAMASSLKTTRDATSIISKLSRQK-MSTYEAKVIRDCVDNLKDSTDELHRAIAGFKS 129
            C  A++ +LK  ++A+S++SKL +   ++  E  V++DC++N+KDS DEL ++++   +
Sbjct: 83  LCTYALSVTLKAAKNASSVVSKLYKNTGLTPSEKGVVKDCIENIKDSIDELKQSVSSMSN 142

Query: 130 LS-RAKDANFQLHSIKTWVSAAETDAMTCSDGLSGGAGRKVRSKVKQEVRKSVAVVVRHI 189
           L     D   QL  IKTWVSA  TD  TC+DG     G KV + VK  +R S+    R  
Sbjct: 143 LGVSGSDVQLQLDDIKTWVSAVITDDATCTDGFD---GVKVSAAVKTAIRNSIVNAARLA 202

Query: 190 SNALSLLTTFKY 200
           SNALSL+ +  Y
Sbjct: 203 SNALSLIDSLIY 208

[Close]
BLAST of CmaCh04G003310 vs. TAIR10
Match: AT5G62350.1 (AT5G62350.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 102.4 bits (254), Expect = 3.2e-22
Identity = 66/201 (32.84%), Postives = 109/201 (54.23%), Query Frame = 1 

Query: 1   MEFQILKSLLTLFFFVSITFTTSVVASSSSIIRPVKPYIKKACKTTPYPRLCEISLTLHA 60
           M  Q L  LL++ + +S+  T +  AS +   +    +I+ +CKTT YP LC  SL+++A
Sbjct: 1   MAKQYLFVLLSISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYA 60

Query: 61  SQTKKTQQEFCRAAMASSLKTTRDATSIISKLSRQK-MSTYEAKVIRDCVDNLKDSTDEL 120
           +  + + +     A+A +L   +     +S+L+R K +   E + I+DCV+ + D+ D L
Sbjct: 61  NDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRL 120

Query: 121 HRAIAGFKSLSRAKDAN---FQLHSIKTWVSAAETDAMTCSDGLSGGAGRKVRSKVKQEV 180
            +++   K    AKD +   + + + +TW SAA TD  TCSDG S   GR +  ++K  V
Sbjct: 121 TKSVQELKLCGSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFS---GRVMDGRIKNSV 180

Query: 181 RKSVAVVVRHISNALSLLTTF 198
           R  +  V    SNALSL+  F
Sbjct: 181 RARIMNVGHETSNALSLINAF 198

[Close]
BLAST of CmaCh04G003310 vs. TAIR10
Match: AT5G51520.1 (AT5G51520.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 100.1 bits (248), Expect = 1.6e-21
Identity = 66/205 (32.20%), Postives = 110/205 (53.66%), Query Frame = 1 

Query: 4   QILKSLLTLFFFVSITFTTSVVASSSSIIRPVKPYIKKACKTTPYPRLCEISLTLHASQT 63
           Q+L SL  +F F + T       S+SS  +  K ++K AC +T YP  C  SL+ ++S  
Sbjct: 3   QVLYSLTIVFVFFASTNIQKTSGSASSYSQNHKTFVKTACNSTTYPDKCYKSLSSYSSNI 62

Query: 64  KKTQQEFCRAAMASSLKTTRDATSIISKLSRQKMSTY---------EAKVIRDCVDNLKD 123
           K    + C  A+  ++K+ ++ATS++SKL +    +          EA +++DC++ +KD
Sbjct: 63  KSDPIKLCTTALNLNVKSAKEATSVVSKLLKMSQKSTAGRKGKMLPEALILKDCLEEMKD 122

Query: 124 STDELHRAIAGFKSLSRAKDANFQLHSIKTWVSAAETDAMTCSDGLSGGAGRKVRSKVKQ 183
           +  EL +AI   K+L         + +++TWVS+A TD  TC+DG       KV  + K+
Sbjct: 123 TIIELKQAITEMKNLQDGGSMAEHITNVRTWVSSALTDEGTCTDGFE---EVKVNKETKK 182

Query: 184 EVRKSVAVVVRHISNALSLLTTFKY 200
           +V K V  +    SN L+L+T  +Y
Sbjct: 183 KVNKVVEELATTTSNTLALITNLRY 204

[Close]
BLAST of CmaCh04G003310 vs. TAIR10
Match: AT4G25250.1 (AT4G25250.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 98.2 bits (243), Expect = 6.1e-21
Identity = 60/191 (31.41%), Postives = 110/191 (57.59%), Query Frame = 1 

Query: 10  LTLFFFVSITFTTSVVASSSSIIRPVKPYIKKACKTTPYPRLCEISLTLHASQTKKTQQE 69
           LTL  F++ +      A+     +    Y+K AC +T YP +C   L+ ++S  K    +
Sbjct: 9   LTLMVFINSSNFPKTAATPPGTYQNHTTYVKTACNSTTYPTMCYNCLSSYSSTIKSDPIK 68

Query: 70  FCRAAMASSLKTTRDATSIISKLSRQKMS--TYEAKVIRDCVDNLKDSTDELHRAIAGFK 129
            C  ++  ++K+ ++AT ++S L ++  +  ++E  +++DCVD +KD+ DEL +A+A  K
Sbjct: 69  LCTTSLNLNVKSAKNATLVVSNLLQKAKAAKSHEVSILKDCVDEMKDTIDELKQAVAEMK 128

Query: 130 SL-SRAKDANFQLHSIKTWVSAAETDAMTCSDGLSGGAGRKVRSKVKQEVRKSVAVVVRH 189
            +    K     L ++KTWVS+A TD  TC+DG   G   +V  + K++V+K+++ + + 
Sbjct: 129 YVRGGGKTTEEHLKNVKTWVSSALTDEGTCTDGFEEG---RVNVETKKKVKKAISELSKT 188

Query: 190 ISNALSLLTTF 198
            SN L+LLT +
Sbjct: 189 TSNTLALLTHY 196

[Close]
BLAST of CmaCh04G003310 vs. TAIR10
Match: AT5G62360.1 (AT5G62360.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 89.0 bits (219), Expect = 3.7e-18
Identity = 64/197 (32.49%), Postives = 106/197 (53.81%), Query Frame = 1 

Query: 3   FQILKSLLTLFFFVSITFTTSVVASSSSIIRPVKPYIKKACKTTPYPRLCEISLTLHASQ 62
           F I+ ++L L    + T TT+   +          ++K +C  T YPRLC  SL+ HAS 
Sbjct: 17  FLIIIAMLKLVHTTTTTTTTTTTNTE---------FVKSSCTFTTYPRLCFSSLSTHASL 76

Query: 63  TKKTQQEFCRAAMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLKDSTDELHRA 122
            + + +    AA+  +L + +  ++++ +LS  ++   E   +RDCV+ L D+ +EL ++
Sbjct: 77  IQTSPKLMAHAALNITLASAKVTSAMMVRLSNSRLKPKEVSAMRDCVEELGDTLEELRKS 136

Query: 123 IAGFKSLSRAKDANFQLH--SIKTWVSAAETDAMTCSDGLSGGAGRKVRSKVKQEVRKSV 182
           I     LS    +N++++   I+TWVSAA TD  TC+DG     G  +  KVK  VR  +
Sbjct: 137 IGEMCQLS---GSNYEVYISDIQTWVSAALTDVNTCTDGFE---GEDMDGKVKVLVRGRI 196

Query: 183 AVVVRHISNALSLLTTF 198
            V+    SNAL+L+  F
Sbjct: 197 LVIAHLTSNALALINHF 198

[Close]
BLAST of CmaCh04G003310 vs. TAIR10
Match: AT1G62770.1 (AT1G62770.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 80.9 bits (198), Expect = 1.0e-15
Identity = 66/204 (32.35%), Postives = 110/204 (53.92%), Query Frame = 1 

Query: 6   LKSLLTLFFFVSITFTTSVVASSSSIIRPVKPYIKKACKTTPYPRLCEISLTLHASQTK- 65
           LK+ + L   +SIT   S  A+ +        +I  +C+TT YP LC  +L+ +A++ + 
Sbjct: 3   LKNTIFLVILLSITILQSSSATPNR--SESDQFIVSSCQTTQYPSLCVHTLSAYATKIRH 62

Query: 66  KTQQEFCRAAMASSLKTTRDATSIISKLSRQ--KMSTYEAKVIRDCVDNLKDSTDELHRA 125
              Q+  + A+  SL   +  T  ++KL+++  K    E   I+DC++ L +S D L ++
Sbjct: 63  NNDQDLAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQS 122

Query: 126 IAGFKSLSRA------KDANFQLHSIKTWVSAAETDAMTCSDGLSGGAGRKVRSKVKQEV 185
           +   K L+RA      +D  +++ +++TWVSAA TD  TC DG S    R +  KVK+ +
Sbjct: 123 V---KELARAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFS---ERAMGGKVKRLI 182

Query: 186 RKSVAVVVRHISNALSLLTTFKYK 201
           R  V  V +  SNAL+L+  F  K
Sbjct: 183 RYKVVHVAQVTSNALALVNQFAEK 198

[Close]
BLAST of CmaCh04G003310 vs. NCBI nr
Match: gi|659077238|ref|XP_008439099.1| (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 252.3 bits (643), Expect = 7.1e-64
Identity = 139/202 (68.81%), Postives = 161/202 (79.70%), Query Frame = 1 

Query: 1   MEFQILKSLLTLFFFVSI--TFTTSVVASSSSIIRPVKPYIKKACKTTPYPRLCEISLTL 60
           ME QILKS LTLFFF  I  TFT S VASSSS +RPVKP+I+KACK TPYPRLCE +L+L
Sbjct: 1   MESQILKSSLTLFFFFIILTTFTPSAVASSSSTVRPVKPHIRKACKPTPYPRLCETALSL 60

Query: 61  HASQTKKTQQEFCRAAMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLKDSTDE 120
           +ASQTK+ QQE CRAAM SSLK  ++ATSIISKLSR+KMS YE +VI DC+D+LKDS DE
Sbjct: 61  YASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYETEVIGDCIDDLKDSVDE 120

Query: 121 LHRAIAGFKSLSRAKDANFQLHSIKTWVSAAETDAMTCSDGLSGGAGRKVRSKVKQEVRK 180
           L RA    KSLSR+KD +FQL+SIKTW SAA TD +TC+DGLSGG+G KV  KVK+EV+ 
Sbjct: 121 LRRASTAIKSLSRSKDVDFQLNSIKTWTSAALTDIITCTDGLSGGSGWKVNKKVKKEVKN 180

Query: 181 SVAVVVRHISNALSLLTTFKYK 201
               VVR ISNALSL+  F YK
Sbjct: 181 CSINVVRQISNALSLINNFNYK 202

[Close]
BLAST of CmaCh04G003310 vs. NCBI nr
Match: gi|449460810|ref|XP_004148137.1| (PREDICTED: 21 kDa protein-like [Cucumis sativus])

HSP 1 Score: 245.4 bits (625), Expect = 8.7e-62
Identity = 133/203 (65.52%), Postives = 161/203 (79.31%), Query Frame = 1 

Query: 1   MEFQILKSLLTL---FFFVSITFTTSVVASSSSIIRPVKPYIKKACKTTPYPRLCEISLT 60
           ME QILKS LTL   FF +  TFT S V+SSSS +RPV+P+I+KACK TPYPRLCE +L+
Sbjct: 1   MESQILKSSLTLLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALS 60

Query: 61  LHASQTKKTQQEFCRAAMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLKDSTD 120
           L+ASQTK+ QQE CRAAM SSLK  ++ATSIISKLSR+KMS YEA+VI DC+DNLKDS D
Sbjct: 61  LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSVD 120

Query: 121 ELHRAIAGFKSLSRAKDANFQLHSIKTWVSAAETDAMTCSDGLSGGAGRKVRSKVKQEVR 180
           EL RA    KSLSR+KD +FQL+SIKTW+SAA+TD +TC+DGLSGG+G KV   +K++V+
Sbjct: 121 ELRRASTAIKSLSRSKDVDFQLNSIKTWMSAAQTDVITCTDGLSGGSGWKVSKMLKKDVK 180

Query: 181 KSVAVVVRHISNALSLLTTFKYK 201
                VVR ISNAL L+  F YK
Sbjct: 181 NCSINVVRQISNALFLINNFNYK 203

[Close]
BLAST of CmaCh04G003310 vs. NCBI nr
Match: gi|470146900|ref|XP_004309057.1| (PREDICTED: 21 kDa protein-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 125.2 bits (313), Expect = 1.3e-25
Identity = 77/192 (40.10%), Postives = 116/192 (60.42%), Query Frame = 1 

Query: 9   LLTLFFFVSITFTTSVVASSSSIIRPVKPYIKKACKTTPYPRLCEISLTLHASQTKKTQQ 68
           L  L  F+    TT+  +SSS   R  K Y+K AC +T YP++C  SL+ +A+  K   Q
Sbjct: 7   LFALLLFILFINTTTATSSSS---RTYKTYVKTACNSTTYPQICYKSLSSYATSIKSNPQ 66

Query: 69  EFCRAAMASSLKTTRDATSIISKLSRQK-MSTYEAKVIRDCVDNLKDSTDELHRAIAGFK 128
           + C  A++ +LK  + + S +SKL+++K ++  EA V+  C++ +KDS DEL +++   K
Sbjct: 67  KLCSYALSVTLKAAKTSASTVSKLAKKKGITKAEAGVVGACIETIKDSVDELKQSVTAMK 126

Query: 129 SLSRAKDANFQLHSIKTWVSAAETDAMTCSDGLSGGAGRKVRSKVKQEVRKSVAVVVRHI 188
           +L  A D  +QL +IKTWVSA+ TD  TC+DG      +KV + VK  +RKS+  V R  
Sbjct: 127 NLGGA-DMQYQLDNIKTWVSASLTDEDTCTDGFD---EQKVSTAVKNTIRKSILTVARLA 186

Query: 189 SNALSLLTTFKY 200
           SNALSL+    Y
Sbjct: 187 SNALSLIDNLNY 191

[Close]
BLAST of CmaCh04G003310 vs. NCBI nr
Match: gi|225466055|ref|XP_002263511.1| (PREDICTED: 21 kDa protein [Vitis vinifera])

HSP 1 Score: 123.2 bits (308), Expect = 5.0e-25
Identity = 79/194 (40.72%), Postives = 120/194 (61.86%), Query Frame = 1 

Query: 3   FQILKSLL-TLFFFVSITFTTSVVASSSSIIRPVKPYIKKACKTTPYPRLCEISLTLHAS 62
           F +L SL  TL  F SIT T+S   S+ S+    K +IK +C +T YP +C  SL+ +AS
Sbjct: 12  FHVLSSLFFTLLLFNSITLTSSANTSTISLTIS-KQFIKTSCNSTTYPGVCYKSLSSYAS 71

Query: 63  QTKKTQQEFCRAAMASSLKTTRDATSIISKL-SRQKMSTYEAKVIRDCVDNLKDSTDELH 122
           + + +  +    A+  SLK  R+A+S I+ L  R+ M+  E  +++DCV+N  D  DEL 
Sbjct: 72  KVQTSPLKLANVALTVSLKAARNASSTITLLLERKGMTRMETSIVKDCVENFGDCIDELQ 131

Query: 123 RAIAGFKSLSRAKDANFQLHSIKTWVSAAETDAMTCSDGLSGGAGRKVRSKVKQEVRKSV 182
           +++  FKSL   K+  FQ+ +IKTWVSAA TD  TCSDG     G+KV S ++Q+++  +
Sbjct: 132 QSLQEFKSLGGGKNVAFQMANIKTWVSAALTDEYTCSDGFE---GQKVSSSLQQQIKNYI 191

Query: 183 AVVVRHISNALSLL 195
           + V +  SNAL+L+
Sbjct: 192 SNVAKITSNALALI 201

[Close]
BLAST of CmaCh04G003310 vs. NCBI nr
Match: gi|147860755|emb|CAN79278.1| (hypothetical protein VITISV_005236 [Vitis vinifera])

HSP 1 Score: 123.2 bits (308), Expect = 5.0e-25
Identity = 79/194 (40.72%), Postives = 120/194 (61.86%), Query Frame = 1 

Query: 3   FQILKSLL-TLFFFVSITFTTSVVASSSSIIRPVKPYIKKACKTTPYPRLCEISLTLHAS 62
           F +L SL  TL  F SIT T+S   S+ S+    K +IK +C +T YP +C  SL+ +AS
Sbjct: 12  FHVLSSLFFTLLLFNSITLTSSANTSTISLTIS-KQFIKTSCNSTTYPGVCYKSLSSYAS 71

Query: 63  QTKKTQQEFCRAAMASSLKTTRDATSIISKL-SRQKMSTYEAKVIRDCVDNLKDSTDELH 122
           + + +  +    A+  SLK  R+A+S I+ L  R+ M+  E  +++DCV+N  D  DEL 
Sbjct: 72  KVQTSPLKLANVALTVSLKAARNASSTITLLLERKGMTRMETSIVKDCVENFGDCIDELQ 131

Query: 123 RAIAGFKSLSRAKDANFQLHSIKTWVSAAETDAMTCSDGLSGGAGRKVRSKVKQEVRKSV 182
           +++  FKSL   K+  FQ+ +IKTWVSAA TD  TCSDG     G+KV S ++Q+++  +
Sbjct: 132 QSLQEFKSLGGGKNVAFQMANIKTWVSAALTDEYTCSDGFE---GQKVSSSLQQQIKNYI 191

Query: 183 AVVVRHISNALSLL 195
           + V +  SNAL+L+
Sbjct: 192 SNVAKITSNALALI 201

The following BLAST results are available for this feature:
BLAST of CmaCh04G003310 vs. Swiss-Prot
Match NameE-valueIdentityDescription
21KD_DAUCA5.2e-2234.7621 kDa protein OS=Daucus carota PE=2 SV=1[more]
BLAST of CmaCh04G003310 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L671_CUCSA6.1e-6265.52Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176250 PE=4 SV=1[more]
F6HAT8_VITVI3.5e-2540.72Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0022g00940 PE=4 SV=... [more]
A5BT23_VITVI3.5e-2540.72Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_005236 PE=4 SV=1[more]
B9R9Q8_RICCO6.0e-2536.9821 kDa protein, putative OS=Ricinus communis GN=RCOM_1500020 PE=4 SV=1[more]
M5X0K1_PRUPE3.0e-2439.06Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011478mg PE=4 SV=1[more]
BLAST of CmaCh04G003310 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G62350.13.2e-2232.84 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G51520.11.6e-2132.20 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT4G25250.16.1e-2131.41 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G62360.13.7e-1832.49 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G62770.11.0e-1532.35 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
BLAST of CmaCh04G003310 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659077238|ref|XP_008439099.1|7.1e-6468.81PREDICTED: 21 kDa protein-like [Cucumis melo][more]
gi|449460810|ref|XP_004148137.1|8.7e-6265.52PREDICTED: 21 kDa protein-like [Cucumis sativus][more]
gi|470146900|ref|XP_004309057.1|1.3e-2540.10PREDICTED: 21 kDa protein-like [Fragaria vesca subsp. vesca][more]
gi|225466055|ref|XP_002263511.1|5.0e-2540.72PREDICTED: 21 kDa protein [Vitis vinifera][more]
gi|147860755|emb|CAN79278.1|5.0e-2540.72hypothetical protein VITISV_005236 [Vitis vinifera][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR006501Pectinesterase_inhib_dom
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G003310.1CmaCh04G003310.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 37..197
score: 1.1
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 38..193
score: 1.7
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 34..193
score: 1.2
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 12..195
score: 7.2
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 37..197
score: 7.06
NoneNo IPR availablePANTHERPTHR31080FAMILY NOT NAMEDcoord: 1..194
score: 3.6
NoneNo IPR availablePANTHERPTHR31080:SF15SUBFAMILY NOT NAMEDcoord: 1..194
score: 3.6

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh04G003310Cucsa.251560Cucumber (Gy14) v1cgycmaB0722
CmaCh04G003310Cla006265Watermelon (97103) v1cmawmB707
CmaCh04G003310Csa3G176250Cucumber (Chinese Long) v2cmacuB703
CmaCh04G003310MELO3C006820Melon (DHL92) v3.5.1cmameB692
CmaCh04G003310CSPI03G16020Wild cucumber (PI 183967)cmacpiB712
CmaCh04G003310Cp4.1LG14g05620Cucurbita pepo (Zucchini)cmacpeB715
CmaCh04G003310MELO3C006820.2Melon (DHL92) v3.6.1cmamedB779
CmaCh04G003310CsaV3_3G016220Cucumber (Chinese Long) v3cmacucB0838
CmaCh04G003310CsGy3G016100Cucumber (Gy14) v2cgybcmaB417
CmaCh04G003310Carg08008Silver-seed gourdcarcmaB0529
CmaCh04G003310Carg16733Silver-seed gourdcarcmaB1209
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh04G003310CmaCh16G001850Cucurbita maxima (Rimu)cmacmaB351
The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh04G003310Watermelon (97103) v2cmawmbB767
CmaCh04G003310Wax gourdcmawgoB0856
CmaCh04G003310Cucurbita moschata (Rifu)cmacmoB701
CmaCh04G003310Cucurbita moschata (Rifu)cmacmoB728
CmaCh04G003310Watermelon (Charleston Gray)cmawcgB663
CmaCh04G003310Cucurbita pepo (Zucchini)cmacpeB720
CmaCh04G003310Bottle gourd (USVL1VR-Ls)cmalsiB688