CSPI04G00300 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI04G00300
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionTrypsin inhibitor 1
LocationChr4 : 183790 .. 184072 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GATTAAGAAGAGAAAAAAAGAAAGAAAGAAAGGAATTGAAAAAATGGAGTGGAAGAAAATTGGAATGTTGGCTGTGGTGGGGATGCTGGTGATGGCAGCTTTGGTGGAGGATTGTCATGCAGCCATTGAATCAGAGTCTGTGAAAGGTTTGAATAAAAATGAATTGCCAAGAAAGATGATGAATGAAGAAACTAGAATGTGCCCTAGAATCCTTGAAGAGTGCACTACGGACGATGATTGCATGAATGATTGCATTTGCCTCTCCAATGGCTTTTGCGGTTGA

mRNA sequence

ATGGAGTGGAAGAAAATTGGAATGTTGGCTGTGGTGGGGATGCTGGTGATGGCAGCTTTGGTGGAGGATTGTCATGCAGCCATTGAATCAGAGTCTGTGAAAGGTTTGAATAAAAATGAATTGCCAAGAAAGATGATGAATGAAGAAACTAGAATGTGCCCTAGAATCCTTGAAGAGTGCACTACGGACGATGATTGCATGAATGATTGCATTTGCCTCTCCAATGGCTTTTGCGGTTGA

Coding sequence (CDS)

ATGGAGTGGAAGAAAATTGGAATGTTGGCTGTGGTGGGGATGCTGGTGATGGCAGCTTTGGTGGAGGATTGTCATGCAGCCATTGAATCAGAGTCTGTGAAAGGTTTGAATAAAAATGAATTGCCAAGAAAGATGATGAATGAAGAAACTAGAATGTGCCCTAGAATCCTTGAAGAGTGCACTACGGACGATGATTGCATGAATGATTGCATTTGCCTCTCCAATGGCTTTTGCGGTTGA
Homology
[Close]
BLAST of CSPI04G00300 vs. Swiss-Prot
Match: ITR5_LUFAE (Trypsin inhibitor 5 OS=Luffa aegyptiaca PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.3e-08
Identity = 27/42 (64.29%), Postives = 32/42 (76.19%), Query Frame = 1 

Query: 39 NELPRKMMN-EETRMCPRILEECTTDDDCMNDCICLSNGFCG 80
          N+LPRK+M+     +CPRIL  C TDDDCM DC CLSNG+CG
Sbjct: 22 NDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLSNGYCG 63

[Close]
BLAST of CSPI04G00300 vs. Swiss-Prot
Match: ITR3_LUFAE (Trypsin inhibitor 3 OS=Luffa aegyptiaca PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 7.1e-07
Identity = 20/29 (68.97%), Postives = 25/29 (86.21%), Query Frame = 1 

Query: 51 RMCPRILEECTTDDDCMNDCICLSNGFCG 80
          R+CPRIL EC++D DC+ +CICL NGFCG
Sbjct: 1  RICPRILMECSSDSDCLAECICLENGFCG 29

[Close]
BLAST of CSPI04G00300 vs. Swiss-Prot
Match: ITR1_TRIKI (Trypsin inhibitor 1 OS=Trichosanthes kirilowii PE=3 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 7.1e-07
Identity = 31/66 (46.97%), Postives = 36/66 (54.55%), Query Frame = 1 

Query: 17 MAALVEDCHAAIESESVKGL---NKNELPRKMMNEETRMCPRILEECTTDDDCMNDCICL 76
          MAA VE   A   ++ V  L     NE   KMM E    CPRIL  C  +DDC+  C CL
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMM-EGVVACPRILMPCKVNDDCLRGCKCL 60

Query: 77 SNGFCG 80
          SNG+CG
Sbjct: 61 SNGYCG 65

[Close]
BLAST of CSPI04G00300 vs. Swiss-Prot
Match: ITR1_LUFAE (Trypsin inhibitor 1 OS=Luffa aegyptiaca PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 3.5e-06
Identity = 19/29 (65.52%), Postives = 24/29 (82.76%), Query Frame = 1 

Query: 51 RMCPRILEECTTDDDCMNDCICLSNGFCG 80
          R+CPRIL EC++D DC+ +CICL  GFCG
Sbjct: 1  RICPRILMECSSDSDCLAECICLEQGFCG 29

[Close]
BLAST of CSPI04G00300 vs. TrEMBL
Match: A0A0A0KT01_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G000805 PE=3 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 1.9e-38
Identity = 79/79 (100.00%), Postives = 79/79 (100.00%), Query Frame = 1 

Query: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRMCPRILEEC 60
          MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRMCPRILEEC
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRMCPRILEEC 60

Query: 61 TTDDDCMNDCICLSNGFCG 80
          TTDDDCMNDCICLSNGFCG
Sbjct: 61 TTDDDCMNDCICLSNGFCG 79

[Close]
BLAST of CSPI04G00300 vs. TrEMBL
Match: A0A0A0KY34_CUCSA (Trypsin inhibitor 1 OS=Cucumis sativus GN=Csa_4G000810 PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 7.7e-08
Identity = 31/81 (38.27%), Postives = 47/81 (58.02%), Query Frame = 1 

Query: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNK--NELPRKMMNEETRMCPRILE 60
          MEWKKI ++A++GML+MA   E     ++ E ++ ++   NE  + +  +    CPRIL 
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60

Query: 61 ECTTDDDCMNDCICLSNGFCG 80
          +C TD DC   C C   G+CG
Sbjct: 61 KCKTDSDCYPGCTCKPMGYCG 81

[Close]
BLAST of CSPI04G00300 vs. NCBI nr
Match: gi|778697386|ref|XP_011654311.1| (PREDICTED: uncharacterized protein LOC105435333 [Cucumis sativus])

HSP 1 Score: 166.0 bits (419), Expect = 2.7e-38
Identity = 79/79 (100.00%), Postives = 79/79 (100.00%), Query Frame = 1 

Query: 1   MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRMCPRILEEC 60
           MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRMCPRILEEC
Sbjct: 126 MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRMCPRILEEC 185

Query: 61  TTDDDCMNDCICLSNGFCG 80
           TTDDDCMNDCICLSNGFCG
Sbjct: 186 TTDDDCMNDCICLSNGFCG 204

[Close]
BLAST of CSPI04G00300 vs. NCBI nr
Match: gi|700197602|gb|KGN52760.1| (hypothetical protein Csa_4G000805 [Cucumis sativus])

HSP 1 Score: 166.0 bits (419), Expect = 2.7e-38
Identity = 79/79 (100.00%), Postives = 79/79 (100.00%), Query Frame = 1 

Query: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRMCPRILEEC 60
          MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRMCPRILEEC
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRMCPRILEEC 60

Query: 61 TTDDDCMNDCICLSNGFCG 80
          TTDDDCMNDCICLSNGFCG
Sbjct: 61 TTDDDCMNDCICLSNGFCG 79

[Close]
BLAST of CSPI04G00300 vs. NCBI nr
Match: gi|700197603|gb|KGN52761.1| (Trypsin inhibitor 1 [Cucumis sativus])

HSP 1 Score: 64.3 bits (155), Expect = 1.1e-07
Identity = 31/81 (38.27%), Postives = 47/81 (58.02%), Query Frame = 1 

Query: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNK--NELPRKMMNEETRMCPRILE 60
          MEWKKI ++A++GML+MA   E     ++ E ++ ++   NE  + +  +    CPRIL 
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60

Query: 61 ECTTDDDCMNDCICLSNGFCG 80
          +C TD DC   C C   G+CG
Sbjct: 61 KCKTDSDCYPGCTCKPMGYCG 81

[Close]
BLAST of CSPI04G00300 vs. NCBI nr
Match: gi|462426|sp|P34950.1|ITR5_LUFAE (RecName: Full=Trypsin inhibitor 5; AltName: Full=TGT-II; AltName: Full=Trypsin inhibitor II; Flags: Precursor)

HSP 1 Score: 60.1 bits (144), Expect = 2.1e-06
Identity = 27/42 (64.29%), Postives = 32/42 (76.19%), Query Frame = 1 

Query: 39 NELPRKMMN-EETRMCPRILEECTTDDDCMNDCICLSNGFCG 80
          N+LPRK+M+     +CPRIL  C TDDDCM DC CLSNG+CG
Sbjct: 22 NDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLSNGYCG 63

The following BLAST results are available for this feature:
BLAST of CSPI04G00300 vs. Swiss-Prot
Match NameE-valueIdentityDescription
ITR5_LUFAE1.3e-0864.29Trypsin inhibitor 5 OS=Luffa aegyptiaca PE=1 SV=1[more]
ITR3_LUFAE7.1e-0768.97Trypsin inhibitor 3 OS=Luffa aegyptiaca PE=1 SV=1[more]
ITR1_TRIKI7.1e-0746.97Trypsin inhibitor 1 OS=Trichosanthes kirilowii PE=3 SV=1[more]
ITR1_LUFAE3.5e-0665.52Trypsin inhibitor 1 OS=Luffa aegyptiaca PE=1 SV=1[more]
BLAST of CSPI04G00300 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KT01_CUCSA1.9e-38100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_4G000805 PE=3 SV=1[more]
A0A0A0KY34_CUCSA7.7e-0838.27Trypsin inhibitor 1 OS=Cucumis sativus GN=Csa_4G000810 PE=3 SV=1[more]
BLAST of CSPI04G00300 vs. TAIR10
Match NameE-valueIdentityDescription
BLAST of CSPI04G00300 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778697386|ref|XP_011654311.1|2.7e-38100.00PREDICTED: uncharacterized protein LOC105435333 [Cucumis sativus][more]
gi|700197602|gb|KGN52760.1|2.7e-38100.00hypothetical protein Csa_4G000805 [Cucumis sativus][more]
gi|700197603|gb|KGN52761.1|1.1e-0738.27Trypsin inhibitor 1 [Cucumis sativus][more]
gi|462426|sp|P34950.1|ITR5_LUFAE2.1e-0664.29RecName: Full=Trypsin inhibitor 5; AltName: Full=TGT-II; AltName: Full=Trypsin i... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000737Prot_inh_squash
IPR011052Proteinase_amylase_inhib_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004867serine-type endopeptidase inhibitor activity
GO:0004866endopeptidase inhibitor activity
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004867 serine-type endopeptidase inhibitor activity
molecular_function GO:0004866 endopeptidase inhibitor activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G00300.1CSPI04G00300.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000737Proteinase inhibitor I7, squashPFAMPF00299Squashcoord: 51..79
score: 8.9
IPR000737Proteinase inhibitor I7, squashPROSITEPS00286SQUASH_INHIBITORcoord: 53..72
scor
IPR011052Proteinase/amylase inhibitor domainunknownSSF57027Plant inhibitors of proteinases and amylasescoord: 48..79
score: 2.36
NoneNo IPR availableSMARTSM00286PTI_1coord: 51..79
score: 6.6

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CSPI04G00300Cla006431Watermelon (97103) v1cpiwmB291
CSPI04G00300ClCG10G010350Watermelon (Charleston Gray)cpiwcgB280
CSPI04G00300Lsi03G010190Bottle gourd (USVL1VR-Ls)cpilsiB274
CSPI04G00300CsaV3_4G000290Cucumber (Chinese Long) v3cpicucB213
CSPI04G00300Bhi11G000936Wax gourdcpiwgoB358
CSPI04G00300CsGy4G000280Cucumber (Gy14) v2cgybcpiB168
CSPI04G00300Carg19685Silver-seed gourdcarcpiB0328
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CSPI04G00300Cucumber (Chinese Long) v2cpicuB182
CSPI04G00300Cucumber (Chinese Long) v2cpicuB188
CSPI04G00300Cucumber (Chinese Long) v2cpicuB207
CSPI04G00300Melon (DHL92) v3.5.1cpimeB276
CSPI04G00300Melon (DHL92) v3.5.1cpimeB309
CSPI04G00300Melon (DHL92) v3.5.1cpimeB310
CSPI04G00300Cucurbita pepo (Zucchini)cpecpiB221
CSPI04G00300Cucurbita pepo (Zucchini)cpecpiB399
CSPI04G00300Melon (DHL92) v3.6.1cpimedB301
CSPI04G00300Cucumber (Gy14) v2cgybcpiB179
CSPI04G00300Silver-seed gourdcarcpiB0407
CSPI04G00300Cucumber (Chinese Long) v3cpicucB224
CSPI04G00300Watermelon (97103) v2cpiwmbB278
CSPI04G00300Watermelon (97103) v2cpiwmbB296
CSPI04G00300Wild cucumber (PI 183967)cpicpiB151
CSPI04G00300Wild cucumber (PI 183967)cpicpiB169
CSPI04G00300Cucumber (Gy14) v1cgycpiB522
CSPI04G00300Cucurbita maxima (Rimu)cmacpiB338
CSPI04G00300Cucurbita maxima (Rimu)cmacpiB715
CSPI04G00300Cucurbita moschata (Rifu)cmocpiB329
CSPI04G00300Cucurbita moschata (Rifu)cmocpiB709