CmaCh16G003170 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh16G003170
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Trypsin inhibitor 1
Location	Cma_Chr16 : 1504018 .. 1504269 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGTGGAAAAGAGGTGCTTTGGTTGCAATGGTGGGGATCTTGCTGGTGGCTGCTTTTGCAGAGTCCGCCGCCGTCGACGCCGCCGTCGATGAAGTGAGTCAATTAGTTTCCGTTGGAGGGAATGGTTTTCATTCCCCAAGAAAGATGATGAACGTTGTCCAAGGGTGCCCTACAATCCTTATGTCCTGCAAAGTGGATTCCGACTGCCTTCCTGCTTGCAAATGCCGGTCCAACGGCTACTGTAGTTGA

mRNA sequence

ATGGGGTGGAAAAGAGGTGCTTTGGTTGCAATGGTGGGGATCTTGCTGGTGGCTGCTTTTGCAGAGTCCGCCGCCGTCGACGCCGCCGTCGATGAAGTGAGTCAATTAGTTTCCGTTGGAGGGAATGGTTTTCATTCCCCAAGAAAGATGATGAACGTTGTCCAAGGGTGCCCTACAATCCTTATGTCCTGCAAAGTGGATTCCGACTGCCTTCCTGCTTGCAAATGCCGGTCCAACGGCTACTGTAGTTGA

Coding sequence (CDS)

ATGGGGTGGAAAAGAGGTGCTTTGGTTGCAATGGTGGGGATCTTGCTGGTGGCTGCTTTTGCAGAGTCCGCCGCCGTCGACGCCGCCGTCGATGAAGTGAGTCAATTAGTTTCCGTTGGAGGGAATGGTTTTCATTCCCCAAGAAAGATGATGAACGTTGTCCAAGGGTGCCCTACAATCCTTATGTCCTGCAAAGTGGATTCCGACTGCCTTCCTGCTTGCAAATGCCGGTCCAACGGCTACTGTAGTTGA

Protein sequence

MGWKRGALVAMVGILLVAAFAESAAVDAAVDEVSQLVSVGGNGFHSPRKMMNVVQGCPTILMSCKVDSDCLPACKCRSNGYCS

Homology

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BLAST of CmaCh16G003170 vs. Swiss-Prot
Match: ITR1_TRIKI (Trypsin inhibitor 1 OS=Trichosanthes kirilowii PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 4.5e-12
Identity = 39/66 (59.09%), Postives = 42/66 (63.64%), Query Frame = 1


Query: 17 VAAFAESAAVDAAVDEVSQLVSVGGNGFHSPRKMMNVVQGCPTILMSCKVDSDCLPACKC 76
          +AAF ESA   A  DEV QLVS G N +    KMM  V  CP ILM CKV+ DCL  CKC
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSE--KMMEGVVACPRILMPCKVNDDCLRGCKC 60

Query: 77 RSNGYC 83
           SNGYC
Sbjct: 61 LSNGYC 64

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BLAST of CmaCh16G003170 vs. Swiss-Prot
Match: ITR5_LUFAE (Trypsin inhibitor 5 OS=Luffa aegyptiaca PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.8e-08
Identity = 32/62 (51.61%), Postives = 39/62 (62.90%), Query Frame = 1


Query: 23 SAAVDAAVDEVSQLVSVGGNGFHSPRKMMNVVQG--CPTILMSCKVDSDCLPACKCRSNG 82
          S A  + V EV +L+S GGN    PRK+M+   G  CP ILM CK D DC+  C+C SNG
Sbjct: 3  SVAESSGVVEVIELISDGGNDL--PRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLSNG 62

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BLAST of CmaCh16G003170 vs. TrEMBL
Match: A0A0A0KY34_CUCSA (Trypsin inhibitor 1 OS=Cucumis sativus GN=Csa_4G000810 PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 1.0e-10
Identity = 41/82 (50.00%), Postives = 51/82 (62.20%), Query Frame = 1


Query: 1  MGWKRGALVAMVGILLVAAFAESAAVDAAVDEVSQLVSVGGNGFHSPRKMMNVVQGCPTI 60
          M WK+ ALVAM+G+LL+A F ES  +    +E+ QLVS G N + S     +   GCP I
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVD-EEIIQLVSDGVNEY-SQNIGKDTAPGCPRI 60

Query: 61 LMSCKVDSDCLPACKCRSNGYC 83
          LM CK DSDC P C C+  GYC
Sbjct: 61 LMKCKTDSDCYPGCTCKPMGYC 80

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BLAST of CmaCh16G003170 vs. NCBI nr
Match: gi|700197603|gb|KGN52761.1| (Trypsin inhibitor 1 [Cucumis sativus])

HSP 1 Score: 73.9 bits (180), Expect = 1.4e-10
Identity = 41/82 (50.00%), Postives = 51/82 (62.20%), Query Frame = 1


Query: 1  MGWKRGALVAMVGILLVAAFAESAAVDAAVDEVSQLVSVGGNGFHSPRKMMNVVQGCPTI 60
          M WK+ ALVAM+G+LL+A F ES  +    +E+ QLVS G N + S     +   GCP I
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVD-EEIIQLVSDGVNEY-SQNIGKDTAPGCPRI 60

Query: 61 LMSCKVDSDCLPACKCRSNGYC 83
          LM CK DSDC P C C+  GYC
Sbjct: 61 LMKCKTDSDCYPGCTCKPMGYC 80

[Close]

BLAST of CmaCh16G003170 vs. NCBI nr
Match: gi|8134511|sp|Q43667.1|ITR1_TRIKI (RecName: Full=Trypsin inhibitor 1; AltName: Full=TTII; AltName: Full=Trypsin inhibitor I; Flags: Precursor)

HSP 1 Score: 71.6 bits (174), Expect = 7.1e-10
Identity = 39/66 (59.09%), Postives = 42/66 (63.64%), Query Frame = 1


Query: 17 VAAFAESAAVDAAVDEVSQLVSVGGNGFHSPRKMMNVVQGCPTILMSCKVDSDCLPACKC 76
          +AAF ESA   A  DEV QLVS G N +    KMM  V  CP ILM CKV+ DCL  CKC
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSE--KMMEGVVACPRILMPCKVNDDCLRGCKC 60

Query: 77 RSNGYC 83
           SNGYC
Sbjct: 61 LSNGYC 64

[Close]

BLAST of CmaCh16G003170 vs. NCBI nr
Match: gi|462426|sp|P34950.1|ITR5_LUFAE (RecName: Full=Trypsin inhibitor 5; AltName: Full=TGT-II; AltName: Full=Trypsin inhibitor II; Flags: Precursor)

HSP 1 Score: 59.7 bits (143), Expect = 2.8e-06
Identity = 32/62 (51.61%), Postives = 39/62 (62.90%), Query Frame = 1


Query: 23 SAAVDAAVDEVSQLVSVGGNGFHSPRKMMNVVQG--CPTILMSCKVDSDCLPACKCRSNG 82
          S A  + V EV +L+S GGN    PRK+M+   G  CP ILM CK D DC+  C+C SNG
Sbjct: 3  SVAESSGVVEVIELISDGGNDL--PRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLSNG 62

The following BLAST results are available for this feature:

BLAST of CmaCh16G003170 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ITR1_TRIKI	4.5e-12	59.09	Trypsin inhibitor 1 OS=Trichosanthes kirilowii PE=3 SV=1	[more]
ITR5_LUFAE	1.8e-08	51.61	Trypsin inhibitor 5 OS=Luffa aegyptiaca PE=1 SV=1	[more]

BLAST of CmaCh16G003170 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KY34_CUCSA	1.0e-10	50.00	Trypsin inhibitor 1 OS=Cucumis sativus GN=Csa_4G000810 PE=3 SV=1	[more]

BLAST of CmaCh16G003170 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of CmaCh16G003170 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700197603\|gb\|KGN52761.1\|	1.4e-10	50.00	Trypsin inhibitor 1 [Cucumis sativus]	[more]
gi\|8134511\|sp\|Q43667.1\|ITR1_TRIKI	7.1e-10	59.09	RecName: Full=Trypsin inhibitor 1; AltName: Full=TTII; AltName: Full=Trypsin inh...	[more]
gi\|462426\|sp\|P34950.1\|ITR5_LUFAE	2.8e-06	51.61	RecName: Full=Trypsin inhibitor 5; AltName: Full=TGT-II; AltName: Full=Trypsin i...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000737	Prot_inh_squash
IPR011052	Proteinase_amylase_inhib_sf

Vocabulary: **Molecular Function**
Term	Definition
GO:0004867	serine-type endopeptidase inhibitor activity
GO:0004866	endopeptidase inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G003170.1	CmaCh16G003170.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 56..82 score: 2.0
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 57..76 scor
IPR011052	Proteinase/amylase inhibitor domain	unknown	SSF57027	Plant inhibitors of proteinases and amylases	coord: 55..82 score: 1.88
None	No IPR available	SMART	SM00286	PTI_1	coord: 55..83 score: 6.

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh16G003170	Cla006431	Watermelon (97103) v1	cmawmB293
CmaCh16G003170	ClCG10G010350	Watermelon (Charleston Gray)	cmawcgB280
CmaCh16G003170	CmoCh16G003360	Cucurbita moschata (Rifu)	cmacmoB322
CmaCh16G003170	Cp4.1LG14g04640	Cucurbita pepo (Zucchini)	cmacpeB344
CmaCh16G003170	CsaV3_4G000290	Cucumber (Chinese Long) v3	cmacucB0388
CmaCh16G003170	Bhi11G000936	Wax gourd	cmawgoB0410
CmaCh16G003170	CsGy4G000290	Cucumber (Gy14) v2	cgybcmaB494

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh16G003170	Cucumber (Gy14) v2	cgybcmaB750
CmaCh16G003170	Melon (DHL92) v3.6.1	cmamedB331
CmaCh16G003170	Melon (DHL92) v3.6.1	cmamedB354
CmaCh16G003170	Melon (DHL92) v3.6.1	cmamedB363
CmaCh16G003170	Silver-seed gourd	carcmaB0406
CmaCh16G003170	Silver-seed gourd	carcmaB0526
CmaCh16G003170	Cucumber (Chinese Long) v3	cmacucB0413
CmaCh16G003170	Cucumber (Chinese Long) v3	cmacucB0414
CmaCh16G003170	Watermelon (97103) v2	cmawmbB332
CmaCh16G003170	Watermelon (97103) v2	cmawmbB342
CmaCh16G003170	Wax gourd	cmawgoB0446
CmaCh16G003170	Cucurbita maxima (Rimu)	cmacmaB135
CmaCh16G003170	Cucurbita maxima (Rimu)	cmacmaB340
CmaCh16G003170	Cucurbita maxima (Rimu)	cmacmaB351
CmaCh16G003170	Cucumber (Gy14) v1	cgycmaB0226
CmaCh16G003170	Cucumber (Gy14) v1	cgycmaB0623
CmaCh16G003170	Cucurbita moschata (Rifu)	cmacmoB318
CmaCh16G003170	Cucurbita moschata (Rifu)	cmacmoB338
CmaCh16G003170	Wild cucumber (PI 183967)	cmacpiB338
CmaCh16G003170	Wild cucumber (PI 183967)	cmacpiB351
CmaCh16G003170	Cucumber (Chinese Long) v2	cmacuB333
CmaCh16G003170	Cucumber (Chinese Long) v2	cmacuB347
CmaCh16G003170	Cucumber (Chinese Long) v2	cmacuB348
CmaCh16G003170	Melon (DHL92) v3.5.1	cmameB287
CmaCh16G003170	Melon (DHL92) v3.5.1	cmameB303
CmaCh16G003170	Melon (DHL92) v3.5.1	cmameB311
CmaCh16G003170	Watermelon (Charleston Gray)	cmawcgB293
CmaCh16G003170	Cucurbita pepo (Zucchini)	cmacpeB351
CmaCh16G003170	Bottle gourd (USVL1VR-Ls)	cmalsiB304
CmaCh16G003170	Bottle gourd (USVL1VR-Ls)	cmalsiB307