CSPI01G07480 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI01G07480
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	E3 ubiquitin-protein ligase RING1-like
Location	Chr1 : 4748739 .. 4750312 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAACACAGCAAAGAACAACGTTGAAAAACAACACAGAGAGGAAAATAGAGAGGAGAGGAATGAACAGACTATTGGAAGAAGAAGCAGTTGATGAGATTATTTCATTTGCCTCCGACTTCCCTTCCCATGAGGATGAAGCCGTAGATCTTCTCCTGGATTTCATCACAAACGAGCATACATCAAGTACTTACATTTTTCTTTTAAAATGTTAATCCATGCTACATAGTTAAATTTATCTTGCTTTTTAGTTATTGGATCGGATGATTTAAAATAATATTAGAGCATGTTGGTTTAAGAGATCGTGCGCTCAAACCCAATCTTATTTTTTTCGACTTTTAGATGAATAGATGATTTAAGGCGGATTGTCCAAAATTGAGATGGCTAGAAATTTTGGTGAATAATATATGTTTTGACAAATATTCTTATAATTGAAGAGAAACAAATTAGTTTGTGCATTTGTATTATCAAATATTTTTCAATTCTTTGTTGATTGGGTGATTTTTCTTTTTCTTCTTTTTTTGCTAGATGGAGCTCTAGCAATAGATATGAATTTAGTTCATTGGGCAATTAACCGTTTACGTGTTGCCGATGCTGTCGTCGATGTTACCAATAGTGTCAGTTCAACACAACAAGTAATTTTCATTGATTTTTTTGGAACGATGGAATGGTTTAAGTCCAAACTTAATAATCAACAAGATATAAAATAGATTTTATGACAACTCACAAATAAATAGTTGAGTTCCGACTTTCGTTTTCTAAAAGTGAGAGATTGTTTGGTTCACCAAATGAAGTTAATAAATCTAAGTTAATCTATCCCATTTAATTAGTTTGTAAAATTGTAATATGGAATTTCTAATAAAGGTGAGTTTCAATAGTGTTTAATATTAAAATTGAATTGTTTACCCAAACTCAATAATTGAGCCAAACAACTCTATACAACCATAGTAATTATAATTGATAACGGTTTTAAAAATAATAAATAAGTTTTTTTTTTTCAATCCATAATAATTTTTATGATATTATTAAAGTATATTTAACATTTAAAAGATTTACAAACATTGCGCATTTTAGTTTTTAAAATTACACTAAGAATTCATAAAATTTCAAAATTTATAGTAATTTTAAAAAAAATTAAAAAATGATATACTAAATTTTCTAATTACCTTTATTTTTCTCGGAAACTAATATGCAGAACGCCTATTTGGAAAGCGATATTCTTGAAACGAGGGAAGGTGTAGTAGACGTTACTGGTTCTAATGACTTATTGGATGCTGACGATGAAGAGATGTGGATGGCGCACGTGAGACAACTTTCTCTAAACGATCTGAGCCTTCATGAAGGCTTTCCATTCCGGGCTTCAAAATCGACGATTCAGGCACTACCAGATGTTTTTACTGCGAAGGACTTTTCTTCTTCTTGGGTGTGTGGTGTGTGTTTGAATGATGAGATTGAGGCAAACGATGGGGCGAAACAGCTGCCCTGTAAACACATATTTCATACACATTGTATTATTTCTTGGTTGGAATTGTGCAATTCTTGTCCACTTTGTCGATACCAATTGCCCACATAG

mRNA sequence

ATGGAAACACAGCAAAGAACAACGTTGAAAAACAACACAGAGAGGAAAATAGAGAGGAGAGGAATGAACAGACTATTGGAAGAAGAAGCAGTTGATGAGATTATTTCATTTGCCTCCGACTTCCCTTCCCATGAGGATGAAGCCGTAGATCTTCTCCTGGATTTCATCACAAACGAGCATACATCAAATGGAGCTCTAGCAATAGATATGAATTTAGTTCATTGGGCAATTAACCGTTTACGTGTTGCCGATGCTGTCGTCGATGTTACCAATAGTGTCAGTTCAACACAACAAAACGCCTATTTGGAAAGCGATATTCTTGAAACGAGGGAAGGTGTAGTAGACGTTACTGGTTCTAATGACTTATTGGATGCTGACGATGAAGAGATGTGGATGGCGCACGTGAGACAACTTTCTCTAAACGATCTGAGCCTTCATGAAGGCTTTCCATTCCGGGCTTCAAAATCGACGATTCAGGCACTACCAGATGTTTTTACTGCGAAGGACTTTTCTTCTTCTTGGGTGTGTGGTGTGTGTTTGAATGATGAGATTGAGGCAAACGATGGGGCGAAACAGCTGCCCTGTAAACACATATTTCATACACATTGTATTATTTCTTGGTTGGAATTGTGCAATTCTTGTCCACTTTGTCGATACCAATTGCCCACATAG

Coding sequence (CDS)

ATGGAAACACAGCAAAGAACAACGTTGAAAAACAACACAGAGAGGAAAATAGAGAGGAGAGGAATGAACAGACTATTGGAAGAAGAAGCAGTTGATGAGATTATTTCATTTGCCTCCGACTTCCCTTCCCATGAGGATGAAGCCGTAGATCTTCTCCTGGATTTCATCACAAACGAGCATACATCAAATGGAGCTCTAGCAATAGATATGAATTTAGTTCATTGGGCAATTAACCGTTTACGTGTTGCCGATGCTGTCGTCGATGTTACCAATAGTGTCAGTTCAACACAACAAAACGCCTATTTGGAAAGCGATATTCTTGAAACGAGGGAAGGTGTAGTAGACGTTACTGGTTCTAATGACTTATTGGATGCTGACGATGAAGAGATGTGGATGGCGCACGTGAGACAACTTTCTCTAAACGATCTGAGCCTTCATGAAGGCTTTCCATTCCGGGCTTCAAAATCGACGATTCAGGCACTACCAGATGTTTTTACTGCGAAGGACTTTTCTTCTTCTTGGGTGTGTGGTGTGTGTTTGAATGATGAGATTGAGGCAAACGATGGGGCGAAACAGCTGCCCTGTAAACACATATTTCATACACATTGTATTATTTCTTGGTTGGAATTGTGCAATTCTTGTCCACTTTGTCGATACCAATTGCCCACATAG

Homology

[Close]

BLAST of CSPI01G07480 vs. Swiss-Prot
Match: RNG1L_ARATH (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.1e-17
Identity = 45/91 (49.45%), Postives = 59/91 (64.84%), Query Frame = 1


Query: 135 VRQLSLNDLSLHEGFPFRASKSTIQALPDVFTAKDFSSSWV--CGVCLNDEIEANDGAKQ 194
           ++QL+ ND + +   P  ASKS I ALP V   KD   S +  C VC+ DE E     KQ
Sbjct: 175 IQQLAENDPNRYGTPP--ASKSAIDALPTVKVTKDMLKSEMNQCAVCM-DEFEDGSDVKQ 234

Query: 195 LPCKHIFHTHCIISWLELCNSCPLCRYQLPT 224
           +PCKH+FH  C++ WLEL NSCP+CR++LPT
Sbjct: 235 MPCKHVFHQDCLLPWLELHNSCPVCRFELPT 262

[Close]

BLAST of CSPI01G07480 vs. Swiss-Prot
Match: RING1_GOSHI (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 8.1e-16
Identity = 43/91 (47.25%), Postives = 62/91 (68.13%), Query Frame = 1


Query: 135 VRQLSLNDLSLHEGFPFRASKSTIQALPDV-FTAKDFSSSW-VCGVCLNDEIEANDGAKQ 194
           ++QL+ ND + +   P  ASKS I+ALP V  T  + +S +  C VC+ D+ E    AKQ
Sbjct: 183 IQQLAENDPNRYGTPP--ASKSAIEALPLVNITKSNLNSEFNQCAVCM-DDFEEGTEAKQ 242

Query: 195 LPCKHIFHTHCIISWLELCNSCPLCRYQLPT 224
           +PCKH++H  C++ WLEL NSCP+CR++LPT
Sbjct: 243 MPCKHLYHKDCLLPWLELHNSCPVCRHELPT 270

[Close]

BLAST of CSPI01G07480 vs. Swiss-Prot
Match: RN181_DANRE (E3 ubiquitin-protein ligase RNF181 OS=Danio rerio GN=rnf181 PE=2 SV=2)

HSP 1 Score: 78.6 bits (192), Expect = 9.9e-14
Identity = 49/135 (36.30%), Postives = 67/135 (49.63%), Query Frame = 1


Query: 90  TNSVSSTQQNAYLESDILETREGVVDVTGSNDLLDADDEEMWMAHVRQLSLNDLSLHEGF 149
           TN     +QNA LE       +G+   +GS DL D D                       
Sbjct: 13  TNPEEQYRQNALLEL-ARSLMQGLDIDSGSFDLSDWDQR--------------------L 72

Query: 150 PFRASKSTIQALPDVFTAKDFSSSWV-CGVCLNDEIEANDGAKQLPCKHIFHTHCIISWL 209
           P  A+K+ +Q+LP V  + + +   V C VCL  E E  +  +++PCKH+FHT CI+ WL
Sbjct: 73  PPPAAKAVVQSLPVVIISPEQADKGVKCPVCLL-EFEEQESVREMPCKHLFHTGCILPWL 125

Query: 210 ELCNSCPLCRYQLPT 224
              NSCPLCR +LPT
Sbjct: 133 NKTNSCPLCRLELPT 125

[Close]

BLAST of CSPI01G07480 vs. Swiss-Prot
Match: RN181_XENLA (E3 ubiquitin-protein ligase RNF181 OS=Xenopus laevis GN=rnf181 PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.7e-13
Identity = 35/75 (46.67%), Postives = 49/75 (65.33%), Query Frame = 1


Query: 150 PFRASKSTIQALPDV-FTAKDFSSSWVCGVCLNDEIEANDGAKQLPCKHIFHTHCIISWL 209
           P  ASK  +++LP V  T +   ++  C VCL  E E  +  +QLPC+H+FH+ CI+ WL
Sbjct: 52  PPPASKKVVESLPKVTVTPEQADAALKCPVCLL-EFEEGETVRQLPCEHLFHSACILPWL 111

Query: 210 ELCNSCPLCRYQLPT 224
              NSCPLCR++LPT
Sbjct: 112 GKTNSCPLCRHELPT 125

[Close]

BLAST of CSPI01G07480 vs. Swiss-Prot
Match: RN181_XENTR (E3 ubiquitin-protein ligase RNF181 OS=Xenopus tropicalis GN=rnf181 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 6.4e-13
Identity = 34/75 (45.33%), Postives = 49/75 (65.33%), Query Frame = 1


Query: 150 PFRASKSTIQALPDV-FTAKDFSSSWVCGVCLNDEIEANDGAKQLPCKHIFHTHCIISWL 209
           P  A+K  +++LP V  T +   ++  C VCL  E E  +  +QLPC+H+FH+ CI+ WL
Sbjct: 52  PPPAAKKVVESLPKVTVTPEQADAALKCPVCLL-EFEEGETVRQLPCEHLFHSSCILPWL 111

Query: 210 ELCNSCPLCRYQLPT 224
              NSCPLCR++LPT
Sbjct: 112 GKTNSCPLCRHELPT 125

[Close]

BLAST of CSPI01G07480 vs. TrEMBL
Match: A0A0A0LQL0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G043080 PE=4 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 1.3e-121
Identity = 219/223 (98.21%), Postives = 220/223 (98.65%), Query Frame = 1


Query: 1   METQQRTTLKNNTERKIERRGMNRLLEEEAVDEIISFASDFPSHEDEAVDLLLDFITNEH 60
           METQQRTTLKNNTERKIERRGMNRLLEEEAVDEIISFASDFPSHED+AVDLLLDFITNEH
Sbjct: 1   METQQRTTLKNNTERKIERRGMNRLLEEEAVDEIISFASDFPSHEDDAVDLLLDFITNEH 60

Query: 61  TSNGALAIDMNLVHWAINRLRVADAVVDVTNSVSSTQQNAYLESDILETREGVVDVTGSN 120
           TSNGALAIDMNLVHWAIN LR ADAVVDVTNSVSSTQQNAYLESDILETREGVVDVT SN
Sbjct: 61  TSNGALAIDMNLVHWAINHLRAADAVVDVTNSVSSTQQNAYLESDILETREGVVDVTDSN 120

Query: 121 DLLDADDEEMWMAHVRQLSLNDLSLHEGFPFRASKSTIQALPDVFTAKDFSSSWVCGVCL 180
           DLLDADDEEMWMAHVRQLSLNDLSLHEGFPFRASKSTIQALPDVFTAKDFSSSWVCGVCL
Sbjct: 121 DLLDADDEEMWMAHVRQLSLNDLSLHEGFPFRASKSTIQALPDVFTAKDFSSSWVCGVCL 180

Query: 181 NDEIEANDGAKQLPCKHIFHTHCIISWLELCNSCPLCRYQLPT 224
           NDEIEANDGAKQLPCKHIFHTHCIISWLELCNSCPLCRYQLPT
Sbjct: 181 NDEIEANDGAKQLPCKHIFHTHCIISWLELCNSCPLCRYQLPT 223

[Close]

BLAST of CSPI01G07480 vs. TrEMBL
Match: A0A022PND4_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a008418mg PE=4 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.5e-16
Identity = 44/91 (48.35%), Postives = 61/91 (67.03%), Query Frame = 1


Query: 135 VRQLSLNDLSLHEGFPFRASKSTIQALPDVFTAKDF--SSSWVCGVCLNDEIEANDGAKQ 194
           ++QL+ ND + +   P  ASKS ++ LPD+   ++   S S  C VC  D  E N+ AKQ
Sbjct: 187 IQQLAENDPNRYGTPP--ASKSVVEGLPDITITQEMLTSDSSQCAVC-KDSFELNEAAKQ 246

Query: 195 LPCKHIFHTHCIISWLELCNSCPLCRYQLPT 224
           +PCKH++H  CI+ WLEL NSCP+CRY+LPT
Sbjct: 247 IPCKHLYHKDCIVPWLELHNSCPVCRYELPT 274

[Close]

BLAST of CSPI01G07480 vs. TrEMBL
Match: A0A022Q503_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a006235mg PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 4.3e-16
Identity = 45/91 (49.45%), Postives = 60/91 (65.93%), Query Frame = 1


Query: 135 VRQLSLNDLSLHEGFPFRASKSTIQALPDVFTAKDF--SSSWVCGVCLNDEIEANDGAKQ 194
           ++QL+ ND + +   P  ASKS ++ LPD+    +   S S  C VC  D  E N+ AKQ
Sbjct: 257 IQQLAENDPNRYGTPP--ASKSVVEGLPDIKITAEMLASDSSQCAVC-KDSFELNEEAKQ 316

Query: 195 LPCKHIFHTHCIISWLELCNSCPLCRYQLPT 224
           +PCKHI+H  CI+ WLEL NSCP+CRY+LPT
Sbjct: 317 IPCKHIYHKDCILPWLELHNSCPVCRYELPT 344

[Close]

BLAST of CSPI01G07480 vs. TrEMBL
Match: A0A0K9QXJ9_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_135340 PE=4 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 5.6e-16
Identity = 45/91 (49.45%), Postives = 59/91 (64.84%), Query Frame = 1


Query: 135 VRQLSLNDLSLHEGFPFRASKSTIQALPDVFTAKDF--SSSWVCGVCLNDEIEANDGAKQ 194
           ++QL+ ND + H   P  ASK  I  LP+V    D   S S  C VC+N E E +   KQ
Sbjct: 173 IQQLAENDPNRHGTPP--ASKEAIDDLPNVVITADVVNSDSNSCAVCMN-EFEVDSSVKQ 232

Query: 195 LPCKHIFHTHCIISWLELCNSCPLCRYQLPT 224
           +PCKH+FH+ C+I WL+L NSCP+CRY+LPT
Sbjct: 233 MPCKHLFHSECLIPWLQLHNSCPVCRYELPT 260

[Close]

BLAST of CSPI01G07480 vs. TrEMBL
Match: V4LVM7_EUTSA (Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10021059mg PE=4 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 9.6e-16
Identity = 45/91 (49.45%), Postives = 59/91 (64.84%), Query Frame = 1


Query: 135 VRQLSLNDLSLHEGFPFRASKSTIQALPDVFTAKDFSSSWV--CGVCLNDEIEANDGAKQ 194
           ++QL+ ND + +   P  ASKS I ALP V   KD   S +  C VC+ DE E     KQ
Sbjct: 180 IQQLAENDPNRYGTPP--ASKSAIDALPTVKVTKDMLKSEMNQCAVCM-DEFEDGSDVKQ 239

Query: 195 LPCKHIFHTHCIISWLELCNSCPLCRYQLPT 224
           +PCKH+FH  C++ WL+L NSCP+CRY+LPT
Sbjct: 240 MPCKHVFHQDCLLPWLQLHNSCPVCRYELPT 267

[Close]

BLAST of CSPI01G07480 vs. TAIR10
Match: AT3G19950.1 (AT3G19950.1 RING/U-box superfamily protein)

HSP 1 Score: 91.7 bits (226), Expect = 6.3e-19
Identity = 45/91 (49.45%), Postives = 59/91 (64.84%), Query Frame = 1


Query: 135 VRQLSLNDLSLHEGFPFRASKSTIQALPDVFTAKDFSSSWV--CGVCLNDEIEANDGAKQ 194
           ++QL+ ND + +   P  ASKS I ALP V   KD   S +  C VC+ DE E     KQ
Sbjct: 175 IQQLAENDPNRYGTPP--ASKSAIDALPTVKVTKDMLKSEMNQCAVCM-DEFEDGSDVKQ 234

Query: 195 LPCKHIFHTHCIISWLELCNSCPLCRYQLPT 224
           +PCKH+FH  C++ WLEL NSCP+CR++LPT
Sbjct: 235 MPCKHVFHQDCLLPWLELHNSCPVCRFELPT 262

[Close]

BLAST of CSPI01G07480 vs. TAIR10
Match: AT5G15820.1 (AT5G15820.1 RING/U-box superfamily protein)

HSP 1 Score: 80.5 bits (197), Expect = 1.5e-15
Identity = 50/142 (35.21%), Postives = 78/142 (54.93%), Query Frame = 1


Query: 84  DAVVDVTNSVSSTQQNAYLESDILETREGVVDVTGSNDLLDADDEEMWMAHVRQLSLNDL 143
           + V++   S+ S      L  ++  +    +DV    D +D      + A + Q+  N+ 
Sbjct: 199 EVVMEFAASIYSDAWEILLYDNMTNSAPMDLDVEVWLDSVDGYAPMDYNAIIGQMFDNET 258

Query: 144 SLHEGFPFRASKSTIQALPDV-FTAKDFSS-SWVCGVCLNDEIEANDGAKQLPCKHIFHT 203
            + +G P  ASKS +  LPDV  T ++ SS S VC +C  DE+   +  K+LPCKH +H 
Sbjct: 259 GI-KGTP-PASKSVVDGLPDVELTIEELSSVSIVCAIC-KDEVVFKEKVKRLPCKHYYHG 318

Query: 204 HCIISWLELCNSCPLCRYQLPT 224
            CII WL + N+CP+CR++LPT
Sbjct: 319 ECIIPWLGIRNTCPVCRHELPT 337

[Close]

BLAST of CSPI01G07480 vs. TAIR10
Match: AT3G13430.1 (AT3G13430.1 RING/U-box superfamily protein)

HSP 1 Score: 79.0 bits (193), Expect = 4.3e-15
Identity = 41/89 (46.07%), Postives = 57/89 (64.04%), Query Frame = 1


Query: 135 VRQLSLNDLSLHEGFPFRASKSTIQALPDVFTAKDFSSSWVCGVCLNDEIEANDGAKQLP 194
           +++L+ NDL+   G P  A+K  ++AL  V   K   S   C VCL D+ E    AK++P
Sbjct: 188 LQRLAENDLNNRYGTP-PATKEAVEALAMV---KIEDSLLQCSVCL-DDFEIGMEAKEMP 247

Query: 195 CKHIFHTHCIISWLELCNSCPLCRYQLPT 224
           CKH FH+ C++ WLEL +SCP+CRY LPT
Sbjct: 248 CKHKFHSDCLLPWLELHSSCPVCRYLLPT 271

[Close]

BLAST of CSPI01G07480 vs. TAIR10
Match: AT4G26400.2 (AT4G26400.2 RING/U-box superfamily protein)

HSP 1 Score: 78.2 bits (191), Expect = 7.3e-15
Identity = 38/88 (43.18%), Postives = 50/88 (56.82%), Query Frame = 1


Query: 135 VRQLSLNDLSLHEGFPFRASKSTIQALPDVFTAKDFSSSWVCGVCLNDEIEANDGAKQLP 194
           +  L+ ND   H   P R  K  +  LP V      S S  C +CL D+ +    AK++P
Sbjct: 206 LEHLADNDSIRHGSLPAR--KEVVDNLPTV----KISESLQCSICL-DDFDKGSEAKEMP 265

Query: 195 CKHIFHTHCIISWLELCNSCPLCRYQLP 223
           CKH FH  CI+ WLEL +SCP+CRY+LP
Sbjct: 266 CKHKFHIRCIVPWLELHSSCPVCRYELP 286

[Close]

BLAST of CSPI01G07480 vs. TAIR10
Match: AT5G56340.1 (AT5G56340.1 RING/U-box superfamily protein)

HSP 1 Score: 76.3 bits (186), Expect = 2.8e-14
Identity = 38/89 (42.70%), Postives = 55/89 (61.80%), Query Frame = 1


Query: 135 VRQLSLNDLSLHEGFPFRASKSTIQALPDVFTAKDFSSSWVCGVCLNDEIEANDGAKQLP 194
           ++ L+ ND +  +G P  A K  ++ALP V   +       C VCL D+ E    AK++P
Sbjct: 224 LQHLAENDPN-RQGTP-PARKEAVEALPTVKIMEPLQ----CSVCL-DDFEKGTEAKEMP 283

Query: 195 CKHIFHTHCIISWLELCNSCPLCRYQLPT 224
           CKH FH  CI+ WLEL +SCP+CR++LP+
Sbjct: 284 CKHKFHVRCIVPWLELHSSCPVCRFELPS 305

[Close]

BLAST of CSPI01G07480 vs. NCBI nr
Match: gi|700209109|gb|KGN64205.1| (hypothetical protein Csa_1G043080 [Cucumis sativus])

HSP 1 Score: 443.7 bits (1140), Expect = 1.9e-121
Identity = 219/223 (98.21%), Postives = 220/223 (98.65%), Query Frame = 1


Query: 1   METQQRTTLKNNTERKIERRGMNRLLEEEAVDEIISFASDFPSHEDEAVDLLLDFITNEH 60
           METQQRTTLKNNTERKIERRGMNRLLEEEAVDEIISFASDFPSHED+AVDLLLDFITNEH
Sbjct: 1   METQQRTTLKNNTERKIERRGMNRLLEEEAVDEIISFASDFPSHEDDAVDLLLDFITNEH 60

Query: 61  TSNGALAIDMNLVHWAINRLRVADAVVDVTNSVSSTQQNAYLESDILETREGVVDVTGSN 120
           TSNGALAIDMNLVHWAIN LR ADAVVDVTNSVSSTQQNAYLESDILETREGVVDVT SN
Sbjct: 61  TSNGALAIDMNLVHWAINHLRAADAVVDVTNSVSSTQQNAYLESDILETREGVVDVTDSN 120

Query: 121 DLLDADDEEMWMAHVRQLSLNDLSLHEGFPFRASKSTIQALPDVFTAKDFSSSWVCGVCL 180
           DLLDADDEEMWMAHVRQLSLNDLSLHEGFPFRASKSTIQALPDVFTAKDFSSSWVCGVCL
Sbjct: 121 DLLDADDEEMWMAHVRQLSLNDLSLHEGFPFRASKSTIQALPDVFTAKDFSSSWVCGVCL 180

Query: 181 NDEIEANDGAKQLPCKHIFHTHCIISWLELCNSCPLCRYQLPT 224
           NDEIEANDGAKQLPCKHIFHTHCIISWLELCNSCPLCRYQLPT
Sbjct: 181 NDEIEANDGAKQLPCKHIFHTHCIISWLELCNSCPLCRYQLPT 223

[Close]

BLAST of CSPI01G07480 vs. NCBI nr
Match: gi|659068755|ref|XP_008446033.1| (PREDICTED: ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucumis melo])

HSP 1 Score: 312.8 bits (800), Expect = 5.0e-82
Identity = 162/225 (72.00%), Postives = 179/225 (79.56%), Query Frame = 1


Query: 1   METQQRTTLKNNTERKIERRGMNRLLEEEAVDEIISFASDFPSHEDEAVDLLLDFITNEH 60
           ME QQ  TL+ NT+RKIE RGMNRLLEEEAVDEI+SFASDFPSHEDEAVDLLLD IT E 
Sbjct: 1   MEAQQIITLRTNTKRKIESRGMNRLLEEEAVDEILSFASDFPSHEDEAVDLLLDVITTEL 60

Query: 61  TSNGALAIDMNLVHWAINRLRVADAVVDVTNSVSSTQQNAYLESDILETREGVVDVTGSN 120
           TSN A  IDM+LVHWA+  LR A+       S  ST Q+A  ESDILETRE +VD+T SN
Sbjct: 61  TSNRARVIDMDLVHWAVTLLRAAN-----NTSADSTPQDANFESDILETRESIVDLTDSN 120

Query: 121 --DLLDADDEEMWMAHVRQLSLNDLSLHEGFPFRASKSTIQALPDVFTAKDFSSSWVCGV 180
             DLLD DD ++WM HVRQLSL+DL  H  FPF ASKS IQALPDVF   DFSSSW C V
Sbjct: 121 VTDLLDDDDVDIWM-HVRQLSLDDLIRHASFPFGASKSAIQALPDVFPTDDFSSSWACAV 180

Query: 181 CLNDEIEANDGAKQLPCKHIFHTHCIISWLELCNSCPLCRYQLPT 224
           C++DEI+A DGAKQLPC+HIFHTHCIISWLEL NSCPLCRY+LPT
Sbjct: 181 CMDDEIDAKDGAKQLPCEHIFHTHCIISWLELHNSCPLCRYKLPT 219

[Close]

BLAST of CSPI01G07480 vs. NCBI nr
Match: gi|727633764|ref|XP_010487953.1| (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Camelina sativa])

HSP 1 Score: 95.9 bits (237), Expect = 9.5e-17
Identity = 47/91 (51.65%), Postives = 60/91 (65.93%), Query Frame = 1


Query: 135 VRQLSLNDLSLHEGFPFRASKSTIQALPDVFTAKDFSSSWV--CGVCLNDEIEANDGAKQ 194
           ++QL+ ND + +   P  ASKS I ALP V   KD   S +  C VC+ DE E   G KQ
Sbjct: 174 IQQLAENDPNRYGTPP--ASKSAIDALPTVKVTKDMLKSEMNQCAVCM-DEFEDGSGVKQ 233

Query: 195 LPCKHIFHTHCIISWLELCNSCPLCRYQLPT 224
           +PCKH+FH  C++ WLEL NSCP+CRY+LPT
Sbjct: 234 MPCKHVFHQDCLLPWLELHNSCPVCRYELPT 261

[Close]

BLAST of CSPI01G07480 vs. NCBI nr
Match: gi|727414981|ref|XP_010508254.1| (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Camelina sativa])

HSP 1 Score: 95.9 bits (237), Expect = 9.5e-17
Identity = 47/91 (51.65%), Postives = 60/91 (65.93%), Query Frame = 1


Query: 135 VRQLSLNDLSLHEGFPFRASKSTIQALPDVFTAKDFSSSWV--CGVCLNDEIEANDGAKQ 194
           ++QL+ ND + +   P  ASKS I ALP V   KD   S +  C VC+ DE E   G KQ
Sbjct: 178 IQQLAENDPNRYGTPP--ASKSAIDALPTVKVTKDMLKSEMNQCAVCM-DEFEDGSGVKQ 237

Query: 195 LPCKHIFHTHCIISWLELCNSCPLCRYQLPT 224
           +PCKH+FH  C++ WLEL NSCP+CRY+LPT
Sbjct: 238 MPCKHVFHQDCLLPWLELHNSCPVCRYELPT 265

[Close]

BLAST of CSPI01G07480 vs. NCBI nr
Match: gi|727586242|ref|XP_010466163.1| (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Camelina sativa])

HSP 1 Score: 95.9 bits (237), Expect = 9.5e-17
Identity = 47/91 (51.65%), Postives = 60/91 (65.93%), Query Frame = 1


Query: 135 VRQLSLNDLSLHEGFPFRASKSTIQALPDVFTAKDFSSSWV--CGVCLNDEIEANDGAKQ 194
           ++QL+ ND + +   P  ASKS I ALP V   KD   S +  C VC+ DE E   G KQ
Sbjct: 178 IQQLAENDPNRYGTPP--ASKSAIDALPTVKVTKDMLKSEMNQCAVCM-DEFEDGSGVKQ 237

Query: 195 LPCKHIFHTHCIISWLELCNSCPLCRYQLPT 224
           +PCKH+FH  C++ WLEL NSCP+CRY+LPT
Sbjct: 238 MPCKHVFHQDCLLPWLELHNSCPVCRYELPT 265

The following BLAST results are available for this feature:

BLAST of CSPI01G07480 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RNG1L_ARATH	1.1e-17	49.45	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1...	[more]
RING1_GOSHI	8.1e-16	47.25	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1	[more]
RN181_DANRE	9.9e-14	36.30	E3 ubiquitin-protein ligase RNF181 OS=Danio rerio GN=rnf181 PE=2 SV=2	[more]
RN181_XENLA	3.7e-13	46.67	E3 ubiquitin-protein ligase RNF181 OS=Xenopus laevis GN=rnf181 PE=2 SV=1	[more]
RN181_XENTR	6.4e-13	45.33	E3 ubiquitin-protein ligase RNF181 OS=Xenopus tropicalis GN=rnf181 PE=2 SV=1	[more]

BLAST of CSPI01G07480 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LQL0_CUCSA	1.3e-121	98.21	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G043080 PE=4 SV=1	[more]
A0A022PND4_ERYGU	1.5e-16	48.35	Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a008418mg PE=4 SV=1	[more]
A0A022Q503_ERYGU	4.3e-16	49.45	Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a006235mg PE=4 SV=1	[more]
A0A0K9QXJ9_SPIOL	5.6e-16	49.45	Uncharacterized protein OS=Spinacia oleracea GN=SOVF_135340 PE=4 SV=1	[more]
V4LVM7_EUTSA	9.6e-16	49.45	Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10021059mg PE=4 SV=1	[more]

BLAST of CSPI01G07480 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G19950.1	6.3e-19	49.45	RING/U-box superfamily protein	[more]
AT5G15820.1	1.5e-15	35.21	RING/U-box superfamily protein	[more]
AT3G13430.1	4.3e-15	46.07	RING/U-box superfamily protein	[more]
AT4G26400.2	7.3e-15	43.18	RING/U-box superfamily protein	[more]
AT5G56340.1	2.8e-14	42.70	RING/U-box superfamily protein	[more]

BLAST of CSPI01G07480 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700209109\|gb\|KGN64205.1\|	1.9e-121	98.21	hypothetical protein Csa_1G043080 [Cucumis sativus]	[more]
gi\|659068755\|ref\|XP_008446033.1\|	5.0e-82	72.00	PREDICTED: ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucumis melo]	[more]
gi\|727633764\|ref\|XP_010487953.1\|	9.5e-17	51.65	PREDICTED: E3 ubiquitin-protein ligase RING1-like [Camelina sativa]	[more]
gi\|727414981\|ref\|XP_010508254.1\|	9.5e-17	51.65	PREDICTED: E3 ubiquitin-protein ligase RING1-like [Camelina sativa]	[more]
gi\|727586242\|ref\|XP_010466163.1\|	9.5e-17	51.65	PREDICTED: E3 ubiquitin-protein ligase RING1-like [Camelina sativa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001841	Znf_RING
IPR013083	Znf_RING/FYVE/PHD

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding
GO:0008270	zinc ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0008152	metabolic process
biological_process	GO:0006144	purine nucleobase metabolic process
biological_process	GO:0019083	viral transcription
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0031379	RNA-directed RNA polymerase complex
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008568	microtubule-severing ATPase activity
molecular_function	GO:0003968	RNA-directed RNA polymerase activity
molecular_function	GO:0016874	ligase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G07480.1	CSPI01G07480.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 176..218 score: 3.3
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 176..217 score: 4.
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 176..218 score: 11
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 175..219 score: 9.1
None	No IPR available	PANTHER	PTHR22763	RING ZINC FINGER PROTEIN	coord: 153..223 score: 7.4
None	No IPR available	PANTHER	PTHR22763:SF5	E3 UBIQUITIN-PROTEIN LIGASE CIP8	coord: 153..223 score: 7.4
None	No IPR available	unknown	SSF57850	RING/U-box	coord: 175..221 score: 5.74

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI01G07480	Csa1G043080	Cucumber (Chinese Long) v2	cpicuB000
CSPI01G07480	MELO3C002162	Melon (DHL92) v3.5.1	cpimeB024
CSPI01G07480	Lsi02G002680	Bottle gourd (USVL1VR-Ls)	cpilsiB021
CSPI01G07480	CsaV3_1G007450	Cucumber (Chinese Long) v3	cpicucB001
CSPI01G07480	CmaCh18G000960	Cucurbita maxima (Rimu)	cmacpiB394
CSPI01G07480	CmaCh13G009640	Cucurbita maxima (Rimu)	cmacpiB218
CSPI01G07480	CmoCh13G009990	Cucurbita moschata (Rifu)	cmocpiB204
CSPI01G07480	Cp4.1LG20g01760	Cucurbita pepo (Zucchini)	cpecpiB505
CSPI01G07480	CsGy1G007350	Cucumber (Gy14) v2	cgybcpiB001

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI01G07480	Watermelon (97103) v2	cpiwmbB010
CSPI01G07480	Wax gourd	cpiwgoB028
CSPI01G07480	Cucurbita pepo (Zucchini)	cpecpiB025
CSPI01G07480	Cucumber (Gy14) v1	cgycpiB160
CSPI01G07480	Cucurbita moschata (Rifu)	cmocpiB383
CSPI01G07480	Watermelon (Charleston Gray)	cpiwcgB024
CSPI01G07480	Watermelon (97103) v1	cpiwmB042
CSPI01G07480	Melon (DHL92) v3.6.1	cpimedB023
CSPI01G07480	Silver-seed gourd	carcpiB0418
CSPI01G07480	Silver-seed gourd	carcpiB0531