CmaCh17G006110 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh17G006110
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	rRNA N-glycosidase
Location	Cma_Chr17: 5202342 .. 5203169 (-)
RNA-Seq Expression	CmaCh17G006110
Synteny	CmaCh17G006110

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATGTTGTGTCGGTGTTTTGTTTGGTAATTCTGGCCATCTTCCATGGAAGTTCAAGTTGTGTTGCGGGTGACGTGAGCTTCAACCTGTTGGGTTCGAATTCCAAATCTTATAGCAACTTCATCAAAGCTCTAAGGAGTGCTCTTCCATCCAAGGACAAAGTATACAATATATCACTATTGCTTGGCTCGGCAACAGGCGCAAGTCGCTACACACAAGTGAAACTCTCCAATTATGACGGGAAAGCCATCACGGTGGCAATAGATGTAACAAACGTTTACATCATGGGGTATCTCGTGAACTCAACTTCCTACTTCTTCAACGAATCTGATGCCCAATTGGCTTCTAAATATGTGTTCAAAGATAGCACCCGTATTACACTTCCATACTCCGGTAATTACGAAAAGCTTCAAAATGCGGCAGGCAAAAAAAGAGAAAAAATTCCCCTTGGATTCCCAGCTTTGGACAGTGCAATTACCACCTTGTTTCATTACGACTCCACGGCTGCTGCTGCGGCATTTATCGTGGTGATTCAGTGCACGGCTGAGGCTTCAAGATACAAATATATCGAGGCCCAAATGATTAAGAGAATTTCCAAAGACGATGTCCCAAGTCCGGCGATTATCAGTTTAGAAAACAGCTGGTCTGCTCTCTCCAAACAAATTCAATTGGCTCAAACAAATAAAGGAGTTTTTAAAGCTCCGGTTTTGATTACAGACGATCAAGGCCAACGAGTTGAAATAACCAATGTCAGTTCCAACGTTGTAACCAAAAACATCCAGTTGCTACTAAACTCACAAAATATTGCAGCTTTGAGCTACAATTAG

mRNA sequence

ATGAATGTTGTGTCGGTGTTTTGTTTGGTAATTCTGGCCATCTTCCATGGAAGTTCAAGTTGTGTTGCGGGTGACGTGAGCTTCAACCTGTTGGGTTCGAATTCCAAATCTTATAGCAACTTCATCAAAGCTCTAAGGAGTGCTCTTCCATCCAAGGACAAAGTATACAATATATCACTATTGCTTGGCTCGGCAACAGGCGCAAGTCGCTACACACAAGTGAAACTCTCCAATTATGACGGGAAAGCCATCACGGTGGCAATAGATGTAACAAACGTTTACATCATGGGGTATCTCGTGAACTCAACTTCCTACTTCTTCAACGAATCTGATGCCCAATTGGCTTCTAAATATGTGTTCAAAGATAGCACCCGTATTACACTTCCATACTCCGGTAATTACGAAAAGCTTCAAAATGCGGCAGGCAAAAAAAGAGAAAAAATTCCCCTTGGATTCCCAGCTTTGGACAGTGCAATTACCACCTTGTTTCATTACGACTCCACGGCTGCTGCTGCGGCATTTATCGTGGTGATTCAGTGCACGGCTGAGGCTTCAAGATACAAATATATCGAGGCCCAAATGATTAAGAGAATTTCCAAAGACGATGTCCCAAGTCCGGCGATTATCAGTTTAGAAAACAGCTGGTCTGCTCTCTCCAAACAAATTCAATTGGCTCAAACAAATAAAGGAGTTTTTAAAGCTCCGGTTTTGATTACAGACGATCAAGGCCAACGAGTTGAAATAACCAATGTCAGTTCCAACGTTGTAACCAAAAACATCCAGTTGCTACTAAACTCACAAAATATTGCAGCTTTGAGCTACAATTAG

Coding sequence (CDS)

ATGAATGTTGTGTCGGTGTTTTGTTTGGTAATTCTGGCCATCTTCCATGGAAGTTCAAGTTGTGTTGCGGGTGACGTGAGCTTCAACCTGTTGGGTTCGAATTCCAAATCTTATAGCAACTTCATCAAAGCTCTAAGGAGTGCTCTTCCATCCAAGGACAAAGTATACAATATATCACTATTGCTTGGCTCGGCAACAGGCGCAAGTCGCTACACACAAGTGAAACTCTCCAATTATGACGGGAAAGCCATCACGGTGGCAATAGATGTAACAAACGTTTACATCATGGGGTATCTCGTGAACTCAACTTCCTACTTCTTCAACGAATCTGATGCCCAATTGGCTTCTAAATATGTGTTCAAAGATAGCACCCGTATTACACTTCCATACTCCGGTAATTACGAAAAGCTTCAAAATGCGGCAGGCAAAAAAAGAGAAAAAATTCCCCTTGGATTCCCAGCTTTGGACAGTGCAATTACCACCTTGTTTCATTACGACTCCACGGCTGCTGCTGCGGCATTTATCGTGGTGATTCAGTGCACGGCTGAGGCTTCAAGATACAAATATATCGAGGCCCAAATGATTAAGAGAATTTCCAAAGACGATGTCCCAAGTCCGGCGATTATCAGTTTAGAAAACAGCTGGTCTGCTCTCTCCAAACAAATTCAATTGGCTCAAACAAATAAAGGAGTTTTTAAAGCTCCGGTTTTGATTACAGACGATCAAGGCCAACGAGTTGAAATAACCAATGTCAGTTCCAACGTTGTAACCAAAAACATCCAGTTGCTACTAAACTCACAAAATATTGCAGCTTTGAGCTACAATTAG

Protein sequence

MNVVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISLLLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQCTAEASRYKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQGQRVEITNVSSNVVTKNIQLLLNSQNIAALSYN

Homology

[Close]

BLAST of CmaCh17G006110 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 1.2e-104
Identity = 208/266 (78.20%), Postives = 231/266 (86.84%), Query Frame = 0


Query: 7   FCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISLLLGSAT 66
           F ++ILAIF  +S+ V  DV F+L GS+S SYS FI  LR ALPS   VYNI+LLL SA+
Sbjct: 4   FTVLILAIFVAAST-VEADVRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITLLLSSAS 63

Query: 67  GASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRI 126
           GASRYT + LSNYDGKAITVA+DVTNVYIMGYLVNSTSYFFNESDA+LAS+YVFK ST +
Sbjct: 64  GASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIV 123

Query: 127 TLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQCTAEASR 186
           TLPYSGNYEKLQ AAGK REKIPLGFPALDSAITTLFHYDSTAAAAAF+V+IQ TAEASR
Sbjct: 124 TLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASR 183

Query: 187 YKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQGQRV 246
           +KYIE Q+I+RISK+ VPS A ISLEN WSALSKQIQLAQTN G FK PV+ITDD+GQRV
Sbjct: 184 FKYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKGQRV 243

Query: 247 EITNVSSNVVTKNIQLLLN-SQNIAA 272
           EITNV+S VVTKNIQLLLN  QN+AA
Sbjct: 244 EITNVTSKVVTKNIQLLLNYKQNVAA 268

[Close]

BLAST of CmaCh17G006110 vs. ExPASy Swiss-Prot
Match: P84530 (Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=2)

HSP 1 Score: 363.6 bits (932), Expect = 1.9e-99
Identity = 195/241 (80.91%), Postives = 210/241 (87.14%), Query Frame = 0


Query: 25  DVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISLLLGSATGASRYTQVKLSNYDGKAI 84
           DVSF+L GS+S SYS FI ALR ALPS   VYNI+LLL SA+GASRYT +KLSNYDGKAI
Sbjct: 1   DVSFSLSGSSSTSYSKFIGALRKALPSNGTVYNITLLLSSASGASRYTLMKLSNYDGKAI 60

Query: 85  TVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRITLPYSGNYEKLQNAAGKK 144
           TVAIDVTNVYIMGYLVNSTSYFFNESDA+LAS+YVF  ST +TLPYSGNYEKLQ AAGK 
Sbjct: 61  TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFAGSTIVTLPYSGNYEKLQTAAGKI 120

Query: 145 REKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQCTAEASRYKYIEAQMIKRISKDDVP 204
           REKIPLGFPALDSAITTLFHYDSTAAAAAF+V+IQ TAE+SR+KYIE Q+I RISK+ VP
Sbjct: 121 REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAESSRFKYIEGQIIMRISKNGVP 180

Query: 205 SPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQGQRVEITNVSSNVVTKNIQLLL 264
           S A ISLEN WSALSKQIQLAQTN G FK PV+I D  GQRVEI NV S VVTKNIQLLL
Sbjct: 181 SLATISLENEWSALSKQIQLAQTNNGTFKTPVVIMDAGGQRVEIGNVGSKVVTKNIQLLL 240

Query: 265 N 266
           N
Sbjct: 241 N 241

[Close]

BLAST of CmaCh17G006110 vs. ExPASy Swiss-Prot
Match: P22851 (Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 2.1e-93
Identity = 183/249 (73.49%), Postives = 217/249 (87.15%), Query Frame = 0


Query: 25  DVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISLLLGSATGASRYTQVKLSNYDGKAI 84
           +VSF+L G++SKSYS FI ALR ALPSK+KV NI LLL SA+GASRY  ++LSNYD KAI
Sbjct: 2   NVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKAI 61

Query: 85  TVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRITLPYSGNYEKLQNAAGKK 144
           T+AIDVTNVYIMGYLVNSTSYF NESDA+LAS+YVFK ST +T+PYSGNYE+LQNAAGK 
Sbjct: 62  TMAIDVTNVYIMGYLVNSTSYFANESDAKLASQYVFKGSTLVTIPYSGNYERLQNAAGKI 121

Query: 145 REKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQCTAEASRYKYIEAQMIKRISKDDVP 204
           REKIPLGF ALDSA+T++FHYDSTAAAAAF+V++Q TAEASR+KYIE Q+I+RI K++VP
Sbjct: 122 REKIPLGFRALDSALTSIFHYDSTAAAAAFLVILQTTAEASRFKYIEGQIIERIPKNEVP 181

Query: 205 SPAIISLEN-SWSALSKQIQLAQTNKGVFKAPVLITDDQGQRVEITNVSSNVVTK--NIQ 264
           SPA +SLEN +WS LSKQIQLAQTN G F+ PV+I D++GQRVEITN++S V  K  N +
Sbjct: 182 SPAALSLENEAWSLLSKQIQLAQTNNGAFRTPVVIIDNKGQRVEITNLASKVQIKDVNSK 241

Query: 265 LLLNSQNIA 271
           LLLN QNIA
Sbjct: 242 LLLNKQNIA 250

[Close]

BLAST of CmaCh17G006110 vs. ExPASy Swiss-Prot
Match: P16094 (Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=2)

HSP 1 Score: 333.6 bits (854), Expect = 2.2e-90
Identity = 180/271 (66.42%), Postives = 219/271 (80.81%), Query Frame = 0


Query: 1   MNVVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISL 60
           M+  SV   +ILAIF G  S V GDVSF L G++ +SY  FIK LR+ALP ++KVYNI L
Sbjct: 1   MSRFSVLSFLILAIFLG-GSIVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPL 60

Query: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120
           LL S +GA RY  + L NYDGK ITVA+DVTNVYIMGYL ++TSYFFNE  A+LAS+YVF
Sbjct: 61  LLPSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVF 120

Query: 121 KDSTR-ITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQ 180
           +D+ R ITLPYSGNYE+LQ AAGK REKIP+G PALDSAI+TL HYDSTAAA A +V+IQ
Sbjct: 121 RDARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQ 180

Query: 181 CTAEASRYKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLIT 240
            TAEA+R+KYIE Q+ +R  +D+VPS A ISLENSWS LSKQIQLAQ N G+F+ P+++ 
Sbjct: 181 TTAEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLV 240

Query: 241 DDQGQRVEITNVSSNVVTKNIQLLLNSQNIA 271
           D++G RV+ITNV+S VVT NIQLLLN++NIA
Sbjct: 241 DNKGNRVQITNVTSKVVTSNIQLLLNTRNIA 270

[Close]

BLAST of CmaCh17G006110 vs. ExPASy Swiss-Prot
Match: Q9FRX4 (Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 3.6e-85
Identity = 174/274 (63.50%), Postives = 215/274 (78.47%), Query Frame = 0


Query: 1   MNVVSV-FCLVILAIFHGSSSCVAGDVS--FNLLGSNSKSYSNFIKALRSALPSKDKVYN 60
           MN  SV  CLVIL+IFHG  +   GDV+  F+LLGSN KSYS FI ++R+ALP+   +YN
Sbjct: 1   MNRFSVLMCLVILSIFHGVPT-AEGDVTVKFSLLGSNHKSYSKFITSMRNALPNAGDIYN 60

Query: 61  ISLLLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASK 120
           I LL+ S +G+ RY  ++LSNY+G  IT+A+DVTNVYIMGYLVN TSYFFNE+DAQLASK
Sbjct: 61  IPLLVPSISGSRRYILMQLSNYEGNTITMAVDVTNVYIMGYLVNGTSYFFNETDAQLASK 120

Query: 121 YVFKDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVV 180
           +VF+ +  ITLPYSGNY+KLQ+ A K+R+ IPLGF ALDSAI+TL++YDS +A  AF+V+
Sbjct: 121 FVFQGTKSITLPYSGNYQKLQSVARKERDSIPLGFMALDSAISTLYYYDSRSAPIAFLVL 180

Query: 181 IQCTAEASRYKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVL 240
           IQ TAEA+RYKYIE Q+I RIS   VP  A ISLEN WS LSKQIQ+A++N G F+ PV 
Sbjct: 181 IQTTAEAARYKYIEKQIIDRISVSKVPDLAAISLENEWSLLSKQIQIAKSNNGQFQTPVK 240

Query: 241 ITDDQGQRVEITNVSSNVVTKNIQLLLNSQNIAA 272
           I +D+G   E+TNVSS VVTKNI LLLN  NIA+
Sbjct: 241 IINDKGILTEVTNVSSLVVTKNIMLLLNKLNIAS 273

[Close]

BLAST of CmaCh17G006110 vs. ExPASy TrEMBL
Match: A0A6J1JVG7 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488687 PE=3 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 1.7e-143
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0


Query: 1   MNVVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISL 60
           MNVVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISL
Sbjct: 11  MNVVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISL 70

Query: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120
           LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF
Sbjct: 71  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 130

Query: 121 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQC 180
           KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQC
Sbjct: 131 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQC 190

Query: 181 TAEASRYKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 240
           TAEASRYKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD
Sbjct: 191 TAEASRYKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 250

Query: 241 DQGQRVEITNVSSNVVTKNIQLLLNSQNIAALSYN 276
           DQGQRVEITNVSSNVVTKNIQLLLNSQNIAALSYN
Sbjct: 251 DQGQRVEITNVSSNVVTKNIQLLLNSQNIAALSYN 285

[Close]

BLAST of CmaCh17G006110 vs. ExPASy TrEMBL
Match: A0A6J1GMM2 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455894 PE=3 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 4.9e-138
Identity = 266/275 (96.73%), Postives = 270/275 (98.18%), Query Frame = 0


Query: 1   MNVVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISL 60
           MN VSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFI+ALRSALPSKDKVYNISL
Sbjct: 11  MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 70

Query: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120
           LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF
Sbjct: 71  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 130

Query: 121 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQC 180
           KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALD+AITTLFHYDSTAAAAAFIVVIQC
Sbjct: 131 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 190

Query: 181 TAEASRYKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 240
           TAEASRY+YIEAQMIKRISKDDVPS AIISLENSWSALSKQIQLAQTNKGVFKAPVLITD
Sbjct: 191 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 250

Query: 241 DQGQRVEITNVSSNVVTKNIQLLLNSQNIAALSYN 276
           DQGQRVEITNVSS VV KNIQLLLNS+NIA LSYN
Sbjct: 251 DQGQRVEITNVSSKVVAKNIQLLLNSKNIADLSYN 285

[Close]

BLAST of CmaCh17G006110 vs. ExPASy TrEMBL
Match: B0EVM6 (rRNA N-glycosidase OS=Luffa acutangula OX=56866 GN=RIPI PE=2 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 8.0e-96
Identity = 192/263 (73.00%), Postives = 223/263 (84.79%), Query Frame = 0


Query: 9   LVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISLLLGSATGA 68
           L +L I     +    +VSF+L G++SKSYS FI ALR ALPSK+KV NI LLL SA+GA
Sbjct: 7   LSLLIILIAFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGA 66

Query: 69  SRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRITL 128
           SRY  ++LSNYD KAIT+AIDVTNVYIMGYLVNSTSYFFNESDA+LAS+YVFK ST +TL
Sbjct: 67  SRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTL 126

Query: 129 PYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQCTAEASRYK 188
           PYSGNYE+LQNAAGK REKIPLGF A DSAIT+LFHYDSTAAA AF+V+IQ TAEASR+K
Sbjct: 127 PYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTAEASRFK 186

Query: 189 YIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQGQRVEI 248
           YIE Q+I+RI K++VPSPA +SLEN WSALSKQIQLAQTN G F+ PV+I D++GQRVEI
Sbjct: 187 YIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNKGQRVEI 246

Query: 249 TNVSSNVVTKNIQLLLNSQNIAA 272
            +V+S VVT NI+LLLN QNIAA
Sbjct: 247 KDVNSKVVTNNIKLLLNKQNIAA 269

[Close]

BLAST of CmaCh17G006110 vs. ExPASy TrEMBL
Match: Q00980 (rRNA N-glycosidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 8.0e-96
Identity = 192/263 (73.00%), Postives = 223/263 (84.79%), Query Frame = 0


Query: 9   LVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISLLLGSATGA 68
           L +L I     +    +VSF+L G++SKSYS FI ALR ALPSK+KV NI LLL SA+GA
Sbjct: 7   LSLLIILIAFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGA 66

Query: 69  SRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRITL 128
           SRY  ++LSNYD KAIT+AIDVTNVYIMGYLVNSTSYFFNESDA+LAS+YVFK ST +TL
Sbjct: 67  SRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTL 126

Query: 129 PYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQCTAEASRYK 188
           PYSGNYE+LQNAAGK REKIPLGF A DSAIT+LFHYDSTAAA AF+V+IQ TAEASR+K
Sbjct: 127 PYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQTTAEASRFK 186

Query: 189 YIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQGQRVEI 248
           YIE Q+I+RI K++VPSPA +SLEN WSALSKQIQLAQTN G F+ PV+I D++GQRVEI
Sbjct: 187 YIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIIDNKGQRVEI 246

Query: 249 TNVSSNVVTKNIQLLLNSQNIAA 272
            +V+S VVT NI+LLLN QNIAA
Sbjct: 247 KDVNSKVVTNNIKLLLNKQNIAA 269

[Close]

BLAST of CmaCh17G006110 vs. ExPASy TrEMBL
Match: A0A6J1DSN8 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023551 PE=3 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 8.0e-88
Identity = 180/271 (66.42%), Postives = 219/271 (80.81%), Query Frame = 0


Query: 1   MNVVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISL 60
           M+  SV   +ILAIF G  S V GDVSF L G++ +SY  FIK LR+ALP ++KVYNI L
Sbjct: 1   MSRFSVLSFLILAIFLG-GSIVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPL 60

Query: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120
           LL S +GA RY  + L NYDGK ITVA+DVTNVYIMGYL ++TSYFFNE  A+LAS+YVF
Sbjct: 61  LLPSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVF 120

Query: 121 KDSTR-ITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQ 180
           +D+ R ITLPYSGNYE+LQ AAGK REKIP+G PALDSAI+TL HYDSTAAA A +V+IQ
Sbjct: 121 RDARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQ 180

Query: 181 CTAEASRYKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLIT 240
            TAEA+R+KYIE Q+ +R  +D+VPS A ISLENSWS LSKQIQLAQ N G+F+ P+++ 
Sbjct: 181 TTAEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLV 240

Query: 241 DDQGQRVEITNVSSNVVTKNIQLLLNSQNIA 271
           D++G RV+ITNV+S VVT NIQLLLN++NIA
Sbjct: 241 DNKGNRVQITNVTSKVVTSNIQLLLNTRNIA 270

[Close]

BLAST of CmaCh17G006110 vs. NCBI nr
Match: XP_022992350.1 (ribosome-inactivating protein luffaculin 1-like [Cucurbita maxima])

HSP 1 Score: 518.5 bits (1334), Expect = 3.6e-143
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0


Query: 1   MNVVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISL 60
           MNVVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISL
Sbjct: 11  MNVVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISL 70

Query: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120
           LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF
Sbjct: 71  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 130

Query: 121 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQC 180
           KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQC
Sbjct: 131 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQC 190

Query: 181 TAEASRYKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 240
           TAEASRYKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD
Sbjct: 191 TAEASRYKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 250

Query: 241 DQGQRVEITNVSSNVVTKNIQLLLNSQNIAALSYN 276
           DQGQRVEITNVSSNVVTKNIQLLLNSQNIAALSYN
Sbjct: 251 DQGQRVEITNVSSNVVTKNIQLLLNSQNIAALSYN 285

[Close]

BLAST of CmaCh17G006110 vs. NCBI nr
Match: XP_022953306.1 (ribosome-inactivating protein luffaculin 1-like [Cucurbita moschata])

HSP 1 Score: 500.4 bits (1287), Expect = 1.0e-137
Identity = 266/275 (96.73%), Postives = 270/275 (98.18%), Query Frame = 0


Query: 1   MNVVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISL 60
           MN VSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFI+ALRSALPSKDKVYNISL
Sbjct: 11  MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 70

Query: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120
           LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF
Sbjct: 71  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 130

Query: 121 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQC 180
           KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALD+AITTLFHYDSTAAAAAFIVVIQC
Sbjct: 131 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 190

Query: 181 TAEASRYKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 240
           TAEASRY+YIEAQMIKRISKDDVPS AIISLENSWSALSKQIQLAQTNKGVFKAPVLITD
Sbjct: 191 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 250

Query: 241 DQGQRVEITNVSSNVVTKNIQLLLNSQNIAALSYN 276
           DQGQRVEITNVSS VV KNIQLLLNS+NIA LSYN
Sbjct: 251 DQGQRVEITNVSSKVVAKNIQLLLNSKNIADLSYN 285

[Close]

BLAST of CmaCh17G006110 vs. NCBI nr
Match: KAG6575376.1 (hypothetical protein SDJN03_26015, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013917.1 hypothetical protein SDJN02_24086, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 500.4 bits (1287), Expect = 1.0e-137
Identity = 266/275 (96.73%), Postives = 270/275 (98.18%), Query Frame = 0


Query: 1   MNVVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISL 60
           MN VSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFI+ALRSALPSKDKVYNISL
Sbjct: 1   MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 60

Query: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120
           LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF
Sbjct: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120

Query: 121 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQC 180
           KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALD+AITTLFHYDSTAAAAAFIVVIQC
Sbjct: 121 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 180

Query: 181 TAEASRYKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 240
           TAEASRY+YIEAQMIKRISKDDVPS AIISLENSWSALSKQIQLAQTNKGVFKAPVLITD
Sbjct: 181 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 240

Query: 241 DQGQRVEITNVSSNVVTKNIQLLLNSQNIAALSYN 276
           DQGQRVEITNVSS VV KNIQLLLNS+NIA LSYN
Sbjct: 241 DQGQRVEITNVSSKVVAKNIQLLLNSKNIADLSYN 275

[Close]

BLAST of CmaCh17G006110 vs. NCBI nr
Match: XP_023520860.1 (ribosome-inactivating protein luffaculin 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 498.0 bits (1281), Expect = 5.1e-137
Identity = 264/275 (96.00%), Postives = 270/275 (98.18%), Query Frame = 0


Query: 1   MNVVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISL 60
           MN VSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFI+ALRSALPSKDKVYNISL
Sbjct: 11  MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 70

Query: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120
           L+GSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF
Sbjct: 71  LVGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 130

Query: 121 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQC 180
           KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALD+AITTLFHYDSTAAAAAF+VVIQC
Sbjct: 131 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFMVVIQC 190

Query: 181 TAEASRYKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 240
           TAEASRY+YIEAQMIKRISKDDVPS AIISLENSWSALSKQIQLAQTNKGVFKAPVLITD
Sbjct: 191 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 250

Query: 241 DQGQRVEITNVSSNVVTKNIQLLLNSQNIAALSYN 276
           DQGQRVEITNVSS VV KNIQLLLNS+NIA LSYN
Sbjct: 251 DQGQRVEITNVSSKVVAKNIQLLLNSKNIADLSYN 285

[Close]

BLAST of CmaCh17G006110 vs. NCBI nr
Match: Q00465.1 (RecName: Full=Ribosome-inactivating protein luffin-alpha; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Luffa aegyptiaca] >CAA44229.1 a-luffin [Luffa aegyptiaca])

HSP 1 Score: 380.9 bits (977), Expect = 9.0e-102
Identity = 208/266 (78.20%), Postives = 231/266 (86.84%), Query Frame = 0


Query: 7   FCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISLLLGSAT 66
           F ++ILAIF  +S+ V  DV F+L GS+S SYS FI  LR ALPS   VYNI+LLL SA+
Sbjct: 4   FTVLILAIFVAAST-VEADVRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITLLLSSAS 63

Query: 67  GASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRI 126
           GASRYT + LSNYDGKAITVA+DVTNVYIMGYLVNSTSYFFNESDA+LAS+YVFK ST +
Sbjct: 64  GASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIV 123

Query: 127 TLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQCTAEASR 186
           TLPYSGNYEKLQ AAGK REKIPLGFPALDSAITTLFHYDSTAAAAAF+V+IQ TAEASR
Sbjct: 124 TLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASR 183

Query: 187 YKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQGQRV 246
           +KYIE Q+I+RISK+ VPS A ISLEN WSALSKQIQLAQTN G FK PV+ITDD+GQRV
Sbjct: 184 FKYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKGQRV 243

Query: 247 EITNVSSNVVTKNIQLLLN-SQNIAA 272
           EITNV+S VVTKNIQLLLN  QN+AA
Sbjct: 244 EITNVTSKVVTKNIQLLLNYKQNVAA 268

The following BLAST results are available for this feature:

BLAST of CmaCh17G006110 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q00465	1.2e-104	78.20	Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P84530	1.9e-99	80.91	Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=...	[more]
P22851	2.1e-93	73.49	Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P16094	2.2e-90	66.42	Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=...	[more]
Q9FRX4	3.6e-85	63.50	Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1	[more]

BLAST of CmaCh17G006110 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JVG7	1.7e-143	100.00	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488687 PE=3 SV=1	[more]
A0A6J1GMM2	4.9e-138	96.73	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455894 PE=3 SV=1	[more]
B0EVM6	8.0e-96	73.00	rRNA N-glycosidase OS=Luffa acutangula OX=56866 GN=RIPI PE=2 SV=1	[more]
Q00980	8.0e-96	73.00	rRNA N-glycosidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1	[more]
A0A6J1DSN8	8.0e-88	66.42	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023551 PE=3 SV=1	[more]

BLAST of CmaCh17G006110 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022992350.1	3.6e-143	100.00	ribosome-inactivating protein luffaculin 1-like [Cucurbita maxima]	[more]
XP_022953306.1	1.0e-137	96.73	ribosome-inactivating protein luffaculin 1-like [Cucurbita moschata]	[more]
KAG6575376.1	1.0e-137	96.73	hypothetical protein SDJN03_26015, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023520860.1	5.1e-137	96.00	ribosome-inactivating protein luffaculin 1-like [Cucurbita pepo subsp. pepo]	[more]
Q00465.1	9.0e-102	78.20	RecName: Full=Ribosome-inactivating protein luffin-alpha; AltName: Full=rRNA N-g...	[more]

BLAST of CmaCh17G006110 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 145..159 score: 56.84 coord: 174..194 score: 50.13 coord: 206..223 score: 53.65 coord: 83..98 score: 52.3 coord: 36..49 score: 45.68
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 24..185 e-value: 6.1E-47 score: 161.3
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 35..223 e-value: 2.9E-32 score: 111.9
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 27..268
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 186..270 e-value: 1.4E-27 score: 97.4
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 27..268
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 25..268

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh17G006110.1	CmaCh17G006110.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051607	defense response to virus
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity