CmoCh07G012040.1 (mRNA) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh07G012040.1
TypemRNA
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionspermidine hydroxycinnamoyl transferase-like
LocationCmo_Chr07: 6555172 .. 6557537 (-)
Sequence length1656
RNA-Seq ExpressionCmoCh07G012040.1
SyntenyCmoCh07G012040.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAAGTGGATAACTTTACTTATATAAAGGGTAGCTTACAAAACATCTCTCCAATCCAAGCTATTGAAAACAACGTGAATGGCGTCTATAACCATACATTCCTCCTCCACCGTCGTCCCCAGCCACCCTACTCCCACCGGCGCGTTCTCCTTCTCCGAGTGCGACCAATACAGGGCATGGACTCACCTCCCCAGCGTCTACGTCTACAAATCCCCGGCAGCCGAGGTCGTCGCCGTCGAGTCCTTAAAAACCTCCCTCAGCCAAATCCTTGTCCCTTACTACCCTCTCGCCGGGCGTCTCCACTGGCTCCCTGGCGGCCGCCTCGAGCTCGATTGCTGCGCTGCTGGAGCCCTATTCGTCGAAGCTTCTACTGACGCCAAGCTGGAAGATTACGGGGACTTTGCGCCGTCGGATGCTGTAAGAGAACTCGTCCCGTCCGTGGATTACAATTCTCCGATCGAAGAGCTGCCGTTGCTTCTATTTCAAGTGACGAGATTCAGGTGCGGTGGGGTTGTAATTGGCGTAGCTAATTGTCATACGGTGGTGGATGGCGCTTCTGCCATCACGTTCATTAACTCGTGGGCGAGCATGGCGCGTGGAGAGAAGACGGTGGAGACGATTTTGCAGCCGTTGCATGATCGGAAATTTCTGATGCCGGAGAAGCCGCTGCGTCCGCCGCGGTTCGACCACGTTGAATTTACTAAACCACCCCCAGTTATCCTTGGCACCTCCAACTCTGAGGTAAGGATTTTTAAAAGCTTAAAATTTAATATTATAGTATAATAGATTATGCTTAAAAAATACATATTAATGAGTAAGTATATAAAGAAAGAAATATATATAGTTTCGTATTTTAATATATATATATATATATATATATATATATATATATATATATATTATAGATAGATAGATATAAAAGCCGAATGTGAATTTGGGTGAGGTCAAGAACGGAGAGTATGATTATCGTCTCAGGCTCTGTTTTGTTAATGGAGAGAGATTTCTTTTCGCTCCTTACCCAAACGAAGTTTTCTCACCTCGTTCAGGTGGGTCCTAGTGAAGCCTCGATTCTATGGGTTAAACGGACATATGAGATCTCACATTGGTATTTCTTTATAAGGGTTTGGAAACCTCTCTTTAATATATACGTTTTAAAACTGTTAGCCTGATAACTATACATAGTGGGTCAAATCAGACAATATTTGCTAGCAGTGAACTTAAGTTGTTACAAGACATGTGCACTGTGGTCTGTTTCCGTTATTGTAGGATGTTAATTTCTATTTAGTCTTCAAATTTAAAAAATAATTAGTTTTTCAATTTATTTGTGAAATCAAGTAAAATTTATATACCGTCCAATGAATGTTCTATTCGACTAAACTCGAACCATTTATTATTATTGAGTAAACCCCACGAAATTGACTAAAGTTTGTTATTACTATTCTCAATCGAAGGAGGCAAAGCAAGAAACCACATCCGCATTGCTCAAACTTACTAAAGAACAAATTGAAAAGCTGAAAAAGAGAGCAAATTCAAACCTAACCAATAAAACTATGGGCGTGGGCGATGAAGTACAACCACGACCATACTCTCGATTTGAGGCTATTACCGGACACATGTGGGTTTGTGCATGCAAGGCTCGCAATACCGGCGAGAGTCGACAGCCGACGATAGTTCGTACCCCTATTGACGTCAGAAGACGAATGAAGCCACCCATCCCAGAAAACTTCTCAGGGAACTCATCTTTCGTCGTCTTAACACCAGAATGCGAGTTCGGCGAATTGATGTCTCAACCATTGAGCTATGCTGCAGGAAAGGTAAGAGAAGGAACATGGAAGATGACAGGCGAATACGCGCAATCAGTGGTAGACTATCTAGCAGGGCAAGACGACATTAACTGGTTGAGAACATCATTCCACTCCAAGGTTCCAGTCCAGGCTTCGTTCTGGGGCAACCCTAATCTTTCAATAGGGAGTTGGATGTCGTTGCCGTTTTATGAGGCAGATTTTGGCTGGGGACGACCGAGTTACTTCGGGCCAGCAACATTGAACATCGATGGGAAGTCGTACATAATGGTGGGGCCATCAGATGATGGGTCGCTCATAGTAGCGATACGGTTGCAAACAAGACATATGGATGATTTCAAGAAGTATTTCTACGAAGATATATAAAAGTTCGAGTAACACTGAAATCGTTAGTGTTGAGTAATCTCTATATTTCCGTTTTTTCTTGCATGGTCTTCATGGTTGTCCCCATAGTTTGTTCTAAAGCCAAACTATGTTCTTTTTTCTATGTAATATTTCGTTCAGTTTTGGTTTGTCATGTTGATGATATTTAATCTTTTATTTCGGTTTACCATATGCATTATAT

mRNA sequence

TAAAGTGGATAACTTTACTTATATAAAGGGTAGCTTACAAAACATCTCTCCAATCCAAGCTATTGAAAACAACGTGAATGGCGTCTATAACCATACATTCCTCCTCCACCGTCGTCCCCAGCCACCCTACTCCCACCGGCGCGTTCTCCTTCTCCGAGTGCGACCAATACAGGGCATGGACTCACCTCCCCAGCGTCTACGTCTACAAATCCCCGGCAGCCGAGGTCGTCGCCGTCGAGTCCTTAAAAACCTCCCTCAGCCAAATCCTTGTCCCTTACTACCCTCTCGCCGGGCGTCTCCACTGGCTCCCTGGCGGCCGCCTCGAGCTCGATTGCTGCGCTGCTGGAGCCCTATTCGTCGAAGCTTCTACTGACGCCAAGCTGGAAGATTACGGGGACTTTGCGCCGTCGGATGCTGTAAGAGAACTCGTCCCGTCCGTGGATTACAATTCTCCGATCGAAGAGCTGCCGTTGCTTCTATTTCAAGTGACGAGATTCAGGTGCGGTGGGGTTGTAATTGGCGTAGCTAATTGTCATACGGTGGTGGATGGCGCTTCTGCCATCACGTTCATTAACTCGTGGGCGAGCATGGCGCGTGGAGAGAAGACGGTGGAGACGATTTTGCAGCCGTTGCATGATCGGAAATTTCTGATGCCGGAGAAGCCGCTGCGTCCGCCGCGGTTCGACCACGTTGAATTTACTAAACCACCCCCAGTTATCCTTGGCACCTCCAACTCTGAGGAGGCAAAGCAAGAAACCACATCCGCATTGCTCAAACTTACTAAAGAACAAATTGAAAAGCTGAAAAAGAGAGCAAATTCAAACCTAACCAATAAAACTATGGGCGTGGGCGATGAAGTACAACCACGACCATACTCTCGATTTGAGGCTATTACCGGACACATGTGGGTTTGTGCATGCAAGGCTCGCAATACCGGCGAGAGTCGACAGCCGACGATAGTTCGTACCCCTATTGACGTCAGAAGACGAATGAAGCCACCCATCCCAGAAAACTTCTCAGGGAACTCATCTTTCGTCGTCTTAACACCAGAATGCGAGTTCGGCGAATTGATGTCTCAACCATTGAGCTATGCTGCAGGAAAGGTAAGAGAAGGAACATGGAAGATGACAGGCGAATACGCGCAATCAGTGGTAGACTATCTAGCAGGGCAAGACGACATTAACTGGTTGAGAACATCATTCCACTCCAAGGTTCCAGTCCAGGCTTCGTTCTGGGGCAACCCTAATCTTTCAATAGGGAGTTGGATGTCGTTGCCGTTTTATGAGGCAGATTTTGGCTGGGGACGACCGAGTTACTTCGGGCCAGCAACATTGAACATCGATGGGAAGTCGTACATAATGGTGGGGCCATCAGATGATGGGTCGCTCATAGTAGCGATACGGTTGCAAACAAGACATATGGATGATTTCAAGAAGTATTTCTACGAAGATATATAAAAGTTCGAGTAACACTGAAATCGTTAGTGTTGAGTAATCTCTATATTTCCGTTTTTTCTTGCATGGTCTTCATGGTTGTCCCCATAGTTTGTTCTAAAGCCAAACTATGTTCTTTTTTCTATGTAATATTTCGTTCAGTTTTGGTTTGTCATGTTGATGATATTTAATCTTTTATTTCGGTTTACCATATGCATTATAT

Coding sequence (CDS)

ATGGCGTCTATAACCATACATTCCTCCTCCACCGTCGTCCCCAGCCACCCTACTCCCACCGGCGCGTTCTCCTTCTCCGAGTGCGACCAATACAGGGCATGGACTCACCTCCCCAGCGTCTACGTCTACAAATCCCCGGCAGCCGAGGTCGTCGCCGTCGAGTCCTTAAAAACCTCCCTCAGCCAAATCCTTGTCCCTTACTACCCTCTCGCCGGGCGTCTCCACTGGCTCCCTGGCGGCCGCCTCGAGCTCGATTGCTGCGCTGCTGGAGCCCTATTCGTCGAAGCTTCTACTGACGCCAAGCTGGAAGATTACGGGGACTTTGCGCCGTCGGATGCTGTAAGAGAACTCGTCCCGTCCGTGGATTACAATTCTCCGATCGAAGAGCTGCCGTTGCTTCTATTTCAAGTGACGAGATTCAGGTGCGGTGGGGTTGTAATTGGCGTAGCTAATTGTCATACGGTGGTGGATGGCGCTTCTGCCATCACGTTCATTAACTCGTGGGCGAGCATGGCGCGTGGAGAGAAGACGGTGGAGACGATTTTGCAGCCGTTGCATGATCGGAAATTTCTGATGCCGGAGAAGCCGCTGCGTCCGCCGCGGTTCGACCACGTTGAATTTACTAAACCACCCCCAGTTATCCTTGGCACCTCCAACTCTGAGGAGGCAAAGCAAGAAACCACATCCGCATTGCTCAAACTTACTAAAGAACAAATTGAAAAGCTGAAAAAGAGAGCAAATTCAAACCTAACCAATAAAACTATGGGCGTGGGCGATGAAGTACAACCACGACCATACTCTCGATTTGAGGCTATTACCGGACACATGTGGGTTTGTGCATGCAAGGCTCGCAATACCGGCGAGAGTCGACAGCCGACGATAGTTCGTACCCCTATTGACGTCAGAAGACGAATGAAGCCACCCATCCCAGAAAACTTCTCAGGGAACTCATCTTTCGTCGTCTTAACACCAGAATGCGAGTTCGGCGAATTGATGTCTCAACCATTGAGCTATGCTGCAGGAAAGGTAAGAGAAGGAACATGGAAGATGACAGGCGAATACGCGCAATCAGTGGTAGACTATCTAGCAGGGCAAGACGACATTAACTGGTTGAGAACATCATTCCACTCCAAGGTTCCAGTCCAGGCTTCGTTCTGGGGCAACCCTAATCTTTCAATAGGGAGTTGGATGTCGTTGCCGTTTTATGAGGCAGATTTTGGCTGGGGACGACCGAGTTACTTCGGGCCAGCAACATTGAACATCGATGGGAAGTCGTACATAATGGTGGGGCCATCAGATGATGGGTCGCTCATAGTAGCGATACGGTTGCAAACAAGACATATGGATGATTTCAAGAAGTATTTCTACGAAGATATATAA

Protein sequence

MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKSPAAEVVAVESLKTSLSQILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPSVDYNSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVETILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSNSEEAKQETTSALLKLTKEQIEKLKKRANSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPIDVRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVVDYLAGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATLNIDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI
Homology
BLAST of CmoCh07G012040.1 vs. ExPASy Swiss-Prot
Match: O64470 (Spermidine hydroxycinnamoyl transferase OS=Arabidopsis thaliana OX=3702 GN=SHT PE=1 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 6.8e-105
Identity = 201/465 (43.23%), Postives = 283/465 (60.86%), Query Frame = 0

Query: 1   MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKSPAAEVV--AVESLKT 60
           MA IT   S T+VP+ PT +G F  +E DQ    TH+P++Y Y  P+       VE LKT
Sbjct: 1   MAPITFRKSYTIVPAEPTWSGRFPLAEWDQVGTITHIPTLYFYDKPSESFQGNVVEILKT 60

Query: 61  SLSQILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELV 120
           SLS++LV +YP+AGRL WLP GR EL+C A G  F+EA ++ KL D+ DF+P+     L+
Sbjct: 61  SLSRVLVHFYPMAGRLRWLPRGRFELNCNAEGVEFIEAESEGKLSDFKDFSPTPEFENLM 120

Query: 121 PSVDYNSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTV 180
           P V+Y +PIE +PL L QVT+F+CGG+ + V   H +VDG SA+  I+ W  +ARGE  +
Sbjct: 121 PQVNYKNPIETIPLFLAQVTKFKCGGISLSVNVSHAIVDGQSALHLISEWGRLARGE-PL 180

Query: 181 ETILQPLHDRKFLMPEKPL----RPPRFDHVEFTKPPPVILGTSNSEEAKQETTSALLKL 240
           ET+  P  DRK L   +PL     PP+FDH EF +PP +I  T N EE K++T   +L L
Sbjct: 181 ETV--PFLDRKILWAGEPLPPFVSPPKFDHKEFDQPPFLIGETDNVEERKKKTIVVMLPL 240

Query: 241 TKEQIEKLKKRANSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTI 300
           +  Q++KL+ +AN        G       + ++R+E +TGH+W CACKAR      QPT 
Sbjct: 241 STSQLQKLRSKAN--------GSKHSDPAKGFTRYETVTGHVWRCACKARG-HSPEQPTA 300

Query: 301 VRTPIDVRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEY 360
           +   ID R RM+PP+P  + GN++  V+      GEL+S  L +AA  + +    +T EY
Sbjct: 301 LGICIDTRSRMEPPLPRGYFGNATLDVVAASTS-GELISNELGFAASLISKAIKNVTNEY 360

Query: 361 AQSVVDYLAGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYF 420
               ++YL  Q D+   +   H+    +  F+GNPNL + SW++LP Y  DFGWG+  Y 
Sbjct: 361 VMIGIEYLKNQKDLKKFQ-DLHALGSTEGPFYGNPNLGVVSWLTLPMYGLDFGWGKEFYT 420

Query: 421 GPATLNIDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI 460
           GP T + DG S I+   ++DGS+I+A  LQ  HM+ FKK+FYEDI
Sbjct: 421 GPGTHDFDGDSLILPDQNEDGSVILATCLQVAHMEAFKKHFYEDI 451

BLAST of CmoCh07G012040.1 vs. ExPASy Swiss-Prot
Match: Q8GSM7 (Shikimate O-hydroxycinnamoyltransferase OS=Nicotiana tabacum OX=4097 GN=HST PE=1 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 3.1e-81
Identity = 175/457 (38.29%), Postives = 257/457 (56.24%), Query Frame = 0

Query: 4   ITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYK-SPAAEVVAVESLKTSLSQ 63
           I +  S+ V P+  TP      S  D      H PSVY Y+ + +      + LK +LS+
Sbjct: 3   IEVKESTMVKPAAETPQQRLWNSNVDLVVPNFHTPSVYFYRPTGSPNFFDGKVLKEALSK 62

Query: 64  ILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPSVD 123
            LVP+YP+AGRL     GR+E+DC   G LFVEA +D  ++D+GDFAP+  +R+L+P+VD
Sbjct: 63  ALVPFYPMAGRLCRDEDGRIEIDCKGQGVLFVEAESDGVVDDFGDFAPTLELRQLIPAVD 122

Query: 124 YNSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVETIL 183
           Y+  I+   LL+ Q+T F+CGGV +GV   H   DGAS + FIN+W+ MARG   ++  +
Sbjct: 123 YSQGIQSYALLVLQITHFKCGGVSLGVGMQHHAADGASGLHFINTWSDMARG---LDLTI 182

Query: 184 QPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSNS--EEAKQETTSALLKLTKEQIE 243
            P  DR  L    P + P+F HVE+  PP + +   N+   EA  ET+ ++ KLT++QI 
Sbjct: 183 PPFIDRTLLRARDPPQ-PQFPHVEYQPPPTLKVTPENTPISEAVPETSVSIFKLTRDQIN 242

Query: 244 KLKKRANSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPID 303
            LK ++  +        G+ V    YS +E + GH+W   C AR      Q T +    D
Sbjct: 243 TLKAKSKED--------GNTVN---YSSYEMLAGHVWRSTCMARGLAHD-QETKLYIATD 302

Query: 304 VRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVVD 363
            R R++P +P  + GN  F   TP    G++ S+P+ YAA K+ +   +M  +Y +S +D
Sbjct: 303 GRSRLRPSLPPGYFGNVIFTT-TPIAVAGDIQSKPIWYAASKLHDALARMDNDYLRSALD 362

Query: 364 YLAGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATLN 423
           YL  Q D+  L    H+        +  PNL I SW  LP ++ADFGWGRP + GP  + 
Sbjct: 363 YLELQPDLKALVRGAHT--------FKCPNLGITSWSRLPIHDADFGWGRPIFMGPGGIA 422

Query: 424 IDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYE 458
            +G S+I+  P++DGS  VAI LQ  HM  F+K+ Y+
Sbjct: 423 YEGLSFILPSPTNDGSQSVAISLQAEHMKLFEKFLYD 434

BLAST of CmoCh07G012040.1 vs. ExPASy Swiss-Prot
Match: Q9FI78 (Shikimate O-hydroxycinnamoyltransferase OS=Arabidopsis thaliana OX=3702 GN=HST PE=1 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 4.4e-80
Identity = 173/455 (38.02%), Postives = 257/455 (56.48%), Query Frame = 0

Query: 4   ITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYK-SPAAEVVAVESLKTSLSQ 63
           I I  S+ V P+  TP      S  D      H PSVY Y+ + A+     + +K +LS+
Sbjct: 3   INIRDSTMVRPATETPITNLWNSNVDLVIPRFHTPSVYFYRPTGASNFFDPQVMKEALSK 62

Query: 64  ILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPSVD 123
            LVP+YP+AGRL     GR+E+DC  AG LFV A T + ++D+GDFAP+  +R+L+P VD
Sbjct: 63  ALVPFYPMAGRLKRDDDGRIEIDCNGAGVLFVVADTPSVIDDFGDFAPTLNLRQLIPEVD 122

Query: 124 YNSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVETIL 183
           +++ I   PLL+ QVT F+CGG  +GV   H   DG S + FIN+W+ MARG   ++  +
Sbjct: 123 HSAGIHSFPLLVLQVTFFKCGGASLGVGMQHHAADGFSGLHFINTWSDMARG---LDLTI 182

Query: 184 QPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSNSEEAKQETTSALLKLTKEQIEKL 243
            P  DR  L    P + P F HVE+   P + +    S+   + TT ++ KLT++Q+  L
Sbjct: 183 PPFIDRTLLRARDPPQ-PAFHHVEYQPAPSMKIPLDPSKSGPENTTVSIFKLTRDQLVAL 242

Query: 244 KKRANSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPIDVR 303
           K ++  +        G+ V    YS +E + GH+W    KAR    + Q T +    D R
Sbjct: 243 KAKSKED--------GNTVS---YSSYEMLAGHVWRSVGKARGL-PNDQETKLYIATDGR 302

Query: 304 RRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVVDYL 363
            R++P +P  + GN  F   TP    G+L+S+P  YAAG++ +   +M   Y +S +DYL
Sbjct: 303 SRLRPQLPPGYFGNVIFTA-TPLAVAGDLLSKPTWYAAGQIHDFLVRMDDNYLRSALDYL 362

Query: 364 AGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATLNID 423
             Q D++ L    H+        +  PNL I SW+ LP Y+ADFGWGRP + GP  +  +
Sbjct: 363 EMQPDLSALVRGAHT--------YKCPNLGITSWVRLPIYDADFGWGRPIFMGPGGIPYE 422

Query: 424 GKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYE 458
           G S+++  P++DGSL VAI LQ+ HM  F+K+ +E
Sbjct: 423 GLSFVLPSPTNDGSLSVAIALQSEHMKLFEKFLFE 432

BLAST of CmoCh07G012040.1 vs. ExPASy Swiss-Prot
Match: A0A2H5AIZ1 (Hydroxycinnamoyltransferase OS=Narcissus pseudonarcissus OX=39639 GN=HCT PE=2 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 3.9e-76
Identity = 168/465 (36.13%), Postives = 247/465 (53.12%), Query Frame = 0

Query: 4   ITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKSP--AAEVVAVESLKTSLS 63
           I I  ++ V PS PTP+     S  D      H PSVY Y+ P  A++      LK +L 
Sbjct: 3   INIKETTMVRPSQPTPSQRLWNSNLDLVVPRFHTPSVYFYRRPGNASDFFDARVLKEALG 62

Query: 64  QILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPSV 123
           + LVP+YP+AGRL     GR+E+DC   G  FV A TD+ ++++GDFAP+  +++L+P V
Sbjct: 63  RALVPFYPMAGRLARDEDGRVEIDCNGEGVRFVVAETDSAIDEFGDFAPTMELKKLIPKV 122

Query: 124 DYNSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVETI 183
           +Y   I   PLL+ Q+T F+CGG  +GV   H V DGAS + FINSW+ +ARG   ++  
Sbjct: 123 EYGDDISAFPLLVLQITHFKCGGTSLGVGMQHHVADGASGLHFINSWSDIARG---LDIA 182

Query: 184 LQPLHDRKFLMPEKPLRPPRFDHVEF-------TKPPPVILGTSNSEEAKQETTSALLKL 243
           + P  DR  L    P   P F H+E+       T P P+   T  S+     T  ++ KL
Sbjct: 183 VPPFIDRSLLRARDP-PSPSFPHIEYQPAPSMNTSPAPIQDPTVKSDPT--ATAVSIFKL 242

Query: 244 TKEQIEKLKKRANSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTI 303
           TK+Q++ LK R ++                 YS +  + GH+W C   AR   +  Q T 
Sbjct: 243 TKQQLDLLKSRVSAK----------------YSSYALVAGHVWRCTSIARGLPDD-QRTK 302

Query: 304 VRTPIDVRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQP--LSYAAGKVREGTWKMTG 363
           +    D R R++PP+P  + GN  F   TP  + GE+  +   L  AAG+++    +M  
Sbjct: 303 LYCATDGRARLQPPLPSGYFGNVIFTA-TPVADAGEITGEDSGLEAAAGRIQRALMRMDD 362

Query: 364 EYAQSVVDYLAGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPS 423
           EY +S +DYL  Q D++ L    H+        +  PN+ + SW  LP ++ADFGWGRP 
Sbjct: 363 EYLRSALDYLELQPDLSKLVRGAHT--------FRCPNIGLTSWTRLPIHDADFGWGRPI 422

Query: 424 YFGPATLNIDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYE 458
           + GP  +  +G ++++     DGSL +AI LQ  HM  F+K  YE
Sbjct: 423 FMGPGGIAYEGLAFMLPSSEGDGSLSIAISLQAEHMIKFQKLLYE 435

BLAST of CmoCh07G012040.1 vs. ExPASy Swiss-Prot
Match: A0PDV5 (Rosmarinate synthase OS=Plectranthus scutellarioides OX=4142 GN=RAS PE=1 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 2.1e-74
Identity = 175/456 (38.38%), Postives = 245/456 (53.73%), Query Frame = 0

Query: 4   ITIHSSSTVVPSHPTPTGAFSFSECDQYR-AWTHLPSVYVYKSPAAE-VVAVESLKTSLS 63
           I +  S+ + PS  TP G+   S  D    A  H  SV+ Y    ++       LK SLS
Sbjct: 3   IEVKDSTMIKPSAETPGGSLWLSNLDLLSPANYHTLSVHFYSHDGSDNFFDAAGLKESLS 62

Query: 64  QILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPSV 123
           + LV +YP AGRL  L G RLE+DC   G L VEA  D  L++ GDFAP   +  L+P V
Sbjct: 63  RALVEFYPYAGRLK-LNGNRLEIDCNNEGLLLVEAECDGALDELGDFAPRPEL-NLIPKV 122

Query: 124 DYNSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVETI 183
           DY+  I   PL++FQ+TRF+CGGV +GVAN H + DG +A+ FIN+WA ++RG      +
Sbjct: 123 DYSRGISTYPLMVFQLTRFKCGGVALGVANEHHLSDGVAALHFINTWAHLSRGAPAPTPL 182

Query: 184 LQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSNSEEAKQETTSALLKLTKEQIEK 243
             P  DR  L    P + P+F H E+  PP     T  +     +   +  KLT++Q+  
Sbjct: 183 --PHFDRSSLSARNPPQ-PQFSHAEYQPPP-----TLENPLPHTDIAHSRFKLTRDQLNS 242

Query: 244 LKKRANSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPIDV 303
           LK +  +   +   G       + YS FE + GH+W   C AR   E  Q T +  P D 
Sbjct: 243 LKSKFKTAPADGGAG-------KSYSTFEVLAGHIWRSVCIARGLPEG-QETKLHIPFDG 302

Query: 304 RRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVVDY 363
           R R++  +P  F GN+ F   TP    GE+ S  L+YA  +V +G  ++  +Y +S +D+
Sbjct: 303 RGRLQ--LPPGFFGNAIFFA-TPIATCGEIESNSLNYAVRRVSDGVSRLDEDYLRSSIDF 362

Query: 364 LAGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATLNI 423
           L  Q+DI+ L    HS        +  PNL + SW+ LP YE DFGWG+  Y GP     
Sbjct: 363 LELQEDISKLAQGAHS--------FRCPNLWVISWVWLPIYEPDFGWGKAVYMGPWAAPF 422

Query: 424 DGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYE 458
           +GKSY++  P  DGSL V+I L  +HM+ F+K FYE
Sbjct: 423 EGKSYLLPNPEKDGSLFVSITLHKQHMERFEKLFYE 429

BLAST of CmoCh07G012040.1 vs. ExPASy TrEMBL
Match: A0A6J1EEM2 (spermidine hydroxycinnamoyl transferase-like OS=Cucurbita moschata OX=3662 GN=LOC111432536 PE=3 SV=1)

HSP 1 Score: 941.4 bits (2432), Expect = 1.4e-270
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 0

Query: 1   MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKSPAAEVVAVESLKTSL 60
           MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKSPAAEVVAVESLKTSL
Sbjct: 1   MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKSPAAEVVAVESLKTSL 60

Query: 61  SQILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPS 120
           SQILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPS
Sbjct: 61  SQILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPS 120

Query: 121 VDYNSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVET 180
           VDYNSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVET
Sbjct: 121 VDYNSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVET 180

Query: 181 ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSNSEEAKQETTSALLKLTKEQIE 240
           ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSNSEEAKQETTSALLKLTKEQIE
Sbjct: 181 ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSNSEEAKQETTSALLKLTKEQIE 240

Query: 241 KLKKRANSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPID 300
           KLKKRANSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPID
Sbjct: 241 KLKKRANSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPID 300

Query: 301 VRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVVD 360
           VRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVVD
Sbjct: 301 VRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVVD 360

Query: 361 YLAGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATLN 420
           YLAGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATLN
Sbjct: 361 YLAGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATLN 420

Query: 421 IDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI 460
           IDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI
Sbjct: 421 IDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI 459

BLAST of CmoCh07G012040.1 vs. ExPASy TrEMBL
Match: A0A6J1EBK1 (spermidine hydroxycinnamoyl transferase-like OS=Cucurbita moschata OX=3662 GN=LOC111432537 PE=3 SV=1)

HSP 1 Score: 918.3 bits (2372), Expect = 1.3e-263
Identity = 445/459 (96.95%), Postives = 451/459 (98.26%), Query Frame = 0

Query: 1   MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKSPAAEVVAVESLKTSL 60
           MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPS+YVYKSPAAEVVAVESLKTSL
Sbjct: 1   MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSIYVYKSPAAEVVAVESLKTSL 60

Query: 61  SQILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPS 120
           SQILVPYYPLAGRLHWLPGGR ELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPS
Sbjct: 61  SQILVPYYPLAGRLHWLPGGRFELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPS 120

Query: 121 VDYNSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVET 180
           VDYNS IEELPLLLFQVTRF CGGVVIGVA CHTVVDGASA+TFINSWASMARGEKTVET
Sbjct: 121 VDYNSSIEELPLLLFQVTRFSCGGVVIGVATCHTVVDGASAVTFINSWASMARGEKTVET 180

Query: 181 ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSNSEEAKQETTSALLKLTKEQIE 240
           ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPP+ILGTSN EEA QETTSALLKLTKEQIE
Sbjct: 181 ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPLILGTSNPEEANQETTSALLKLTKEQIE 240

Query: 241 KLKKRANSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPID 300
           KLKKRANSN+TNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPID
Sbjct: 241 KLKKRANSNVTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPID 300

Query: 301 VRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVVD 360
           VRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEY QSVVD
Sbjct: 301 VRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYVQSVVD 360

Query: 361 YLAGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATLN 420
           YLAGQD+INWLRTSFHSKVPVQASFWGNPNLSIGSWMS PFYEA+FGWGRPSYFGPATLN
Sbjct: 361 YLAGQDNINWLRTSFHSKVPVQASFWGNPNLSIGSWMSFPFYEANFGWGRPSYFGPATLN 420

Query: 421 IDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI 460
           IDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI
Sbjct: 421 IDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI 459

BLAST of CmoCh07G012040.1 vs. ExPASy TrEMBL
Match: A0A6J1HNZ2 (spermidine hydroxycinnamoyl transferase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466005 PE=3 SV=1)

HSP 1 Score: 903.3 bits (2333), Expect = 4.2e-259
Identity = 434/459 (94.55%), Postives = 449/459 (97.82%), Query Frame = 0

Query: 1   MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKSPAAEVVAVESLKTSL 60
           MASITIHSSSTVVPSHPTPTG FSFSECDQYRAWTHLP++YVYKSPA EVVAVESLKTSL
Sbjct: 1   MASITIHSSSTVVPSHPTPTGTFSFSECDQYRAWTHLPTIYVYKSPATEVVAVESLKTSL 60

Query: 61  SQILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPS 120
           SQILVPYYPLAGRLHWLPGGRLELDCCAAG LFVEASTDAKLEDYGDFAPSDAVR+L+P+
Sbjct: 61  SQILVPYYPLAGRLHWLPGGRLELDCCAAGVLFVEASTDAKLEDYGDFAPSDAVRQLIPT 120

Query: 121 VDYNSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVET 180
           VDYNSPIEELPLLLFQVTRF CGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVET
Sbjct: 121 VDYNSPIEELPLLLFQVTRFSCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVET 180

Query: 181 ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSNSEEAKQETTSALLKLTKEQIE 240
           ILQPLHDRKFLMPEKPLRPPRFDH+EFTKPPPVILG+SN EEAKQETTSALLKLTKEQIE
Sbjct: 181 ILQPLHDRKFLMPEKPLRPPRFDHIEFTKPPPVILGSSNPEEAKQETTSALLKLTKEQIE 240

Query: 241 KLKKRANSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPID 300
           KLKK+ANSNLTN+TMGV DEVQPRPYSRFEAITGHMWVCACKARNTGESRQPT++R PID
Sbjct: 241 KLKKKANSNLTNQTMGVADEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTVIRIPID 300

Query: 301 VRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVVD 360
           VRRRM+PPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVVD
Sbjct: 301 VRRRMQPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVVD 360

Query: 361 YLAGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATLN 420
           YLAGQDDI+WLRTSFHSKV V+ASFWGNPNLSIGSW+SLPFYEADFGWGRPSYFGPATLN
Sbjct: 361 YLAGQDDISWLRTSFHSKVLVKASFWGNPNLSIGSWISLPFYEADFGWGRPSYFGPATLN 420

Query: 421 IDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI 460
           IDGKSYIMVGPSDDGSLIVAIRLQ RHMD FKKYFYEDI
Sbjct: 421 IDGKSYIMVGPSDDGSLIVAIRLQARHMDGFKKYFYEDI 459

BLAST of CmoCh07G012040.1 vs. ExPASy TrEMBL
Match: A0A6J1HTD7 (spermidine hydroxycinnamoyl transferase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466005 PE=3 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 7.9e-258
Identity = 434/460 (94.35%), Postives = 449/460 (97.61%), Query Frame = 0

Query: 1   MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKSPAAEVVAVESLKTSL 60
           MASITIHSSSTVVPSHPTPTG FSFSECDQYRAWTHLP++YVYKSPA EVVAVESLKTSL
Sbjct: 1   MASITIHSSSTVVPSHPTPTGTFSFSECDQYRAWTHLPTIYVYKSPATEVVAVESLKTSL 60

Query: 61  SQILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPS 120
           SQILVPYYPLAGRLHWLPGGRLELDCCAAG LFVEASTDAKLEDYGDFAPSDAVR+L+P+
Sbjct: 61  SQILVPYYPLAGRLHWLPGGRLELDCCAAGVLFVEASTDAKLEDYGDFAPSDAVRQLIPT 120

Query: 121 VDYNSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVET 180
           VDYNSPIEELPLLLFQVTRF CGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVET
Sbjct: 121 VDYNSPIEELPLLLFQVTRFSCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVET 180

Query: 181 ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSN-SEEAKQETTSALLKLTKEQI 240
           ILQPLHDRKFLMPEKPLRPPRFDH+EFTKPPPVILG+SN  EEAKQETTSALLKLTKEQI
Sbjct: 181 ILQPLHDRKFLMPEKPLRPPRFDHIEFTKPPPVILGSSNPEEEAKQETTSALLKLTKEQI 240

Query: 241 EKLKKRANSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPI 300
           EKLKK+ANSNLTN+TMGV DEVQPRPYSRFEAITGHMWVCACKARNTGESRQPT++R PI
Sbjct: 241 EKLKKKANSNLTNQTMGVADEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTVIRIPI 300

Query: 301 DVRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVV 360
           DVRRRM+PPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVV
Sbjct: 301 DVRRRMQPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVV 360

Query: 361 DYLAGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATL 420
           DYLAGQDDI+WLRTSFHSKV V+ASFWGNPNLSIGSW+SLPFYEADFGWGRPSYFGPATL
Sbjct: 361 DYLAGQDDISWLRTSFHSKVLVKASFWGNPNLSIGSWISLPFYEADFGWGRPSYFGPATL 420

Query: 421 NIDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI 460
           NIDGKSYIMVGPSDDGSLIVAIRLQ RHMD FKKYFYEDI
Sbjct: 421 NIDGKSYIMVGPSDDGSLIVAIRLQARHMDGFKKYFYEDI 460

BLAST of CmoCh07G012040.1 vs. ExPASy TrEMBL
Match: A0A6J1EB80 (spermidine hydroxycinnamoyl transferase-like OS=Cucurbita moschata OX=3662 GN=LOC111432535 PE=3 SV=1)

HSP 1 Score: 889.8 bits (2298), Expect = 4.8e-255
Identity = 431/460 (93.70%), Postives = 445/460 (96.74%), Query Frame = 0

Query: 1   MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKSPAAEVVAVESLKTSL 60
           MASITIHSSSTV+PSHPTPTGAFS SECDQYRAWTHLPSVYVYKSPAAEV+ VESLKTSL
Sbjct: 1   MASITIHSSSTVIPSHPTPTGAFSVSECDQYRAWTHLPSVYVYKSPAAEVIIVESLKTSL 60

Query: 61  SQILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPS 120
           SQILVP+YPLAGRLHWLPGGRLELDCCAAGALFVEAST+AKLEDYGDFAPSDAVRELVPS
Sbjct: 61  SQILVPFYPLAGRLHWLPGGRLELDCCAAGALFVEASTNAKLEDYGDFAPSDAVRELVPS 120

Query: 121 VDYNSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVET 180
           VDYNSPIEELPLLLFQVTRF CGGVVIGVANCHTVVDGASA+TFINSWASMARGEKTVET
Sbjct: 121 VDYNSPIEELPLLLFQVTRFSCGGVVIGVANCHTVVDGASAVTFINSWASMARGEKTVET 180

Query: 181 ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSNS-EEAKQETTSALLKLTKEQI 240
           ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPP+ILG+SN+ EEAK+ETTSALLKLTKEQI
Sbjct: 181 ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPLILGSSNTEEEAKKETTSALLKLTKEQI 240

Query: 241 EKLKKRANSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPI 300
           EKLKKRANSNLTN+TM V DEVQPRPYSRFEAITGHMWVCACKARNTGESRQPT++R PI
Sbjct: 241 EKLKKRANSNLTNQTMDVADEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTVIRIPI 300

Query: 301 DVRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVV 360
           DVRRRMKPPIPENFSGNSSFVV TPECEFGELMSQPLSYAAGK+REGTWKMTGEYAQS V
Sbjct: 301 DVRRRMKPPIPENFSGNSSFVVSTPECEFGELMSQPLSYAAGKIREGTWKMTGEYAQSAV 360

Query: 361 DYLAGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATL 420
           DYLAGQDD N LRTSFHSKVPVQASFWGNPNLSIGSWMS PFYEADFGWGRPSYF PATL
Sbjct: 361 DYLAGQDDFNRLRTSFHSKVPVQASFWGNPNLSIGSWMSFPFYEADFGWGRPSYFTPATL 420

Query: 421 NIDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI 460
           N DGKSYIMVGPSDDGSLI+AIRLQTRHMDDFKKY YEDI
Sbjct: 421 NTDGKSYIMVGPSDDGSLIIAIRLQTRHMDDFKKYLYEDI 460

BLAST of CmoCh07G012040.1 vs. NCBI nr
Match: XP_022925233.1 (spermidine hydroxycinnamoyl transferase-like [Cucurbita moschata] >KAG6595494.1 Spermidine hydroxycinnamoyl transferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 941.4 bits (2432), Expect = 2.9e-270
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 0

Query: 1   MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKSPAAEVVAVESLKTSL 60
           MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKSPAAEVVAVESLKTSL
Sbjct: 1   MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKSPAAEVVAVESLKTSL 60

Query: 61  SQILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPS 120
           SQILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPS
Sbjct: 61  SQILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPS 120

Query: 121 VDYNSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVET 180
           VDYNSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVET
Sbjct: 121 VDYNSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVET 180

Query: 181 ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSNSEEAKQETTSALLKLTKEQIE 240
           ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSNSEEAKQETTSALLKLTKEQIE
Sbjct: 181 ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSNSEEAKQETTSALLKLTKEQIE 240

Query: 241 KLKKRANSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPID 300
           KLKKRANSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPID
Sbjct: 241 KLKKRANSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPID 300

Query: 301 VRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVVD 360
           VRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVVD
Sbjct: 301 VRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVVD 360

Query: 361 YLAGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATLN 420
           YLAGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATLN
Sbjct: 361 YLAGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATLN 420

Query: 421 IDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI 460
           IDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI
Sbjct: 421 IDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI 459

BLAST of CmoCh07G012040.1 vs. NCBI nr
Match: XP_023517444.1 (spermidine hydroxycinnamoyl transferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 931.4 bits (2406), Expect = 3.0e-267
Identity = 453/459 (98.69%), Postives = 456/459 (99.35%), Query Frame = 0

Query: 1   MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKSPAAEVVAVESLKTSL 60
           MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKSPAAEVVAVESLKTSL
Sbjct: 1   MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKSPAAEVVAVESLKTSL 60

Query: 61  SQILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPS 120
           SQILVPYYPLAGRLHWLPGGRLELDCCAAG LFVEASTDAKLEDYGDFAPSDAVRELVPS
Sbjct: 61  SQILVPYYPLAGRLHWLPGGRLELDCCAAGTLFVEASTDAKLEDYGDFAPSDAVRELVPS 120

Query: 121 VDYNSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVET 180
           VDYNSPIEELPLLLFQVTRF CGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVET
Sbjct: 121 VDYNSPIEELPLLLFQVTRFSCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVET 180

Query: 181 ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSNSEEAKQETTSALLKLTKEQIE 240
           ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSN EEAKQETTSALLKLTKEQIE
Sbjct: 181 ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSNPEEAKQETTSALLKLTKEQIE 240

Query: 241 KLKKRANSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPID 300
           KLKKRANSN+TN+TMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPID
Sbjct: 241 KLKKRANSNVTNQTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPID 300

Query: 301 VRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVVD 360
           VRRRMKPPIPENFSGNSSFVVLTPECEFGEL+SQPLSYAAGKVREGTWKMTGEYAQSVVD
Sbjct: 301 VRRRMKPPIPENFSGNSSFVVLTPECEFGELISQPLSYAAGKVREGTWKMTGEYAQSVVD 360

Query: 361 YLAGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATLN 420
           YLAGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATLN
Sbjct: 361 YLAGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATLN 420

Query: 421 IDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI 460
           IDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI
Sbjct: 421 IDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI 459

BLAST of CmoCh07G012040.1 vs. NCBI nr
Match: KAG6595492.1 (Spermidine hydroxycinnamoyl transferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 922.2 bits (2382), Expect = 1.8e-264
Identity = 448/459 (97.60%), Postives = 452/459 (98.47%), Query Frame = 0

Query: 1   MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKSPAAEVVAVESLKTSL 60
           MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKSPAAEVVAVESLKTSL
Sbjct: 1   MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKSPAAEVVAVESLKTSL 60

Query: 61  SQILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPS 120
           SQILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPS
Sbjct: 61  SQILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPS 120

Query: 121 VDYNSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVET 180
           VDYNS IEELPLLLFQVTRF CGGVVIGVA CHTVVDGASA+TFINSWASMARGEKTVET
Sbjct: 121 VDYNSSIEELPLLLFQVTRFSCGGVVIGVATCHTVVDGASAVTFINSWASMARGEKTVET 180

Query: 181 ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSNSEEAKQETTSALLKLTKEQIE 240
           ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPP+ILGTSN EEA QETTSALLKLTKEQIE
Sbjct: 181 ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPLILGTSNPEEANQETTSALLKLTKEQIE 240

Query: 241 KLKKRANSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPID 300
           KLKKRANSN+TNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPID
Sbjct: 241 KLKKRANSNVTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPID 300

Query: 301 VRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVVD 360
           VRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEY QSVVD
Sbjct: 301 VRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYVQSVVD 360

Query: 361 YLAGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATLN 420
           YLAGQD+INWLRTSFHSKVPVQASFWGNPNLSIGSWMS PFYEADFGWGRPSYFGPATLN
Sbjct: 361 YLAGQDNINWLRTSFHSKVPVQASFWGNPNLSIGSWMSFPFYEADFGWGRPSYFGPATLN 420

Query: 421 IDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI 460
           IDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI
Sbjct: 421 IDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI 459

BLAST of CmoCh07G012040.1 vs. NCBI nr
Match: XP_023517487.1 (spermidine hydroxycinnamoyl transferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 920.2 bits (2377), Expect = 6.9e-264
Identity = 448/459 (97.60%), Postives = 454/459 (98.91%), Query Frame = 0

Query: 1   MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKSPAAEVVAVESLKTSL 60
           MASITIHSSST+VPSHPTPTGAFS SECDQYRAWTHLPSVYVYKSPAAEVVAVESLKTSL
Sbjct: 1   MASITIHSSSTIVPSHPTPTGAFSVSECDQYRAWTHLPSVYVYKSPAAEVVAVESLKTSL 60

Query: 61  SQILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPS 120
           SQILVPYYPLAGRLHWL GGRLELDCCAAGALF EAST+AKLEDYGDFAPSDAVRELVPS
Sbjct: 61  SQILVPYYPLAGRLHWLLGGRLELDCCAAGALFFEASTNAKLEDYGDFAPSDAVRELVPS 120

Query: 121 VDYNSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVET 180
           VDYNSPIEELPLLLFQVTRF CGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVET
Sbjct: 121 VDYNSPIEELPLLLFQVTRFSCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVET 180

Query: 181 ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSNSEEAKQETTSALLKLTKEQIE 240
           ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSN EEAKQETTSALLKLTKEQIE
Sbjct: 181 ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSNPEEAKQETTSALLKLTKEQIE 240

Query: 241 KLKKRANSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPID 300
           KLKKRANSN+TN+TMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPID
Sbjct: 241 KLKKRANSNVTNQTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPID 300

Query: 301 VRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVVD 360
           VRRRMKPPIPENFSGNSSFVVLTPECEFGEL+SQPLSYAAGKVREGTWKMTGEYAQSVVD
Sbjct: 301 VRRRMKPPIPENFSGNSSFVVLTPECEFGELISQPLSYAAGKVREGTWKMTGEYAQSVVD 360

Query: 361 YLAGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATLN 420
           YLAGQD+INWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATLN
Sbjct: 361 YLAGQDNINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATLN 420

Query: 421 IDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI 460
           IDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI
Sbjct: 421 IDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI 459

BLAST of CmoCh07G012040.1 vs. NCBI nr
Match: XP_022925234.1 (spermidine hydroxycinnamoyl transferase-like [Cucurbita moschata])

HSP 1 Score: 918.3 bits (2372), Expect = 2.6e-263
Identity = 445/459 (96.95%), Postives = 451/459 (98.26%), Query Frame = 0

Query: 1   MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKSPAAEVVAVESLKTSL 60
           MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPS+YVYKSPAAEVVAVESLKTSL
Sbjct: 1   MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSIYVYKSPAAEVVAVESLKTSL 60

Query: 61  SQILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPS 120
           SQILVPYYPLAGRLHWLPGGR ELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPS
Sbjct: 61  SQILVPYYPLAGRLHWLPGGRFELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPS 120

Query: 121 VDYNSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVET 180
           VDYNS IEELPLLLFQVTRF CGGVVIGVA CHTVVDGASA+TFINSWASMARGEKTVET
Sbjct: 121 VDYNSSIEELPLLLFQVTRFSCGGVVIGVATCHTVVDGASAVTFINSWASMARGEKTVET 180

Query: 181 ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSNSEEAKQETTSALLKLTKEQIE 240
           ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPP+ILGTSN EEA QETTSALLKLTKEQIE
Sbjct: 181 ILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPLILGTSNPEEANQETTSALLKLTKEQIE 240

Query: 241 KLKKRANSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPID 300
           KLKKRANSN+TNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPID
Sbjct: 241 KLKKRANSNVTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPID 300

Query: 301 VRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVVD 360
           VRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEY QSVVD
Sbjct: 301 VRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYVQSVVD 360

Query: 361 YLAGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATLN 420
           YLAGQD+INWLRTSFHSKVPVQASFWGNPNLSIGSWMS PFYEA+FGWGRPSYFGPATLN
Sbjct: 361 YLAGQDNINWLRTSFHSKVPVQASFWGNPNLSIGSWMSFPFYEANFGWGRPSYFGPATLN 420

Query: 421 IDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI 460
           IDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI
Sbjct: 421 IDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI 459

BLAST of CmoCh07G012040.1 vs. TAIR 10
Match: AT2G19070.1 (spermidine hydroxycinnamoyl transferase )

HSP 1 Score: 382.5 bits (981), Expect = 4.8e-106
Identity = 201/465 (43.23%), Postives = 283/465 (60.86%), Query Frame = 0

Query: 1   MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKSPAAEVV--AVESLKT 60
           MA IT   S T+VP+ PT +G F  +E DQ    TH+P++Y Y  P+       VE LKT
Sbjct: 1   MAPITFRKSYTIVPAEPTWSGRFPLAEWDQVGTITHIPTLYFYDKPSESFQGNVVEILKT 60

Query: 61  SLSQILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELV 120
           SLS++LV +YP+AGRL WLP GR EL+C A G  F+EA ++ KL D+ DF+P+     L+
Sbjct: 61  SLSRVLVHFYPMAGRLRWLPRGRFELNCNAEGVEFIEAESEGKLSDFKDFSPTPEFENLM 120

Query: 121 PSVDYNSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTV 180
           P V+Y +PIE +PL L QVT+F+CGG+ + V   H +VDG SA+  I+ W  +ARGE  +
Sbjct: 121 PQVNYKNPIETIPLFLAQVTKFKCGGISLSVNVSHAIVDGQSALHLISEWGRLARGE-PL 180

Query: 181 ETILQPLHDRKFLMPEKPL----RPPRFDHVEFTKPPPVILGTSNSEEAKQETTSALLKL 240
           ET+  P  DRK L   +PL     PP+FDH EF +PP +I  T N EE K++T   +L L
Sbjct: 181 ETV--PFLDRKILWAGEPLPPFVSPPKFDHKEFDQPPFLIGETDNVEERKKKTIVVMLPL 240

Query: 241 TKEQIEKLKKRANSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTI 300
           +  Q++KL+ +AN        G       + ++R+E +TGH+W CACKAR      QPT 
Sbjct: 241 STSQLQKLRSKAN--------GSKHSDPAKGFTRYETVTGHVWRCACKARG-HSPEQPTA 300

Query: 301 VRTPIDVRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEY 360
           +   ID R RM+PP+P  + GN++  V+      GEL+S  L +AA  + +    +T EY
Sbjct: 301 LGICIDTRSRMEPPLPRGYFGNATLDVVAASTS-GELISNELGFAASLISKAIKNVTNEY 360

Query: 361 AQSVVDYLAGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYF 420
               ++YL  Q D+   +   H+    +  F+GNPNL + SW++LP Y  DFGWG+  Y 
Sbjct: 361 VMIGIEYLKNQKDLKKFQ-DLHALGSTEGPFYGNPNLGVVSWLTLPMYGLDFGWGKEFYT 420

Query: 421 GPATLNIDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI 460
           GP T + DG S I+   ++DGS+I+A  LQ  HM+ FKK+FYEDI
Sbjct: 421 GPGTHDFDGDSLILPDQNEDGSVILATCLQVAHMEAFKKHFYEDI 451

BLAST of CmoCh07G012040.1 vs. TAIR 10
Match: AT5G48930.1 (hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase )

HSP 1 Score: 300.1 bits (767), Expect = 3.1e-81
Identity = 173/455 (38.02%), Postives = 257/455 (56.48%), Query Frame = 0

Query: 4   ITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYK-SPAAEVVAVESLKTSLSQ 63
           I I  S+ V P+  TP      S  D      H PSVY Y+ + A+     + +K +LS+
Sbjct: 3   INIRDSTMVRPATETPITNLWNSNVDLVIPRFHTPSVYFYRPTGASNFFDPQVMKEALSK 62

Query: 64  ILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPSVD 123
            LVP+YP+AGRL     GR+E+DC  AG LFV A T + ++D+GDFAP+  +R+L+P VD
Sbjct: 63  ALVPFYPMAGRLKRDDDGRIEIDCNGAGVLFVVADTPSVIDDFGDFAPTLNLRQLIPEVD 122

Query: 124 YNSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVETIL 183
           +++ I   PLL+ QVT F+CGG  +GV   H   DG S + FIN+W+ MARG   ++  +
Sbjct: 123 HSAGIHSFPLLVLQVTFFKCGGASLGVGMQHHAADGFSGLHFINTWSDMARG---LDLTI 182

Query: 184 QPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSNSEEAKQETTSALLKLTKEQIEKL 243
            P  DR  L    P + P F HVE+   P + +    S+   + TT ++ KLT++Q+  L
Sbjct: 183 PPFIDRTLLRARDPPQ-PAFHHVEYQPAPSMKIPLDPSKSGPENTTVSIFKLTRDQLVAL 242

Query: 244 KKRANSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPIDVR 303
           K ++  +        G+ V    YS +E + GH+W    KAR    + Q T +    D R
Sbjct: 243 KAKSKED--------GNTVS---YSSYEMLAGHVWRSVGKARGL-PNDQETKLYIATDGR 302

Query: 304 RRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVVDYL 363
            R++P +P  + GN  F   TP    G+L+S+P  YAAG++ +   +M   Y +S +DYL
Sbjct: 303 SRLRPQLPPGYFGNVIFTA-TPLAVAGDLLSKPTWYAAGQIHDFLVRMDDNYLRSALDYL 362

Query: 364 AGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATLNID 423
             Q D++ L    H+        +  PNL I SW+ LP Y+ADFGWGRP + GP  +  +
Sbjct: 363 EMQPDLSALVRGAHT--------YKCPNLGITSWVRLPIYDADFGWGRPIFMGPGGIPYE 422

Query: 424 GKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYE 458
           G S+++  P++DGSL VAI LQ+ HM  F+K+ +E
Sbjct: 423 GLSFVLPSPTNDGSLSVAIALQSEHMKLFEKFLFE 432

BLAST of CmoCh07G012040.1 vs. TAIR 10
Match: AT5G57840.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 211.5 bits (537), Expect = 1.5e-54
Identity = 145/460 (31.52%), Postives = 221/460 (48.04%), Query Frame = 0

Query: 4   ITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYK-SPAAEVVAVESLKTSLSQ 63
           I +  ++ V P+  TPT     S  D  +   H+ ++Y YK   +++    +SL  +LS+
Sbjct: 3   IRVKQATIVKPAEETPTHNLWLSNLDLIQVRLHMGTLYFYKPCSSSDRPNTQSLIEALSK 62

Query: 64  ILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELVPSVD 123
           +LV +YP AGRL     GRLE+ C   G LFVEA +D+ ++D G    S  + +LVP+VD
Sbjct: 63  VLVFFYPAAGRLQKNTNGRLEVQCNGEGVLFVEAESDSTVQDIGLLTQSLDLSQLVPTVD 122

Query: 124 YNSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVETIL 183
           Y   I   PLLLFQVT F+CG + +G +  HT  +  S    + +W+  ARG   +   L
Sbjct: 123 YAGDISSYPLLLFQVTYFKCGTICVGSSIHHTFGEATSLGYIMEAWSLTARG---LLVKL 182

Query: 184 QPLHDRKFLMPEKPLRPPRFDHVEFTKPP---PVILGTSNSEEAKQETTSALLKLTKEQI 243
            P  DR  L    P     F H E+  PP     +   +     + ++  A LKLT+ Q+
Sbjct: 183 TPFLDRTVLHARNP-PSSVFPHTEYQSPPFHNHPMKSLAYRSNPESDSAIATLKLTRLQL 242

Query: 244 EKLKKRANSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPI 303
           + LK RA             E+    +S +E +  H W CA  A         T +   I
Sbjct: 243 KALKARA-------------EIADSKFSTYEVLVAHTWRCASFANEDLSEEHSTRLHIII 302

Query: 304 DVRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVV 363
           D R R++P +P+ + GN+ F    P    G    +  S    +V     KM  EY +S +
Sbjct: 303 DGRPRLQPKLPQGYIGNTLFHA-RPVSPLGAFHRESFSETVERVHREIRKMDNEYLRSAI 362

Query: 364 DYLAGQDDINWLRTSFHSKVPVQAS--FWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPA 423
           DYL    D++ L       VP + +  F    N  I S +    YE DFGWG+  Y   +
Sbjct: 363 DYLERHPDLDQL-------VPGEGNTIFSCAANFCIVSLIKQTAYEMDFGWGKAYYKRAS 422

Query: 424 TLNIDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYE 458
            LN +GK ++   P ++GS+++ + L+   +  F+K FYE
Sbjct: 423 HLN-EGKGFVTGNPDEEGSMMLTMCLKKTQLSKFRKLFYE 436

BLAST of CmoCh07G012040.1 vs. TAIR 10
Match: AT5G41040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 193.4 bits (490), Expect = 4.1e-49
Identity = 146/452 (32.30%), Postives = 214/452 (47.35%), Query Frame = 0

Query: 12  VVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKS-PAAEVVAVESLKTSLSQILVPYYPL 71
           V P   T  G +  S  DQ  A   + ++Y +KS       AV+ +K +LSQ+LV YYPL
Sbjct: 41  VKPESETRKGLYFLSNLDQNIA-VIVRTIYCFKSEERGNEEAVQVIKKALSQVLVHYYPL 100

Query: 72  AGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSD--AVRELVPSVDYNSPIE 131
           AGRL   P G+L +DC   G +FVEA  + K+++ GD    D   + +LV  V     I 
Sbjct: 101 AGRLTISPEGKLTVDCTEEGVVFVEAEANCKMDEIGDITKPDPETLGKLVYDVVDAKNIL 160

Query: 132 ELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVETILQPLHDR 191
           E+P +  QVT+F+CGG V+G+   H + DG  A+ F+NSW  +ARG   +     P  DR
Sbjct: 161 EIPPVTAQVTKFKCGGFVLGLCMNHCMFDGIGAMEFVNSWGQVARG---LPLTTPPFSDR 220

Query: 192 KFLMPEKPLRPPRFD--HVEFTKPPPVILGTSNSEEAKQETTSALLKLTKEQIEKLKKRA 251
             L       PP+ +  H EF +         NS   K+ T         E+I+KLK +A
Sbjct: 221 TIL---NARNPPKIENLHQEFEEIED--KSNINSLYTKEPTLYRSFCFDPEKIKKLKLQA 280

Query: 252 NSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPIDVRRRMK 311
             N         + +     + FEA++  +W    K+     S Q T +   +D R + +
Sbjct: 281 TEN--------SESLLGNSCTSFEALSAFVWRARTKSLKM-LSDQKTKLLFAVDGRAKFE 340

Query: 312 PPIPENFSGNSSFVVLTPE-CEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVVDYLAGQ 371
           P +P+ + GN   +VLT   CE GEL+ +PLS+A G VRE    +T  Y +S +DY    
Sbjct: 341 PQLPKGYFGNG--IVLTNSICEAGELIEKPLSFAVGLVREAIKMVTDGYMRSAIDYF--- 400

Query: 372 DDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATLNIDGKS 431
            ++   R S  S             L I +W  L F+  DFGWG P   GP  L     +
Sbjct: 401 -EVTRARPSLSS------------TLLITTWSRLGFHTTDFGWGEPILSGPVALPEKEVT 456

Query: 432 YIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYE 458
             +       S+ V + L    MD F++ F +
Sbjct: 461 LFLSHGEQRRSINVLLGLPATAMDVFQEQFLQ 456

BLAST of CmoCh07G012040.1 vs. TAIR 10
Match: AT5G41040.2 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 193.4 bits (490), Expect = 4.1e-49
Identity = 146/452 (32.30%), Postives = 214/452 (47.35%), Query Frame = 0

Query: 12  VVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKS-PAAEVVAVESLKTSLSQILVPYYPL 71
           V P   T  G +  S  DQ  A   + ++Y +KS       AV+ +K +LSQ+LV YYPL
Sbjct: 25  VKPESETRKGLYFLSNLDQNIA-VIVRTIYCFKSEERGNEEAVQVIKKALSQVLVHYYPL 84

Query: 72  AGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSD--AVRELVPSVDYNSPIE 131
           AGRL   P G+L +DC   G +FVEA  + K+++ GD    D   + +LV  V     I 
Sbjct: 85  AGRLTISPEGKLTVDCTEEGVVFVEAEANCKMDEIGDITKPDPETLGKLVYDVVDAKNIL 144

Query: 132 ELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVETILQPLHDR 191
           E+P +  QVT+F+CGG V+G+   H + DG  A+ F+NSW  +ARG   +     P  DR
Sbjct: 145 EIPPVTAQVTKFKCGGFVLGLCMNHCMFDGIGAMEFVNSWGQVARG---LPLTTPPFSDR 204

Query: 192 KFLMPEKPLRPPRFD--HVEFTKPPPVILGTSNSEEAKQETTSALLKLTKEQIEKLKKRA 251
             L       PP+ +  H EF +         NS   K+ T         E+I+KLK +A
Sbjct: 205 TIL---NARNPPKIENLHQEFEEIED--KSNINSLYTKEPTLYRSFCFDPEKIKKLKLQA 264

Query: 252 NSNLTNKTMGVGDEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTIVRTPIDVRRRMK 311
             N         + +     + FEA++  +W    K+     S Q T +   +D R + +
Sbjct: 265 TEN--------SESLLGNSCTSFEALSAFVWRARTKSLKM-LSDQKTKLLFAVDGRAKFE 324

Query: 312 PPIPENFSGNSSFVVLTPE-CEFGELMSQPLSYAAGKVREGTWKMTGEYAQSVVDYLAGQ 371
           P +P+ + GN   +VLT   CE GEL+ +PLS+A G VRE    +T  Y +S +DY    
Sbjct: 325 PQLPKGYFGNG--IVLTNSICEAGELIEKPLSFAVGLVREAIKMVTDGYMRSAIDYF--- 384

Query: 372 DDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGRPSYFGPATLNIDGKS 431
            ++   R S  S             L I +W  L F+  DFGWG P   GP  L     +
Sbjct: 385 -EVTRARPSLSS------------TLLITTWSRLGFHTTDFGWGEPILSGPVALPEKEVT 440

Query: 432 YIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYE 458
             +       S+ V + L    MD F++ F +
Sbjct: 445 LFLSHGEQRRSINVLLGLPATAMDVFQEQFLQ 440

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O644706.8e-10543.23Spermidine hydroxycinnamoyl transferase OS=Arabidopsis thaliana OX=3702 GN=SHT P... [more]
Q8GSM73.1e-8138.29Shikimate O-hydroxycinnamoyltransferase OS=Nicotiana tabacum OX=4097 GN=HST PE=1... [more]
Q9FI784.4e-8038.02Shikimate O-hydroxycinnamoyltransferase OS=Arabidopsis thaliana OX=3702 GN=HST P... [more]
A0A2H5AIZ13.9e-7636.13Hydroxycinnamoyltransferase OS=Narcissus pseudonarcissus OX=39639 GN=HCT PE=2 SV... [more]
A0PDV52.1e-7438.38Rosmarinate synthase OS=Plectranthus scutellarioides OX=4142 GN=RAS PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EEM21.4e-270100.00spermidine hydroxycinnamoyl transferase-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1EBK11.3e-26396.95spermidine hydroxycinnamoyl transferase-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1HNZ24.2e-25994.55spermidine hydroxycinnamoyl transferase-like isoform X2 OS=Cucurbita maxima OX=3... [more]
A0A6J1HTD77.9e-25894.35spermidine hydroxycinnamoyl transferase-like isoform X1 OS=Cucurbita maxima OX=3... [more]
A0A6J1EB804.8e-25593.70spermidine hydroxycinnamoyl transferase-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
XP_022925233.12.9e-270100.00spermidine hydroxycinnamoyl transferase-like [Cucurbita moschata] >KAG6595494.1 ... [more]
XP_023517444.13.0e-26798.69spermidine hydroxycinnamoyl transferase-like [Cucurbita pepo subsp. pepo][more]
KAG6595492.11.8e-26497.60Spermidine hydroxycinnamoyl transferase, partial [Cucurbita argyrosperma subsp. ... [more]
XP_023517487.16.9e-26497.60spermidine hydroxycinnamoyl transferase-like [Cucurbita pepo subsp. pepo][more]
XP_022925234.12.6e-26396.95spermidine hydroxycinnamoyl transferase-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT2G19070.14.8e-10643.23spermidine hydroxycinnamoyl transferase [more]
AT5G48930.13.1e-8138.02hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase [more]
AT5G57840.11.5e-5431.52HXXXD-type acyl-transferase family protein [more]
AT5G41040.14.1e-4932.30HXXXD-type acyl-transferase family protein [more]
AT5G41040.24.1e-4932.30HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 2..209
e-value: 6.9E-64
score: 217.3
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 215..458
e-value: 5.3E-62
score: 210.8
IPR003480TransferasePFAMPF02458Transferasecoord: 3..457
e-value: 4.0E-105
score: 352.0
NoneNo IPR availablePANTHERPTHR31642TRICHOTHECENE 3-O-ACETYLTRANSFERASEcoord: 6..459
NoneNo IPR availablePANTHERPTHR31642:SF57SPERMIDINE HYDROXYCINNAMOYL TRANSFERASEcoord: 6..459

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmoCh07G012040CmoCh07G012040gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh07G012040.1:exon:6970CmoCh07G012040.1:exon:6970exon
CmoCh07G012040.1:exon:6969CmoCh07G012040.1:exon:6969exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh07G012040.1:three_prime_utrCmoCh07G012040.1:three_prime_utrthree_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh07G012040.1:cdsCmoCh07G012040.1:cds_2CDS
CmoCh07G012040.1:cdsCmoCh07G012040.1:cdsCDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh07G012040.1:five_prime_utrCmoCh07G012040.1:five_prime_utrfive_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmoCh07G012040.1CmoCh07G012040.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups