Carg25067 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg25067
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	WRKY family transcription factor
Location	Cucurbita_argyrosperma_scaffold_201 : 374188 .. 375645 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGGATCACAAAGATCAGAGCTTCCCAATTATCAACTTCAAGTCTCCTTCCTCTCAAATGCACCGCACCCTCAGCCCATCCACGAAATGGGGTTTCTCCATTTTGAAGACCACAATCAGGTTTTGAGCTTCTTGGCACCTAATAACAACGCCGCCACAACCACTACTGCACCACCCACCACCACCGACACCACCACCACCACCACCACCGCCATGCCTTCTGCCTTCACTACCCCTCCTCACGCGCACCTCCTTACTAGACCCTCTTGGAGCAACGACCAGGTGGGGTTTTTAATCAATCCATGTAAAAAAGGATGGATCTTGGTTATTAAGATTTGGGTTTTGTTTCATCAGGTGGGAACACTAGATCCAAAGCCCGGTGGCGATGAAAACTGCACTGGTAGCACCAGCGACGGCACCAATTCATGGTACCTACACACACACACACACACACACACACACCCCTTTCCCCTACCTTTCATATCACTTCATTATCATTACATTAACCTAACAATTGGTTACTCAAAAATTCATTCCTGAGTCGCAGGTGGAGAAACTCCAACGCAGATAAGACCAAAGTTAAGGTCAGGAGAAAGCTGAGAGAACCGCGTTTCTGTTTCCAAACGAGAAGCGATGTCGATGTCCTCGACGACGGCTACAAATGGAGAAAATACGGCCAAAAAGTCGTCAAAAACAGTCTTCATCCACGGTACCACCTCATTTCAATTCTTCTTCTAATTGATACAACTAGCTACCGTTAGGAGTATGATATTATCCACTTTGAAATCGACTTCTAAATTGAAACCCTGATGAGTAATTTAGAACACCCACATCCAATAATGGGAAATAGATTGATTAATTATGGCCAAAAAAAACCCTTGAAGAGTGATGTGCATGTTTTGTTAGATATGGGGAATATATAGTGTATGATGAGGTGAATATAGAAGCGACAATGAGAGGGATAGCAAAAGAGGGTAATAATACTAACAGTTGAGGCTGAGTAGTAGGCAGATGAGGAATGATTATGTTATATTCTCTCTGAGCCTGAGAATTAGCAGAGGCAGGCATCATTATTGATTTGGGGTATTTGGGGTATTTGGGGGATAGGTGGCCGCCCCCCCCCCAAGGGCATGTCCAAGCCCACAACACAACCAGTCAGTCATGCATTTCATTCGCCTGTTTCTTGACTACTTTTTGGTCATCAGCGTTGTCCGTTTGTTTAGGGTTGTTGTAATGATGATGATGATGAATAAATAGTTAAAGAAAAAAAGATATGATGCTGTTGCATGCAGAAGCTACTACCGTTGCACGCATAGCAACTGCCGTGTCAAGAAGAGGGTGGAGCGGCTGTCGGAGGATTGCCGGATGGTCATAACTACATACGAAGGTAGACACAATCACTCCCCTTGCGACGACTCCAATTCTTCCGACCATGAACCCTTTACCTCCTTCTGA

mRNA sequence

ATGGAAGGATCACAAAGATCAGAGCTTCCCAATTATCAACTTCAAGTCTCCTTCCTCTCAAATGCACCGCACCCTCAGCCCATCCACGAAATGGGGTTTCTCCATTTTGAAGACCACAATCAGGTTTTGAGCTTCTTGGCACCTAATAACAACGCCGCCACAACCACTACTGCACCACCCACCACCACCGACACCACCACCACCACCACCACCGCCATGCCTTCTGCCTTCACTACCCCTCCTCACGCGCACCTCCTTACTAGACCCTCTTGGAGCAACGACCAGGTGGGAACACTAGATCCAAAGCCCGGTGGCGATGAAAACTGCACTGGTAGCACCAGCGACGGCACCAATTCATGGTGGAGAAACTCCAACGCAGATAAGACCAAAGTTAAGGTCAGGAGAAAGCTGAGAGAACCGCGTTTCTGTTTCCAAACGAGAAGCGATGTCGATGTCCTCGACGACGGCTACAAATGGAGAAAATACGGCCAAAAAGTCGTCAAAAACAGTCTTCATCCACGAAGCTACTACCGTTGCACGCATAGCAACTGCCGTGTCAAGAAGAGGGTGGAGCGGCTGTCGGAGGATTGCCGGATGGTCATAACTACATACGAAGGTAGACACAATCACTCCCCTTGCGACGACTCCAATTCTTCCGACCATGAACCCTTTACCTCCTTCTGA

Coding sequence (CDS)

ATGGAAGGATCACAAAGATCAGAGCTTCCCAATTATCAACTTCAAGTCTCCTTCCTCTCAAATGCACCGCACCCTCAGCCCATCCACGAAATGGGGTTTCTCCATTTTGAAGACCACAATCAGGTTTTGAGCTTCTTGGCACCTAATAACAACGCCGCCACAACCACTACTGCACCACCCACCACCACCGACACCACCACCACCACCACCACCGCCATGCCTTCTGCCTTCACTACCCCTCCTCACGCGCACCTCCTTACTAGACCCTCTTGGAGCAACGACCAGGTGGGAACACTAGATCCAAAGCCCGGTGGCGATGAAAACTGCACTGGTAGCACCAGCGACGGCACCAATTCATGGTGGAGAAACTCCAACGCAGATAAGACCAAAGTTAAGGTCAGGAGAAAGCTGAGAGAACCGCGTTTCTGTTTCCAAACGAGAAGCGATGTCGATGTCCTCGACGACGGCTACAAATGGAGAAAATACGGCCAAAAAGTCGTCAAAAACAGTCTTCATCCACGAAGCTACTACCGTTGCACGCATAGCAACTGCCGTGTCAAGAAGAGGGTGGAGCGGCTGTCGGAGGATTGCCGGATGGTCATAACTACATACGAAGGTAGACACAATCACTCCCCTTGCGACGACTCCAATTCTTCCGACCATGAACCCTTTACCTCCTTCTGA

Protein sequence

MEGSQRSELPNYQLQVSFLSNAPHPQPIHEMGFLHFEDHNQVLSFLAPNNNAATTTTAPPTTTDTTTTTTTAMPSAFTTPPHAHLLTRPSWSNDQVGTLDPKPGGDENCTGSTSDGTNSWWRNSNADKTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSDHEPFTSF

Homology

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BLAST of Carg25067 vs. NCBI nr
Match: XP_022935668.1 (probable WRKY transcription factor 12 [Cucurbita moschata])

HSP 1 Score: 396.0 bits (1016), Expect = 8.8e-107
Identity = 222/227 (97.80%), Postives = 223/227 (98.24%), Query Frame = 0


Query: 1   MEGSQRSELPNYQLQVSFLSNAPHPQPIHEMGFLHFEDHNQVLSFLAPXXXXXXXXXXXX 60
           MEGSQRSELPNYQLQVSFLSNAPHPQPIHEMGFLHFEDHNQVLSFLAP   XXXXXXXXX
Sbjct: 1   MEGSQRSELPNYQLQVSFLSNAPHPQPIHEMGFLHFEDHNQVLSFLAP---XXXXXXXXX 60

Query: 61  XXXXXXXXXXXXXXXXXXXXXHAHLLTRPSWSNDQVGTLDPKPGGDENCTGSTSDGTNSW 120
           XXXXXXXXXXXXXXXXXXXX HAHLL+RPSWSNDQVGTLDPKPGGDENCTGSTSDGTNSW
Sbjct: 61  XXXXXXXXXXXXXXXXXXXXPHAHLLSRPSWSNDQVGTLDPKPGGDENCTGSTSDGTNSW 120

Query: 121 WRNSNADKTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCT 180
           WRNSNADKTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCT
Sbjct: 121 WRNSNADKTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCT 180

Query: 181 HSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSDHEPFTSF 228
           HSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSDHEPFTSF
Sbjct: 181 HSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSDHEPFTSF 224

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BLAST of Carg25067 vs. NCBI nr
Match: XP_023538960.1 (probable WRKY transcription factor 12 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 393.7 bits (1010), Expect = 4.4e-106
Identity = 224/227 (98.68%), Postives = 226/227 (99.56%), Query Frame = 0


Query: 1   MEGSQRSELPNYQLQVSFLSNAPHPQPIHEMGFLHFEDHNQVLSFLAPXXXXXXXXXXXX 60
           MEGSQRSELPNYQLQVSFLSNAP+PQPIHEMGFLHFEDHNQVLSF+AP XXXXXXXXXXX
Sbjct: 1   MEGSQRSELPNYQLQVSFLSNAPNPQPIHEMGFLHFEDHNQVLSFVAP-XXXXXXXXXXX 60

Query: 61  XXXXXXXXXXXXXXXXXXXXXHAHLLTRPSWSNDQVGTLDPKPGGDENCTGSTSDGTNSW 120
           XXXXXXXXXXXXXXXXXXXXXHAHLLTRPSWSNDQVGTLDPKPGGDENCTGSTSDGTNSW
Sbjct: 61  XXXXXXXXXXXXXXXXXXXXXHAHLLTRPSWSNDQVGTLDPKPGGDENCTGSTSDGTNSW 120

Query: 121 WRNSNADKTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCT 180
           WRNSNADKTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCT
Sbjct: 121 WRNSNADKTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCT 180

Query: 181 HSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSDHEPFTSF 228
           HSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSDHEPFTSF
Sbjct: 181 HSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSDHEPFTSF 226

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BLAST of Carg25067 vs. NCBI nr
Match: XP_022974266.1 (probable WRKY transcription factor 12 isoform X2 [Cucurbita maxima])

HSP 1 Score: 392.9 bits (1008), Expect = 7.4e-106
Identity = 222/228 (97.37%), Postives = 225/228 (98.68%), Query Frame = 0


Query: 1   MEGSQRSELPNYQLQVSFLSNAPHPQPIHEMGFLHFEDHNQVLSFLAP-XXXXXXXXXXX 60
           MEGSQRSELPNYQLQVSFLSNAPHPQPIHEMGF+HFEDHNQVLSFLAP  XXXXXXXXXX
Sbjct: 1   MEGSQRSELPNYQLQVSFLSNAPHPQPIHEMGFVHFEDHNQVLSFLAPNSXXXXXXXXXX 60

Query: 61  XXXXXXXXXXXXXXXXXXXXXXHAHLLTRPSWSNDQVGTLDPKPGGDENCTGSTSDGTNS 120
           XXXXXXXXXXXXXXXXXXXXX HAHLL+RPSWSNDQVGTLDPKPGGDENCTGSTSDG+NS
Sbjct: 61  XXXXXXXXXXXXXXXXXXXXXPHAHLLSRPSWSNDQVGTLDPKPGGDENCTGSTSDGSNS 120

Query: 121 WWRNSNADKTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRC 180
           WWRNSNADKTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRC
Sbjct: 121 WWRNSNADKTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRC 180

Query: 181 THSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSDHEPFTSF 228
           THSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSDHEPFTSF
Sbjct: 181 THSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSDHEPFTSF 228

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BLAST of Carg25067 vs. NCBI nr
Match: XP_023538959.1 (probable WRKY transcription factor 12 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 383.6 bits (984), Expect = 4.5e-103
Identity = 223/229 (97.38%), Postives = 225/229 (98.25%), Query Frame = 0


Query: 1   MEGSQRSELPNYQLQVSFLSNAPHPQPIHEMGFLHFEDHNQVLSFLAPXXXXXXXXXXXX 60
           MEGSQRSELPNYQLQVSFLSNAP+PQPIHEMGFLHFEDHNQVLSF+AP XXXXXXXXXXX
Sbjct: 1   MEGSQRSELPNYQLQVSFLSNAPNPQPIHEMGFLHFEDHNQVLSFVAP-XXXXXXXXXXX 60

Query: 61  XXXXXXXXXXXXXXXXXXXXXHAHLLTRPSWSNDQVGTLDPKPGGDENCTGSTSDGTNSW 120
           XXXXXXXXXXXXXXXXXXXXXHAHLLTRPSWSNDQVGTLDPKPGGDENCTGSTSDGTNS 
Sbjct: 61  XXXXXXXXXXXXXXXXXXXXXHAHLLTRPSWSNDQVGTLDPKPGGDENCTGSTSDGTNSC 120

Query: 121 --WRNSNADKTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYR 180
             WRNSNADKTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYR
Sbjct: 121 ARWRNSNADKTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYR 180

Query: 181 CTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSDHEPFTSF 228
           CTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSDHEPFTSF
Sbjct: 181 CTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSDHEPFTSF 228

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BLAST of Carg25067 vs. NCBI nr
Match: XP_022974265.1 (probable WRKY transcription factor 12 isoform X1 [Cucurbita maxima])

HSP 1 Score: 382.9 bits (982), Expect = 7.7e-103
Identity = 221/230 (96.09%), Postives = 224/230 (97.39%), Query Frame = 0


Query: 1   MEGSQRSELPNYQLQVSFLSNAPHPQPIHEMGFLHFEDHNQVLSFLAP-XXXXXXXXXXX 60
           MEGSQRSELPNYQLQVSFLSNAPHPQPIHEMGF+HFEDHNQVLSFLAP  XXXXXXXXXX
Sbjct: 1   MEGSQRSELPNYQLQVSFLSNAPHPQPIHEMGFVHFEDHNQVLSFLAPNSXXXXXXXXXX 60

Query: 61  XXXXXXXXXXXXXXXXXXXXXXHAHLLTRPSWSNDQVGTLDPKPGGDENCTGSTSDGTNS 120
           XXXXXXXXXXXXXXXXXXXXX HAHLL+RPSWSNDQVGTLDPKPGGDENCTGSTSDG+NS
Sbjct: 61  XXXXXXXXXXXXXXXXXXXXXPHAHLLSRPSWSNDQVGTLDPKPGGDENCTGSTSDGSNS 120

Query: 121 W--WRNSNADKTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYY 180
              WRNSNADKTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYY
Sbjct: 121 CGRWRNSNADKTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYY 180

Query: 181 RCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSDHEPFTSF 228
           RCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSDHEPFTSF
Sbjct: 181 RCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSDHEPFTSF 230

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BLAST of Carg25067 vs. TAIR10
Match: AT2G44745.1 (WRKY family transcription factor)

HSP 1 Score: 191.0 bits (484), Expect = 7.8e-49
Identity = 88/100 (88.00%), Postives = 93/100 (93.00%), Query Frame = 0


Query: 128 KTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHSNCRVK 187
           K KVK+RRKLREPRFCFQT+SDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTH+NCRVK
Sbjct: 119 KNKVKIRRKLREPRFCFQTKSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHNNCRVK 178

Query: 188 KRVERLSEDCRMVITTYEGRHNHSPCDDSNSSDHEPFTSF 228
           KRVERLSEDCRMVITTYEGRHNH P DDS S DH+  +SF
Sbjct: 179 KRVERLSEDCRMVITTYEGRHNHIPSDDSTSPDHDCLSSF 218

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BLAST of Carg25067 vs. TAIR10
Match: AT4G39410.1 (WRKY DNA-binding protein 13)

HSP 1 Score: 151.0 bits (380), Expect = 8.9e-37
Identity = 75/110 (68.18%), Postives = 87/110 (79.09%), Query Frame = 0


Query: 124 SNADKTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHSN 183
           S+    K+K RRK+REPRFCF+T S+VDVLDDGY+WRKYGQKVVKN+ HPRSYYRCT   
Sbjct: 193 SSLKMKKLKTRRKVREPRFCFKTLSEVDVLDDGYRWRKYGQKVVKNTQHPRSYYRCTQDK 252

Query: 184 CRVKKRVERLSEDCRMVITTYEGRHNHSPC----DDSNSSD--HEPFTSF 228
           CRVKKRVERL++D RMVITTYEGRH HSP     DDS S+   H P ++F
Sbjct: 253 CRVKKRVERLADDPRMVITTYEGRHLHSPSNHLDDDSLSTSHLHPPLSNF 302

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BLAST of Carg25067 vs. TAIR10
Match: AT2G46130.1 (WRKY DNA-binding protein 43)

HSP 1 Score: 121.3 bits (303), Expect = 7.6e-28
Identity = 53/81 (65.43%), Postives = 68/81 (83.95%), Query Frame = 0


Query: 135 RKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHSNCRVKKRVERLS 194
           +K++ PRF F+T+SD D+LDDGY+WRKYGQK VKNSL+PRSYYRCT   C VKK+V+RLS
Sbjct: 11  KKMKNPRFSFRTKSDADILDDGYRWRKYGQKSVKNSLYPRSYYRCTQHMCNVKKQVQRLS 70

Query: 195 EDCRMVITTYEGRHNHSPCDD 216
           ++  +V TTYEG HNH PC++
Sbjct: 71  KETSIVETTYEGIHNH-PCEE 90

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BLAST of Carg25067 vs. TAIR10
Match: AT5G41570.1 (WRKY DNA-binding protein 24)

HSP 1 Score: 119.4 bits (298), Expect = 2.9e-27
Identity = 53/85 (62.35%), Postives = 68/85 (80.00%), Query Frame = 0


Query: 130 KVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHSNCRVKKR 189
           ++K +R  + PR  F TRSD DVLDDGY+WRKYGQK VK++ HPRSYYRCT+  C VKK+
Sbjct: 74  ELKEKRSRKVPRIAFHTRSDDDVLDDGYRWRKYGQKSVKHNAHPRSYYRCTYHTCNVKKQ 133

Query: 190 VERLSEDCRMVITTYEGRHNHSPCD 215
           V+RL++D  +V+TTYEG HNH PC+
Sbjct: 134 VQRLAKDPNVVVTTYEGVHNH-PCE 157

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BLAST of Carg25067 vs. TAIR10
Match: AT1G69310.1 (WRKY DNA-binding protein 57)

HSP 1 Score: 119.0 bits (297), Expect = 3.8e-27
Identity = 53/76 (69.74%), Postives = 64/76 (84.21%), Query Frame = 0


Query: 135 RKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHSNCRVKKRVERLS 194
           +++R+PRF F T+SDVD L+DGY+WRKYGQK VKNS  PRSYYRCT+S C VKKRVER S
Sbjct: 128 KRIRQPRFAFMTKSDVDNLEDGYRWRKYGQKAVKNSPFPRSYYRCTNSRCTVKKRVERSS 187

Query: 195 EDCRMVITTYEGRHNH 211
           +D  +VITTYEG+H H
Sbjct: 188 DDPSIVITTYEGQHCH 203

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BLAST of Carg25067 vs. Swiss-Prot
Match: sp|Q93WY4|WRK12_ARATH (Probable WRKY transcription factor 12 OS=Arabidopsis thaliana OX=3702 GN=WRKY12 PE=2 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.4e-47
Identity = 88/100 (88.00%), Postives = 93/100 (93.00%), Query Frame = 0


Query: 128 KTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHSNCRVK 187
           K KVK+RRKLREPRFCFQT+SDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTH+NCRVK
Sbjct: 119 KNKVKIRRKLREPRFCFQTKSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHNNCRVK 178

Query: 188 KRVERLSEDCRMVITTYEGRHNHSPCDDSNSSDHEPFTSF 228
           KRVERLSEDCRMVITTYEGRHNH P DDS S DH+  +SF
Sbjct: 179 KRVERLSEDCRMVITTYEGRHNHIPSDDSTSPDHDCLSSF 218

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BLAST of Carg25067 vs. Swiss-Prot
Match: sp|Q9SVB7|WRK13_ARATH (Probable WRKY transcription factor 13 OS=Arabidopsis thaliana OX=3702 GN=WRKY13 PE=2 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.6e-35
Identity = 75/110 (68.18%), Postives = 87/110 (79.09%), Query Frame = 0


Query: 124 SNADKTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHSN 183
           S+    K+K RRK+REPRFCF+T S+VDVLDDGY+WRKYGQKVVKN+ HPRSYYRCT   
Sbjct: 193 SSLKMKKLKTRRKVREPRFCFKTLSEVDVLDDGYRWRKYGQKVVKNTQHPRSYYRCTQDK 252

Query: 184 CRVKKRVERLSEDCRMVITTYEGRHNHSPC----DDSNSSD--HEPFTSF 228
           CRVKKRVERL++D RMVITTYEGRH HSP     DDS S+   H P ++F
Sbjct: 253 CRVKKRVERLADDPRMVITTYEGRHLHSPSNHLDDDSLSTSHLHPPLSNF 302

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BLAST of Carg25067 vs. Swiss-Prot
Match: sp|Q6B6R4|WRK24_ORYSI (WRKY transcription factor WRKY24 OS=Oryza sativa subsp. indica OX=39946 GN=WRKY24 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 6.1e-27
Identity = 60/124 (48.39%), Postives = 81/124 (65.32%), Query Frame = 0


Query: 90  SWSNDQVGTLDPKP---GGDENCTGSTSDGTNSWWRNSNADKTKVKVRRKLREPRFCFQT 149
           S+ +D++G   P+    GGDE         +  W ++ + +   +   R +REPR   QT
Sbjct: 320 SFGDDEIGVGSPRAGNGGGDE--FDDDEPDSKRWRKDGDGEGISMAGNRTVREPRVVVQT 379

Query: 150 RSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEG 209
            SD+D+LDDGY+WRKYGQKVVK + +PRSYY+CT + C V+K VER S D R VITTYEG
Sbjct: 380 MSDIDILDDGYRWRKYGQKVVKGNPNPRSYYKCTTAGCPVRKHVERASHDLRAVITTYEG 439

Query: 210 RHNH 211
           +HNH
Sbjct: 440 KHNH 441

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BLAST of Carg25067 vs. Swiss-Prot
Match: sp|Q8GY11|WRK43_ARATH (Probable WRKY transcription factor 43 OS=Arabidopsis thaliana OX=3702 GN=WRKY43 PE=1 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.4e-26
Identity = 53/81 (65.43%), Postives = 68/81 (83.95%), Query Frame = 0


Query: 135 RKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHSNCRVKKRVERLS 194
           +K++ PRF F+T+SD D+LDDGY+WRKYGQK VKNSL+PRSYYRCT   C VKK+V+RLS
Sbjct: 11  KKMKNPRFSFRTKSDADILDDGYRWRKYGQKSVKNSLYPRSYYRCTQHMCNVKKQVQRLS 70

Query: 195 EDCRMVITTYEGRHNHSPCDD 216
           ++  +V TTYEG HNH PC++
Sbjct: 71  KETSIVETTYEGIHNH-PCEE 90

[Close]

BLAST of Carg25067 vs. Swiss-Prot
Match: sp|Q9FFS3|WRK24_ARATH (Probable WRKY transcription factor 24 OS=Arabidopsis thaliana OX=3702 GN=WRKY24 PE=2 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 5.2e-26
Identity = 53/85 (62.35%), Postives = 68/85 (80.00%), Query Frame = 0


Query: 130 KVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHSNCRVKKR 189
           ++K +R  + PR  F TRSD DVLDDGY+WRKYGQK VK++ HPRSYYRCT+  C VKK+
Sbjct: 74  ELKEKRSRKVPRIAFHTRSDDDVLDDGYRWRKYGQKSVKHNAHPRSYYRCTYHTCNVKKQ 133

Query: 190 VERLSEDCRMVITTYEGRHNHSPCD 215
           V+RL++D  +V+TTYEG HNH PC+
Sbjct: 134 VQRLAKDPNVVVTTYEGVHNH-PCE 157

[Close]

BLAST of Carg25067 vs. TrEMBL
Match: tr|A0A1U6ZIF2|A0A1U6ZIF2_9ROSI (probable WRKY transcription factor 12 OS=Juglans regia OX=51240 GN=WRKY12 PE=2 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 2.5e-81
Identity = 176/231 (76.19%), Postives = 188/231 (81.39%), Query Frame = 0


Query: 1   MEGSQRSELPNYQLQVSFLSNAPHPQPIHEMGFLHFEDHNQVLSFLAP----XXXXXXXX 60
           ME  QR   PNY+LQVSF +    PQ I EMGF+ FED NQVLSFLAP            
Sbjct: 1   MEEDQRGSAPNYELQVSFSTT---PQAIPEMGFVQFED-NQVLSFLAPSQSSQISQPLSG 60

Query: 61  XXXXXXXXXXXXXXXXXXXXXXXXXHAHLLTRPSWSNDQVGTLDPKPGGDENCTGSTSDG 120
            XXXXXXXXXXXXXXXXX       H  L+TR SW+NDQVGT+DPK   DENCTG+ SDG
Sbjct: 61  GXXXXXXXXXXXXXXXXXPTNMGFSHNDLVTRSSWNNDQVGTMDPKAVNDENCTGNASDG 120

Query: 121 TNSWWRNSNADKTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSY 180
            NSWWR+SN+DK+KVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSY
Sbjct: 121 NNSWWRSSNSDKSKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSY 180

Query: 181 YRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSDHEPFTSF 228
           YRCTH+NCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSS+HE FTSF
Sbjct: 181 YRCTHNNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSEHECFTSF 227

[Close]

BLAST of Carg25067 vs. TrEMBL
Match: tr|A0A1S3AVD7|A0A1S3AVD7_CUCME (probable WRKY transcription factor 12 OS=Cucumis melo OX=3656 GN=LOC103483097 PE=4 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 2.1e-80
Identity = 176/220 (80.00%), Postives = 192/220 (87.27%), Query Frame = 0


Query: 11  NYQLQVSF-LSNAPHPQPIHEMGFLHFEDHNQVLSFLAPXXXXXXXXXXXXXXXXXXXXX 70
           +Y++QVSF LSN PH   IHEMGF+  E+H+QVLSFLAP             XXXXXXXX
Sbjct: 16  DYEVQVSFHLSNDPH--HIHEMGFVQLEEHSQVLSFLAP-------------XXXXXXXX 75

Query: 71  XXXXXXXXXXXXHAHLLTR-PSWSNDQV-GTLDPKPGGDENCTGSTSDGTNSWWRNSNAD 130
           XXXXXXXXXXXXH+++L R PSW+N Q+ GTLDPKPG DENCT + +D TNSWWRN+NAD
Sbjct: 76  XXXXXXXXXXXXHSNILPRPPSWTNHQLPGTLDPKPGNDENCTATATDATNSWWRNTNAD 135

Query: 131 KTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHSNCRVK 190
           K+KVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHSNCRVK
Sbjct: 136 KSKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHSNCRVK 195

Query: 191 KRVERLSEDCRMVITTYEGRHNHSPCDDSNSSDHEPFTSF 228
           KRVERLSEDCRMVITTYEGRHNHSPCDDSNSS+HEPFTSF
Sbjct: 196 KRVERLSEDCRMVITTYEGRHNHSPCDDSNSSEHEPFTSF 220

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BLAST of Carg25067 vs. TrEMBL
Match: tr|A0A0A0L3J0|A0A0A0L3J0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G116700 PE=4 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 4.6e-80
Identity = 174/220 (79.09%), Postives = 191/220 (86.82%), Query Frame = 0


Query: 11  NYQLQVSF-LSNAPHPQPIHEMGFLHFEDHNQVLSFLAPXXXXXXXXXXXXXXXXXXXXX 70
           +Y++QVSF LSN PH   IHEMGF+  E+H+QVLSFLAP              XXXXXXX
Sbjct: 16  DYEVQVSFHLSNDPH--HIHEMGFVQLEEHSQVLSFLAP--------------XXXXXXX 75

Query: 71  XXXXXXXXXXXXHAHLLTR-PSWSNDQV-GTLDPKPGGDENCTGSTSDGTNSWWRNSNAD 130
           XXXXXXXXXXXXH+++L R PSW+N Q+ GTLDPKPG DENCT + +D TNSWWRN+NAD
Sbjct: 76  XXXXXXXXXXXXHSNILPRPPSWTNHQLPGTLDPKPGNDENCTATATDATNSWWRNTNAD 135

Query: 131 KTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHSNCRVK 190
           K+KVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHSNCRVK
Sbjct: 136 KSKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHSNCRVK 195

Query: 191 KRVERLSEDCRMVITTYEGRHNHSPCDDSNSSDHEPFTSF 228
           KRVERLSEDCRMVITTYEGRHNH+PCDDSNSS+HEPFTSF
Sbjct: 196 KRVERLSEDCRMVITTYEGRHNHTPCDDSNSSEHEPFTSF 219

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BLAST of Carg25067 vs. TrEMBL
Match: tr|A0A2P4K7B9|A0A2P4K7B9_QUESU (Putative wrky transcription factor 12 OS=Quercus suber OX=58331 GN=CFP56_57221 PE=4 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 3.3e-78
Identity = 181/224 (80.80%), Postives = 195/224 (87.05%), Query Frame = 0


Query: 10  PNYQLQVSFLSNAPHPQPIHEMGFLHFEDHNQVLSFLAP------XXXXXXXXXXXXXXX 69
           PNY+LQVSF +    PQ IH+MGF+ FED NQVLSFLAP      XXXXXXXXXXXXXXX
Sbjct: 8   PNYELQVSFSAT---PQAIHDMGFVQFED-NQVLSFLAPSQSSXXXXXXXXXXXXXXXXX 67

Query: 70  XXXXXXXXXXXXXXXXXXHAHLLTRPSWSNDQVGTLDPKPGGDENCTGSTSDGTNSWWRN 129
           XXXXXXXXXXXXX     H  L+TRPSW+NDQVGT+DPK   DE+ TG+ SDG NSWWR+
Sbjct: 68  XXXXXXXXXXXXXTMGFSHNDLVTRPSWNNDQVGTMDPKAVNDESGTGNASDGNNSWWRS 127

Query: 130 SNADKTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHSN 189
           S +DK+KVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTH+N
Sbjct: 128 STSDKSKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHNN 187

Query: 190 CRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSDHEPFTSF 228
           CRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSS+HE FTSF
Sbjct: 188 CRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSEHECFTSF 227

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BLAST of Carg25067 vs. TrEMBL
Match: tr|A0A1S3ZUB8|A0A1S3ZUB8_TOBAC (probable WRKY transcription factor 12 OS=Nicotiana tabacum OX=4097 GN=LOC107790562 PE=4 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 9.7e-78
Identity = 175/242 (72.31%), Postives = 195/242 (80.58%), Query Frame = 0


Query: 1   MEGSQRSELPNYQLQVSFLSNAPHPQPIHEMGFLHFEDHNQVLSFLAP------------ 60
           ME  +  EL NY LQVSF +   H   IH++GF+HF DHNQ++SFLAP            
Sbjct: 1   MERGEERELTNYDLQVSFSAPPSHANNIHDLGFVHFADHNQMMSFLAPPSSHSQLSSQLP 60

Query: 61  ---XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHAHLLTRPSWSNDQVGTLDPKPGG 120
               XXXXXXXXXXXXXXXXXXXXXXXXXXX     H  L+ RPSW+N+QV TLDPK   
Sbjct: 61  QPLQXXXXXXXXXXXXXXXXXXXXXXXXXXXALGFNHNELVNRPSWNNNQVETLDPKIVN 120

Query: 121 DENCTGSTSDGTNSWWRNSNADKTKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQK 180
           DENC+G+ S+G NSWW+NS++DK KVK+RRKLREPRFCFQTRSD+DVLDDGYKWRKYGQK
Sbjct: 121 DENCSGNASEGNNSWWKNSSSDKGKVKIRRKLREPRFCFQTRSDIDVLDDGYKWRKYGQK 180

Query: 181 VVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPCDDSNSSDHEPFT 228
           VVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSP DDSNSS+H+ FT
Sbjct: 181 VVKNSLHPRSYYRCTHSNCRVKKRVERLSEDCRMVITTYEGRHNHSPSDDSNSSEHDCFT 240

The following BLAST results are available for this feature:

BLAST of Carg25067 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022935668.1	8.8e-107	97.80	probable WRKY transcription factor 12 [Cucurbita moschata]	[more]
XP_023538960.1	4.4e-106	98.68	probable WRKY transcription factor 12 isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_022974266.1	7.4e-106	97.37	probable WRKY transcription factor 12 isoform X2 [Cucurbita maxima]	[more]
XP_023538959.1	4.5e-103	97.38	probable WRKY transcription factor 12 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022974265.1	7.7e-103	96.09	probable WRKY transcription factor 12 isoform X1 [Cucurbita maxima]	[more]

BLAST of Carg25067 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G44745.1	7.8e-49	88.00	WRKY family transcription factor	[more]
AT4G39410.1	8.9e-37	68.18	WRKY DNA-binding protein 13	[more]
AT2G46130.1	7.6e-28	65.43	WRKY DNA-binding protein 43	[more]
AT5G41570.1	2.9e-27	62.35	WRKY DNA-binding protein 24	[more]
AT1G69310.1	3.8e-27	69.74	WRKY DNA-binding protein 57	[more]

BLAST of Carg25067 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q93WY4\|WRK12_ARATH	1.4e-47	88.00	Probable WRKY transcription factor 12 OS=Arabidopsis thaliana OX=3702 GN=WRKY12 ...	[more]
sp\|Q9SVB7\|WRK13_ARATH	1.6e-35	68.18	Probable WRKY transcription factor 13 OS=Arabidopsis thaliana OX=3702 GN=WRKY13 ...	[more]
sp\|Q6B6R4\|WRK24_ORYSI	6.1e-27	48.39	WRKY transcription factor WRKY24 OS=Oryza sativa subsp. indica OX=39946 GN=WRKY2...	[more]
sp\|Q8GY11\|WRK43_ARATH	1.4e-26	65.43	Probable WRKY transcription factor 43 OS=Arabidopsis thaliana OX=3702 GN=WRKY43 ...	[more]
sp\|Q9FFS3\|WRK24_ARATH	5.2e-26	62.35	Probable WRKY transcription factor 24 OS=Arabidopsis thaliana OX=3702 GN=WRKY24 ...	[more]

BLAST of Carg25067 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1U6ZIF2\|A0A1U6ZIF2_9ROSI	2.5e-81	76.19	probable WRKY transcription factor 12 OS=Juglans regia OX=51240 GN=WRKY12 PE=2 S...	[more]
tr\|A0A1S3AVD7\|A0A1S3AVD7_CUCME	2.1e-80	80.00	probable WRKY transcription factor 12 OS=Cucumis melo OX=3656 GN=LOC103483097 PE...	[more]
tr\|A0A0A0L3J0\|A0A0A0L3J0_CUCSA	4.6e-80	79.09	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G116700 PE=4 SV=1	[more]
tr\|A0A2P4K7B9\|A0A2P4K7B9_QUESU	3.3e-78	80.80	Putative wrky transcription factor 12 OS=Quercus suber OX=58331 GN=CFP56_57221 P...	[more]
tr\|A0A1S3ZUB8\|A0A1S3ZUB8_TOBAC	9.7e-78	72.31	probable WRKY transcription factor 12 OS=Nicotiana tabacum OX=4097 GN=LOC1077905...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0043565	sequence-specific DNA binding
GO:0003700	transcription factor activity, sequence-specific DNA binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006355	regulation of transcription, DNA-templated

Vocabulary: **INTERPRO**
Term	Definition
IPR036576	WRKY_dom_sf
IPR003657	WRKY_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005667	transcription factor complex
molecular_function	GO:0043565	sequence-specific DNA binding
molecular_function	GO:0003700	transcription factor activity, sequence-specific DNA binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25067-RA	Carg25067-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003657	WRKY domain	SMART	SM00774	WRKY_cls	coord: 153..212 e-value: 6.5E-38 score: 142.0
IPR003657	WRKY domain	PFAM	PF03106	WRKY	coord: 154..210 e-value: 2.9E-25 score: 88.3
IPR003657	WRKY domain	PROSITE	PS50811	WRKY	coord: 148..210 score: 29.608
IPR036576	WRKY domain superfamily	GENE3D	G3DSA:2.20.25.80		coord: 139..213 e-value: 6.2E-32 score: 111.1
IPR036576	WRKY domain superfamily	SUPERFAMILY	SSF118290	WRKY DNA-binding domain	coord: 145..211
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 208..227
None	No IPR available	PANTHER	PTHR31221:SF17	WRKY TRANSCRIPTION FACTOR 12-RELATED	coord: 51..218
None	No IPR available	PANTHER	PTHR31221	FAMILY NOT NAMED	coord: 51..218

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg25067	Cla021067	Watermelon (97103) v1	carwmB1013
Carg25067	Csa3G116700	Cucumber (Chinese Long) v2	carcuB1025
Carg25067	MELO3C005937	Melon (DHL92) v3.5.1	carmeB0985
Carg25067	CSPI03G07040	Wild cucumber (PI 183967)	carcpiB1049
Carg25067	Cucsa.260060	Cucumber (Gy14) v1	carcgyB1129
Carg25067	Lsi05G022330	Bottle gourd (USVL1VR-Ls)	carlsiB926
Carg25067	Cp4.1LG08g00210	Cucurbita pepo (Zucchini)	carcpeB1410
Carg25067	CsGy3G007180	Cucumber (Gy14) v2	carcgybB0997
Carg25067	MELO3C005937.2	Melon (DHL92) v3.6.1	carmedB0995
Carg25067	CsaV3_3G007160	Cucumber (Chinese Long) v3	carcucB1055
Carg25067	Cla97C05G080000	Watermelon (97103) v2	carwmbB1070
Carg25067	Bhi01G000457	Wax gourd	carwgoB1188

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg25067	Cucurbita maxima (Rimu)	carcmaB1405
Carg25067	Cucurbita moschata (Rifu)	carcmoB1362
Carg25067	Watermelon (Charleston Gray)	carwcgB0988