CsGy4G000290 (gene) Cucumber (Gy14) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsGy4G000290
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v2)
Description	Trypsin inhibitor 1
Location	Chr4 : 197582 .. 197827 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTGGAAAAAAATTGCTTTAGTTGCAATGATGGGGATGTTGCTTATGGCTACTTTTACAGAGTCAGTCGGTTTGGGCGTGGATGAGGAGATCATTCAATTAGTTTCCGACGGAGTGAATGAGTACTCACAAAATATTGGAAAGGATACTGCACCCGGCTGCCCTCGAATCCTTATGAAGTGTAAAACCGACAGCGATTGCTACCCTGGCTGTACTTGCAAACCGATGGGCTATTGTGGTTGA

mRNA sequence

ATGGAGTGGAAAAAAATTGCTTTAGTTGCAATGATGGGGATGTTGCTTATGGCTACTTTTACAGAGTCAGTCGGTTTGGGCGTGGATGAGGAGATCATTCAATTAGTTTCCGACGGAGTGAATGAGTACTCACAAAATATTGGAAAGGATACTGCACCCGGCTGCCCTCGAATCCTTATGAAGTGTAAAACCGACAGCGATTGCTACCCTGGCTGTACTTGCAAACCGATGGGCTATTGTGGTTGA

Coding sequence (CDS)

ATGGAGTGGAAAAAAATTGCTTTAGTTGCAATGATGGGGATGTTGCTTATGGCTACTTTTACAGAGTCAGTCGGTTTGGGCGTGGATGAGGAGATCATTCAATTAGTTTCCGACGGAGTGAATGAGTACTCACAAAATATTGGAAAGGATACTGCACCCGGCTGCCCTCGAATCCTTATGAAGTGTAAAACCGACAGCGATTGCTACCCTGGCTGTACTTGCAAACCGATGGGCTATTGTGGTTGA

Protein sequence

MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILMKCKTDSDCYPGCTCKPMGYCG

Homology

[Close]

BLAST of CsGy4G000290 vs. NCBI nr
Match: KGN52761.1 (Trypsin inhibitor 1 [Cucumis sativus])

HSP 1 Score: 171.8 bits (434), Expect = 9.6e-40
Identity = 81/81 (100.00%), Postives = 81/81 (100.00%), Query Frame = 0


Query: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60
          MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60

Query: 61 KCKTDSDCYPGCTCKPMGYCG 82
          KCKTDSDCYPGCTCKPMGYCG
Sbjct: 61 KCKTDSDCYPGCTCKPMGYCG 81

[Close]

BLAST of CsGy4G000290 vs. NCBI nr
Match: XP_023552845.1 (uncharacterized protein LOC111810370 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 97.8 bits (242), Expect = 1.8e-17
Identity = 46/81 (56.79%), Postives = 62/81 (76.54%), Query Frame = 0


Query: 1   MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60
           MEWK+ AL+AM+G+LLMA F +S  +    E+IQLVSDGVN++ + +  + A GCPRILM
Sbjct: 85  MEWKRGALIAMVGLLLMAAFADSAAVH-GGEVIQLVSDGVNDFPRKM-MNAALGCPRILM 144

Query: 61  KCKTDSDCYPGCTCKPMGYCG 82
           +C+ DSDC PGC C+P G+CG
Sbjct: 145 ECEVDSDCLPGCVCRPNGFCG 163

[Close]

BLAST of CsGy4G000290 vs. NCBI nr
Match: XP_022984819.1 (trypsin inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 96.7 bits (239), Expect = 3.9e-17
Identity = 46/81 (56.79%), Postives = 61/81 (75.31%), Query Frame = 0


Query: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60
          MEWK+ AL+AM+G+LLMA F ES  +    E+IQLVSDGVN++ + +  +   GCPRILM
Sbjct: 1  MEWKRGALIAMVGLLLMAAFAESAAVH-GGEVIQLVSDGVNDFPRKM-MNGVQGCPRILM 60

Query: 61 KCKTDSDCYPGCTCKPMGYCG 82
          +C+ DSDC PGC C+P G+CG
Sbjct: 61 ECELDSDCLPGCICRPNGFCG 79

[Close]

BLAST of CsGy4G000290 vs. NCBI nr
Match: Q43667.1 (RecName: Full=Trypsin inhibitor 1; AltName: Full=TTII; AltName: Full=Trypsin inhibitor I; Flags: Precursor >CAA57704.1 Trichosanthes trypsin inhibitor-I [Trichosanthes kirilowii])

HSP 1 Score: 72.0 bits (175), Expect = 1.0e-09
Identity = 36/66 (54.55%), Postives = 42/66 (63.64%), Query Frame = 0


Query: 17 MATFTESVGLGVD-EEIIQLVSDGVNEYSQNIGKDTAPGCPRILMKCKTDSDCYPGCTCK 76
          MA F ES   G   +E+IQLVSDGVNEYS+ +  +    CPRILM CK + DC  GC C 
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKM-MEGVVACPRILMPCKVNDDCLRGCKCL 60

Query: 77 PMGYCG 82
            GYCG
Sbjct: 61 SNGYCG 65

[Close]

BLAST of CsGy4G000290 vs. NCBI nr
Match: KGN52760.1 (hypothetical protein Csa_4G000805 [Cucumis sativus])

HSP 1 Score: 64.7 bits (156), Expect = 1.7e-07
Identity = 31/81 (38.27%), Postives = 47/81 (58.02%), Query Frame = 0


Query: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60
          MEWKKI ++A++GML+MA   E     ++ E ++ ++   NE  + +  +    CPRIL 
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNK--NELPRKMMNEETRMCPRILE 60

Query: 61 KCKTDSDCYPGCTCKPMGYCG 82
          +C TD DC   C C   G+CG
Sbjct: 61 ECTTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of CsGy4G000290 vs. Swiss-Prot
Match: sp|Q43667|ITR1_TRIKI (Trypsin inhibitor 1 OS=Trichosanthes kirilowii OX=3677 PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 3.4e-12
Identity = 36/66 (54.55%), Postives = 42/66 (63.64%), Query Frame = 0


Query: 17 MATFTESVGLGVD-EEIIQLVSDGVNEYSQNIGKDTAPGCPRILMKCKTDSDCYPGCTCK 76
          MA F ES   G   +E+IQLVSDGVNEYS+ +  +    CPRILM CK + DC  GC C 
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKM-MEGVVACPRILMPCKVNDDCLRGCKCL 60

Query: 77 PMGYCG 82
            GYCG
Sbjct: 61 SNGYCG 65

[Close]

BLAST of CsGy4G000290 vs. Swiss-Prot
Match: sp|P34950|ITR5_LUFAE (Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.3e-08
Identity = 32/66 (48.48%), Postives = 39/66 (59.09%), Query Frame = 0


Query: 17 MATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPG-CPRILMKCKTDSDCYPGCTCK 76
          MA+  ES G+    E+I+L+SDG N+  + I      G CPRILM CKTD DC   C C 
Sbjct: 1  MASVAESSGV---VEVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCL 60

Query: 77 PMGYCG 82
            GYCG
Sbjct: 61 SNGYCG 63

[Close]

BLAST of CsGy4G000290 vs. Swiss-Prot
Match: sp|P84452|ITR5_SECED (Trypsin inhibitor 5 OS=Sechium edule OX=184140 PE=1 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 1.5e-07
Identity = 20/27 (74.07%), Postives = 24/27 (88.89%), Query Frame = 0


Query: 55 CPRILMKCKTDSDCYPGCTCKPMGYCG 82
          CPRILMKCK D+DC+P CTC+P G+CG
Sbjct: 1  CPRILMKCKLDTDCFPTCTCRPSGFCG 27

[Close]

BLAST of CsGy4G000290 vs. Swiss-Prot
Match: sp|P12071|ITR2_ECBEL (Trypsin inhibitor 2 OS=Ecballium elaterium OX=3679 PE=1 SV=2)

HSP 1 Score: 53.5 bits (127), Expect = 1.2e-06
Identity = 20/28 (71.43%), Postives = 22/28 (78.57%), Query Frame = 0


Query: 54 GCPRILMKCKTDSDCYPGCTCKPMGYCG 82
          GCPRILM+CK DSDC  GC C P G+CG
Sbjct: 1  GCPRILMRCKQDSDCLAGCVCGPNGFCG 28

[Close]

BLAST of CsGy4G000290 vs. Swiss-Prot
Match: sp|P11968|ITR2_BRYDI (Trypsin inhibitor 2 OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 1.6e-06
Identity = 20/28 (71.43%), Postives = 22/28 (78.57%), Query Frame = 0


Query: 54 GCPRILMKCKTDSDCYPGCTCKPMGYCG 82
          GCPRILM+CK DSDC  GC C+  GYCG
Sbjct: 2  GCPRILMRCKRDSDCLAGCVCQKNGYCG 29

[Close]

BLAST of CsGy4G000290 vs. TrEMBL
Match: tr|A0A0A0KY34|A0A0A0KY34_CUCSA (Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 6.4e-40
Identity = 81/81 (100.00%), Postives = 81/81 (100.00%), Query Frame = 0


Query: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60
          MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60

Query: 61 KCKTDSDCYPGCTCKPMGYCG 82
          KCKTDSDCYPGCTCKPMGYCG
Sbjct: 61 KCKTDSDCYPGCTCKPMGYCG 81

[Close]

BLAST of CsGy4G000290 vs. TrEMBL
Match: tr|A0A0A0KT01|A0A0A0KT01_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000805 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.1e-07
Identity = 31/81 (38.27%), Postives = 47/81 (58.02%), Query Frame = 0


Query: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60
          MEWKKI ++A++GML+MA   E     ++ E ++ ++   NE  + +  +    CPRIL 
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNK--NELPRKMMNEETRMCPRILE 60

Query: 61 KCKTDSDCYPGCTCKPMGYCG 82
          +C TD DC   C C   G+CG
Sbjct: 61 ECTTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of CsGy4G000290 vs. TrEMBL
Match: tr|J3R2I9|J3R2I9_MOMCO (Two inhibitor peptide topologies 2 OS=Momordica cochinchinensis OX=3674 GN=TIPTOP2 PE=2 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 1.0e-05
Identity = 35/81 (43.21%), Postives = 47/81 (58.02%), Query Frame = 0


Query: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60
          ME KKI  V ++ M+L+AT   S G   D + I+L+SDG  +   N G      CP+IL 
Sbjct: 1  MESKKILPVVLVAMMLVAT---STGFN-DGDTIELISDGRAQIDINGGV-----CPKILQ 60

Query: 61 KCKTDSDCYPGCTCKPMGYCG 82
          +C+ DSDC   C C+  GYCG
Sbjct: 61 RCRRDSDCPGACICRGNGYCG 72

[Close]

BLAST of CsGy4G000290 vs. TrEMBL
Match: tr|J3RCD6|J3RCD6_MOMCO (Two inhibitor peptide topologies 1 OS=Momordica cochinchinensis OX=3674 GN=TIPTOP1 PE=2 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 1.0e-05
Identity = 35/81 (43.21%), Postives = 47/81 (58.02%), Query Frame = 0


Query: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60
          ME KKI  V ++ M+L+AT   S G   D + I+L+SDG  +   N G      CP+IL 
Sbjct: 1  MESKKILPVVLVAMMLVAT---STGFN-DGDTIELISDGRAQIDINGGV-----CPKILQ 60

Query: 61 KCKTDSDCYPGCTCKPMGYCG 82
          +C+ DSDC   C C+  GYCG
Sbjct: 61 RCRRDSDCPGACICRGNGYCG 72

[Close]

BLAST of CsGy4G000290 vs. TrEMBL
Match: tr|A0A0A0KV23|A0A0A0KV23_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000835 PE=3 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 1.0e-05
Identity = 38/98 (38.78%), Postives = 53/98 (54.08%), Query Frame = 0


Query: 1  MEWKKIALVAMMGMLLMATFTESVGLGV--DEEIIQLVSDGVNEYSQNIG---------- 60
          ME KKI +VA++  +++     S    V  D++ I+LVSDG  +  Q++           
Sbjct: 1  MESKKIVMVAIVATIIVQVAMSSSASFVVDDKDWIKLVSDGRAQADQDMNNGCPRKMMIK 60

Query: 61 ----KDTAPGCPRILMKCKTDSDCYPGCTC-KPMGYCG 82
              K+    CPRILMKCK DSDC PGC C + + YCG
Sbjct: 61 GGVFKEEEMMCPRILMKCKHDSDCLPGCVCLEHIEYCG 98

The following BLAST results are available for this feature:

BLAST of CsGy4G000290 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN52761.1	9.6e-40	100.00	Trypsin inhibitor 1 [Cucumis sativus]	[more]
XP_023552845.1	1.8e-17	56.79	uncharacterized protein LOC111810370 [Cucurbita pepo subsp. pepo]	[more]
XP_022984819.1	3.9e-17	56.79	trypsin inhibitor 1-like [Cucurbita maxima]	[more]
Q43667.1	1.0e-09	54.55	RecName: Full=Trypsin inhibitor 1; AltName: Full=TTII; AltName: Full=Trypsin inh...	[more]
KGN52760.1	1.7e-07	38.27	hypothetical protein Csa_4G000805 [Cucumis sativus]	[more]

BLAST of CsGy4G000290 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of CsGy4G000290 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q43667\|ITR1_TRIKI	3.4e-12	54.55	Trypsin inhibitor 1 OS=Trichosanthes kirilowii OX=3677 PE=3 SV=1	[more]
sp\|P34950\|ITR5_LUFAE	1.3e-08	48.48	Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
sp\|P84452\|ITR5_SECED	1.5e-07	74.07	Trypsin inhibitor 5 OS=Sechium edule OX=184140 PE=1 SV=1	[more]
sp\|P12071\|ITR2_ECBEL	1.2e-06	71.43	Trypsin inhibitor 2 OS=Ecballium elaterium OX=3679 PE=1 SV=2	[more]
sp\|P11968\|ITR2_BRYDI	1.6e-06	71.43	Trypsin inhibitor 2 OS=Bryonia dioica OX=3652 PE=1 SV=1	[more]

BLAST of CsGy4G000290 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KY34\|A0A0A0KY34_CUCSA	6.4e-40	100.00	Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1	[more]
tr\|A0A0A0KT01\|A0A0A0KT01_CUCSA	1.1e-07	38.27	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000805 PE=3 SV=1	[more]
tr\|J3R2I9\|J3R2I9_MOMCO	1.0e-05	43.21	Two inhibitor peptide topologies 2 OS=Momordica cochinchinensis OX=3674 GN=TIPTO...	[more]
tr\|J3RCD6\|J3RCD6_MOMCO	1.0e-05	43.21	Two inhibitor peptide topologies 1 OS=Momordica cochinchinensis OX=3674 GN=TIPTO...	[more]
tr\|A0A0A0KV23\|A0A0A0KV23_CUCSA	1.0e-05	38.78	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000835 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004866	endopeptidase inhibitor activity
GO:0004867	serine-type endopeptidase inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR011052	Proteinase_amylase_inhib_sf
IPR000737	Prot_inh_squash

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy4G000290.1	CsGy4G000290.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00286	PTI_1	coord: 53..81 e-value: 3.2E-8 score: 43.3
None	No IPR available	GENE3D	G3DSA:3.10.450.30		coord: 24..81 e-value: 3.5E-10 score: 42.0
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 55..81 e-value: 2.8E-12 score: 46.3
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 55..74
IPR000737	Proteinase inhibitor I7, squash	CDD	cd00150	PlantTI	coord: 55..81 e-value: 2.91595E-9 score: 46.9871
IPR011052	Proteinase/amylase inhibitor domain superfamily	SUPERFAMILY	SSF57027	Plant inhibitors of proteinases and amylases	coord: 52..81

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CsGy4G000290	Csa4G000810	Cucumber (Chinese Long) v2	cgybcuB165
CsGy4G000290	MELO3C018005	Melon (DHL92) v3.5.1	cgybmeB269
CsGy4G000290	CSPI04G00330	Wild cucumber (PI 183967)	cgybcpiB168
CsGy4G000290	CmaCh16G003170	Cucurbita maxima (Rimu)	cgybcmaB494
CsGy4G000290	Lsi03G010200	Bottle gourd (USVL1VR-Ls)	cgyblsiB257
CsGy4G000290	Cp4.1LG14g04640	Cucurbita pepo (Zucchini)	cgybcpeB485
CsGy4G000290	MELO3C018005.2	Melon (DHL92) v3.6.1	cgybmedB266
CsGy4G000290	Bhi11G000941	Wax gourd	cgybwgoB342

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CsGy4G000290	Cucumber (Chinese Long) v3	cgybcucB193
CsGy4G000290	Cucumber (Chinese Long) v3	cgybcucB171
CsGy4G000290	Cucumber (Chinese Long) v3	cgybcucB177
CsGy4G000290	Watermelon (97103) v2	cgybwmbB259
CsGy4G000290	Cucumber (Gy14) v2	cgybcgybB122
CsGy4G000290	Cucurbita maxima (Rimu)	cgybcmaB543
CsGy4G000290	Cucurbita moschata (Rifu)	cgybcmoB465
CsGy4G000290	Cucurbita moschata (Rifu)	cgybcmoB517
CsGy4G000290	Cucurbita pepo (Zucchini)	cgybcpeB504
CsGy4G000290	Cucumber (Chinese Long) v2	cgybcuB177
CsGy4G000290	Melon (DHL92) v3.5.1	cgybmeB267
CsGy4G000290	Watermelon (Charleston Gray)	cgybwcgB251
CsGy4G000290	Watermelon (97103) v1	cgybwmB269
CsGy4G000290	Wild cucumber (PI 183967)	cgybcpiB178
CsGy4G000290	Silver-seed gourd	carcgybB0309
CsGy4G000290	Silver-seed gourd	carcgybB0383