Carg19685 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg19685
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Trypsin inhibitor 3
Location	Cucurbita_argyrosperma_scaffold_133 : 150896 .. 151321 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGCAAAACGAGATGACACAAATGGGCATTGCCAGCCAGATGCAATACACATTCTTATCATTCAAGTTTCAACGTCAAATTGGGAGCAAAATCTCCTCTTTCTCTTCTATAAAATGGTACCCAAACAGACAAGAGGTACTAACAAAAAGACCTGAAATGGGTAGCATGAAGATTGTCGCGGTGGCTCTAATCGCAATTTTGCTGGTAGCAACGTTGTCGTCGGCTGCCTATGCCATCGACGACGACTCGATGGACCTTGTCTTCGACGGTCGAGACATCAATCATGGAGTCCCAAGAAAGATCATGAAATGGGGAGTGCTTAATCATGAAGAAAGGGTATGTCCCAAAATCCTCATGGAATGCAAAAAGGACTCTGATTGCTTAGCTGAGTGTATCTGTCTCGAGCATGGATATTGTGGCTAA

mRNA sequence

ATGTGGCAAAACGAGATGACACAAATGGGCATTGCCAGCCAGATGCAATACACATTCTTATCATTCAAGTTTCAACGTCAAATTGGGAGCAAAATCTCCTCTTTCTCTTCTATAAAATGGTACCCAAACAGACAAGAGGTACTAACAAAAAGACCTGAAATGGGTAGCATGAAGATTGTCGCGGTGGCTCTAATCGCAATTTTGCTGGTAGCAACGTTGTCGTCGGCTGCCTATGCCATCGACGACGACTCGATGGACCTTGTCTTCGACGGTCGAGACATCAATCATGGAGTCCCAAGAAAGATCATGAAATGGGGAGTGCTTAATCATGAAGAAAGGGTATGTCCCAAAATCCTCATGGAATGCAAAAAGGACTCTGATTGCTTAGCTGAGTGTATCTGTCTCGAGCATGGATATTGTGGCTAA

Coding sequence (CDS)

ATGTGGCAAAACGAGATGACACAAATGGGCATTGCCAGCCAGATGCAATACACATTCTTATCATTCAAGTTTCAACGTCAAATTGGGAGCAAAATCTCCTCTTTCTCTTCTATAAAATGGTACCCAAACAGACAAGAGGTACTAACAAAAAGACCTGAAATGGGTAGCATGAAGATTGTCGCGGTGGCTCTAATCGCAATTTTGCTGGTAGCAACGTTGTCGTCGGCTGCCTATGCCATCGACGACGACTCGATGGACCTTGTCTTCGACGGTCGAGACATCAATCATGGAGTCCCAAGAAAGATCATGAAATGGGGAGTGCTTAATCATGAAGAAAGGGTATGTCCCAAAATCCTCATGGAATGCAAAAAGGACTCTGATTGCTTAGCTGAGTGTATCTGTCTCGAGCATGGATATTGTGGCTAA

Protein sequence

MWQNEMTQMGIASQMQYTFLSFKFQRQIGSKISSFSSIKWYPNRQEVLTKRPEMGSMKIVAVALIAILLVATLSSAAYAIDDDSMDLVFDGRDINHGVPRKIMKWGVLNHEERVCPKILMECKKDSDCLAECICLEHGYCG

Homology

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BLAST of Carg19685 vs. NCBI nr
Match: XP_022922566.1 (probable polygalacturonase At3g15720 [Cucurbita moschata])

HSP 1 Score: 157.1 bits (396), Expect = 4.3e-35
Identity = 78/80 (97.50%), Postives = 79/80 (98.75%), Query Frame = 0


Query: 54  MGSMKIVAVALIAILLVATLSSAAYAIDDDSMDLVFDGRDINHGVPRKIMKWGVLNHEER 113
           MGSMKIVAVALIAILLVATLSSAAYAIDDDSMDLVFDGRDINH VPRKIMKWGVLNHEER
Sbjct: 1   MGSMKIVAVALIAILLVATLSSAAYAIDDDSMDLVFDGRDINHRVPRKIMKWGVLNHEER 60

Query: 114 VCPKILMECKKDSDCLAECI 134
           VCPKILMECKKDSDCLAEC+
Sbjct: 61  VCPKILMECKKDSDCLAECL 80

[Close]

BLAST of Carg19685 vs. NCBI nr
Match: XP_023552846.1 (probable polygalacturonase At3g15720 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 146.0 bits (367), Expect = 9.8e-32
Identity = 74/78 (94.87%), Postives = 76/78 (97.44%), Query Frame = 0


Query: 54  MGSMKIVAVALIAILLVATLSSAAYAIDDDSMDLVFDGRDINHGVPRKIMKWGVLNHEER 113
           MGSMK VAVALIA++LVAT SSAAYAIDDDSMDLVFDGRDINHGVPRKIMKWGVLNHEER
Sbjct: 1   MGSMKNVAVALIAMMLVAT-SSAAYAIDDDSMDLVFDGRDINHGVPRKIMKWGVLNHEER 60

Query: 114 VCPKILMECKKDSDCLAE 132
           VCPKILMECKKDSDCLAE
Sbjct: 61  VCPKILMECKKDSDCLAE 77

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BLAST of Carg19685 vs. NCBI nr
Match: KGN52764.1 (hypothetical protein Csa_4G000835 [Cucumis sativus])

HSP 1 Score: 89.7 bits (221), Expect = 8.4e-15
Identity = 54/98 (55.10%), Postives = 68/98 (69.39%), Query Frame = 0


Query: 54  MGSMKIVAVALIA--ILLVATLSSAAYAIDD-DSMDLVFDGR-----DINHGVPRKIM-K 113
           M S KIV VA++A  I+ VA  SSA++ +DD D + LV DGR     D+N+G PRK+M K
Sbjct: 1   MESKKIVMVAIVATIIVQVAMSSSASFVVDDKDWIKLVSDGRAQADQDMNNGCPRKMMIK 60

Query: 114 WGVLNHEERVCPKILMECKKDSDCLAECICLEH-GYCG 142
            GV   EE +CP+ILM+CK DSDCL  C+CLEH  YCG
Sbjct: 61  GGVFKEEEMMCPRILMKCKHDSDCLPGCVCLEHIEYCG 98

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BLAST of Carg19685 vs. NCBI nr
Match: XP_011654312.1 (PREDICTED: trypsin inhibitor 1-like [Cucumis sativus])

HSP 1 Score: 82.4 bits (202), Expect = 1.3e-12
Identity = 42/68 (61.76%), Postives = 49/68 (72.06%), Query Frame = 0


Query: 81  DDDSMDLVFDGR-----DINHGVPRKIM-KWGVLNHEERVCPKILMECKKDSDCLAECIC 140
           D DS+ LV DGR     D+N+G PRK+M K GV   EE VCPKILM+CK DSDCL +C+C
Sbjct: 31  DKDSIKLVTDGRAQADQDMNNGCPRKMMIKGGVFKEEEMVCPKILMKCKHDSDCLLDCVC 90

Query: 141 LEH-GYCG 142
           LE  GYCG
Sbjct: 91  LEDIGYCG 98

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BLAST of Carg19685 vs. NCBI nr
Match: P10293.1 (RecName: Full=Trypsin inhibitor 3; AltName: Full=CPTI-III; AltName: Full=Trypsin inhibitor III; Contains: RecName: Full=Trypsin inhibitor 2; AltName: Full=CPTI-II; AltName: Full=Trypsin inhibitor II)

HSP 1 Score: 77.4 bits (189), Expect = 4.3e-11
Identity = 32/32 (100.00%), Postives = 32/32 (100.00%), Query Frame = 0


Query: 110 HEERVCPKILMECKKDSDCLAECICLEHGYCG 142
           HEERVCPKILMECKKDSDCLAECICLEHGYCG
Sbjct: 1   HEERVCPKILMECKKDSDCLAECICLEHGYCG 32

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BLAST of Carg19685 vs. Swiss-Prot
Match: sp|P10293|ITR3_CUCPE (Trypsin inhibitor 3 OS=Cucurbita pepo OX=3663 PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.4e-13
Identity = 32/32 (100.00%), Postives = 32/32 (100.00%), Query Frame = 0


Query: 110 HEERVCPKILMECKKDSDCLAECICLEHGYCG 142
           HEERVCPKILMECKKDSDCLAECICLEHGYCG
Sbjct: 1   HEERVCPKILMECKKDSDCLAECICLEHGYCG 32

[Close]

BLAST of Carg19685 vs. Swiss-Prot
Match: sp|P07853|ITR4_CUCMA (Trypsin inhibitor 4 OS=Cucurbita maxima OX=3661 PE=1 SV=2)

HSP 1 Score: 74.3 bits (181), Expect = 1.2e-12
Identity = 29/32 (90.62%), Postives = 32/32 (100.00%), Query Frame = 0


Query: 110 HEERVCPKILMECKKDSDCLAECICLEHGYCG 142
           HEERVCP+ILM+CKKDSDCLAEC+CLEHGYCG
Sbjct: 1   HEERVCPRILMKCKKDSDCLAECVCLEHGYCG 32

[Close]

BLAST of Carg19685 vs. Swiss-Prot
Match: sp|P01074|ITR1_CUCMA (Trypsin inhibitor 1 OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 5.0e-11
Identity = 27/29 (93.10%), Postives = 29/29 (100.00%), Query Frame = 0


Query: 113 RVCPKILMECKKDSDCLAECICLEHGYCG 142
           RVCP+ILMECKKDSDCLAEC+CLEHGYCG
Sbjct: 1   RVCPRILMECKKDSDCLAECVCLEHGYCG 29

[Close]

BLAST of Carg19685 vs. Swiss-Prot
Match: sp|P83398|ITR7_CYCPE (Trypsin inhibitor 7 OS=Cyclanthera pedata OX=198836 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 2.1e-09
Identity = 24/29 (82.76%), Postives = 28/29 (96.55%), Query Frame = 0


Query: 113 RVCPKILMECKKDSDCLAECICLEHGYCG 142
           R+CP+ILM+CKKDSDCLAECIC EHG+CG
Sbjct: 2   RICPRILMKCKKDSDCLAECICQEHGFCG 30

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BLAST of Carg19685 vs. Swiss-Prot
Match: sp|P83397|ITR6_CYCPE (Trypsin inhibitor 6 OS=Cyclanthera pedata OX=198836 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 2.7e-09
Identity = 24/29 (82.76%), Postives = 28/29 (96.55%), Query Frame = 0


Query: 113 RVCPKILMECKKDSDCLAECICLEHGYCG 142
           R+CP+ILM+CKKDSDCLAECIC EHG+CG
Sbjct: 2   RICPRILMKCKKDSDCLAECICEEHGFCG 30

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BLAST of Carg19685 vs. TrEMBL
Match: tr|A0A0A0KV23|A0A0A0KV23_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000835 PE=3 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 5.5e-15
Identity = 54/98 (55.10%), Postives = 68/98 (69.39%), Query Frame = 0


Query: 54  MGSMKIVAVALIA--ILLVATLSSAAYAIDD-DSMDLVFDGR-----DINHGVPRKIM-K 113
           M S KIV VA++A  I+ VA  SSA++ +DD D + LV DGR     D+N+G PRK+M K
Sbjct: 1   MESKKIVMVAIVATIIVQVAMSSSASFVVDDKDWIKLVSDGRAQADQDMNNGCPRKMMIK 60

Query: 114 WGVLNHEERVCPKILMECKKDSDCLAECICLEH-GYCG 142
            GV   EE +CP+ILM+CK DSDCL  C+CLEH  YCG
Sbjct: 61  GGVFKEEEMMCPRILMKCKHDSDCLPGCVCLEHIEYCG 98

[Close]

BLAST of Carg19685 vs. TrEMBL
Match: tr|J3R9Z5|J3R9Z5_MOMCO (Two inhibitor peptide topologies 3 OS=Momordica cochinchinensis OX=3674 GN=TIPTOP3 PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 5.0e-08
Identity = 41/91 (45.05%), Postives = 52/91 (57.14%), Query Frame = 0


Query: 54  MGSMKIVAVALIAILLVATLSSAAYAIDDDSMDLVFDGR---DINHGVPRKIMKWGVLNH 113
           M S KI+ V L+A++LVAT +      D D++DL+ DGR   DIN G             
Sbjct: 1   MESKKILPVVLVAMMLVATSTGFN---DGDTIDLISDGRAQIDINGG------------- 60

Query: 114 EERVCPKILMECKKDSDCLAECICLEHGYCG 142
              VCPKIL  C++DSDC   CICL +GYCG
Sbjct: 61  ---VCPKILQRCRRDSDCPGACICLGNGYCG 72

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BLAST of Carg19685 vs. TrEMBL
Match: tr|A0A0A7HIA9|A0A0A7HIA9_9ROSI (TIPRE5 (Fragment) OS=Momordica friesiorum OX=703365 GN=TIPRE5 PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 6.6e-08
Identity = 37/86 (43.02%), Postives = 53/86 (61.63%), Query Frame = 0


Query: 61  AVALIAILLVATLSSAAYAIDDDSMDLVFDGR-----DINHGVPRKIMKWGVLNHEERVC 120
           A AL+A++LVAT   +A +   D++ L+ +GR     DIN G        GV + E+R C
Sbjct: 1   AAALVAMMLVAT---SADSNGGDTIHLISNGRAQTGQDINSG--------GVYSEEQRAC 60

Query: 121 PKILMECKKDSDCLAECICLEHGYCG 142
           PKIL  C++DSDC   C+C ++GYCG
Sbjct: 61  PKILKRCRRDSDCPGACVCQDNGYCG 75

[Close]

BLAST of Carg19685 vs. TrEMBL
Match: tr|A0A0A7HG47|A0A0A7HG47_9ROSI (TIPRE1 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE1 PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.5e-07
Identity = 37/86 (43.02%), Postives = 52/86 (60.47%), Query Frame = 0


Query: 61  AVALIAILLVATLSSAAYAIDDDSMDLVFDGR-----DINHGVPRKIMKWGVLNHEERVC 120
           A AL+A++LVAT   +A     D++ L+ +GR     DIN G        GV + E+R C
Sbjct: 1   AAALVAMMLVAT---SADFNGGDTIHLISNGRAQTGQDINSG--------GVYSEEQRAC 60

Query: 121 PKILMECKKDSDCLAECICLEHGYCG 142
           PKIL  C++DSDC   C+C ++GYCG
Sbjct: 61  PKILKRCRRDSDCPGACVCQDNGYCG 75

[Close]

BLAST of Carg19685 vs. TrEMBL
Match: tr|A0A0A7HF33|A0A0A7HF33_9ROSI (TIPRE2 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE2 PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.5e-07
Identity = 37/86 (43.02%), Postives = 52/86 (60.47%), Query Frame = 0


Query: 61  AVALIAILLVATLSSAAYAIDDDSMDLVFDGR-----DINHGVPRKIMKWGVLNHEERVC 120
           A AL+A++LVAT   +A     D++ L+ +GR     DIN G        GV + E+R C
Sbjct: 1   AAALVAMMLVAT---SADFNGGDTIHLISNGRAQTGQDINSG--------GVYSEEQRAC 60

Query: 121 PKILMECKKDSDCLAECICLEHGYCG 142
           PKIL  C++DSDC   C+C ++GYCG
Sbjct: 61  PKILKRCRRDSDCPGACVCQDNGYCG 75

The following BLAST results are available for this feature:

BLAST of Carg19685 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022922566.1	4.3e-35	97.50	probable polygalacturonase At3g15720 [Cucurbita moschata]	[more]
XP_023552846.1	9.8e-32	94.87	probable polygalacturonase At3g15720 [Cucurbita pepo subsp. pepo]	[more]
KGN52764.1	8.4e-15	55.10	hypothetical protein Csa_4G000835 [Cucumis sativus]	[more]
XP_011654312.1	1.3e-12	61.76	PREDICTED: trypsin inhibitor 1-like [Cucumis sativus]	[more]
P10293.1	4.3e-11	100.00	RecName: Full=Trypsin inhibitor 3; AltName: Full=CPTI-III; AltName: Full=Trypsin...	[more]

BLAST of Carg19685 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of Carg19685 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|P10293\|ITR3_CUCPE	1.4e-13	100.00	Trypsin inhibitor 3 OS=Cucurbita pepo OX=3663 PE=1 SV=1	[more]
sp\|P07853\|ITR4_CUCMA	1.2e-12	90.63	Trypsin inhibitor 4 OS=Cucurbita maxima OX=3661 PE=1 SV=2	[more]
sp\|P01074\|ITR1_CUCMA	5.0e-11	93.10	Trypsin inhibitor 1 OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
sp\|P83398\|ITR7_CYCPE	2.1e-09	82.76	Trypsin inhibitor 7 OS=Cyclanthera pedata OX=198836 PE=1 SV=1	[more]
sp\|P83397\|ITR6_CYCPE	2.7e-09	82.76	Trypsin inhibitor 6 OS=Cyclanthera pedata OX=198836 PE=1 SV=1	[more]

BLAST of Carg19685 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KV23\|A0A0A0KV23_CUCSA	5.5e-15	55.10	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000835 PE=3 SV=1	[more]
tr\|J3R9Z5\|J3R9Z5_MOMCO	5.0e-08	45.05	Two inhibitor peptide topologies 3 OS=Momordica cochinchinensis OX=3674 GN=TIPTO...	[more]
tr\|A0A0A7HIA9\|A0A0A7HIA9_9ROSI	6.6e-08	43.02	TIPRE5 (Fragment) OS=Momordica friesiorum OX=703365 GN=TIPRE5 PE=3 SV=1	[more]
tr\|A0A0A7HG47\|A0A0A7HG47_9ROSI	1.5e-07	43.02	TIPRE1 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE1 PE=3 SV=1	[more]
tr\|A0A0A7HF33\|A0A0A7HF33_9ROSI	1.5e-07	43.02	TIPRE2 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE2 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004866	endopeptidase inhibitor activity
GO:0004867	serine-type endopeptidase inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR011052	Proteinase_amylase_inhib_sf
IPR000737	Prot_inh_squash

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg19685-RA	Carg19685-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000737	Proteinase inhibitor I7, squash	PRINTS	PR00293	SQUASHINHBTR	coord: 125..134 score: 80.5 coord: 115..124 score: 73.0
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 113..141 e-value: 1.8E-18 score: 66.1
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 115..134
IPR000737	Proteinase inhibitor I7, squash	CDD	cd00150	PlantTI	coord: 115..141 e-value: 4.57422E-15 score: 64.3211
None	No IPR available	SMART	SM00286	PTI_1	coord: 113..141 e-value: 5.7E-15 score: 65.7
None	No IPR available	GENE3D	G3DSA:3.10.450.30		coord: 103..141 e-value: 1.8E-11 score: 46.2
IPR011052	Proteinase/amylase inhibitor domain superfamily	SUPERFAMILY	SSF57027	Plant inhibitors of proteinases and amylases	coord: 110..141

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg19685	Cla006424	Watermelon (97103) v1	carwmB0287
Carg19685	Csa4G000810	Cucumber (Chinese Long) v2	carcuB0322
Carg19685	MELO3C018005	Melon (DHL92) v3.5.1	carmeB0297
Carg19685	ClCG10G010420	Watermelon (Charleston Gray)	carwcgB0287
Carg19685	CSPI04G00300	Wild cucumber (PI 183967)	carcpiB0328
Carg19685	Cucsa.097530	Cucumber (Gy14) v1	carcgyB0343
Carg19685	CsGy4G000280	Cucumber (Gy14) v2	carcgybB0309
Carg19685	MELO3C018005.2	Melon (DHL92) v3.6.1	carmedB0308
Carg19685	CsaV3_4G000290	Cucumber (Chinese Long) v3	carcucB0328
Carg19685	Cla97C10G193330	Watermelon (97103) v2	carwmbB0302
Carg19685	Bhi11G000948	Wax gourd	carwgoB0381

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg19685	Silver-seed gourd	carcarB324
Carg19685	Cucumber (Gy14) v1	carcgyB0344
Carg19685	Cucumber (Gy14) v1	carcgyB0348
Carg19685	Cucurbita maxima (Rimu)	carcmaB0405
Carg19685	Cucurbita maxima (Rimu)	carcmaB0406
Carg19685	Cucurbita maxima (Rimu)	carcmaB0407
Carg19685	Cucurbita maxima (Rimu)	carcmaB0408
Carg19685	Cucurbita moschata (Rifu)	carcmoB0401
Carg19685	Cucurbita moschata (Rifu)	carcmoB0402
Carg19685	Cucurbita moschata (Rifu)	carcmoB0403
Carg19685	Cucurbita pepo (Zucchini)	carcpeB0407
Carg19685	Cucurbita pepo (Zucchini)	carcpeB0408
Carg19685	Cucurbita pepo (Zucchini)	carcpeB0409
Carg19685	Wild cucumber (PI 183967)	carcpiB0329
Carg19685	Cucumber (Chinese Long) v2	carcuB0323
Carg19685	Cucumber (Chinese Long) v3	carcucB0329
Carg19685	Bottle gourd (USVL1VR-Ls)	carlsiB269
Carg19685	Melon (DHL92) v3.5.1	carmeB0295
Carg19685	Melon (DHL92) v3.6.1	carmedB0306
Carg19685	Watermelon (Charleston Gray)	carwcgB0288
Carg19685	Watermelon (97103) v2	carwmbB0303
Carg19685	Watermelon (97103) v2	carwmbB0305
Carg19685	Wax gourd	carwgoB0383