CmoCh11G007950 (gene) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh11G007950
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	23.2 kDa heat shock protein
Location	Cmo_Chr11 : 3938717 .. 3940523 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ACATAAGACATTGAAGATATCGATCGAAATCTCCGTCAAGAGAAGCGAAGAAAAAGACATCAGAATTACCGAAGAGGAAAATCAAATCGAACGCATCACCGATGGATTCGAAAAATTACCAGGATTTTTCGCCTAGATGTGATTGGCGTGAGGATCATGATTCTCGTGTTCTTACCGTACCCCTTAAAGGTTTGCTAATTCATCTCGCCTCCTCCTTCTTCGTTCTTTGCAATTCAGCAATTCGAAAACCGCACTCTAAAATTATTTTAAATAAAATTTTGAAAATTTATTTATATCCTTCAAATTTGACATTTAAATTTTTGTTGTAATCTCAATAAATTTTAAAATTTAAATTTAGTCATGAAATTATTATTATTTTTATACAAATTAGTAATAAAATTATTTTTTACATGTATTTTCAAATAAAAGATCTTATGAATGATTTTTTTCTCAACAATAAACTAACCATATAAATTAATATTAGGCCAATTTTAATGAAAATACAAAGGATAATTTTTATACTATAAATTTCAAAAACTAGAATAAGATTAAAATATAGGAGCTAAAATTAAAAAAAAAAAAAAAAAAAAACTATTTTTTAAAGACAAATCTTACTTTAAAATGAGTTATTATTTTATTAACATATTTAGACATATAAATATAAACACATAAATGAGAATAAAACAGAGAAAATAATAATAAATGAGAATAATAAATAAATAAATTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTTTTTTGAAGTTGCAGAGCTTTCTCCTTCTTCATCGAGTTTTGTTGTTCTACAGATTTTAGGAGCAGCCAACTGAAAGTCCAGGTGACATCCACCGGAAAGTTGAGGGTCTCCGGCGAACGGATGACCGAAGGCAACAAGTGGCTCCGATTCTACAAGGAGCTAGATGTCCCCACCGATACTGACACCGACAACATCTCTGCCAAGTTCAAGGATGGCGTCCTCTTCGTCAAACACCCCAAAAAACTATCTGCCGCTACTTTCCCCAAAGCCGATCCCCGACCACCGGCCGAGCCTCCGAAGCCAGCGGCCGGGAAACCCAAAGTCGATCCCCAACCACCGGCCGAGCCTCCGAAGCCGGGGGCCGGGAAACCCAAAGCTGATCCCCGACCACCGGCCGAGCCTCCGAAGCCAGCGGCCGCAAAATCCTCAATCGACCCACCAACTGATCCACAAAATGCCCCAAAGAGCCGAAATGAGGAACGCCAATCACAATCAAGTAGCAAAAGAGCCCCCACTTCCCCAAGGATACAAGGTATAGACAAAGAATTAGAGGATCAGCAGAATCTCACACCCGGCCCACAAACTGTCCCACAGAGACAAAATGAGGAGCCTCAAGAACAGGCACATGGGGAACGAAGCCCCCCTCCCCAAAAGGAAAAAACAGAGGAGGAGAAGGCAAAAGCACACACAAATCTGAAGGAAGCTTCGAAGGAGACAAGAGATGAGGAAAGGGGGAAAGAAGAAATTGGGTCGAAAATGGGAGAGGGCAAAGGGAAGGAGGAGGAGGGCAGCCATGGAAGGGAAGAGAAGGAAGGTGAAGTTGCAGAGGAGAGGAGGAGGAGGAGGAGGAGTGAGGGAACGGTGGAAGAGAGTTTCCTGTGGAGAAGAGAAGGGTACAAACAGGTTATTGATGGGGTGGTGAAGGAATTGAGGACAACTATGGTGACTTTGGTTTTGGGGATTGCTGTTCTTGTGATTCTGTATCTGAAAGTTACAAACAAAGGTCATGTGGATGAAGAATTGTGA

mRNA sequence

ACATAAGACATTGAAGATATCGATCGAAATCTCCGTCAAGAGAAGCGAAGAAAAAGACATCAGAATTACCGAAGAGGAAAATCAAATCGAACGCATCACCGATGGATTCGAAAAATTACCAGGATTTTTCGCCTAGATGTGATTGGCGTGAGGATCATGATTCTCGTGTTCTTACCGTACCCCTTAAAGATTTTAGGAGCAGCCAACTGAAAGTCCAGGTGACATCCACCGGAAAGTTGAGGGTCTCCGGCGAACGGATGACCGAAGGCAACAAGTGGCTCCGATTCTACAAGGAGCTAGATGTCCCCACCGATACTGACACCGACAACATCTCTGCCAAGTTCAAGGATGGCGTCCTCTTCGTCAAACACCCCAAAAAACTATCTGCCGCTACTTTCCCCAAAGCCGATCCCCGACCACCGGCCGAGCCTCCGAAGCCAGCGGCCGGGAAACCCAAAGTCGATCCCCAACCACCGGCCGAGCCTCCGAAGCCGGGGGCCGGGAAACCCAAAGCTGATCCCCGACCACCGGCCGAGCCTCCGAAGCCAGCGGCCGCAAAATCCTCAATCGACCCACCAACTGATCCACAAAATGCCCCAAAGAGCCGAAATGAGGAACGCCAATCACAATCAAGTAGCAAAAGAGCCCCCACTTCCCCAAGGATACAAGGTATAGACAAAGAATTAGAGGATCAGCAGAATCTCACACCCGGCCCACAAACTGTCCCACAGAGACAAAATGAGGAGCCTCAAGAACAGGCACATGGGGAACGAAGCCCCCCTCCCCAAAAGGAAAAAACAGAGGAGGAGAAGGCAAAAGCACACACAAATCTGAAGGAAGCTTCGAAGGAGACAAGAGATGAGGAAAGGGGGAAAGAAGAAATTGGGTCGAAAATGGGAGAGGGCAAAGGGAAGGAGGAGGAGGGCAGCCATGGAAGGGAAGAGAAGGAAGGTGAAGTTGCAGAGGAGAGGAGGAGGAGGAGGAGGAGTGAGGGAACGGTGGAAGAGAGTTTCCTGTGGAGAAGAGAAGGGTACAAACAGGTTATTGATGGGGTGGTGAAGGAATTGAGGACAACTATGGTGACTTTGGTTTTGGGGATTGCTGTTCTTGTGATTCTGTATCTGAAAGTTACAAACAAAGGTCATGTGGATGAAGAATTGTGA

Coding sequence (CDS)

ATGGATTCGAAAAATTACCAGGATTTTTCGCCTAGATGTGATTGGCGTGAGGATCATGATTCTCGTGTTCTTACCGTACCCCTTAAAGATTTTAGGAGCAGCCAACTGAAAGTCCAGGTGACATCCACCGGAAAGTTGAGGGTCTCCGGCGAACGGATGACCGAAGGCAACAAGTGGCTCCGATTCTACAAGGAGCTAGATGTCCCCACCGATACTGACACCGACAACATCTCTGCCAAGTTCAAGGATGGCGTCCTCTTCGTCAAACACCCCAAAAAACTATCTGCCGCTACTTTCCCCAAAGCCGATCCCCGACCACCGGCCGAGCCTCCGAAGCCAGCGGCCGGGAAACCCAAAGTCGATCCCCAACCACCGGCCGAGCCTCCGAAGCCGGGGGCCGGGAAACCCAAAGCTGATCCCCGACCACCGGCCGAGCCTCCGAAGCCAGCGGCCGCAAAATCCTCAATCGACCCACCAACTGATCCACAAAATGCCCCAAAGAGCCGAAATGAGGAACGCCAATCACAATCAAGTAGCAAAAGAGCCCCCACTTCCCCAAGGATACAAGGTATAGACAAAGAATTAGAGGATCAGCAGAATCTCACACCCGGCCCACAAACTGTCCCACAGAGACAAAATGAGGAGCCTCAAGAACAGGCACATGGGGAACGAAGCCCCCCTCCCCAAAAGGAAAAAACAGAGGAGGAGAAGGCAAAAGCACACACAAATCTGAAGGAAGCTTCGAAGGAGACAAGAGATGAGGAAAGGGGGAAAGAAGAAATTGGGTCGAAAATGGGAGAGGGCAAAGGGAAGGAGGAGGAGGGCAGCCATGGAAGGGAAGAGAAGGAAGGTGAAGTTGCAGAGGAGAGGAGGAGGAGGAGGAGGAGTGAGGGAACGGTGGAAGAGAGTTTCCTGTGGAGAAGAGAAGGGTACAAACAGGTTATTGATGGGGTGGTGAAGGAATTGAGGACAACTATGGTGACTTTGGTTTTGGGGATTGCTGTTCTTGTGATTCTGTATCTGAAAGTTACAAACAAAGGTCATGTGGATGAAGAATTGTGA

Homology

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BLAST of CmoCh11G007950 vs. Swiss-Prot
Match: HS232_ORYSJ (23.2 kDa heat shock protein OS=Oryza sativa subsp. japonica GN=HSP23.2 PE=2 SV=2)

HSP 1 Score: 55.5 bits (132), Expect = 1.4e-06
Identity = 33/96 (34.38%), Postives = 49/96 (51.04%), Query Frame = 1


Query: 12  RCDWREDHDSRVLTVPLKDFRSSQLKVQVTSTGKLRVSGERMTE--------GNKWL--- 71
           R DWRE  D+  + V +   R   L+V+V     LR+SGER  E        G+ W    
Sbjct: 77  RVDWRETGDAHEVVVDVPGMRKEDLRVEVEDNRVLRISGERRREETTEQKGGGDHWHREE 136

Query: 72  ----RFYKELDVPTDTDTDNISAKFKDGVLFVKHPK 93
               RF+++L +P + D D+I+A   +GVL V+  K
Sbjct: 137 RSYGRFWRQLRLPDNADLDSIAASLDNGVLTVRFRK 172

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BLAST of CmoCh11G007950 vs. Swiss-Prot
Match: HSP22_ARATH (22.0 kDa heat shock protein OS=Arabidopsis thaliana GN=HSP22.0 PE=2 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 3.1e-06
Identity = 27/92 (29.35%), Postives = 50/92 (54.35%), Query Frame = 1


Query: 12  RCDWREDHDSRVLTVPLKDFRSSQLKVQVTSTGKLRVSGERMTE----GNKWLR------ 71
           R DW+E  +   + + +   +  ++K++V   G LRVSGER  E    G++W R      
Sbjct: 72  RVDWKETAEGHEIMLDIPGLKKDEVKIEVEENGVLRVSGERKREEEKKGDQWHRVERSYG 131

Query: 72  -FYKELDVPTDTDTDNISAKFKDGVLFVKHPK 93
            F+++  +P + D +++ AK ++GVL +   K
Sbjct: 132 KFWRQFKLPDNVDMESVKAKLENGVLTINLTK 163

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BLAST of CmoCh11G007950 vs. Swiss-Prot
Match: HSP41_PEA (22.7 kDa class IV heat shock protein OS=Pisum sativum GN=HSP22.7 PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 4.1e-06
Identity = 28/86 (32.56%), Postives = 46/86 (53.49%), Query Frame = 1


Query: 12  RCDWREDHDSRVLTVPLKDFRSSQLKVQVTSTGKLRVSGERMTE----GNKWLR------ 71
           R DW+E  +  V+ V +   +   +K++V     LRVSGER  E    G+ W R      
Sbjct: 76  RVDWKETPEGHVIMVDVPGLKKDDIKIEVEENRVLRVSGERKKEEDKKGDHWHRVERSYG 135

Query: 72  -FYKELDVPTDTDTDNISAKFKDGVL 87
            F+++  +P + D D++ AK ++GVL
Sbjct: 136 KFWRQFKLPQNVDLDSVKAKMENGVL 161

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BLAST of CmoCh11G007950 vs. Swiss-Prot
Match: HSP41_SOYBN (22.0 kDa class IV heat shock protein OS=Glycine max GN=HSP22.0 PE=3 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 7.0e-06
Identity = 28/86 (32.56%), Postives = 47/86 (54.65%), Query Frame = 1


Query: 12  RCDWREDHDSRVLTVPLKDFRSSQLKVQVTSTGKLRVSGERMTE----GNKWLR------ 71
           R DW+E  +  V+ + +   +  ++KV+V     LRVSGER  E    G+ W R      
Sbjct: 68  RVDWKETPEGHVIMLDVPGLKREEIKVEVEENRVLRVSGERKKEEEKKGDHWHRVERSYG 127

Query: 72  -FYKELDVPTDTDTDNISAKFKDGVL 87
            F+++  +P + D D++ AK ++GVL
Sbjct: 128 KFWRQFRLPQNVDLDSVKAKLENGVL 153

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BLAST of CmoCh11G007950 vs. TrEMBL
Match: A0A0A0M2E7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G600110 PE=3 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 5.5e-50
Identity = 171/371 (46.09%), Postives = 211/371 (56.87%), Query Frame = 1


Query: 3   SKNYQDFSPRCDWREDHDSRVLTVPLKDFRSSQLKVQVTSTGKLRVSGERMTEGNKWLRF 62
           +K +++F PR DW +  D+RVL V L  FRS+QLKVQVTSTGKLRVSGER     KWLRF
Sbjct: 7   AKKFEEFEPRFDWVDHPDARVLVVHLSGFRSNQLKVQVTSTGKLRVSGERKLSSGKWLRF 66

Query: 63  YKELDVPTDTDTDNISAKFKDGVLFVKHPKKLSAATFPKADPRPPAEPPKPAAGKPKVDP 122
            KE+D+P D DTDNISAK + GVL+VK PKK SA +       PP + PKP     K   
Sbjct: 67  QKEIDIPADADTDNISAKLEQGVLYVKQPKKPSATS----SNIPPVQQPKP-----KAQS 126

Query: 123 QPPAEPPKPGAGKPKADPRPPA------EPPKPAAAKSSIDPPTDPQNAPKSRNEERQ-- 182
           QPP    KP A  P   P  P       EPPKPAA ++++ PPT   NAP+S+NE  Q  
Sbjct: 127 QPPPSATKPTADPPTVRPNTPKSQNERPEPPKPAATETTVAPPTVGPNAPESQNERAQIP 186

Query: 183 ------------------SQSSSKRAPTSPRIQGIDKELEDQQNLTPGPQTVPQRQNEEP 242
                             SQ+S K+ PT P+           +  T  P  +P+   E+ 
Sbjct: 187 YIKTNVKNYAPSQNDRPQSQASGKQIPTPPK----------PEKATGAPARIPKP--EKT 246

Query: 243 QEQAHGERSPPPQKEKTEEEKAKAHTNLKEASKETRDEERGKEEIGSKMGEGKGKEEEGS 302
                G+      K++  EEK KAHT L++A ++TR+E  GKEE      EG+ K EE  
Sbjct: 247 SSMGSGQPVEDLGKKEKTEEKGKAHTKLRDALEKTREE--GKEE------EGESKMEEKD 306

Query: 303 HGREEKEGEVAEERRRRRRSEGTVEESF-LWRREGYKQVIDGVVKELRTTMVTLVLGIAV 347
              +E+ GE    RRR+RRSE   EES  L RREGYKQVID VVKELRT MVTL LGIA 
Sbjct: 307 EKGKEEVGEEKRRRRRKRRSEEMGEESGRLRRREGYKQVIDVVVKELRTNMVTLALGIAA 348

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BLAST of CmoCh11G007950 vs. TrEMBL
Match: A0A0A0LWW1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G600100 PE=3 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.7e-43
Identity = 164/375 (43.73%), Postives = 212/375 (56.53%), Query Frame = 1


Query: 3   SKNYQDFSPRCDWREDHDSRVLTVPLKDFRSSQLKVQVTSTGKLRVSGERMTEGNKWLRF 62
           +KN+++F PR DW    DS VL V L DF+S+QL+VQVTSTGKLRVSG+RM     WLRF
Sbjct: 9   AKNFEEFEPRFDWVHHPDSHVLVVHLSDFKSNQLEVQVTSTGKLRVSGDRMLSNGTWLRF 68

Query: 63  YKELDVPTDTDTDNISAKFKDGVLFVKHPKKLSAAT-----FPKADPRPPAEPPKPAAGK 122
            KE+D+P D DTD ISAK + G+L+VK PKK SA +       ++ P+  ++ P PAA K
Sbjct: 69  EKEIDIPADADTDKISAKLEQGILYVKQPKKPSATSSNIPPVQQSKPKVQSQHP-PAATK 128

Query: 123 PKVDPQP--PAEPPKPGAGKPKAD--------PRPPAEPPKPAAAKSSID--------PP 182
           P  DP    P  P +   G P A            P    +      SID        P 
Sbjct: 129 PTADPPTVRPKAPNRRNEGLPTAFYPAIKTNLKNFPKSGDETGTPYPSIDAKKMHFHFPQ 188

Query: 183 TDPQNAPKSRNEERQSQSSSKRAPTSPRIQGIDKELEDQQNLTPGPQTVPQRQNEEPQEQ 242
           T   NAPKS+N+  QSQ+S K+ PT  ++Q        +    P         +  P E 
Sbjct: 189 TVKPNAPKSQNDRPQSQASGKQIPTPLKLQ--------EATGAPALGETSSTGSGHPVED 248

Query: 243 AHGERSPPPQKEKTEEEKAKAHTNLKEASKETRDEERGKEEIGSKMGEGKGKEEEGSHGR 302
                    +K+KTEE K K+HT L++A ++TR+E + +EE+GSKM E +         +
Sbjct: 249 L-------AKKDKTEE-KGKSHTKLRDAQEKTREEGK-EEEVGSKMAEKE---------K 308

Query: 303 EEKEGEVAEERRRRRRSEGTVEESF-LWRREGYKQVIDGVVKELRTTMVTLVLGIAVLVI 354
           EE E E    RR +RRSE   EES  L RREGYKQVID VVKELRT MVTL LGIA  V+
Sbjct: 309 EEVEEEKMRRRRMKRRSEEMGEESGRLRRREGYKQVIDVVVKELRTNMVTLALGIAAFVV 356

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BLAST of CmoCh11G007950 vs. TrEMBL
Match: A0A0A0LZR6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G600120 PE=3 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.4e-24
Identity = 126/321 (39.25%), Postives = 167/321 (52.02%), Query Frame = 1


Query: 53  MTEGNKWLRFYKELDVPTDTDTDNISAKFKDGVLFVKHPKKLSAATF---PKADPRPPAE 112
           +T G KWLRF KE+D+P D DTD IS+K + G+L+VK PKK SA +    P  + R   E
Sbjct: 39  LTNG-KWLRFQKEIDIPDDADTDKISSKLEQGILYVKQPKKPSATSSNIPPSQNERD--E 98

Query: 113 PPKPAAGKPKVDPQPPAEPPKPGAGKPKADPRP-----------------PAEPPKPAAA 172
           P KPAA +P V       PP  G+  P++   P                  A+ PKPAA 
Sbjct: 99  PLKPAATEPTV------APPTVGSNAPESQSEPRSWIIIPAPDFRSFRNERADTPKPAAT 158

Query: 173 KSSIDPPTDPQNAPKSRNEERQSQSSSKRAPTSPRIQGIDKELEDQQNLTPGPQTVPQRQ 232
           KS++ PPT   NAPKS+N+  +SQ+S K+ PT P+           +  T  P  +P  +
Sbjct: 159 KSTVYPPTVRPNAPKSQNDRPKSQASGKQIPTPPK----------PEEATGAPARIP--K 218

Query: 233 NEEPQEQAHGERSPPPQKEKTEEEKAKAHTNLKEASKETRDEERGKEEIGSKMGEGKGKE 292
           + E      G+      K++  E+K K HT L++A ++TR   R        +  GK K 
Sbjct: 219 SGETSSMGCGQPVEDLAKKEKTEDKGKIHTKLRDALEKTRRRRR--------LRRGKKKL 278

Query: 293 EEGSHGREEKEGEVAEERRRRRRSEGTVEESFLWRREGYKQVIDGVVKELRTTMVTLVLG 352
           E+    R E+ GE               E   L RREGYKQV+DGVVKELRT MVTL LG
Sbjct: 279 EK----RSEEMGE---------------ESGRLRRREGYKQVVDGVVKELRTNMVTLALG 307

Query: 353 IAVLVILYLKVTNKGHVDEEL 354
               VILYL ++ KGH +EEL
Sbjct: 339 ----VILYLNLSKKGHTEEEL 307

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BLAST of CmoCh11G007950 vs. TrEMBL
Match: A0A087G8W1_ARAAL (Uncharacterized protein OS=Arabis alpina GN=AALP_AA8G231400 PE=3 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.8e-17
Identity = 49/106 (46.23%), Postives = 65/106 (61.32%), Query Frame = 1


Query: 6   YQDFSPRCDWREDHDSRVLTVPLKDFRSSQLKVQVTSTGKLRVSGERMTEGNKWLRFYKE 65
           YQ+F P   W  + D+ VL   L  F+  QLKV VT+T KLR+SGER T GNKW+RF++E
Sbjct: 10  YQNFEPATRWTSEPDAEVLVADLPGFKKEQLKVAVTATRKLRLSGERPTLGNKWIRFHQE 69

Query: 66  LDVPTDTDTDNISAKFKDGVLFVKHPKKLSAATFPKADPRPPAEPP 112
           + VP   D D +SA FKD  L+++HPK       P+     P +PP
Sbjct: 70  IPVPMTADIDTVSAMFKDNKLYIRHPK--IKTEIPQTKTPSPVKPP 113

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BLAST of CmoCh11G007950 vs. TrEMBL
Match: F4K6X6_ARATH (Heat shock family protein OS=Arabidopsis thaliana GN=At5g20970 PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.5e-15
Identity = 57/136 (41.91%), Postives = 75/136 (55.15%), Query Frame = 1


Query: 6   YQDFSPRCDWREDHDSRVLTVPLKDFRSSQLKVQVTSTGKLRVSGERMTEGNKWLRFYKE 65
           YQ+F P   W  + D+ VL   L  F+  QLKV VT+T KLR++GER T GNKW+RF++E
Sbjct: 10  YQEFEPATRWTSEPDAEVLVADLPGFKKEQLKVSVTATRKLRLTGERPTGGNKWIRFHQE 69

Query: 66  LDVPTDTDTDNISAKFKDGVLFVKHPKKLSAATFPKADPRPPAEPPKPAAGKP------K 125
           + VP   D D++SA FKD  L+++HPK  +          P  +PP P   KP      K
Sbjct: 70  IPVPLTVDIDSVSAMFKDNKLYIRHPKLKTEI--------PQTKPPTPVIMKPHDQHERK 129

Query: 126 VDPQPPAEPPKPGAGK 136
               P A   KP  GK
Sbjct: 130 QGQGPKAMVEKPSGGK 137

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BLAST of CmoCh11G007950 vs. TAIR10
Match: AT5G20970.1 (AT5G20970.1 HSP20-like chaperones superfamily protein)

HSP 1 Score: 92.0 bits (227), Expect = 7.7e-19
Identity = 57/136 (41.91%), Postives = 75/136 (55.15%), Query Frame = 1


Query: 6   YQDFSPRCDWREDHDSRVLTVPLKDFRSSQLKVQVTSTGKLRVSGERMTEGNKWLRFYKE 65
           YQ+F P   W  + D+ VL   L  F+  QLKV VT+T KLR++GER T GNKW+RF++E
Sbjct: 10  YQEFEPATRWTSEPDAEVLVADLPGFKKEQLKVSVTATRKLRLTGERPTGGNKWIRFHQE 69

Query: 66  LDVPTDTDTDNISAKFKDGVLFVKHPKKLSAATFPKADPRPPAEPPKPAAGKP------K 125
           + VP   D D++SA FKD  L+++HPK  +          P  +PP P   KP      K
Sbjct: 70  IPVPLTVDIDSVSAMFKDNKLYIRHPKLKTEI--------PQTKPPTPVIMKPHDQHERK 129

Query: 126 VDPQPPAEPPKPGAGK 136
               P A   KP  GK
Sbjct: 130 QGQGPKAMVEKPSGGK 137

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BLAST of CmoCh11G007950 vs. TAIR10
Match: AT2G27140.1 (AT2G27140.1 HSP20-like chaperones superfamily protein)

HSP 1 Score: 66.2 bits (160), Expect = 4.5e-11
Identity = 58/179 (32.40%), Postives = 85/179 (47.49%), Query Frame = 1


Query: 6   YQDFSPRCDWREDHDSRVLTVPLKDFRSSQLKVQVTSTGKLRVSGERMTEGNKWLRFYKE 65
           Y +F P  +W+ +     LT+ L  FR  QLKVQVT+T KLRV G+R    NKW+RF KE
Sbjct: 14  YDEFEPLSNWKTEQGFEALTIYLPGFRKEQLKVQVTTTRKLRVMGDRPAGANKWIRFRKE 73

Query: 66  LDVPTDTDTDNISAKFKDGVLFVKHPKKLSAATFPKADPRPPAEPPKPAAGKPKVDPQPP 125
             +P + D D++SAKF+   L V+ P+             P  + P P     K  P P 
Sbjct: 74  FPIPPNIDVDSVSAKFEGANLVVRLPR-----------TEPMGKQPSPIGTATKPPPVPK 133

Query: 126 AEP--PKPGAGKPKADPRPPAEPPKPAAAKSSIDPPTDPQNAPKSRNEERQSQSSSKRA 183
             P  P P A K K  P     P +    ++ ++   +   +PK   E  ++ +   R+
Sbjct: 134 ENPNLPSPSA-KEKVQP-----PKETRENETELEKQAEKIQSPKPARENEEALNQDYRS 175

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BLAST of CmoCh11G007950 vs. TAIR10
Match: AT1G54400.1 (AT1G54400.1 HSP20-like chaperones superfamily protein)

HSP 1 Score: 60.8 bits (146), Expect = 1.9e-09
Identity = 32/93 (34.41%), Postives = 47/93 (50.54%), Query Frame = 1


Query: 6   YQDFSPRCDWREDHDSRVLTVPLKD-FRSSQLKVQVTSTGKLRVSGERMTEGNKWLRFYK 65
           Y +  P C WR   D  +L + L    +   LK+Q+ ++G L ++G    +  K +RF K
Sbjct: 13  YDEIEPFCRWRRTEDIDILELHLPSGLKKEHLKIQINNSGVLTITGGCPVDQTKTIRFMK 72

Query: 66  ELDVPTDTDTDNISAKFKDGVLFVKHPKKLSAA 98
           E  V  D   + I AKF  GVL+V  PK +  A
Sbjct: 73  ETKVAKDCKRNEIRAKFSKGVLYVTMPKTIPIA 105

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BLAST of CmoCh11G007950 vs. TAIR10
Match: AT4G10250.1 (AT4G10250.1 HSP20-like chaperones superfamily protein)

HSP 1 Score: 54.3 bits (129), Expect = 1.8e-07
Identity = 27/92 (29.35%), Postives = 50/92 (54.35%), Query Frame = 1


Query: 12  RCDWREDHDSRVLTVPLKDFRSSQLKVQVTSTGKLRVSGERMTE----GNKWLR------ 71
           R DW+E  +   + + +   +  ++K++V   G LRVSGER  E    G++W R      
Sbjct: 72  RVDWKETAEGHEIMLDIPGLKKDEVKIEVEENGVLRVSGERKREEEKKGDQWHRVERSYG 131

Query: 72  -FYKELDVPTDTDTDNISAKFKDGVLFVKHPK 93
            F+++  +P + D +++ AK ++GVL +   K
Sbjct: 132 KFWRQFKLPDNVDMESVKAKLENGVLTINLTK 163

[Close]

BLAST of CmoCh11G007950 vs. NCBI nr
Match: gi|659100301|ref|XP_008451025.1| (PREDICTED: pollen-specific leucine-rich repeat extensin-like protein 1 isoform X1 [Cucumis melo])

HSP 1 Score: 210.7 bits (535), Expect = 4.2e-51
Identity = 172/383 (44.91%), Postives = 214/383 (55.87%), Query Frame = 1


Query: 3   SKNYQDFSPRCDWREDHDSRVLTVPLKDFRSSQLKVQVTSTGKLRVSGERMTEGNKWLRF 62
           +KN+++F PR DW    DS VL V L  FRS+QLKVQVTSTGKLRVSGER     KWLRF
Sbjct: 7   AKNFEEFEPRFDWVHHPDSHVLVVHLSGFRSNQLKVQVTSTGKLRVSGERKLGNGKWLRF 66

Query: 63  YKELDVPTDTDTDNISAKFKDGVLFVKHPKKLSAATFPKADPRPPAEPPKPAAGKPKVDP 122
            KE+D+P D DTD ISAK ++G+L+VK PKKL   +       PP + PKP     K   
Sbjct: 67  EKEIDIPADADTDKISAKLEEGILYVKQPKKLPTTS----SNIPPVQQPKP-----KAQS 126

Query: 123 QPPAEPPKPGAGKPKADPRPP------AEPPKPAAAKSSIDPPTDPQNAPKSRNEERQSQ 182
           QPP    KP A  P   P  P      AEPPKPAA + ++ PPT    AP+S+NE     
Sbjct: 127 QPPPAATKPTADPPTVRPSVPKSRNERAEPPKPAATEPTVAPPTVGPKAPESQNERADVP 186

Query: 183 SSSKRAPTSPRIQGIDKELEDQQNLTPGP---QTVPQRQNEEPQEQAHGERSPPP----- 242
           S+             D +  + +N  P P      P+ QN+ PQ QA G++ P P     
Sbjct: 187 ST-------------DAKKTNVKNYAPRPTVGPNAPKSQNDRPQSQASGKQIPTPPKPEE 246

Query: 243 -----------------QKEKTEEEKAKAHTNLKEASKETRDEERGKEEIGSKMGEGKGK 302
                            +K+KT EEK KAHT L++A ++TR+E  GKE            
Sbjct: 247 ATGAPSMGSGQPVEDLAKKDKT-EEKGKAHTKLQDALEKTREE--GKE------------ 306

Query: 303 EEEGSHGREEKEGEVAEERRRRRRSEGTVEE-----SFLWRREGYKQVIDGVVKELRTTM 350
           EE GS   EE++ EV EE+RRRRR +   EE       L RREGYKQVIDGVVKE+RT M
Sbjct: 307 EEGGSKKAEEQKEEVGEEKRRRRRMKRRNEEMGEESGRLRRREGYKQVIDGVVKEIRTNM 352

[Close]

BLAST of CmoCh11G007950 vs. NCBI nr
Match: gi|778665351|ref|XP_004144143.2| (PREDICTED: inactive protein RESTRICTED TEV MOVEMENT 2-like [Cucumis sativus])

HSP 1 Score: 206.5 bits (524), Expect = 7.9e-50
Identity = 171/371 (46.09%), Postives = 211/371 (56.87%), Query Frame = 1


Query: 3   SKNYQDFSPRCDWREDHDSRVLTVPLKDFRSSQLKVQVTSTGKLRVSGERMTEGNKWLRF 62
           +K +++F PR DW +  D+RVL V L  FRS+QLKVQVTSTGKLRVSGER     KWLRF
Sbjct: 7   AKKFEEFEPRFDWVDHPDARVLVVHLSGFRSNQLKVQVTSTGKLRVSGERKLSSGKWLRF 66

Query: 63  YKELDVPTDTDTDNISAKFKDGVLFVKHPKKLSAATFPKADPRPPAEPPKPAAGKPKVDP 122
            KE+D+P D DTDNISAK + GVL+VK PKK SA +       PP + PKP     K   
Sbjct: 67  QKEIDIPADADTDNISAKLEQGVLYVKQPKKPSATS----SNIPPVQQPKP-----KAQS 126

Query: 123 QPPAEPPKPGAGKPKADPRPPA------EPPKPAAAKSSIDPPTDPQNAPKSRNEERQ-- 182
           QPP    KP A  P   P  P       EPPKPAA ++++ PPT   NAP+S+NE  Q  
Sbjct: 127 QPPPSATKPTADPPTVRPNTPKSQNERPEPPKPAATETTVAPPTVGPNAPESQNERAQIP 186

Query: 183 ------------------SQSSSKRAPTSPRIQGIDKELEDQQNLTPGPQTVPQRQNEEP 242
                             SQ+S K+ PT P+           +  T  P  +P+   E+ 
Sbjct: 187 YIKTNVKNYAPSQNDRPQSQASGKQIPTPPK----------PEKATGAPARIPKP--EKT 246

Query: 243 QEQAHGERSPPPQKEKTEEEKAKAHTNLKEASKETRDEERGKEEIGSKMGEGKGKEEEGS 302
                G+      K++  EEK KAHT L++A ++TR+E  GKEE      EG+ K EE  
Sbjct: 247 SSMGSGQPVEDLGKKEKTEEKGKAHTKLRDALEKTREE--GKEE------EGESKMEEKD 306

Query: 303 HGREEKEGEVAEERRRRRRSEGTVEESF-LWRREGYKQVIDGVVKELRTTMVTLVLGIAV 347
              +E+ GE    RRR+RRSE   EES  L RREGYKQVID VVKELRT MVTL LGIA 
Sbjct: 307 EKGKEEVGEEKRRRRRKRRSEEMGEESGRLRRREGYKQVIDVVVKELRTNMVTLALGIAA 348

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BLAST of CmoCh11G007950 vs. NCBI nr
Match: gi|659100303|ref|XP_008451026.1| (PREDICTED: inactive protein RESTRICTED TEV MOVEMENT 2-like isoform X2 [Cucumis melo])

HSP 1 Score: 206.5 bits (524), Expect = 7.9e-50
Identity = 170/380 (44.74%), Postives = 211/380 (55.53%), Query Frame = 1


Query: 3   SKNYQDFSPRCDWREDHDSRVLTVPLKDFRSSQLKVQVTSTGKLRVSGERMTEGNKWLRF 62
           +KN+++F PR DW    DS VL V L  FRS+QLKVQVTSTGKLRVSGER     KWLRF
Sbjct: 7   AKNFEEFEPRFDWVHHPDSHVLVVHLSGFRSNQLKVQVTSTGKLRVSGERKLGNGKWLRF 66

Query: 63  YKELDVPTDTDTDNISAKFKDGVLFVKHPKKLSAATFPKADPRPPAEPPKPAAGKPKVDP 122
            KE+D+P D DTD ISAK ++G+L+VK PKKL   +       PP + PKP     K   
Sbjct: 67  EKEIDIPADADTDKISAKLEEGILYVKQPKKLPTTS----SNIPPVQQPKP-----KAQS 126

Query: 123 QPPAEPPKPGAGKPKADPRPP------AEPPKPAAAKSSIDPPTDPQNAPKSRNEERQSQ 182
           QPP    KP A  P   P  P      AEPPKPAA + ++ PPT    AP+S+NE     
Sbjct: 127 QPPPAATKPTADPPTVRPSVPKSRNERAEPPKPAATEPTVAPPTVGPKAPESQNERADVP 186

Query: 183 SSSKRAPTSPRIQGIDKELEDQQNLTPGPQTVPQRQNEEPQEQAHGERSPPP-------- 242
           S+             D +  + +N  P        QN+ PQ QA G++ P P        
Sbjct: 187 ST-------------DAKKTNVKNYAPS-------QNDRPQSQASGKQIPTPPKPEEATG 246

Query: 243 --------------QKEKTEEEKAKAHTNLKEASKETRDEERGKEEIGSKMGEGKGKEEE 302
                         +K+KT EEK KAHT L++A ++TR+E  GKE            EE 
Sbjct: 247 APSMGSGQPVEDLAKKDKT-EEKGKAHTKLQDALEKTREE--GKE------------EEG 306

Query: 303 GSHGREEKEGEVAEERRRRRRSEGTVEE-----SFLWRREGYKQVIDGVVKELRTTMVTL 350
           GS   EE++ EV EE+RRRRR +   EE       L RREGYKQVIDGVVKE+RT MVTL
Sbjct: 307 GSKKAEEQKEEVGEEKRRRRRMKRRNEEMGEESGRLRRREGYKQVIDGVVKEIRTNMVTL 342

[Close]

BLAST of CmoCh11G007950 vs. NCBI nr
Match: gi|700211274|gb|KGN66370.1| (hypothetical protein Csa_1G600100 [Cucumis sativus])

HSP 1 Score: 184.9 bits (468), Expect = 2.5e-43
Identity = 164/375 (43.73%), Postives = 212/375 (56.53%), Query Frame = 1


Query: 3   SKNYQDFSPRCDWREDHDSRVLTVPLKDFRSSQLKVQVTSTGKLRVSGERMTEGNKWLRF 62
           +KN+++F PR DW    DS VL V L DF+S+QL+VQVTSTGKLRVSG+RM     WLRF
Sbjct: 9   AKNFEEFEPRFDWVHHPDSHVLVVHLSDFKSNQLEVQVTSTGKLRVSGDRMLSNGTWLRF 68

Query: 63  YKELDVPTDTDTDNISAKFKDGVLFVKHPKKLSAAT-----FPKADPRPPAEPPKPAAGK 122
            KE+D+P D DTD ISAK + G+L+VK PKK SA +       ++ P+  ++ P PAA K
Sbjct: 69  EKEIDIPADADTDKISAKLEQGILYVKQPKKPSATSSNIPPVQQSKPKVQSQHP-PAATK 128

Query: 123 PKVDPQP--PAEPPKPGAGKPKAD--------PRPPAEPPKPAAAKSSID--------PP 182
           P  DP    P  P +   G P A            P    +      SID        P 
Sbjct: 129 PTADPPTVRPKAPNRRNEGLPTAFYPAIKTNLKNFPKSGDETGTPYPSIDAKKMHFHFPQ 188

Query: 183 TDPQNAPKSRNEERQSQSSSKRAPTSPRIQGIDKELEDQQNLTPGPQTVPQRQNEEPQEQ 242
           T   NAPKS+N+  QSQ+S K+ PT  ++Q        +    P         +  P E 
Sbjct: 189 TVKPNAPKSQNDRPQSQASGKQIPTPLKLQ--------EATGAPALGETSSTGSGHPVED 248

Query: 243 AHGERSPPPQKEKTEEEKAKAHTNLKEASKETRDEERGKEEIGSKMGEGKGKEEEGSHGR 302
                    +K+KTEE K K+HT L++A ++TR+E + +EE+GSKM E +         +
Sbjct: 249 L-------AKKDKTEE-KGKSHTKLRDAQEKTREEGK-EEEVGSKMAEKE---------K 308

Query: 303 EEKEGEVAEERRRRRRSEGTVEESF-LWRREGYKQVIDGVVKELRTTMVTLVLGIAVLVI 354
           EE E E    RR +RRSE   EES  L RREGYKQVID VVKELRT MVTL LGIA  V+
Sbjct: 309 EEVEEEKMRRRRMKRRSEEMGEESGRLRRREGYKQVIDVVVKELRTNMVTLALGIAAFVV 356

[Close]

BLAST of CmoCh11G007950 vs. NCBI nr
Match: gi|659100306|ref|XP_008451028.1| (PREDICTED: pollen-specific leucine-rich repeat extensin-like protein 1 [Cucumis melo])

HSP 1 Score: 157.5 bits (397), Expect = 4.2e-35
Identity = 138/316 (43.67%), Postives = 169/316 (53.48%), Query Frame = 1


Query: 3   SKNYQDFSPRCDWREDHDSRVLTVPLKDFRSSQLKVQVTSTGKLRVSGERMTEGNKWLRF 62
           +KN+Q+F PR DW    DS VL V L  FRS+QLKVQVTSTGKLRVSGER     KWLRF
Sbjct: 7   AKNFQEFEPRYDWVHHPDSHVLVVRLSGFRSNQLKVQVTSTGKLRVSGERKLGNGKWLRF 66

Query: 63  YKELDVPTDTDTDNISAKFKDGVLFVKHPKKLSAATF---PKADPRP-PAEPPKPAAGKP 122
            KE+D+P D DTD ISAK ++G+L+VK PKKLSA +    P   P+P P   P PAA KP
Sbjct: 67  EKEIDIPADADTDKISAKLEEGILYVKQPKKLSATSSNIPPVQQPKPKPQSQPPPAATKP 126

Query: 123 KVDPQPPAEPPKPGAGKPKADPRPPAEPPKPAAAKSSIDPPTDPQNAPKSRNEERQSQSS 182
             DP P   P  P +   +      +EPPKPAA + ++ PPT    AP+S+NE       
Sbjct: 127 TADP-PTVRPSAPKSQNER------SEPPKPAATEPTVAPPTVGPKAPESQNERAD---- 186

Query: 183 SKRAPTSPRIQGIDKELEDQQNLTPGP---QTVPQRQNEEPQEQAHGERSPPPQK----- 242
                    I  ID    +  N  P P      P+ QN+ PQ QA G++ P P K     
Sbjct: 187 ---------IPSIDARKTNYINYAPRPTVGPNAPKSQNDRPQSQASGKQIPTPPKPEEAT 246

Query: 243 ---------------------EKTEEEKAKAHTNLKEASKETRDEERGKEEIGSKMGEGK 286
                                EKT EEK KAHT L++A ++TR+E  GKEE G     G 
Sbjct: 247 GAPAETSSMGSGQPVEDLAKNEKT-EEKGKAHTKLQDAVEKTREE--GKEEEGGSKNGGG 297

The following BLAST results are available for this feature:

BLAST of CmoCh11G007950 vs. Swiss-Prot


Match Name	E-value	Identity	Description
HS232_ORYSJ	1.4e-06	34.38	23.2 kDa heat shock protein OS=Oryza sativa subsp. japonica GN=HSP23.2 PE=2 SV=2	[more]
HSP22_ARATH	3.1e-06	29.35	22.0 kDa heat shock protein OS=Arabidopsis thaliana GN=HSP22.0 PE=2 SV=1	[more]
HSP41_PEA	4.1e-06	32.56	22.7 kDa class IV heat shock protein OS=Pisum sativum GN=HSP22.7 PE=2 SV=1	[more]
HSP41_SOYBN	7.0e-06	32.56	22.0 kDa class IV heat shock protein OS=Glycine max GN=HSP22.0 PE=3 SV=1	[more]

BLAST of CmoCh11G007950 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0M2E7_CUCSA	5.5e-50	46.09	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G600110 PE=3 SV=1	[more]
A0A0A0LWW1_CUCSA	1.7e-43	43.73	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G600100 PE=3 SV=1	[more]
A0A0A0LZR6_CUCSA	1.4e-24	39.25	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G600120 PE=3 SV=1	[more]
A0A087G8W1_ARAAL	2.8e-17	46.23	Uncharacterized protein OS=Arabis alpina GN=AALP_AA8G231400 PE=3 SV=1	[more]
F4K6X6_ARATH	1.5e-15	41.91	Heat shock family protein OS=Arabidopsis thaliana GN=At5g20970 PE=3 SV=1	[more]

BLAST of CmoCh11G007950 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G20970.1	7.7e-19	41.91	HSP20-like chaperones superfamily protein	[more]
AT2G27140.1	4.5e-11	32.40	HSP20-like chaperones superfamily protein	[more]
AT1G54400.1	1.9e-09	34.41	HSP20-like chaperones superfamily protein	[more]
AT4G10250.1	1.8e-07	29.35	HSP20-like chaperones superfamily protein	[more]

BLAST of CmoCh11G007950 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659100301\|ref\|XP_008451025.1\|	4.2e-51	44.91	PREDICTED: pollen-specific leucine-rich repeat extensin-like protein 1 isoform X...	[more]
gi\|778665351\|ref\|XP_004144143.2\|	7.9e-50	46.09	PREDICTED: inactive protein RESTRICTED TEV MOVEMENT 2-like [Cucumis sativus]	[more]
gi\|659100303\|ref\|XP_008451026.1\|	7.9e-50	44.74	PREDICTED: inactive protein RESTRICTED TEV MOVEMENT 2-like isoform X2 [Cucumis m...	[more]
gi\|700211274\|gb\|KGN66370.1\|	2.5e-43	43.73	hypothetical protein Csa_1G600100 [Cucumis sativus]	[more]
gi\|659100306\|ref\|XP_008451028.1\|	4.2e-35	43.67	PREDICTED: pollen-specific leucine-rich repeat extensin-like protein 1 [Cucumis ...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002068	A-crystallin/Hsp20_dom
IPR008978	HSP20-like_chaperone

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh11G007950.1	CmoCh11G007950.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002068	Alpha crystallin/Hsp20 domain	PFAM	PF00011	HSP20	coord: 14..96 score: 3.
IPR002068	Alpha crystallin/Hsp20 domain	PROFILE	PS01031	HSP20	coord: 14..92 score: 11
IPR008978	HSP20-like chaperone	GENE3D	G3DSA:2.60.40.790		coord: 5..95 score: 1.4
IPR008978	HSP20-like chaperone	unknown	SSF49764	HSP20-like chaperones	coord: 5..93 score: 6.28
None	No IPR available	unknown	Coil	Coil	coord: 230..250 scor
None	No IPR available	PANTHER	PTHR11527:SF156	HEAT SHOCK FAMILY PROTEIN	coord: 6..115 score: 4.0

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmoCh11G007950	CmaCh10G010000	Cucurbita maxima (Rimu)	cmacmoB074
CmoCh11G007950	CmaCh11G007900	Cucurbita maxima (Rimu)	cmacmoB112
CmoCh11G007950	Cla011096	Watermelon (97103) v1	cmowmB088
CmoCh11G007950	Csa1G600080	Cucumber (Chinese Long) v2	cmocuB075
CmoCh11G007950	MELO3C015587	Melon (DHL92) v3.5.1	cmomeB097
CmoCh11G007950	ClCG03G014410	Watermelon (Charleston Gray)	cmowcgB101
CmoCh11G007950	CSPI01G29330	Wild cucumber (PI 183967)	cmocpiB078
CmoCh11G007950	Lsi06G004090	Bottle gourd (USVL1VR-Ls)	cmolsiB118
CmoCh11G007950	MELO3C015587.2	Melon (DHL92) v3.6.1	cmomedB104
CmoCh11G007950	CsaV3_1G041730	Cucumber (Chinese Long) v3	cmocucB0090
CmoCh11G007950	Cla97C03G064610	Watermelon (97103) v2	cmowmbB100
CmoCh11G007950	Bhi02G001439	Wax gourd	cmowgoB0159
CmoCh11G007950	Carg06128	Silver-seed gourd	carcmoB0028
CmoCh11G007950	Carg18028	Silver-seed gourd	carcmoB0241

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
CmoCh11G007950	CmoCh10G010540	Cucurbita moschata (Rifu)	cmocmoB044

The following block(s) are covering this gene:


Gene	Organism	Block
CmoCh11G007950	Cucumber (Gy14) v2	cgybcmoB027
CmoCh11G007950	Cucumber (Gy14) v1	cgycmoB0396
CmoCh11G007950	Cucurbita pepo (Zucchini)	cmocpeB104
CmoCh11G007950	Cucurbita pepo (Zucchini)	cmocpeB129