Carg13978 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg13978
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	mediator of RNA polymerase II transcription subunit 21
Location	Cucurbita_argyrosperma_scaffold_097 : 130598 .. 134118 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTTACTCTCTCGTGCTCCGCCCGGTCACGTCGTCTCCCCCTCCCTTTCAGCCGTTCAGTGTCGCCACCGCTTCTGTCCGGTCCTTGTCGCCGCCGCCTCTCTCTGGTCAGTGCTGCCGCCGCAGCCTCCCTCAGGTTTGCACTGATTTTATACTCTTCTGATGTTACCGATATTCTCCCTTTCTTCATCTCTGCCTTTTTTCTCGCTTGAAAAACTCAAATTGGGAATTGTATAAGGAAAAAATGGAGTTGTTTTGATTTTTTGAAATGACAATAGACGAACTGCGGCGTGCTTTGCCTTTAGATTCGTTGAGTATGTCTACTGAGGCTTGATCGTCTGTGCATCTGTCTTGTTTCTGCGAGAATGAAATGTTCAGCGTAATTATTTCTTTGTGTATGAGAGAATCAGTAAGTTATTGTATTATCGAATTCTTTTTTAATCCCTAGCTTGGCGAAGCTATTTTGATTGACCAACAACTTTCAACCATACTTGTAGTTGTAGATTTGGAGACTATAGTTGGCAGATAACAATGAATCTTTTTTTAAAGTTGAAATTCTCCTCCCTCACATCCATCAATCACCCAACATACTTTTAATTTACTGGAAATAAAAGCCATCTATATGCTTTGTACTTCATGTAATTGCCATAAAAAGTTTTAGAAAATGCATAGAGTAAAATAGGAAGGGAACTAACGATTTTCCTATATAGTATAGATTATTCCGGTATTGGTACGGCACTGATACTTTTTTCGTTGGCATAATCCAACTTGGCTGCTCTTCCAAGGTTTTCGGTGAGCTCAGTTTTGTGAGAATTGGAATTGGTTTATCCATTAATGATAAACAGATCTAACATCTTACAATCACGTCATTACAATTTTGATTTCGATCCTCTAATACTGTTTCCTTATATTTGACGTGATTTCATCATTTACTTGAGCAACCAAACACAAGATCCAGATTAGAACCTGCGAACCTATTGACTCACTCCTAGTCTCTTCAATTGTAATAAATTAAATTGAGAAACTATGGATCGAGGTATTTCATTCAGTGTTTCTTTTATTGAATTGGTTGTTTTTCTTCCACAACGTTGTCTCTCAGCAATTAAAGTCGGATGTGAAGTCTTCTTACGAGTATAAACTTTCCTTACCAAGTTTTTGATGCTATTTCAGATTTTTTTTTTTTTTTTTGGATTATATTGGAAAAAATAGGTTGGACTCCCACTCAGTCAGTTCCTAAATGTGATATGTTGATTTTAATTGTCTTGATATGTTTATTGGTTCTCATCATTTTCTGGACAATTCTTCTGGGGGATAAGGTTCGAAATGGACATTATTTCCCAACTACAGGAACAAGTCAATACAATTGCTGCTTTAGCTTTCAATACTTTTGGGACATTGCAGAGAGATGCTCCTCCAGTTCGTTTATCTCCTAATTACCCCGAACCTCCGCCTCACGAACCTACAGAGGACAGTGCAAACTTCGCAGAGCAGCCCAAGCTAATGAGTGCCGCTTTGGTGAAAGCCGCTAAGCAGGTGTGATTTTTATTTTGCAGGATTGTGTTTTCCGTTGTTCATGCTAAGTGTGTAGTTCGTTGGTTCTTGTACTTTTGGGGTGGAAAATATGAATGTGATGCATATGCCTCTTGTTATTTACTTTTTCATGGGTCGAAACATCAAGAAAGTATTTTCTTCTTCCTTTTGGGGAGATGTTAGATTGCCATTTTTATTTTTTTTTTCCAAATGTCAGTATCCTAGATTGATTAACTTTACATGCTTTCTATCTACTGGATAAGTAATTGAGAAACAACTGTCTACTCGAAGCTCCTTTGTGATGAGATTGAAAGAGATGCATTTCATTCACCCTCAAATGTTCGTTTGTTAGATCTAGTTAATGCCAAGTCTGAACTTGTGTTGTCTGAGGATGAAACAGGACTGTGATTTCCTGCTTTACAGCCGCTCATAACTTCTATTGCGTTTTTTATGGGGTAGTAATATATCAAAGCAACGGATCTCCTGCTACTTGTTTGAGATGCATACTAATTCTAATGCTATGATTTATTGTATATCCATCAAAGGAAGCAAATTTGGTATTGGAAACTTCAATTTACTTTTGCCTTCCAACCTCAGATACTACTTGGGCAAATATGGTGTTTTAGATCAGCAATAAGCCAAAGTCTTACGGAAAACTTTTCTAACTATTCTTTGCTTTTAACTGTATATATGTTTTAGCCCCACTTTTAAGAGCTATTAAGCGTGTTCATATTTTTGGAATCTGCTATTCAACCCTGTATTTGACTAAAATCTGGATCTTTTTTTTATTTAGAAAATTTTGCTGAGAGATTTTGATTTTCGTAAGCAATGGAGTTGAAAGTAGCAATGAATTAAGGTTTTTTCTCTTCAAAAGTTTTTTCCATGGAATATGTAGAATTTATAAAATCGACGAGTCTGTCCACATTACGTTTGAGAAAATCCCAGTGTAGTTTTTGGGTAATAGAAATGTTTGGATGTTTTCCAGTTCTGGTAGCATTTTAGTGCCTTCTAGATGTGATTCTACAAGGAGCCAGATTGTTCCATTCCTTCTCTCATTTTCTTTTCCTTTTTTACTCTCTCCTCTCAAACTGGTATACTATTAGCTTTCTGTACAAAAGAAAGGGTTGAGACCATGCTTCATTTATATATAATCCAATTGAACATATGTACCGTTCTTGAAACTATTGTTGTTATTATCTTTTGGCACAGTTTGATGTATTAGTTGCAGCATTGCCTCTATCTGATGGAGGTGAAGAAGAGCAGTTGAAAAGGATTGCAGAACTACAGGTATAATAAGCCTTCTCATATTCTAATGTTGATATAAGACCAAGAATTCCTGTATTGTTTAGTTCTCATTACATTCGAAACTTGTTATCCAGGCCGAGAACGATGCTGTAGGTCAAGAACTTCAAAAGCAGCTAGAAGCAGCAGGTATTATCTCGTTTTCTTCATTAACCTGTGTATATTTACAACATGCCCAGTTTCAAATTATGGGCCCACCAAGAATGACTTCCTATTGGAAATTTTACTGTATGGTTTCGTTTGGGGTTTGACCAACTCGTCATAAGCGCGAAATTTCTCGTGTTCTCTCCGTTCTATTGAACATACTACTGATATTTGGCCACCGCTACACAATCTAACATTCTTAATGGAATTGGGAATTGTTTGTTGCAGAGAAGGAATTAAAACAGGTTCAGGAGCTTTTCAGCCAAGTTGCAGATAATTGCTTGAACTTGAAGAAAGCAGACTGAAAGATTTCAAGGTTATAATAGTAGATCAATCAAATGCATTCATTCCTTTCTTCCCAATTCAATGCAACTTAGAACAGGTGTGTTAATTTTCTGCGCCACTTTGTCCATTTTCTCATCGTTTTTTTAGCTCAACTCGGTAGAAGATTCATGATGCCTAACCCCAAATTGCCTCTCTTTAGCTTCTAGCGGATGTTCAAAGTTGTCAAAGTAATTGCAAGTTAGCAATATATTATGTATTTG

mRNA sequence

ATTTTACTCTCTCGTGCTCCGCCCGGTCACGTCGTCTCCCCCTCCCTTTCAGCCGTTCAGTGTCGCCACCGCTTCTGTCCGGTCCTTGTCGCCGCCGCCTCTCTCTGGTCAGTGCTGCCGCCGCAGCCTCCCTCAGGTTCGAAATGGACATTATTTCCCAACTACAGGAACAAGTCAATACAATTGCTGCTTTAGCTTTCAATACTTTTGGGACATTGCAGAGAGATGCTCCTCCAGTTCGTTTATCTCCTAATTACCCCGAACCTCCGCCTCACGAACCTACAGAGGACAGTGCAAACTTCGCAGAGCAGCCCAAGCTAATGAGTGCCGCTTTGGTGAAAGCCGCTAAGCAGTTTGATGTATTAGTTGCAGCATTGCCTCTATCTGATGGAGGTGAAGAAGAGCAGTTGAAAAGGATTGCAGAACTACAGGCCGAGAACGATGCTGTAGGTCAAGAACTTCAAAAGCAGCTAGAAGCAGCAGAGAAGGAATTAAAACAGGTTCAGGAGCTTTTCAGCCAAGTTGCAGATAATTGCTTGAACTTGAAGAAAGCAGACTGAAAGATTTCAAGGTTATAATAGTAGATCAATCAAATGCATTCATTCCTTTCTTCCCAATTCAATGCAACTTAGAACAGGTGTGTTAATTTTCTGCGCCACTTTGTCCATTTTCTCATCGTTTTTTTAGCTCAACTCGGTAGAAGATTCATGATGCCTAACCCCAAATTGCCTCTCTTTAGCTTCTAGCGGATGTTCAAAGTTGTCAAAGTAATTGCAAGTTAGCAATATATTATGTATTTG

Coding sequence (CDS)

ATGGACATTATTTCCCAACTACAGGAACAAGTCAATACAATTGCTGCTTTAGCTTTCAATACTTTTGGGACATTGCAGAGAGATGCTCCTCCAGTTCGTTTATCTCCTAATTACCCCGAACCTCCGCCTCACGAACCTACAGAGGACAGTGCAAACTTCGCAGAGCAGCCCAAGCTAATGAGTGCCGCTTTGGTGAAAGCCGCTAAGCAGTTTGATGTATTAGTTGCAGCATTGCCTCTATCTGATGGAGGTGAAGAAGAGCAGTTGAAAAGGATTGCAGAACTACAGGCCGAGAACGATGCTGTAGGTCAAGAACTTCAAAAGCAGCTAGAAGCAGCAGAGAAGGAATTAAAACAGGTTCAGGAGCTTTTCAGCCAAGTTGCAGATAATTGCTTGAACTTGAAGAAAGCAGACTGA

Protein sequence

MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLMSAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQVQELFSQVADNCLNLKKAD

Homology

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BLAST of Carg13978 vs. NCBI nr
Match: XP_022958635.1 (mediator of RNA polymerase II transcription subunit 21 [Cucurbita moschata] >XP_022958636.1 mediator of RNA polymerase II transcription subunit 21 [Cucurbita moschata] >XP_022996209.1 mediator of RNA polymerase II transcription subunit 21 [Cucurbita maxima] >XP_022996211.1 mediator of RNA polymerase II transcription subunit 21 [Cucurbita maxima])

HSP 1 Score: 258.8 bits (660), Expect = 1.0e-65
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60

Query: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120
           SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV
Sbjct: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

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BLAST of Carg13978 vs. NCBI nr
Match: XP_023532119.1 (mediator of RNA polymerase II transcription subunit 21 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 256.1 bits (653), Expect = 6.6e-65
Identity = 136/138 (98.55%), Postives = 137/138 (99.28%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPT+DSANF EQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTDDSANFVEQPKLM 60

Query: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120
           SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV
Sbjct: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

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BLAST of Carg13978 vs. NCBI nr
Match: XP_022153730.1 (mediator of RNA polymerase II transcription subunit 21 [Momordica charantia])

HSP 1 Score: 247.3 bits (630), Expect = 3.1e-62
Identity = 134/138 (97.10%), Postives = 134/138 (97.10%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLS NYPEPP  EPTEDSAN AEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSSNYPEPPSQEPTEDSANIAEQPKLM 60

Query: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120
           SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV
Sbjct: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

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BLAST of Carg13978 vs. NCBI nr
Match: XP_004149640.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Cucumis sativus] >KGN54019.1 hypothetical protein Csa_4G268030 [Cucumis sativus])

HSP 1 Score: 240.0 bits (611), Expect = 4.9e-60
Identity = 129/138 (93.48%), Postives = 131/138 (94.93%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPP  EPTED+A  AEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60

Query: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120
           SAALVKAAKQFD LVAALPLSDGGEEEQLKRIAELQAEND VGQELQKQL+AAEKEL QV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

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BLAST of Carg13978 vs. NCBI nr
Match: XP_008449895.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Cucumis melo])

HSP 1 Score: 239.6 bits (610), Expect = 6.4e-60
Identity = 129/138 (93.48%), Postives = 131/138 (94.93%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPP  EPTEDSA  AEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDSATIAEQPKLM 60

Query: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120
           SAALVKAAKQFD LVAALPLSDGGEEEQLKRIAELQAEND VGQELQKQL+AAE+EL QV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEQELVQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

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BLAST of Carg13978 vs. TAIR10
Match: AT4G04780.1 (mediator 21)

HSP 1 Score: 203.4 bits (516), Expect = 9.2e-53
Identity = 111/139 (79.86%), Postives = 119/139 (85.61%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEP-TEDSANFAEQPKL 60
           MDIISQLQEQVNTIAA+ FN FGTLQRDAPPV+LSPNYPEPP     T+D+  F EQPK 
Sbjct: 1   MDIISQLQEQVNTIAAITFNAFGTLQRDAPPVQLSPNYPEPPATTTVTDDATPFPEQPKQ 60

Query: 61  MSAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQ 120
           +SA LVKAAKQFD LVAALPLS+GGE  QLKRIAELQ END VGQELQKQLEAAEKELKQ
Sbjct: 61  LSAGLVKAAKQFDALVAALPLSEGGEGAQLKRIAELQVENDLVGQELQKQLEAAEKELKQ 120

Query: 121 VQELFSQVADNCLNLKKAD 139
           VQELF Q ADNCLN+KK +
Sbjct: 121 VQELFGQAADNCLNMKKPE 139

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BLAST of Carg13978 vs. Swiss-Prot
Match: sp|C0LU16|MED21_ARATH (Mediator of RNA polymerase II transcription subunit 21 OS=Arabidopsis thaliana OX=3702 GN=MED21 PE=1 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 1.7e-51
Identity = 111/139 (79.86%), Postives = 119/139 (85.61%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEP-TEDSANFAEQPKL 60
           MDIISQLQEQVNTIAA+ FN FGTLQRDAPPV+LSPNYPEPP     T+D+  F EQPK 
Sbjct: 1   MDIISQLQEQVNTIAAITFNAFGTLQRDAPPVQLSPNYPEPPATTTVTDDATPFPEQPKQ 60

Query: 61  MSAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQ 120
           +SA LVKAAKQFD LVAALPLS+GGE  QLKRIAELQ END VGQELQKQLEAAEKELKQ
Sbjct: 61  LSAGLVKAAKQFDALVAALPLSEGGEGAQLKRIAELQVENDLVGQELQKQLEAAEKELKQ 120

Query: 121 VQELFSQVADNCLNLKKAD 139
           VQELF Q ADNCLN+KK +
Sbjct: 121 VQELFGQAADNCLNMKKPE 139

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BLAST of Carg13978 vs. Swiss-Prot
Match: sp|Q7ZTI5|MED21_DANRE (Mediator of RNA polymerase II transcription subunit 21 OS=Danio rerio OX=7955 GN=med21 PE=2 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 5.2e-05
Identity = 38/137 (27.74%), Postives = 67/137 (48.91%), Query Frame = 0


Query: 2   DIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLS---PNYPEPPPHEPTEDSANFAEQPK 61
           D ++QLQ+ VN++A    N  G LQ+ APP   S       +  P  PTE+ A      +
Sbjct: 3   DRLTQLQDAVNSLADQFCNAIGVLQQCAPPASFSNIQTAINKDQPSNPTEEYA------Q 62

Query: 62  LMSAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELK 121
           L +A + + AK  DVL+ +LP  +     Q   + +L+ EN      L++ +   +  L+
Sbjct: 63  LFAALIARTAKDVDVLIDSLPSEESTAALQAASLRQLEEENQEAAARLEEVVYRGDALLE 122

Query: 122 QVQELFSQVADNCLNLK 136
           ++Q   + +A + L  +
Sbjct: 123 KIQTALADIAQSQLRTR 133

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BLAST of Carg13978 vs. Swiss-Prot
Match: sp|Q6BER6|MED21_CAEEL (Mediator of RNA polymerase II transcription subunit 21 OS=Caenorhabditis elegans OX=6239 GN=mdt-21 PE=1 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 1.2e-04
Identity = 36/129 (27.91%), Postives = 60/129 (46.51%), Query Frame = 0


Query: 2   DIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLMS 61
           D ++QLQ+ +N +A L  N  G LQ  APP        E       ED  N A    + +
Sbjct: 3   DRMTQLQDMINEMAGLMTNAIGVLQATAPPCEFGAISQE------LEDEPNCA----IFA 62

Query: 62  AALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQVQ 121
           A + KAAK  ++L+ + P+  G +E +++                +K ++  EK+ ++V 
Sbjct: 63  AGIAKAAKNIEILIDSFPIEAGNQEAEVE----------------EKMIKNDEKQREKVN 105

Query: 122 ELFSQVADN 131
           EL   V D+
Sbjct: 123 ELVGLVGDS 105

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BLAST of Carg13978 vs. Swiss-Prot
Match: sp|Q2TBU8|MED21_BOVIN (Mediator of RNA polymerase II transcription subunit 21 OS=Bos taurus OX=9913 GN=MED21 PE=2 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 4.4e-04
Identity = 37/137 (27.01%), Postives = 65/137 (47.45%), Query Frame = 0


Query: 2   DIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLS---PNYPEPPPHEPTEDSANFAEQPK 61
           D ++QLQ+ VN++A    N  G LQ+  PP   S       +  P  PTE+ A      +
Sbjct: 3   DRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFSNIQTAINKDQPANPTEEYA------Q 62

Query: 62  LMSAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELK 121
           L +A + + AK  DVL+ +LP  +     Q   + +L+ EN      L+  +   +  L+
Sbjct: 63  LFAALIARTAKDIDVLIDSLPSEESTAALQAASLYKLEEENHEAATSLEDVVYRGDMLLE 122

Query: 122 QVQELFSQVADNCLNLK 136
           ++Q   + +A + L  +
Sbjct: 123 KIQSALADIAQSQLKTR 133

[Close]

BLAST of Carg13978 vs. Swiss-Prot
Match: sp|Q61BU1|MED21_CAEBR (Mediator of RNA polymerase II transcription subunit 21 OS=Caenorhabditis briggsae OX=6238 GN=mdt-21 PE=3 SV=2)

HSP 1 Score: 44.7 bits (104), Expect = 9.9e-04
Identity = 38/131 (29.01%), Postives = 66/131 (50.38%), Query Frame = 0


Query: 2   DIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLMS 61
           D ++QLQ+ +N +A+L  N  G LQ  APP        +   HE   + A FA       
Sbjct: 3   DRMTQLQDMINEMASLMTNAIGVLQATAPPCEFDAISQD---HEEEPNCAIFA------- 62

Query: 62  AALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQ-- 121
           A++ K+AK  ++L+ + P+  G  E++   + +   +NDAV +E   +L+    E  Q  
Sbjct: 63  ASIAKSAKNIEILIDSFPIEAGNMEDE---VEQKMIQNDAVQREKVGELKELVGESTQLV 120

Query: 122 --VQELFSQVA 129
             VQ+  S+++
Sbjct: 123 SAVQKKLSEIS 120

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BLAST of Carg13978 vs. TrEMBL
Match: tr|A0A0A0KZ00|A0A0A0KZ00_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G268030 PE=4 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 3.2e-60
Identity = 129/138 (93.48%), Postives = 131/138 (94.93%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPP  EPTED+A  AEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60

Query: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120
           SAALVKAAKQFD LVAALPLSDGGEEEQLKRIAELQAEND VGQELQKQL+AAEKEL QV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

[Close]

BLAST of Carg13978 vs. TrEMBL
Match: tr|A0A1S3BN38|A0A1S3BN38_CUCME (mediator of RNA polymerase II transcription subunit 21 OS=Cucumis melo OX=3656 GN=LOC103491637 PE=4 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 4.2e-60
Identity = 129/138 (93.48%), Postives = 131/138 (94.93%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPP  EPTEDSA  AEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDSATIAEQPKLM 60

Query: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120
           SAALVKAAKQFD LVAALPLSDGGEEEQLKRIAELQAEND VGQELQKQL+AAE+EL QV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEQELVQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

[Close]

BLAST of Carg13978 vs. TrEMBL
Match: tr|K4B9R4|K4B9R4_SOLLC (Uncharacterized protein OS=Solanum lycopersicum OX=4081 GN=101256647 PE=4 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 1.8e-58
Identity = 126/138 (91.30%), Postives = 128/138 (92.75%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPP  PTEDSAN AEQPK M
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPANPTEDSANVAEQPKQM 60

Query: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120
           SAA VKAAKQFDVLVAALPLSDG EE QLKRIAELQAENDAVGQELQKQLEAAEKELKQV
Sbjct: 61  SAAFVKAAKQFDVLVAALPLSDGSEEAQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELF+Q  DNCLNLKK +
Sbjct: 121 QELFNQATDNCLNLKKPE 138

[Close]

BLAST of Carg13978 vs. TrEMBL
Match: tr|A0A1S4BT41|A0A1S4BT41_TOBAC (mediator of RNA polymerase II transcription subunit 21-like isoform X1 OS=Nicotiana tabacum OX=4097 GN=LOC107811605 PE=4 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 2.3e-58
Identity = 125/138 (90.58%), Postives = 128/138 (92.75%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPP  PTED+AN AEQPK M
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPANPTEDAANVAEQPKQM 60

Query: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120
           SAA VKAAKQFD LVAALPLSDGGEE QLKRIAELQAENDAVGQELQKQLEAAEKELKQV
Sbjct: 61  SAAFVKAAKQFDALVAALPLSDGGEEAQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELF+Q  DNCLNLKK +
Sbjct: 121 QELFNQATDNCLNLKKPE 138

[Close]

BLAST of Carg13978 vs. TrEMBL
Match: tr|A0A1J6IVT0|A0A1J6IVT0_NICAT (Mediator of rna polymerase ii transcription subunit 21 OS=Nicotiana attenuata OX=49451 GN=MED21 PE=4 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 2.3e-58
Identity = 125/138 (90.58%), Postives = 128/138 (92.75%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPP  PTED+AN AEQPK M
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPANPTEDAANVAEQPKQM 60

Query: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120
           SAA VKAAKQFD LVAALPLSDGGEE QLKRIAELQAENDAVGQELQKQLEAAEKELKQV
Sbjct: 61  SAAFVKAAKQFDALVAALPLSDGGEEAQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELF+Q  DNCLNLKK +
Sbjct: 121 QELFNQATDNCLNLKKPE 138

The following BLAST results are available for this feature:

BLAST of Carg13978 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022958635.1	1.0e-65	100.00	mediator of RNA polymerase II transcription subunit 21 [Cucurbita moschata] >XP_...	[more]
XP_023532119.1	6.6e-65	98.55	mediator of RNA polymerase II transcription subunit 21 [Cucurbita pepo subsp. pe...	[more]
XP_022153730.1	3.1e-62	97.10	mediator of RNA polymerase II transcription subunit 21 [Momordica charantia]	[more]
XP_004149640.1	4.9e-60	93.48	PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Cucumis sativ...	[more]
XP_008449895.1	6.4e-60	93.48	PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Cucumis melo]	[more]

BLAST of Carg13978 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G04780.1	9.2e-53	79.86	mediator 21	[more]

BLAST of Carg13978 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|C0LU16\|MED21_ARATH	1.7e-51	79.86	Mediator of RNA polymerase II transcription subunit 21 OS=Arabidopsis thaliana O...	[more]
sp\|Q7ZTI5\|MED21_DANRE	5.2e-05	27.74	Mediator of RNA polymerase II transcription subunit 21 OS=Danio rerio OX=7955 GN...	[more]
sp\|Q6BER6\|MED21_CAEEL	1.2e-04	27.91	Mediator of RNA polymerase II transcription subunit 21 OS=Caenorhabditis elegans...	[more]
sp\|Q2TBU8\|MED21_BOVIN	4.4e-04	27.01	Mediator of RNA polymerase II transcription subunit 21 OS=Bos taurus OX=9913 GN=...	[more]
sp\|Q61BU1\|MED21_CAEBR	9.9e-04	29.01	Mediator of RNA polymerase II transcription subunit 21 OS=Caenorhabditis briggsa...	[more]

BLAST of Carg13978 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KZ00\|A0A0A0KZ00_CUCSA	3.2e-60	93.48	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G268030 PE=4 SV=1	[more]
tr\|A0A1S3BN38\|A0A1S3BN38_CUCME	4.2e-60	93.48	mediator of RNA polymerase II transcription subunit 21 OS=Cucumis melo OX=3656 G...	[more]
tr\|K4B9R4\|K4B9R4_SOLLC	1.8e-58	91.30	Uncharacterized protein OS=Solanum lycopersicum OX=4081 GN=101256647 PE=4 SV=1	[more]
tr\|A0A1S4BT41\|A0A1S4BT41_TOBAC	2.3e-58	90.58	mediator of RNA polymerase II transcription subunit 21-like isoform X1 OS=Nicoti...	[more]
tr\|A0A1J6IVT0\|A0A1J6IVT0_NICAT	2.3e-58	90.58	Mediator of rna polymerase ii transcription subunit 21 OS=Nicotiana attenuata OX...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Cellular Component**
Term	Definition
GO:0016592	mediator complex

Vocabulary: **INTERPRO**
Term	Definition
IPR037212	Med7/Med21-like
IPR021384	Mediator_Med21

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0016592	mediator complex
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg13978-RA	Carg13978-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 85..130
None	No IPR available	GENE3D	G3DSA:1.20.58.470		coord: 1..130 e-value: 8.0E-20 score: 73.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 28..51
IPR021384	Mediator complex, subunit Med21	PFAM	PF11221	Med21	coord: 1..128 e-value: 2.6E-29 score: 102.2
IPR021384	Mediator complex, subunit Med21	PANTHER	PTHR13381	RNA POLYMERASE II HOLOENZYME COMPONENT SRB7	coord: 1..137
IPR037212	Mediator complex, subunit Med7/Med21-like	SUPERFAMILY	SSF140718	Mediator hinge subcomplex-like	coord: 1..126

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg13978	Csa4G268030	Cucumber (Chinese Long) v2	carcuB0535
Carg13978	MELO3C014729	Melon (DHL92) v3.5.1	carmeB0512
Carg13978	CSPI04G12500	Wild cucumber (PI 183967)	carcpiB0542
Carg13978	Cucsa.152690	Cucumber (Gy14) v1	carcgyB0580
Carg13978	CmaCh02G011630	Cucurbita maxima (Rimu)	carcmaB0716
Carg13978	CmoCh02G011970	Cucurbita moschata (Rifu)	carcmoB0694
Carg13978	Lsi10G009150	Bottle gourd (USVL1VR-Ls)	carlsiB458
Carg13978	Cp4.1LG05g05620	Cucurbita pepo (Zucchini)	carcpeB0718
Carg13978	MELO3C014729.2	Melon (DHL92) v3.6.1	carmedB0518
Carg13978	CsaV3_4G017560	Cucumber (Chinese Long) v3	carcucB0539
Carg13978	Cla97C02G050680	Watermelon (97103) v2	carwmbB0531
Carg13978	Bhi10G000403	Wax gourd	carwgoB0618

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg13978	Cucumber (Gy14) v2	carcgybB0511
Carg13978	Watermelon (Charleston Gray)	carwcgB0493
Carg13978	Watermelon (97103) v1	carwmB0505