CmaCh04G005220 (gene) Cucurbita maxima (Rimu)

NameCmaCh04G005220
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionrRNA N-glycosidase
LocationCma_Chr04 : 2641610 .. 2642461 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAGACGAGACAAGTCCTCTTGTGTATGGTCGTGGCATTCTCCCTCGCCACCCACATCGGCACCACCCTCGGGTATGGAGCTTTCGGAGGACGCGCACATCTTGTGGGTCGAGATGGGTTGTGTTTACAGACAGCGCCGCTTTCCATATATTACACTCCAACTAAATTAACAGACTGCGATGAATCGGACTCGGACCAATTATGGGCGATCGTTGATGATGGCACAATTCGGGGAGATAATGATAGAAATTGTTTGGTTCCTCATAGTCGGGGTATTAAACCCATTGGAACCAACGTCGTACTAGTAGACTGTACGAAAGAGATAAGAAGCGATATGAAATGGACCAAAACGAACGACGGTACCATACGTCATGACGAGTCGGGGCTCGTCTTGACGGGGAAACAAGGCCAGTATGTGTCGTTGGAAGTCGATGAAGGTGCGCCGTCACAGGGCTGGGAAGCCACGGAAAGGCCCTACCCAAAGGTGGCCAACATCAAGTGGCATAACAACTTGTGTTTACAATTTGGAGATGGTGAATTTTCGTTGGGGCTGAGTGCGTGTAGTAGCAAATATAATAAGAGCCAGCGATGGGTGCTGTATGGGGATGGCACCATTCGAGGCAATGGCGATAGCAATGTCTGCTTGACTTCGCTTTTAAGCTCTTCGGTGGTTGTGTCTAAGTGCGCAGACATGCCACAACAGCGTTGGGGGCTTGCAGCGGACAACACCATCGGCCATCCCAACACCAACTTGGTCATGGATGCGCGTACGTTTGTGCGTCTCTCGCCACTCATCCTGGTTGCCAAGCGCACTGGGACTTATAGCCAACAATGGATCGTTTATTGA

mRNA sequence

ATGGAGAAGACGAGACAAGTCCTCTTGTGTATGGTCGTGGCATTCTCCCTCGCCACCCACATCGGCACCACCCTCGGGTATGGAGCTTTCGGAGGACGCGCACATCTTGTGGGTCGAGATGGGTTGTGTTTACAGACAGCGCCGCTTTCCATATATTACACTCCAACTAAATTAACAGACTGCGATGAATCGGACTCGGACCAATTATGGGCGATCGTTGATGATGGCACAATTCGGGGAGATAATGATAGAAATTGTTTGGTTCCTCATAGTCGGGGTATTAAACCCATTGGAACCAACGTCGTACTAGTAGACTGTACGAAAGAGATAAGAAGCGATATGAAATGGACCAAAACGAACGACGGTACCATACGTCATGACGAGTCGGGGCTCGTCTTGACGGGGAAACAAGGCCAGTATGTGTCGTTGGAAGTCGATGAAGGTGCGCCGTCACAGGGCTGGGAAGCCACGGAAAGGCCCTACCCAAAGGTGGCCAACATCAAGTGGCATAACAACTTGTGTTTACAATTTGGAGATGGTGAATTTTCGTTGGGGCTGAGTGCGTGTAGTAGCAAATATAATAAGAGCCAGCGATGGGTGCTGTATGGGGATGGCACCATTCGAGGCAATGGCGATAGCAATGTCTGCTTGACTTCGCTTTTAAGCTCTTCGGTGGTTGTGTCTAAGTGCGCAGACATGCCACAACAGCGTTGGGGGCTTGCAGCGGACAACACCATCGGCCATCCCAACACCAACTTGGTCATGGATGCGCGTACGTTTGTGCGTCTCTCGCCACTCATCCTGGTTGCCAAGCGCACTGGGACTTATAGCCAACAATGGATCGTTTATTGA

Coding sequence (CDS)

ATGGAGAAGACGAGACAAGTCCTCTTGTGTATGGTCGTGGCATTCTCCCTCGCCACCCACATCGGCACCACCCTCGGGTATGGAGCTTTCGGAGGACGCGCACATCTTGTGGGTCGAGATGGGTTGTGTTTACAGACAGCGCCGCTTTCCATATATTACACTCCAACTAAATTAACAGACTGCGATGAATCGGACTCGGACCAATTATGGGCGATCGTTGATGATGGCACAATTCGGGGAGATAATGATAGAAATTGTTTGGTTCCTCATAGTCGGGGTATTAAACCCATTGGAACCAACGTCGTACTAGTAGACTGTACGAAAGAGATAAGAAGCGATATGAAATGGACCAAAACGAACGACGGTACCATACGTCATGACGAGTCGGGGCTCGTCTTGACGGGGAAACAAGGCCAGTATGTGTCGTTGGAAGTCGATGAAGGTGCGCCGTCACAGGGCTGGGAAGCCACGGAAAGGCCCTACCCAAAGGTGGCCAACATCAAGTGGCATAACAACTTGTGTTTACAATTTGGAGATGGTGAATTTTCGTTGGGGCTGAGTGCGTGTAGTAGCAAATATAATAAGAGCCAGCGATGGGTGCTGTATGGGGATGGCACCATTCGAGGCAATGGCGATAGCAATGTCTGCTTGACTTCGCTTTTAAGCTCTTCGGTGGTTGTGTCTAAGTGCGCAGACATGCCACAACAGCGTTGGGGGCTTGCAGCGGACAACACCATCGGCCATCCCAACACCAACTTGGTCATGGATGCGCGTACGTTTGTGCGTCTCTCGCCACTCATCCTGGTTGCCAAGCGCACTGGGACTTATAGCCAACAATGGATCGTTTATTGA

Protein sequence

MEKTRQVLLCMVVAFSLATHIGTTLGYGAFGGRAHLVGRDGLCLQTAPLSIYYTPTKLTDCDESDSDQLWAIVDDGTIRGDNDRNCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTNDGTIRHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKVANIKWHNNLCLQFGDGEFSLGLSACSSKYNKSQRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRWGLAADNTIGHPNTNLVMDARTFVRLSPLILVAKRTGTYSQQWIVY
BLAST of CmaCh04G005220 vs. Swiss-Prot
Match: RIP1_SAMNI (Ribosome-inactivating protein SNAI' OS=Sambucus nigra PE=1 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 3.6e-29
Identity = 92/254 (36.22%), Postives = 124/254 (48.82%), Query Frame = 1

Query: 38  GRDGLCLQTAPLSIYY-TPTKLTDCDESDSDQLWAIVDDGTIRGDNDRNCLVPHSRGIKP 97
           GRDG C +         TP +L+ C E  S+Q W    DGTI+      CL   S     
Sbjct: 323 GRDGFCAEVKNGDEKDGTPVQLSSCGEQ-SNQQWTFSTDGTIQSLG--KCLTTSS----- 382

Query: 98  IGTNVVLVDCTKEIRSDMKWTKTNDGTIRHDESGLVLTGK---QGQYVSLEVDEGAPSQG 157
              +V++ +C        KW  + DGTI +  SGLVLT     +G  VSLE +  A  QG
Sbjct: 383 ---SVMIYNCKVVPPESTKWVVSIDGTITNPRSGLVLTAPKAAEGTLVSLEKNVHAARQG 442

Query: 158 WEATERPYPKVANIKWHNNLCLQFGDGEFSLGLSACSSKYNKS--QRWVLYGDGTIRGNG 217
           W       P V  I  +  +CL+   G   + L  CS K      Q+W LYGDGTIR N 
Sbjct: 443 W-IVGNVEPLVTFIVGYEQMCLETNPGNNDVSLGDCSVKSASKVDQKWALYGDGTIRVNN 502

Query: 218 DSNVCLTSLLSSS---VVVSKCADMPQQRWGLAADNTIGHPNTNLVMDA-RTFVRLSPLI 277
           D ++C+TS   SS   +++ KC     QRW    D TI +P++ LVM   +  V L  +I
Sbjct: 503 DRSLCVTSEGKSSNEPIIILKCLGWANQRWVFNTDGTISNPDSKLVMHVDQNDVPLRKII 562

Query: 278 LVAKRTGTYSQQWI 282
           L +  +GT +QQWI
Sbjct: 563 L-SHPSGTSNQQWI 563

BLAST of CmaCh04G005220 vs. Swiss-Prot
Match: ML3_VISAL (Beta-galactoside-specific lectin 3 OS=Viscum album PE=1 SV=2)

HSP 1 Score: 115.2 bits (287), Expect = 1.2e-24
Identity = 79/255 (30.98%), Postives = 119/255 (46.67%), Query Frame = 1

Query: 36  LVGRDGLCLQTAPLSIYY-TPTKLTDC-DESDSDQLWAIVDDGTIRGDNDRNCLVPHSRG 95
           +VGRDGLC+       +   P +L+ C   +D +QLW I  DGTIR +    CL  +   
Sbjct: 320 IVGRDGLCVDVRDGKFHNGNPIQLSPCKSNTDPNQLWTIRRDGTIRSNG--RCLTTYGY- 379

Query: 96  IKPIGTNVVLVDCTKEIRSDMKWTKTNDGTIRHDESGLVL---TGKQGQYVSLEVDEGAP 155
               G  V++ DC   +R    W    +GTI +  S LVL   +G  G  ++++    + 
Sbjct: 380 --TAGVYVMIFDCNTAVREATLWQIWGNGTIINPRSNLVLGAASGSSGTTLTVQTQVYSL 439

Query: 156 SQGWEATERPYPKVANIKWHNNLCLQFGDGEFSLGLSACSSKYNKSQRWVLYGDGTIRGN 215
            QGW A     P+   I    +LC++      S+ +  C S   ++Q W LYGDG+IR  
Sbjct: 440 GQGWLAGNDTAPREVTIYGFRDLCMEANGA--SVWVETCGSS-TENQNWALYGDGSIRPK 499

Query: 216 GDSNVCLT----SLLSSSVVVSKCADMPQQRWGLAADNTIGHPNTNLVMDARTFVRLSPL 275
            + + CLT    S+ +   +VS  A    QRW    + TI + N  LVMD          
Sbjct: 500 QNQDQCLTCQGDSVATVINIVSCSAGSSGQRWVFTNEGTILNLNNGLVMDVAQSNPSLRR 559

Query: 276 ILVAKRTGTYSQQWI 282
           I++   TG  +Q W+
Sbjct: 560 IIIYPATGNPNQMWL 566

BLAST of CmaCh04G005220 vs. Swiss-Prot
Match: SGSL_TRIAN (Seed lectin (Fragments) OS=Trichosanthes anguina PE=1 SV=2)

HSP 1 Score: 108.6 bits (270), Expect = 1.1e-22
Identity = 79/258 (30.62%), Postives = 123/258 (47.67%), Query Frame = 1

Query: 38  GRDGLCLQTA-PLSIYYTPTKLTDCDESDSDQLWAIVDDGTIRGDNDRNCLVPHSRGIKP 97
           GRD LC+  A  L+   +   L  C +   +Q W    DGT+R      CL  ++     
Sbjct: 269 GRDALCVDVAGALTSDGSRLILYPCGQQ-VNQKWTFHSDGTVRSLG--KCLATNN---SK 328

Query: 98  IGTNVVLVDCTKEIRSDMKWTKTNDGTIRHDE-SGLVLTGKQGQY---VSLEVDEGAPSQ 157
            G  VV+ DC+K    D+ W  +  GTI +     L LT  +      +++EV+  + SQ
Sbjct: 329 FGNLVVIYDCSKLAAEDISWDVSVGGTIMNPNYEDLALTSNKATRSTNLTMEVNTYSASQ 388

Query: 158 GWEATERPYPKVANIKWHNNLCLQFGDGEFSLGLSACSSKYNKSQRWVLYGDGTIRGNGD 217
           GW       P + +I   +++CL+  DG  ++ L  C     + Q W LY DGTIR + +
Sbjct: 389 GWRVGNYVQPIIGSIVGLDDMCLEATDGNTNMWLEECVPN-QREQSWALYSDGTIRVDDN 448

Query: 218 SNVCLTSLLSS-----SVVVSKCADMPQQRWGLAADNTIGHP-NTNLVMD-ARTFVRLSP 277
             +C+T+  S+      + +  C     QRW   AD +I  P N  L MD AR+ V L  
Sbjct: 449 RELCVTASSSTYDNWKVITILNCDGSNNQRWVFLADGSISTPGNQRLAMDVARSDVDLKK 508

Query: 278 LILVAKRTGTYSQQWIVY 284
           +IL  +  G  +QQW+++
Sbjct: 509 IIL-HRPHGDLNQQWVLF 518

BLAST of CmaCh04G005220 vs. Swiss-Prot
Match: RICI_RICCO (Ricin OS=Ricinus communis PE=1 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 9.5e-22
Identity = 79/255 (30.98%), Postives = 120/255 (47.06%), Query Frame = 1

Query: 36  LVGRDGLCLQTAPLSIYY-TPTKLTDC-DESDSDQLWAIVDDGTIRGDNDRNCLVPHSRG 95
           +VGR+GLC+       +     +L  C   +D++QLW +  D TIR +    CL  +  G
Sbjct: 327 IVGRNGLCVDVRDGRFHNGNAIQLWPCKSNTDANQLWTLKRDNTIRSNG--KCLTTY--G 386

Query: 96  IKPIGTNVVLVDCTKEIRSDMKWTKTNDGTIRHDESGLVL---TGKQGQYVSLEVDEGAP 155
             P G  V++ DC        +W   ++GTI +  S LVL   +G  G  ++++ +  A 
Sbjct: 387 YSP-GVYVMIYDCNTAATDATRWQIWDNGTIINPRSSLVLAATSGNSGTTLTVQTNIYAV 446

Query: 156 SQGWEATERPYPKVANIKWHNNLCLQFGDGEFSLGLSACSSKYNKSQRWVLYGDGTIRGN 215
           SQGW  T    P V  I     LCLQ   G+  + +  CSS+    Q+W LY DG+IR  
Sbjct: 447 SQGWLPTNNTQPFVTTIVGLYGLCLQANSGQ--VWIEDCSSE-KAEQQWALYADGSIRPQ 506

Query: 216 GDSNVCLTS---LLSSSVVVSKCADMPQ-QRWGLAADNTIGHPNTNLVMDARTFVRLSPL 275
            + + CLTS   +  + V +  C      QRW    D TI +  + LV+D R        
Sbjct: 507 QNRDNCLTSDSNIRETVVKILSCGPASSGQRWMFKNDGTILNLYSGLVLDVRASDPSLKQ 566

Query: 276 ILVAKRTGTYSQQWI 282
           I++    G  +Q W+
Sbjct: 567 IILYPLHGDPNQIWL 573

BLAST of CmaCh04G005220 vs. Swiss-Prot
Match: ABRA_ABRPR (Abrin-a OS=Abrus precatorius PE=1 SV=2)

HSP 1 Score: 102.8 bits (255), Expect = 6.2e-21
Identity = 75/254 (29.53%), Postives = 127/254 (50.00%), Query Frame = 1

Query: 38  GRDGLCLQTAPLSIYYTPTKLT--DC-DESDSDQLWAIVDDGTIRGDNDRNCLVPHSRGI 97
           GRDG+C+       Y+   ++    C D  + +QLW +  D TIR +    CL  +  G 
Sbjct: 281 GRDGMCVDVYDNG-YHNGNRIIMWKCKDRLEENQLWTLKSDKTIRSNG--KCLTTY--GY 340

Query: 98  KPIGTNVVLVDCTKEIRSDMKWTKTNDGTIRHDESGLVLTGKQ---GQYVSLEVDEGAPS 157
            P G+ V++ DCT  +     W   ++GTI + +S LVL+ +    G  ++++ +E    
Sbjct: 341 AP-GSYVMIYDCTSAVAEATYWEIWDNGTIINPKSALVLSAESSSMGGTLTVQTNEYLMR 400

Query: 158 QGWEATERPYPKVANIKWHNNLCLQFGDGEFSLGLSACSSKYNKSQRWVLYGDGTIRGNG 217
           QGW       P V +I  +++LC+Q   G  ++ ++ C S   K Q+W LY DG+IR   
Sbjct: 401 QGWRTGNNTSPFVTSISGYSDLCMQ-AQGS-NVWMADCDSN-KKEQQWALYTDGSIRSVQ 460

Query: 218 DSNVCLTS---LLSSSVVVSKCAD-MPQQRWGLAADNTIGHPNTNLVMDARTFVRLSPLI 277
           ++N CLTS      S++++  C++    QRW    D +I     ++VMD +        I
Sbjct: 461 NTNNCLTSKDHKQGSTILLMGCSNGWASQRWVFKNDGSIYSLYDDMVMDVKGSDPSLKQI 520

Query: 278 LVAKRTGTYSQQWI 282
           ++   TG  +Q W+
Sbjct: 521 ILWPYTGKPNQIWL 525

BLAST of CmaCh04G005220 vs. TrEMBL
Match: A0A0A0L756_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G219180 PE=4 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 8.0e-60
Identity = 140/293 (47.78%), Postives = 180/293 (61.43%), Query Frame = 1

Query: 5   RQVLLCMVVAFSLATHIGTTL----GYGAFGGRAHLVGRDGLCLQTAPLSIYYT----PT 64
           ++++L +VVAFSL TH+   +    GY    G  HLVGRDGLCL+ +P   +Y     PT
Sbjct: 2   KEIVLSIVVAFSLTTHLAMAVPSDYGYQVVPGATHLVGRDGLCLEMSPW--FYKGSNFPT 61

Query: 65  KLTDCDESDSD-QLWAIVDDGTIRGDNDRNCLVPHSRGIKPIGTNVVLVDCTKEIRSDMK 124
           +L+ C+E     QLW ++ D TIR  NDR CLV +   +  I TNVV+ +C KE  S+ K
Sbjct: 62  RLSPCNEQKKQTQLWTVLQDETIRPMNDRYCLVSYV-SVNFI-TNVVVSECAKEPHSNKK 121

Query: 125 WTKTNDGTIRHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKVANIKWHNNLCL 184
           W    D TI H  SG+VLTG    YV+++ ++ APSQ WEAT+   P VANIKW  NLCL
Sbjct: 122 WIHKEDRTIVHVGSGMVLTGNSN-YVTVQRNKNAPSQSWEATKSLTPMVANIKWLENLCL 181

Query: 185 QFGDGEFSLGLSACSSKYNKSQRWVLYGDGTIRGNGDSNVCLTSL--LSSSVVVSKCADM 244
           Q       + L  C+S+ NKSQ W LYGDGTIR + + N CLTS       V VSKC D 
Sbjct: 182 QSTKDSNYVQLDGCNSE-NKSQHWSLYGDGTIRKHVNRNYCLTSEQDFGRFVAVSKCEDK 241

Query: 245 PQQRWGLAA-DNTIGHPNTNLVMDART--FVRLSPLILVAKRTGTYSQQWIVY 284
           PQQRWGL A DNTI HPNT++VMD  +  F  L   ++   R GT +Q+WI+Y
Sbjct: 242 PQQRWGLGAEDNTINHPNTDMVMDVLSVPFDDLPLAVVTNHRDGTATQRWIIY 288

BLAST of CmaCh04G005220 vs. TrEMBL
Match: A0A0A0LAC2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G217140 PE=4 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 2.0e-58
Identity = 136/294 (46.26%), Postives = 172/294 (58.50%), Query Frame = 1

Query: 5   RQVLLCMVVAFSLATHI--------GTTLGYGAFGGRAHLVGRDGLCLQTAPLSIYYT-- 64
           ++++L ++VAFSL T +        G  +GYG   G  HLVGRDGLCL+   L  Y    
Sbjct: 2   KEIVLSIIVAFSLTTQLVIAVPPNYGYGVGYGGVPGATHLVGRDGLCLEMVLLRSYKPAG 61

Query: 65  ---PTKLTDCDESDSDQLWAIVDDGTIRGDNDRNCLVPHSRGIKPIGTNVVLVDCTKEIR 124
              PT+L  CDE    QLW +V DGTIR  NDR CL         +    V+ +C K   
Sbjct: 62  INFPTRLLPCDEKKQTQLWTVVGDGTIRPMNDRFCLAADV--FYGVINKAVVSECGKVSD 121

Query: 125 SDMKWTKTNDGTIRHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKVANIKWHN 184
           S+ +W   NDGTI   ES +VLTG    YV+L+  +  PSQ WE TE   P VANI+W N
Sbjct: 122 SNKQWIHKNDGTIALAESRMVLTG-DSDYVTLQSIKYTPSQSWEVTESLNPMVANIEWLN 181

Query: 185 NLCLQFGDGEFSLGLSACSSKYNKSQRWVLYGDGTIRGNGDSNVCLTS--LLSSSVVVSK 244
           NLCLQ  D   ++GL+ C++ Y K QRW LY DGTIR + + N CLTS      SVVVSK
Sbjct: 182 NLCLQSTDDSSNVGLNGCNT-YTKYQRWALYADGTIRQHVNRNYCLTSEQNYDRSVVVSK 241

Query: 245 CADMPQQRWGL-AADNTIGHPNTNLVMDARTFV-RLSPLILVAKR-TGTYSQQW 281
           C D PQQRW L A D+TI HPNTN+V+D    +   SPL++V     G+ SQ+W
Sbjct: 242 CEDKPQQRWSLNAKDSTIDHPNTNMVLDVLNVLDSASPLVIVTNHPDGSASQRW 291

BLAST of CmaCh04G005220 vs. TrEMBL
Match: A0A0A0L9D1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G217160 PE=4 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.2e-55
Identity = 133/295 (45.08%), Postives = 176/295 (59.66%), Query Frame = 1

Query: 5   RQVLLCMVVAFSLATHI--------GTTLGYGAFGGRAHLVGRDGLCLQTAP----LSIY 64
           ++++L ++VAFSL T +        G  +GYG   G  HLVGRDGLCL+ +P      I 
Sbjct: 2   KEIVLSIIVAFSLTTQLVIAVPPNYGYGVGYGGVPGATHLVGRDGLCLEMSPWYKPAGIN 61

Query: 65  YTPTKLTDCDESDSDQLWAIVDDGTIRGDNDRNCLVPHSRGIKPIGTNVVLVDCTKEIRS 124
           + PT+L+ CDE    QLW IV DGTIR  ND+ CL   +     +    V+ +C K    
Sbjct: 62  F-PTRLSPCDEKKQTQLWTIVGDGTIRPMNDKFCLA--AEVFYGVINKAVVSECGKVSDP 121

Query: 125 DMKWTKTNDGTIRHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKVANIKWHNN 184
           + KWT+ NDGTI   +S +VLTG    YV+L+ ++  PSQ WE TE     VANI+W NN
Sbjct: 122 NKKWTQKNDGTIALVDSRMVLTGDL-DYVTLQSNKYTPSQSWEVTESLNSMVANIEWLNN 181

Query: 185 LCLQFGDGEFSLGLSACSSKYNKSQRWVLYGDGTIRGNGDSNVCLTS--LLSSSVVVSKC 244
           LCLQ  D    +GL+ C++  NK QRW LY DGTIR + + N CLTS       VVVSKC
Sbjct: 182 LCLQSTDDSSHVGLNGCNTD-NKYQRWALYADGTIRQHVNKNYCLTSDQDFGRFVVVSKC 241

Query: 245 ADMPQQRWGL-AADNTIGHPNTNLVMDARTFVRLS-PLILVA-KRTGTYSQQWIV 283
            D PQQRW L A D TI HPNT++V+D  +    + P +LV  +R G+ SQ+W +
Sbjct: 242 EDKPQQRWSLDAKDYTIDHPNTDMVLDVFSVPDSTFPSVLVTNRRDGSASQRWTI 291

BLAST of CmaCh04G005220 vs. TrEMBL
Match: Q9SLV9_CUCSA (XSP30 OS=Cucumis sativus GN=XSP30 PE=2 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.2e-55
Identity = 133/295 (45.08%), Postives = 176/295 (59.66%), Query Frame = 1

Query: 5   RQVLLCMVVAFSLATHI--------GTTLGYGAFGGRAHLVGRDGLCLQTAP----LSIY 64
           ++++L ++VAFSL T +        G  +GYG   G  HLVGRDGLCL+ +P      I 
Sbjct: 2   KEIVLSIIVAFSLTTQLAIAVPPNYGYGVGYGGVPGATHLVGRDGLCLEMSPWYKPAGIN 61

Query: 65  YTPTKLTDCDESDSDQLWAIVDDGTIRGDNDRNCLVPHSRGIKPIGTNVVLVDCTKEIRS 124
           + PT+L+ CDE    QLW IV DGTIR  ND+ CL   +     +    V+ +C K    
Sbjct: 62  F-PTRLSPCDEKKQTQLWTIVGDGTIRPMNDKFCLA--AEVFYGVINKAVVSECGKVSDP 121

Query: 125 DMKWTKTNDGTIRHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKVANIKWHNN 184
           + KWT+ NDGTI   +S +VLTG    YV+L+ ++  PSQ WE TE     VANI+W NN
Sbjct: 122 NKKWTQKNDGTIALVDSRMVLTGDL-DYVTLQSNKYTPSQSWEVTESLNSMVANIEWLNN 181

Query: 185 LCLQFGDGEFSLGLSACSSKYNKSQRWVLYGDGTIRGNGDSNVCLTS--LLSSSVVVSKC 244
           LCLQ  D    +GL+ C++  NK QRW LY DGTIR + + N CLTS       VVVSKC
Sbjct: 182 LCLQSTDDSSHVGLNGCNTD-NKYQRWALYADGTIRQHVNKNYCLTSDQDFGRFVVVSKC 241

Query: 245 ADMPQQRWGL-AADNTIGHPNTNLVMDARTFVRLS-PLILVA-KRTGTYSQQWIV 283
            D PQQRW L A D TI HPNT++V+D  +    + P +LV  +R G+ SQ+W +
Sbjct: 242 EDKPQQRWSLDAKDYTIDHPNTDMVLDVFSVPDSTFPSVLVTNRRDGSASQRWTI 291

BLAST of CmaCh04G005220 vs. TrEMBL
Match: O04071_SAMNI (rRNA N-glycosidase OS=Sambucus nigra PE=2 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.2e-28
Identity = 88/248 (35.48%), Postives = 116/248 (46.77%), Query Frame = 1

Query: 38  GRDGLCLQTAP-LSIYYTPTKLTDCDESDSDQLWAIVDDGTIRGDNDRNCLVPHSRGIKP 97
           GRDGLC+     L+    P +L  C +  S Q W    DGTIR      CL         
Sbjct: 320 GRDGLCVDVRDGLAKDGNPVQLLSCGQQSSQQ-WTFRTDGTIRSLG--KCLTNSGDSS-- 379

Query: 98  IGTNVVLVDCTKEIRSDMKWTKTNDGTIRHDESGLVLTGKQ---GQYVSLEVDEGAPSQG 157
            G   ++ +C   IR   KW  + DGTI H  SGLVLT  Q   G  + L+ +  A SQG
Sbjct: 380 -GNYAMIYNCDTAIRDATKWVLSIDGTITHRLSGLVLTAPQAAQGTTLLLQKNIHAASQG 439

Query: 158 WEATERPYPKVANIKWHNNLCLQFGDGEFSLGLSACSSKYNKSQRWVLYGDGTIRGNGDS 217
           W       P V  I  +N +CL+       L L  C     + Q W LYGDGTIR N D 
Sbjct: 440 W-IVGNVVPLVTFIVGYNQMCLRANTQNNPLWLEDCVLN-RREQMWALYGDGTIRVNSDR 499

Query: 218 NVCLTSLLSSSVVVSKCADMPQQRWGLAADNTIGHPNTNLVMDARTFVRLSPLILVAKRT 277
           ++C++   S  +++ KC     QRW    + TI +PN  LVM+ R        I++   T
Sbjct: 500 SLCVSHSSSDLIIILKCQGSSNQRWVFNTNGTISNPNAKLVMEVRQSDVSLRQIIIYHPT 559

Query: 278 GTYSQQWI 282
           G  +QQWI
Sbjct: 560 GNLNQQWI 559

BLAST of CmaCh04G005220 vs. NCBI nr
Match: gi|659112133|ref|XP_008456079.1| (PREDICTED: nigrin b-like [Cucumis melo])

HSP 1 Score: 245.0 bits (624), Expect = 1.6e-61
Identity = 137/296 (46.28%), Postives = 184/296 (62.16%), Query Frame = 1

Query: 5   RQVLLCMVVAFSLATHIGTTL----GYGA-FGG---RAHLVGRDGLCLQTAPLSIYYT-- 64
           ++++L ++VAFSL TH+   +    GYG  +GG     HLVGRDGLCL+ +P   +Y   
Sbjct: 2   KEIVLSIIVAFSLTTHLAMAVPSNYGYGGEYGGVPRATHLVGRDGLCLEMSPW--FYRGN 61

Query: 65  --PTKLTDCDESDSD-QLWAIVDDGTIRGDNDRNCLVPHSRGIKPIGTNVVLVDCTKEIR 124
             PT+L+ CDE     QLW +V DGTIR  ND+ CLV     +  +    V+ +C KE  
Sbjct: 62  NFPTRLSPCDEHKKQTQLWTVVGDGTIRPMNDKFCLVADV--LVGLINKAVVRECAKEPN 121

Query: 125 SDMKWTKTNDGTIRHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKVANIKWHN 184
           S+ +WT   DGTI H ESG+VLTG    YV+L+ ++  PSQ WEATE   P +ANI+W N
Sbjct: 122 SNKRWTHKKDGTITHVESGMVLTG-DSDYVTLQSNKNTPSQSWEATESSTPMIANIEWLN 181

Query: 185 NLCLQFGDGEFSLGLSACSSKYNKSQRWVLYGDGTIRGNGDSNVCLTS--LLSSSVVVSK 244
           NLCLQ  D    +GL+ C ++ NK QRW LYGDGTIR N + N CLTS   L   +V+SK
Sbjct: 182 NLCLQSTDDSNHVGLNGC-NRENKDQRWALYGDGTIRQNVNRNYCLTSDEYLGRFIVMSK 241

Query: 245 CADMPQQRWGLAA-DNTIGHPNTNLVMDAR--TFVRLSPLILVAKRTGTYSQQWIV 283
           C D PQQRW L A D+TI HPN+++VMD     +   S  ++  +R G+ SQ+W +
Sbjct: 242 CEDKPQQRWNLGAKDSTIDHPNSDMVMDVLFVPYYDFSSAVVTNRRDGSASQRWTI 291

BLAST of CmaCh04G005220 vs. NCBI nr
Match: gi|449457105|ref|XP_004146289.1| (PREDICTED: nigrin b-like [Cucumis sativus])

HSP 1 Score: 238.8 bits (608), Expect = 1.2e-59
Identity = 140/293 (47.78%), Postives = 180/293 (61.43%), Query Frame = 1

Query: 5   RQVLLCMVVAFSLATHIGTTL----GYGAFGGRAHLVGRDGLCLQTAPLSIYYT----PT 64
           ++++L +VVAFSL TH+   +    GY    G  HLVGRDGLCL+ +P   +Y     PT
Sbjct: 2   KEIVLSIVVAFSLTTHLAMAVPSDYGYQVVPGATHLVGRDGLCLEMSPW--FYKGSNFPT 61

Query: 65  KLTDCDESDSD-QLWAIVDDGTIRGDNDRNCLVPHSRGIKPIGTNVVLVDCTKEIRSDMK 124
           +L+ C+E     QLW ++ D TIR  NDR CLV +   +  I TNVV+ +C KE  S+ K
Sbjct: 62  RLSPCNEQKKQTQLWTVLQDETIRPMNDRYCLVSYV-SVNFI-TNVVVSECAKEPHSNKK 121

Query: 125 WTKTNDGTIRHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKVANIKWHNNLCL 184
           W    D TI H  SG+VLTG    YV+++ ++ APSQ WEAT+   P VANIKW  NLCL
Sbjct: 122 WIHKEDRTIVHVGSGMVLTGNSN-YVTVQRNKNAPSQSWEATKSLTPMVANIKWLENLCL 181

Query: 185 QFGDGEFSLGLSACSSKYNKSQRWVLYGDGTIRGNGDSNVCLTSL--LSSSVVVSKCADM 244
           Q       + L  C+S+ NKSQ W LYGDGTIR + + N CLTS       V VSKC D 
Sbjct: 182 QSTKDSNYVQLDGCNSE-NKSQHWSLYGDGTIRKHVNRNYCLTSEQDFGRFVAVSKCEDK 241

Query: 245 PQQRWGLAA-DNTIGHPNTNLVMDART--FVRLSPLILVAKRTGTYSQQWIVY 284
           PQQRWGL A DNTI HPNT++VMD  +  F  L   ++   R GT +Q+WI+Y
Sbjct: 242 PQQRWGLGAEDNTINHPNTDMVMDVLSVPFDDLPLAVVTNHRDGTATQRWIIY 288

BLAST of CmaCh04G005220 vs. NCBI nr
Match: gi|778680239|ref|XP_004151509.2| (PREDICTED: nigrin b-like [Cucumis sativus])

HSP 1 Score: 234.2 bits (596), Expect = 2.8e-58
Identity = 136/294 (46.26%), Postives = 172/294 (58.50%), Query Frame = 1

Query: 5   RQVLLCMVVAFSLATHI--------GTTLGYGAFGGRAHLVGRDGLCLQTAPLSIYYT-- 64
           ++++L ++VAFSL T +        G  +GYG   G  HLVGRDGLCL+   L  Y    
Sbjct: 2   KEIVLSIIVAFSLTTQLVIAVPPNYGYGVGYGGVPGATHLVGRDGLCLEMVLLRSYKPAG 61

Query: 65  ---PTKLTDCDESDSDQLWAIVDDGTIRGDNDRNCLVPHSRGIKPIGTNVVLVDCTKEIR 124
              PT+L  CDE    QLW +V DGTIR  NDR CL         +    V+ +C K   
Sbjct: 62  INFPTRLLPCDEKKQTQLWTVVGDGTIRPMNDRFCLAADV--FYGVINKAVVSECGKVSD 121

Query: 125 SDMKWTKTNDGTIRHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKVANIKWHN 184
           S+ +W   NDGTI   ES +VLTG    YV+L+  +  PSQ WE TE   P VANI+W N
Sbjct: 122 SNKQWIHKNDGTIALAESRMVLTG-DSDYVTLQSIKYTPSQSWEVTESLNPMVANIEWLN 181

Query: 185 NLCLQFGDGEFSLGLSACSSKYNKSQRWVLYGDGTIRGNGDSNVCLTS--LLSSSVVVSK 244
           NLCLQ  D   ++GL+ C++ Y K QRW LY DGTIR + + N CLTS      SVVVSK
Sbjct: 182 NLCLQSTDDSSNVGLNGCNT-YTKYQRWALYADGTIRQHVNRNYCLTSEQNYDRSVVVSK 241

Query: 245 CADMPQQRWGL-AADNTIGHPNTNLVMDARTFV-RLSPLILVAKR-TGTYSQQW 281
           C D PQQRW L A D+TI HPNTN+V+D    +   SPL++V     G+ SQ+W
Sbjct: 242 CEDKPQQRWSLNAKDSTIDHPNTNMVLDVLNVLDSASPLVIVTNHPDGSASQRW 291

BLAST of CmaCh04G005220 vs. NCBI nr
Match: gi|700202446|gb|KGN57579.1| (hypothetical protein Csa_3G217160 [Cucumis sativus])

HSP 1 Score: 224.9 bits (572), Expect = 1.7e-55
Identity = 133/295 (45.08%), Postives = 176/295 (59.66%), Query Frame = 1

Query: 5   RQVLLCMVVAFSLATHI--------GTTLGYGAFGGRAHLVGRDGLCLQTAP----LSIY 64
           ++++L ++VAFSL T +        G  +GYG   G  HLVGRDGLCL+ +P      I 
Sbjct: 2   KEIVLSIIVAFSLTTQLVIAVPPNYGYGVGYGGVPGATHLVGRDGLCLEMSPWYKPAGIN 61

Query: 65  YTPTKLTDCDESDSDQLWAIVDDGTIRGDNDRNCLVPHSRGIKPIGTNVVLVDCTKEIRS 124
           + PT+L+ CDE    QLW IV DGTIR  ND+ CL   +     +    V+ +C K    
Sbjct: 62  F-PTRLSPCDEKKQTQLWTIVGDGTIRPMNDKFCLA--AEVFYGVINKAVVSECGKVSDP 121

Query: 125 DMKWTKTNDGTIRHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKVANIKWHNN 184
           + KWT+ NDGTI   +S +VLTG    YV+L+ ++  PSQ WE TE     VANI+W NN
Sbjct: 122 NKKWTQKNDGTIALVDSRMVLTGDL-DYVTLQSNKYTPSQSWEVTESLNSMVANIEWLNN 181

Query: 185 LCLQFGDGEFSLGLSACSSKYNKSQRWVLYGDGTIRGNGDSNVCLTS--LLSSSVVVSKC 244
           LCLQ  D    +GL+ C++  NK QRW LY DGTIR + + N CLTS       VVVSKC
Sbjct: 182 LCLQSTDDSSHVGLNGCNTD-NKYQRWALYADGTIRQHVNKNYCLTSDQDFGRFVVVSKC 241

Query: 245 ADMPQQRWGL-AADNTIGHPNTNLVMDARTFVRLS-PLILVA-KRTGTYSQQWIV 283
            D PQQRW L A D TI HPNT++V+D  +    + P +LV  +R G+ SQ+W +
Sbjct: 242 EDKPQQRWSLDAKDYTIDHPNTDMVLDVFSVPDSTFPSVLVTNRRDGSASQRWTI 291

BLAST of CmaCh04G005220 vs. NCBI nr
Match: gi|566006087|ref|NP_001274403.1| (uncharacterized protein LOC101221335 precursor [Cucumis sativus])

HSP 1 Score: 224.9 bits (572), Expect = 1.7e-55
Identity = 133/295 (45.08%), Postives = 176/295 (59.66%), Query Frame = 1

Query: 5   RQVLLCMVVAFSLATHI--------GTTLGYGAFGGRAHLVGRDGLCLQTAP----LSIY 64
           ++++L ++VAFSL T +        G  +GYG   G  HLVGRDGLCL+ +P      I 
Sbjct: 2   KEIVLSIIVAFSLTTQLAIAVPPNYGYGVGYGGVPGATHLVGRDGLCLEMSPWYKPAGIN 61

Query: 65  YTPTKLTDCDESDSDQLWAIVDDGTIRGDNDRNCLVPHSRGIKPIGTNVVLVDCTKEIRS 124
           + PT+L+ CDE    QLW IV DGTIR  ND+ CL   +     +    V+ +C K    
Sbjct: 62  F-PTRLSPCDEKKQTQLWTIVGDGTIRPMNDKFCLA--AEVFYGVINKAVVSECGKVSDP 121

Query: 125 DMKWTKTNDGTIRHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKVANIKWHNN 184
           + KWT+ NDGTI   +S +VLTG    YV+L+ ++  PSQ WE TE     VANI+W NN
Sbjct: 122 NKKWTQKNDGTIALVDSRMVLTGDL-DYVTLQSNKYTPSQSWEVTESLNSMVANIEWLNN 181

Query: 185 LCLQFGDGEFSLGLSACSSKYNKSQRWVLYGDGTIRGNGDSNVCLTS--LLSSSVVVSKC 244
           LCLQ  D    +GL+ C++  NK QRW LY DGTIR + + N CLTS       VVVSKC
Sbjct: 182 LCLQSTDDSSHVGLNGCNTD-NKYQRWALYADGTIRQHVNKNYCLTSDQDFGRFVVVSKC 241

Query: 245 ADMPQQRWGL-AADNTIGHPNTNLVMDARTFVRLS-PLILVA-KRTGTYSQQWIV 283
            D PQQRW L A D TI HPNT++V+D  +    + P +LV  +R G+ SQ+W +
Sbjct: 242 EDKPQQRWSLDAKDYTIDHPNTDMVLDVFSVPDSTFPSVLVTNRRDGSASQRWTI 291

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
RIP1_SAMNI3.6e-2936.22Ribosome-inactivating protein SNAI' OS=Sambucus nigra PE=1 SV=1[more]
ML3_VISAL1.2e-2430.98Beta-galactoside-specific lectin 3 OS=Viscum album PE=1 SV=2[more]
SGSL_TRIAN1.1e-2230.62Seed lectin (Fragments) OS=Trichosanthes anguina PE=1 SV=2[more]
RICI_RICCO9.5e-2230.98Ricin OS=Ricinus communis PE=1 SV=1[more]
ABRA_ABRPR6.2e-2129.53Abrin-a OS=Abrus precatorius PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0L756_CUCSA8.0e-6047.78Uncharacterized protein OS=Cucumis sativus GN=Csa_3G219180 PE=4 SV=1[more]
A0A0A0LAC2_CUCSA2.0e-5846.26Uncharacterized protein OS=Cucumis sativus GN=Csa_3G217140 PE=4 SV=1[more]
A0A0A0L9D1_CUCSA1.2e-5545.08Uncharacterized protein OS=Cucumis sativus GN=Csa_3G217160 PE=4 SV=1[more]
Q9SLV9_CUCSA1.2e-5545.08XSP30 OS=Cucumis sativus GN=XSP30 PE=2 SV=1[more]
O04071_SAMNI1.2e-2835.48rRNA N-glycosidase OS=Sambucus nigra PE=2 SV=1[more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
gi|659112133|ref|XP_008456079.1|1.6e-6146.28PREDICTED: nigrin b-like [Cucumis melo][more]
gi|449457105|ref|XP_004146289.1|1.2e-5947.78PREDICTED: nigrin b-like [Cucumis sativus][more]
gi|778680239|ref|XP_004151509.2|2.8e-5846.26PREDICTED: nigrin b-like [Cucumis sativus][more]
gi|700202446|gb|KGN57579.1|1.7e-5545.08hypothetical protein Csa_3G217160 [Cucumis sativus][more]
gi|566006087|ref|NP_001274403.1|1.7e-5545.08uncharacterized protein LOC101221335 precursor [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000772Ricin_B_lectin
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G005220.1CmaCh04G005220.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000772Ricin B, lectin domainPFAMPF00652Ricin_B_lectincoord: 36..154
score: 4.6E-15coord: 170..280
score: 5.0
IPR000772Ricin B, lectin domainSMARTSM00458ricin_3coord: 31..157
score: 2.7E-8coord: 161..283
score: 5.5
IPR000772Ricin B, lectin domainPROFILEPS50231RICIN_B_LECTINcoord: 171..283
score: 24.188coord: 33..157
score: 22
IPR000772Ricin B, lectin domainunknownSSF50370Ricin B-like lectinscoord: 36..155
score: 3.8E-19coord: 160..282
score: 5.57
NoneNo IPR availableGENE3DG3DSA:2.80.10.50coord: 36..155
score: 3.7E-18coord: 167..282
score: 1.2