CmaCh17G008190 (gene) Cucurbita maxima (Rimu)

NameCmaCh17G008190
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionTo encode a PR protein, Belongs to the plant thionin family with the following members:, putative
LocationCma_Chr17 : 6436238 .. 6436825 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGTCAATTGTTCTAATTTGCTTCATTCTGAGCTTAGTGGCAGGAAGGTCCACAGCGTCCTTTAGGAAGTGCTATGCCAAGTGCTTCGTAGTGTGTGCTATCACACCAGGCATCACCCTTGGCACCTGCGGTGCAACGTGCTTGGCGAAGTGCCTGTTCATTGCATCTGCCCCAATGGATTTCAACCATATGGACACACATTACTTCTGCAAACTTGGGTGTGCCACTTCCATGTGCACCAAATTCAGCACCAAGAACGACCCTTGTGAGCAAAATTTCCTGTACCCATTTCTTCGTTTCTTATTTTTCAAACATGTTTTCATTTTGTGTTATGAACTTGGCAGCTGAGAAGAAAGTGGAAAGGTGTGTGGACTCCTGCGCTGGAACATGCATCAAAGCTTAGAAACATGAAGAAGGGAGGAGGAGCTGGGATTGGCCTTAACACCATCAATAAATAAACATATTTTGGTGTATGCATGGTTTCAAGTCTCTGCCCGTGTTATGTGAACCATATGCCTTAAATGTTTAAAAACTTATTAGACACAAAATTTGATCTCAAAGTTTGAGGGGCCAAGAGCTCTAAAC

mRNA sequence

ATGAAGTCAATTGTTCTAATTTGCTTCATTCTGAGCTTAGTGGCAGGAAGGTCCACAGCGTCCTTTAGGAAGTGCTATGCCAAGTGCTTCGTAGTGTGTGCTATCACACCAGGCATCACCCTTGGCACCTGCGGTGCAACGTGCTTGGCGAAGTGCCTGTTCATTGCATCTGCCCCAATGGATTTCAACCATATGGACACACATTACTTCTGCAAACTTGGGTGTGCCACTTCCATGTGCACCAAATTCAGCACCAAGAACGACCCTTCTGAGAAGAAAGTGGAAAGGTGTGTGGACTCCTGCGCTGGAACATGCATCAAAGCTTAGAAACATGAAGAAGGGAGGAGGAGCTGGGATTGGCCTTAACACCATCAATAAATAAACATATTTTGGTGTATGCATGGTTTCAAGTCTCTGCCCGTGTTATGTGAACCATATGCCTTAAATGTTTAAAAACTTATTAGACACAAAATTTGATCTCAAAGTTTGAGGGGCCAAGAGCTCTAAAC

Coding sequence (CDS)

ATGAAGTCAATTGTTCTAATTTGCTTCATTCTGAGCTTAGTGGCAGGAAGGTCCACAGCGTCCTTTAGGAAGTGCTATGCCAAGTGCTTCGTAGTGTGTGCTATCACACCAGGCATCACCCTTGGCACCTGCGGTGCAACGTGCTTGGCGAAGTGCCTGTTCATTGCATCTGCCCCAATGGATTTCAACCATATGGACACACATTACTTCTGCAAACTTGGGTGTGCCACTTCCATGTGCACCAAATTCAGCACCAAGAACGACCCTTCTGAGAAGAAAGTGGAAAGGTGTGTGGACTCCTGCGCTGGAACATGCATCAAAGCTTAG

Protein sequence

MKSIVLICFILSLVAGRSTASFRKCYAKCFVVCAITPGITLGTCGATCLAKCLFIASAPMDFNHMDTHYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTCIKA
BLAST of CmaCh17G008190 vs. Swiss-Prot
Match: THNL2_ARATH (Thionin-like protein 2 OS=Arabidopsis thaliana GN=At1g12663 PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 1.6e-09
Identity = 39/109 (35.78%), Postives = 59/109 (54.13%), Query Frame = 1

Query: 5   VLICFILSLVAGRSTAS---FRKCYAKCFVVC-AITPGITLGTCGATCLAKCLFIASAP- 64
           V+I  ++  +  ++ A    F++CY  C V C A +       C  TC  +CL   S P 
Sbjct: 5   VMIVMVIGNLLAQTAAQKIPFKECYPACLVECKAGSKFPKYLKCPFTCTKECLQQPSPPS 64

Query: 65  MDFNHMD-THYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTCIK 108
           +  N++D + YFCKLGCAT  C   S+  +P+ ++V  CVDSC+  C K
Sbjct: 65  VSSNNIDESDYFCKLGCATYHCVSLSSIQNPNVERVSACVDSCSNKCTK 113

BLAST of CmaCh17G008190 vs. TrEMBL
Match: A0A0A0KC18_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G446640 PE=4 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 6.6e-39
Identity = 78/92 (84.78%), Postives = 84/92 (91.30%), Query Frame = 1

Query: 1  MKSIVLICFILSLVAGRSTASFRKCYAKCFVVCAITPGITLGTCGATCLAKCLFIASAPM 60
          MKS+VLICFILSLVAGRSTASF KCYAKCF+VCAITPGI +GTCGA CLA CLFIAS+PM
Sbjct: 1  MKSVVLICFILSLVAGRSTASFGKCYAKCFIVCAITPGIPVGTCGAKCLADCLFIASSPM 60

Query: 61 DFNHMDTHYFCKLGCATSMCTKFSTKNDPSEK 93
          D N+MDTHYFCKLGCATS CTKFSTK DP +K
Sbjct: 61 DLNYMDTHYFCKLGCATSRCTKFSTKKDPGKK 92

BLAST of CmaCh17G008190 vs. TrEMBL
Match: A0A059AI82_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_J03010 PE=4 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 6.7e-23
Identity = 52/105 (49.52%), Postives = 72/105 (68.57%), Query Frame = 1

Query: 1   MKSIVLICFILSLVAGRSTASFRKCYAKCFVVCAITPGITLGTCGATCLAKCLFIASAPM 60
           +KS+ ++C +L L  G+S A+F+ CY  CF++CAITPG TL +C   CL  C+   S  +
Sbjct: 4   VKSVFMVCLVLGLFLGQSGANFQDCYPACFILCAITPGRTLFSCSVECLKDCIIPPSDSL 63

Query: 61  DFNHMDTHYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTC 106
                DT YFCKLGCA+S+CT  STK++P EK+V  CV+SC+ TC
Sbjct: 64  SLR--DTMYFCKLGCASSLCTNLSTKDNPREKRVAGCVNSCSKTC 106

BLAST of CmaCh17G008190 vs. TrEMBL
Match: F6HX25_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g04840 PE=4 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 8.7e-23
Identity = 50/105 (47.62%), Postives = 73/105 (69.52%), Query Frame = 1

Query: 1   MKSIVLICFILSLVAGRSTASFRKCYAKCFVVCAITPGITLGTCGATCLAKCLFIASAPM 60
           ++++V++  ++ ++ G+STASF+ CY KCF+ C ITP  T  +C   CL  C+ I S P+
Sbjct: 6   IRALVMVGLVMGMLVGQSTASFKDCYTKCFIFCIITPNNTAFSCSLECLKDCV-IPSHPL 65

Query: 61  DFNHMDTHYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTC 106
           D    DT YFCKLGCA+S+CT  STK +P+ +KVE CV+SC+  C
Sbjct: 66  DIETADTTYFCKLGCASSLCTNLSTKKNPAGEKVESCVNSCSNKC 109

BLAST of CmaCh17G008190 vs. TrEMBL
Match: A0A061GI20_THECC (To encode a PR protein, Belongs to the plant thionin family with the following members:, putative OS=Theobroma cacao GN=TCM_028643 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 2.5e-22
Identity = 52/105 (49.52%), Postives = 75/105 (71.43%), Query Frame = 1

Query: 3   SIVLICFILSLVAGRSTASFRKCYAKCFVVCAITPGITLGTCGATCLAKCLFIASAPMDF 62
           S++++C +L L+ G+STASF+KCYA CF++C   P   + +C   CL +C+ I S+ ++ 
Sbjct: 47  SVMMVCLVLGLLVGQSTASFQKCYAGCFLLCVFIPPNPVFSCAIKCLNECI-IPSSTLE- 106

Query: 63  NHMDTHYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTCIK 108
           +  DT YFCKLGCATS+CT  S+K +P E+KV  CVDSC+ TC K
Sbjct: 107 SGKDTQYFCKLGCATSLCTDLSSKENPGEQKVGSCVDSCSETCAK 149

BLAST of CmaCh17G008190 vs. TrEMBL
Match: A0A067E0U5_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g033731mg PE=4 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 3.3e-22
Identity = 50/105 (47.62%), Postives = 74/105 (70.48%), Query Frame = 1

Query: 3   SIVLICFILSLVAGRSTASFRKCYAKCFVVCAITPGITLGTCGATCLAKCLFIASAPMDF 62
           S++++  +  L+ G+S A+F++CY  CF++C ITPG TLG+C A CL  C+F        
Sbjct: 8   SLLMVYLMTGLLVGQSQAAFKECYETCFIICYITPGTTLGSCAAKCLKDCIFPPKL-HTL 67

Query: 63  NHMDTHYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTCIK 108
           +  DT YFCKLGCATS+CT  S+K++P+ +KVE CV+SC+ +C K
Sbjct: 68  SLQDTEYFCKLGCATSLCTNLSSKDNPAGEKVEGCVNSCSKSCSK 111

BLAST of CmaCh17G008190 vs. TAIR10
Match: AT1G12663.1 (AT1G12663.1 Predicted to encode a PR (pathogenesis-related) protein. Belongs to the plant thionin (PR-13) family with the following members: At1g66100, At5g36910, At1g72260, At2g15010, At1g12663, At1g12660.)

HSP 1 Score: 63.5 bits (153), Expect = 8.9e-11
Identity = 39/109 (35.78%), Postives = 59/109 (54.13%), Query Frame = 1

Query: 5   VLICFILSLVAGRSTAS---FRKCYAKCFVVC-AITPGITLGTCGATCLAKCLFIASAP- 64
           V+I  ++  +  ++ A    F++CY  C V C A +       C  TC  +CL   S P 
Sbjct: 5   VMIVMVIGNLLAQTAAQKIPFKECYPACLVECKAGSKFPKYLKCPFTCTKECLQQPSPPS 64

Query: 65  MDFNHMD-THYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTCIK 108
           +  N++D + YFCKLGCAT  C   S+  +P+ ++V  CVDSC+  C K
Sbjct: 65  VSSNNIDESDYFCKLGCATYHCVSLSSIQNPNVERVSACVDSCSNKCTK 113

BLAST of CmaCh17G008190 vs. TAIR10
Match: AT1G12672.2 (AT1G12672.2 unknown protein)

HSP 1 Score: 53.5 bits (127), Expect = 9.2e-08
Identity = 27/63 (42.86%), Postives = 36/63 (57.14%), Query Frame = 1

Query: 47  TCLAKCLFIASAPMD----FNHMDTHYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCA 106
           +C+  CL   S+ MD     NH D  Y+CKLGC+T  C   S+  +P+  KV  CVDSC+
Sbjct: 40  SCIKTCLEPPSSQMDSTNEINHND--YYCKLGCSTHHCASLSSIQNPNVDKVVDCVDSCS 99

BLAST of CmaCh17G008190 vs. NCBI nr
Match: gi|700190113|gb|KGN45346.1| (hypothetical protein Csa_7G446640 [Cucumis sativus])

HSP 1 Score: 167.9 bits (424), Expect = 9.5e-39
Identity = 78/92 (84.78%), Postives = 84/92 (91.30%), Query Frame = 1

Query: 1  MKSIVLICFILSLVAGRSTASFRKCYAKCFVVCAITPGITLGTCGATCLAKCLFIASAPM 60
          MKS+VLICFILSLVAGRSTASF KCYAKCF+VCAITPGI +GTCGA CLA CLFIAS+PM
Sbjct: 1  MKSVVLICFILSLVAGRSTASFGKCYAKCFIVCAITPGIPVGTCGAKCLADCLFIASSPM 60

Query: 61 DFNHMDTHYFCKLGCATSMCTKFSTKNDPSEK 93
          D N+MDTHYFCKLGCATS CTKFSTK DP +K
Sbjct: 61 DLNYMDTHYFCKLGCATSRCTKFSTKKDPGKK 92

BLAST of CmaCh17G008190 vs. NCBI nr
Match: gi|449470525|ref|XP_004152967.1| (PREDICTED: thionin-like protein 2 [Cucumis sativus])

HSP 1 Score: 132.5 bits (332), Expect = 4.4e-28
Identity = 66/111 (59.46%), Postives = 80/111 (72.07%), Query Frame = 1

Query: 1   MKSIVLICFILSLVAGRSTASFR--KCYAKCFVVCAITPGI-TLGTCGATCLAKCLFIAS 60
           M+S+VLICFILSLVAG+STA     KCY  C  VCA+   + T+G C   C A C+ IAS
Sbjct: 1   MRSVVLICFILSLVAGKSTAVIEVSKCYLNCMAVCAVPSSLLTVGLCALKCTADCIAIAS 60

Query: 61  APMDFNHMDTHYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTCIKA 109
           +PM+ N +DT YFCKLGCATS CTK STK D +EKKVE CV++C   C+KA
Sbjct: 61  SPMNLNRVDTRYFCKLGCATSCCTKSSTKKDFAEKKVESCVNTCDQICMKA 111

BLAST of CmaCh17G008190 vs. NCBI nr
Match: gi|659131068|ref|XP_008465492.1| (PREDICTED: thionin-like protein 2 [Cucumis melo])

HSP 1 Score: 121.7 bits (304), Expect = 7.8e-25
Identity = 65/111 (58.56%), Postives = 76/111 (68.47%), Query Frame = 1

Query: 1   MKSIVLICFILSLVAGRSTASF--RKCYAKCFVVCAITPGI-TLGTCGATCLAKCLFIAS 60
           M+SIVLI FILSLVAGRST      K + KC   CAI P I  +  C   C+A C+ +AS
Sbjct: 1   MRSIVLIHFILSLVAGRSTPVLDTAKWFVKCMAECAILPIIPVVSICAVKCMADCIPVAS 60

Query: 61  APMDFNHMDTHYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTCIKA 109
           +PMD N MDTHYFCKLGC T  CTK STK D +EKKVE  V++C  TC+KA
Sbjct: 61  SPMDLNRMDTHYFCKLGCTTYRCTKLSTKKDLAEKKVESGVNTCDQTCMKA 111

BLAST of CmaCh17G008190 vs. NCBI nr
Match: gi|702484105|ref|XP_010033893.1| (PREDICTED: thionin-like protein 2 [Eucalyptus grandis])

HSP 1 Score: 114.8 bits (286), Expect = 9.6e-23
Identity = 52/105 (49.52%), Postives = 72/105 (68.57%), Query Frame = 1

Query: 1   MKSIVLICFILSLVAGRSTASFRKCYAKCFVVCAITPGITLGTCGATCLAKCLFIASAPM 60
           +KS+ ++C +L L  G+S A+F+ CY  CF++CAITPG TL +C   CL  C+   S  +
Sbjct: 4   VKSVFMVCLVLGLFLGQSGANFQDCYPACFILCAITPGRTLFSCSVECLKDCIIPPSDSL 63

Query: 61  DFNHMDTHYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTC 106
                DT YFCKLGCA+S+CT  STK++P EK+V  CV+SC+ TC
Sbjct: 64  SLR--DTMYFCKLGCASSLCTNLSTKDNPREKRVAGCVNSCSKTC 106

BLAST of CmaCh17G008190 vs. NCBI nr
Match: gi|225442677|ref|XP_002284798.1| (PREDICTED: thionin-like protein 2 [Vitis vinifera])

HSP 1 Score: 114.4 bits (285), Expect = 1.2e-22
Identity = 50/105 (47.62%), Postives = 73/105 (69.52%), Query Frame = 1

Query: 1   MKSIVLICFILSLVAGRSTASFRKCYAKCFVVCAITPGITLGTCGATCLAKCLFIASAPM 60
           ++++V++  ++ ++ G+STASF+ CY KCF+ C ITP  T  +C   CL  C+ I S P+
Sbjct: 6   IRALVMVGLVMGMLVGQSTASFKDCYTKCFIFCIITPNNTAFSCSLECLKDCV-IPSHPL 65

Query: 61  DFNHMDTHYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTC 106
           D    DT YFCKLGCA+S+CT  STK +P+ +KVE CV+SC+  C
Sbjct: 66  DIETADTTYFCKLGCASSLCTNLSTKKNPAGEKVESCVNSCSNKC 109

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
THNL2_ARATH1.6e-0935.78Thionin-like protein 2 OS=Arabidopsis thaliana GN=At1g12663 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KC18_CUCSA6.6e-3984.78Uncharacterized protein OS=Cucumis sativus GN=Csa_7G446640 PE=4 SV=1[more]
A0A059AI82_EUCGR6.7e-2349.52Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_J03010 PE=4 SV=1[more]
F6HX25_VITVI8.7e-2347.62Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g04840 PE=4 SV=... [more]
A0A061GI20_THECC2.5e-2249.52To encode a PR protein, Belongs to the plant thionin family with the following m... [more]
A0A067E0U5_CITSI3.3e-2247.62Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g033731mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G12663.18.9e-1135.78 Predicted to encode a PR (pathogenesis-related) protein. Belongs to... [more]
AT1G12672.29.2e-0842.86 unknown protein[more]
Match NameE-valueIdentityDescription
gi|700190113|gb|KGN45346.1|9.5e-3984.78hypothetical protein Csa_7G446640 [Cucumis sativus][more]
gi|449470525|ref|XP_004152967.1|4.4e-2859.46PREDICTED: thionin-like protein 2 [Cucumis sativus][more]
gi|659131068|ref|XP_008465492.1|7.8e-2558.56PREDICTED: thionin-like protein 2 [Cucumis melo][more]
gi|702484105|ref|XP_010033893.1|9.6e-2349.52PREDICTED: thionin-like protein 2 [Eucalyptus grandis][more]
gi|225442677|ref|XP_002284798.1|1.2e-2247.62PREDICTED: thionin-like protein 2 [Vitis vinifera][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
cellular_component GO:0016020 membrane
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh17G008190.1CmaCh17G008190.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR36312FAMILY NOT NAMEDcoord: 15..106
score: 2.3
NoneNo IPR availablePANTHERPTHR36312:SF1PLANT THIONIN FAMILY PROTEIN-RELATEDcoord: 15..106
score: 2.3

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh17G008190CmaCh08G006800Cucurbita maxima (Rimu)cmacmaB383