CmaCh06G000210 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh06G000210 vs. ExPASy Swiss-Prot
Match: F4I171 (Mediator of RNA polymerase II transcription subunit 15a OS=Arabidopsis thaliana OX=3702 GN=MED15A PE=1 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 7.5e-31
Identity = 102/301 (33.89%), Postives = 150/301 (49.83%), Query Frame = 0

Query: 434  NGQLSTTTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRIT-------------EPW 493
            +G+ S T  P  RL++AV+SIS +AL +A+ +I SV +M DRI              E  
Sbjct: 1037 SGKPSATELPIERLIRAVKSISPQALSSAVSDIGSVVSMVDRIAGSAPGNGSRASVGEDL 1096

Query: 494  FCEKETHLS-----LQDGGGFSKNMKRKMNATTL-----------NDMPSPCSDIVGSEP 553
                +  L       Q+G   +K MKR   A  L           N      S+    E 
Sbjct: 1097 VAMTKCRLQARNFMTQEGMMATKKMKRHTTAMPLSVASLGGSVGDNYKQFAGSETSDLES 1156

Query: 554  TVTSISKKLKKLSDNALLEEMRNINRHFIETVLELDTDENVN-------RRLANAGTVLR 613
            T TS  KK +  +++ALLEE++ IN+  I+TV+E+  DE+           +   GT +R
Sbjct: 1157 TATSDGKKARTETEHALLEEIKEINQRLIDTVVEISDDEDAADPSEVAISSIGCEGTTVR 1216

Query: 614  CSYRGATEGKNSVFHSENNTLKLPVLSVKLLVPLDYPEDYPVFLSKLNTDCGTEDGEFRD 673
             S+   +       H  ++T   P+  ++LLVP  YP   P  L KL  +   E+    D
Sbjct: 1217 FSFIAVSLSPALKAHL-SSTQMSPIQPLRLLVPCSYPNGSPSLLDKLPVETSKEN---ED 1276

Query: 674  MWSKATSLLSAFLRTSPVYLSLEECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWEDCA 699
            + SKA +  +  LR+    +SL++ AK WD CARAV+C+YAQ+ GGG FSS+YG+WE   
Sbjct: 1277 LSSKAMARFNILLRSLSQPMSLKDIAKTWDACARAVICEYAQQFGGGTFSSKYGTWEKYV 1333

BLAST of CmaCh06G000210 vs. ExPASy Swiss-Prot
Match: Q9SHV7 (Probable mediator of RNA polymerase II transcription subunit 15c OS=Arabidopsis thaliana OX=3702 GN=MED15C PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 6.6e-19
Identity = 109/465 (23.44%), Postives = 205/465 (44.09%), Query Frame = 0

Query: 271 PREIGSLRSEADWIQKYTPRNRQHSEKIEQEFKSSWIKQNQKSTENIQAIRR------SG 330
           PR + +  S    +QK   + R H  +++Q+       Q+Q  T  +  +R         
Sbjct: 487 PRAVNTNISSVQSLQK---QKRFHHRQMQQQQPQQGNHQHQMQTNEMNDVRMRERVNIKA 546

Query: 331 MYLENHFNS--KFFPQTHEASRLSQIAERALPKIACNSLYGRASPTSPSTDGLGKISSNV 390
             LE   +S  +  P+       SQI   + P++    +     P + +  G   ++S+ 
Sbjct: 547 RLLEQQVSSSQRQVPKQESNVSSSQIQNHSSPQLVDQHIL----PATINKTGT-PLNSSG 606

Query: 391 SSLWSPISQFSRGYSSLKLLNSKSEIEVP---SHEIRSYNTMTCRLA--ETTRFGNNGQL 450
           S+  +P      G S + +      +E P     EI S    + +L   ET         
Sbjct: 607 SAFVAPAPSPVPGDSEMPI-----SVESPVSGVDEINSTLDSSSKLGTQETPLLFVPPPE 666

Query: 451 STTTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW-------------FCEK 510
             T +P +RL+KA ++ S ++L  ++ EISSV +M D I   +               E+
Sbjct: 667 PITERPIDRLIKAFQAASPKSLAESVSEISSVISMVDMIGGSFPSSGGSRAGLGEDLSER 726

Query: 511 ETHLSLQDGGGFSKNMKRKMN-----ATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDN 570
             + +  +    SK MKR +N      ++  D     S +     + TS   K+  ++  
Sbjct: 727 TRNFTTHEETNLSKRMKRSINIVPPDMSSQIDSYEQLSSLESEVVSTTSSGLKVNNIAPG 786

Query: 571 -ALLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSY---------RGATEGKNS 630
            ALL+E++  N   +ETV+E+  ++++       GT++ C+Y         +   +    
Sbjct: 787 YALLQEIKETNGRLVETVVEICDEDSL-------GTIVTCTYAPVALSATFKDHYKSGKI 846

Query: 631 VFHSENNTLKLPVLSVKLLVPLDYPEDYPVFLSKLNTDCGTEDGEFRDMWSKATSLLSAF 690
           +F+     ++  +  ++LL P+DYP   P+ L +++ D  T   ++ D+ ++  S  S  
Sbjct: 847 IFYVSKCLMQAQIQPLRLLFPMDYPYSSPIVLEEISFD--TSVHKYEDLSARTRSRFSLS 906

Query: 691 LRTSPVYLSLEECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWE 695
           ++        +  A+ W++CARA + +YA+R GGG FSS+YG+WE
Sbjct: 907 MKEFSEPGFSKGIAQTWNDCARATMVEYAERHGGGTFSSKYGAWE 929

BLAST of CmaCh06G000210 vs. ExPASy TrEMBL
Match: A0A6J1I7U8 (mediator of RNA polymerase II transcription subunit 15a isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111469984 PE=4 SV=1)

HSP 1 Score: 1391.3 bits (3600), Expect = 0.0e+00
Identity = 699/699 (100.00%), Postives = 699/699 (100.00%), Query Frame = 0

Query: 1   MEHEKKLFKNANSEEQHIPQSLSQPHERLHGQKLKVRQTHQQYVMHNQSCVKPQNILTAP 60
           MEHEKKLFKNANSEEQHIPQSLSQPHERLHGQKLKVRQTHQQYVMHNQSCVKPQNILTAP
Sbjct: 1   MEHEKKLFKNANSEEQHIPQSLSQPHERLHGQKLKVRQTHQQYVMHNQSCVKPQNILTAP 60

Query: 61  CRPQGFQSRAFGNHMPPEMFTQHPKVVNLQPNENLSAQVKEEVIDEDFIGSTFLHQHYSA 120
           CRPQGFQSRAFGNHMPPEMFTQHPKVVNLQPNENLSAQVKEEVIDEDFIGSTFLHQHYSA
Sbjct: 61  CRPQGFQSRAFGNHMPPEMFTQHPKVVNLQPNENLSAQVKEEVIDEDFIGSTFLHQHYSA 120

Query: 121 IEQHKQHQSVGASAVPNGNPNAEDWHDLALSEVQRLRTHLPLLKKAYVRASEQCLQVGQT 180
           IEQHKQHQSVGASAVPNGNPNAEDWHDLALSEVQRLRTHLPLLKKAYVRASEQCLQVGQT
Sbjct: 121 IEQHKQHQSVGASAVPNGNPNAEDWHDLALSEVQRLRTHLPLLKKAYVRASEQCLQVGQT 180

Query: 181 EKMEKYEHDKNILQKIINFLKLPEDRIISYTKESFYRCVQTIEETLKVHEKGSINIASKK 240
           EKMEKYEHDKNILQKIINFLKLPEDRIISYTKESFYRCVQTIEETLKVHEKGSINIASKK
Sbjct: 181 EKMEKYEHDKNILQKIINFLKLPEDRIISYTKESFYRCVQTIEETLKVHEKGSINIASKK 240

Query: 241 QPLHGQPSLCQSHTNPTQQTDSSDSSSPRAPREIGSLRSEADWIQKYTPRNRQHSEKIEQ 300
           QPLHGQPSLCQSHTNPTQQTDSSDSSSPRAPREIGSLRSEADWIQKYTPRNRQHSEKIEQ
Sbjct: 241 QPLHGQPSLCQSHTNPTQQTDSSDSSSPRAPREIGSLRSEADWIQKYTPRNRQHSEKIEQ 300

Query: 301 EFKSSWIKQNQKSTENIQAIRRSGMYLENHFNSKFFPQTHEASRLSQIAERALPKIACNS 360
           EFKSSWIKQNQKSTENIQAIRRSGMYLENHFNSKFFPQTHEASRLSQIAERALPKIACNS
Sbjct: 301 EFKSSWIKQNQKSTENIQAIRRSGMYLENHFNSKFFPQTHEASRLSQIAERALPKIACNS 360

Query: 361 LYGRASPTSPSTDGLGKISSNVSSLWSPISQFSRGYSSLKLLNSKSEIEVPSHEIRSYNT 420
           LYGRASPTSPSTDGLGKISSNVSSLWSPISQFSRGYSSLKLLNSKSEIEVPSHEIRSYNT
Sbjct: 361 LYGRASPTSPSTDGLGKISSNVSSLWSPISQFSRGYSSLKLLNSKSEIEVPSHEIRSYNT 420

Query: 421 MTCRLAETTRFGNNGQLSTTTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW 480
           MTCRLAETTRFGNNGQLSTTTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW
Sbjct: 421 MTCRLAETTRFGNNGQLSTTTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW 480

Query: 481 FCEKETHLSLQDGGGFSKNMKRKMNATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDNA 540
           FCEKETHLSLQDGGGFSKNMKRKMNATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDNA
Sbjct: 481 FCEKETHLSLQDGGGFSKNMKRKMNATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDNA 540

Query: 541 LLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSYRGATEGKNSVFHSENNTLKL 600
           LLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSYRGATEGKNSVFHSENNTLKL
Sbjct: 541 LLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSYRGATEGKNSVFHSENNTLKL 600

Query: 601 PVLSVKLLVPLDYPEDYPVFLSKLNTDCGTEDGEFRDMWSKATSLLSAFLRTSPVYLSLE 660
           PVLSVKLLVPLDYPEDYPVFLSKLNTDCGTEDGEFRDMWSKATSLLSAFLRTSPVYLSLE
Sbjct: 601 PVLSVKLLVPLDYPEDYPVFLSKLNTDCGTEDGEFRDMWSKATSLLSAFLRTSPVYLSLE 660

Query: 661 ECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWEDCAAT 700
           ECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWEDCAAT
Sbjct: 661 ECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWEDCAAT 699

BLAST of CmaCh06G000210 vs. ExPASy TrEMBL
Match: A0A6J1EZU5 (mediator of RNA polymerase II transcription subunit 15a isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111440856 PE=4 SV=1)

HSP 1 Score: 1263.4 bits (3268), Expect = 0.0e+00
Identity = 643/699 (91.99%), Postives = 655/699 (93.71%), Query Frame = 0

Query: 1   MEHEKKLFKNANSEEQHIPQSLSQPHERLHGQKLKVRQTHQQYVMHNQSCVKPQNILTAP 60
           MEHEKKLFKNANS+EQHIPQSLSQPHERLHGQKLKVRQTHQQY+MHNQSCV+PQN LTAP
Sbjct: 1   MEHEKKLFKNANSKEQHIPQSLSQPHERLHGQKLKVRQTHQQYLMHNQSCVRPQNTLTAP 60

Query: 61  CRPQGFQSRAFGNHMPPEMFTQHPKVVNLQPNENLSAQVKEEVIDEDFIGSTFLHQHYSA 120
           CRPQGFQSRAFGNHMPPEMFTQHPKVVNLQPNENLSAQVKEEVIDEDFIGSTFLHQ+YSA
Sbjct: 61  CRPQGFQSRAFGNHMPPEMFTQHPKVVNLQPNENLSAQVKEEVIDEDFIGSTFLHQYYSA 120

Query: 121 IEQHKQHQSVGASAVPNGNPNAEDWHDLALSEVQRLRTHLPLLKKAYVRASEQCLQVGQT 180
           IEQHKQHQ+VGASAVPNGNPNAEDWHDLALSEVQRLRTHLPLL KAYVRASEQCLQVGQT
Sbjct: 121 IEQHKQHQTVGASAVPNGNPNAEDWHDLALSEVQRLRTHLPLLTKAYVRASEQCLQVGQT 180

Query: 181 EKMEKYEHDKNILQKIINFLKLPEDRIISYTKESFYRCVQTIEETLKVHEKGSINIASKK 240
           EKM+KYEHDKNILQKIINFLKLPEDRIISYTKESFYRCVQTIEETLKVHE GSINIASK+
Sbjct: 181 EKMQKYEHDKNILQKIINFLKLPEDRIISYTKESFYRCVQTIEETLKVHENGSINIASKR 240

Query: 241 QPLHGQPSLCQSHTNPTQQTDSSDSSSPRAPREIGSLRSEADWIQKYTPRNRQHSEKIEQ 300
           QPLHGQPSLCQSH NP QQT+S DSSSPRAPREIGSLRSEADWIQKYTPRNRQHSEKIEQ
Sbjct: 241 QPLHGQPSLCQSHINPAQQTNSFDSSSPRAPREIGSLRSEADWIQKYTPRNRQHSEKIEQ 300

Query: 301 EFKSSWIKQNQKSTENIQAIRRSGMYLENHFNSKFFPQTHEASRLSQIAERALPKIACNS 360
           EFKSSWIKQNQKSTENIQAIRRSGMYLENHFNSKF PQTHEASRLSQIAERALPKIACNS
Sbjct: 301 EFKSSWIKQNQKSTENIQAIRRSGMYLENHFNSKFSPQTHEASRLSQIAERALPKIACNS 360

Query: 361 LYGRASPTSPSTDGLGKISSNVSSLWSPISQFSRGYSSLKLLNSKSEIEVPSHEIRSYNT 420
           LYGRASPT PSTDGLGKISSNVSSLWSPISQFS+                          
Sbjct: 361 LYGRASPTFPSTDGLGKISSNVSSLWSPISQFSQ-------------------------- 420

Query: 421 MTCRLAETTRFGNNGQLSTTTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW 480
                 ETTRFGNNGQLS TTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW
Sbjct: 421 ------ETTRFGNNGQLS-TTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW 480

Query: 481 FCEKETHLSLQDGGGFSKNMKRKMNATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDNA 540
           FCEKETHLSLQDGGG SKNMKRKMNATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDNA
Sbjct: 481 FCEKETHLSLQDGGGSSKNMKRKMNATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDNA 540

Query: 541 LLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSYRGATEGKNSVFHSENNTLKL 600
           LLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSYR ATEGKNSVFHSENNTLKL
Sbjct: 541 LLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSYRAATEGKNSVFHSENNTLKL 600

Query: 601 PVLSVKLLVPLDYPEDYPVFLSKLNTDCGTEDGEFRDMWSKATSLLSAFLRTSPVYLSLE 660
           PVLSVKLLVPLDYPEDYPVFLSKLNT+CGTED EFRDMWSKATSLL+AFLRT+PVYLSLE
Sbjct: 601 PVLSVKLLVPLDYPEDYPVFLSKLNTECGTEDEEFRDMWSKATSLLNAFLRTAPVYLSLE 660

Query: 661 ECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWEDCAAT 700
           ECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWEDCAAT
Sbjct: 661 ECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWEDCAAT 666

BLAST of CmaCh06G000210 vs. ExPASy TrEMBL
Match: A0A6J1D158 (probable mediator of RNA polymerase II transcription subunit 15c isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016654 PE=4 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 9.4e-218
Identity = 430/715 (60.14%), Postives = 524/715 (73.29%), Query Frame = 0

Query: 16  QHIPQSLSQPHERLHGQKLKVRQTHQQYVMHNQSCVKPQNILTAPCRPQGFQSRAFGNHM 75
           Q+I Q   Q HE+L  Q L  RQ HQQ+ MH+QSCV+PQN L +PCRP G QSR  G ++
Sbjct: 148 QNIMQLTPQSHEQLQRQNLNARQLHQQFGMHSQSCVRPQNTLISPCRPLGLQSRDSGVYI 207

Query: 76  PPEMFTQHPKVVNLQPNENLSAQVKEEVIDEDFIGSTFLHQHY------SAIEQHKQHQS 135
           PP+MF  H +V+NLQPNENL AQ+KEEVI ED   S F+  H+      + IEQHKQHQS
Sbjct: 208 PPQMFPPHSEVMNLQPNENLRAQIKEEVIGEDVQASKFVQPHHTPRGPNTMIEQHKQHQS 267

Query: 136 VGASAVPNGNPNAEDWHDLALSEVQRLR-THLPLLKKAYVRASEQCLQVGQTEKMEKYEH 195
           +G SAV   NP  EDWHD A +E+Q L+ T LPLLK++Y RA++ CL+VGQTE+++K ++
Sbjct: 268 MGVSAVLIENPKGEDWHDQAYNEMQGLKMTCLPLLKESYERAAKLCLEVGQTEQIQKCKN 327

Query: 196 DKNILQKIINFLKLPEDRIISYTKESFYRCVQTIEETLKVHEKGSINIASKKQPLHGQPS 255
             +++QK+ NFL+LP D+I  +TKE FY+ +++IE+  K HE  +  + +K+QPLHGQP 
Sbjct: 328 HMSLMQKMTNFLELPRDKITYFTKEKFYQSMKSIEKFAKAHENRNTFLVNKQQPLHGQPG 387

Query: 256 LCQSHTNPTQQTD---------------SSDSSSPRAPREIGSLRSEADWIQKYTPRNRQ 315
           + QSH NP Q++D               SSDSSSPR P EIGS R EA+ IQK   + RQ
Sbjct: 388 ISQSHINPVQRSDNANPHFQPLNLAATGSSDSSSPRTPSEIGSSRPEANRIQKNLLQKRQ 447

Query: 316 HSEKIEQEFKSSWIKQNQKSTENIQAIRRSGMYLENHFNSKFFPQTH-EASRLSQIAERA 375
             E I+QEF+SSWI+Q QKSTE+IQAI RSG+ L+NH NSK  PQ+H EAS+LS+IAERA
Sbjct: 448 QPEIIKQEFQSSWIQQMQKSTESIQAINRSGVSLQNHLNSKHSPQSHEEASQLSKIAERA 507

Query: 376 LPKIACNSLY---GRASPT-SPSTDGLGKISSNVSSLWSPISQFSRGYSSLKLLNSKSEI 435
           L K  C+S+Y   GRASPT S ST GLGK SSNVS L S   Q+    +S+ LLNSK++I
Sbjct: 508 LSKDPCSSVYGRDGRASPTPSSSTVGLGKSSSNVSCLSSLNFQYPETRNSVNLLNSKTKI 567

Query: 436 EVPSHEIRSYNTMTCRLAETTRFGNNGQLSTTTQPHNRLLKAVESISNEALRAAILEISS 495
           +V SHEIRS    T + AE T  G++  LST TQP NRLLKAV+S+S++ALR AI  ISS
Sbjct: 568 QVKSHEIRSSGISTSQFAEPTSLGSSQHLSTATQPLNRLLKAVDSMSDQALRTAISGISS 627

Query: 496 VTNMEDRITEPWF----C-EKETHLSLQDGGGFSKNMKRKMNATTLNDMPSPCSDIVGSE 555
           V NM D +TEP      C +K  HLSLQDG G S NMKRK+ A TLNDMPSPCSD  GSE
Sbjct: 628 VGNMCDTVTEPSVFGTRCHQKAAHLSLQDGFGSSNNMKRKICAITLNDMPSPCSDNDGSE 687

Query: 556 PTVTSISKKLKKLSDNALLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSYRGA 615
            TVTS SKKLKKL+DNALLEEMRNIN+  +ETVLELD+ +NVNRR ANAGTV+RC+Y   
Sbjct: 688 LTVTSRSKKLKKLTDNALLEEMRNINQRLVETVLELDSAQNVNRRFANAGTVVRCTYSAV 747

Query: 616 TEGKNSVFHSENNTLKLPVLSVKLLVPLDYPEDYPVFLSKLNTDCGTEDGEFRDMWSKAT 675
           ++  N +FH   NTLKLPVLSVKLLVPLDYPEDYPVFLSK NTDCG ED E RD+  KAT
Sbjct: 748 SDRNNLMFHFA-NTLKLPVLSVKLLVPLDYPEDYPVFLSKFNTDCGNEDEECRDLSRKAT 807

Query: 676 SLLSAFLRTSPVYLSLEECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWEDCAA 699
           S+L AFLRT+P  LSL E A+AWD+CAR VV +YAQR GGGCFSSRYG+WEDC A
Sbjct: 808 SMLRAFLRTAPERLSLGEYARAWDQCARYVVSEYAQRTGGGCFSSRYGTWEDCVA 861

BLAST of CmaCh06G000210 vs. ExPASy TrEMBL
Match: A0A6J1D3G7 (mediator of RNA polymerase II transcription subunit 15a-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016654 PE=4 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 9.4e-218
Identity = 430/715 (60.14%), Postives = 524/715 (73.29%), Query Frame = 0

Query: 16  QHIPQSLSQPHERLHGQKLKVRQTHQQYVMHNQSCVKPQNILTAPCRPQGFQSRAFGNHM 75
           Q+I Q   Q HE+L  Q L  RQ HQQ+ MH+QSCV+PQN L +PCRP G QSR  G ++
Sbjct: 93  QNIMQLTPQSHEQLQRQNLNARQLHQQFGMHSQSCVRPQNTLISPCRPLGLQSRDSGVYI 152

Query: 76  PPEMFTQHPKVVNLQPNENLSAQVKEEVIDEDFIGSTFLHQHY------SAIEQHKQHQS 135
           PP+MF  H +V+NLQPNENL AQ+KEEVI ED   S F+  H+      + IEQHKQHQS
Sbjct: 153 PPQMFPPHSEVMNLQPNENLRAQIKEEVIGEDVQASKFVQPHHTPRGPNTMIEQHKQHQS 212

Query: 136 VGASAVPNGNPNAEDWHDLALSEVQRLR-THLPLLKKAYVRASEQCLQVGQTEKMEKYEH 195
           +G SAV   NP  EDWHD A +E+Q L+ T LPLLK++Y RA++ CL+VGQTE+++K ++
Sbjct: 213 MGVSAVLIENPKGEDWHDQAYNEMQGLKMTCLPLLKESYERAAKLCLEVGQTEQIQKCKN 272

Query: 196 DKNILQKIINFLKLPEDRIISYTKESFYRCVQTIEETLKVHEKGSINIASKKQPLHGQPS 255
             +++QK+ NFL+LP D+I  +TKE FY+ +++IE+  K HE  +  + +K+QPLHGQP 
Sbjct: 273 HMSLMQKMTNFLELPRDKITYFTKEKFYQSMKSIEKFAKAHENRNTFLVNKQQPLHGQPG 332

Query: 256 LCQSHTNPTQQTD---------------SSDSSSPRAPREIGSLRSEADWIQKYTPRNRQ 315
           + QSH NP Q++D               SSDSSSPR P EIGS R EA+ IQK   + RQ
Sbjct: 333 ISQSHINPVQRSDNANPHFQPLNLAATGSSDSSSPRTPSEIGSSRPEANRIQKNLLQKRQ 392

Query: 316 HSEKIEQEFKSSWIKQNQKSTENIQAIRRSGMYLENHFNSKFFPQTH-EASRLSQIAERA 375
             E I+QEF+SSWI+Q QKSTE+IQAI RSG+ L+NH NSK  PQ+H EAS+LS+IAERA
Sbjct: 393 QPEIIKQEFQSSWIQQMQKSTESIQAINRSGVSLQNHLNSKHSPQSHEEASQLSKIAERA 452

Query: 376 LPKIACNSLY---GRASPT-SPSTDGLGKISSNVSSLWSPISQFSRGYSSLKLLNSKSEI 435
           L K  C+S+Y   GRASPT S ST GLGK SSNVS L S   Q+    +S+ LLNSK++I
Sbjct: 453 LSKDPCSSVYGRDGRASPTPSSSTVGLGKSSSNVSCLSSLNFQYPETRNSVNLLNSKTKI 512

Query: 436 EVPSHEIRSYNTMTCRLAETTRFGNNGQLSTTTQPHNRLLKAVESISNEALRAAILEISS 495
           +V SHEIRS    T + AE T  G++  LST TQP NRLLKAV+S+S++ALR AI  ISS
Sbjct: 513 QVKSHEIRSSGISTSQFAEPTSLGSSQHLSTATQPLNRLLKAVDSMSDQALRTAISGISS 572

Query: 496 VTNMEDRITEPWF----C-EKETHLSLQDGGGFSKNMKRKMNATTLNDMPSPCSDIVGSE 555
           V NM D +TEP      C +K  HLSLQDG G S NMKRK+ A TLNDMPSPCSD  GSE
Sbjct: 573 VGNMCDTVTEPSVFGTRCHQKAAHLSLQDGFGSSNNMKRKICAITLNDMPSPCSDNDGSE 632

Query: 556 PTVTSISKKLKKLSDNALLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSYRGA 615
            TVTS SKKLKKL+DNALLEEMRNIN+  +ETVLELD+ +NVNRR ANAGTV+RC+Y   
Sbjct: 633 LTVTSRSKKLKKLTDNALLEEMRNINQRLVETVLELDSAQNVNRRFANAGTVVRCTYSAV 692

Query: 616 TEGKNSVFHSENNTLKLPVLSVKLLVPLDYPEDYPVFLSKLNTDCGTEDGEFRDMWSKAT 675
           ++  N +FH   NTLKLPVLSVKLLVPLDYPEDYPVFLSK NTDCG ED E RD+  KAT
Sbjct: 693 SDRNNLMFHFA-NTLKLPVLSVKLLVPLDYPEDYPVFLSKFNTDCGNEDEECRDLSRKAT 752

Query: 676 SLLSAFLRTSPVYLSLEECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWEDCAA 699
           S+L AFLRT+P  LSL E A+AWD+CAR VV +YAQR GGGCFSSRYG+WEDC A
Sbjct: 753 SMLRAFLRTAPERLSLGEYARAWDQCARYVVSEYAQRTGGGCFSSRYGTWEDCVA 806

BLAST of CmaCh06G000210 vs. ExPASy TrEMBL
Match: A0A6J1I7W8 (mediator of RNA polymerase II transcription subunit 15a-like OS=Cucurbita maxima OX=3661 GN=LOC111469996 PE=4 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 7.0e-189
Identity = 343/375 (91.47%), Postives = 343/375 (91.47%), Query Frame = 0

Query: 325 MYLENHFNSKFFPQTHEASRLSQIAERALPKIACNSLYGRASPTSPSTDGLGKISSNVSS 384
           MYLENHFNSKFFPQTHEASRLSQIAERALPKIACNSLYGRASPTSPSTDGLGKISSNVSS
Sbjct: 1   MYLENHFNSKFFPQTHEASRLSQIAERALPKIACNSLYGRASPTSPSTDGLGKISSNVSS 60

Query: 385 LWSPISQFSRGYSSLKLLNSKSEIEVPSHEIRSYNTMTCRLAETTRFGNNGQLSTTTQPH 444
           LWSPISQFSR                                ETTRFGNNGQLSTTTQPH
Sbjct: 61  LWSPISQFSR--------------------------------ETTRFGNNGQLSTTTQPH 120

Query: 445 NRLLKAVESISNEALRAAILEISSVTNMEDRITEPWFCEKETHLSLQDGGGFSKNMKRKM 504
           NRLLKAVESISNEALRAAILEISSVTNMEDRITEPWFCEKETHLSLQDGGGFSKNMKRKM
Sbjct: 121 NRLLKAVESISNEALRAAILEISSVTNMEDRITEPWFCEKETHLSLQDGGGFSKNMKRKM 180

Query: 505 NATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDNALLEEMRNINRHFIETVLELDTDEN 564
           NATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDNALLEEMRNINRHFIETVLELDTDEN
Sbjct: 181 NATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDNALLEEMRNINRHFIETVLELDTDEN 240

Query: 565 VNRRLANAGTVLRCSYRGATEGKNSVFHSENNTLKLPVLSVKLLVPLDYPEDYPVFLSKL 624
           VNRRLANAGTVLRCSYRGATEGKNSVFHSENNTLKLPVLSVKLLVPLDYPEDYPVFLSKL
Sbjct: 241 VNRRLANAGTVLRCSYRGATEGKNSVFHSENNTLKLPVLSVKLLVPLDYPEDYPVFLSKL 300

Query: 625 NTDCGTEDGEFRDMWSKATSLLSAFLRTSPVYLSLEECAKAWDECARAVVCDYAQRAGGG 684
           NTDCGTEDGEFRDMWSKATSLLSAFLRTSPVYLSLEECAKAWDECARAVVCDYAQRAGGG
Sbjct: 301 NTDCGTEDGEFRDMWSKATSLLSAFLRTSPVYLSLEECAKAWDECARAVVCDYAQRAGGG 343

Query: 685 CFSSRYGSWEDCAAT 700
           CFSSRYGSWEDCAAT
Sbjct: 361 CFSSRYGSWEDCAAT 343

BLAST of CmaCh06G000210 vs. NCBI nr
Match: XP_022971183.1 (mediator of RNA polymerase II transcription subunit 15a isoform X1 [Cucurbita maxima] >XP_022971184.1 mediator of RNA polymerase II transcription subunit 15a isoform X1 [Cucurbita maxima])

HSP 1 Score: 1391.3 bits (3600), Expect = 0.0e+00
Identity = 699/699 (100.00%), Postives = 699/699 (100.00%), Query Frame = 0

Query: 1   MEHEKKLFKNANSEEQHIPQSLSQPHERLHGQKLKVRQTHQQYVMHNQSCVKPQNILTAP 60
           MEHEKKLFKNANSEEQHIPQSLSQPHERLHGQKLKVRQTHQQYVMHNQSCVKPQNILTAP
Sbjct: 1   MEHEKKLFKNANSEEQHIPQSLSQPHERLHGQKLKVRQTHQQYVMHNQSCVKPQNILTAP 60

Query: 61  CRPQGFQSRAFGNHMPPEMFTQHPKVVNLQPNENLSAQVKEEVIDEDFIGSTFLHQHYSA 120
           CRPQGFQSRAFGNHMPPEMFTQHPKVVNLQPNENLSAQVKEEVIDEDFIGSTFLHQHYSA
Sbjct: 61  CRPQGFQSRAFGNHMPPEMFTQHPKVVNLQPNENLSAQVKEEVIDEDFIGSTFLHQHYSA 120

Query: 121 IEQHKQHQSVGASAVPNGNPNAEDWHDLALSEVQRLRTHLPLLKKAYVRASEQCLQVGQT 180
           IEQHKQHQSVGASAVPNGNPNAEDWHDLALSEVQRLRTHLPLLKKAYVRASEQCLQVGQT
Sbjct: 121 IEQHKQHQSVGASAVPNGNPNAEDWHDLALSEVQRLRTHLPLLKKAYVRASEQCLQVGQT 180

Query: 181 EKMEKYEHDKNILQKIINFLKLPEDRIISYTKESFYRCVQTIEETLKVHEKGSINIASKK 240
           EKMEKYEHDKNILQKIINFLKLPEDRIISYTKESFYRCVQTIEETLKVHEKGSINIASKK
Sbjct: 181 EKMEKYEHDKNILQKIINFLKLPEDRIISYTKESFYRCVQTIEETLKVHEKGSINIASKK 240

Query: 241 QPLHGQPSLCQSHTNPTQQTDSSDSSSPRAPREIGSLRSEADWIQKYTPRNRQHSEKIEQ 300
           QPLHGQPSLCQSHTNPTQQTDSSDSSSPRAPREIGSLRSEADWIQKYTPRNRQHSEKIEQ
Sbjct: 241 QPLHGQPSLCQSHTNPTQQTDSSDSSSPRAPREIGSLRSEADWIQKYTPRNRQHSEKIEQ 300

Query: 301 EFKSSWIKQNQKSTENIQAIRRSGMYLENHFNSKFFPQTHEASRLSQIAERALPKIACNS 360
           EFKSSWIKQNQKSTENIQAIRRSGMYLENHFNSKFFPQTHEASRLSQIAERALPKIACNS
Sbjct: 301 EFKSSWIKQNQKSTENIQAIRRSGMYLENHFNSKFFPQTHEASRLSQIAERALPKIACNS 360

Query: 361 LYGRASPTSPSTDGLGKISSNVSSLWSPISQFSRGYSSLKLLNSKSEIEVPSHEIRSYNT 420
           LYGRASPTSPSTDGLGKISSNVSSLWSPISQFSRGYSSLKLLNSKSEIEVPSHEIRSYNT
Sbjct: 361 LYGRASPTSPSTDGLGKISSNVSSLWSPISQFSRGYSSLKLLNSKSEIEVPSHEIRSYNT 420

Query: 421 MTCRLAETTRFGNNGQLSTTTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW 480
           MTCRLAETTRFGNNGQLSTTTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW
Sbjct: 421 MTCRLAETTRFGNNGQLSTTTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW 480

Query: 481 FCEKETHLSLQDGGGFSKNMKRKMNATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDNA 540
           FCEKETHLSLQDGGGFSKNMKRKMNATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDNA
Sbjct: 481 FCEKETHLSLQDGGGFSKNMKRKMNATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDNA 540

Query: 541 LLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSYRGATEGKNSVFHSENNTLKL 600
           LLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSYRGATEGKNSVFHSENNTLKL
Sbjct: 541 LLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSYRGATEGKNSVFHSENNTLKL 600

Query: 601 PVLSVKLLVPLDYPEDYPVFLSKLNTDCGTEDGEFRDMWSKATSLLSAFLRTSPVYLSLE 660
           PVLSVKLLVPLDYPEDYPVFLSKLNTDCGTEDGEFRDMWSKATSLLSAFLRTSPVYLSLE
Sbjct: 601 PVLSVKLLVPLDYPEDYPVFLSKLNTDCGTEDGEFRDMWSKATSLLSAFLRTSPVYLSLE 660

Query: 661 ECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWEDCAAT 700
           ECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWEDCAAT
Sbjct: 661 ECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWEDCAAT 699

BLAST of CmaCh06G000210 vs. NCBI nr
Match: XP_022933464.1 (mediator of RNA polymerase II transcription subunit 15a isoform X3 [Cucurbita moschata])

HSP 1 Score: 1263.4 bits (3268), Expect = 0.0e+00
Identity = 643/699 (91.99%), Postives = 655/699 (93.71%), Query Frame = 0

Query: 1   MEHEKKLFKNANSEEQHIPQSLSQPHERLHGQKLKVRQTHQQYVMHNQSCVKPQNILTAP 60
           MEHEKKLFKNANS+EQHIPQSLSQPHERLHGQKLKVRQTHQQY+MHNQSCV+PQN LTAP
Sbjct: 1   MEHEKKLFKNANSKEQHIPQSLSQPHERLHGQKLKVRQTHQQYLMHNQSCVRPQNTLTAP 60

Query: 61  CRPQGFQSRAFGNHMPPEMFTQHPKVVNLQPNENLSAQVKEEVIDEDFIGSTFLHQHYSA 120
           CRPQGFQSRAFGNHMPPEMFTQHPKVVNLQPNENLSAQVKEEVIDEDFIGSTFLHQ+YSA
Sbjct: 61  CRPQGFQSRAFGNHMPPEMFTQHPKVVNLQPNENLSAQVKEEVIDEDFIGSTFLHQYYSA 120

Query: 121 IEQHKQHQSVGASAVPNGNPNAEDWHDLALSEVQRLRTHLPLLKKAYVRASEQCLQVGQT 180
           IEQHKQHQ+VGASAVPNGNPNAEDWHDLALSEVQRLRTHLPLL KAYVRASEQCLQVGQT
Sbjct: 121 IEQHKQHQTVGASAVPNGNPNAEDWHDLALSEVQRLRTHLPLLTKAYVRASEQCLQVGQT 180

Query: 181 EKMEKYEHDKNILQKIINFLKLPEDRIISYTKESFYRCVQTIEETLKVHEKGSINIASKK 240
           EKM+KYEHDKNILQKIINFLKLPEDRIISYTKESFYRCVQTIEETLKVHE GSINIASK+
Sbjct: 181 EKMQKYEHDKNILQKIINFLKLPEDRIISYTKESFYRCVQTIEETLKVHENGSINIASKR 240

Query: 241 QPLHGQPSLCQSHTNPTQQTDSSDSSSPRAPREIGSLRSEADWIQKYTPRNRQHSEKIEQ 300
           QPLHGQPSLCQSH NP QQT+S DSSSPRAPREIGSLRSEADWIQKYTPRNRQHSEKIEQ
Sbjct: 241 QPLHGQPSLCQSHINPAQQTNSFDSSSPRAPREIGSLRSEADWIQKYTPRNRQHSEKIEQ 300

Query: 301 EFKSSWIKQNQKSTENIQAIRRSGMYLENHFNSKFFPQTHEASRLSQIAERALPKIACNS 360
           EFKSSWIKQNQKSTENIQAIRRSGMYLENHFNSKF PQTHEASRLSQIAERALPKIACNS
Sbjct: 301 EFKSSWIKQNQKSTENIQAIRRSGMYLENHFNSKFSPQTHEASRLSQIAERALPKIACNS 360

Query: 361 LYGRASPTSPSTDGLGKISSNVSSLWSPISQFSRGYSSLKLLNSKSEIEVPSHEIRSYNT 420
           LYGRASPT PSTDGLGKISSNVSSLWSPISQFS+                          
Sbjct: 361 LYGRASPTFPSTDGLGKISSNVSSLWSPISQFSQ-------------------------- 420

Query: 421 MTCRLAETTRFGNNGQLSTTTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW 480
                 ETTRFGNNGQLS TTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW
Sbjct: 421 ------ETTRFGNNGQLS-TTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW 480

Query: 481 FCEKETHLSLQDGGGFSKNMKRKMNATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDNA 540
           FCEKETHLSLQDGGG SKNMKRKMNATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDNA
Sbjct: 481 FCEKETHLSLQDGGGSSKNMKRKMNATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDNA 540

Query: 541 LLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSYRGATEGKNSVFHSENNTLKL 600
           LLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSYR ATEGKNSVFHSENNTLKL
Sbjct: 541 LLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSYRAATEGKNSVFHSENNTLKL 600

Query: 601 PVLSVKLLVPLDYPEDYPVFLSKLNTDCGTEDGEFRDMWSKATSLLSAFLRTSPVYLSLE 660
           PVLSVKLLVPLDYPEDYPVFLSKLNT+CGTED EFRDMWSKATSLL+AFLRT+PVYLSLE
Sbjct: 601 PVLSVKLLVPLDYPEDYPVFLSKLNTECGTEDEEFRDMWSKATSLLNAFLRTAPVYLSLE 660

Query: 661 ECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWEDCAAT 700
           ECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWEDCAAT
Sbjct: 661 ECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWEDCAAT 666

BLAST of CmaCh06G000210 vs. NCBI nr
Match: KAG6596167.1 (Mediator of RNA polymerase II transcription subunit 15a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1256.1 bits (3249), Expect = 0.0e+00
Identity = 640/699 (91.56%), Postives = 652/699 (93.28%), Query Frame = 0

Query: 1   MEHEKKLFKNANSEEQHIPQSLSQPHERLHGQKLKVRQTHQQYVMHNQSCVKPQNILTAP 60
           MEHEKKLFKNANS+EQHIPQSLSQPHERLHGQKLKVRQTH+QYVMHNQSCV+PQN LTAP
Sbjct: 1   MEHEKKLFKNANSKEQHIPQSLSQPHERLHGQKLKVRQTHRQYVMHNQSCVRPQNTLTAP 60

Query: 61  CRPQGFQSRAFGNHMPPEMFTQHPKVVNLQPNENLSAQVKEEVIDEDFIGSTFLHQHYSA 120
           CRPQGFQSRAFGNHMPPEMFTQHPKVVNLQPNENLSAQVKEEV+DEDFIGSTFLHQHYSA
Sbjct: 61  CRPQGFQSRAFGNHMPPEMFTQHPKVVNLQPNENLSAQVKEEVVDEDFIGSTFLHQHYSA 120

Query: 121 IEQHKQHQSVGASAVPNGNPNAEDWHDLALSEVQRLRTHLPLLKKAYVRASEQCLQVGQT 180
           IEQHKQHQSVGASAVPNGNPNAEDWHDLALSEVQRLRTHLPLL KAYVRASEQCLQV QT
Sbjct: 121 IEQHKQHQSVGASAVPNGNPNAEDWHDLALSEVQRLRTHLPLLTKAYVRASEQCLQVAQT 180

Query: 181 EKMEKYEHDKNILQKIINFLKLPEDRIISYTKESFYRCVQTIEETLKVHEKGSINIASKK 240
           EKM+KYEHDKNILQKIINFLKLPEDRIISYTKESFYRCVQTIEETLK HE GSINIASK+
Sbjct: 181 EKMQKYEHDKNILQKIINFLKLPEDRIISYTKESFYRCVQTIEETLKGHENGSINIASKQ 240

Query: 241 QPLHGQPSLCQSHTNPTQQTDSSDSSSPRAPREIGSLRSEADWIQKYTPRNRQHSEKIEQ 300
           QPLHGQPSLCQSH NP QQT+SS+SSSPRAPREIGSLRSEADWIQKYTPRNRQHSEKIEQ
Sbjct: 241 QPLHGQPSLCQSHINPAQQTNSSNSSSPRAPREIGSLRSEADWIQKYTPRNRQHSEKIEQ 300

Query: 301 EFKSSWIKQNQKSTENIQAIRRSGMYLENHFNSKFFPQTHEASRLSQIAERALPKIACNS 360
           EFKSSWIKQNQKSTENIQAIRRSGMYLENHFNSKF PQTHEASRLSQIAERALPKIACNS
Sbjct: 301 EFKSSWIKQNQKSTENIQAIRRSGMYLENHFNSKFSPQTHEASRLSQIAERALPKIACNS 360

Query: 361 LYGRASPTSPSTDGLGKISSNVSSLWSPISQFSRGYSSLKLLNSKSEIEVPSHEIRSYNT 420
           LYGRASPT PSTDGLGKISSNVSSLWSPISQFS+                          
Sbjct: 361 LYGRASPTFPSTDGLGKISSNVSSLWSPISQFSQ-------------------------- 420

Query: 421 MTCRLAETTRFGNNGQLSTTTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW 480
                 ETTRFGNNGQLS TTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW
Sbjct: 421 ------ETTRFGNNGQLS-TTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW 480

Query: 481 FCEKETHLSLQDGGGFSKNMKRKMNATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDNA 540
           FCEKETHLSLQDGGG SKNMKRKMNATTLN MPSPCSDIVGSEPTVTSISKKLKKLSDNA
Sbjct: 481 FCEKETHLSLQDGGGSSKNMKRKMNATTLNGMPSPCSDIVGSEPTVTSISKKLKKLSDNA 540

Query: 541 LLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSYRGATEGKNSVFHSENNTLKL 600
           LLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSYR ATEGKNSVFHSENNTLKL
Sbjct: 541 LLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSYRAATEGKNSVFHSENNTLKL 600

Query: 601 PVLSVKLLVPLDYPEDYPVFLSKLNTDCGTEDGEFRDMWSKATSLLSAFLRTSPVYLSLE 660
           PVLSVKLLVPLDYPEDYPVFLSKLNT+CGTED EFRDMWSKATSLL+A LRT+PVYLSLE
Sbjct: 601 PVLSVKLLVPLDYPEDYPVFLSKLNTECGTEDEEFRDMWSKATSLLNALLRTAPVYLSLE 660

Query: 661 ECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWEDCAAT 700
           ECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWEDCAAT
Sbjct: 661 ECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWEDCAAT 666

BLAST of CmaCh06G000210 vs. NCBI nr
Match: KAG7027708.1 (Mediator of RNA polymerase II transcription subunit 15a [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1183.3 bits (3060), Expect = 0.0e+00
Identity = 613/699 (87.70%), Postives = 624/699 (89.27%), Query Frame = 0

Query: 1   MEHEKKLFKNANSEEQHIPQSLSQPHERLHGQKLKVRQTHQQYVMHNQSCVKPQNILTAP 60
           MEHEKKLFKNANS+EQHIPQSLSQPHERLHGQKLK                         
Sbjct: 1   MEHEKKLFKNANSKEQHIPQSLSQPHERLHGQKLK------------------------- 60

Query: 61  CRPQGFQSRAFGNHMPPEMFTQHPKVVNLQPNENLSAQVKEEVIDEDFIGSTFLHQHYSA 120
               GFQSRAFGNHMPPEMFTQHPKVVNLQPNENLSAQVKEEV+DEDFIGSTFLHQHYSA
Sbjct: 61  ----GFQSRAFGNHMPPEMFTQHPKVVNLQPNENLSAQVKEEVVDEDFIGSTFLHQHYSA 120

Query: 121 IEQHKQHQSVGASAVPNGNPNAEDWHDLALSEVQRLRTHLPLLKKAYVRASEQCLQVGQT 180
           IEQHKQHQSVGASAVPNGNPNAEDWHDLALSEVQRLRTHLPLL KAYVRASEQCLQV +T
Sbjct: 121 IEQHKQHQSVGASAVPNGNPNAEDWHDLALSEVQRLRTHLPLLTKAYVRASEQCLQVAET 180

Query: 181 EKMEKYEHDKNILQKIINFLKLPEDRIISYTKESFYRCVQTIEETLKVHEKGSINIASKK 240
           EKM+KYEHDKNILQKIINFLKLPEDRIISYTKESFYRCVQTIEETLK HE GSINIASK+
Sbjct: 181 EKMQKYEHDKNILQKIINFLKLPEDRIISYTKESFYRCVQTIEETLKGHENGSINIASKQ 240

Query: 241 QPLHGQPSLCQSHTNPTQQTDSSDSSSPRAPREIGSLRSEADWIQKYTPRNRQHSEKIEQ 300
           QPLHGQPSLCQSH NP QQT+SS+SSSPRAPREIGSLRSEADWIQKYTPRNRQHSEKIEQ
Sbjct: 241 QPLHGQPSLCQSHINPAQQTNSSNSSSPRAPREIGSLRSEADWIQKYTPRNRQHSEKIEQ 300

Query: 301 EFKSSWIKQNQKSTENIQAIRRSGMYLENHFNSKFFPQTHEASRLSQIAERALPKIACNS 360
           EFKSSWIKQNQKSTENIQAIRRSGMYLENHFNSKF PQTHEASRLSQIAERALPKIACNS
Sbjct: 301 EFKSSWIKQNQKSTENIQAIRRSGMYLENHFNSKFSPQTHEASRLSQIAERALPKIACNS 360

Query: 361 LYGRASPTSPSTDGLGKISSNVSSLWSPISQFSRGYSSLKLLNSKSEIEVPSHEIRSYNT 420
           LYGRASPT PSTDGLGKISSNVSSLWSPISQFS+                          
Sbjct: 361 LYGRASPTFPSTDGLGKISSNVSSLWSPISQFSQ-------------------------- 420

Query: 421 MTCRLAETTRFGNNGQLSTTTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW 480
                 ETTRFGNNGQLS TTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW
Sbjct: 421 ------ETTRFGNNGQLS-TTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW 480

Query: 481 FCEKETHLSLQDGGGFSKNMKRKMNATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDNA 540
           FCEKETHLSLQDGGG SKNMKRKMNATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDNA
Sbjct: 481 FCEKETHLSLQDGGGSSKNMKRKMNATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDNA 540

Query: 541 LLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSYRGATEGKNSVFHSENNTLKL 600
           LLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSYR ATEGKNSV HSENNTLKL
Sbjct: 541 LLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSYRAATEGKNSVIHSENNTLKL 600

Query: 601 PVLSVKLLVPLDYPEDYPVFLSKLNTDCGTEDGEFRDMWSKATSLLSAFLRTSPVYLSLE 660
           PVLSVKLLVPLDYPEDYPVFLSKLNT+CGTED EFRDMWSKATSLL+A LRT+PVYLSLE
Sbjct: 601 PVLSVKLLVPLDYPEDYPVFLSKLNTECGTEDEEFRDMWSKATSLLNALLRTAPVYLSLE 637

Query: 661 ECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWEDCAAT 700
           ECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWEDCAAT
Sbjct: 661 ECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWEDCAAT 637

BLAST of CmaCh06G000210 vs. NCBI nr
Match: XP_023539395.1 (uncharacterized protein LOC111800051 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1137.5 bits (2941), Expect = 0.0e+00
Identity = 583/631 (92.39%), Postives = 589/631 (93.34%), Query Frame = 0

Query: 1   MEHEKKLFKNANSEEQHIPQSLSQPHERLHGQKLKVRQTHQQYVMHNQSCVKPQNILTAP 60
           MEHEKKLFKNANS+EQHIPQSLSQPHERLHGQKLKVRQTHQQYVMHNQSCV+PQN LTAP
Sbjct: 1   MEHEKKLFKNANSKEQHIPQSLSQPHERLHGQKLKVRQTHQQYVMHNQSCVRPQNTLTAP 60

Query: 61  CRPQGFQSRAFGNHMPPEMFTQHPKVVNLQPNENLSAQVKEEVIDEDFIGSTFLHQHYSA 120
           CRPQGFQSRAFGNHMPPEMFTQHPKVVNLQPNENLSAQVKEEVIDEDFIGSTFLHQHYSA
Sbjct: 61  CRPQGFQSRAFGNHMPPEMFTQHPKVVNLQPNENLSAQVKEEVIDEDFIGSTFLHQHYSA 120

Query: 121 IEQHKQHQSVGASAVPNGNPNAEDWHDLALSEVQRLRTHLPLLKKAYVRASEQCLQVGQT 180
           IEQHKQHQSVGASAVPNGNPNAEDWHDLALSEVQRLRTHLPLLKKAYVRASEQCLQVGQT
Sbjct: 121 IEQHKQHQSVGASAVPNGNPNAEDWHDLALSEVQRLRTHLPLLKKAYVRASEQCLQVGQT 180

Query: 181 EKMEKYEHDKNILQKIINFLKLPEDRIISYTKESFYRCVQTIEETLKVHEKGSINIASKK 240
           EKM+KYEHDKNILQKIINFLKLPEDRIISYTKESFYRCVQTIEETLKVHE GSINIASK+
Sbjct: 181 EKMQKYEHDKNILQKIINFLKLPEDRIISYTKESFYRCVQTIEETLKVHENGSINIASKQ 240

Query: 241 QPLHGQPSLCQSHTNPTQQTDSSDSSSPRAPREIGSLRSEADWIQKYTPRNRQHSEKIEQ 300
           Q LHGQPSLCQSH NP QQTDSSDSSSPRAPREIGSLRSEADWIQKYTPRNRQHSEKIEQ
Sbjct: 241 QALHGQPSLCQSHINPAQQTDSSDSSSPRAPREIGSLRSEADWIQKYTPRNRQHSEKIEQ 300

Query: 301 EFKSSWIKQNQKSTENIQAIRRSGMYLENHFNSKFFPQTHEASRLSQIAERALPKIACNS 360
           EFKSSWIKQNQKSTENIQAIRRSGMYLENHFNSKF PQTHEASRLSQIAERALPKIACNS
Sbjct: 301 EFKSSWIKQNQKSTENIQAIRRSGMYLENHFNSKFSPQTHEASRLSQIAERALPKIACNS 360

Query: 361 LYGRASPTSPSTDGLGKISSNVSSLWSPISQFSRGYSSLKLLNSKSEIEVPSHEIRSYNT 420
           LYGRASPTSPSTDGLGKISSNVSSLWSPISQFS+                          
Sbjct: 361 LYGRASPTSPSTDGLGKISSNVSSLWSPISQFSQ-------------------------- 420

Query: 421 MTCRLAETTRFGNNGQLSTTTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW 480
                 ETTRFGNNGQLSTTTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW
Sbjct: 421 ------ETTRFGNNGQLSTTTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW 480

Query: 481 FCEKETHLSLQDGGGFSKNMKRKMNATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDNA 540
           FCEKETHLSLQDGGG SKNMKRKMNATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDNA
Sbjct: 481 FCEKETHLSLQDGGGSSKNMKRKMNATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDNA 540

Query: 541 LLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSYRGATEGKNSVFHSENNTLKL 600
           LLEEMRNINRHFIETVLELDTDE VNRRLANAGTVLRCSYR ATEGKNSVFHSENNTLKL
Sbjct: 541 LLEEMRNINRHFIETVLELDTDEIVNRRLANAGTVLRCSYRAATEGKNSVFHSENNTLKL 599

Query: 601 PVLSVKLLVPLDYPEDYPVFLSKLNTDCGTE 632
           PVLSVKLLVPLDYPEDYPVFLSKLNTDCG +
Sbjct: 601 PVLSVKLLVPLDYPEDYPVFLSKLNTDCGNQ 599

BLAST of CmaCh06G000210 vs. TAIR 10
Match: AT1G15780.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G10440.1); Has 103701 Blast hits to 43153 proteins in 1828 species: Archae - 30; Bacteria - 7385; Metazoa - 38639; Fungi - 11531; Plants - 7727; Viruses - 307; Other Eukaryotes - 38082 (source: NCBI BLink). )

HSP 1 Score: 137.1 bits (344), Expect = 5.4e-32
Identity = 102/301 (33.89%), Postives = 150/301 (49.83%), Query Frame = 0

Query: 434  NGQLSTTTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRIT-------------EPW 493
            +G+ S T  P  RL++AV+SIS +AL +A+ +I SV +M DRI              E  
Sbjct: 1037 SGKPSATELPIERLIRAVKSISPQALSSAVSDIGSVVSMVDRIAGSAPGNGSRASVGEDL 1096

Query: 494  FCEKETHLS-----LQDGGGFSKNMKRKMNATTL-----------NDMPSPCSDIVGSEP 553
                +  L       Q+G   +K MKR   A  L           N      S+    E 
Sbjct: 1097 VAMTKCRLQARNFMTQEGMMATKKMKRHTTAMPLSVASLGGSVGDNYKQFAGSETSDLES 1156

Query: 554  TVTSISKKLKKLSDNALLEEMRNINRHFIETVLELDTDENVN-------RRLANAGTVLR 613
            T TS  KK +  +++ALLEE++ IN+  I+TV+E+  DE+           +   GT +R
Sbjct: 1157 TATSDGKKARTETEHALLEEIKEINQRLIDTVVEISDDEDAADPSEVAISSIGCEGTTVR 1216

Query: 614  CSYRGATEGKNSVFHSENNTLKLPVLSVKLLVPLDYPEDYPVFLSKLNTDCGTEDGEFRD 673
             S+   +       H  ++T   P+  ++LLVP  YP   P  L KL  +   E+    D
Sbjct: 1217 FSFIAVSLSPALKAHL-SSTQMSPIQPLRLLVPCSYPNGSPSLLDKLPVETSKEN---ED 1276

Query: 674  MWSKATSLLSAFLRTSPVYLSLEECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWEDCA 699
            + SKA +  +  LR+    +SL++ AK WD CARAV+C+YAQ+ GGG FSS+YG+WE   
Sbjct: 1277 LSSKAMARFNILLRSLSQPMSLKDIAKTWDACARAVICEYAQQFGGGTFSSKYGTWEKYV 1333

BLAST of CmaCh06G000210 vs. TAIR 10
Match: AT2G10440.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G15780.1); Has 1628 Blast hits to 1350 proteins in 149 species: Archae - 0; Bacteria - 39; Metazoa - 480; Fungi - 159; Plants - 187; Viruses - 2; Other Eukaryotes - 761 (source: NCBI BLink). )

HSP 1 Score: 98.2 bits (243), Expect = 2.8e-20
Identity = 111/457 (24.29%), Postives = 205/457 (44.86%), Query Frame = 0

Query: 271 PREIGSLRSEADWIQKYTPRNRQHSEKIEQEFKSSWIKQNQKSTENIQAIRR------SG 330
           PR + +  S    +QK   + R H  +++Q+       Q+Q  T  +  +R         
Sbjct: 407 PRAVNTNISSVQSLQK---QKRFHHRQMQQQQPQQGNHQHQMQTNEMNDVRMRERVNIKA 466

Query: 331 MYLENHFNS--KFFPQTHEASRLSQIAERALPKIACNSLYGRASPTSPSTDGLGKISSNV 390
             LE   +S  +  P+       SQI   + P++    +     P + +  G   ++S+ 
Sbjct: 467 RLLEQQVSSSQRQVPKQESNVSSSQIQNHSSPQLVDQHIL----PATINKTGT-PLNSSG 526

Query: 391 SSLWSPISQFSRGYSSLKLLNSKSEIEVP---SHEIRSYNTMTCRLA--ETTRFGNNGQL 450
           S+  +P      G S + +      +E P     EI S    + +L   ET         
Sbjct: 527 SAFVAPAPSPVPGDSEMPI-----SVESPVSGVDEINSTLDSSSKLGTQETPLLFVPPPE 586

Query: 451 STTTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW-------------FCEK 510
             T +P +RL+KA ++ S ++L  ++ EISSV +M D I   +               E+
Sbjct: 587 PITERPIDRLIKAFQAASPKSLAESVSEISSVISMVDMIGGSFPSSGGSRAGLGEDLSER 646

Query: 511 ETHLSLQDGGGFSKNMKRKMN-----ATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDN 570
             + +  +    SK MKR +N      ++  D     S +     + TS   K+  ++  
Sbjct: 647 TRNFTTHEETNLSKRMKRSINIVPPDMSSQIDSYEQLSSLESEVVSTTSSGLKVNNIAPG 706

Query: 571 -ALLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSYRGATEGKNSVFHSENNTL 630
            ALL+E++  N   +ETV+E+  ++++       GT++ C+Y  A    ++ F     + 
Sbjct: 707 YALLQEIKETNGRLVETVVEICDEDSL-------GTIVTCTY--APVALSATFKDHYKSG 766

Query: 631 KL-PVLSVKLLVPLDYPEDYPVFLSKLNTDCGTEDGEFRDMWSKATSLLSAFLRTSPVYL 690
           K+  +  ++LL P+DYP   P+ L +++ D  T   ++ D+ ++  S  S  ++      
Sbjct: 767 KIAQIQPLRLLFPMDYPYSSPIVLEEISFD--TSVHKYEDLSARTRSRFSLSMKEFSEPG 826

Query: 691 SLEECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWE 695
             +  A+ W++CARA + +YA+R GGG FSS+YG+WE
Sbjct: 827 FSKGIAQTWNDCARATMVEYAERHGGGTFSSKYGAWE 839

BLAST of CmaCh06G000210 vs. TAIR 10
Match: AT2G10440.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G15780.1); Has 8319 Blast hits to 5104 proteins in 317 species: Archae - 0; Bacteria - 285; Metazoa - 1706; Fungi - 535; Plants - 320; Viruses - 18; Other Eukaryotes - 5455 (source: NCBI BLink). )

HSP 1 Score: 97.4 bits (241), Expect = 4.7e-20
Identity = 109/465 (23.44%), Postives = 205/465 (44.09%), Query Frame = 0

Query: 271 PREIGSLRSEADWIQKYTPRNRQHSEKIEQEFKSSWIKQNQKSTENIQAIRR------SG 330
           PR + +  S    +QK   + R H  +++Q+       Q+Q  T  +  +R         
Sbjct: 487 PRAVNTNISSVQSLQK---QKRFHHRQMQQQQPQQGNHQHQMQTNEMNDVRMRERVNIKA 546

Query: 331 MYLENHFNS--KFFPQTHEASRLSQIAERALPKIACNSLYGRASPTSPSTDGLGKISSNV 390
             LE   +S  +  P+       SQI   + P++    +     P + +  G   ++S+ 
Sbjct: 547 RLLEQQVSSSQRQVPKQESNVSSSQIQNHSSPQLVDQHIL----PATINKTGT-PLNSSG 606

Query: 391 SSLWSPISQFSRGYSSLKLLNSKSEIEVP---SHEIRSYNTMTCRLA--ETTRFGNNGQL 450
           S+  +P      G S + +      +E P     EI S    + +L   ET         
Sbjct: 607 SAFVAPAPSPVPGDSEMPI-----SVESPVSGVDEINSTLDSSSKLGTQETPLLFVPPPE 666

Query: 451 STTTQPHNRLLKAVESISNEALRAAILEISSVTNMEDRITEPW-------------FCEK 510
             T +P +RL+KA ++ S ++L  ++ EISSV +M D I   +               E+
Sbjct: 667 PITERPIDRLIKAFQAASPKSLAESVSEISSVISMVDMIGGSFPSSGGSRAGLGEDLSER 726

Query: 511 ETHLSLQDGGGFSKNMKRKMN-----ATTLNDMPSPCSDIVGSEPTVTSISKKLKKLSDN 570
             + +  +    SK MKR +N      ++  D     S +     + TS   K+  ++  
Sbjct: 727 TRNFTTHEETNLSKRMKRSINIVPPDMSSQIDSYEQLSSLESEVVSTTSSGLKVNNIAPG 786

Query: 571 -ALLEEMRNINRHFIETVLELDTDENVNRRLANAGTVLRCSY---------RGATEGKNS 630
            ALL+E++  N   +ETV+E+  ++++       GT++ C+Y         +   +    
Sbjct: 787 YALLQEIKETNGRLVETVVEICDEDSL-------GTIVTCTYAPVALSATFKDHYKSGKI 846

Query: 631 VFHSENNTLKLPVLSVKLLVPLDYPEDYPVFLSKLNTDCGTEDGEFRDMWSKATSLLSAF 690
           +F+     ++  +  ++LL P+DYP   P+ L +++ D  T   ++ D+ ++  S  S  
Sbjct: 847 IFYVSKCLMQAQIQPLRLLFPMDYPYSSPIVLEEISFD--TSVHKYEDLSARTRSRFSLS 906

Query: 691 LRTSPVYLSLEECAKAWDECARAVVCDYAQRAGGGCFSSRYGSWE 695
           ++        +  A+ W++CARA + +YA+R GGG FSS+YG+WE
Sbjct: 907 MKEFSEPGFSKGIAQTWNDCARATMVEYAERHGGGTFSSKYGAWE 929