Lsi05G013180.1 (mRNA) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G013180.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	chr05 : 20940187 .. 20942571 (-)
Sequence length	941

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGTTAAACTAAACACATGTATTCCATTTTTCATGTTTAAATTTCTATGTTAATGTCTTCATGACAAATTATAAAAACTTATGAAAAGATAACTTCTCATGAACCGATTTCTTCAAATATTAGAAAATGTTAATGTCTATCTTTAAAGCCATGTTTTTATGGGAAATTGACATTATCTTTAAAAAAAAAAAAAAAATCCATTAAGAAACTAAAACCATAAACCACCAACAACCATAACCCACATCATCCTCTTTTCAAAAGTAAGAGGAACAATATAAGTTAGAAAGACATCTTTTCAGACTCGGGAGAACCCAGAAGAGAAGGTTAAATATGCTAAATTGTGGGCAACTTTGTTCTCCACTACAAGAAATAACATATACGATAGCTAACGGAAAAAAAAAAACTATAATAGACCTTTTATAGCCTTTTTCGAAAGTCCTGACAACCTATGTTATTAAAAGTCTGAGACGTTCCACAACATCAATTTCAACATTCACTAGAAGATTTTAGTCATGCTATTGACAAACGATGATAAATTCGAGGTAGTTAAGTCGTTTAAAGATATTTCATGCATCTAAGAATGTAAAATTTCTAGTCACAAATGTGAATACCTTCTCAAATTTTTTCTTTCAAACATGTGGCATGTTTTCCGCACCTATAAATATGGTAAAAATCTCCCTTATCTTCATTATTATAACAAAGACAAGCCTAATAGATTCTTTAGAGAGAGACAGAGGGAGATGGAATCTCAAATCCTAAAATCCTTACAAACCCTTTTCTTCTTTATTATTTTAACTACATTCACCACCTCCGCCGTGGCCTCTTCTTCCTCCTCCACCGTCCGGCCAGTACAACCACACATCAGAAAGGCCTGCAAGCCGACGCCATATCCACGCCTTTGCGAGACTGCACTCTCCTTATACGCTTCACAAACCAAAAGAAACCAGCAAGAGCTCTGCAGGGCGGCCATGGCTTCTTCTCTCAAAGCTGCACAAAACGCCACCTCCATCATCTCCAAGCTCTCCCGTCGGAAGGTATCGCCCTATGAAGCCGAAGTCATCGGAGATTGCATCGATAATCTCAATGACTCTGTCGATGAGCTCCGTCGAGCTGCCGCTGCCATCAAAAGCTTGAGTCGTTCTAACGACGTGGAGTTTCAAGTTGTTAAAAACTACTACTTAATCTCATGGTTATTTAAGTTAAAAAACACTTTTAATCTCAAAACTTTCATTAAAGTAACAGTTTATAATTTAATCTTTATACTCTCAAATTTGTAACAATTTAATCCTCTGAACTTTAATAATTAACGTTTTAGTCCTTTTACTTTACTATTTATAATGATTTAGCCTCTAACGTAAAAAAATTCATCAAAATTAAGTGTTAATTTTTATTACATAATGACTCGAGTCTTTTGTACTAATATTTTTTACCATAGGAACTAAATCACTATAAATTGTAAAGTACATAGACTAAATTGTTATTTATTAAAGTTGAGGGGCTAAATTATTACAAATTTAAAAGTAGAAAAACTAAATTGTTATCAGCTAAAATTTAAAGATCAAATTGTTATTTTCATGAAATTTCAAGGACCAAAAGTATTTTTTAATTTAAAAATTTTACGTATACGTGTGTTGTATGTGAATAAAAATTTGAACTTACAATATTTGAAAAAAAAATCGAGATATGTTAATCGCTAATATTTTCTTTTGATAAATACTCTTTAAAAAATCATGGAAATCAAAATAACTTTATATCTAACCTTTTAAGTAAAGTTAAGAATTCTTGTTGTTTAAGATTATATTCACATGATGTGGTCTAATTCAAATATTACTTTCTAAGTCTAAAGTTTTAATCTAGATTAGGTTAAACTTAATCTTTTATTCATATTCTTAACGCAGTTGAGTAGTATAAAAACATGGGCGAGCGCAGCACTGACCGATGTTATCACGTGCACCGACGGGTTGAGCGGCGGCTCGGGGTGGAAGGTGAGATCGAAGTTGAAGAAAGAAGTCAAGAATTGTAGTGCGAATGTAGTAAGGCAGATTAGTAATGCCTTGTTTCTCATCAACAACTTCAACTACAAATAGTGTGAGCTCTGAGTAGTTTTTGAAAGAAACTTCCATGGCCACACCCATTAAACTCACTTGGAACTTGTTCCAAATGAATTCATTTTCCTTTGTAGTTCCACCTTCCATTAAAAAAAAGTATAAAATATGTTTCTTTTTGTGTGTTTTTTTTTTATCATGTGAATTCTTTCCTTAATTTCTCAATGTCTTTTTGGGTATATACATAATTACAATTTCATCACTTTAAATAGTTATACTTCTAAATTTTACATTTACATTTTTTTAATAAATAATACTTCAATGCATTTCAATCTTCA

mRNA sequence

ATGCACAAGCCTAATAGATTCTTTAGAGAGAGACAGAGGGAGATGGAATCTCAAATCCTAAAATCCTTACAAACCCTTTTCTTCTTTATTATTTTAACTACATTCACCACCTCCGCCGTGGCCTCTTCTTCCTCCTCCACCGTCCGGCCAGTACAACCACACATCAGAAAGGCCTGCAAGCCGACGCCATATCCACGCCTTTGCGAGACTGCACTCTCCTTATACGCTTCACAAACCAAAAGAAACCAGCAAGAGCTCTGCAGGGCGGCCATGGCTTCTTCTCTCAAAGCTGCACAAAACGCCACCTCCATCATCTCCAAGCTCTCCCGTCGGAAGGTATCGCCCTATGAAGCCGAAGTCATCGGAGATTGCATCGATAATCTCAATGACTCTGTCGATGAGCTCCGTCGAGCTGCCGCTGCCATCAAAAGCTTGAGTCTAAAGTTAAGAATTCTTTTGAGTAGTATAAAAACATGGGCGAGCGCAGCACTGACCGATGTTATCACGTGCACCGACGGGTTGAGCGGCGGCTCGGGGTGGAAGGTGAGATCGAAGTTGAAGAAAGAAGTCAAGAATTGTAGTGCGAATGTAGTAAGGCAGATTAGTAATGCCTTGTTTCTCATCAACAACTTCAACTACAAATAGTGTGAGCTCTGAGTAGTTTTTGAAAGAAACTTCCATGGCCACACCCATTAAACTCACTTGGAACTTGTTCCAAATGAATTCATTTTCCTTTGTAGTTCCACCTTCCATTAAAAAAAAGTATAAAATATGTTTCTTTTTGTGTGTTTTTTTTTTATCATGTGAATTCTTTCCTTAATTTCTCAATGTCTTTTTGGGTATATACATAATTACAATTTCATCACTTTAAATAGTTATACTTCTAAATTTTACATTTACATTTTTTTAATAAATAATACTTCAATGCATTTCAATCTTCA

Coding sequence (CDS)

ATGCACAAGCCTAATAGATTCTTTAGAGAGAGACAGAGGGAGATGGAATCTCAAATCCTAAAATCCTTACAAACCCTTTTCTTCTTTATTATTTTAACTACATTCACCACCTCCGCCGTGGCCTCTTCTTCCTCCTCCACCGTCCGGCCAGTACAACCACACATCAGAAAGGCCTGCAAGCCGACGCCATATCCACGCCTTTGCGAGACTGCACTCTCCTTATACGCTTCACAAACCAAAAGAAACCAGCAAGAGCTCTGCAGGGCGGCCATGGCTTCTTCTCTCAAAGCTGCACAAAACGCCACCTCCATCATCTCCAAGCTCTCCCGTCGGAAGGTATCGCCCTATGAAGCCGAAGTCATCGGAGATTGCATCGATAATCTCAATGACTCTGTCGATGAGCTCCGTCGAGCTGCCGCTGCCATCAAAAGCTTGAGTCTAAAGTTAAGAATTCTTTTGAGTAGTATAAAAACATGGGCGAGCGCAGCACTGACCGATGTTATCACGTGCACCGACGGGTTGAGCGGCGGCTCGGGGTGGAAGGTGAGATCGAAGTTGAAGAAAGAAGTCAAGAATTGTAGTGCGAATGTAGTAAGGCAGATTAGTAATGCCTTGTTTCTCATCAACAACTTCAACTACAAATAG

Protein sequence

MHKPNRFFRERQREMESQILKSLQTLFFFIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMASSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLNDSVDELRRAAAAIKSLSLKLRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEVKNCSANVVRQISNALFLINNFNYK

Homology

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BLAST of Lsi05G013180.1 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.8e-18
Identity = 67/198 (33.84%), Postives = 101/198 (51.01%), Query Frame = 1


Query: 22  SLQTLFFFIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKR 81
           S  TL F  +L     ++ A        P    I+ +C  T YP +CE +LS YA   + 
Sbjct: 4   SKSTLVFSALLVILAAASAA--------PANQFIKTSCTLTTYPAVCEQSLSAYAKTIQN 63

Query: 82  NQQELCRAAMASSLKAAQNATSIISKLSRRK-VSPYEAEVIGDCIDNLNDSVDELRRAAA 141
           N QEL   A+  SL   Q A + + +L++ K +   +   I DC++ + DS+D + R+  
Sbjct: 64  NPQELASTALQVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCD 123

Query: 142 AIKSLS----LKLRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEVKNCSAN 201
            +K+LS          +S+++TW SAALTD  TC DG +G     +  K+K+ V+     
Sbjct: 124 EMKNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKG---MDGKIKESVRAQVVA 183

Query: 202 VVRQISNALFLINNFNYK 215
           V R  SNAL L+NNF  K
Sbjct: 184 VARVTSNALALVNNFAAK 190

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BLAST of Lsi05G013180.1 vs. Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 56.6 bits (135), Expect = 3.8e-07
Identity = 53/208 (25.48%), Postives = 100/208 (48.08%), Query Frame = 1


Query: 8   FRERQREMESQILKSLQTLFFFIILTTFTTSAVASSSSSTVRPVQPHIRKA-CKPTPYPR 67
           F+  ++ + S    +L  L   + +   TT+   +   +T+      I K+ C  T YP 
Sbjct: 14  FKNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPE 73

Query: 68  LCETALSLYASQTKRNQQELCRAAMASSLKAAQNATSIISKL--SRRKVSPYEAEVIGDC 127
           LC +A++    +   +Q+E+  A++  + KA ++    + KL   R+ ++P E   + DC
Sbjct: 74  LCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDC 133

Query: 128 IDNLNDSVDELRRAAAAIKSL--SLKLRILLSSIKTWASAALTDVITCTDGLSGGSGWKV 187
           ++ +++++DEL  A   +        LR     +KT  S+A+T+  TC DG S     + 
Sbjct: 134 LETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADR- 193

Query: 188 RSKLKKEVKNCSANVVRQISNALFLINN 211
             K++K +     +V    SNAL +I N
Sbjct: 194 --KVRKALLKGQVHVEHMCSNALAMIKN 218

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BLAST of Lsi05G013180.1 vs. Swiss-Prot
Match: PME61_ARATH (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana GN=PME61 PE=2 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.5e-06
Identity = 46/161 (28.57%), Postives = 77/161 (47.83%), Query Frame = 1


Query: 47  TVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAAMASSLKAAQNATSIIS 106
           T +P Q  I + C  + YP LC   L  +      ++ EL   +  ++L+    A    S
Sbjct: 68  TRKPTQA-ISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSS 127

Query: 107 KLSRRKVSPYEAEVIGDCIDNLNDSVDELRRAAAAIKSLSLKLRILLSSIKTWASAALTD 166
            ++  ++ P        C++ L+DSVD L RA +++  +S       S + TW S+A+T+
Sbjct: 128 TITYTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDES--HSDVMTWLSSAMTN 187

Query: 167 VITCTDGLS--GGSGWKVRSKLKKEVKNCSANVVRQISNAL 206
             TCTDG     G G +V+ ++   VK+ S      +SN L
Sbjct: 188 HDTCTDGFDEIEGQGGEVKDQVIGAVKDLS----EMVSNCL 221

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BLAST of Lsi05G013180.1 vs. TrEMBL
Match: A0A0A0L671_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176250 PE=4 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 3.3e-82
Identity = 176/204 (86.27%), Postives = 181/204 (88.73%), Query Frame = 1


Query: 15  MESQILKSLQTL--FFFIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETAL 74
           MESQILKS  TL  FFFIILTTFT SAV SSSSSTVRPVQPHIRKACKPTPYPRLCETAL
Sbjct: 1   MESQILKSSLTLLIFFFIILTTFTPSAV-SSSSSTVRPVQPHIRKACKPTPYPRLCETAL 60

Query: 75  SLYASQTKRNQQELCRAAMASSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLNDSV 134
           SLYASQTKRNQQELCRAAM SSLKAAQNATSIISKLSRRK+S YEAEVIGDCIDNL DSV
Sbjct: 61  SLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSV 120

Query: 135 DELRRAAAAIKSLSLK--LRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEV 194
           DELRRA+ AIKSLS    +   L+SIKTW SAA TDVITCTDGLSGGSGWKV   LKK+V
Sbjct: 121 DELRRASTAIKSLSRSKDVDFQLNSIKTWMSAAQTDVITCTDGLSGGSGWKVSKMLKKDV 180

Query: 195 KNCSANVVRQISNALFLINNFNYK 215
           KNCS NVVRQISNALFLINNFNYK
Sbjct: 181 KNCSINVVRQISNALFLINNFNYK 203

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BLAST of Lsi05G013180.1 vs. TrEMBL
Match: M5X0K1_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011478mg PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 9.8e-26
Identity = 76/198 (38.38%), Postives = 120/198 (60.61%), Query Frame = 1


Query: 20  LKSLQTLFFFIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQT 79
           L  L  +   I +    T+   SSSS T +    +++ AC  T YP +C  +LS YAS+ 
Sbjct: 17  LTFLLPILLLIFMPNMHTTLATSSSSQTYKT---YVKTACNTTTYPLICYKSLSSYASKV 76

Query: 80  KRNQQELCRAAMASSLKAAQNATSIISKLSRRK-VSPYEAEVIGDCIDNLNDSVDELRRA 139
           K + Q+LC  A++ +LKAA+NA+S++SKL +   ++P E  V+ DCI+N+ DS+DEL+++
Sbjct: 77  KSDPQKLCTYALSVTLKAAKNASSVVSKLYKNTGLTPSEKGVVKDCIENIKDSIDELKQS 136

Query: 140 AAAIKSLSLK---LRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEVKNCSA 199
            +++ +L +    +++ L  IKTW SA +TD  TCTDG     G KV + +K  ++N   
Sbjct: 137 VSSMSNLGVSGSDVQLQLDDIKTWVSAVITDDATCTDGF---DGVKVSAAVKTAIRNSIV 196

Query: 200 NVVRQISNALFLINNFNY 214
           N  R  SNAL LI++  Y
Sbjct: 197 NAARLASNALSLIDSLIY 208

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BLAST of Lsi05G013180.1 vs. TrEMBL
Match: B9R9Q8_RICCO (21 kDa protein, putative OS=Ricinus communis GN=RCOM_1500020 PE=4 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 8.3e-25
Identity = 81/209 (38.76%), Postives = 126/209 (60.29%), Query Frame = 1


Query: 15  MESQILKS----LQTLFF--FIILTTFTTSAVASSSSSTVRPV-QPHIRKACKPTPYPRL 74
           ME++ + S    + TLF   F++  + T +++A +++ T +   + +++ AC  T YP++
Sbjct: 1   METKTITSPCLQVSTLFLITFLVFISNTQNSLAITTTKTSKTTYKNYLKTACNSTTYPKI 60

Query: 75  CETALSLYASQTKRNQQELCRAAMASSLKAAQNATSIISKLSRRK-VSPYEAEVIGDCID 134
           C   LS Y S  + N  +LC AA+  +LKAA N ++++  LS++K +S  E  VI DC  
Sbjct: 61  CYNTLSPYTSTIQTNDLKLCNAALTITLKAASNTSAMVKSLSKQKGLSKGEVAVIKDCQY 120

Query: 135 NLNDSVDELRRAAAAIKSL--SLKLRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRS 194
            + DSVDEL+++  A+K+L  S  +   + +IKTW SAA+TD  TCTDG     G KV S
Sbjct: 121 EIEDSVDELKQSLKALKNLKGSADMEFQIDNIKTWISAAITDENTCTDGF---EGMKVSS 180

Query: 195 KLKKEVKNCSANVVRQISNALFLINNFNY 214
           K+K ++K    NV R  SNAL LIN  +Y
Sbjct: 181 KVKSKIKKSIVNVNRLTSNALALINKLSY 206

[Close]

BLAST of Lsi05G013180.1 vs. TrEMBL
Match: F6HAT8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0022g00940 PE=4 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 2.4e-24
Identity = 83/210 (39.52%), Postives = 127/210 (60.48%), Query Frame = 1


Query: 15  MESQILKS------LQTLFFFIIL---TTFTTSAVASSSSSTVRPVQPHIRKACKPTPYP 74
           ME+ IL++      L +LFF ++L    T T+SA  S+ S T+   +  I+ +C  T YP
Sbjct: 1   MEACILRNPSAFHVLSSLFFTLLLFNSITLTSSANTSTISLTIS--KQFIKTSCNSTTYP 60

Query: 75  RLCETALSLYASQTKRNQQELCRAAMASSLKAAQNATSIISKLSRRK-VSPYEAEVIGDC 134
            +C  +LS YAS+ + +  +L   A+  SLKAA+NA+S I+ L  RK ++  E  ++ DC
Sbjct: 61  GVCYKSLSSYASKVQTSPLKLANVALTVSLKAARNASSTITLLLERKGMTRMETSIVKDC 120

Query: 135 IDNLNDSVDELRRAAAAIKSL--SLKLRILLSSIKTWASAALTDVITCTDGLSGGSGWKV 194
           ++N  D +DEL+++    KSL     +   +++IKTW SAALTD  TC+DG     G KV
Sbjct: 121 VENFGDCIDELQQSLQEFKSLGGGKNVAFQMANIKTWVSAALTDEYTCSDGF---EGQKV 180

Query: 195 RSKLKKEVKNCSANVVRQISNALFLINNFN 213
            S L++++KN  +NV +  SNAL LINN +
Sbjct: 181 SSSLQQQIKNYISNVAKITSNALALINNLS 205

[Close]

BLAST of Lsi05G013180.1 vs. TrEMBL
Match: A0A059DJ16_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A02882 PE=4 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 2.4e-24
Identity = 76/203 (37.44%), Postives = 128/203 (63.05%), Query Frame = 1


Query: 22  SLQTLFFFIIL----TTFTTSAVASSSSSTVRPVQPH--IRKACKPTPYPRLCETALSLY 81
           +L  L  F++L    +  T++A +SSSSS+ R  +    I+ +C  T YP+LC   LS Y
Sbjct: 5   NLHFLAIFLLLVANMSAITSAAASSSSSSSSRNGKDSNFIKTSCSTTTYPKLCNKYLSSY 64

Query: 82  ASQTKRNQQELCRAAMASSLKAAQNATSIISKLS-RRKVSPYEAEVIGDCIDNLNDSVDE 141
           AS  + N  +LC  A++ SL+AA+N +S+++ LS +R ++  EA ++ DCI+NL D++DE
Sbjct: 65  ASVVRSNPWKLCNCALSVSLQAARNTSSLVTTLSKKRSLTKAEASIVKDCIENLGDTIDE 124

Query: 142 LRRAAAAIKSLSLKLR----ILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEV 201
           L+++   + ++S   +      ++++KTW SAA+TD ITCTDG++     KV + +K+++
Sbjct: 125 LKQSIKTMANVSSAAKDDVAFQMANVKTWVSAAITDEITCTDGIAER---KVSAGVKRKI 184

Query: 202 KNCSANVVRQISNALFLINNFNY 214
           ++   NV R  SN L LIN+  Y
Sbjct: 185 QSYIENVARITSNGLSLINSLKY 204

[Close]

BLAST of Lsi05G013180.1 vs. TAIR10
Match: AT4G25250.1 (AT4G25250.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 96.7 bits (239), Expect = 1.9e-20
Identity = 62/192 (32.29%), Postives = 106/192 (55.21%), Query Frame = 1


Query: 25  TLFFFIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQ 84
           TL  FI  + F  +A  ++   T +    +++ AC  T YP +C   LS Y+S  K +  
Sbjct: 10  TLMVFINSSNFPKTA--ATPPGTYQNHTTYVKTACNSTTYPTMCYNCLSSYSSTIKSDPI 69

Query: 85  ELCRAAMASSLKAAQNATSIISKL--SRRKVSPYEAEVIGDCIDNLNDSVDELRRAAAAI 144
           +LC  ++  ++K+A+NAT ++S L    +    +E  ++ DC+D + D++DEL++A A +
Sbjct: 70  KLCTTSLNLNVKSAKNATLVVSNLLQKAKAAKSHEVSILKDCVDEMKDTIDELKQAVAEM 129

Query: 145 KSL---SLKLRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEVKNCSANVVR 204
           K +          L ++KTW S+ALTD  TCTDG   G   +V  + KK+VK   + + +
Sbjct: 130 KYVRGGGKTTEEHLKNVKTWVSSALTDEGTCTDGFEEG---RVNVETKKKVKKAISELSK 189

Query: 205 QISNALFLINNF 212
             SN L L+ ++
Sbjct: 190 TTSNTLALLTHY 196

[Close]

BLAST of Lsi05G013180.1 vs. TAIR10
Match: AT5G62350.1 (AT5G62350.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 96.7 bits (239), Expect = 1.9e-20
Identity = 65/187 (34.76%), Postives = 103/187 (55.08%), Query Frame = 1


Query: 31  ILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCRAA 90
           +L+   T+A A+S +   +     I+ +CK T YP LC  +LS+YA+  + + + L   A
Sbjct: 15  LLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAETA 74

Query: 91  MASSLKAAQNATSIISKLSRRK-VSPYEAEVIGDCIDNLNDSVDELRRAAAAIKSLSL-- 150
           +A +L  AQ+    +S+L+R K +   E E I DC++ +ND+VD L ++   +K      
Sbjct: 75  IAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRLTKSVQELKLCGSAK 134

Query: 151 ---KLRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEVKNCSANVVRQISNA 210
              +    +S+ +TW SAALTD  TC+DG SG     +  ++K  V+    NV  + SNA
Sbjct: 135 DQDQFAYHMSNAQTWTSAALTDENTCSDGFSGR---VMDGRIKNSVRARIMNVGHETSNA 194

Query: 211 LFLINNF 212
           L LIN F
Sbjct: 195 LSLINAF 198

[Close]

BLAST of Lsi05G013180.1 vs. TAIR10
Match: AT5G62360.1 (AT5G62360.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 94.0 bits (232), Expect = 1.2e-19
Identity = 60/185 (32.43%), Postives = 104/185 (56.22%), Query Frame = 1


Query: 28  FFIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQELC 87
           F II+         +++++T       ++ +C  T YPRLC ++LS +AS  + + + + 
Sbjct: 17  FLIIIAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMA 76

Query: 88  RAAMASSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLNDSVDELRRAAAAIKSLS- 147
            AA+  +L +A+  ++++ +LS  ++ P E   + DC++ L D+++ELR++   +  LS 
Sbjct: 77  HAALNITLASAKVTSAMMVRLSNSRLKPKEVSAMRDCVEELGDTLEELRKSIGEMCQLSG 136

Query: 148 LKLRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEVKNCSANVVRQISNALF 207
               + +S I+TW SAALTDV TCTDG  G     +  K+K  V+     +    SNAL 
Sbjct: 137 SNYEVYISDIQTWVSAALTDVNTCTDGFEGED---MDGKVKVLVRGRILVIAHLTSNALA 196

Query: 208 LINNF 212
           LIN+F
Sbjct: 197 LINHF 198

[Close]

BLAST of Lsi05G013180.1 vs. TAIR10
Match: AT5G51520.1 (AT5G51520.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 93.6 bits (231), Expect = 1.6e-19
Identity = 69/209 (33.01%), Postives = 112/209 (53.59%), Query Frame = 1


Query: 17  SQILKSLQTLFFFIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYA 76
           SQ+L SL  +F F   T    ++ ++SS S     +  ++ AC  T YP  C  +LS Y+
Sbjct: 2   SQVLYSLTIVFVFFASTNIQKTSGSASSYSQNH--KTFVKTACNSTTYPDKCYKSLSSYS 61

Query: 77  SQTKRNQQELCRAAMASSLKAAQNATSIISKL----------SRRKVSPYEAEVIGDCID 136
           S  K +  +LC  A+  ++K+A+ ATS++SKL           + K+ P EA ++ DC++
Sbjct: 62  SNIKSDPIKLCTTALNLNVKSAKEATSVVSKLLKMSQKSTAGRKGKMLP-EALILKDCLE 121

Query: 137 NLNDSVDELRRAAAAIKSL--SLKLRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRS 196
            + D++ EL++A   +K+L     +   +++++TW S+ALTD  TCTDG       KV  
Sbjct: 122 EMKDTIIELKQAITEMKNLQDGGSMAEHITNVRTWVSSALTDEGTCTDGF---EEVKVNK 181

Query: 197 KLKKEVKNCSANVVRQISNALFLINNFNY 214
           + KK+V      +    SN L LI N  Y
Sbjct: 182 ETKKKVNKVVEELATTTSNTLALITNLRY 204

[Close]

BLAST of Lsi05G013180.1 vs. TAIR10
Match: AT5G20740.1 (AT5G20740.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 86.3 bits (212), Expect = 2.5e-17
Identity = 63/192 (32.81%), Postives = 97/192 (50.52%), Query Frame = 1


Query: 29  FIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQELCR 88
           F++   F     AS+        +  +  +C+   YP LC   LS Y+  T  N+++L +
Sbjct: 8   FLVAIAFAVIFTASTVHGRHNGAEDIVHSSCEHASYPSLCVRTLSSYSGPTITNRRDLAQ 67

Query: 89  AAMASSLKAAQNATSIISKLSRRKVSPYEAE--VIGDCIDNLNDSVDELRRAAAAIKSLS 148
           AA+  SL  AQ+A   ++ + R  V   + E   + DC++ + DSVDEL R    +K L 
Sbjct: 68  AAIKISLSHAQSAAKKLAVV-RDSVGKKKQEKAALVDCVEMIGDSVDELSRTLGVLKHLR 127

Query: 149 L------KLRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEVKNCSANVVRQ 208
           +      + R  +S+ +TWASAALTD  TC DG  G        ++K EVK     V R 
Sbjct: 128 VSGGSAKEFRWQMSNAQTWASAALTDDDTCLDGFQGMDD----GEIKTEVKQWMTKVARV 187

Query: 209 ISNALFLINNFN 213
            SNAL+++N  +
Sbjct: 188 TSNALYMVNQLD 194

[Close]

BLAST of Lsi05G013180.1 vs. NCBI nr
Match: gi|449460810|ref|XP_004148137.1| (PREDICTED: 21 kDa protein-like [Cucumis sativus])

HSP 1 Score: 312.8 bits (800), Expect = 4.7e-82
Identity = 176/204 (86.27%), Postives = 181/204 (88.73%), Query Frame = 1


Query: 15  MESQILKSLQTL--FFFIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETAL 74
           MESQILKS  TL  FFFIILTTFT SAV SSSSSTVRPVQPHIRKACKPTPYPRLCETAL
Sbjct: 1   MESQILKSSLTLLIFFFIILTTFTPSAV-SSSSSTVRPVQPHIRKACKPTPYPRLCETAL 60

Query: 75  SLYASQTKRNQQELCRAAMASSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLNDSV 134
           SLYASQTKRNQQELCRAAM SSLKAAQNATSIISKLSRRK+S YEAEVIGDCIDNL DSV
Sbjct: 61  SLYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSV 120

Query: 135 DELRRAAAAIKSLSLK--LRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEV 194
           DELRRA+ AIKSLS    +   L+SIKTW SAA TDVITCTDGLSGGSGWKV   LKK+V
Sbjct: 121 DELRRASTAIKSLSRSKDVDFQLNSIKTWMSAAQTDVITCTDGLSGGSGWKVSKMLKKDV 180

Query: 195 KNCSANVVRQISNALFLINNFNYK 215
           KNCS NVVRQISNALFLINNFNYK
Sbjct: 181 KNCSINVVRQISNALFLINNFNYK 203

[Close]

BLAST of Lsi05G013180.1 vs. NCBI nr
Match: gi|659077238|ref|XP_008439099.1| (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 308.9 bits (790), Expect = 6.8e-81
Identity = 172/203 (84.73%), Postives = 181/203 (89.16%), Query Frame = 1


Query: 15  MESQILKSLQTLFFF-IILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALS 74
           MESQILKS  TLFFF IILTTFT SAV +SSSSTVRPV+PHIRKACKPTPYPRLCETALS
Sbjct: 1   MESQILKSSLTLFFFFIILTTFTPSAV-ASSSSTVRPVKPHIRKACKPTPYPRLCETALS 60

Query: 75  LYASQTKRNQQELCRAAMASSLKAAQNATSIISKLSRRKVSPYEAEVIGDCIDNLNDSVD 134
           LYASQTKRNQQELCRAAM SSLKAAQNATSIISKLSRRK+S YE EVIGDCID+L DSVD
Sbjct: 61  LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYETEVIGDCIDDLKDSVD 120

Query: 135 ELRRAAAAIKSLSLK--LRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEVK 194
           ELRRA+ AIKSLS    +   L+SIKTW SAALTD+ITCTDGLSGGSGWKV  K+KKEVK
Sbjct: 121 ELRRASTAIKSLSRSKDVDFQLNSIKTWTSAALTDIITCTDGLSGGSGWKVNKKVKKEVK 180

Query: 195 NCSANVVRQISNALFLINNFNYK 215
           NCS NVVRQISNAL LINNFNYK
Sbjct: 181 NCSINVVRQISNALSLINNFNYK 202

[Close]

BLAST of Lsi05G013180.1 vs. NCBI nr
Match: gi|470146900|ref|XP_004309057.1| (PREDICTED: 21 kDa protein-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 128.6 bits (322), Expect = 1.3e-26
Identity = 78/190 (41.05%), Postives = 119/190 (62.63%), Query Frame = 1


Query: 26  LFFFIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQTKRNQQE 85
           LF  ++   F  +  A+SSSS  R  + +++ AC  T YP++C  +LS YA+  K N Q+
Sbjct: 7   LFALLLFILFINTTTATSSSS--RTYKTYVKTACNSTTYPQICYKSLSSYATSIKSNPQK 66

Query: 86  LCRAAMASSLKAAQNATSIISKLSRRK-VSPYEAEVIGDCIDNLNDSVDELRRAAAAIKS 145
           LC  A++ +LKAA+ + S +SKL+++K ++  EA V+G CI+ + DSVDEL+++  A+K+
Sbjct: 67  LCSYALSVTLKAAKTSASTVSKLAKKKGITKAEAGVVGACIETIKDSVDELKQSVTAMKN 126

Query: 146 L-SLKLRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEVKNCSANVVRQISN 205
           L    ++  L +IKTW SA+LTD  TCTDG       KV + +K  ++     V R  SN
Sbjct: 127 LGGADMQYQLDNIKTWVSASLTDEDTCTDGF---DEQKVSTAVKNTIRKSILTVARLASN 186

Query: 206 ALFLINNFNY 214
           AL LI+N NY
Sbjct: 187 ALSLIDNLNY 191

[Close]

BLAST of Lsi05G013180.1 vs. NCBI nr
Match: gi|657945059|ref|XP_008377851.1| (PREDICTED: 21 kDa protein [Malus domestica])

HSP 1 Score: 128.3 bits (321), Expect = 1.7e-26
Identity = 77/199 (38.69%), Postives = 124/199 (62.31%), Query Frame = 1


Query: 19  ILKSLQTLFFFIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQ 78
           +L  L  +   II+T   T+   SSSS T +    +++ AC  T YP +C  +LS YAS+
Sbjct: 16  VLTFLLPIJLLIIMTNXQTTVATSSSSQTYKT---YVKTACNSTTYPLVCYQSLSSYASK 75

Query: 79  TKRNQQELCRAAMASSLKAAQNATSIISKLSRRK-VSPYEAEVIGDCIDNLNDSVDELRR 138
            K N ++LC  A++ +L+A++NA+S +SKLS+R  ++P E  +I DC++++ +S+DEL+ 
Sbjct: 76  VKSNPRKLCIYALSVTLQASKNASSXVSKLSKRAGLTPTEKGIINDCLESIKESIDELKD 135

Query: 139 AAAAIKSLSLK---LRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEVKNCS 198
           + +++K+L +K   ++  L  IKTW SA +TD +TCTDG     G KV + +K  + N  
Sbjct: 136 SLSSMKNLGVKGADMQAQLDDIKTWVSAVITDDVTCTDGF---DGLKVSTAVKTPINNSI 195

Query: 199 ANVVRQISNALFLINNFNY 214
             V R  SNAL LI++  Y
Sbjct: 196 VYVARLASNALSLIDSLXY 208

[Close]

BLAST of Lsi05G013180.1 vs. NCBI nr
Match: gi|645257369|ref|XP_008234383.1| (PREDICTED: 21 kDa protein-like [Prunus mume])

HSP 1 Score: 125.9 bits (315), Expect = 8.2e-26
Identity = 77/198 (38.89%), Postives = 119/198 (60.10%), Query Frame = 1


Query: 20  LKSLQTLFFFIILTTFTTSAVASSSSSTVRPVQPHIRKACKPTPYPRLCETALSLYASQT 79
           L  L  +   I +    T+   SSSS T +    +++ AC  T YP +C  +LS YAS+ 
Sbjct: 17  LTFLLPILLLIFMPNMHTTLATSSSSQTYKS---YVKTACNTTTYPLICYKSLSSYASKV 76

Query: 80  KRNQQELCRAAMASSLKAAQNATSIISKLSRRK-VSPYEAEVIGDCIDNLNDSVDELRRA 139
           K +  +LC  A++ +LKAA+NA+S++SKL +   ++P E  V+ DCIDN+ DS+DEL+++
Sbjct: 77  KSDPHKLCTYALSVTLKAAKNASSVVSKLYKNTGLTPSEKGVVKDCIDNIKDSIDELKQS 136

Query: 140 AAAIKSLSLK---LRILLSSIKTWASAALTDVITCTDGLSGGSGWKVRSKLKKEVKNCSA 199
            +++ +L +    +++ L  IKTW SA +TD  TCTDG     G KV + +K  +KN   
Sbjct: 137 VSSMSNLGVSGSDVQLQLDDIKTWVSAVITDDATCTDGF---DGVKVSTAVKTAIKNSIV 196

Query: 200 NVVRQISNALFLINNFNY 214
           N  R  SNAL LI++  Y
Sbjct: 197 NAARLASNALSLIDSLIY 208

The following BLAST results are available for this feature:

BLAST of Lsi05G013180.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
21KD_DAUCA	2.8e-18	33.84	21 kDa protein OS=Daucus carota PE=2 SV=1	[more]
PME2_ARATH	3.8e-07	25.48	Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2	[more]
PME61_ARATH	1.5e-06	28.57	Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana GN=P...	[more]

BLAST of Lsi05G013180.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L671_CUCSA	3.3e-82	86.27	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176250 PE=4 SV=1	[more]
M5X0K1_PRUPE	9.8e-26	38.38	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011478mg PE=4 SV=1	[more]
B9R9Q8_RICCO	8.3e-25	38.76	21 kDa protein, putative OS=Ricinus communis GN=RCOM_1500020 PE=4 SV=1	[more]
F6HAT8_VITVI	2.4e-24	39.52	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0022g00940 PE=4 SV=...	[more]
A0A059DJ16_EUCGR	2.4e-24	37.44	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A02882 PE=4 SV=1	[more]

BLAST of Lsi05G013180.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G25250.1	1.9e-20	32.29	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	1.9e-20	34.76	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62360.1	1.2e-19	32.43	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G51520.1	1.6e-19	33.01	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G20740.1	2.5e-17	32.81	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

BLAST of Lsi05G013180.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449460810\|ref\|XP_004148137.1\|	4.7e-82	86.27	PREDICTED: 21 kDa protein-like [Cucumis sativus]	[more]
gi\|659077238\|ref\|XP_008439099.1\|	6.8e-81	84.73	PREDICTED: 21 kDa protein-like [Cucumis melo]	[more]
gi\|470146900\|ref\|XP_004309057.1\|	1.3e-26	41.05	PREDICTED: 21 kDa protein-like [Fragaria vesca subsp. vesca]	[more]
gi\|657945059\|ref\|XP_008377851.1\|	1.7e-26	38.69	PREDICTED: 21 kDa protein [Malus domestica]	[more]
gi\|645257369\|ref\|XP_008234383.1\|	8.2e-26	38.89	PREDICTED: 21 kDa protein-like [Prunus mume]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR006501	Pectinesterase_inhib_dom

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi05G013180	Lsi05G013180	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi05G013180.1.three_prime_UTR.1	Lsi05G013180.1.three_prime_UTR.1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi05G013180.1.CDS.4	Lsi05G013180.1.CDS.4	CDS
Lsi05G013180.1.CDS.3	Lsi05G013180.1.CDS.3	CDS
Lsi05G013180.1.CDS.2	Lsi05G013180.1.CDS.2	CDS
Lsi05G013180.1.CDS.1	Lsi05G013180.1.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi05G013180.1	Lsi05G013180.1-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	G3DSA:1.20.140.40		coord: 54..211 score: 5.0
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 55..205 score: 6.9
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 50..207 score: 1.6
IPR006501	Pectinesterase inhibitor domain	TIGRFAMs	TIGR01614	TIGR01614	coord: 22..210 score: 3.6
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 53..211 score: 5.89
None	No IPR available	unknown	Coil	Coil	coord: 125..145 scor
None	No IPR available	PANTHER	PTHR31080	FAMILY NOT NAMED	coord: 15..210 score: 3.0
None	No IPR available	PANTHER	PTHR31080:SF15	SUBFAMILY NOT NAMED	coord: 15..210 score: 3.0