Bhi01G000901 (gene) Wax gourd

Overview
NameBhi01G000901
Typegene
OrganismBenincasa hispida (Wax gourd)
Description21 kDa protein-like
Locationchr1 : 25015837 .. 25017014 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GACGTGTCTTCCACAACTATAAATATAGTAAAAATCTCCCGGCAACCCTAATAGATTCACTAGAGAAAGAGATGGAATCTCAAATCCTAAAATCCTCACTAACCCTTTTCTTCTTCATTGTTTTAACAACATTCACTACCTCCGCCGCGGCCTCTTCTTCCTCCACCGTCCGGCCAGTACAACCACACATCAGAAAGGCATGCAAGCCGACGCCATATCCGCGCCTTTGCGAGACTGCACTCTCTTTATACGCTTCACAGACCAAAAGAAACCAGCAAGAACTATGCAGGGCGGCCATGGTTTCTTCTCTCAAAGCTGCACAAAACGCCACCTCCATCATCTCCAAGATCTCCCGTCGGAAGTTGTCACCCTATGAAGCCGAAGTCATCGGAGATTGCATCGATAATCTCAATGACTCCGTCGACGAGATCCGTCGAGCTACCACTGCCATTAAAAAATTGAGTCGTTCTAAGGATGTGGATTTTCAGGTTGTTAAAACTACTACTTAATCCTATTAACGGTTTAATTTCATATTCTTTTTAGGTTAAAAAACATTTTTGTCTCTGAATTTTCGGGAAAATAATTAACAATTTTAGTTGATAATGATGTGACCCCTATATTTTTAAATTTGTAATTCTTGATTTTTAGTCCTTATGATCTATAATTTGTAATGACTTAGTCCTATCGTGAAAAAAACATTCATAAGGACTAAAACATTACAGATGTTTTTAGAGACTAAACCGTTTCTTTCATAAAAGTAAATGTGTTTAACCAAATTTATTCATATTCTTAATGCAGTTGAGTAGTATAAAGACGTGGATGAGCGCAGCAGAGACCGATGTTATCACGTGCACCGACGGGTTGAGCGGCGCGGCGGGGCGGAAGGTGAGGACGAAGGTGAAGAAAGAGGTGAAGAATTGTAGTGTGATTGTGGTAAGGCAGATTAGTAATGCCTTGTCTCTCATCAACAACTTCAACTACAAACACTGAGCTTCTGAGTTTCTGAATTTACTACTTCAATGGTCACCTATCAAATACAAAATTTGTACTTCCAACTTTATCCATTTCAAACTATAAAATATGTTTCCTTTTCTGTGTTCTTTCATCATCTCAATTCTTTCTATAATTTTTTATGTCTATTTTGGTTATACATTATTACAATTTTACTTTAATTTGAT

mRNA sequence

GACGTGTCTTCCACAACTATAAATATAGTAAAAATCTCCCGGCAACCCTAATAGATTCACTAGAGAAAGAGATGGAATCTCAAATCCTAAAATCCTCACTAACCCTTTTCTTCTTCATTGTTTTAACAACATTCACTACCTCCGCCGCGGCCTCTTCTTCCTCCACCGTCCGGCCAGTACAACCACACATCAGAAAGGCATGCAAGCCGACGCCATATCCGCGCCTTTGCGAGACTGCACTCTCTTTATACGCTTCACAGACCAAAAGAAACCAGCAAGAACTATGCAGGGCGGCCATGGTTTCTTCTCTCAAAGCTGCACAAAACGCCACCTCCATCATCTCCAAGATCTCCCGTCGGAAGTTGTCACCCTATGAAGCCGAAGTCATCGGAGATTGCATCGATAATCTCAATGACTCCGTCGACGAGATCCGTCGAGCTACCACTGCCATTAAAAAATTGAGTCGTTCTAAGGATGTGGATTTTCAGTTGAGTAGTATAAAGACGTGGATGAGCGCAGCAGAGACCGATGTTATCACGTGCACCGACGGGTTGAGCGGCGCGGCGGGGCGGAAGGTGAGGACGAAGGTGAAGAAAGAGGTGAAGAATTGTAGTGTGATTGTGGTAAGGCAGATTAGTAATGCCTTGTCTCTCATCAACAACTTCAACTACAAACACTGAGCTTCTGAGTTTCTGAATTTACTACTTCAATGGTCACCTATCAAATACAAAATTTGTACTTCCAACTTTATCCATTTCAAACTATAAAATATGTTTCCTTTTCTGTGTTCTTTCATCATCTCAATTCTTTCTATAATTTTTTATGTCTATTTTGGTTATACATTATTACAATTTTACTTTAATTTGAT

Coding sequence (CDS)

ATGGAATCTCAAATCCTAAAATCCTCACTAACCCTTTTCTTCTTCATTGTTTTAACAACATTCACTACCTCCGCCGCGGCCTCTTCTTCCTCCACCGTCCGGCCAGTACAACCACACATCAGAAAGGCATGCAAGCCGACGCCATATCCGCGCCTTTGCGAGACTGCACTCTCTTTATACGCTTCACAGACCAAAAGAAACCAGCAAGAACTATGCAGGGCGGCCATGGTTTCTTCTCTCAAAGCTGCACAAAACGCCACCTCCATCATCTCCAAGATCTCCCGTCGGAAGTTGTCACCCTATGAAGCCGAAGTCATCGGAGATTGCATCGATAATCTCAATGACTCCGTCGACGAGATCCGTCGAGCTACCACTGCCATTAAAAAATTGAGTCGTTCTAAGGATGTGGATTTTCAGTTGAGTAGTATAAAGACGTGGATGAGCGCAGCAGAGACCGATGTTATCACGTGCACCGACGGGTTGAGCGGCGCGGCGGGGCGGAAGGTGAGGACGAAGGTGAAGAAAGAGGTGAAGAATTGTAGTGTGATTGTGGTAAGGCAGATTAGTAATGCCTTGTCTCTCATCAACAACTTCAACTACAAACACTGA

Protein sequence

MESQILKSSLTLFFFIVLTTFTTSAAASSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRKLSPYEAEVIGDCIDNLNDSVDEIRRATTAIKKLSRSKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVKNCSVIVVRQISNALSLINNFNYKH
Homology
[Close]
BLAST of Bhi01G000901 vs. TAIR10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 101.3 bits (251), Expect = 7.2e-22
Identity = 61/163 (37.42%), Postives = 96/163 (58.90%), Query Frame = 0 

Query: 40  IRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRK-L 99
           I+ +CK T YP LC  +LS+YA+  + + + L   A+  +L  AQ+    +S+++R K L
Sbjct: 39  IQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKGL 98

Query: 100 SPYEAEVIGDCIDNLNDSVDEIRRATTAIKKLSRSKDVD---FQLSSIKTWMSAAETDVI 159
              E E I DC++ +ND+VD + ++   +K    +KD D   + +S+ +TW SAA TD  
Sbjct: 99  KKREVEAIKDCVEEMNDTVDRLTKSVQELKLCGSAKDQDQFAYHMSNAQTWTSAALTDEN 158

Query: 160 TCTDGLSGAAGRKVRTKVKKEVKNCSVIVVRQISNALSLINNF 199
           TC+DG S   GR +  ++K  V+   + V  + SNALSLIN F
Sbjct: 159 TCSDGFS---GRVMDGRIKNSVRARIMNVGHETSNALSLINAF 198

[Close]
BLAST of Bhi01G000901 vs. TAIR10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 93.2 bits (230), Expect = 2.0e-19
Identity = 56/167 (33.53%), Postives = 96/167 (57.49%), Query Frame = 0 

Query: 40  IRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRK-L 99
           I+ +C+ T YP  C  +LS YAS+ K+  ++L   A+  S+  A++A + +S+++  K +
Sbjct: 38  IKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALAVSIARAKSAKTYVSEMTDYKGI 97

Query: 100 SPYEAEVIGDCIDNLNDSVDEIRRATTAIKKLSR---SKDVDFQLSSIKTWMSAAETDVI 159
           +  + E + DC++ + D+VD +  +   +K L      +D  F LS+++TW SAA TD  
Sbjct: 98  TKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGDSGEDFWFCLSNVRTWTSAALTDET 157

Query: 160 TCTDGLSGAAGRKVRTKVKKEVKNCSVIVVRQISNALSLINNFNYKH 203
            C DG  G A   +  ++K  ++   V V  + SNAL+LIN+F  KH
Sbjct: 158 ACMDGFGGKA---MAGELKSLIRTHIVSVAEETSNALALINDFASKH 201

[Close]
BLAST of Bhi01G000901 vs. TAIR10
Match: AT4G12390.1 (pectin methylesterase inhibitor 1)

HSP 1 Score: 90.9 bits (224), Expect = 9.8e-19
Identity = 56/168 (33.33%), Postives = 97/168 (57.74%), Query Frame = 0 

Query: 40  IRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKISR-RKL 99
           I  +C+ T Y  LC   L+ +A + +RN+ +L + A+  +L   Q+ T  + K+++ R++
Sbjct: 40  IVSSCRVTRYQTLCVKCLAAFADKIRRNENQLAQTALAVTLVRVQSTTIYVGKLTKARRI 99

Query: 100 SPYEAEVIGDCIDNLNDSVDEIRRATTAIKKLSRS-KDVD---FQLSSIKTWMSAAETDV 159
              E   + DC++NL D ++ + ++   +K++ RS +D D   ++LS+++TW+SAA TD 
Sbjct: 100 KRREYLAVKDCVENLGDGLEMLAQSMRELKQVGRSGRDRDEFLWRLSNVETWVSAALTDE 159

Query: 160 ITCTDGLSGAAGRKVRTKVKKEVKNCSVIVVRQISNALSLINNFNYKH 203
            TC DG  G     V   VK  ++   V V R  SNAL+L+N F  +H
Sbjct: 160 TTCLDGFDGKVMDGV---VKSAIRRRVVHVARVTSNALALVNRFAARH 204

[Close]
BLAST of Bhi01G000901 vs. TAIR10
Match: AT1G62770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 89.4 bits (220), Expect = 2.8e-18
Identity = 63/171 (36.84%), Postives = 100/171 (58.48%), Query Frame = 0 

Query: 40  IRKACKPTPYPRLCETALSLYASQTK-RNQQELCRAAMVSSLKAAQNATSIISKISRR-- 99
           I  +C+ T YP LC   LS YA++ +  N Q+L + A++ SL  A++ T  ++K+++   
Sbjct: 34  IVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQTALIISLARAKSVTIFVAKLTKETP 93

Query: 100 KLSPYEAEVIGDCIDNLNDSVDEIRRATTAIKKLSR------SKDVDFQLSSIKTWMSAA 159
           K    E   I DCI+ L +SVD   R   ++K+L+R      S+D  +++S+++TW+SAA
Sbjct: 94  KFKRREYLAIKDCIEVLGNSVD---RLAQSVKELARAGHAVASEDFMWKMSNVQTWVSAA 153

Query: 160 ETDVITCTDGLSGAAGRKVRTKVKKEVKNCSVIVVRQISNALSLINNFNYK 202
            TD  TC DG S    R +  KVK+ ++   V V +  SNAL+L+N F  K
Sbjct: 154 LTDETTCLDGFS---ERAMGGKVKRLIRYKVVHVAQVTSNALALVNQFAEK 198

[Close]
BLAST of Bhi01G000901 vs. TAIR10
Match: AT1G14890.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 88.6 bits (218), Expect = 4.8e-18
Identity = 58/164 (35.37%), Postives = 95/164 (57.93%), Query Frame = 0 

Query: 40  IRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRKLS 99
           IR +C  T YP +C T+L+ YAS  + N   L + A+  SL  A+   + +SK+SRR  S
Sbjct: 59  IRTSCNTTLYPDVCYTSLAGYASAVQDNPARLAKLAIGVSLSRAKYTAAYLSKLSRRAAS 118

Query: 100 PYEAEVIGDCIDNLNDSVDEIRRATTAIKKLSRSKDVD----FQLSSIKTWMSAAETDVI 159
                 + DC+ N+ D+VD++R +   +++++  +  D    FQ+S+++TWMSAA TD  
Sbjct: 119 ----AAVHDCVSNVGDAVDQMRGSLRQLREMNHRRPGDPAFRFQMSNVQTWMSAALTDEE 178

Query: 160 TCTDGLS-GAAGRKVRTKVKKEVKNCSVIVVRQISNALSLINNF 199
           TCTDG++      + +T +   V +    V R  SNAL+L+N +
Sbjct: 179 TCTDGVTEEMEDGETKTAICDRVAD----VKRFTSNALALVNTY 214

[Close]
BLAST of Bhi01G000901 vs. Swiss-Prot
Match: sp|P17407|21KD_DAUCA (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.3e-23
Identity = 66/171 (38.60%), Postives = 100/171 (58.48%), Query Frame = 0 

Query: 35  PVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKIS 94
           P    I+ +C  T YP +CE +LS YA   + N QEL   A+  SL   Q A + + +++
Sbjct: 24  PANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQVSLTRTQQAQTFMKRLN 83

Query: 95  RRK-LSPYEAEVIGDCIDNLNDSVDEIRRATTAIKKLSRSK--DVDFQLSSIKTWMSAAE 154
           + K L   +   I DC++ + DS+D + R+   +K LS +K  D  F++S+++TW+SAA 
Sbjct: 84  KFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGNDFTFRMSNVETWVSAAL 143

Query: 155 TDVITCTDGLSGAAGRKVRTKVKKEVKNCSVIVVRQISNALSLINNFNYKH 203
           TD  TC DG    AG+ +  K+K+ V+   V V R  SNAL+L+NNF  KH
Sbjct: 144 TDETTCMDGF---AGKGMDGKIKESVRAQVVAVARVTSNALALVNNFAAKH 191

[Close]
BLAST of Bhi01G000901 vs. Swiss-Prot
Match: sp|Q9SB37|PMEI7_ARATH (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 3.5e-18
Identity = 56/167 (33.53%), Postives = 96/167 (57.49%), Query Frame = 0 

Query: 40  IRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRK-L 99
           I+ +C+ T YP  C  +LS YAS+ K+  ++L   A+  S+  A++A + +S+++  K +
Sbjct: 38  IKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALAVSIARAKSAKTYVSEMTDYKGI 97

Query: 100 SPYEAEVIGDCIDNLNDSVDEIRRATTAIKKLSR---SKDVDFQLSSIKTWMSAAETDVI 159
           +  + E + DC++ + D+VD +  +   +K L      +D  F LS+++TW SAA TD  
Sbjct: 98  TKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGDSGEDFWFCLSNVRTWTSAALTDET 157

Query: 160 TCTDGLSGAAGRKVRTKVKKEVKNCSVIVVRQISNALSLINNFNYKH 203
            C DG  G A   +  ++K  ++   V V  + SNAL+LIN+F  KH
Sbjct: 158 ACMDGFGGKA---MAGELKSLIRTHIVSVAEETSNALALINDFASKH 201

[Close]
BLAST of Bhi01G000901 vs. Swiss-Prot
Match: sp|Q9SI72|PMEI9_ARATH (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 5.1e-17
Identity = 63/171 (36.84%), Postives = 100/171 (58.48%), Query Frame = 0 

Query: 40  IRKACKPTPYPRLCETALSLYASQTK-RNQQELCRAAMVSSLKAAQNATSIISKISRR-- 99
           I  +C+ T YP LC   LS YA++ +  N Q+L + A++ SL  A++ T  ++K+++   
Sbjct: 34  IVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQTALIISLARAKSVTIFVAKLTKETP 93

Query: 100 KLSPYEAEVIGDCIDNLNDSVDEIRRATTAIKKLSR------SKDVDFQLSSIKTWMSAA 159
           K    E   I DCI+ L +SVD   R   ++K+L+R      S+D  +++S+++TW+SAA
Sbjct: 94  KFKRREYLAIKDCIEVLGNSVD---RLAQSVKELARAGHAVASEDFMWKMSNVQTWVSAA 153

Query: 160 ETDVITCTDGLSGAAGRKVRTKVKKEVKNCSVIVVRQISNALSLINNFNYK 202
            TD  TC DG S    R +  KVK+ ++   V V +  SNAL+L+N F  K
Sbjct: 154 LTDETTCLDGFS---ERAMGGKVKRLIRYKVVHVAQVTSNALALVNQFAEK 198

[Close]
BLAST of Bhi01G000901 vs. Swiss-Prot
Match: sp|Q84WE4|PMEI3_ARATH (Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 3.7e-15
Identity = 56/168 (33.33%), Postives = 88/168 (52.38%), Query Frame = 0 

Query: 40  IRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATS----IISKISR 99
           +  +C+   YP LC   LS Y+  T  N+++L +AA+  SL  AQ+A      +   + +
Sbjct: 34  VHSSCEHASYPSLCVRTLSSYSGPTITNRRDLAQAAIKISLSHAQSAAKKLAVVRDSVGK 93

Query: 100 RKLSPYEAEVIGDCIDNLNDSVDEIRRATTAIKKL----SRSKDVDFQLSSIKTWMSAAE 159
           +K    E   + DC++ + DSVDE+ R    +K L      +K+  +Q+S+ +TW SAA 
Sbjct: 94  KK---QEKAALVDCVEMIGDSVDELSRTLGVLKHLRVSGGSAKEFRWQMSNAQTWASAAL 153

Query: 160 TDVITCTDGLSGAAGRKVRTKVKKEVKNCSVIVVRQISNALSLINNFN 200
           TD  TC DG  G    +++T    EVK     V R  SNAL ++N  +
Sbjct: 154 TDDDTCLDGFQGMDDGEIKT----EVKQWMTKVARVTSNALYMVNQLD 194

[Close]
BLAST of Bhi01G000901 vs. Swiss-Prot
Match: sp|Q9STY5|PMI11_ARATH (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 8.2e-15
Identity = 54/167 (32.34%), Postives = 92/167 (55.09%), Query Frame = 0 

Query: 40  IRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKISR-RKL 99
           I+ +CK T YP +C  +L+ YA+  + + + L   A+  ++  AQ+    + ++ R   L
Sbjct: 39  IQASCKATTYPTVCVNSLTGYANSIQTSPRRLAETALNVTVTQAQSTKVFVWRLGRFTSL 98

Query: 100 SPYEAEVIGDCIDNLNDSVDEIRRATTAIKKLSRSKDVD---FQLSSIKTWMSAAETDVI 159
              E + + DCI+ ++D+VD +  +   +K    +K  D   F +S+ +TW SAA T+  
Sbjct: 99  KKREIQAVKDCIEEIHDAVDRLTMSIHEVKMCGSAKGRDQFWFHMSNAQTWTSAALTNAN 158

Query: 160 TCTDGLSGAAGRKVRTKVKKEVKNCSVIVVRQISNALSLINNFNYKH 203
           TC+DG    AGR +  +VK  V+   + + R  SNAL+LIN F  K+
Sbjct: 159 TCSDGF---AGRVMDGRVKNSVRARILNLGRGTSNALALINAFAKKY 202

[Close]
BLAST of Bhi01G000901 vs. TrEMBL
Match: tr|A0A0A0L671|A0A0A0L671_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G176250 PE=4 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 1.4e-75
Identity = 181/203 (89.16%), Postives = 191/203 (94.09%), Query Frame = 0 

Query: 1   MESQILKSS--XXXXXXXXXXXXXXXXXXXXXXXVRPVQPHIRKACKPTPYPRLCETALS 60
           MESQILKSS  XXXXXXXXXXXXXXXXXXXXXXXVRPVQPHIRKACKPTPYPRLCETALS
Sbjct: 1   MESQILKSSLXXXXXXXXXXXXXXXXXXXXXXXXVRPVQPHIRKACKPTPYPRLCETALS 60

Query: 61  LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRKLSPYEAEVIGDCIDNLNDSVD 120
           LYASQTKRNQQELCRAAMVSSLKAAQNATSIISK+SRRK+S YEAEVIGDCIDNL DSVD
Sbjct: 61  LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSVD 120

Query: 121 EIRRATTAIKKLSRSKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVK 180
           E+RRA+TAIK LSRSKDVDFQL+SIKTWMSAA+TDVITCTDGLSG +G KV   +KK+VK
Sbjct: 121 ELRRASTAIKSLSRSKDVDFQLNSIKTWMSAAQTDVITCTDGLSGGSGWKVSKMLKKDVK 180

Query: 181 NCSVIVVRQISNALSLINNFNYK 202
           NCS+ VVRQISNAL LINNFNYK
Sbjct: 181 NCSINVVRQISNALFLINNFNYK 203

[Close]
BLAST of Bhi01G000901 vs. TrEMBL
Match: tr|A0A1S3AXZ9|A0A1S3AXZ9_CUCME (21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103483989 PE=4 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 5.2e-75
Identity = 180/202 (89.11%), Postives = 190/202 (94.06%), Query Frame = 0 

Query: 1   MESQILKSS-XXXXXXXXXXXXXXXXXXXXXXXVRPVQPHIRKACKPTPYPRLCETALSL 60
           MESQILKSS XXXXXXXXXXXXXXXXXXXXXXXVRPV+PHIRKACKPTPYPRLCETALSL
Sbjct: 1   MESQILKSSLXXXXXXXXXXXXXXXXXXXXXXXVRPVKPHIRKACKPTPYPRLCETALSL 60

Query: 61  YASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRKLSPYEAEVIGDCIDNLNDSVDE 120
           YASQTKRNQQELCRAAMVSSLKAAQNATSIISK+SRRK+S YE EVIGDCID+L DSVDE
Sbjct: 61  YASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYETEVIGDCIDDLKDSVDE 120

Query: 121 IRRATTAIKKLSRSKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVKN 180
           +RRA+TAIK LSRSKDVDFQL+SIKTW SAA TD+ITCTDGLSG +G KV  KVKKEVKN
Sbjct: 121 LRRASTAIKSLSRSKDVDFQLNSIKTWTSAALTDIITCTDGLSGGSGWKVNKKVKKEVKN 180

Query: 181 CSVIVVRQISNALSLINNFNYK 202
           CS+ VVRQISNALSLINNFNYK
Sbjct: 181 CSINVVRQISNALSLINNFNYK 202

[Close]
BLAST of Bhi01G000901 vs. TrEMBL
Match: tr|B9R9Q8|B9R9Q8_RICCO (21 kDa protein, putative OS=Ricinus communis OX=3988 GN=RCOM_1500020 PE=4 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 3.1e-27
Identity = 73/163 (44.79%), Postives = 105/163 (64.42%), Query Frame = 0 

Query: 39  HIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRK- 98
           +++ AC  T YP++C   LS Y S  + N  +LC AA+  +LKAA N ++++  +S++K 
Sbjct: 47  YLKTACNSTTYPKICYNTLSPYTSTIQTNDLKLCNAALTITLKAASNTSAMVKSLSKQKG 106

Query: 99  LSPYEAEVIGDCIDNLNDSVDEIRRATTAIKKLSRSKDVDFQLSSIKTWMSAAETDVITC 158
           LS  E  VI DC   + DSVDE++++  A+K L  S D++FQ+ +IKTW+SAA TD  TC
Sbjct: 107 LSKGEVAVIKDCQYEIEDSVDELKQSLKALKNLKGSADMEFQIDNIKTWISAAITDENTC 166

Query: 159 TDGLSGAAGRKVRTKVKKEVKNCSVIVVRQISNALSLINNFNY 201
           TDG     G KV +KVK ++K   V V R  SNAL+LIN  +Y
Sbjct: 167 TDGFE---GMKVSSKVKSKIKKSIVNVNRLTSNALALINKLSY 206

[Close]
BLAST of Bhi01G000901 vs. TrEMBL
Match: tr|A0A2I4HWD8|A0A2I4HWD8_9ROSI (21 kDa protein-like OS=Juglans regia OX=51240 GN=LOC109022115 PE=4 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 2.0e-26
Identity = 77/165 (46.67%), Postives = 108/165 (65.45%), Query Frame = 0 

Query: 39  HIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISK---ISR 98
           +I  +C  T YP+LC T+LS YAS+ K N ++LC+ ++   L+AAQNA+SI+SK     +
Sbjct: 45  YIITSCNSTTYPQLCYTSLSPYASKIKANPKKLCKTSLSVGLEAAQNASSIVSKQLIKQK 104

Query: 99  RKLSPYEAEVIGDCIDNLNDSVDEIRRATTAIKKLSRSKDVDFQLSSIKTWMSAAETDVI 158
             L+  E  VI DCI N+ +S+DE+R +  A+ KLS   D +FQ+S+IKTW+SAA TD  
Sbjct: 105 GSLTSSETAVIKDCIVNIKNSIDELRESLDAMDKLS-GWDKEFQMSNIKTWVSAALTDEN 164

Query: 159 TCTDGLSGAAGRKVRTKVKKEVKNCSVIVVRQISNALSLINNFNY 201
           TC DG     G+++  KVK +V+   + V R  SNAL LINN NY
Sbjct: 165 TCLDGFE--EGQEISEKVKNKVRKRILEVARLTSNALYLINNLNY 206

[Close]
BLAST of Bhi01G000901 vs. TrEMBL
Match: tr|A0A2R6PLX9|A0A2R6PLX9_ACTCH (Pectinesterase inhibitor domain protein OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc27963 PE=4 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 3.4e-26
Identity = 75/162 (46.30%), Postives = 101/162 (62.35%), Query Frame = 0 

Query: 40  IRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRK-L 99
           IR +C  T YP LC   LS YA+  K N   LC AA+  SL  A+NA+S +SK SR+K +
Sbjct: 36  IRTSCANTTYPSLCTKTLSPYAAIVKSNPWRLCYAALSVSLDTARNASSTVSKFSRQKGI 95

Query: 100 SPYEAEVIGDCIDNLNDSVDEIRRATTAIKKLSRSKDVDFQLSSIKTWMSAAETDVITCT 159
               A  I DCI+N+ DSVDE++++  A++ + R+ +   Q+++IKTW SAA TD  TCT
Sbjct: 96  RSRAAAAIKDCIENIGDSVDELKQSLRAMESIGRNANKQLQMANIKTWTSAAITDGDTCT 155

Query: 160 DGLSGAAGRKVRTKVKKEVKNCSVIVVRQISNALSLINNFNY 201
           DGL   AG KV   VK  +++     +R  SNALSLIN  NY
Sbjct: 156 DGL---AGTKVNAGVKSNIRSVVKSFIRVTSNALSLINRLNY 194

[Close]
BLAST of Bhi01G000901 vs. NCBI nr
Match: XP_004148137.1 (PREDICTED: 21 kDa protein-like [Cucumis sativus] >KGN57278.1 hypothetical protein Csa_3G176250 [Cucumis sativus])

HSP 1 Score: 291.6 bits (745), Expect = 2.1e-75
Identity = 181/203 (89.16%), Postives = 191/203 (94.09%), Query Frame = 0 

Query: 1   MESQILKSS--XXXXXXXXXXXXXXXXXXXXXXXVRPVQPHIRKACKPTPYPRLCETALS 60
           MESQILKSS  XXXXXXXXXXXXXXXXXXXXXXXVRPVQPHIRKACKPTPYPRLCETALS
Sbjct: 1   MESQILKSSLXXXXXXXXXXXXXXXXXXXXXXXXVRPVQPHIRKACKPTPYPRLCETALS 60

Query: 61  LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRKLSPYEAEVIGDCIDNLNDSVD 120
           LYASQTKRNQQELCRAAMVSSLKAAQNATSIISK+SRRK+S YEAEVIGDCIDNL DSVD
Sbjct: 61  LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSVD 120

Query: 121 EIRRATTAIKKLSRSKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVK 180
           E+RRA+TAIK LSRSKDVDFQL+SIKTWMSAA+TDVITCTDGLSG +G KV   +KK+VK
Sbjct: 121 ELRRASTAIKSLSRSKDVDFQLNSIKTWMSAAQTDVITCTDGLSGGSGWKVSKMLKKDVK 180

Query: 181 NCSVIVVRQISNALSLINNFNYK 202
           NCS+ VVRQISNAL LINNFNYK
Sbjct: 181 NCSINVVRQISNALFLINNFNYK 203

[Close]
BLAST of Bhi01G000901 vs. NCBI nr
Match: XP_008439099.1 (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 289.7 bits (740), Expect = 7.9e-75
Identity = 180/202 (89.11%), Postives = 190/202 (94.06%), Query Frame = 0 

Query: 1   MESQILKSS-XXXXXXXXXXXXXXXXXXXXXXXVRPVQPHIRKACKPTPYPRLCETALSL 60
           MESQILKSS XXXXXXXXXXXXXXXXXXXXXXXVRPV+PHIRKACKPTPYPRLCETALSL
Sbjct: 1   MESQILKSSLXXXXXXXXXXXXXXXXXXXXXXXVRPVKPHIRKACKPTPYPRLCETALSL 60

Query: 61  YASQTKRNQQELCRAAMVSSLKAAQNATSIISKISRRKLSPYEAEVIGDCIDNLNDSVDE 120
           YASQTKRNQQELCRAAMVSSLKAAQNATSIISK+SRRK+S YE EVIGDCID+L DSVDE
Sbjct: 61  YASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYETEVIGDCIDDLKDSVDE 120

Query: 121 IRRATTAIKKLSRSKDVDFQLSSIKTWMSAAETDVITCTDGLSGAAGRKVRTKVKKEVKN 180
           +RRA+TAIK LSRSKDVDFQL+SIKTW SAA TD+ITCTDGLSG +G KV  KVKKEVKN
Sbjct: 121 LRRASTAIKSLSRSKDVDFQLNSIKTWTSAALTDIITCTDGLSGGSGWKVNKKVKKEVKN 180

Query: 181 CSVIVVRQISNALSLINNFNYK 202
           CS+ VVRQISNALSLINNFNYK
Sbjct: 181 CSINVVRQISNALSLINNFNYK 202

[Close]
BLAST of Bhi01G000901 vs. NCBI nr
Match: XP_023553135.1 (21 kDa protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 271.2 bits (692), Expect = 2.9e-69
Identity = 137/169 (81.07%), Postives = 155/169 (91.72%), Query Frame = 0 

Query: 33  VRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISK 92
           ++PV+PHIRKAC+PTPYPRLCETALSLY+SQTKR QQELCRAA+VSSLKAAQNATSIISK
Sbjct: 35  IQPVKPHIRKACRPTPYPRLCETALSLYSSQTKRTQQELCRAAVVSSLKAAQNATSIISK 94

Query: 93  ISRRKLSPYEAEVIGDCIDNLNDSVDEIRRATTAIKKLSRSKDVDFQLSSIKTWMSAAET 152
           +SRRK+SPYEAEVIGDCIDNL DSV E+RRATTAIK L+R+ DV+FQLSSIKTW+SA ET
Sbjct: 95  LSRRKVSPYEAEVIGDCIDNLKDSVYELRRATTAIKSLNRANDVEFQLSSIKTWLSAGET 154

Query: 153 DVITCTDGLSGAAGRKVRTKVKKEVKNCSVIVVRQISNALSLINNFNYK 202
           D++TCTDGLSG AG KVR+ VKKEVK CSV V+RQ SNALSLI+N  YK
Sbjct: 155 DIMTCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKYK 203

[Close]
BLAST of Bhi01G000901 vs. NCBI nr
Match: XP_022985512.1 (pectinesterase inhibitor 4-like [Cucurbita maxima])

HSP 1 Score: 266.2 bits (679), Expect = 9.3e-68
Identity = 134/169 (79.29%), Postives = 154/169 (91.12%), Query Frame = 0 

Query: 33  VRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISK 92
           ++PV+PHIRKAC+PTPYPRLCETALSLY+SQTKR QQELCRAA+VSSLKAAQNATSIISK
Sbjct: 28  IQPVKPHIRKACRPTPYPRLCETALSLYSSQTKRTQQELCRAAVVSSLKAAQNATSIISK 87

Query: 93  ISRRKLSPYEAEVIGDCIDNLNDSVDEIRRATTAIKKLSRSKDVDFQLSSIKTWMSAAET 152
           +S RK+SPYEAEVIGDC DNL DS+ E+RRATTAIK L+R+ DV+FQLSSIKTW+SAAET
Sbjct: 88  LSHRKVSPYEAEVIGDCTDNLKDSIYELRRATTAIKSLNRADDVEFQLSSIKTWLSAAET 147

Query: 153 DVITCTDGLSGAAGRKVRTKVKKEVKNCSVIVVRQISNALSLINNFNYK 202
           +++TCTDGLSG AG KVR+ VKKEVK CSV V+RQ SNALSLI+N  YK
Sbjct: 148 NIMTCTDGLSGGAGWKVRSMVKKEVKKCSVNVIRQTSNALSLISNLKYK 196

[Close]
BLAST of Bhi01G000901 vs. NCBI nr
Match: XP_022922937.1 (pectinesterase inhibitor 4-like [Cucurbita moschata])

HSP 1 Score: 263.8 bits (673), Expect = 4.6e-67
Identity = 134/167 (80.24%), Postives = 152/167 (91.02%), Query Frame = 0 

Query: 35  PVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKIS 94
           PV+PHI+KAC+PTPYPRLCETALSLY+SQTK  QQELCRAA+VSSLKAAQNATSIISK+S
Sbjct: 37  PVKPHIKKACRPTPYPRLCETALSLYSSQTKTTQQELCRAAVVSSLKAAQNATSIISKLS 96

Query: 95  RRKLSPYEAEVIGDCIDNLNDSVDEIRRATTAIKKLSRSKDVDFQLSSIKTWMSAAETDV 154
           RRK+SPYEAEVIGDCIDNL DSV E+RRATTAIK L+R+ +V+FQLSSIKTW+SAA TD+
Sbjct: 97  RRKVSPYEAEVIGDCIDNLKDSVYELRRATTAIKSLNRANNVEFQLSSIKTWLSAAGTDI 156

Query: 155 ITCTDGLSGAAGRKVRTKVKKEVKNCSVIVVRQISNALSLINNFNYK 202
           +TCTDGLSG AG KVR+ VKKEVK CSV V+RQ SNALSLI+N  YK
Sbjct: 157 VTCTDGLSGGAGWKVRSTVKKEVKKCSVNVIRQTSNALSLISNLKYK 203

The following BLAST results are available for this feature:
BLAST of Bhi01G000901 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G62350.17.2e-2237.42Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT4G25260.12.0e-1933.53Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT4G12390.19.8e-1933.33pectin methylesterase inhibitor 1[more]
AT1G62770.12.8e-1836.84Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G14890.14.8e-1835.37Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
BLAST of Bhi01G000901 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|P17407|21KD_DAUCA1.3e-2338.6021 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1[more]
sp|Q9SB37|PMEI7_ARATH3.5e-1833.53Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1[more]
sp|Q9SI72|PMEI9_ARATH5.1e-1736.84Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1[more]
sp|Q84WE4|PMEI3_ARATH3.7e-1533.33Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1[more]
sp|Q9STY5|PMI11_ARATH8.2e-1532.34Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1[more]
BLAST of Bhi01G000901 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0L671|A0A0A0L671_CUCSA1.4e-7589.16Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G176250 PE=4 SV=1[more]
tr|A0A1S3AXZ9|A0A1S3AXZ9_CUCME5.2e-7589.1121 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103483989 PE=4 SV=1[more]
tr|B9R9Q8|B9R9Q8_RICCO3.1e-2744.7921 kDa protein, putative OS=Ricinus communis OX=3988 GN=RCOM_1500020 PE=4 SV=1[more]
tr|A0A2I4HWD8|A0A2I4HWD8_9ROSI2.0e-2646.6721 kDa protein-like OS=Juglans regia OX=51240 GN=LOC109022115 PE=4 SV=1[more]
tr|A0A2R6PLX9|A0A2R6PLX9_ACTCH3.4e-2646.30Pectinesterase inhibitor domain protein OS=Actinidia chinensis var. chinensis OX... [more]
BLAST of Bhi01G000901 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004148137.12.1e-7589.16PREDICTED: 21 kDa protein-like [Cucumis sativus] >KGN57278.1 hypothetical protei... [more]
XP_008439099.17.9e-7589.11PREDICTED: 21 kDa protein-like [Cucumis melo][more]
XP_023553135.12.9e-6981.0721 kDa protein-like [Cucurbita pepo subsp. pepo][more]
XP_022985512.19.3e-6879.29pectinesterase inhibitor 4-like [Cucurbita maxima][more]
XP_022922937.14.6e-6780.24pectinesterase inhibitor 4-like [Cucurbita moschata][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR035513Invertase/methylesterase_inhib
IPR006501Pectinesterase_inhib_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi01M000901Bhi01M000901mRNA


InterPro
Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 35..194
e-value: 2.3E-35
score: 133.5
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 40..194
e-value: 3.2E-28
score: 98.9
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 7..197
e-value: 9.6E-31
score: 104.4
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3DG3DSA:1.20.140.40coord: 37..193
e-value: 3.9E-20
score: 74.2
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILYSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 38..198
NoneNo IPR availablePANTHERPTHR31080FAMILY NOT NAMEDcoord: 10..198
NoneNo IPR availablePANTHERPTHR31080:SF15INVERTASEcoord: 10..198
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 43..198
e-value: 6.96848E-37
score: 126.784