CmaCh04G001200 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh04G001200
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	staphylococcal-like nuclease CAN2
Location	Cma_Chr04: 576310 .. 580018 (-)
RNA-Seq Expression	CmaCh04G001200
Synteny	CmaCh04G001200

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTCATTACCTTAGATTCGCCATTGTTTAAGGCAGCAAAAAATGGGGAACGCTCTCAGGTTCCTCTGCAGTCACTTCTGCAATCCTAACGCTGCCGCCGACGCCGATTCCCTCGGTCCTCATGGCGTCTCCCCGGCCACCGTCGGCGTCTCGGCTCTTGCTCACGATCTCTTTGATTTTGAGATCACCTCGCAGGTATGTTCTCAATCTGAAGCTCATATCGAGCTGCATTTCTGGTTGATTGAATTTTGAATCGGATTCTGTCTGGTGATCTAGTGAAGTTTGTTGTTCAAACAATCTATGCTTTGGTATTGATGATTTTAATTTGATGAATTTTTGAAAGGTTCCTCAAGGACTTAGTAAGCATGTGGTTTCGTCGAAGAAGGCACAGACGAATTGGTAAAGTATTTCTGATTGTTTGGTTACGGAGTGGAGATTTATGAAAGTAATTGTAGTTACAGAGTGGTGAGATTCTGATTGTTTGTGAGATCTGTGTGTGTGGGAAGGTATAGAAAACTGGCTGAAGCTTGGAGGTCGGAGAAACCACCTCCTAAAACGCCTGAAGAAGCATCCAGGCTTGTGATTCAGACACTCAAGAGACACCAGAAGAAAGATGTTGAGGTACAAATAGGAATTCTCTTGCTTGTTTTGTTGGATTGGTAAACTGGGAATTTCTGCAGGTTAAATAGATCAATTGATAAGAGTCAAAGTTCATTCTGTCAAATATGAGCAGCTCTGATGATAATAAGGAAATGCGTTGTTTTTTCCATTTCGAATTCAAATTTTTAGGAGAGAGAAATCGGATCCATGGGGACTTAGCTAGATGGCTTGTGTAGAACAATCCAAAATCCAGTGGGGTTGGATCTGATGAACCGAAATGGAAAACAGATTACTTTTTGAAAAGTGTCAAATTACTTTTTGAAAATAGTGTTTTCAAGAACGTTTATGTTTTTAAAAAATAGTTAAATGCTTCCTTTAAAAGCATTTAAGTACTTTCTGTTCATTTTTTAAACTTCTCAACTACTTCTGTTCTTGCAAAATCTATACATATGCCTTTAATTAAAATAATTAAATTGAACTAAAGCTAGAAGTTATGGAAGAAAATAAAAAGAATACAACAACAATTTTAGCCCTCCATAAGCATTTTAAAAGGCATGTCAAACACACTGAATCAAGTTCTGTGTTTTATTTCTGTTGTTCAACAGGGATTATTGGCTTTCTATGGTCTCCCTCTTCCTCATACTCTTGTCGAACTTTCTGCTCCGGCCCCATTGCCTACATCGCTACCAGATGGAGTCAAGTTTGAGCTGCAGACATTGCCAGTAAGTGAGAAGGATGCTTTTATGGGAAATGACCAACCATTTTGTTCACAACAGGTGTTCTCATTTCTCAATGTTGAATTCATATCAGGTCGATGCAAAAGCTGTAGCAGATGGTGATACAGTGACAGTGTATGTGAGTACAGCACACCCTAGAGAGTCCTCTTGTATTCCAATGGAGGTTAGGAACGCAGCTATTCAAAGATCACAAGCTAGAAACGCCAAGAACTATGCAAAGGCTGATGCACTTCACAAGACAATAACTGATGCTGGTTACAGGTAAATTATATCTCTGCTCTACTGCATACATACACTTGGACTTTTTCAGTAAGTTACTGGGATTTGATGCCTGTTTCTTCTCCCTCAAAAGGGTGCTTACTGTTCAGAATGAGGAGATTCTTGCAAGAAAATACAGGATTCGTCTCAGGTAATTAAAATTAGCACCGGGTGATTTGATTCTTGAACTTTTAGTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTCATTTTGATGTATACCTTTTCAAATCGTTTGTCTATTTTGATTCATGTAATTTCAAAATGTCTTCTTTCATTTTTGTACTATTGAAAAGTTCACTGATTTATATTTTCTCTTTTTTTTTTTTTTTTTTATCGCAATTTCACAGCAACATCATTCCGAATTTACTAAAGATACTCTCATAACTTTTATTAAATGATGATCATTGTGCATAGAATCATTTTGCAAATAGACTAAAGATTCATTTTTCAAGAGCACAAAATCAAAATACGATTTAAACCTGAATAAAAATAATGATTGTTCCATACTTTGGAACGATTTCCAAATTCCATTGACCCTTGGGCTTAGTTGAACGTTGATAATGCAGAGGAATAGATGCACCAGAAAGCGCAATGCCTTATGGGAAAGAGGCAAAAGAAGAGCTGAGAAAGCTTGTCGAAGGCAAGTGTTTGAGAGTACTTGTGTACGGAGAAGATCGGTATAACCGATGTGTGGGTGACTTGTATTGTAATGGAAAGTTTATTCAGGTAACACTAATTACACTATTTCCACTCTCCACAATGGATCATATGGCTTCATGTTTCTGGTTATGTAAGAATAAGTTCTTGGTTCTCTCTACAGGAAGCAATGCTGAAAAAGGGCTTTGCGTGGCATTACACTGCCTATGACAAACGCGCAGAGCTGGCAAAGGTGCGTTACATTTTGATTGCATTCGTTAAACTTATATCCCCAAAACTTCAAGTAAAAGTCAGGTATGATAAGAGGACTAGTTTAATTCTTGAAAATTGAGCTTAGTTTTAATTTCTGTTTGGTCCCTAAAATTTGAAATGATCAACGTGATAGTTAATATATACTACTAACTTGCCTAACTTGCCATGTATGTATTTTTTAAATGAACTAAATATATATTATGTTTAATACAAGGACCAATTATAAATCAATCTCACATCCACCTCAACATTTTGGAAAAAAAAAGAAAAAAAACTACAATTCAGACAGATATTTTCACATCTAAGTTTACGAACAGTTTTAATAGATGGAAGCTGTTACGTGTATGCTACGTTACATGTTCGCTTTACTCGATTCATTCTAATTCATGATTCATAACAGTGGGAGAATGAAGCTCGAGCTAGAAGGATAGGGTTGTGGGCTTCTAGAAACCCAGAGCAGCCATGGGAATGGAGAAAGGGAAAGCGTGAAGGGAAATAGAAGCCTTGCCGGCTGCCCTGCTACAATACAAAGCCTTTAACTTATACACAATTTATTTCAAGTCTCTACCAAGAAGAAGAGGAAGCGGACCCAAAACCAACCCGAACAAGGTTTCTTCTGTCTTTAGTATTTATCAACATCTTCAACATTGCTTGTAAATTTGTAACAGCAAATTTGACAAGAAATAGATTATTGTTATACTTTGTAAGACCATATCATTCACTTTTTAAGTTTAATCAGCCAGTACAGCACGAGCATGAGGGAAGGTAGAATACAATAGATAACC

mRNA sequence

ATTTCATTACCTTAGATTCGCCATTGTTTAAGGCAGCAAAAAATGGGGAACGCTCTCAGGTTCCTCTGCAGTCACTTCTGCAATCCTAACGCTGCCGCCGACGCCGATTCCCTCGGTCCTCATGGCGTCTCCCCGGCCACCGTCGGCGTCTCGGCTCTTGCTCACGATCTCTTTGATTTTGAGATCACCTCGCAGGTTCCTCAAGGACTTAGTAAGCATGTGGTTTCGTCGAAGAAGGCACAGACGAATTGGTATAGAAAACTGGCTGAAGCTTGGAGGTCGGAGAAACCACCTCCTAAAACGCCTGAAGAAGCATCCAGGCTTGTGATTCAGACACTCAAGAGACACCAGAAGAAAGATGTTGAGGGATTATTGGCTTTCTATGGTCTCCCTCTTCCTCATACTCTTGTCGAACTTTCTGCTCCGGCCCCATTGCCTACATCGCTACCAGATGGAGTCAAGTTTGAGCTGCAGACATTGCCAGTCGATGCAAAAGCTGTAGCAGATGGTGATACAGTGACAGTGTATGTGAGTACAGCACACCCTAGAGAGTCCTCTTGTATTCCAATGGAGGTTAGGAACGCAGCTATTCAAAGATCACAAGCTAGAAACGCCAAGAACTATGCAAAGGCTGATGCACTTCACAAGACAATAACTGATGCTGGTTACAGGGTGCTTACTGTTCAGAATGAGGAGATTCTTGCAAGAAAATACAGGATTCGTCTCAGAGGAATAGATGCACCAGAAAGCGCAATGCCTTATGGGAAAGAGGCAAAAGAAGAGCTGAGAAAGCTTGTCGAAGGCAAGTGTTTGAGAGTACTTGTGTACGGAGAAGATCGGTATAACCGATGTGTGGGTGACTTGTATTGTAATGGAAAGTTTATTCAGGAAGCAATGCTGAAAAAGGGCTTTGCGTGGCATTACACTGCCTATGACAAACGCGCAGAGCTGGCAAAGTGGGAGAATGAAGCTCGAGCTAGAAGGATAGGGTTGTGGGCTTCTAGAAACCCAGAGCAGCCATGGGAATGGAGAAAGGGAAAGCGTGAAGGGAAATAGAAGCCTTGCCGGCTGCCCTGCTACAATACAAAGCCTTTAACTTATACACAATTTATTTCAAGTCTCTACCAAGAAGAAGAGGAAGCGGACCCAAAACCAACCCGAACAAGGTTTCTTCTGTCTTTAGTATTTATCAACATCTTCAACATTGCTTGTAAATTTGTAACAGCAAATTTGACAAGAAATAGATTATTGTTATACTTTGTAAGACCATATCATTCACTTTTTAAGTTTAATCAGCCAGTACAGCACGAGCATGAGGGAAGGTAGAATACAATAGATAACC

Coding sequence (CDS)

ATGGGGAACGCTCTCAGGTTCCTCTGCAGTCACTTCTGCAATCCTAACGCTGCCGCCGACGCCGATTCCCTCGGTCCTCATGGCGTCTCCCCGGCCACCGTCGGCGTCTCGGCTCTTGCTCACGATCTCTTTGATTTTGAGATCACCTCGCAGGTTCCTCAAGGACTTAGTAAGCATGTGGTTTCGTCGAAGAAGGCACAGACGAATTGGTATAGAAAACTGGCTGAAGCTTGGAGGTCGGAGAAACCACCTCCTAAAACGCCTGAAGAAGCATCCAGGCTTGTGATTCAGACACTCAAGAGACACCAGAAGAAAGATGTTGAGGGATTATTGGCTTTCTATGGTCTCCCTCTTCCTCATACTCTTGTCGAACTTTCTGCTCCGGCCCCATTGCCTACATCGCTACCAGATGGAGTCAAGTTTGAGCTGCAGACATTGCCAGTCGATGCAAAAGCTGTAGCAGATGGTGATACAGTGACAGTGTATGTGAGTACAGCACACCCTAGAGAGTCCTCTTGTATTCCAATGGAGGTTAGGAACGCAGCTATTCAAAGATCACAAGCTAGAAACGCCAAGAACTATGCAAAGGCTGATGCACTTCACAAGACAATAACTGATGCTGGTTACAGGGTGCTTACTGTTCAGAATGAGGAGATTCTTGCAAGAAAATACAGGATTCGTCTCAGAGGAATAGATGCACCAGAAAGCGCAATGCCTTATGGGAAAGAGGCAAAAGAAGAGCTGAGAAAGCTTGTCGAAGGCAAGTGTTTGAGAGTACTTGTGTACGGAGAAGATCGGTATAACCGATGTGTGGGTGACTTGTATTGTAATGGAAAGTTTATTCAGGAAGCAATGCTGAAAAAGGGCTTTGCGTGGCATTACACTGCCTATGACAAACGCGCAGAGCTGGCAAAGTGGGAGAATGAAGCTCGAGCTAGAAGGATAGGGTTGTGGGCTTCTAGAAACCCAGAGCAGCCATGGGAATGGAGAAAGGGAAAGCGTGAAGGGAAATAG

Protein sequence

MGNALRFLCSHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHVVSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPHTLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRNAAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPYGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRAELAKWENEARARRIGLWASRNPEQPWEWRKGKREGK

Homology

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BLAST of CmaCh04G001200 vs. ExPASy Swiss-Prot
Match: F4IH31 (Staphylococcal-like nuclease CAN2 OS=Arabidopsis thaliana OX=3702 GN=CAN2 PE=1 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 4.8e-132
Identity = 234/334 (70.06%), Postives = 271/334 (81.14%), Query Frame = 0


Query: 1   MGNALRFLCSHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGNAL FL    C P      DSLGPHGVS ATVGVSALAHDLF+FEITSQVP+GL ++V
Sbjct: 1   MGNALTFLYGKCCKPTTT--DDSLGPHGVSAATVGVSALAHDLFNFEITSQVPEGLGRYV 60

Query: 61  VSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
            SS+KAQ NWYRK+ EAW+  KPPP+T EEASRLV   LKR+QK DVEGLL+FYGLPL H
Sbjct: 61  QSSRKAQANWYRKILEAWKQAKPPPQTAEEASRLVTDILKRNQKADVEGLLSFYGLPLSH 120

Query: 121 TLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
           TLVE++  AP+  SLP+G+ FE QTLPVD KAVADGDT+TVYVST+ P  SS +P EV  
Sbjct: 121 TLVEVTVEAPV--SLPEGILFEFQTLPVDPKAVADGDTITVYVSTSEPVVSSSVPREVNL 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AA+QR++AR  +NY KAD LH+ I D+GYRVL ++NEE+LARK+RIRLRGIDAPES MP+
Sbjct: 181 AAVQRAKAREKRNYPKADELHQKIIDSGYRVLNIENEEVLARKFRIRLRGIDAPESQMPF 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEA+E L K+V  K L+VLVYGED+Y RCVGDLYCNG F+QEAMLKKG AWHY AYDKR
Sbjct: 241 GKEAQEGLLKIVGRKSLKVLVYGEDQYGRCVGDLYCNGIFVQEAMLKKGLAWHYLAYDKR 300

Query: 301 AELAKWENEARARRIGLWASRNPEQPWEWRKGKR 335
             LAKWE EAR +RIGLWAS NPE+PW+WRK  R
Sbjct: 301 PVLAKWEKEARQKRIGLWASSNPEKPWDWRKNNR 330

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BLAST of CmaCh04G001200 vs. ExPASy Swiss-Prot
Match: Q39635 (Uncharacterized 38.1 kDa protein OS=Capnoides sempervirens OX=3464 PE=2 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 1.8e-126
Identity = 228/338 (67.46%), Postives = 265/338 (78.40%), Query Frame = 0


Query: 1   MGNALRFLCSHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGNALRFL  H C P           +GVS ATVGVSALAHDLF FE TSQ+P+GL+K+V
Sbjct: 1   MGNALRFLYGHCCKPTVEDHYQPPHGYGVSTATVGVSALAHDLFHFENTSQIPEGLTKYV 60

Query: 61  VSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSSKKAQTNWY+KL++AWR  KPPP+T E+A+RL+I TLKRHQK DV+GLL FYGLPL +
Sbjct: 61  VSSKKAQTNWYKKLSQAWREAKPPPQTAEQATRLIILTLKRHQKADVKGLLRFYGLPLSN 120

Query: 121 TLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
                +     P     GVKFEL TLPVD KAVADGDTVTVYV+T  PRE+S +P  V+ 
Sbjct: 121 NPSTEATTVVAPPQADQGVKFELHTLPVDVKAVADGDTVTVYVNTEDPREASNLPKSVKV 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLT-VQNEEILARKYRIRLRGIDAPESAMP 240
           AA +R++AR  ++Y KADAL K I DAGYRVL+   NE+ILARKYRIRLRGID+PES+MP
Sbjct: 181 AAQERAKARAVRDYVKADALQKNIVDAGYRVLSGPNNEDILARKYRIRLRGIDSPESSMP 240

Query: 241 YGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300
           +GKEAKEEL KLV GKCL V +Y EDRY R VGD+YCNG+FIQE MLKKG AWHYTAYDK
Sbjct: 241 FGKEAKEELIKLVVGKCLTVHIYEEDRYGRSVGDIYCNGQFIQEKMLKKGLAWHYTAYDK 300

Query: 301 RAELAKWENEARARRIGLWASRNPEQPWEWRKGKREGK 338
           R +L+KWE +ARA R+GLWAS NPE+PWEWRK KR GK
Sbjct: 301 RPQLSKWEEKARAARVGLWASSNPEKPWEWRKNKRNGK 338

[Close]

BLAST of CmaCh04G001200 vs. ExPASy Swiss-Prot
Match: F4IZC5 (Staphylococcal-like nuclease CAN1 OS=Arabidopsis thaliana OX=3702 GN=CAN1 PE=1 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 2.3e-126
Identity = 225/336 (66.96%), Postives = 263/336 (78.27%), Query Frame = 0


Query: 1   MGNALRFLCSHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGNA+R L               L  HGVS ++ GVSAL+ DL +FE TSQVP+ L  +V
Sbjct: 1   MGNAIRLL------------RKCLNSHGVSASSGGVSALSRDLLNFETTSQVPEKLGSYV 60

Query: 61  VSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSS+KAQ NWYRK+ EAW+  KP PKTPEEASRLVI  LK HQK DVEGLL+FYGLP PH
Sbjct: 61  VSSQKAQANWYRKILEAWKQAKPRPKTPEEASRLVIAALKNHQKADVEGLLSFYGLPSPH 120

Query: 121 TLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
            LVE+  P   P SLP GV+FEL TLPVD K+VADGDTVTVYVS+  P  SS +P +V  
Sbjct: 121 NLVEV--PTEAPVSLPKGVRFELNTLPVDTKSVADGDTVTVYVSSKDPLVSSSLPKDVSL 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AA++R++AR  KNY +ADALHKTI  +GYR+++ QNEE+LA+K+RIRL GID+PES MPY
Sbjct: 181 AAVKRAKAREKKNYTEADALHKTIIASGYRMISFQNEEVLAKKFRIRLSGIDSPESKMPY 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEA +EL K+VEGKCL+VLVY EDRY RCVGD+YCNGKF+QE MLKKG AWHY AYDKR
Sbjct: 241 GKEAHDELLKMVEGKCLKVLVYTEDRYGRCVGDIYCNGKFVQEVMLKKGLAWHYVAYDKR 300

Query: 301 AELAKWENEARARRIGLWASRNPEQPWEWRKGKREG 337
           AELAKWENEAR +R+GLWAS NPE+PWEWRK KR G
Sbjct: 301 AELAKWENEARQKRVGLWASSNPEKPWEWRKNKRGG 322

[Close]

BLAST of CmaCh04G001200 vs. ExPASy Swiss-Prot
Match: Q942N7 (Probable staphylococcal-like nuclease CAN1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0166100 PE=2 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 1.8e-115
Identity = 209/338 (61.83%), Postives = 251/338 (74.26%), Query Frame = 0


Query: 1   MGNAL-RFLCSHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKH 60
           MGN++ RFLC   C+P     +    PHG  PA   V+AL  D+  FE TSQVP GLS+H
Sbjct: 1   MGNSIYRFLCG-LCSP-----SPEYQPHGAHPA---VAALGRDIQQFEATSQVPDGLSRH 60

Query: 61  VVSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLP 120
           VVSSKKAQ NWY+KL   W+  +P P+TPEEA+RLV+ TLK HQK DVEG L FYGLP+P
Sbjct: 61  VVSSKKAQANWYKKLIVTWKKARPTPRTPEEAARLVVTTLKNHQKADVEGFLVFYGLPIP 120

Query: 121 HTLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVR 180
           +      AP       P G KFEL TLPVDAKAVADGDT+TVY+ TA PRES  +P E++
Sbjct: 121 NAAASTPAPHTAHVPKPQGCKFELHTLPVDAKAVADGDTITVYIDTADPRESGNVPREIQ 180

Query: 181 NAAIQRSQARNAKNYAKADALHKTITDAGYR-VLTVQNEEILARKYRIRLRGIDAPESAM 240
            AA +R++AR A++Y KAD L K I DAGYR V   + EE+LA+KYRIRLRGIDAPESAM
Sbjct: 181 KAAAERTRARAARDYQKADGLQKMIADAGYRQVPNARGEEVLAKKYRIRLRGIDAPESAM 240

Query: 241 PYGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYD 300
           PYGKEAKE L K+V+GK L+V VY EDRY RCVGD+YC+G F+QE MLKKG AWHYTAYD
Sbjct: 241 PYGKEAKEALLKMVQGKSLKVYVYDEDRYGRCVGDIYCDGVFVQEQMLKKGCAWHYTAYD 300

Query: 301 KRAELAKWENEARARRIGLWASRNPEQPWEWRKGKREG 337
           +R ELAKWE +A++ R GLWA+  P++PWEWR+ KR G
Sbjct: 301 QRPELAKWEKQAQSGRKGLWAASRPQKPWEWRRDKRNG 329

[Close]

BLAST of CmaCh04G001200 vs. ExPASy Swiss-Prot
Match: Q2RBJ2 (Probable staphylococcal-like nuclease CAN2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g0109500 PE=2 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 2.6e-98
Identity = 173/323 (53.56%), Postives = 231/323 (71.52%), Query Frame = 0


Query: 15  PNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHVVSSKKAQTNWYRKL 74
           P A      LGPHGV+P+TVGV+ALAHDL +FE TS VP GLS+HVVSS+KAQ  WY+KL
Sbjct: 41  PPAPLQQQHLGPHGVTPSTVGVAALAHDLLNFESTSMVPDGLSQHVVSSRKAQVKWYQKL 100

Query: 75  AEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPHTLVELSAPAPLPTS 134
            EA+++  PPPKTP  A++L+ + L   Q+ D+EG+L FY LP+P   +  ++    P+ 
Sbjct: 101 LEAYKNTTPPPKTPANAAQLIARALNMIQRADLEGILEFYNLPIPS--LPTASSNYQPSL 160

Query: 135 LPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRNAAIQRSQARNAKNY 194
           LP+GV+F L TLPV  K + DGD  T YV T  PRES+ +P+EV    I R+QAR  ++Y
Sbjct: 161 LPEGVQFVLNTLPVYDKCIGDGDGFTAYVPTTDPRESANVPLEVHELVIARTQARKCRDY 220

Query: 195 AKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPYGKEAKEELRKLVEG 254
             ADAL  ++ +AGY++++  ++E+LARKYRIR+RGIDAPE  MPYGKE++  L KL+ G
Sbjct: 221 QSADALLSSLDEAGYKIISCSDDEVLARKYRIRMRGIDAPELKMPYGKESRTALVKLIGG 280

Query: 255 KCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRAELAKWENEARARR 314
           K +++ VY  D++ R VGD+YCN  FIQE MLK G AWH+  YDKR E A+WE EARA  
Sbjct: 281 KSVKIYVYDLDQFGRYVGDIYCNNLFIQEQMLKNGHAWHFKTYDKRPEFARWEREARAAN 340

Query: 315 IGLWASRNPEQPWEWRKGKREGK 338
            GLWAS NPE+PW+WR+ +R  +
Sbjct: 341 RGLWASGNPEKPWDWRRDQRNAR 361

[Close]

BLAST of CmaCh04G001200 vs. ExPASy TrEMBL
Match: A0A6J1K113 (staphylococcal-like nuclease CAN2 OS=Cucurbita maxima OX=3661 GN=LOC111491421 PE=4 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 1.9e-192
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0


Query: 1   MGNALRFLCSHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGNALRFLCSHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV
Sbjct: 1   MGNALRFLCSHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60

Query: 61  VSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH
Sbjct: 61  VSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120

Query: 121 TLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
           TLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN
Sbjct: 121 TLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY
Sbjct: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR
Sbjct: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300

Query: 301 AELAKWENEARARRIGLWASRNPEQPWEWRKGKREGK 338
           AELAKWENEARARRIGLWASRNPEQPWEWRKGKREGK
Sbjct: 301 AELAKWENEARARRIGLWASRNPEQPWEWRKGKREGK 337

[Close]

BLAST of CmaCh04G001200 vs. ExPASy TrEMBL
Match: A0A6J1FPN5 (staphylococcal-like nuclease CAN2 OS=Cucurbita moschata OX=3662 GN=LOC111447091 PE=4 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 3.1e-190
Identity = 332/337 (98.52%), Postives = 334/337 (99.11%), Query Frame = 0


Query: 1   MGNALRFLCSHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGN LRFLC HFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV
Sbjct: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60

Query: 61  VSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSSKKAQ NWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH
Sbjct: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120

Query: 121 TLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
           TLVELSAPAP+PTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN
Sbjct: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY
Sbjct: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR
Sbjct: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300

Query: 301 AELAKWENEARARRIGLWASRNPEQPWEWRKGKREGK 338
           AELAKWENEARA+RIGLWASRNPEQPWEWRKGKREGK
Sbjct: 301 AELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 337

[Close]

BLAST of CmaCh04G001200 vs. ExPASy TrEMBL
Match: A0A6J1CZ71 (staphylococcal-like nuclease CAN2 OS=Momordica charantia OX=3673 GN=LOC111015935 PE=4 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.9e-168
Identity = 301/343 (87.76%), Postives = 314/343 (91.55%), Query Frame = 0


Query: 1   MGNALRFLCSHFCNPNAAA-----DADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQG 60
           MGNALRFLC HFC+P+A +      +DSLGPHGVSPATVGVSALAHDLFDFEITSQVPQG
Sbjct: 1   MGNALRFLCGHFCSPSAGSGGGGGGSDSLGPHGVSPATVGVSALAHDLFDFEITSQVPQG 60

Query: 61  LSKHVVSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYG 120
           LSKHVVSSKKAQ NWY KLAEAWRSEKPPP+TPEEASRLVIQTLKRHQKKDVEGLLAFYG
Sbjct: 61  LSKHVVSSKKAQANWYGKLAEAWRSEKPPPRTPEEASRLVIQTLKRHQKKDVEGLLAFYG 120

Query: 121 LPLPHTLVELSAPAPLP-TSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCI 180
           LPLPHTLVELSA AP     LPDG+KFELQTLPVDAKAVADGDTVTVYVST +PRES+CI
Sbjct: 121 LPLPHTLVELSASAPTTGAPLPDGIKFELQTLPVDAKAVADGDTVTVYVSTENPRESACI 180

Query: 181 PMEVRNAAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAP 240
           P EV+ AA+QRS AR AKNYAKADALHK ITDAGYRVLT QNEEILA+KYRIRLRGIDAP
Sbjct: 181 PKEVQAAAVQRSHARAAKNYAKADALHKKITDAGYRVLTFQNEEILAKKYRIRLRGIDAP 240

Query: 241 ESAMPYGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHY 300
           ES+MPYGKEAKEEL KLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHY
Sbjct: 241 ESSMPYGKEAKEELTKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHY 300

Query: 301 TAYDKRAELAKWENEARARRIGLWASRNPEQPWEWRKGKREGK 338
            AYDKRAELAKWE EARA+R GLWAS NPEQPWEWRK KREGK
Sbjct: 301 AAYDKRAELAKWEKEARAKRAGLWASSNPEQPWEWRKAKREGK 343

[Close]

BLAST of CmaCh04G001200 vs. ExPASy TrEMBL
Match: A0A5D3DQ96 (Staphylococcal-like nuclease CAN1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold436G00570 PE=4 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 1.2e-165
Identity = 289/338 (85.50%), Postives = 313/338 (92.60%), Query Frame = 0


Query: 1   MGNALRFLCSHFCNPNAAADADSLGPH-GVSPATVGVSALAHDLFDFEITSQVPQGLSKH 60
           MGNALRFLC HFC+P     +DSL PH  +S +T GVSALAHDLF+FEITSQVP GL KH
Sbjct: 1   MGNALRFLCGHFCHPT----SDSLPPHAALSSSTAGVSALAHDLFEFEITSQVPPGLGKH 60

Query: 61  VVSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLP 120
           VVSSKKAQ NWY KLAEAW+SEKP P+TPEEASRLVIQTLKRHQKKDVEGLL FYGLPLP
Sbjct: 61  VVSSKKAQANWYGKLAEAWKSEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLP 120

Query: 121 HTLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVR 180
           HTLV+ SAPAP   S+PDGVKFELQTLPVDAKAVADGDTVTV+VST+HPRESSC+P EVR
Sbjct: 121 HTLVKSSAPAPTAASIPDGVKFELQTLPVDAKAVADGDTVTVHVSTSHPRESSCVPKEVR 180

Query: 181 NAAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMP 240
           NAA+QRS+ARNAKNYAKADALHKTITDAGYRV+T QNEE+LA+KYRIRLRGIDAPESAMP
Sbjct: 181 NAALQRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMP 240

Query: 241 YGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300
           YGKEAKEEL++LVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK
Sbjct: 241 YGKEAKEELKRLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300

Query: 301 RAELAKWENEARARRIGLWASRNPEQPWEWRKGKREGK 338
           R EL+KWENEARA+R+GLWAS NP++PWEWRKGKREGK
Sbjct: 301 RPELSKWENEARAKRVGLWASSNPDKPWEWRKGKREGK 334

[Close]

BLAST of CmaCh04G001200 vs. ExPASy TrEMBL
Match: A0A1S3C4Y1 (staphylococcal-like nuclease CAN1 OS=Cucumis melo OX=3656 GN=LOC103496714 PE=4 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 1.2e-165
Identity = 289/338 (85.50%), Postives = 313/338 (92.60%), Query Frame = 0


Query: 1   MGNALRFLCSHFCNPNAAADADSLGPH-GVSPATVGVSALAHDLFDFEITSQVPQGLSKH 60
           MGNALRFLC HFC+P     +DSL PH  +S +T GVSALAHDLF+FEITSQVP GL KH
Sbjct: 1   MGNALRFLCGHFCHPT----SDSLPPHAALSSSTAGVSALAHDLFEFEITSQVPPGLGKH 60

Query: 61  VVSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLP 120
           VVSSKKAQ NWY KLAEAW+SEKP P+TPEEASRLVIQTLKRHQKKDVEGLL FYGLPLP
Sbjct: 61  VVSSKKAQANWYGKLAEAWKSEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLP 120

Query: 121 HTLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVR 180
           HTLV+ SAPAP   S+PDGVKFELQTLPVDAKAVADGDTVTV+VST+HPRESSC+P EVR
Sbjct: 121 HTLVKSSAPAPTAASIPDGVKFELQTLPVDAKAVADGDTVTVHVSTSHPRESSCVPKEVR 180

Query: 181 NAAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMP 240
           NAA+QRS+ARNAKNYAKADALHKTITDAGYRV+T QNEE+LA+KYRIRLRGIDAPESAMP
Sbjct: 181 NAALQRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMP 240

Query: 241 YGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300
           YGKEAKEEL++LVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK
Sbjct: 241 YGKEAKEELKRLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300

Query: 301 RAELAKWENEARARRIGLWASRNPEQPWEWRKGKREGK 338
           R EL+KWENEARA+R+GLWAS NP++PWEWRKGKREGK
Sbjct: 301 RPELSKWENEARAKRVGLWASSNPDKPWEWRKGKREGK 334

[Close]

BLAST of CmaCh04G001200 vs. NCBI nr
Match: XP_022996106.1 (staphylococcal-like nuclease CAN2 [Cucurbita maxima] >XP_022996112.1 staphylococcal-like nuclease CAN2 [Cucurbita maxima])

HSP 1 Score: 681.4 bits (1757), Expect = 3.9e-192
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0


Query: 1   MGNALRFLCSHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGNALRFLCSHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV
Sbjct: 1   MGNALRFLCSHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60

Query: 61  VSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH
Sbjct: 61  VSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120

Query: 121 TLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
           TLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN
Sbjct: 121 TLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY
Sbjct: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR
Sbjct: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300

Query: 301 AELAKWENEARARRIGLWASRNPEQPWEWRKGKREGK 338
           AELAKWENEARARRIGLWASRNPEQPWEWRKGKREGK
Sbjct: 301 AELAKWENEARARRIGLWASRNPEQPWEWRKGKREGK 337

[Close]

BLAST of CmaCh04G001200 vs. NCBI nr
Match: XP_022941859.1 (staphylococcal-like nuclease CAN2 [Cucurbita moschata] >XP_023552645.1 staphylococcal-like nuclease CAN2 [Cucurbita pepo subsp. pepo] >KAG6599960.1 Staphylococcal-like nuclease CAN2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030638.1 Staphylococcal-like nuclease CAN2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 674.1 bits (1738), Expect = 6.3e-190
Identity = 332/337 (98.52%), Postives = 334/337 (99.11%), Query Frame = 0


Query: 1   MGNALRFLCSHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGN LRFLC HFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV
Sbjct: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60

Query: 61  VSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSSKKAQ NWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH
Sbjct: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120

Query: 121 TLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
           TLVELSAPAP+PTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN
Sbjct: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY
Sbjct: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR
Sbjct: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300

Query: 301 AELAKWENEARARRIGLWASRNPEQPWEWRKGKREGK 338
           AELAKWENEARA+RIGLWASRNPEQPWEWRKGKREGK
Sbjct: 301 AELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 337

[Close]

BLAST of CmaCh04G001200 vs. NCBI nr
Match: XP_038892991.1 (staphylococcal-like nuclease CAN1 isoform X1 [Benincasa hispida])

HSP 1 Score: 610.1 bits (1572), Expect = 1.1e-170
Identity = 302/337 (89.61%), Postives = 313/337 (92.88%), Query Frame = 0


Query: 1   MGNALRFLCSHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGN L FLC HFCNP+    +DS  PHGVS AT GVSALAHDLFDFEITSQVPQGLSKHV
Sbjct: 1   MGNVLGFLCGHFCNPS----SDSQPPHGVSYATAGVSALAHDLFDFEITSQVPQGLSKHV 60

Query: 61  VSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSSKKAQ NWYRKLA+AW+ EKPPP+TPEEASRLVIQTLKRHQKKDVEGLL FYGLPLPH
Sbjct: 61  VSSKKAQANWYRKLADAWKLEKPPPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPH 120

Query: 121 TLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
           TLVELSAPAP   SLPDGVKFEL+TLPVDAKAVADGDTVTVYVSTAH RESSCIP+EVRN
Sbjct: 121 TLVELSAPAPTTASLPDGVKFELETLPVDAKAVADGDTVTVYVSTAHTRESSCIPIEVRN 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AAIQRSQARNAKN+AKADALHKTITDAGYRVLT QNEEILARKYRIRLRGIDAPES MPY
Sbjct: 181 AAIQRSQARNAKNFAKADALHKTITDAGYRVLTFQNEEILARKYRIRLRGIDAPESGMPY 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEAK+EL KLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHY AYDKR
Sbjct: 241 GKEAKQELTKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYKAYDKR 300

Query: 301 AELAKWENEARARRIGLWASRNPEQPWEWRKGKREGK 338
           AEL+KWENEARA+R GLWAS NPEQPWEWRKGKREGK
Sbjct: 301 AELSKWENEARAKRAGLWASSNPEQPWEWRKGKREGK 333

[Close]

BLAST of CmaCh04G001200 vs. NCBI nr
Match: XP_022146824.1 (staphylococcal-like nuclease CAN2 [Momordica charantia])

HSP 1 Score: 601.7 bits (1550), Expect = 4.0e-168
Identity = 301/343 (87.76%), Postives = 314/343 (91.55%), Query Frame = 0


Query: 1   MGNALRFLCSHFCNPNAAA-----DADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQG 60
           MGNALRFLC HFC+P+A +      +DSLGPHGVSPATVGVSALAHDLFDFEITSQVPQG
Sbjct: 1   MGNALRFLCGHFCSPSAGSGGGGGGSDSLGPHGVSPATVGVSALAHDLFDFEITSQVPQG 60

Query: 61  LSKHVVSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYG 120
           LSKHVVSSKKAQ NWY KLAEAWRSEKPPP+TPEEASRLVIQTLKRHQKKDVEGLLAFYG
Sbjct: 61  LSKHVVSSKKAQANWYGKLAEAWRSEKPPPRTPEEASRLVIQTLKRHQKKDVEGLLAFYG 120

Query: 121 LPLPHTLVELSAPAPLP-TSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCI 180
           LPLPHTLVELSA AP     LPDG+KFELQTLPVDAKAVADGDTVTVYVST +PRES+CI
Sbjct: 121 LPLPHTLVELSASAPTTGAPLPDGIKFELQTLPVDAKAVADGDTVTVYVSTENPRESACI 180

Query: 181 PMEVRNAAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAP 240
           P EV+ AA+QRS AR AKNYAKADALHK ITDAGYRVLT QNEEILA+KYRIRLRGIDAP
Sbjct: 181 PKEVQAAAVQRSHARAAKNYAKADALHKKITDAGYRVLTFQNEEILAKKYRIRLRGIDAP 240

Query: 241 ESAMPYGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHY 300
           ES+MPYGKEAKEEL KLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHY
Sbjct: 241 ESSMPYGKEAKEELTKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHY 300

Query: 301 TAYDKRAELAKWENEARARRIGLWASRNPEQPWEWRKGKREGK 338
            AYDKRAELAKWE EARA+R GLWAS NPEQPWEWRK KREGK
Sbjct: 301 AAYDKRAELAKWEKEARAKRAGLWASSNPEQPWEWRKAKREGK 343

[Close]

BLAST of CmaCh04G001200 vs. NCBI nr
Match: XP_008456902.1 (PREDICTED: staphylococcal-like nuclease CAN1 [Cucumis melo] >TYK25847.1 staphylococcal-like nuclease CAN1 [Cucumis melo var. makuwa])

HSP 1 Score: 592.4 bits (1526), Expect = 2.4e-165
Identity = 289/338 (85.50%), Postives = 313/338 (92.60%), Query Frame = 0


Query: 1   MGNALRFLCSHFCNPNAAADADSLGPH-GVSPATVGVSALAHDLFDFEITSQVPQGLSKH 60
           MGNALRFLC HFC+P     +DSL PH  +S +T GVSALAHDLF+FEITSQVP GL KH
Sbjct: 1   MGNALRFLCGHFCHPT----SDSLPPHAALSSSTAGVSALAHDLFEFEITSQVPPGLGKH 60

Query: 61  VVSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLP 120
           VVSSKKAQ NWY KLAEAW+SEKP P+TPEEASRLVIQTLKRHQKKDVEGLL FYGLPLP
Sbjct: 61  VVSSKKAQANWYGKLAEAWKSEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLP 120

Query: 121 HTLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVR 180
           HTLV+ SAPAP   S+PDGVKFELQTLPVDAKAVADGDTVTV+VST+HPRESSC+P EVR
Sbjct: 121 HTLVKSSAPAPTAASIPDGVKFELQTLPVDAKAVADGDTVTVHVSTSHPRESSCVPKEVR 180

Query: 181 NAAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMP 240
           NAA+QRS+ARNAKNYAKADALHKTITDAGYRV+T QNEE+LA+KYRIRLRGIDAPESAMP
Sbjct: 181 NAALQRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMP 240

Query: 241 YGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300
           YGKEAKEEL++LVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK
Sbjct: 241 YGKEAKEELKRLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300

Query: 301 RAELAKWENEARARRIGLWASRNPEQPWEWRKGKREGK 338
           R EL+KWENEARA+R+GLWAS NP++PWEWRKGKREGK
Sbjct: 301 RPELSKWENEARAKRVGLWASSNPDKPWEWRKGKREGK 334

[Close]

BLAST of CmaCh04G001200 vs. TAIR 10
Match: AT2G40410.2 (Staphylococcal nuclease homologue )

HSP 1 Score: 472.2 bits (1214), Expect = 3.4e-133
Identity = 234/334 (70.06%), Postives = 271/334 (81.14%), Query Frame = 0


Query: 1   MGNALRFLCSHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGNAL FL    C P      DSLGPHGVS ATVGVSALAHDLF+FEITSQVP+GL ++V
Sbjct: 1   MGNALTFLYGKCCKPTTT--DDSLGPHGVSAATVGVSALAHDLFNFEITSQVPEGLGRYV 60

Query: 61  VSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
            SS+KAQ NWYRK+ EAW+  KPPP+T EEASRLV   LKR+QK DVEGLL+FYGLPL H
Sbjct: 61  QSSRKAQANWYRKILEAWKQAKPPPQTAEEASRLVTDILKRNQKADVEGLLSFYGLPLSH 120

Query: 121 TLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
           TLVE++  AP+  SLP+G+ FE QTLPVD KAVADGDT+TVYVST+ P  SS +P EV  
Sbjct: 121 TLVEVTVEAPV--SLPEGILFEFQTLPVDPKAVADGDTITVYVSTSEPVVSSSVPREVNL 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AA+QR++AR  +NY KAD LH+ I D+GYRVL ++NEE+LARK+RIRLRGIDAPES MP+
Sbjct: 181 AAVQRAKAREKRNYPKADELHQKIIDSGYRVLNIENEEVLARKFRIRLRGIDAPESQMPF 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEA+E L K+V  K L+VLVYGED+Y RCVGDLYCNG F+QEAMLKKG AWHY AYDKR
Sbjct: 241 GKEAQEGLLKIVGRKSLKVLVYGEDQYGRCVGDLYCNGIFVQEAMLKKGLAWHYLAYDKR 300

Query: 301 AELAKWENEARARRIGLWASRNPEQPWEWRKGKR 335
             LAKWE EAR +RIGLWAS NPE+PW+WRK  R
Sbjct: 301 PVLAKWEKEARQKRIGLWASSNPEKPWDWRKNNR 330

[Close]

BLAST of CmaCh04G001200 vs. TAIR 10
Match: AT3G56170.1 (Ca-2+ dependent nuclease )

HSP 1 Score: 453.4 bits (1165), Expect = 1.6e-127
Identity = 225/336 (66.96%), Postives = 263/336 (78.27%), Query Frame = 0


Query: 1   MGNALRFLCSHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGNA+R L               L  HGVS ++ GVSAL+ DL +FE TSQVP+ L  +V
Sbjct: 1   MGNAIRLL------------RKCLNSHGVSASSGGVSALSRDLLNFETTSQVPEKLGSYV 60

Query: 61  VSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSS+KAQ NWYRK+ EAW+  KP PKTPEEASRLVI  LK HQK DVEGLL+FYGLP PH
Sbjct: 61  VSSQKAQANWYRKILEAWKQAKPRPKTPEEASRLVIAALKNHQKADVEGLLSFYGLPSPH 120

Query: 121 TLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
            LVE+  P   P SLP GV+FEL TLPVD K+VADGDTVTVYVS+  P  SS +P +V  
Sbjct: 121 NLVEV--PTEAPVSLPKGVRFELNTLPVDTKSVADGDTVTVYVSSKDPLVSSSLPKDVSL 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AA++R++AR  KNY +ADALHKTI  +GYR+++ QNEE+LA+K+RIRL GID+PES MPY
Sbjct: 181 AAVKRAKAREKKNYTEADALHKTIIASGYRMISFQNEEVLAKKFRIRLSGIDSPESKMPY 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEA +EL K+VEGKCL+VLVY EDRY RCVGD+YCNGKF+QE MLKKG AWHY AYDKR
Sbjct: 241 GKEAHDELLKMVEGKCLKVLVYTEDRYGRCVGDIYCNGKFVQEVMLKKGLAWHYVAYDKR 300

Query: 301 AELAKWENEARARRIGLWASRNPEQPWEWRKGKREG 337
           AELAKWENEAR +R+GLWAS NPE+PWEWRK KR G
Sbjct: 301 AELAKWENEARQKRVGLWASSNPEKPWEWRKNKRGG 322

[Close]

BLAST of CmaCh04G001200 vs. TAIR 10
Match: AT2G40410.1 (Staphylococcal nuclease homologue )

HSP 1 Score: 303.1 bits (775), Expect = 2.7e-82
Identity = 155/229 (67.69%), Postives = 183/229 (79.91%), Query Frame = 0


Query: 1   MGNALRFLCSHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGNAL FL    C P      DSLGPHGVS ATVGVSALAHDLF+FEITSQVP+GL ++V
Sbjct: 1   MGNALTFLYGKCCKPTTT--DDSLGPHGVSAATVGVSALAHDLFNFEITSQVPEGLGRYV 60

Query: 61  VSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
            SS+KAQ NWYRK+ EAW+  KPPP+T EEASRLV   LKR+QK DVEGLL+FYGLPL H
Sbjct: 61  QSSRKAQANWYRKILEAWKQAKPPPQTAEEASRLVTDILKRNQKADVEGLLSFYGLPLSH 120

Query: 121 TLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
           TLVE++  AP+  SLP+G+ FE QTLPVD KAVADGDT+TVYVST+ P  SS +P EV  
Sbjct: 121 TLVEVTVEAPV--SLPEGILFEFQTLPVDPKAVADGDTITVYVSTSEPVVSSSVPREVNL 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLR 230
           AA+QR++AR  +NY KAD LH+ I D+GYRVL ++NEE+LARK+RIRLR
Sbjct: 181 AAVQRAKAREKRNYPKADELHQKIIDSGYRVLNIENEEVLARKFRIRLR 225

The following BLAST results are available for this feature:

BLAST of CmaCh04G001200 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4IH31	4.8e-132	70.06	Staphylococcal-like nuclease CAN2 OS=Arabidopsis thaliana OX=3702 GN=CAN2 PE=1 S...	[more]
Q39635	1.8e-126	67.46	Uncharacterized 38.1 kDa protein OS=Capnoides sempervirens OX=3464 PE=2 SV=1	[more]
F4IZC5	2.3e-126	66.96	Staphylococcal-like nuclease CAN1 OS=Arabidopsis thaliana OX=3702 GN=CAN1 PE=1 S...	[more]
Q942N7	1.8e-115	61.83	Probable staphylococcal-like nuclease CAN1 OS=Oryza sativa subsp. japonica OX=39...	[more]
Q2RBJ2	2.6e-98	53.56	Probable staphylococcal-like nuclease CAN2 OS=Oryza sativa subsp. japonica OX=39...	[more]

BLAST of CmaCh04G001200 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K113	1.9e-192	100.00	staphylococcal-like nuclease CAN2 OS=Cucurbita maxima OX=3661 GN=LOC111491421 PE...	[more]
A0A6J1FPN5	3.1e-190	98.52	staphylococcal-like nuclease CAN2 OS=Cucurbita moschata OX=3662 GN=LOC111447091 ...	[more]
A0A6J1CZ71	1.9e-168	87.76	staphylococcal-like nuclease CAN2 OS=Momordica charantia OX=3673 GN=LOC111015935...	[more]
A0A5D3DQ96	1.2e-165	85.50	Staphylococcal-like nuclease CAN1 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3C4Y1	1.2e-165	85.50	staphylococcal-like nuclease CAN1 OS=Cucumis melo OX=3656 GN=LOC103496714 PE=4 S...	[more]

BLAST of CmaCh04G001200 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022996106.1	3.9e-192	100.00	staphylococcal-like nuclease CAN2 [Cucurbita maxima] >XP_022996112.1 staphylococ...	[more]
XP_022941859.1	6.3e-190	98.52	staphylococcal-like nuclease CAN2 [Cucurbita moschata] >XP_023552645.1 staphyloc...	[more]
XP_038892991.1	1.1e-170	89.61	staphylococcal-like nuclease CAN1 isoform X1 [Benincasa hispida]	[more]
XP_022146824.1	4.0e-168	87.76	staphylococcal-like nuclease CAN2 [Momordica charantia]	[more]
XP_008456902.1	2.4e-165	85.50	PREDICTED: staphylococcal-like nuclease CAN1 [Cucumis melo] >TYK25847.1 staphylo...	[more]

BLAST of CmaCh04G001200 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G40410.2	3.4e-133	70.06	Staphylococcal nuclease homologue	[more]
AT3G56170.1	1.6e-127	66.96	Ca-2+ dependent nuclease	[more]
AT2G40410.1	2.7e-82	67.69	Staphylococcal nuclease homologue	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016071	Staphylococcal nuclease (SNase-like), OB-fold	SMART	SM00318	SNASE_2	coord: 202..320 e-value: 1.2E-32 score: 124.4
IPR016071	Staphylococcal nuclease (SNase-like), OB-fold	PFAM	PF00565	SNase	coord: 225..319 e-value: 1.2E-27 score: 96.5
IPR016071	Staphylococcal nuclease (SNase-like), OB-fold	PROSITE	PS50830	TNASE_3	coord: 144..320 score: 31.586191
IPR035437	SNase-like, OB-fold superfamily	GENE3D	2.40.50.90		coord: 141..324 e-value: 7.7E-31 score: 109.0
None	No IPR available	PANTHER	PTHR12302:SF21	STAPHYLOCOCCAL-LIKE NUCLEASE CAN2	coord: 15..332
None	No IPR available	PANTHER	PTHR12302	EBNA2 BINDING PROTEIN P100	coord: 15..332
None	No IPR available	SUPERFAMILY	50199	Staphylococcal nuclease	coord: 146..327

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G001200.1	CmaCh04G001200.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0006418	tRNA aminoacylation for protein translation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004518	nuclease activity
molecular_function	GO:0003676	nucleic acid binding