Cp4.1LG14g04630 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG14g04630
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	Trypsin inhibitor 1
Location	Cp4.1LG14 : 1540040 .. 1540279 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTGGAAAAGAGGTGCTTTAATTGCCATGGTGGGGCTGTTGCTGATGGCGGCTTTTGCAGACTCCGCCGCCGTCCATGGCGGCGAAGTGATTCAGCTAGTTTCCGACGGAGTGAATGATTTCCCAAGGAAGATGATGAACGCTGCCCTTGGTTGCCCTAGAATCCTTATGGAGTGCGAAGTGGATTCCGACTGCCTTCCTGGTTGCGTATGCCGGCCCAACGGCTTCTGTGGTTGA

mRNA sequence

ATGGAGTGGAAAAGAGGTGCTTTAATTGCCATGGTGGGGCTGTTGCTGATGGCGGCTTTTGCAGACTCCGCCGCCGTCCATGGCGGCGAAGTGATTCAGCTAGTTTCCGACGGAGTGAATGATTTCCCAAGGAAGATGATGAACGCTGCCCTTGGTTGCCCTAGAATCCTTATGGAGTGCGAAGTGGATTCCGACTGCCTTCCTGGTTGCGTATGCCGGCCCAACGGCTTCTGTGGTTGA

Coding sequence (CDS)

ATGGAGTGGAAAAGAGGTGCTTTAATTGCCATGGTGGGGCTGTTGCTGATGGCGGCTTTTGCAGACTCCGCCGCCGTCCATGGCGGCGAAGTGATTCAGCTAGTTTCCGACGGAGTGAATGATTTCCCAAGGAAGATGATGAACGCTGCCCTTGGTTGCCCTAGAATCCTTATGGAGTGCGAAGTGGATTCCGACTGCCTTCCTGGTTGCGTATGCCGGCCCAACGGCTTCTGTGGTTGA

Protein sequence

MEWKRGALIAMVGLLLMAAFADSAAVHGGEVIQLVSDGVNDFPRKMMNAALGCPRILMECEVDSDCLPGCVCRPNGFCG

Homology

[Close]

BLAST of Cp4.1LG14g04630 vs. Swiss-Prot
Match: ITR1_TRIKI (Trypsin inhibitor 1 OS=Trichosanthes kirilowii PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 8.3e-16
Identity = 39/65 (60.00%), Postives = 46/65 (70.77%), Query Frame = 1


Query: 17 MAAFADSAAVHGG--EVIQLVSDGVNDFPRKMMNAALGCPRILMECEVDSDCLPGCVCRP 76
          MAAF +SA    G  EVIQLVSDGVN++  KMM   + CPRILM C+V+ DCL GC C  
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMEGVVACPRILMPCKVNDDCLRGCKCLS 60

Query: 77 NGFCG 80
          NG+CG
Sbjct: 61 NGYCG 65

[Close]

BLAST of Cp4.1LG14g04630 vs. Swiss-Prot
Match: ITR5_LUFAE (Trypsin inhibitor 5 OS=Luffa aegyptiaca PE=1 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 5.6e-12
Identity = 35/65 (53.85%), Postives = 45/65 (69.23%), Query Frame = 1


Query: 17 MAAFADSAAVHGGEVIQLVSDGVNDFPRKMMNAALG--CPRILMECEVDSDCLPGCVCRP 76
          MA+ A+S+ V   EVI+L+SDG ND PRK+M+   G  CPRILM C+ D DC+  C C  
Sbjct: 1  MASVAESSGVV--EVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLS 60

Query: 77 NGFCG 80
          NG+CG
Sbjct: 61 NGYCG 63

[Close]

BLAST of Cp4.1LG14g04630 vs. Swiss-Prot
Match: ITR2_ECBEL (Trypsin inhibitor 2 OS=Ecballium elaterium PE=1 SV=2)

HSP 1 Score: 59.3 bits (142), Expect = 2.2e-08
Identity = 23/28 (82.14%), Postives = 24/28 (85.71%), Query Frame = 1


Query: 52 GCPRILMECEVDSDCLPGCVCRPNGFCG 80
          GCPRILM C+ DSDCL GCVC PNGFCG
Sbjct: 1  GCPRILMRCKQDSDCLAGCVCGPNGFCG 28

[Close]

BLAST of Cp4.1LG14g04630 vs. Swiss-Prot
Match: ITR2_BRYDI (Trypsin inhibitor 2 OS=Bryonia dioica PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 2.4e-07
Identity = 21/28 (75.00%), Postives = 24/28 (85.71%), Query Frame = 1


Query: 52 GCPRILMECEVDSDCLPGCVCRPNGFCG 80
          GCPRILM C+ DSDCL GCVC+ NG+CG
Sbjct: 2  GCPRILMRCKRDSDCLAGCVCQKNGYCG 29

[Close]

BLAST of Cp4.1LG14g04630 vs. Swiss-Prot
Match: ITR5_SECED (Trypsin inhibitor 5 OS=Sechium edule PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 4.1e-07
Identity = 19/27 (70.37%), Postives = 24/27 (88.89%), Query Frame = 1


Query: 53 CPRILMECEVDSDCLPGCVCRPNGFCG 80
          CPRILM+C++D+DC P C CRP+GFCG
Sbjct: 1  CPRILMKCKLDTDCFPTCTCRPSGFCG 27

[Close]

BLAST of Cp4.1LG14g04630 vs. TrEMBL
Match: A0A0A0KY34_CUCSA (Trypsin inhibitor 1 OS=Cucumis sativus GN=Csa_4G000810 PE=3 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 1.2e-18
Identity = 46/81 (56.79%), Postives = 62/81 (76.54%), Query Frame = 1


Query: 1  MEWKRGALIAMVGLLLMAAFADSAAVH-GGEVIQLVSDGVNDFPRKM-MNAALGCPRILM 60
          MEWK+ AL+AM+G+LLMA F +S  +    E+IQLVSDGVN++ + +  + A GCPRILM
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60

Query: 61 ECEVDSDCLPGCVCRPNGFCG 80
          +C+ DSDC PGC C+P G+CG
Sbjct: 61 KCKTDSDCYPGCTCKPMGYCG 81

[Close]

BLAST of Cp4.1LG14g04630 vs. TrEMBL
Match: A0A0A0KT01_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G000805 PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 7.3e-11
Identity = 39/82 (47.56%), Postives = 50/82 (60.98%), Query Frame = 1


Query: 1  MEWKRGALIAMVGLLLMAAFADS--AAVHGGEVIQLVSDGVNDFPRKMMNAALG-CPRIL 60
          MEWK+  ++A+VG+L+MAA  +   AA+    V  L     N+ PRKMMN     CPRIL
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGL---NKNELPRKMMNEETRMCPRIL 60

Query: 61 MECEVDSDCLPGCVCRPNGFCG 80
           EC  D DC+  C+C  NGFCG
Sbjct: 61 EECTTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of Cp4.1LG14g04630 vs. TrEMBL
Match: A0A0A0KV23_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G000835 PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 3.2e-06
Identity = 39/98 (39.80%), Postives = 54/98 (55.10%), Query Frame = 1


Query: 1  MEWKRGALIAMVGLLLMAAFADSAA---VHGGEVIQLVSDGV--------NDFPRKMM-- 60
          ME K+  ++A+V  +++     S+A   V   + I+LVSDG         N  PRKMM  
Sbjct: 1  MESKKIVMVAIVATIIVQVAMSSSASFVVDDKDWIKLVSDGRAQADQDMNNGCPRKMMIK 60

Query: 61 -----NAALGCPRILMECEVDSDCLPGCVCRPN-GFCG 80
                  + CPRILM+C+ DSDCLPGCVC  +  +CG
Sbjct: 61 GGVFKEEEMMCPRILMKCKHDSDCLPGCVCLEHIEYCG 98

[Close]

BLAST of Cp4.1LG14g04630 vs. TrEMBL
Match: A0A0A7HF87_9ROSI (TIPTOP4 protein OS=Momordica subangulata GN=TIPTOP4 PE=3 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 5.4e-06
Identity = 32/80 (40.00%), Postives = 45/80 (56.25%), Query Frame = 1


Query: 1  MEWKRGALIAMVGLLLMAAFADSAAVHGGEVIQLVSDGVNDFPRKMMNAALG-CPRILME 60
          ME K+  L+ +V ++L+A    S   + G+   L+SDG     R  M+   G CP+IL  
Sbjct: 1  MESKKILLVVLVAMMLVAT---STCFNDGDTTDLISDG-----RAQMDINGGACPKILQR 60

Query: 61 CEVDSDCLPGCVCRPNGFCG 80
          C  DSDC   C+CR NG+CG
Sbjct: 61 CRRDSDCPSACICRGNGYCG 72

[Close]

BLAST of Cp4.1LG14g04630 vs. NCBI nr
Match: gi|700197603|gb|KGN52761.1| (Trypsin inhibitor 1 [Cucumis sativus])

HSP 1 Score: 100.1 bits (248), Expect = 1.8e-18
Identity = 46/81 (56.79%), Postives = 62/81 (76.54%), Query Frame = 1


Query: 1  MEWKRGALIAMVGLLLMAAFADSAAVH-GGEVIQLVSDGVNDFPRKM-MNAALGCPRILM 60
          MEWK+ AL+AM+G+LLMA F +S  +    E+IQLVSDGVN++ + +  + A GCPRILM
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60

Query: 61 ECEVDSDCLPGCVCRPNGFCG 80
          +C+ DSDC PGC C+P G+CG
Sbjct: 61 KCKTDSDCYPGCTCKPMGYCG 81

[Close]

BLAST of Cp4.1LG14g04630 vs. NCBI nr
Match: gi|8134511|sp|Q43667.1|ITR1_TRIKI (RecName: Full=Trypsin inhibitor 1; AltName: Full=TTII; AltName: Full=Trypsin inhibitor I; Flags: Precursor)

HSP 1 Score: 84.0 bits (206), Expect = 1.3e-13
Identity = 39/65 (60.00%), Postives = 46/65 (70.77%), Query Frame = 1


Query: 17 MAAFADSAAVHGG--EVIQLVSDGVNDFPRKMMNAALGCPRILMECEVDSDCLPGCVCRP 76
          MAAF +SA    G  EVIQLVSDGVN++  KMM   + CPRILM C+V+ DCL GC C  
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMEGVVACPRILMPCKVNDDCLRGCKCLS 60

Query: 77 NGFCG 80
          NG+CG
Sbjct: 61 NGYCG 65

[Close]

BLAST of Cp4.1LG14g04630 vs. NCBI nr
Match: gi|778697386|ref|XP_011654311.1| (PREDICTED: uncharacterized protein LOC105435333 [Cucumis sativus])

HSP 1 Score: 74.3 bits (181), Expect = 1.0e-10
Identity = 39/82 (47.56%), Postives = 50/82 (60.98%), Query Frame = 1


Query: 1   MEWKRGALIAMVGLLLMAAFADS--AAVHGGEVIQLVSDGVNDFPRKMMNAALG-CPRIL 60
           MEWK+  ++A+VG+L+MAA  +   AA+    V  L     N+ PRKMMN     CPRIL
Sbjct: 126 MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGL---NKNELPRKMMNEETRMCPRIL 185

Query: 61  MECEVDSDCLPGCVCRPNGFCG 80
            EC  D DC+  C+C  NGFCG
Sbjct: 186 EECTTDDDCMNDCICLSNGFCG 204

[Close]

BLAST of Cp4.1LG14g04630 vs. NCBI nr
Match: gi|700197602|gb|KGN52760.1| (hypothetical protein Csa_4G000805 [Cucumis sativus])

HSP 1 Score: 74.3 bits (181), Expect = 1.0e-10
Identity = 39/82 (47.56%), Postives = 50/82 (60.98%), Query Frame = 1


Query: 1  MEWKRGALIAMVGLLLMAAFADS--AAVHGGEVIQLVSDGVNDFPRKMMNAALG-CPRIL 60
          MEWK+  ++A+VG+L+MAA  +   AA+    V  L     N+ PRKMMN     CPRIL
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGL---NKNELPRKMMNEETRMCPRIL 60

Query: 61 MECEVDSDCLPGCVCRPNGFCG 80
           EC  D DC+  C+C  NGFCG
Sbjct: 61 EECTTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of Cp4.1LG14g04630 vs. NCBI nr
Match: gi|462426|sp|P34950.1|ITR5_LUFAE (RecName: Full=Trypsin inhibitor 5; AltName: Full=TGT-II; AltName: Full=Trypsin inhibitor II; Flags: Precursor)

HSP 1 Score: 71.2 bits (173), Expect = 8.9e-10
Identity = 35/65 (53.85%), Postives = 45/65 (69.23%), Query Frame = 1


Query: 17 MAAFADSAAVHGGEVIQLVSDGVNDFPRKMMNAALG--CPRILMECEVDSDCLPGCVCRP 76
          MA+ A+S+ V   EVI+L+SDG ND PRK+M+   G  CPRILM C+ D DC+  C C  
Sbjct: 1  MASVAESSGVV--EVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLS 60

Query: 77 NGFCG 80
          NG+CG
Sbjct: 61 NGYCG 63

The following BLAST results are available for this feature:

BLAST of Cp4.1LG14g04630 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ITR1_TRIKI	8.3e-16	60.00	Trypsin inhibitor 1 OS=Trichosanthes kirilowii PE=3 SV=1	[more]
ITR5_LUFAE	5.6e-12	53.85	Trypsin inhibitor 5 OS=Luffa aegyptiaca PE=1 SV=1	[more]
ITR2_ECBEL	2.2e-08	82.14	Trypsin inhibitor 2 OS=Ecballium elaterium PE=1 SV=2	[more]
ITR2_BRYDI	2.4e-07	75.00	Trypsin inhibitor 2 OS=Bryonia dioica PE=1 SV=1	[more]
ITR5_SECED	4.1e-07	70.37	Trypsin inhibitor 5 OS=Sechium edule PE=1 SV=1	[more]

BLAST of Cp4.1LG14g04630 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KY34_CUCSA	1.2e-18	56.79	Trypsin inhibitor 1 OS=Cucumis sativus GN=Csa_4G000810 PE=3 SV=1	[more]
A0A0A0KT01_CUCSA	7.3e-11	47.56	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G000805 PE=3 SV=1	[more]
A0A0A0KV23_CUCSA	3.2e-06	39.80	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G000835 PE=3 SV=1	[more]
A0A0A7HF87_9ROSI	5.4e-06	40.00	TIPTOP4 protein OS=Momordica subangulata GN=TIPTOP4 PE=3 SV=1	[more]

BLAST of Cp4.1LG14g04630 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of Cp4.1LG14g04630 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700197603\|gb\|KGN52761.1\|	1.8e-18	56.79	Trypsin inhibitor 1 [Cucumis sativus]	[more]
gi\|8134511\|sp\|Q43667.1\|ITR1_TRIKI	1.3e-13	60.00	RecName: Full=Trypsin inhibitor 1; AltName: Full=TTII; AltName: Full=Trypsin inh...	[more]
gi\|778697386\|ref\|XP_011654311.1\|	1.0e-10	47.56	PREDICTED: uncharacterized protein LOC105435333 [Cucumis sativus]	[more]
gi\|700197602\|gb\|KGN52760.1\|	1.0e-10	47.56	hypothetical protein Csa_4G000805 [Cucumis sativus]	[more]
gi\|462426\|sp\|P34950.1\|ITR5_LUFAE	8.9e-10	53.85	RecName: Full=Trypsin inhibitor 5; AltName: Full=TGT-II; AltName: Full=Trypsin i...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004866	endopeptidase inhibitor activity
GO:0004867	serine-type endopeptidase inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR011052	Proteinase_amylase_inhib_sf
IPR000737	Prot_inh_squash

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG14g04630.1	Cp4.1LG14g04630.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 53..79 score: 7.1
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 53..72 scor
IPR011052	Proteinase/amylase inhibitor domain	unknown	SSF57027	Plant inhibitors of proteinases and amylases	coord: 52..79 score: 7.38
None	No IPR available	SMART	SM00286	PTI_1	coord: 51..79 score: 4.

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cp4.1LG14g04630	Cucsa.097530	Cucumber (Gy14) v1	cgycpeB0188
Cp4.1LG14g04630	Cla006431	Watermelon (97103) v1	cpewmB204
Cp4.1LG14g04630	Csa4G000810	Cucumber (Chinese Long) v2	cpecuB219
Cp4.1LG14g04630	MELO3C018005	Melon (DHL92) v3.5.1	cpemeB201
Cp4.1LG14g04630	ClCG10G010350	Watermelon (Charleston Gray)	cpewcgB190
Cp4.1LG14g04630	CSPI04G00330	Wild cucumber (PI 183967)	cpecpiB221
Cp4.1LG14g04630	CsaV3_4G000290	Cucumber (Chinese Long) v3	cpecucB0263

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG14g04630	Bottle gourd (USVL1VR-Ls)	cpelsiB168
Cp4.1LG14g04630	Melon (DHL92) v3.5.1	cpemeB200
Cp4.1LG14g04630	Melon (DHL92) v3.5.1	cpemeB182
Cp4.1LG14g04630	Melon (DHL92) v3.5.1	cpemeB208
Cp4.1LG14g04630	Cucumber (Gy14) v2	cgybcpeB485
Cp4.1LG14g04630	Cucumber (Gy14) v2	cgybcpeB492
Cp4.1LG14g04630	Cucumber (Gy14) v2	cgybcpeB747
Cp4.1LG14g04630	Melon (DHL92) v3.6.1	cpemedB220
Cp4.1LG14g04630	Melon (DHL92) v3.6.1	cpemedB243
Cp4.1LG14g04630	Melon (DHL92) v3.6.1	cpemedB242
Cp4.1LG14g04630	Melon (DHL92) v3.6.1	cpemedB250
Cp4.1LG14g04630	Silver-seed gourd	carcpeB0407
Cp4.1LG14g04630	Silver-seed gourd	carcpeB0523
Cp4.1LG14g04630	Cucumber (Chinese Long) v3	cpecucB0245
Cp4.1LG14g04630	Cucumber (Chinese Long) v3	cpecucB0269
Cp4.1LG14g04630	Cucumber (Chinese Long) v3	cpecucB0283
Cp4.1LG14g04630	Wax gourd	cpewgoB0266
Cp4.1LG14g04630	Wax gourd	cpewgoB0304
Cp4.1LG14g04630	Cucurbita pepo (Zucchini)	cpecpeB234
Cp4.1LG14g04630	Cucumber (Gy14) v1	cgycpeB0215
Cp4.1LG14g04630	Cucurbita maxima (Rimu)	cmacpeB114
Cp4.1LG14g04630	Cucurbita maxima (Rimu)	cmacpeB344
Cp4.1LG14g04630	Cucurbita maxima (Rimu)	cmacpeB715
Cp4.1LG14g04630	Cucurbita moschata (Rifu)	cmocpeB092
Cp4.1LG14g04630	Cucurbita moschata (Rifu)	cmocpeB307
Cp4.1LG14g04630	Cucurbita moschata (Rifu)	cmocpeB668
Cp4.1LG14g04630	Cucumber (Chinese Long) v2	cpecuB229