Lsi05G001820 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G001820
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	rRNA N-glycosidase
Location	chr05 : 2626318 .. 2627196 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGAGAAATTGTGTTGAGCATTGTTGTGGCTTTCTCACTCACCACCCACCTTGTCATAGCATCGTCCTCAAATTATGGATATGGAGGATTCCCTGGAAGCACACATCTTGTAGGTCGAGATGGATTATGTTTAGAGGCATCTCCATGGTTTACCAAAGGTAATTATTTTTCAACTCGATTATCACCATGCGACGACGAACGTAGGATGAAACCAGCTCAATTATGGACAATCCTCCAAGACGGGACGATTCGAACGATGAATAATAGATATTGTTTGGTTTCTTACCTCCCTCTTGGTATGACCACAACCAATGTGATAGTAAGCTTATGTGAAAAGGAATCAAATCCTAACAAAATATGGAACCACAAGAAAGACGGGACCATAGTCCACGTCGAGTCCGGGATGGTTCTAACAGGAAAATTCGATAGCTATGTGACATTAGAAAGAAACGAAAATGTACCATCACAAAGCTGGGAAGTCACAGAAAATTTAACCCCAATGGTGGCGAACATCAAATGGCTTGACAACTTGTGTTTGCAATCTACGGAAGATTCGAATAATGTGGGGTTGGATGGATGTAATAGAGAAAATAAGAATCAAAGATGGGCATTATATGGAGATGGAACTATTCGACAAAATGTCAATAGAAACTATTGCTTGACTTCTAAAGAAGATTATGGTCATTATATAGCTGTGTCTAAATGTGAAGACATACCACAACAACGTTGGGGGCTTAGTGCAGAAGACAACACTATTAACCATCCAGCCAACATTGATATGGTCTTGGATGTGCTTGATGTGCCATTAAAGTATCCACTAGCGATCTTGATGAGCCATCGTGATGGAAGTCCTAGCCAGCGATGGATTATTTACTGA

mRNA sequence

ATGAGAGAAATTGTGTTGAGCATTGTTGTGGCTTTCTCACTCACCACCCACCTTGTCATAGCATCGTCCTCAAATTATGGATATGGAGGATTCCCTGGAAGCACACATCTTGTAGGTCGAGATGGATTATGTTTAGAGGCATCTCCATGGTTTACCAAAGGTAATTATTTTTCAACTCGATTATCACCATGCGACGACGAACGTAGGATGAAACCAGCTCAATTATGGACAATCCTCCAAGACGGGACGATTCGAACGATGAATAATAGATATTGTTTGGTTTCTTACCTCCCTCTTGGTATGACCACAACCAATGTGATAGTAAGCTTATGTGAAAAGGAATCAAATCCTAACAAAATATGGAACCACAAGAAAGACGGGACCATAGTCCACGTCGAGTCCGGGATGGTTCTAACAGGAAAATTCGATAGCTATGTGACATTAGAAAGAAACGAAAATGTACCATCACAAAGCTGGGAAGTCACAGAAAATTTAACCCCAATGGTGGCGAACATCAAATGGCTTGACAACTTGTGTTTGCAATCTACGGAAGATTCGAATAATGTGGGGTTGGATGGATGTAATAGAGAAAATAAGAATCAAAGATGGGCATTATATGGAGATGGAACTATTCGACAAAATGTCAATAGAAACTATTGCTTGACTTCTAAAGAAGATTATGGTCATTATATAGCTGTGTCTAAATGTGAAGACATACCACAACAACGTTGGGGGCTTAGTGCAGAAGACAACACTATTAACCATCCAGCCAACATTGATATGGTCTTGGATGTGCTTGATGTGCCATTAAAGTATCCACTAGCGATCTTGATGAGCCATCGTGATGGAAGTCCTAGCCAGCGATGGATTATTTACTGA

Coding sequence (CDS)

ATGAGAGAAATTGTGTTGAGCATTGTTGTGGCTTTCTCACTCACCACCCACCTTGTCATAGCATCGTCCTCAAATTATGGATATGGAGGATTCCCTGGAAGCACACATCTTGTAGGTCGAGATGGATTATGTTTAGAGGCATCTCCATGGTTTACCAAAGGTAATTATTTTTCAACTCGATTATCACCATGCGACGACGAACGTAGGATGAAACCAGCTCAATTATGGACAATCCTCCAAGACGGGACGATTCGAACGATGAATAATAGATATTGTTTGGTTTCTTACCTCCCTCTTGGTATGACCACAACCAATGTGATAGTAAGCTTATGTGAAAAGGAATCAAATCCTAACAAAATATGGAACCACAAGAAAGACGGGACCATAGTCCACGTCGAGTCCGGGATGGTTCTAACAGGAAAATTCGATAGCTATGTGACATTAGAAAGAAACGAAAATGTACCATCACAAAGCTGGGAAGTCACAGAAAATTTAACCCCAATGGTGGCGAACATCAAATGGCTTGACAACTTGTGTTTGCAATCTACGGAAGATTCGAATAATGTGGGGTTGGATGGATGTAATAGAGAAAATAAGAATCAAAGATGGGCATTATATGGAGATGGAACTATTCGACAAAATGTCAATAGAAACTATTGCTTGACTTCTAAAGAAGATTATGGTCATTATATAGCTGTGTCTAAATGTGAAGACATACCACAACAACGTTGGGGGCTTAGTGCAGAAGACAACACTATTAACCATCCAGCCAACATTGATATGGTCTTGGATGTGCTTGATGTGCCATTAAAGTATCCACTAGCGATCTTGATGAGCCATCGTGATGGAAGTCCTAGCCAGCGATGGATTATTTACTGA

Protein sequence

MREIVLSIVVAFSLTTHLVIASSSNYGYGGFPGSTHLVGRDGLCLEASPWFTKGNYFSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLPLGMTTTNVIVSLCEKESNPNKIWNHKKDGTIVHVESGMVLTGKFDSYVTLERNENVPSQSWEVTENLTPMVANIKWLDNLCLQSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQNVNRNYCLTSKEDYGHYIAVSKCEDIPQQRWGLSAEDNTINHPANIDMVLDVLDVPLKYPLAILMSHRDGSPSQRWIIY

Homology

[Close]

BLAST of Lsi05G001820 vs. Swiss-Prot
Match: NIGB_SAMNI (Nigrin b OS=Sambucus nigra PE=1 SV=2)

HSP 1 Score: 134.0 bits (336), Expect = 2.6e-30
Identity = 84/261 (32.18%), Postives = 136/261 (52.11%), Query Frame = 1


Query: 36  HLVGRDGLCLEASPWF-TKGNYFSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLV 95
           ++VGRDGLC++    + T G     +L PC  +R     Q WT   D TIR+M    C+ 
Sbjct: 311 NIVGRDGLCVDVRNGYDTDGT--PLQLWPCGTQRN----QRWTFDSDDTIRSMGK--CMT 370

Query: 96  SYLPLGMTT-TNVIVSLCEKESNPNKIWNHKKDGTIVHVESGMVLTGKFDSYVT---LER 155
           +    G+   +N+++  C   +     W    DG+I++  SG+V+T    +  T   LE 
Sbjct: 371 AN---GLNNGSNIVIFNCSTAAENAIKWEVPIDGSIINPSSGLVMTAPRAASRTILLLED 430

Query: 156 NENVPSQSWEVTENLTPMVANIKWLDNLCLQSTEDSNNVGLDGCNRENKNQRWALYGDGT 215
           N    SQ W VT N+ P+VA+I     +CLQS  ++N V ++ C   +  Q+WALYGD T
Sbjct: 431 NIYAASQGWTVTNNVKPIVASIVGYKEMCLQSNGENNGVWMEDCEATSLQQQWALYGDRT 490

Query: 216 IRQNVNRNYCLTSK-EDYGHYIAVSKCEDIPQQRWGLSAEDNTINHPANIDMVLDVLDVP 275
           IR N  R  C+T+   +    I + KC+ +P QRW  +++   +N  +   M +   +V 
Sbjct: 491 IRVNSTRGLCVTTNGYNSKDLIIILKCQGLPSQRWFFNSDGAIVNPKSRHVMDVRASNVS 550

Query: 276 LKYPLAILMSHRDGSPSQRWI 291
           L+    I++    G+P+Q+W+
Sbjct: 551 LR---EIIIFPATGNPNQQWV 557

[Close]

BLAST of Lsi05G001820 vs. Swiss-Prot
Match: RIP1_SAMNI (Ribosome-inactivating protein SNAI' OS=Sambucus nigra PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 8.3e-29
Identity = 84/259 (32.43%), Postives = 134/259 (51.74%), Query Frame = 1


Query: 39  GRDGLCLEASPWFTKGNYFSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLP 98
           GRDG C E      K      +LS C ++      Q WT   DGTI+++    CL     
Sbjct: 323 GRDGFCAEVKNGDEKDGT-PVQLSSCGEQSN----QQWTFSTDGTIQSLGK--CLT---- 382

Query: 99  LGMTTTNVIVSLCEKESNPNKIWNHKKDGTIVHVESGMVLTGKF---DSYVTLERNENVP 158
              T+++V++  C+     +  W    DGTI +  SG+VLT       + V+LE+N +  
Sbjct: 383 ---TSSSVMIYNCKVVPPESTKWVVSIDGTITNPRSGLVLTAPKAAEGTLVSLEKNVHAA 442

Query: 159 SQSWEVTENLTPMVANIKWLDNLCLQSTEDSNNVGLDGCNRENK---NQRWALYGDGTIR 218
            Q W +  N+ P+V  I   + +CL++   +N+V L  C+ ++    +Q+WALYGDGTIR
Sbjct: 443 RQGW-IVGNVEPLVTFIVGYEQMCLETNPGNNDVSLGDCSVKSASKVDQKWALYGDGTIR 502

Query: 219 QNVNRNYCLTSK-EDYGHYIAVSKCEDIPQQRWGLSAEDNTINHPANIDMVLDVLDVPLK 278
            N +R+ C+TS+ +     I + KC     QRW  + +    N  + + M +D  DVPL+
Sbjct: 503 VNNDRSLCVTSEGKSSNEPIIILKCLGWANQRWVFNTDGTISNPDSKLVMHVDQNDVPLR 562

Query: 279 YPLAILMSHRDGSPSQRWI 291
               I++SH  G+ +Q+WI
Sbjct: 563 ---KIILSHPSGTSNQQWI 563

[Close]

BLAST of Lsi05G001820 vs. Swiss-Prot
Match: SGSL_TRIAN (Seed lectin (Fragments) OS=Trichosanthes anguina PE=1 SV=2)

HSP 1 Score: 117.9 bits (294), Expect = 1.9e-25
Identity = 81/268 (30.22%), Postives = 130/268 (48.51%), Query Frame = 1


Query: 34  STHLVGRDGLCLEASPWFTKGNYFSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCL 93
           +T + GRD LC++ +   T        L PC  +   K    WT   DGT+R++    CL
Sbjct: 264 TTRISGRDALCVDVAGALTSDGS-RLILYPCGQQVNQK----WTFHSDGTVRSLGK--CL 323

Query: 94  VSYLPLGMTTTNVIVSLCEKESNPNKIWNHKKDGTIVHVE-SGMVLTGKF---DSYVTLE 153
            +          V++  C K +  +  W+    GTI++     + LT       + +T+E
Sbjct: 324 ATNN--SKFGNLVVIYDCSKLAAEDISWDVSVGGTIMNPNYEDLALTSNKATRSTNLTME 383

Query: 154 RNENVPSQSWEVTENLTPMVANIKWLDNLCLQSTEDSNNVGLDGCNRENKNQRWALYGDG 213
            N    SQ W V   + P++ +I  LD++CL++T+ + N+ L+ C    + Q WALY DG
Sbjct: 384 VNTYSASQGWRVGNYVQPIIGSIVGLDDMCLEATDGNTNMWLEECVPNQREQSWALYSDG 443

Query: 214 TIRQNVNRNYCLT---SKEDYGHYIAVSKCEDIPQQRWGLSAEDNTINHPANIDMVLDVL 273
           TIR + NR  C+T   S  D    I +  C+    QRW   A D +I+ P N  + +DV 
Sbjct: 444 TIRVDDNRELCVTASSSTYDNWKVITILNCDGSNNQRWVFLA-DGSISTPGNQRLAMDVA 503

Query: 274 --DVPLKYPLAILMSHRDGSPSQRWIIY 293
             DV LK    I++    G  +Q+W+++
Sbjct: 504 RSDVDLK---KIILHRPHGDLNQQWVLF 518

[Close]

BLAST of Lsi05G001820 vs. Swiss-Prot
Match: ABRC_ABRPR (Abrin-c OS=Abrus precatorius PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 2.5e-25
Identity = 83/259 (32.05%), Postives = 127/259 (49.03%), Query Frame = 1


Query: 39  GRDGLCLEASPWFTKGNYFSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLP 98
           GRDG+C++    +  G +   R+     + R++  QLWT+  D TIR+         Y P
Sbjct: 315 GRDGMCVDV---YDDGYHNGNRIIAWKCKDRLEENQLWTLKSDKTIRSNGKCLTTEGYAP 374

Query: 99  LGMTTTNVIVSLCEKESNPNKIWNHKKDGTIVHVESGMVLTGKFDSY---VTLERNENVP 158
                  V++  C         W    +GTI++ +S +VL+ +  S    +T++ NE + 
Sbjct: 375 ----GNYVMIYDCTSAVAEATYWEIWDNGTIINPKSALVLSAESSSMGGTLTVQTNEYLM 434

Query: 159 SQSWEVTENLTPMVANIKWLDNLCLQSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQNV 218
            Q W    N +P V +I    +LC+Q+    +NV L  C+   K Q+WALY DG+IR   
Sbjct: 435 RQGWRTGNNTSPFVTSISGYSDLCMQA--QGSNVWLADCDNNKKEQQWALYTDGSIRSVQ 494

Query: 219 NRNYCLTSKE-DYGHYIAVSKCED-IPQQRWGLSAEDNTINHPANIDMVLDV--LDVPLK 278
           N N CLTSK+   G  I +  C +    QRW L   D +I +  + DMV+DV   D  LK
Sbjct: 495 NTNNCLTSKDHKQGSPIVLMACSNGWASQRW-LFKNDGSI-YNLHDDMVMDVKRSDPSLK 554

Query: 279 YPLAILMSHRDGSPSQRWI 291
               I++    G P+Q W+
Sbjct: 555 ---EIILHPYHGKPNQIWL 559

[Close]

BLAST of Lsi05G001820 vs. Swiss-Prot
Match: ABRD_ABRPR (Abrin-d OS=Abrus precatorius PE=2 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.2e-24
Identity = 83/259 (32.05%), Postives = 126/259 (48.65%), Query Frame = 1


Query: 39  GRDGLCLEASPWFTKGNYFSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLP 98
           GRDG+C++    +  G +   R+     + R++  QLWT+  D TIR+         Y P
Sbjct: 281 GRDGMCVDV---YDDGYHNGNRIIAWKCKDRLEENQLWTLKSDLTIRSNGKCLTTEGYAP 340

Query: 99  LGMTTTNVIVSLCEKESNPNKIWNHKKDGTIVHVESGMVLTGKFDSY---VTLERNENVP 158
                  V++  C         W    +GTI++ +S +VL+ +  S    +T++ NE + 
Sbjct: 341 ----GNYVMIYDCTSAVAEATYWEIWDNGTIINPKSALVLSAESSSMGGTLTVQTNEYLM 400

Query: 159 SQSWEVTENLTPMVANIKWLDNLCLQSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQNV 218
            Q W    N +P V +I    +LC+Q+    +NV L  C+   K Q+WALY DG+IR   
Sbjct: 401 RQGWRTGNNTSPFVTSISGYSDLCMQA--QGSNVWLADCDNNKKEQQWALYTDGSIRSVQ 460

Query: 219 NRNYCLTSKE-DYGHYIAVSKCED-IPQQRWGLSAEDNTINHPANIDMVLDV--LDVPLK 278
           N N CLTSK+   G  I +  C +    QRW L   D +I +    DMV+DV   D  LK
Sbjct: 461 NTNNCLTSKDHKQGSPIVLMACSNGWASQRW-LFKNDGSI-YSLYDDMVMDVKGSDPSLK 520

Query: 279 YPLAILMSHRDGSPSQRWI 291
               I++    G P+Q W+
Sbjct: 521 ---QIILWPYTGKPNQIWL 525

[Close]

BLAST of Lsi05G001820 vs. TrEMBL
Match: A0A0A0L756_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G219180 PE=4 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 1.5e-114
Identity = 204/293 (69.62%), Postives = 243/293 (82.94%), Query Frame = 1


Query: 1   MREIVLSIVVAFSLTTHLVIASSSNYGYGGFPGSTHLVGRDGLCLEASPWFTKGNYFSTR 60
           M+EIVLSIVVAFSLTTHL +A  S+YGY   PG+THLVGRDGLCLE SPWF KG+ F TR
Sbjct: 1   MKEIVLSIVVAFSLTTHLAMAVPSDYGYQVVPGATHLVGRDGLCLEMSPWFYKGSNFPTR 60

Query: 61  LSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLPLGMTTTNVIVSLCEKESNPNKI 120
           LSPC++++  K  QLWT+LQD TIR MN+RYCLVSY+ +   T NV+VS C KE + NK 
Sbjct: 61  LSPCNEQK--KQTQLWTVLQDETIRPMNDRYCLVSYVSVNFIT-NVVVSECAKEPHSNKK 120

Query: 121 WNHKKDGTIVHVESGMVLTGKFDSYVTLERNENVPSQSWEVTENLTPMVANIKWLDNLCL 180
           W HK+D TIVHV SGMVLTG   +YVT++RN+N PSQSWE T++LTPMVANIKWL+NLCL
Sbjct: 121 WIHKEDRTIVHVGSGMVLTGN-SNYVTVQRNKNAPSQSWEATKSLTPMVANIKWLENLCL 180

Query: 181 QSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQNVNRNYCLTSKEDYGHYIAVSKCEDIP 240
           QST+DSN V LDGCN ENK+Q W+LYGDGTIR++VNRNYCLTS++D+G ++AVSKCED P
Sbjct: 181 QSTKDSNYVQLDGCNSENKSQHWSLYGDGTIRKHVNRNYCLTSEQDFGRFVAVSKCEDKP 240

Query: 241 QQRWGLSAEDNTINHPANIDMVLDVLDVPL-KYPLAILMSHRDGSPSQRWIIY 293
           QQRWGL AEDNTINHP N DMV+DVL VP    PLA++ +HRDG+ +QRWIIY
Sbjct: 241 QQRWGLGAEDNTINHP-NTDMVMDVLSVPFDDLPLAVVTNHRDGTATQRWIIY 288

[Close]

BLAST of Lsi05G001820 vs. TrEMBL
Match: A0A0A0L9D1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G217160 PE=4 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 5.9e-98
Identity = 186/297 (62.63%), Postives = 224/297 (75.42%), Query Frame = 1


Query: 1   MREIVLSIVVAFSLTTHLVIASSSNYGYG----GFPGSTHLVGRDGLCLEASPWFTK-GN 60
           M+EIVLSI+VAFSLTT LVIA   NYGYG    G PG+THLVGRDGLCLE SPW+   G 
Sbjct: 1   MKEIVLSIIVAFSLTTQLVIAVPPNYGYGVGYGGVPGATHLVGRDGLCLEMSPWYKPAGI 60

Query: 61  YFSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLPLGMTTTNVIVSLCEKES 120
            F TRLSPCD++   K  QLWTI+ DGTIR MN+++CL + +  G+     +VS C K S
Sbjct: 61  NFPTRLSPCDEK---KQTQLWTIVGDGTIRPMNDKFCLAAEVFYGVIN-KAVVSECGKVS 120

Query: 121 NPNKIWNHKKDGTIVHVESGMVLTGKFDSYVTLERNENVPSQSWEVTENLTPMVANIKWL 180
           +PNK W  K DGTI  V+S MVLTG  D YVTL+ N+  PSQSWEVTE+L  MVANI+WL
Sbjct: 121 DPNKKWTQKNDGTIALVDSRMVLTGDLD-YVTLQSNKYTPSQSWEVTESLNSMVANIEWL 180

Query: 181 DNLCLQSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQNVNRNYCLTSKEDYGHYIAVSK 240
           +NLCLQST+DS++VGL+GCN +NK QRWALY DGTIRQ+VN+NYCLTS +D+G ++ VSK
Sbjct: 181 NNLCLQSTDDSSHVGLNGCNTDNKYQRWALYADGTIRQHVNKNYCLTSDQDFGRFVVVSK 240

Query: 241 CEDIPQQRWGLSAEDNTINHPANIDMVLDVLDVP-LKYPLAILMSHRDGSPSQRWII 292
           CED PQQRW L A+D TI+HP N DMVLDV  VP   +P  ++ + RDGS SQRW I
Sbjct: 241 CEDKPQQRWSLDAKDYTIDHP-NTDMVLDVFSVPDSTFPSVLVTNRRDGSASQRWTI 291

[Close]

BLAST of Lsi05G001820 vs. TrEMBL
Match: Q9SLV9_CUCSA (XSP30 OS=Cucumis sativus GN=XSP30 PE=2 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 1.7e-97
Identity = 185/297 (62.29%), Postives = 223/297 (75.08%), Query Frame = 1


Query: 1   MREIVLSIVVAFSLTTHLVIASSSNYGYG----GFPGSTHLVGRDGLCLEASPWFTK-GN 60
           M+EIVLSI+VAFSLTT L IA   NYGYG    G PG+THLVGRDGLCLE SPW+   G 
Sbjct: 1   MKEIVLSIIVAFSLTTQLAIAVPPNYGYGVGYGGVPGATHLVGRDGLCLEMSPWYKPAGI 60

Query: 61  YFSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLPLGMTTTNVIVSLCEKES 120
            F TRLSPCD++   K  QLWTI+ DGTIR MN+++CL + +  G+     +VS C K S
Sbjct: 61  NFPTRLSPCDEK---KQTQLWTIVGDGTIRPMNDKFCLAAEVFYGVIN-KAVVSECGKVS 120

Query: 121 NPNKIWNHKKDGTIVHVESGMVLTGKFDSYVTLERNENVPSQSWEVTENLTPMVANIKWL 180
           +PNK W  K DGTI  V+S MVLTG  D YVTL+ N+  PSQSWEVTE+L  MVANI+WL
Sbjct: 121 DPNKKWTQKNDGTIALVDSRMVLTGDLD-YVTLQSNKYTPSQSWEVTESLNSMVANIEWL 180

Query: 181 DNLCLQSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQNVNRNYCLTSKEDYGHYIAVSK 240
           +NLCLQST+DS++VGL+GCN +NK QRWALY DGTIRQ+VN+NYCLTS +D+G ++ VSK
Sbjct: 181 NNLCLQSTDDSSHVGLNGCNTDNKYQRWALYADGTIRQHVNKNYCLTSDQDFGRFVVVSK 240

Query: 241 CEDIPQQRWGLSAEDNTINHPANIDMVLDVLDVP-LKYPLAILMSHRDGSPSQRWII 292
           CED PQQRW L A+D TI+HP N DMVLDV  VP   +P  ++ + RDGS SQRW I
Sbjct: 241 CEDKPQQRWSLDAKDYTIDHP-NTDMVLDVFSVPDSTFPSVLVTNRRDGSASQRWTI 291

[Close]

BLAST of Lsi05G001820 vs. TrEMBL
Match: A0A0A0LAC2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G217140 PE=4 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 3.1e-91
Identity = 182/297 (61.28%), Postives = 218/297 (73.40%), Query Frame = 1


Query: 1   MREIVLSIVVAFSLTTHLVIASSSNYGYG----GFPGSTHLVGRDGLCLEA---SPWFTK 60
           M+EIVLSI+VAFSLTT LVIA   NYGYG    G PG+THLVGRDGLCLE      +   
Sbjct: 1   MKEIVLSIIVAFSLTTQLVIAVPPNYGYGVGYGGVPGATHLVGRDGLCLEMVLLRSYKPA 60

Query: 61  GNYFSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLPLGMTTTNVIVSLCEK 120
           G  F TRL PCD++   K  QLWT++ DGTIR MN+R+CL + +  G+     +VS C K
Sbjct: 61  GINFPTRLLPCDEK---KQTQLWTVVGDGTIRPMNDRFCLAADVFYGVIN-KAVVSECGK 120

Query: 121 ESNPNKIWNHKKDGTIVHVESGMVLTGKFDSYVTLERNENVPSQSWEVTENLTPMVANIK 180
            S+ NK W HK DGTI   ES MVLTG  D YVTL+  +  PSQSWEVTE+L PMVANI+
Sbjct: 121 VSDSNKQWIHKNDGTIALAESRMVLTGDSD-YVTLQSIKYTPSQSWEVTESLNPMVANIE 180

Query: 181 WLDNLCLQSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQNVNRNYCLTSKEDYGHYIAV 240
           WL+NLCLQST+DS+NVGL+GCN   K QRWALY DGTIRQ+VNRNYCLTS+++Y   + V
Sbjct: 181 WLNNLCLQSTDDSSNVGLNGCNTYTKYQRWALYADGTIRQHVNRNYCLTSEQNYDRSVVV 240

Query: 241 SKCEDIPQQRWGLSAEDNTINHPANIDMVLDVLDV-PLKYPLAILMSHRDGSPSQRW 290
           SKCED PQQRW L+A+D+TI+HP N +MVLDVL+V     PL I+ +H DGS SQRW
Sbjct: 241 SKCEDKPQQRWSLNAKDSTIDHP-NTNMVLDVLNVLDSASPLVIVTNHPDGSASQRW 291

[Close]

BLAST of Lsi05G001820 vs. TrEMBL
Match: Q58A89_SAMRA (Lectin OS=Sambucus racemosa subsp. sieboldiana GN=LecSSA-b-3a PE=2 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.3e-28
Identity = 85/280 (30.36%), Postives = 149/280 (53.21%), Query Frame = 1


Query: 17  HLVIASSSNYGYGGFPGSTHLVGRDGLCLEA-SPWFTKGNYFSTRLSPCDDERRMKPAQL 76
           H ++   + Y   G P + +++GR+GLC++  + + T G     +L PC  +R     Q 
Sbjct: 3   HGLVDEDNKYN-DGEPITGNIIGRNGLCVDVRNGYSTDGT--PIQLWPCGRQRN----QQ 62

Query: 77  WTILQDGTIRTMNNRYCLVSYLPLGMTT-TNVIVSLCEKESNPNKIWNHKKDGTIVHVES 136
           WT  +DGTIR+M    C+ +    G+ + +N+++  C         W    DG+I+++ S
Sbjct: 63  WTFYEDGTIRSMGK--CMTAN---GLNSGSNIMIFNCSTAVENAIKWEVTIDGSIINLSS 122

Query: 137 GMVLTGKFDSYVT---LERNENVPSQSWEVTENLTPMVANIKWLDNLCLQSTEDSNNVGL 196
           G+V+T    +  T   LE N    SQ W V+ ++ P+VA+I     +CLQ+  ++N V +
Sbjct: 123 GLVMTAPSAASRTILLLENNIYAASQGWTVSNDVKPIVASIVGYKEMCLQANGENNGVWM 182

Query: 197 DGCNRENKNQRWALYGDGTIRQNVNRNYCLTSK-EDYGHYIAVSKCEDIPQQRWGLSAED 256
           + C   +  Q+WAL+GD TIR N +R  C+TS   +    I   KC+ +P QRW  ++  
Sbjct: 183 EDCEVTSLQQQWALFGDRTIRVNSSRGLCVTSNGYNSKDLIITLKCQGLPSQRWFFNSNG 242

Query: 257 NTINHPANIDMVLDVLDVPLKYPLAILMSHRDGSPSQRWI 291
             +N  + + M +   +V L+    I++S   G+P+Q+W+
Sbjct: 243 AIVNPNSTLVMDVRASNVSLR---EIIISPATGNPNQQWV 267

[Close]

BLAST of Lsi05G001820 vs. NCBI nr
Match: gi|449457105|ref|XP_004146289.1| (PREDICTED: nigrin b-like [Cucumis sativus])

HSP 1 Score: 420.6 bits (1080), Expect = 2.2e-114
Identity = 204/293 (69.62%), Postives = 243/293 (82.94%), Query Frame = 1


Query: 1   MREIVLSIVVAFSLTTHLVIASSSNYGYGGFPGSTHLVGRDGLCLEASPWFTKGNYFSTR 60
           M+EIVLSIVVAFSLTTHL +A  S+YGY   PG+THLVGRDGLCLE SPWF KG+ F TR
Sbjct: 1   MKEIVLSIVVAFSLTTHLAMAVPSDYGYQVVPGATHLVGRDGLCLEMSPWFYKGSNFPTR 60

Query: 61  LSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLPLGMTTTNVIVSLCEKESNPNKI 120
           LSPC++++  K  QLWT+LQD TIR MN+RYCLVSY+ +   T NV+VS C KE + NK 
Sbjct: 61  LSPCNEQK--KQTQLWTVLQDETIRPMNDRYCLVSYVSVNFIT-NVVVSECAKEPHSNKK 120

Query: 121 WNHKKDGTIVHVESGMVLTGKFDSYVTLERNENVPSQSWEVTENLTPMVANIKWLDNLCL 180
           W HK+D TIVHV SGMVLTG   +YVT++RN+N PSQSWE T++LTPMVANIKWL+NLCL
Sbjct: 121 WIHKEDRTIVHVGSGMVLTGN-SNYVTVQRNKNAPSQSWEATKSLTPMVANIKWLENLCL 180

Query: 181 QSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQNVNRNYCLTSKEDYGHYIAVSKCEDIP 240
           QST+DSN V LDGCN ENK+Q W+LYGDGTIR++VNRNYCLTS++D+G ++AVSKCED P
Sbjct: 181 QSTKDSNYVQLDGCNSENKSQHWSLYGDGTIRKHVNRNYCLTSEQDFGRFVAVSKCEDKP 240

Query: 241 QQRWGLSAEDNTINHPANIDMVLDVLDVPL-KYPLAILMSHRDGSPSQRWIIY 293
           QQRWGL AEDNTINHP N DMV+DVL VP    PLA++ +HRDG+ +QRWIIY
Sbjct: 241 QQRWGLGAEDNTINHP-NTDMVMDVLSVPFDDLPLAVVTNHRDGTATQRWIIY 288

[Close]

BLAST of Lsi05G001820 vs. NCBI nr
Match: gi|659112133|ref|XP_008456079.1| (PREDICTED: nigrin b-like [Cucumis melo])

HSP 1 Score: 399.4 bits (1025), Expect = 5.3e-108
Identity = 197/296 (66.55%), Postives = 234/296 (79.05%), Query Frame = 1


Query: 1   MREIVLSIVVAFSLTTHLVIASSSNYGYGG----FPGSTHLVGRDGLCLEASPWFTKGNY 60
           M+EIVLSI+VAFSLTTHL +A  SNYGYGG     P +THLVGRDGLCLE SPWF +GN 
Sbjct: 1   MKEIVLSIIVAFSLTTHLAMAVPSNYGYGGEYGGVPRATHLVGRDGLCLEMSPWFYRGNN 60

Query: 61  FSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLPLGMTTTNVIVSLCEKESN 120
           F TRLSPCD+ +  K  QLWT++ DGTIR MN+++CLV+ + +G+     +V  C KE N
Sbjct: 61  FPTRLSPCDEHK--KQTQLWTVVGDGTIRPMNDKFCLVADVLVGLIN-KAVVRECAKEPN 120

Query: 121 PNKIWNHKKDGTIVHVESGMVLTGKFDSYVTLERNENVPSQSWEVTENLTPMVANIKWLD 180
            NK W HKKDGTI HVESGMVLTG  D YVTL+ N+N PSQSWE TE+ TPM+ANI+WL+
Sbjct: 121 SNKRWTHKKDGTITHVESGMVLTGDSD-YVTLQSNKNTPSQSWEATESSTPMIANIEWLN 180

Query: 181 NLCLQSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQNVNRNYCLTSKEDYGHYIAVSKC 240
           NLCLQST+DSN+VGL+GCNRENK+QRWALYGDGTIRQNVNRNYCLTS E  G +I +SKC
Sbjct: 181 NLCLQSTDDSNHVGLNGCNRENKDQRWALYGDGTIRQNVNRNYCLTSDEYLGRFIVMSKC 240

Query: 241 EDIPQQRWGLSAEDNTINHPANIDMVLDVLDVP-LKYPLAILMSHRDGSPSQRWII 292
           ED PQQRW L A+D+TI+HP N DMV+DVL VP   +  A++ + RDGS SQRW I
Sbjct: 241 EDKPQQRWNLGAKDSTIDHP-NSDMVMDVLFVPYYDFSSAVVTNRRDGSASQRWTI 291

[Close]

BLAST of Lsi05G001820 vs. NCBI nr
Match: gi|700202446|gb|KGN57579.1| (hypothetical protein Csa_3G217160 [Cucumis sativus])

HSP 1 Score: 365.5 bits (937), Expect = 8.4e-98
Identity = 186/297 (62.63%), Postives = 224/297 (75.42%), Query Frame = 1


Query: 1   MREIVLSIVVAFSLTTHLVIASSSNYGYG----GFPGSTHLVGRDGLCLEASPWFTK-GN 60
           M+EIVLSI+VAFSLTT LVIA   NYGYG    G PG+THLVGRDGLCLE SPW+   G 
Sbjct: 1   MKEIVLSIIVAFSLTTQLVIAVPPNYGYGVGYGGVPGATHLVGRDGLCLEMSPWYKPAGI 60

Query: 61  YFSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLPLGMTTTNVIVSLCEKES 120
            F TRLSPCD++   K  QLWTI+ DGTIR MN+++CL + +  G+     +VS C K S
Sbjct: 61  NFPTRLSPCDEK---KQTQLWTIVGDGTIRPMNDKFCLAAEVFYGVIN-KAVVSECGKVS 120

Query: 121 NPNKIWNHKKDGTIVHVESGMVLTGKFDSYVTLERNENVPSQSWEVTENLTPMVANIKWL 180
           +PNK W  K DGTI  V+S MVLTG  D YVTL+ N+  PSQSWEVTE+L  MVANI+WL
Sbjct: 121 DPNKKWTQKNDGTIALVDSRMVLTGDLD-YVTLQSNKYTPSQSWEVTESLNSMVANIEWL 180

Query: 181 DNLCLQSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQNVNRNYCLTSKEDYGHYIAVSK 240
           +NLCLQST+DS++VGL+GCN +NK QRWALY DGTIRQ+VN+NYCLTS +D+G ++ VSK
Sbjct: 181 NNLCLQSTDDSSHVGLNGCNTDNKYQRWALYADGTIRQHVNKNYCLTSDQDFGRFVVVSK 240

Query: 241 CEDIPQQRWGLSAEDNTINHPANIDMVLDVLDVP-LKYPLAILMSHRDGSPSQRWII 292
           CED PQQRW L A+D TI+HP N DMVLDV  VP   +P  ++ + RDGS SQRW I
Sbjct: 241 CEDKPQQRWSLDAKDYTIDHP-NTDMVLDVFSVPDSTFPSVLVTNRRDGSASQRWTI 291

[Close]

BLAST of Lsi05G001820 vs. NCBI nr
Match: gi|566006087|ref|NP_001274403.1| (uncharacterized protein LOC101221335 precursor [Cucumis sativus])

HSP 1 Score: 364.0 bits (933), Expect = 2.4e-97
Identity = 185/297 (62.29%), Postives = 223/297 (75.08%), Query Frame = 1


Query: 1   MREIVLSIVVAFSLTTHLVIASSSNYGYG----GFPGSTHLVGRDGLCLEASPWFTK-GN 60
           M+EIVLSI+VAFSLTT L IA   NYGYG    G PG+THLVGRDGLCLE SPW+   G 
Sbjct: 1   MKEIVLSIIVAFSLTTQLAIAVPPNYGYGVGYGGVPGATHLVGRDGLCLEMSPWYKPAGI 60

Query: 61  YFSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLPLGMTTTNVIVSLCEKES 120
            F TRLSPCD++   K  QLWTI+ DGTIR MN+++CL + +  G+     +VS C K S
Sbjct: 61  NFPTRLSPCDEK---KQTQLWTIVGDGTIRPMNDKFCLAAEVFYGVIN-KAVVSECGKVS 120

Query: 121 NPNKIWNHKKDGTIVHVESGMVLTGKFDSYVTLERNENVPSQSWEVTENLTPMVANIKWL 180
           +PNK W  K DGTI  V+S MVLTG  D YVTL+ N+  PSQSWEVTE+L  MVANI+WL
Sbjct: 121 DPNKKWTQKNDGTIALVDSRMVLTGDLD-YVTLQSNKYTPSQSWEVTESLNSMVANIEWL 180

Query: 181 DNLCLQSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQNVNRNYCLTSKEDYGHYIAVSK 240
           +NLCLQST+DS++VGL+GCN +NK QRWALY DGTIRQ+VN+NYCLTS +D+G ++ VSK
Sbjct: 181 NNLCLQSTDDSSHVGLNGCNTDNKYQRWALYADGTIRQHVNKNYCLTSDQDFGRFVVVSK 240

Query: 241 CEDIPQQRWGLSAEDNTINHPANIDMVLDVLDVP-LKYPLAILMSHRDGSPSQRWII 292
           CED PQQRW L A+D TI+HP N DMVLDV  VP   +P  ++ + RDGS SQRW I
Sbjct: 241 CEDKPQQRWSLDAKDYTIDHP-NTDMVLDVFSVPDSTFPSVLVTNRRDGSASQRWTI 291

[Close]

BLAST of Lsi05G001820 vs. NCBI nr
Match: gi|778680239|ref|XP_004151509.2| (PREDICTED: nigrin b-like [Cucumis sativus])

HSP 1 Score: 343.2 bits (879), Expect = 4.5e-91
Identity = 182/297 (61.28%), Postives = 218/297 (73.40%), Query Frame = 1


Query: 1   MREIVLSIVVAFSLTTHLVIASSSNYGYG----GFPGSTHLVGRDGLCLEA---SPWFTK 60
           M+EIVLSI+VAFSLTT LVIA   NYGYG    G PG+THLVGRDGLCLE      +   
Sbjct: 1   MKEIVLSIIVAFSLTTQLVIAVPPNYGYGVGYGGVPGATHLVGRDGLCLEMVLLRSYKPA 60

Query: 61  GNYFSTRLSPCDDERRMKPAQLWTILQDGTIRTMNNRYCLVSYLPLGMTTTNVIVSLCEK 120
           G  F TRL PCD++   K  QLWT++ DGTIR MN+R+CL + +  G+     +VS C K
Sbjct: 61  GINFPTRLLPCDEK---KQTQLWTVVGDGTIRPMNDRFCLAADVFYGVIN-KAVVSECGK 120

Query: 121 ESNPNKIWNHKKDGTIVHVESGMVLTGKFDSYVTLERNENVPSQSWEVTENLTPMVANIK 180
            S+ NK W HK DGTI   ES MVLTG  D YVTL+  +  PSQSWEVTE+L PMVANI+
Sbjct: 121 VSDSNKQWIHKNDGTIALAESRMVLTGDSD-YVTLQSIKYTPSQSWEVTESLNPMVANIE 180

Query: 181 WLDNLCLQSTEDSNNVGLDGCNRENKNQRWALYGDGTIRQNVNRNYCLTSKEDYGHYIAV 240
           WL+NLCLQST+DS+NVGL+GCN   K QRWALY DGTIRQ+VNRNYCLTS+++Y   + V
Sbjct: 181 WLNNLCLQSTDDSSNVGLNGCNTYTKYQRWALYADGTIRQHVNRNYCLTSEQNYDRSVVV 240

Query: 241 SKCEDIPQQRWGLSAEDNTINHPANIDMVLDVLDV-PLKYPLAILMSHRDGSPSQRW 290
           SKCED PQQRW L+A+D+TI+HP N +MVLDVL+V     PL I+ +H DGS SQRW
Sbjct: 241 SKCEDKPQQRWSLNAKDSTIDHP-NTNMVLDVLNVLDSASPLVIVTNHPDGSASQRW 291

The following BLAST results are available for this feature:

BLAST of Lsi05G001820 vs. Swiss-Prot


Match Name	E-value	Identity	Description
NIGB_SAMNI	2.6e-30	32.18	Nigrin b OS=Sambucus nigra PE=1 SV=2	[more]
RIP1_SAMNI	8.3e-29	32.43	Ribosome-inactivating protein SNAI' OS=Sambucus nigra PE=1 SV=1	[more]
SGSL_TRIAN	1.9e-25	30.22	Seed lectin (Fragments) OS=Trichosanthes anguina PE=1 SV=2	[more]
ABRC_ABRPR	2.5e-25	32.05	Abrin-c OS=Abrus precatorius PE=1 SV=1	[more]
ABRD_ABRPR	1.2e-24	32.05	Abrin-d OS=Abrus precatorius PE=2 SV=1	[more]

BLAST of Lsi05G001820 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L756_CUCSA	1.5e-114	69.62	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G219180 PE=4 SV=1	[more]
A0A0A0L9D1_CUCSA	5.9e-98	62.63	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G217160 PE=4 SV=1	[more]
Q9SLV9_CUCSA	1.7e-97	62.29	XSP30 OS=Cucumis sativus GN=XSP30 PE=2 SV=1	[more]
A0A0A0LAC2_CUCSA	3.1e-91	61.28	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G217140 PE=4 SV=1	[more]
Q58A89_SAMRA	1.3e-28	30.36	Lectin OS=Sambucus racemosa subsp. sieboldiana GN=LecSSA-b-3a PE=2 SV=1	[more]

BLAST of Lsi05G001820 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of Lsi05G001820 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449457105\|ref\|XP_004146289.1\|	2.2e-114	69.62	PREDICTED: nigrin b-like [Cucumis sativus]	[more]
gi\|659112133\|ref\|XP_008456079.1\|	5.3e-108	66.55	PREDICTED: nigrin b-like [Cucumis melo]	[more]
gi\|700202446\|gb\|KGN57579.1\|	8.4e-98	62.63	hypothetical protein Csa_3G217160 [Cucumis sativus]	[more]
gi\|566006087\|ref\|NP_001274403.1\|	2.4e-97	62.29	uncharacterized protein LOC101221335 precursor [Cucumis sativus]	[more]
gi\|778680239\|ref\|XP_004151509.2\|	4.5e-91	61.28	PREDICTED: nigrin b-like [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000772	Ricin_B_lectin

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G001820.1	Lsi05G001820.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000772	Ricin B, lectin domain	PFAM	PF00652	Ricin_B_lectin	coord: 35..159 score: 6.4E-15coord: 176..289 score: 6.9
IPR000772	Ricin B, lectin domain	SMART	SM00458	ricin_3	coord: 31..162 score: 4.8E-7coord: 166..292 score: 2.2
IPR000772	Ricin B, lectin domain	PROFILE	PS50231	RICIN_B_LECTIN	coord: 165..292 score: 26.177coord: 31..162 score: 20
IPR000772	Ricin B, lectin domain	unknown	SSF50370	Ricin B-like lectins	coord: 167..291 score: 1.22E-25coord: 38..162 score: 2.04
None	No IPR available	GENE3D	G3DSA:2.80.10.50		coord: 171..291 score: 9.1E-21coord: 36..161 score: 2.1

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi05G001820	CmaCh04G005220	Cucurbita maxima (Rimu)	cmalsiB690
Lsi05G001820	CmoCh04G005610	Cucurbita moschata (Rifu)	cmolsiB683

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G001820	Cucurbita pepo (Zucchini)	cpelsiB177
Lsi05G001820	Cucurbita pepo (Zucchini)	cpelsiB369
Lsi05G001820	Wild cucumber (PI 183967)	cpilsiB209
Lsi05G001820	Cucumber (Chinese Long) v2	culsiB211
Lsi05G001820	Melon (DHL92) v3.5.1	lsimeB368
Lsi05G001820	Watermelon (Charleston Gray)	lsiwcgB332
Lsi05G001820	Watermelon (97103) v1	lsiwmB361
Lsi05G001820	Cucumber (Gy14) v2	cgyblsiB198
Lsi05G001820	Melon (DHL92) v3.6.1	lsimedB384
Lsi05G001820	Silver-seed gourd	carlsiB057
Lsi05G001820	Silver-seed gourd	carlsiB595
Lsi05G001820	Cucumber (Chinese Long) v3	cuclsiB230
Lsi05G001820	Watermelon (97103) v2	lsiwmbB329
Lsi05G001820	Wax gourd	lsiwgoB431
Lsi05G001820	Cucumber (Gy14) v1	cgylsiB003
Lsi05G001820	Cucurbita maxima (Rimu)	cmalsiB316
Lsi05G001820	Cucurbita moschata (Rifu)	cmolsiB304