Cp4.1LG14g04640 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG14g04640
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	Trypsin inhibitor 1
Location	Cp4.1LG14 : 1535675 .. 1535908 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGCGGAAAAGAGGTGCTTTAGTTGCAATGGTGGGGCTGTTGCTGATAGCCGCTTTTGCAGAGTCCACCGCCGTGGATGAAGTGATTCAACTAGTTTCCGATGGAGGGAATGATTTCCCAAGAAAGATGATGAAGGGCGCCGAAGAGTGTCCTAGACAGCTTGTCAGGTGCAAGAAGGATTCCGACTGCCATCGTGCTTGCAAATGCATGCCGTACCACTTCTGTGGTTAA

mRNA sequence

ATGGAGCGGAAAAGAGGTGCTTTAGTTGCAATGGTGGGGCTGTTGCTGATAGCCGCTTTTGCAGAGTCCACCGCCGTGGATGAAGTGATTCAACTAGTTTCCGATGGAGGGAATGATTTCCCAAGAAAGATGATGAAGGGCGCCGAAGAGTGTCCTAGACAGCTTGTCAGGTGCAAGAAGGATTCCGACTGCCATCGTGCTTGCAAATGCATGCCGTACCACTTCTGTGGTTAA

Coding sequence (CDS)

ATGGAGCGGAAAAGAGGTGCTTTAGTTGCAATGGTGGGGCTGTTGCTGATAGCCGCTTTTGCAGAGTCCACCGCCGTGGATGAAGTGATTCAACTAGTTTCCGATGGAGGGAATGATTTCCCAAGAAAGATGATGAAGGGCGCCGAAGAGTGTCCTAGACAGCTTGTCAGGTGCAAGAAGGATTCCGACTGCCATCGTGCTTGCAAATGCATGCCGTACCACTTCTGTGGTTAA

Protein sequence

MERKRGALVAMVGLLLIAAFAESTAVDEVIQLVSDGGNDFPRKMMKGAEECPRQLVRCKKDSDCHRACKCMPYHFCG

Homology

[Close]

BLAST of Cp4.1LG14g04640 vs. Swiss-Prot
Match: ITR5_LUFAE (Trypsin inhibitor 5 OS=Luffa aegyptiaca PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.2e-11
Identity = 33/63 (52.38%), Postives = 43/63 (68.25%), Query Frame = 1


Query: 17 IAAFAESTAVDEVIQLVSDGGNDFPRKMMKGAEE--CPRQLVRCKKDSDCHRACKCMPYH 76
          +A+ AES+ V EVI+L+SDGGND PRK+M G     CPR L+ CK D DC   C+C+   
Sbjct: 1  MASVAESSGVVEVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLSNG 60

Query: 77 FCG 78
          +CG
Sbjct: 61 YCG 63

[Close]

BLAST of Cp4.1LG14g04640 vs. Swiss-Prot
Match: ITR1_TRIKI (Trypsin inhibitor 1 OS=Trichosanthes kirilowii PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 1.6e-11
Identity = 34/65 (52.31%), Postives = 42/65 (64.62%), Query Frame = 1


Query: 17 IAAFAEST----AVDEVIQLVSDGGNDFPRKMMKGAEECPRQLVRCKKDSDCHRACKCMP 76
          +AAF ES       DEVIQLVSDG N++  KMM+G   CPR L+ CK + DC R CKC+ 
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMEGVVACPRILMPCKVNDDCLRGCKCLS 60

Query: 77 YHFCG 78
            +CG
Sbjct: 61 NGYCG 65

[Close]

BLAST of Cp4.1LG14g04640 vs. TrEMBL
Match: A0A0A0KY34_CUCSA (Trypsin inhibitor 1 OS=Cucumis sativus GN=Csa_4G000810 PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 4.9e-12
Identity = 41/81 (50.62%), Postives = 56/81 (69.14%), Query Frame = 1


Query: 1  MERKRGALVAMVGLLLIAAFAESTAV---DEVIQLVSDGGNDFPRKMMKG-AEECPRQLV 60
          ME K+ ALVAM+G+LL+A F ES  +   +E+IQLVSDG N++ + + K  A  CPR L+
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60

Query: 61 RCKKDSDCHRACKCMPYHFCG 78
          +CK DSDC+  C C P  +CG
Sbjct: 61 KCKTDSDCYPGCTCKPMGYCG 81

[Close]

BLAST of Cp4.1LG14g04640 vs. NCBI nr
Match: gi|700197603|gb|KGN52761.1| (Trypsin inhibitor 1 [Cucumis sativus])

HSP 1 Score: 78.2 bits (191), Expect = 7.1e-12
Identity = 41/81 (50.62%), Postives = 56/81 (69.14%), Query Frame = 1


Query: 1  MERKRGALVAMVGLLLIAAFAESTAV---DEVIQLVSDGGNDFPRKMMKG-AEECPRQLV 60
          ME K+ ALVAM+G+LL+A F ES  +   +E+IQLVSDG N++ + + K  A  CPR L+
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60

Query: 61 RCKKDSDCHRACKCMPYHFCG 78
          +CK DSDC+  C C P  +CG
Sbjct: 61 KCKTDSDCYPGCTCKPMGYCG 81

[Close]

BLAST of Cp4.1LG14g04640 vs. NCBI nr
Match: gi|462426|sp|P34950.1|ITR5_LUFAE (RecName: Full=Trypsin inhibitor 5; AltName: Full=TGT-II; AltName: Full=Trypsin inhibitor II; Flags: Precursor)

HSP 1 Score: 70.1 bits (170), Expect = 1.9e-09
Identity = 33/63 (52.38%), Postives = 43/63 (68.25%), Query Frame = 1


Query: 17 IAAFAESTAVDEVIQLVSDGGNDFPRKMMKGAEE--CPRQLVRCKKDSDCHRACKCMPYH 76
          +A+ AES+ V EVI+L+SDGGND PRK+M G     CPR L+ CK D DC   C+C+   
Sbjct: 1  MASVAESSGVVEVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLSNG 60

Query: 77 FCG 78
          +CG
Sbjct: 61 YCG 63

[Close]

BLAST of Cp4.1LG14g04640 vs. NCBI nr
Match: gi|8134511|sp|Q43667.1|ITR1_TRIKI (RecName: Full=Trypsin inhibitor 1; AltName: Full=TTII; AltName: Full=Trypsin inhibitor I; Flags: Precursor)

HSP 1 Score: 69.7 bits (169), Expect = 2.5e-09
Identity = 34/65 (52.31%), Postives = 42/65 (64.62%), Query Frame = 1


Query: 17 IAAFAEST----AVDEVIQLVSDGGNDFPRKMMKGAEECPRQLVRCKKDSDCHRACKCMP 76
          +AAF ES       DEVIQLVSDG N++  KMM+G   CPR L+ CK + DC R CKC+ 
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMEGVVACPRILMPCKVNDDCLRGCKCLS 60

Query: 77 YHFCG 78
            +CG
Sbjct: 61 NGYCG 65

The following BLAST results are available for this feature:

BLAST of Cp4.1LG14g04640 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ITR5_LUFAE	1.2e-11	52.38	Trypsin inhibitor 5 OS=Luffa aegyptiaca PE=1 SV=1	[more]
ITR1_TRIKI	1.6e-11	52.31	Trypsin inhibitor 1 OS=Trichosanthes kirilowii PE=3 SV=1	[more]

BLAST of Cp4.1LG14g04640 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KY34_CUCSA	4.9e-12	50.62	Trypsin inhibitor 1 OS=Cucumis sativus GN=Csa_4G000810 PE=3 SV=1	[more]

BLAST of Cp4.1LG14g04640 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of Cp4.1LG14g04640 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700197603\|gb\|KGN52761.1\|	7.1e-12	50.62	Trypsin inhibitor 1 [Cucumis sativus]	[more]
gi\|462426\|sp\|P34950.1\|ITR5_LUFAE	1.9e-09	52.38	RecName: Full=Trypsin inhibitor 5; AltName: Full=TGT-II; AltName: Full=Trypsin i...	[more]
gi\|8134511\|sp\|Q43667.1\|ITR1_TRIKI	2.5e-09	52.31	RecName: Full=Trypsin inhibitor 1; AltName: Full=TTII; AltName: Full=Trypsin inh...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004866	endopeptidase inhibitor activity
GO:0004867	serine-type endopeptidase inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR011052	Proteinase_amylase_inhib_sf
IPR000737	Prot_inh_squash

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0010466	negative regulation of peptidase activity
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG14g04640.1	Cp4.1LG14g04640.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 51..77 score: 2.
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 51..70 scor
IPR011052	Proteinase/amylase inhibitor domain	unknown	SSF57027	Plant inhibitors of proteinases and amylases	coord: 48..77 score: 5.3
None	No IPR available	SMART	SM00286	PTI_1	coord: 49..77 score: 3.

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cp4.1LG14g04640	CmaCh16G003170	Cucurbita maxima (Rimu)	cmacpeB344
Cp4.1LG14g04640	CmoCh16G003360	Cucurbita moschata (Rifu)	cmocpeB307
Cp4.1LG14g04640	Lsi03G010200	Bottle gourd (USVL1VR-Ls)	cpelsiB168
Cp4.1LG14g04640	MELO3C018005.2	Melon (DHL92) v3.6.1	cpemedB243
Cp4.1LG14g04640	Bhi11G000936	Wax gourd	cpewgoB0266
Cp4.1LG14g04640	CsGy4G000290	Cucumber (Gy14) v2	cgybcpeB485
Cp4.1LG14g04640	Carg19687	Silver-seed gourd	carcpeB0407

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG14g04640	Cucumber (Chinese Long) v3	cpecucB0245
Cp4.1LG14g04640	Cucumber (Chinese Long) v3	cpecucB0263
Cp4.1LG14g04640	Cucumber (Chinese Long) v3	cpecucB0269
Cp4.1LG14g04640	Cucumber (Chinese Long) v3	cpecucB0283
Cp4.1LG14g04640	Wax gourd	cpewgoB0304
Cp4.1LG14g04640	Cucurbita pepo (Zucchini)	cpecpeB234
Cp4.1LG14g04640	Cucumber (Gy14) v1	cgycpeB0215
Cp4.1LG14g04640	Cucurbita maxima (Rimu)	cmacpeB114
Cp4.1LG14g04640	Cucurbita maxima (Rimu)	cmacpeB715
Cp4.1LG14g04640	Cucurbita moschata (Rifu)	cmocpeB092
Cp4.1LG14g04640	Cucurbita moschata (Rifu)	cmocpeB668
Cp4.1LG14g04640	Wild cucumber (PI 183967)	cpecpiB221
Cp4.1LG14g04640	Cucumber (Chinese Long) v2	cpecuB229
Cp4.1LG14g04640	Cucumber (Chinese Long) v2	cpecuB219
Cp4.1LG14g04640	Watermelon (Charleston Gray)	cpewcgB190
Cp4.1LG14g04640	Watermelon (97103) v1	cpewmB204
Cp4.1LG14g04640	Melon (DHL92) v3.5.1	cpemeB200
Cp4.1LG14g04640	Melon (DHL92) v3.5.1	cpemeB201
Cp4.1LG14g04640	Melon (DHL92) v3.5.1	cpemeB182
Cp4.1LG14g04640	Melon (DHL92) v3.5.1	cpemeB208
Cp4.1LG14g04640	Cucumber (Gy14) v2	cgybcpeB492
Cp4.1LG14g04640	Cucumber (Gy14) v2	cgybcpeB747
Cp4.1LG14g04640	Melon (DHL92) v3.6.1	cpemedB220
Cp4.1LG14g04640	Melon (DHL92) v3.6.1	cpemedB242
Cp4.1LG14g04640	Melon (DHL92) v3.6.1	cpemedB250
Cp4.1LG14g04640	Silver-seed gourd	carcpeB0523