Cla006431 (gene) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla006431
Type	gene
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Trypsin inhibitor 5 (AHRD V1 -*** ITR5_LUFCY); contains Interpro domain(s) IPR011052 Proteinase and amylase inhibitor
Location	Chr10 : 14911154 .. 14911408 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTGGAAGAGAGTTGGGTTGGTGGCTGTGGTAGGGATGCTGATAATGGCTGCTTTGGTGGAGGAGTGTGTAGCCATTGAAGCACAGTCTGTGGGAGGTTTGAATAAAAATGAATTCCCAAGAAAGATGATGAATGAAGATTATGATAATGTTAATAAAGGAAGAATGTGCCCTAGAATCCTTCAGGAGTGCACTACTGATGATGATTGCATGAATGACTGCATTTGCCTCTCTAATGGCTTTTGTGGTTGA

mRNA sequence

ATGGAGTGGAAGAGAGTTGGGTTGGTGGCTGTGGTAGGGATGCTGATAATGGCTGCTTTGGTGGAGGAGTGTGTAGCCATTGAAGCACAGTCTGTGGGAGGTTTGAATAAAAATGAATTCCCAAGAAAGATGATGAATGAAGATTATGATAATGTTAATAAAGGAAGAATGTGCCCTAGAATCCTTCAGGAGTGCACTACTGATGATGATTGCATGAATGACTGCATTTGCCTCTCTAATGGCTTTTGTGGTTGA

Coding sequence (CDS)

ATGGAGTGGAAGAGAGTTGGGTTGGTGGCTGTGGTAGGGATGCTGATAATGGCTGCTTTGGTGGAGGAGTGTGTAGCCATTGAAGCACAGTCTGTGGGAGGTTTGAATAAAAATGAATTCCCAAGAAAGATGATGAATGAAGATTATGATAATGTTAATAAAGGAAGAATGTGCCCTAGAATCCTTCAGGAGTGCACTACTGATGATGATTGCATGAATGACTGCATTTGCCTCTCTAATGGCTTTTGTGGTTGA

Protein sequence

MEWKRVGLVAVVGMLIMAALVEECVAIEAQSVGGLNKNEFPRKMMNEDYDNVNKGRMCPRILQECTTDDDCMNDCICLSNGFCG

Homology

[Close]

BLAST of Cla006431 vs. Swiss-Prot
Match: ITR5_LUFAE (Trypsin inhibitor 5 OS=Luffa aegyptiaca PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 5.5e-10
Identity = 31/68 (45.59%), Postives = 39/68 (57.35%), Query Frame = 1


Query: 17 MAALVEECVAIEAQSVGGLNKNEFPRKMMNEDYDNVNKGRMCPRILQECTTDDDCMNDCI 76
          MA++ E    +E   +     N+ PRK+M+  +     G +CPRIL  C TDDDCM DC 
Sbjct: 1  MASVAESSGVVEVIELISDGGNDLPRKIMSGRH-----GGICPRILMPCKTDDDCMLDCR 60

Query: 77 CLSNGFCG 85
          CLSNG+CG
Sbjct: 61 CLSNGYCG 63

[Close]

BLAST of Cla006431 vs. Swiss-Prot
Match: ITR3_LUFAE (Trypsin inhibitor 3 OS=Luffa aegyptiaca PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 2.6e-07
Identity = 20/29 (68.97%), Postives = 23/29 (79.31%), Query Frame = 1


Query: 56 RMCPRILQECTTDDDCMNDCICLSNGFCG 85
          R+CPRIL EC++D DC+ +CICL NGFCG
Sbjct: 1  RICPRILMECSSDSDCLAECICLENGFCG 29

[Close]

BLAST of Cla006431 vs. Swiss-Prot
Match: ITR1_LUFAE (Trypsin inhibitor 1 OS=Luffa aegyptiaca PE=1 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 1.3e-06
Identity = 19/29 (65.52%), Postives = 22/29 (75.86%), Query Frame = 1


Query: 56 RMCPRILQECTTDDDCMNDCICLSNGFCG 85
          R+CPRIL EC++D DC+ +CICL  GFCG
Sbjct: 1  RICPRILMECSSDSDCLAECICLEQGFCG 29

[Close]

BLAST of Cla006431 vs. Swiss-Prot
Match: ITR3_CUCMC (Trypsin inhibitor 3 OS=Cucumis melo var. conomon PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 4.8e-06
Identity = 18/29 (62.07%), Postives = 20/29 (68.97%), Query Frame = 1


Query: 56 RMCPRILQECTTDDDCMNDCICLSNGFCG 85
          RMCP+IL +C  D DC+ DC+CL  GFCG
Sbjct: 2  RMCPKILMKCKQDSDCLLDCVCLKEGFCG 30

[Close]

BLAST of Cla006431 vs. Swiss-Prot
Match: ITR4_CYCPE (Trypsin inhibitor 4 OS=Cyclanthera pedata PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 4.8e-06
Identity = 19/29 (65.52%), Postives = 19/29 (65.52%), Query Frame = 1


Query: 56 RMCPRILQECTTDDDCMNDCICLSNGFCG 85
          R+CPRIL EC  D DC+  CIC  NGFCG
Sbjct: 1  RICPRILMECKADSDCLAQCICQENGFCG 29

[Close]

BLAST of Cla006431 vs. TrEMBL
Match: A0A0A0KT01_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G000805 PE=3 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 1.7e-29
Identity = 64/85 (75.29%), Postives = 74/85 (87.06%), Query Frame = 1


Query: 1  MEWKRVGLVAVVGMLIMAALVEEC-VAIEAQSVGGLNKNEFPRKMMNEDYDNVNKGRMCP 60
          MEWK++G++AVVGML+MAALVE+C  AIE++SV GLNKNE PRKMMNE+       RMCP
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEET------RMCP 60

Query: 61 RILQECTTDDDCMNDCICLSNGFCG 85
          RIL+ECTTDDDCMNDCICLSNGFCG
Sbjct: 61 RILEECTTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of Cla006431 vs. TrEMBL
Match: A0A0A0KY34_CUCSA (Trypsin inhibitor 1 OS=Cucumis sativus GN=Csa_4G000810 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 2.3e-07
Identity = 36/89 (40.45%), Postives = 49/89 (55.06%), Query Frame = 1


Query: 1  MEWKRVGLVAVVGMLIMAALVEECVAIEAQSVG-GLNKN--EFPRKMMNEDYDNVNKGRM 60
          MEWK++ LVA++GML+MA   E        SVG G+++   +     +NE   N+ K   
Sbjct: 1  MEWKKIALVAMMGMLLMATFTE--------SVGLGVDEEIIQLVSDGVNEYSQNIGKDTA 60

Query: 61 --CPRILQECTTDDDCMNDCICLSNGFCG 85
            CPRIL +C TD DC   C C   G+CG
Sbjct: 61 PGCPRILMKCKTDSDCYPGCTCKPMGYCG 81

[Close]

BLAST of Cla006431 vs. NCBI nr
Match: gi|778697386|ref|XP_011654311.1| (PREDICTED: uncharacterized protein LOC105435333 [Cucumis sativus])

HSP 1 Score: 136.3 bits (342), Expect = 2.4e-29
Identity = 64/85 (75.29%), Postives = 74/85 (87.06%), Query Frame = 1


Query: 1   MEWKRVGLVAVVGMLIMAALVEEC-VAIEAQSVGGLNKNEFPRKMMNEDYDNVNKGRMCP 60
           MEWK++G++AVVGML+MAALVE+C  AIE++SV GLNKNE PRKMMNE+       RMCP
Sbjct: 126 MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEET------RMCP 185

Query: 61  RILQECTTDDDCMNDCICLSNGFCG 85
           RIL+ECTTDDDCMNDCICLSNGFCG
Sbjct: 186 RILEECTTDDDCMNDCICLSNGFCG 204

[Close]

BLAST of Cla006431 vs. NCBI nr
Match: gi|778697386|ref|XP_011654311.1| (PREDICTED: uncharacterized protein LOC105435333 [Cucumis sativus])

HSP 1 Score: 134.0 bits (336), Expect = 1.2e-28
Identity = 63/84 (75.00%), Postives = 73/84 (86.90%), Query Frame = 1


Query: 1  MEWKRVGLVAVVGMLIMAALVEEC-VAIEAQSVGGLNKNEFPRKMMNEDYDNVNKGRMCP 60
          MEWK++G++AVVGML+MAALVE+C  AIE++SV GLNKNE PRKMMNE+       RMCP
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEET------RMCP 60

Query: 61 RILQECTTDDDCMNDCICLSNGFC 84
          RIL+ECTTDDDCMNDCICLSNGFC
Sbjct: 61 RILEECTTDDDCMNDCICLSNGFC 78

HSP 2 Score: 136.3 bits (342), Expect = 2.4e-29
Identity = 64/85 (75.29%), Postives = 74/85 (87.06%), Query Frame = 1


Query: 1  MEWKRVGLVAVVGMLIMAALVEEC-VAIEAQSVGGLNKNEFPRKMMNEDYDNVNKGRMCP 60
          MEWK++G++AVVGML+MAALVE+C  AIE++SV GLNKNE PRKMMNE+       RMCP
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEET------RMCP 60

Query: 61 RILQECTTDDDCMNDCICLSNGFCG 85
          RIL+ECTTDDDCMNDCICLSNGFCG
Sbjct: 61 RILEECTTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of Cla006431 vs. NCBI nr
Match: gi|462426|sp|P34950.1|ITR5_LUFAE (RecName: Full=Trypsin inhibitor 5; AltName: Full=TGT-II; AltName: Full=Trypsin inhibitor II; Flags: Precursor)

HSP 1 Score: 64.7 bits (156), Expect = 8.8e-08
Identity = 31/68 (45.59%), Postives = 41/68 (60.29%), Query Frame = 1


Query: 17 MAALVEECVAIEAQSVGGLNKNEFPRKMMNEDYDNVNKGRMCPRILQECTTDDDCMNDCI 76
          MA++ E    +E   +     N+ PRK+M+  +     G +CPRIL  C TDDDCM DC 
Sbjct: 1  MASVAESSGVVEVIELISDGGNDLPRKIMSGRH-----GGICPRILMPCKTDDDCMLDCR 60

Query: 77 CLSNGFCG 85
          CLSNG+CG
Sbjct: 61 CLSNGYCG 63

[Close]

BLAST of Cla006431 vs. NCBI nr
Match: gi|700197603|gb|KGN52761.1| (Trypsin inhibitor 1 [Cucumis sativus])

HSP 1 Score: 62.8 bits (151), Expect = 3.4e-07
Identity = 36/89 (40.45%), Postives = 49/89 (55.06%), Query Frame = 1


Query: 1  MEWKRVGLVAVVGMLIMAALVEECVAIEAQSVG-GLNKN--EFPRKMMNEDYDNVNKGRM 60
          MEWK++ LVA++GML+MA   E        SVG G+++   +     +NE   N+ K   
Sbjct: 1  MEWKKIALVAMMGMLLMATFTE--------SVGLGVDEEIIQLVSDGVNEYSQNIGKDTA 60

Query: 61 --CPRILQECTTDDDCMNDCICLSNGFCG 85
            CPRIL +C TD DC   C C   G+CG
Sbjct: 61 PGCPRILMKCKTDSDCYPGCTCKPMGYCG 81

The following BLAST results are available for this feature:

BLAST of Cla006431 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ITR5_LUFAE	5.5e-10	45.59	Trypsin inhibitor 5 OS=Luffa aegyptiaca PE=1 SV=1	[more]
ITR3_LUFAE	2.6e-07	68.97	Trypsin inhibitor 3 OS=Luffa aegyptiaca PE=1 SV=1	[more]
ITR1_LUFAE	1.3e-06	65.52	Trypsin inhibitor 1 OS=Luffa aegyptiaca PE=1 SV=1	[more]
ITR3_CUCMC	4.8e-06	62.07	Trypsin inhibitor 3 OS=Cucumis melo var. conomon PE=1 SV=1	[more]
ITR4_CYCPE	4.8e-06	65.52	Trypsin inhibitor 4 OS=Cyclanthera pedata PE=1 SV=1	[more]

BLAST of Cla006431 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KT01_CUCSA	1.7e-29	75.29	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G000805 PE=3 SV=1	[more]
A0A0A0KY34_CUCSA	2.3e-07	40.45	Trypsin inhibitor 1 OS=Cucumis sativus GN=Csa_4G000810 PE=3 SV=1	[more]

BLAST of Cla006431 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778697386\|ref\|XP_011654311.1\|	2.4e-29	75.29	PREDICTED: uncharacterized protein LOC105435333 [Cucumis sativus]	[more]
gi\|778697386\|ref\|XP_011654311.1\|	1.2e-28	75.00	PREDICTED: uncharacterized protein LOC105435333 [Cucumis sativus]	[more]
gi\|462426\|sp\|P34950.1\|ITR5_LUFAE	8.8e-08	45.59	RecName: Full=Trypsin inhibitor 5; AltName: Full=TGT-II; AltName: Full=Trypsin i...	[more]
gi\|700197603\|gb\|KGN52761.1\|	3.4e-07	40.45	Trypsin inhibitor 1 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000737	Prot_inh_squash
IPR011052	Proteinase_amylase_inhib_sf

Vocabulary: **Molecular Function**
Term	Definition
GO:0004867	serine-type endopeptidase inhibitor activity
GO:0004866	endopeptidase inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0008150	biological_process
biological_process	GO:0010466	negative regulation of peptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity
molecular_function	GO:0030414	peptidase inhibitor activity

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
WMU40393	watermelon EST collection version 2.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla006431	Cla006431.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
WMU40393	WMU40393	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 56..84 score: 7.2
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 58..77 scor
IPR011052	Proteinase/amylase inhibitor domain	unknown	SSF57027	Plant inhibitors of proteinases and amylases	coord: 54..84 score: 4.55
None	No IPR available	SMART	SM00286	PTI_1	coord: 56..84 score: 5.3

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cla006431	ClCG10G010350	Watermelon (Charleston Gray)	wcgwmB051
Cla006431	CSPI04G00300	Wild cucumber (PI 183967)	cpiwmB291
Cla006431	Cucsa.097530	Cucumber (Gy14) v1	cgywmB115
Cla006431	CmaCh16G003170	Cucurbita maxima (Rimu)	cmawmB293
Cla006431	CmoCh16G003360	Cucurbita moschata (Rifu)	cmowmB287
Cla006431	Lsi03G010190	Bottle gourd (USVL1VR-Ls)	lsiwmB240
Cla006431	Cp4.1LG14g04630	Cucurbita pepo (Zucchini)	cpewmB204
Cla006431	CsGy4G000280	Cucumber (Gy14) v2	cgybwmB269
Cla006431	MELO3C018005.2	Melon (DHL92) v3.6.1	medwmB483
Cla006431	CsaV3_4G000290	Cucumber (Chinese Long) v3	cucwmB300
Cla006431	Bhi11G000936	Wax gourd	wgowmB007

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cla006431	Cucumber (Gy14) v2	cgybwmB276
Cla006431	Cucumber (Gy14) v2	cgybwmB538
Cla006431	Melon (DHL92) v3.6.1	medwmB214
Cla006431	Silver-seed gourd	carwmB0287
Cla006431	Silver-seed gourd	carwmB0366
Cla006431	Cucumber (Chinese Long) v3	cucwmB309
Cla006431	Cucumber (Chinese Long) v3	cucwmB602
Cla006431	Watermelon (97103) v2	wmwmbB063
Cla006431	Watermelon (97103) v2	wmwmbB051
Cla006431	Watermelon (97103) v1	wmwmB054
Cla006431	Cucumber (Gy14) v1	cgywmB421
Cla006431	Cucurbita maxima (Rimu)	cmawmB653
Cla006431	Cucurbita moschata (Rifu)	cmowmB478
Cla006431	Cucurbita moschata (Rifu)	cmowmB648
Cla006431	Wild cucumber (PI 183967)	cpiwmB299
Cla006431	Wild cucumber (PI 183967)	cpiwmB594
Cla006431	Melon (DHL92) v3.5.1	mewmB217
Cla006431	Melon (DHL92) v3.5.1	mewmB496
Cla006431	Melon (DHL92) v3.5.1	mewmB497
Cla006431	Watermelon (Charleston Gray)	wcgwmB176
Cla006431	Cucumber (Chinese Long) v2	cuwmB290
Cla006431	Cucumber (Chinese Long) v2	cuwmB575
Cla006431	Cucurbita pepo (Zucchini)	cpewmB382
Cla006431	Bottle gourd (USVL1VR-Ls)	lsiwmB245