Lsi10G009150 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi10G009150
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Mediator of RNA polymerase II transcription subunit 21
Location	chr10 : 13626756 .. 13631614 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGTCGTTGATCTTGCAAAACGATTCTGTGATGGAGCAGCACCCCGTATTATTAATGGATGCCTTAGGACCTTTACAAAGGACATCAAAGAAATTGATTCAACTCATGTCGGAAGCAAGAAGTCCGTGCATAACCCTGAACTTTTGAGGTGCCTCAGGACCTTTGTAAGGATATTGAAGAATTTGACTCAACTCATGTTGTAGCTCAAGTAAGTGATTTGGGGGTACGTTCATTAAACTTGTTGTTGTCATCAGCAGCTAACGTGAAGTGATTTGAAGAATCTCATACTTGCCGCAGATCACAACAATAATTTCTTTCCCTTTCGCCCATGCAGGCGTGGATTTCTGTTATATCATAGAGTTGAGTAATGAGGTGCCTGATGTGTGAGAGAATAAGTTCTCAAATTAATCTAGTACCGGGAGAGATGTTATTATACACATAATATTATCAATATAGTGAAGTTTTAGTAAAGGTGCGATGTGTCGTGGTCATGTTTGTCCAGTGTGCCCCTGGTTGCATGCCTCTAGCAAATTTTAGTTTTAATCCTTTCTACCCGAAGTTGTGTGTTAAGCCGTGTTGTTCCTCTTATATGCTTTGTAGCCTACAACACAACCTCCCAAATAGCGATGAATCAAAAGTGAGGGTCGATTTAGATTGATTTTCTAAGTGTTTAAATAATTGCAAAAAGAAGTGTTTATGAACACTTAGAAAGTCAATGCAAACGGGCCCTCATTTTAATTCATTATAAATATAATAAGTCTATTGCAGTCTATCGTTGATCGATAGTGAAATTTTTCTATATTGGTAAATAGTTTGATATTTTTTTTACTTATAATAATTTTCTTAATAATTACTCTTAAATTTATCTTAGGGGTGTTTGGCTCATCAACATGAGTTGGTAAAAATTAACAACTCAATGTTTGATGTTGGTGGAGTTGAGTTGGTTATTTTTACTAACTCACCTCAATTCGCTCTAACTCAATTCAATTCTTCCTCCTACCGATGTTTTCAATGACCCCACTCGTCGGCCACCACCGACGACCACCAATGCCATACTTCGACGACCAACTTCGATGACTACCTTTGTGCGGTCAACTTCGGGCTCCAGAAACCACCTTTTCAATTCCAGTGATAACCACTTCTGACGACCAACATTGACGTTCACCTCAAGCGACCAACTCCGACAAAGACCACCATTGATGATCAACTGCGATAACCACTTATAGCAGCCAACTTCAACGATCACCTCAGGCGATTAACTCCAACCACCAACTTTGTCGTTTATCTCCAACGACTAACCCTAACAACCAATTCAACCAATCATATACTAAAAGAAAAAAAAATCTGATTGTTGAATCTTATCTATATACAAAATGATATACCAATTCAACATCTAACCACCCCTTCGATTAATCATATTCTACTATTATCAATTGCATTTATTAGATCGAGTAGGTATACATTCGATGAGTCATGTCCCATCTCAAAGAATTGCACTTTGGCTACATGAGGAAAGGGTAGAAAATGAATTTATTAAAAATATATGGGCAAAAGAGAACAAAATATTCTCCTTCATAACTCTATTTATAAAAATTAAACATGTTATAAATTTGAAAATTTGAATTTAAATTATCTTAAAGTGGTAAGTTGTCGGGATGCTTTTACTCGGTAGATAAGATATTAAATTTTGTTCGGTTCAATCTATTGTGACTACCAATTTAATATTCTACAAGTTTTTTTACACCAAAATGTTGTAAAATAATTAGGCAAGATGCACCTAAGCTGACCCGAACCGTCATAAATATAAAAAAAAAAAATTAAATTTTGTTAACATAACAATTATTGGGATAAGACAATTTCAACCTCCTGTTAAAAAAATGTCGATTATTATTAAGCCCTTATTTTGTGATGTGGTGTTCTACAATTAAAAAGAATAGGAAATTTAAAATAAAAAAAAAAAAGAAAAGAAAAAGAAAAAAAGATCATGATTAAAAATTGGAGAAGTAAAAGGAAAAGGAGGACAAAAGGAACCCTAATAGCTCATCTGTTTCCTCTCCCCCTCACGTTCAGCCGGCCAGGCCCTCCCTTGTCTCCGGCAGCAGCGCGACGACCACGGACCCGCGATGAAAATCCTTGGAGATTAACAGCGGATCGCACTCCCACTCAGTCAGTTTCTGGAAGTGGTTTGCTGATTTAATTGCAGTGATATGTGTGTTTGCTGAATCATCTTCTGGACATTTCTTCTTAGGGATAAGGTTGAAGATGGACATAATTTCCCAATTGCAGGAACAAGTTAACGCAATTGCTGCTTTAGCTTTCAACACATTTGGGACATTACAAAGAGACGCTCCTCCAGTTCGTTTATCCCCTAATTACCCTGAACCACCGTCCCACGAACCTACAGAGGACAGTGCAAATATCGCAGAGCAGCCCAAGCTTATGAGTGCCGCTTTGGTGAAAGCTGCTAAACAGGTGTGAATTATTTATTCTGTGGGATTTAAGTGTTTGCTTGATTCTTAATACTTCGGGGTGGGAAATATGAATTTCTGATGCATGTGCCTCTGGTATTAACTTCTTCACGGGTTGAAACATCAAGAAAATTATTTGCCTTTTCCTTTTGGGGAAAATTTATGTTGCTATTTTTGTTTTCAAATGTCAGTATCCTAAGATTAATTTTATATGCATTCTGTCTACTGGATAAGTAACCGAGAATCTACTATCTACTTGAAGCACCCTTCTGTTGAGGTTGGAAGAGAGTAATATCAAGGCAGCAGATTTCCTGCTACTTGTTTGAGATACATACACATTCTAATGCTATGATTTATGATACAATCCATCAAAATGAGCAAATTTGGTATTGGAAACTTCAATCTATTTTTGCCATCCAACGTCTGATACTACTTAGGTAGCTATAGTGTTTTGTATCGGCAATAAAAGAAAGTACTGTGAATTTTTGTAGTTATTCTTTGCTTTTAAGTTTTAACTGCCTGTTATGTATATTTAGCCCCACTTTAAGGAGCTCTTATGTGTGTTGATATTTTAGAATTCACTATTCAAACCCTGTATTCGAGCAATTTTGCTGAGAGAATTTTGATTTTCATAAGTTTTCAGCAACGGAGTTGAATGTAGCAATGAATTAAGGTTTGTTCTTTCGAACAATTTTTTTAAGGACTATATAGAATTTATAAAATTAACTGAGTGTATCCACATTACATTTGGGAATCCCAGTGTGATAGTAAGGTAATAGAAACGTGTAAAAGTTTTCCAATTCTGGTGAACTTTTAATGTCTTCTAGATGTGTTTCTACAAGGAGCCAGATTTGTTCTGTACATTATCTCATTTTCTGTATTTTGTATGCTCACCTCTCGATTGGTATATTATTTATTAGCTTTCTTAGGAGTTGCAGGGAGTCGACTGAGGAGTTTCTCCTCCGTCCACCTTCTTCTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTCTGTGTGTGTGTTGGAGAAAATGATGTTTTTGTGGTAGGTTGGAGTGTGTGCTATTTTGTGGGGTTTGTGGGCCGAGAGAAACAATATGATCTTTAAGGGTAGAAATAATAGGATCTTCAACGATAGAAATAATAGAATCTTGGAGATTCTTTGTTTCTCTTTGGTGTCAATTTTTTGAAATTACTCGCTTAGTCACATTCACTTGATTAGAACCCCTTCTTATCGTTAGCTTCCCTTTTTGTGGGCATAACCTTTTTGCATGTCAGTGTATTCTTTCATCTTTTTCCAATAAAAGTCTAGTTTTTACTTCAACAACAAAATTTATTAGCATTTTGGACAAAAGATAGGGTTGGGACCTTGCTTCATATATATTCAATCCAATTGAACATGTGTACCTTTTTAAAAAAATTGTTGTTATTATTTTCTGGCACAGTTTGATGCATTGGTTGCAGCATTGCCTCTATCTGATGGAGGTGAAGAAGAGCAGTTGAAAAGGATTGCAGAACTACAGGTATGAATCTTCTCATATTCCGATGTTATAAGACCAAGAATTCTTGTGTTGTTTACTTCTCAGTACGTTGGAAACTCGTTATCCATTCCAGGCTGAGAACGATGCTGTAGGTCAAGAACTTCAAAAGCAGCTACAAGCAGCAGGTGTTCTCTCGTCTTCTTGCTAATCTTTATATTTTCAACATCCCTAGATGCAATTATGAGTCCATCATGACTTCGAATGCATGCCCAAAAATAAGTTCCTATTTTAGATTGTTACTTCTGAATCGTTGTCTGGCTTGCTTGCGACTTACAATGTTCAAATAATTTAAGCCCTAAATTTCTCATGTTCACTCCGTTCTATTGAGAATATACTGATATTTTGCCACAGCTGCACAGCTAACTATCTCATTGAAACAATCTGTTGCAGAGAAAGAATTAATACAAGTTCAGGAGCTGTTCAACCAAGTGGCAGATAATTGCTTGAACTTGAAGAAAGCAGCTTGAAAGAGTGGGGGAATTTTCCTATACTACCAATTTATGAAAGTTTTCCATATCTCCCCCATTCAATTTCTGCCACATGGCTCTTTGTTTTTTATATTAAATATATATGTAGAAATATACGTGGCCCTACAGTTTAGGGAAGAAATATATTTCTATCAATGTCTTATTTTAAAAAGAAATAAAAAACTGTTCTCTTCTATTTATTTATTTATGTGCTTATTACTGTTGGAGAAATTATTTTAAATGATAAAACTATAGAAATATTTTTAAATATAGCAAAATGGTACGTTTTCATATGAGGAAACTTTTTATTATTTTGCTATATTTTTAAATAAGGTTGTTATTTTGCCATATATGAAAACATCCCTTATTATT

mRNA sequence

ATGAAGGTCGTTGATCTTGCAAAACGATTCTGTGATGGAGCAGCACCCCGTATTATTAATGGATGCCTTAGGACCTTTACAAAGGACATCAAAGAAATTGATTCAACTCATGTCGGAAGCAAGAAGTCCGTGCATAACCCTGAACTTTTGAGGTGCCTCAGGACCTTTATCACAACAATAATTTCTTTCCCTTTCGCCCATGCAGGCGTGGATTTCTGTTATATCATAGAGTTGAGTAATGAGGTGCCTGATGTCCGGCCAGGCCCTCCCTTGTCTCCGGCAGCAGCGCGACGACCACGGACCCGCGATGAAAATCCTTGGAGATTAACAGCGGATCGCACTCCCACTCAGTCAGTTTCTGGAAGTGGGATAAGGTTGAAGATGGACATAATTTCCCAATTGCAGGAACAAGTTAACGCAATTGCTGCTTTAGCTTTCAACACATTTGGGACATTACAAAGAGACGCTCCTCCAGTTCGTTTATCCCCTAATTACCCTGAACCACCGTCCCACGAACCTACAGAGGACAGTGCAAATATCGCAGAGCAGCCCAAGCTTATGAGTGCCGCTTTGGTGAAAGCTGCTAAACAGTTTGATGCATTGGTTGCAGCATTGCCTCTATCTGATGGAGGTGAAGAAGAGCAGTTGAAAAGGATTGCAGAACTACAGGCTGAGAACGATGCTGTAGGTCAAGAACTTCAAAAGCAGCTACAAGCAGCAGAGAAAGAATTAATACAAGTTCAGGAGCTGTTCAACCAAGTGGCAGATAATTGCTTGAACTTGAAGAAAGCAGCTTGAAAGAGTGGGGGAATTTTCCTATACTACCAATTTATGAAAGTTTTCCATATCTCCCCCATTCAATTTCTGCCACATGGCTCTTTGTTTTTTATATTAAATATATATGTAGAAATATACGTGGCCCTACAGTTTAGGGAAGAAATATATTTCTATCAATGTCTTATTTTAAAAAGAAATAAAAAACTGTTCTCTTCTATTTATTTATTTATGTGCTTATTACTGTTGGAGAAATTATTTTAAATGATAAAACTATAGAAATATTTTTAAATATAGCAAAATGGTACGTTTTCATATGAGGAAACTTTTTATTATTTTGCTATATTTTTAAATAAGGTTGTTATTTTGCCATATATGAAAACATCCCTTATTATT

Coding sequence (CDS)

ATGAAGGTCGTTGATCTTGCAAAACGATTCTGTGATGGAGCAGCACCCCGTATTATTAATGGATGCCTTAGGACCTTTACAAAGGACATCAAAGAAATTGATTCAACTCATGTCGGAAGCAAGAAGTCCGTGCATAACCCTGAACTTTTGAGGTGCCTCAGGACCTTTATCACAACAATAATTTCTTTCCCTTTCGCCCATGCAGGCGTGGATTTCTGTTATATCATAGAGTTGAGTAATGAGGTGCCTGATGTCCGGCCAGGCCCTCCCTTGTCTCCGGCAGCAGCGCGACGACCACGGACCCGCGATGAAAATCCTTGGAGATTAACAGCGGATCGCACTCCCACTCAGTCAGTTTCTGGAAGTGGGATAAGGTTGAAGATGGACATAATTTCCCAATTGCAGGAACAAGTTAACGCAATTGCTGCTTTAGCTTTCAACACATTTGGGACATTACAAAGAGACGCTCCTCCAGTTCGTTTATCCCCTAATTACCCTGAACCACCGTCCCACGAACCTACAGAGGACAGTGCAAATATCGCAGAGCAGCCCAAGCTTATGAGTGCCGCTTTGGTGAAAGCTGCTAAACAGTTTGATGCATTGGTTGCAGCATTGCCTCTATCTGATGGAGGTGAAGAAGAGCAGTTGAAAAGGATTGCAGAACTACAGGCTGAGAACGATGCTGTAGGTCAAGAACTTCAAAAGCAGCTACAAGCAGCAGAGAAAGAATTAATACAAGTTCAGGAGCTGTTCAACCAAGTGGCAGATAATTGCTTGAACTTGAAGAAAGCAGCTTGA

Protein sequence

MKVVDLAKRFCDGAAPRIINGCLRTFTKDIKEIDSTHVGSKKSVHNPELLRCLRTFITTIISFPFAHAGVDFCYIIELSNEVPDVRPGPPLSPAAARRPRTRDENPWRLTADRTPTQSVSGSGIRLKMDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLMSAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQVQELFNQVADNCLNLKKAA

Homology

[Close]

BLAST of Lsi10G009150 vs. Swiss-Prot
Match: MED21_ARATH (Mediator of RNA polymerase II transcription subunit 21 OS=Arabidopsis thaliana GN=MED21 PE=1 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 1.7e-49
Identity = 108/137 (78.83%), Postives = 117/137 (85.40%), Query Frame = 1


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEP-TEDSANIAEQPKL 187
           MDIISQLQEQVN IAA+ FN FGTLQRDAPPV+LSPNYPEPP+    T+D+    EQPK 
Sbjct: 1   MDIISQLQEQVNTIAAITFNAFGTLQRDAPPVQLSPNYPEPPATTTVTDDATPFPEQPKQ 60

Query: 188 MSAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQ 247
           +SA LVKAAKQFDALVAALPLS+GGE  QLKRIAELQ END VGQELQKQL+AAEKEL Q
Sbjct: 61  LSAGLVKAAKQFDALVAALPLSEGGEGAQLKRIAELQVENDLVGQELQKQLEAAEKELKQ 120

Query: 248 VQELFNQVADNCLNLKK 264
           VQELF Q ADNCLN+KK
Sbjct: 121 VQELFGQAADNCLNMKK 137

[Close]

BLAST of Lsi10G009150 vs. TrEMBL
Match: A0A0A0KZ00_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G268030 PE=4 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 2.3e-61
Identity = 130/137 (94.89%), Postives = 133/137 (97.08%), Query Frame = 1


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM 187
           MDIISQLQEQVN IAALAFNTFGTLQRDAPPVRLSPNYPEPPS EPTED+A IAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60

Query: 188 SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQV 247
           SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAEND VGQELQKQLQAAEKEL+QV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120

Query: 248 QELFNQVADNCLNLKKA 265
           QELF+QVADNCLNLKKA
Sbjct: 121 QELFSQVADNCLNLKKA 137

[Close]

BLAST of Lsi10G009150 vs. TrEMBL
Match: A0A0V0HDL0_SOLCH (Putative mediator of RNA polymerase II transcription subunit 21-like OS=Solanum chacoense PE=4 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 4.6e-57
Identity = 123/136 (90.44%), Postives = 125/136 (91.91%), Query Frame = 1


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM 187
           MDIISQLQEQVN IAALAFNTFGTLQRDAPPVRLSPNYPEPP   PTEDS N+AEQPK M
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPANPTEDSTNVAEQPKQM 60

Query: 188 SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQV 247
           SAA VKAAKQFDALVAALPLSDGGEE QLKRIAELQAENDAVGQELQKQL+AAEKEL QV
Sbjct: 61  SAAFVKAAKQFDALVAALPLSDGGEEAQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 248 QELFNQVADNCLNLKK 264
           QELFNQ  DNCLNLKK
Sbjct: 121 QELFNQATDNCLNLKK 136

[Close]

BLAST of Lsi10G009150 vs. TrEMBL
Match: M1BD21_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400016459 PE=4 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 4.6e-57
Identity = 123/136 (90.44%), Postives = 125/136 (91.91%), Query Frame = 1


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM 187
           MDIISQLQEQVN IAALAFNTFGTLQRDAPPVRLSPNYPEPP   PTEDS N+AEQPK M
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPANPTEDSTNVAEQPKQM 60

Query: 188 SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQV 247
           SAA VKAAKQFDALVAALPLSDGGEE QLKRIAELQAENDAVGQELQKQL+AAEKEL QV
Sbjct: 61  SAAFVKAAKQFDALVAALPLSDGGEEAQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 248 QELFNQVADNCLNLKK 264
           QELFNQ  DNCLNLKK
Sbjct: 121 QELFNQATDNCLNLKK 136

[Close]

BLAST of Lsi10G009150 vs. TrEMBL
Match: K4B9R4_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 2.3e-56
Identity = 122/136 (89.71%), Postives = 124/136 (91.18%), Query Frame = 1


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM 187
           MDIISQLQEQVN IAALAFNTFGTLQRDAPPVRLSPNYPEPP   PTEDSAN+AEQPK M
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPANPTEDSANVAEQPKQM 60

Query: 188 SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQV 247
           SAA VKAAKQFD LVAALPLSDG EE QLKRIAELQAENDAVGQELQKQL+AAEKEL QV
Sbjct: 61  SAAFVKAAKQFDVLVAALPLSDGSEEAQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 248 QELFNQVADNCLNLKK 264
           QELFNQ  DNCLNLKK
Sbjct: 121 QELFNQATDNCLNLKK 136

[Close]

BLAST of Lsi10G009150 vs. TrEMBL
Match: A0A0D2VBU8_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_010G179800 PE=4 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.2e-54
Identity = 120/136 (88.24%), Postives = 128/136 (94.12%), Query Frame = 1


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM 187
           MDIISQLQEQVN+IA+L FNTFGTLQRDAPPVRLSPNYPEPP + PTEDSAN A+QPKLM
Sbjct: 1   MDIISQLQEQVNSIASLTFNTFGTLQRDAPPVRLSPNYPEPPPN-PTEDSANFADQPKLM 60

Query: 188 SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQV 247
           SAALVKAAKQFDALVAALP S+GGEE QL+RIAELQAENDAVGQELQKQL+AAEKEL QV
Sbjct: 61  SAALVKAAKQFDALVAALPPSEGGEEAQLRRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 248 QELFNQVADNCLNLKK 264
           QELF+Q ADNCLNLKK
Sbjct: 121 QELFSQAADNCLNLKK 135

[Close]

BLAST of Lsi10G009150 vs. TAIR10
Match: AT4G04780.1 (AT4G04780.1 mediator 21)

HSP 1 Score: 197.6 bits (501), Expect = 9.7e-51
Identity = 108/137 (78.83%), Postives = 117/137 (85.40%), Query Frame = 1


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEP-TEDSANIAEQPKL 187
           MDIISQLQEQVN IAA+ FN FGTLQRDAPPV+LSPNYPEPP+    T+D+    EQPK 
Sbjct: 1   MDIISQLQEQVNTIAAITFNAFGTLQRDAPPVQLSPNYPEPPATTTVTDDATPFPEQPKQ 60

Query: 188 MSAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQ 247
           +SA LVKAAKQFDALVAALPLS+GGE  QLKRIAELQ END VGQELQKQL+AAEKEL Q
Sbjct: 61  LSAGLVKAAKQFDALVAALPLSEGGEGAQLKRIAELQVENDLVGQELQKQLEAAEKELKQ 120

Query: 248 VQELFNQVADNCLNLKK 264
           VQELF Q ADNCLN+KK
Sbjct: 121 VQELFGQAADNCLNMKK 137

[Close]

BLAST of Lsi10G009150 vs. TAIR10
Match: AT4G26370.1 (AT4G26370.1 antitermination NusB domain-containing protein)

HSP 1 Score: 57.0 bits (136), Expect = 2.0e-08
Identity = 24/28 (85.71%), Postives = 26/28 (92.86%), Query Frame = 1


Query: 2   KVVDLAKRFCDGAAPRIINGCLRTFTKD 30
           + VDLAKRFCDG+APRIINGCLRTF KD
Sbjct: 256 EAVDLAKRFCDGSAPRIINGCLRTFVKD 283

[Close]

BLAST of Lsi10G009150 vs. NCBI nr
Match: gi|449463842|ref|XP_004149640.1| (PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Cucumis sativus])

HSP 1 Score: 243.8 bits (621), Expect = 3.4e-61
Identity = 130/137 (94.89%), Postives = 133/137 (97.08%), Query Frame = 1


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM 187
           MDIISQLQEQVN IAALAFNTFGTLQRDAPPVRLSPNYPEPPS EPTED+A IAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60

Query: 188 SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQV 247
           SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAEND VGQELQKQLQAAEKEL+QV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120

Query: 248 QELFNQVADNCLNLKKA 265
           QELF+QVADNCLNLKKA
Sbjct: 121 QELFSQVADNCLNLKKA 137

[Close]

BLAST of Lsi10G009150 vs. NCBI nr
Match: gi|659097951|ref|XP_008449895.1| (PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Cucumis melo])

HSP 1 Score: 243.4 bits (620), Expect = 4.4e-61
Identity = 130/137 (94.89%), Postives = 133/137 (97.08%), Query Frame = 1


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM 187
           MDIISQLQEQVN IAALAFNTFGTLQRDAPPVRLSPNYPEPPS EPTEDSA IAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDSATIAEQPKLM 60

Query: 188 SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQV 247
           SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAEND VGQELQKQLQAAE+EL+QV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEQELVQV 120

Query: 248 QELFNQVADNCLNLKKA 265
           QELF+QVADNCLNLKKA
Sbjct: 121 QELFSQVADNCLNLKKA 137

[Close]

BLAST of Lsi10G009150 vs. NCBI nr
Match: gi|698553696|ref|XP_009770008.1| (PREDICTED: mediator of RNA polymerase II transcription subunit 21-like isoform X1 [Nicotiana sylvestris])

HSP 1 Score: 229.9 bits (585), Expect = 5.0e-57
Identity = 123/136 (90.44%), Postives = 126/136 (92.65%), Query Frame = 1


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM 187
           MDIISQLQEQVN IAALAFNTFGTLQRDAPPVRLSPNYPEPP   PTED+AN+AEQPK M
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPANPTEDAANVAEQPKQM 60

Query: 188 SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQV 247
           SAA VKAAKQFDALVAALPLSDGGEE QLKRIAELQAENDAVGQELQKQL+AAEKEL QV
Sbjct: 61  SAAFVKAAKQFDALVAALPLSDGGEEAQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 248 QELFNQVADNCLNLKK 264
           QELFNQ  DNCLNLKK
Sbjct: 121 QELFNQATDNCLNLKK 136

[Close]

BLAST of Lsi10G009150 vs. NCBI nr
Match: gi|565362742|ref|XP_006348104.1| (PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Solanum tuberosum])

HSP 1 Score: 229.6 bits (584), Expect = 6.5e-57
Identity = 123/136 (90.44%), Postives = 125/136 (91.91%), Query Frame = 1


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM 187
           MDIISQLQEQVN IAALAFNTFGTLQRDAPPVRLSPNYPEPP   PTEDS N+AEQPK M
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPANPTEDSTNVAEQPKQM 60

Query: 188 SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQV 247
           SAA VKAAKQFDALVAALPLSDGGEE QLKRIAELQAENDAVGQELQKQL+AAEKEL QV
Sbjct: 61  SAAFVKAAKQFDALVAALPLSDGGEEAQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 248 QELFNQVADNCLNLKK 264
           QELFNQ  DNCLNLKK
Sbjct: 121 QELFNQATDNCLNLKK 136

[Close]

BLAST of Lsi10G009150 vs. NCBI nr
Match: gi|970005966|ref|XP_015063828.1| (PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Solanum pennellii])

HSP 1 Score: 228.0 bits (580), Expect = 1.9e-56
Identity = 122/136 (89.71%), Postives = 124/136 (91.18%), Query Frame = 1


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM 187
           MDIISQLQEQVN IAALAFNTFGTLQRDAPPVRLSPNYPEPP   PTEDS N+AEQPK M
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPANPTEDSTNVAEQPKQM 60

Query: 188 SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQV 247
           SAA VKAAKQFD LVAALPLSDGGEE QLKRIAELQAENDAVGQELQKQL+AAEKEL QV
Sbjct: 61  SAAFVKAAKQFDVLVAALPLSDGGEEAQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 248 QELFNQVADNCLNLKK 264
           QELFNQ  DNCLNLKK
Sbjct: 121 QELFNQATDNCLNLKK 136

The following BLAST results are available for this feature:

BLAST of Lsi10G009150 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MED21_ARATH	1.7e-49	78.83	Mediator of RNA polymerase II transcription subunit 21 OS=Arabidopsis thaliana G...	[more]

BLAST of Lsi10G009150 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KZ00_CUCSA	2.3e-61	94.89	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G268030 PE=4 SV=1	[more]
A0A0V0HDL0_SOLCH	4.6e-57	90.44	Putative mediator of RNA polymerase II transcription subunit 21-like OS=Solanum ...	[more]
M1BD21_SOLTU	4.6e-57	90.44	Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400016459 PE=4 SV=1	[more]
K4B9R4_SOLLC	2.3e-56	89.71	Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1	[more]
A0A0D2VBU8_GOSRA	1.2e-54	88.24	Uncharacterized protein OS=Gossypium raimondii GN=B456_010G179800 PE=4 SV=1	[more]

BLAST of Lsi10G009150 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G04780.1	9.7e-51	78.83	mediator 21	[more]
AT4G26370.1	2.0e-08	85.71	antitermination NusB domain-containing protein	[more]

BLAST of Lsi10G009150 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449463842\|ref\|XP_004149640.1\|	3.4e-61	94.89	PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Cucumis sativ...	[more]
gi\|659097951\|ref\|XP_008449895.1\|	4.4e-61	94.89	PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Cucumis melo]	[more]
gi\|698553696\|ref\|XP_009770008.1\|	5.0e-57	90.44	PREDICTED: mediator of RNA polymerase II transcription subunit 21-like isoform X...	[more]
gi\|565362742\|ref\|XP_006348104.1\|	6.5e-57	90.44	PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Solanum tuber...	[more]
gi\|970005966\|ref\|XP_015063828.1\|	1.9e-56	89.71	PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Solanum penne...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003723	RNA binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006355	regulation of transcription, DNA-templated

Vocabulary: **INTERPRO**
Term	Definition
IPR021384	Mediator_Med21
IPR006027	NusB_RsmB_TIM44

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050832	defense response to fungus
biological_process	GO:0006357	regulation of transcription from RNA polymerase II promoter
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0008150	biological_process
cellular_component	GO:0016592	mediator complex
cellular_component	GO:0043078	polar nucleus
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0001104	RNA polymerase II transcription cofactor activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G009150.1	Lsi10G009150.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006027	NusB/RsmB/TIM44	GENE3D	G3DSA:1.10.940.10		coord: 3..32 score: 9.
IPR021384	Mediator complex, subunit Med21	PFAM	PF11221	Med21	coord: 128..255 score: 1.7
None	No IPR available	unknown	Coil	Coil	coord: 212..257 scor
None	No IPR available	PANTHER	PTHR13381	RNA POLYMERASE II HOLOENZYME COMPONENT SRB7	coord: 128..263 score: 1.3
None	No IPR available	PANTHER	PTHR13381:SF0	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 21	coord: 128..263 score: 1.3
None	No IPR available	unknown	SSF140718	Mediator hinge subcomplex-like	coord: 128..254 score: 7.85

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi10G009150	Csa4G268030	Cucumber (Chinese Long) v2	culsiB246
Lsi10G009150	CSPI04G12500	Wild cucumber (PI 183967)	cpilsiB245
Lsi10G009150	Cucsa.152690	Cucumber (Gy14) v1	cgylsiB245
Lsi10G009150	CmaCh02G011630	Cucurbita maxima (Rimu)	cmalsiB540
Lsi10G009150	CmoCh02G011970	Cucurbita moschata (Rifu)	cmolsiB525
Lsi10G009150	Cla003123	Watermelon (97103) v1	lsiwmB078
Lsi10G009150	MELO3C014729	Melon (DHL92) v3.5.1	lsimeB072
Lsi10G009150	ClCG02G024420	Watermelon (Charleston Gray)	lsiwcgB054
Lsi10G009150	Cla97C02G050680	Watermelon (97103) v2	lsiwmbB052
Lsi10G009150	Cp4.1LG05g05620	Cucurbita pepo (Zucchini)	cpelsiB598
Lsi10G009150	CsGy4G011750	Cucumber (Gy14) v2	cgyblsiB235
Lsi10G009150	Carg13978	Silver-seed gourd	carlsiB458
Lsi10G009150	CsaV3_4G017560	Cucumber (Chinese Long) v3	cuclsiB271

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi10G009150	Cucurbita maxima (Rimu)	cmalsiB500
Lsi10G009150	Cucurbita moschata (Rifu)	cmolsiB484
Lsi10G009150	Cucurbita pepo (Zucchini)	cpelsiB227
Lsi10G009150	Melon (DHL92) v3.6.1	lsimedB074
Lsi10G009150	Wax gourd	lsiwgoB079