Cmc08g0227131.1 (mRNA) Melon (Charmono) v1.1

Overview


Name	Cmc08g0227131.1
Type	mRNA
Organism	Cucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	aspartic proteinase nepenthesin-1
Location	CMiso1.1chr08: 19937946 .. 19940019 (-)
Sequence length	1729
RNA-Seq Expression	Cmc08g0227131.1
Synteny	Cmc08g0227131.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTGGTTTGCCTATCCTTTCCAAATTTCTCTCTCTACTTTCTTTAATTTCATTATATTAATATTCTCTTGTTTTGAAGAAGGGTTCAAGAACTTAACTCTTATTATTTGGCTCCCTCTTTTTAATTTCTATATTCTTTTCTTCGCATATGCTATACTATGGCGGCTGTCTCGTTACGTTCATTTGGATATCTGCAGCTTCTTTTGCTTATTGTATTCATTACAACATTATTTATTAATACATTGGCTTTCAGTTCATCTCTATCAACGCGAGCTCTGCAAAAACCAAATAAGTTGCCTAGTCATGGCTTCAGGGTGAGGCTTAAACATGTAGACCATGTGAAGAATTTGACGAGATTCGAGCGGTTGCGGCGTGGGGTGGCTCGAGGGAAAAATAGACTGCATAGACTAAATGCCATGGTGTTGGCCGCTGCCAATGCTTCGGTTGGAGACCAAGTGAAGGCACCAGTGGTTGCAGGTAATGGTGAGTTTCTTATGAAGTTGGCCATCGGAAGTCCGCCTAGAAGTTTCTCGGCGATCATGGATACAGGCAGCGATCTGATTTGGACACAATGCAAGCCTTGTCAACAGTGCTTCGATCAAGCAACACCTATTTTTGATCCCAAACAATCTTCTTCTTTCTCTAAGATTTCTTGCCGGAGCGAGCTCTGTGGAGCTCTCCCAACGTCAACATGCAGTAGCGACGGGTGCGAGTATTTGTACACATATGGAGATTCTTCCTCCACCCAAGGTGTTTTGGCTTTTGAGACCTTCACCTTTGGAGATTCAACTGAAGATCAGGTACTATTAATTTCCACATGAAAATATATAGACTTAAGAAATTTTGAAATTTCATTATTGAATAAAATAGATATTATTAATTTACTCACTCATGTTTTGAAATACAAGTATGTGAATAATAATAAAAAGACTAAATGTAGATGATTATCAAACAAGATTTAAATTCTAATATATTAAATTCTTTTAATATATTTTCTCAATCCAGATCTCGATCCCTGGACTCGGGTTTGGATGCGGAAACGATAACAATGGAGATGGGTTCAGCCAAGGCGCAGGGCTAGTGGGGCTTGGACGAGGACCCTTATCATTGGTTTCTCAACTAAAAGAACAAAAGTTTGCTTACTGTTTAACAGCCATTGATGATTCAAAACCAAGCTCACTTTTGTTAGGGTCTCTAGCAAACATAACACCTAAAACATCAAAGGATGAAATGAAAGCAACCCCATTGATCAAAAACCCTTCTCAACCATCTTTTTACTATCTTTCTCTCCAAGGAATCTCAGTTGGTGGAACTCAGTTATCAATACCAAAGTCCACTTTTGAGCTCCATGATGATGGGAGTGGAGGAGTAATCATAGACTCAGGCACAACAATCACTTACATTGAGAGCACAGCTTTCTCTTCACTCAAAAATGAGTTCATTGCTCAAATGAATCTTCCCGTTGACGATTCTGGTACTGGTGGTCTTGACCTCTGCTTTAACCTACCAGCAGGGACAACTCAGGTACGTCCACTTTGATATGTTACAATACACAACAATATATTAATACGAGTAAAATCACTGTTCCCATGTTTAATATATATGTTTCCAAAACACACCTTGATCATGTCATTGATCGTTTTGTTTACGTCTTTTTAACAAAGGTGGAGGTTCCGAAGTTGACGTTTCATTTCAAGGGCGCGGATTTGGAGCTGCCTGGGGAGAATTACATGATTGGGGATTCAAAGACGGGATTGCTATGCTTGGCCATTGGGAGTTCTAGAGGAATGTCCATCTTTGGAAATCTACAGCAACAAAATTTCATGGTTGTTCACGATCTTCAAGAAGAAACCCTATCTTTTTTGCCAACTCAATGTGATAGTATATAAAAAGAACTTGAAAGGAATTGTTTGATTACAATAGAGTGAAATGAAGGAAGAATATAAATGTTTGGAGTGAAATGATATATAAAAAGTTGTTGATTAAGCAGAAAAAAAATCAATATTATTTTTCATGAATCCATAGATCGTTCATCTTTATGATATTAATTCATGCAATTCAATTTATATGATTAT

mRNA sequence

GTTGGTTTGCCTATCCTTTCCAAATTTCTCTCTCTACTTTCTTTAATTTCATTATATTAATATTCTCTTGTTTTGAAGAAGGGTTCAAGAACTTAACTCTTATTATTTGGCTCCCTCTTTTTAATTTCTATATTCTTTTCTTCGCATATGCTATACTATGGCGGCTGTCTCGTTACGTTCATTTGGATATCTGCAGCTTCTTTTGCTTATTGTATTCATTACAACATTATTTATTAATACATTGGCTTTCAGTTCATCTCTATCAACGCGAGCTCTGCAAAAACCAAATAAGTTGCCTAGTCATGGCTTCAGGGTGAGGCTTAAACATGTAGACCATGTGAAGAATTTGACGAGATTCGAGCGGTTGCGGCGTGGGGTGGCTCGAGGGAAAAATAGACTGCATAGACTAAATGCCATGGTGTTGGCCGCTGCCAATGCTTCGGTTGGAGACCAAGTGAAGGCACCAGTGGTTGCAGGTAATGGTGAGTTTCTTATGAAGTTGGCCATCGGAAGTCCGCCTAGAAGTTTCTCGGCGATCATGGATACAGGCAGCGATCTGATTTGGACACAATGCAAGCCTTGTCAACAGTGCTTCGATCAAGCAACACCTATTTTTGATCCCAAACAATCTTCTTCTTTCTCTAAGATTTCTTGCCGGAGCGAGCTCTGTGGAGCTCTCCCAACGTCAACATGCAGTAGCGACGGGTGCGAGTATTTGTACACATATGGAGATTCTTCCTCCACCCAAGGTGTTTTGGCTTTTGAGACCTTCACCTTTGGAGATTCAACTGAAGATCAGATCTCGATCCCTGGACTCGGGTTTGGATGCGGAAACGATAACAATGGAGATGGGTTCAGCCAAGGCGCAGGGCTAGTGGGGCTTGGACGAGGACCCTTATCATTGGTTTCTCAACTAAAAGAACAAAAGTTTGCTTACTGTTTAACAGCCATTGATGATTCAAAACCAAGCTCACTTTTGTTAGGGTCTCTAGCAAACATAACACCTAAAACATCAAAGGATGAAATGAAAGCAACCCCATTGATCAAAAACCCTTCTCAACCATCTTTTTACTATCTTTCTCTCCAAGGAATCTCAGTTGGTGGAACTCAGTTATCAATACCAAAGTCCACTTTTGAGCTCCATGATGATGGGAGTGGAGGAGTAATCATAGACTCAGGCACAACAATCACTTACATTGAGAGCACAGCTTTCTCTTCACTCAAAAATGAGTTCATTGCTCAAATGAATCTTCCCGTTGACGATTCTGGTACTGGTGGTCTTGACCTCTGCTTTAACCTACCAGCAGGGACAACTCAGGTGGAGGTTCCGAAGTTGACGTTTCATTTCAAGGGCGCGGATTTGGAGCTGCCTGGGGAGAATTACATGATTGGGGATTCAAAGACGGGATTGCTATGCTTGGCCATTGGGAGTTCTAGAGGAATGTCCATCTTTGGAAATCTACAGCAACAAAATTTCATGGTTGTTCACGATCTTCAAGAAGAAACCCTATCTTTTTTGCCAACTCAATGTGATAGTATATAAAAAGAACTTGAAAGGAATTGTTTGATTACAATAGAGTGAAATGAAGGAAGAATATAAATGTTTGGAGTGAAATGATATATAAAAAGTTGTTGATTAAGCAGAAAAAAAATCAATATTATTTTTCATGAATCCATAGATCGTTCATCTTTATGATATTAATTCATGCAATTCAATTTATATGATTAT

Coding sequence (CDS)

ATGGCGGCTGTCTCGTTACGTTCATTTGGATATCTGCAGCTTCTTTTGCTTATTGTATTCATTACAACATTATTTATTAATACATTGGCTTTCAGTTCATCTCTATCAACGCGAGCTCTGCAAAAACCAAATAAGTTGCCTAGTCATGGCTTCAGGGTGAGGCTTAAACATGTAGACCATGTGAAGAATTTGACGAGATTCGAGCGGTTGCGGCGTGGGGTGGCTCGAGGGAAAAATAGACTGCATAGACTAAATGCCATGGTGTTGGCCGCTGCCAATGCTTCGGTTGGAGACCAAGTGAAGGCACCAGTGGTTGCAGGTAATGGTGAGTTTCTTATGAAGTTGGCCATCGGAAGTCCGCCTAGAAGTTTCTCGGCGATCATGGATACAGGCAGCGATCTGATTTGGACACAATGCAAGCCTTGTCAACAGTGCTTCGATCAAGCAACACCTATTTTTGATCCCAAACAATCTTCTTCTTTCTCTAAGATTTCTTGCCGGAGCGAGCTCTGTGGAGCTCTCCCAACGTCAACATGCAGTAGCGACGGGTGCGAGTATTTGTACACATATGGAGATTCTTCCTCCACCCAAGGTGTTTTGGCTTTTGAGACCTTCACCTTTGGAGATTCAACTGAAGATCAGATCTCGATCCCTGGACTCGGGTTTGGATGCGGAAACGATAACAATGGAGATGGGTTCAGCCAAGGCGCAGGGCTAGTGGGGCTTGGACGAGGACCCTTATCATTGGTTTCTCAACTAAAAGAACAAAAGTTTGCTTACTGTTTAACAGCCATTGATGATTCAAAACCAAGCTCACTTTTGTTAGGGTCTCTAGCAAACATAACACCTAAAACATCAAAGGATGAAATGAAAGCAACCCCATTGATCAAAAACCCTTCTCAACCATCTTTTTACTATCTTTCTCTCCAAGGAATCTCAGTTGGTGGAACTCAGTTATCAATACCAAAGTCCACTTTTGAGCTCCATGATGATGGGAGTGGAGGAGTAATCATAGACTCAGGCACAACAATCACTTACATTGAGAGCACAGCTTTCTCTTCACTCAAAAATGAGTTCATTGCTCAAATGAATCTTCCCGTTGACGATTCTGGTACTGGTGGTCTTGACCTCTGCTTTAACCTACCAGCAGGGACAACTCAGGTGGAGGTTCCGAAGTTGACGTTTCATTTCAAGGGCGCGGATTTGGAGCTGCCTGGGGAGAATTACATGATTGGGGATTCAAAGACGGGATTGCTATGCTTGGCCATTGGGAGTTCTAGAGGAATGTCCATCTTTGGAAATCTACAGCAACAAAATTTCATGGTTGTTCACGATCTTCAAGAAGAAACCCTATCTTTTTTGCCAACTCAATGTGATAGTATATAA

Protein sequence

MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDHVKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCSSDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI

Homology

[Close]

BLAST of Cmc08g0227131.1 vs. NCBI nr
Match: XP_008442220.1 (PREDICTED: aspartic proteinase nepenthesin-1 [Cucumis melo] >KAA0041170.1 aspartic proteinase nepenthesin-1 [Cucumis melo var. makuwa])

HSP 1 Score: 907.9 bits (2345), Expect = 3.5e-260
Identity = 461/461 (100.00%), Postives = 461/461 (100.00%), Query Frame = 0


Query: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDH 60
           MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDH
Sbjct: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDH 60

Query: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSP 120
           VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSP
Sbjct: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSP 120

Query: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCS 180
           PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCS
Sbjct: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCS 180

Query: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240
           SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV
Sbjct: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240

Query: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPS 300
           GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPS
Sbjct: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPS 300

Query: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFI 360
           QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFI
Sbjct: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFI 360

Query: 361 AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLC 420
           AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLC
Sbjct: 361 AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLC 420

Query: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 462
           LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of Cmc08g0227131.1 vs. NCBI nr
Match: TYK02448.1 (aspartic proteinase nepenthesin-1 [Cucumis melo var. makuwa])

HSP 1 Score: 903.3 bits (2333), Expect = 8.7e-259
Identity = 459/461 (99.57%), Postives = 459/461 (99.57%), Query Frame = 0


Query: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDH 60
           MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLS RALQKPNKLPSHGF VRLKHVDH
Sbjct: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSRRALQKPNKLPSHGFMVRLKHVDH 60

Query: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSP 120
           VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSP
Sbjct: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSP 120

Query: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCS 180
           PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCS
Sbjct: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCS 180

Query: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240
           SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV
Sbjct: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240

Query: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPS 300
           GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPS
Sbjct: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPS 300

Query: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFI 360
           QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFI
Sbjct: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFI 360

Query: 361 AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLC 420
           AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLC
Sbjct: 361 AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLC 420

Query: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 462
           LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of Cmc08g0227131.1 vs. NCBI nr
Match: XP_011653928.1 (aspartic proteinase nepenthesin-1 [Cucumis sativus] >KGN54860.1 hypothetical protein Csa_012038 [Cucumis sativus])

HSP 1 Score: 882.1 bits (2278), Expect = 2.1e-252
Identity = 445/461 (96.53%), Postives = 452/461 (98.05%), Query Frame = 0


Query: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDH 60
           MAAVSLRSFGYL  LLLI+ ITTLFINTLAFSSSLS RALQKPNKLPSHGFRVRLKHVDH
Sbjct: 1   MAAVSLRSFGYLHRLLLIILITTLFINTLAFSSSLSRRALQKPNKLPSHGFRVRLKHVDH 60

Query: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSP 120
           VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANA+VGDQVKAPVVAGNGEFLMKLAIGSP
Sbjct: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSP 120

Query: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCS 180
           PRSFSAIMDTGSDLIWTQCKPCQQCFDQ+TPIFDPKQSSSF KISC SELCGALPTSTCS
Sbjct: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDPKQSSSFYKISCSSELCGALPTSTCS 180

Query: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240
           SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV
Sbjct: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240

Query: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPS 300
           GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMK TPLIKNPS
Sbjct: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPS 300

Query: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFI 360
           QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITY+E++AF+SLKNEFI
Sbjct: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYVENSAFTSLKNEFI 360

Query: 361 AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLC 420
           AQMNLPVDDSGTGGLDLCFNLPAGT QVEVPKLTFHFKGADLELPGENYMIGDSK GLLC
Sbjct: 361 AQMNLPVDDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLC 420

Query: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 462
           LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of Cmc08g0227131.1 vs. NCBI nr
Match: XP_038883313.1 (aspartic proteinase nepenthesin-1 [Benincasa hispida])

HSP 1 Score: 799.7 bits (2064), Expect = 1.4e-227
Identity = 408/458 (89.08%), Postives = 433/458 (94.54%), Query Frame = 0


Query: 4   VSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDHVKN 63
           VSL S+  L +LL+IV ITTLFI+TLA    LS RALQK N+LPS+GF+V+L HVDHVKN
Sbjct: 3   VSLHSYRCL-ILLVIVLITTLFIDTLA----LSRRALQKSNELPSNGFKVKLNHVDHVKN 62

Query: 64  LTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSPPRS 123
           LTRFERLRRGVARGKNRLHRLNAMVL AANA+VG+QV+APVVAGNGEFLMKLAIG+PP+S
Sbjct: 63  LTRFERLRRGVARGKNRLHRLNAMVL-AANAAVGEQVQAPVVAGNGEFLMKLAIGTPPKS 122

Query: 124 FSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCSSDG 183
           FSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPK SSSFSKISC SELCG LPTSTCSSDG
Sbjct: 123 FSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKASSSFSKISCSSELCGPLPTSTCSSDG 182

Query: 184 CEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGLG 243
           CEYLYTYGDSSSTQGVLA ETFTFGDS++DQ+SI GLGFGCG+DN GDGFSQGAGLVGLG
Sbjct: 183 CEYLYTYGDSSSTQGVLALETFTFGDSSDDQVSITGLGFGCGDDNEGDGFSQGAGLVGLG 242

Query: 244 RGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPSQPS 303
           RGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANI PKT+KDEMK TPLI+NPSQPS
Sbjct: 243 RGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANIKPKTTKDEMKTTPLIRNPSQPS 302

Query: 304 FYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFIAQM 363
           FYYLSLQGISVG TQLSIPK+TFELHDDGSGGVIIDSGTTITYIE+TAF+ LKNEFIAQM
Sbjct: 303 FYYLSLQGISVGSTQLSIPKTTFELHDDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQM 362

Query: 364 NLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLCLAI 423
           NLPVDDSGTGGLDLCFNLPA  TQVEVPKLTFHF+GADLELPGENYMIGDS+TGL+CLAI
Sbjct: 363 NLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFEGADLELPGENYMIGDSRTGLICLAI 422

Query: 424 GSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 462
           GSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 423 GSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 454

[Close]

BLAST of Cmc08g0227131.1 vs. NCBI nr
Match: XP_022928703.1 (aspartic proteinase nepenthesin-1 [Cucurbita moschata])

HSP 1 Score: 743.4 bits (1918), Expect = 1.2e-210
Identity = 379/454 (83.48%), Postives = 407/454 (89.65%), Query Frame = 0


Query: 8   SFGYLQLLLLIVFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDHVKNLTRF 67
           S  YL ++ +++ IT LFI+T A SSSLS RAL +P KLPS GFRV L HVDHVKNLTRF
Sbjct: 4   SLRYL-IISVVLSITMLFIHTSASSSSLSRRALWQP-KLPSDGFRVSLNHVDHVKNLTRF 63

Query: 68  ERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSPPRSFSAI 127
           ERL+RGVARGK RLHRLNAMVL AAN  VG +V+APVVAGNGEFLMKLAIGSPPRSFSAI
Sbjct: 64  ERLQRGVARGKTRLHRLNAMVL-AANVGVGGRVQAPVVAGNGEFLMKLAIGSPPRSFSAI 123

Query: 128 MDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCSSDGCEYL 187
           MDTGSDLIWTQCKPCQQCFDQATPIFDPK+SSSFSKISC SELC ALPTSTCSSD CEY 
Sbjct: 124 MDTGSDLIWTQCKPCQQCFDQATPIFDPKESSSFSKISCSSELCDALPTSTCSSDECEYF 183

Query: 188 YTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGLGRGPL 247
           YTYGD SST GVLA ETFTFGDS++DQ+SIPGLGFGCG+DN GDGFSQG GLVGLGRGPL
Sbjct: 184 YTYGDYSSTHGVLAAETFTFGDSSQDQVSIPGLGFGCGDDNEGDGFSQGEGLVGLGRGPL 243

Query: 248 SLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPSQPSFYYL 307
           SLVSQLKEQKF+YCLTAIDD+KPSSLL+GSLAN+ PK S+ E+K TPLI+NPSQPSFYYL
Sbjct: 244 SLVSQLKEQKFSYCLTAIDDTKPSSLLMGSLANVKPKASEGEIKTTPLIRNPSQPSFYYL 303

Query: 308 SLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFIAQMNLPV 367
           SLQGISVGGTQL IPK+TFELHDDGSGGVIIDSGTTITYIE  AF+ LK EF++QM LPV
Sbjct: 304 SLQGISVGGTQLPIPKATFELHDDGSGGVIIDSGTTITYIEKNAFTLLKKEFVSQMKLPV 363

Query: 368 DDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLCLAIGSSR 427
           DDSGT GLDLCFNLP  TTQVEVPKLTFHFKGADLELPGENYMIGDSK  L+CLAIGSS 
Sbjct: 364 DDSGTSGLDLCFNLPPETTQVEVPKLTFHFKGADLELPGENYMIGDSKAELICLAIGSSS 423

Query: 428 GMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 462
           GMSIFGNLQQQN MVVHDLQEET+SFLPTQC  I
Sbjct: 424 GMSIFGNLQQQNIMVVHDLQEETVSFLPTQCSEI 454

[Close]

BLAST of Cmc08g0227131.1 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 2.0e-117
Identity = 231/449 (51.45%), Postives = 303/449 (67.48%), Query Frame = 0


Query: 12  LQLLLLIVFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDHVKNLTRFERLR 71
           L   LL + I  +F+   A + S S  AL   ++    GF++ L+HVD  KNLT+F+ L 
Sbjct: 5   LYSFLLALSIVYIFV---APTHSTSRTALNHRHEAKVTGFQIMLEHVDSGKNLTKFQLLE 64

Query: 72  RGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSPPRSFSAIMDTG 131
           R + RG  RL RL AM+    N   G  V+  V AG+GE+LM L+IG+P + FSAIMDTG
Sbjct: 65  RAIERGSRRLQRLEAML----NGPSG--VETSVYAGDGEYLMNLSIGTPAQPFSAIMDTG 124

Query: 132 SDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCSSDGCEYLYTYG 191
           SDLIWTQC+PC QCF+Q+TPIF+P+ SSSFS + C S+LC AL + TCS++ C+Y Y YG
Sbjct: 125 SDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLCQALSSPTCSNNFCQYTYGYG 184

Query: 192 DSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGLGRGPLSLVS 251
           D S TQG +  ET TFG      +SIP + FGCG +N G G   GAGLVG+GRGPLSL S
Sbjct: 185 DGSETQGSMGTETLTFG-----SVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPS 244

Query: 252 QLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPSQPSFYYLSLQG 311
           QL   KF+YC+T I  S PS+LLLGSLAN     S +    T LI++   P+FYY++L G
Sbjct: 245 QLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPN----TTLIQSSQIPTFYYITLNG 304

Query: 312 ISVGGTQLSIPKSTFELH-DDGSGGVIIDSGTTITYIESTAFSSLKNEFIAQMNLPVDDS 371
           +SVG T+L I  S F L+ ++G+GG+IIDSGTT+TY  + A+ S++ EFI+Q+NLPV + 
Sbjct: 305 LSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNG 364

Query: 372 GTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLCLAIG-SSRGM 431
            + G DLCF  P+  + +++P    HF G DLELP ENY I  S  GL+CLA+G SS+GM
Sbjct: 365 SSSGFDLCFQTPSDPSNLQIPTFVMHFDGGDLELPSENYFISPS-NGLICLAMGSSSQGM 424

Query: 432 SIFGNLQQQNFMVVHDLQEETLSFLPTQC 459
           SIFGN+QQQN +VV+D     +SF   QC
Sbjct: 425 SIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of Cmc08g0227131.1 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 1.0e-113
Identity = 211/429 (49.18%), Postives = 291/429 (67.83%), Query Frame = 0


Query: 32  SSSLSTRALQKPNKLPSHGFRVRLKHVDHVKNLTRFERLRRGVARGKNRLHRLNAMVLAA 91
           SS+     L    K P  G RV L+ VD  KNLT++E ++R + RG+ R+  +NAM+ ++
Sbjct: 23  SSTSRGTLLHHGQKRPQPGLRVDLEQVDSGKNLTKYELIKRAIKRGERRMRSINAMLQSS 82

Query: 92  ANASVGDQVKAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCKPCQQCFDQATP 151
           +       ++ PV AG+GE+LM +AIG+P  SFSAIMDTGSDLIWTQC+PC QCF Q TP
Sbjct: 83  SG------IETPVYAGDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTP 142

Query: 152 IFDPKQSSSFSKISCRSELCGALPTSTCSSDGCEYLYTYGDSSSTQGVLAFETFTFGDST 211
           IF+P+ SSSFS + C S+ C  LP+ TC+++ C+Y Y YGD S+TQG +A ETFTF  S 
Sbjct: 143 IFNPQDSSSFSTLPCESQYCQDLPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETS- 202

Query: 212 EDQISIPGLGFGCGNDNNGDGFSQGAGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPS 271
               S+P + FGCG DN G G   GAGL+G+G GPLSL SQL   +F+YC+T+   S PS
Sbjct: 203 ----SVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPS 262

Query: 272 SLLLGSLANITPKTSKDEMKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDD 331
           +L LGS A+  P+ S     +T LI +   P++YY++LQGI+VGG  L IP STF+L DD
Sbjct: 263 TLALGSAASGVPEGS----PSTTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQLQDD 322

Query: 332 GSGGVIIDSGTTITYIESTAFSSLKNEFIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVP 391
           G+GG+IIDSGTT+TY+   A++++   F  Q+NLP  D  + GL  CF  P+  + V+VP
Sbjct: 323 GTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCFQQPSDGSTVQVP 382

Query: 392 KLTFHFKGADLELPGENYMIGDSKTGLLCLAIGSSR--GMSIFGNLQQQNFMVVHDLQEE 451
           +++  F G  L L  +N +I  ++ G++CLA+GSS   G+SIFGN+QQQ   V++DLQ  
Sbjct: 383 EISMQFDGGVLNLGEQNILISPAE-GVICLAMGSSSQLGISIFGNIQQQETQVLYDLQNL 435

Query: 452 TLSFLPTQC 459
            +SF+PTQC
Sbjct: 443 AVSFVPTQC 435

[Close]

BLAST of Cmc08g0227131.1 vs. ExPASy Swiss-Prot
Match: Q7XV21 (Aspartyl protease 37 OS=Oryza sativa subsp. japonica OX=39947 GN=AP37 PE=3 SV=2)

HSP 1 Score: 268.5 bits (685), Expect = 1.4e-70
Identity = 157/450 (34.89%), Postives = 239/450 (53.11%), Query Frame = 0


Query: 47  PSHGFRVRLKHVD----HVKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKA 106
           P   FR+ L  VD       NLT  E LRR + R + RL  +      AA+A      + 
Sbjct: 21  PPRSFRLELASVDASAADAANLTEHELLRRAIQRSRYRLAGIGMARGEAASARKAVVAET 80

Query: 107 PVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFS 166
           P++   GE+L+KL IG+PP  F+A +DT SDLIWTQC+PC  C+ Q  P+F+P+ SS+++
Sbjct: 81  PIMPAGGEYLVKLGIGTPPYKFTAAIDTASDLIWTQCQPCTGCYHQVDPMFNPRVSSTYA 140

Query: 167 KISCRSELCGALPTSTCSSD---GCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPG 226
            + C S+ C  L    C  D    C+Y YTY  +++T+G LA +    G+      +  G
Sbjct: 141 ALPCSSDTCDELDVHRCGHDDDESCQYTYTYSGNATTEGTLAVDKLVIGED-----AFRG 200

Query: 227 LGFGCGNDNNGDG-FSQGAGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSL 286
           + FGC   + G     Q +G+VGLGRGPLSLVSQL  ++FAYCL       P  L+LG+ 
Sbjct: 201 VAFGCSTSSTGGAPPPQASGVVGLGRGPLSLVSQLSVRRFAYCLPPPASRIPGKLVLGAD 260

Query: 287 ANITPKTSKDEMKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKST------------- 346
           A+     +     A P+ ++P  PS+YYL+L G+ +G   +S+P +T             
Sbjct: 261 ADAARNAT--NRIAVPMRRDPRYPSYYYLNLDGLLIGDRAMSLPPTTTTTATATATAPAP 320

Query: 347 ----------FELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFIAQMNLPVDDSGTGGL 406
                       + D    G+IID  +TIT++E++ +  L N+   ++ LP     + GL
Sbjct: 321 APTPSPNATAVAVGDANRYGMIIDIASTITFLEASLYDELVNDLEVEIRLPRGTGSSLGL 380

Query: 407 DLCFNLPAGTT--QVEVPKLTFHFKGADLELPGENYMIGDSKTGLLCLAIGSSR--GMSI 462
           DLCF LP G    +V VP +   F G  L L        D ++G++CL +G +    +SI
Sbjct: 381 DLCFILPDGVAFDRVYVPAVALAFDGRWLRLDKARLFAEDRESGMMCLMVGRAEAGSVSI 440

[Close]

BLAST of Cmc08g0227131.1 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 2.4e-70
Identity = 161/444 (36.26%), Postives = 240/444 (54.05%), Query Frame = 0


Query: 21  ITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDHVKNLTRFERLRRGVARGKNR 80
           + +LF + L     LS+  L   N  P  GF   L H D  K+   +  +     R +N 
Sbjct: 1   MASLFSSVLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKS-PFYNPMETSSQRLRNA 60

Query: 81  LHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCK 140
           +HR    V          Q +  + + +GE+LM ++IG+PP    AI DTGSDL+WTQC 
Sbjct: 61  IHRSVNRVFHFTEKDNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCA 120

Query: 141 PCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPT-STCSSDG--CEYLYTYGDSSSTQ 200
           PC  C+ Q  P+FDPK SS++  +SC S  C AL   ++CS++   C Y  +YGD+S T+
Sbjct: 121 PCDDCYTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTK 180

Query: 201 GVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGLGRGPLSLVSQLKEQ- 260
           G +A +T T G S    + +  +  GCG++N G    +G+G+VGLG GP+SL+ QL +  
Sbjct: 181 GNIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSI 240

Query: 261 --KFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPSQPSFYYLSLQGISV 320
             KF+YCL  +   K  +  +    N     S   + +TPLI   SQ +FYYL+L+ ISV
Sbjct: 241 DGKFSYCLVPLTSKKDQTSKINFGTNAI--VSGSGVVSTPLIAKASQETFYYLTLKSISV 300

Query: 321 GGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFIAQMNLPVDDSGTGG 380
           G  Q+    S  E      G +IIDSGTT+T + +  +S L++   + ++         G
Sbjct: 301 GSKQIQYSGSDSE---SSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSG 360

Query: 381 LDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLCLAIGSSRGMSIFGN 440
           L LC+   + T  ++VP +T HF GAD++L   N  +  S+  L+C A   S   SI+GN
Sbjct: 361 LSLCY---SATGDLKVPVITMHFDGADVKLDSSNAFVQVSE-DLVCFAFRGSPSFSIYGN 420

Query: 441 LQQQNFMVVHDLQEETLSFLPTQC 459
           + Q NF+V +D   +T+SF PT C
Sbjct: 421 VAQMNFLVGYDTVSKTVSFKPTDC 434

[Close]

BLAST of Cmc08g0227131.1 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 7.3e-67
Identity = 138/368 (37.50%), Postives = 216/368 (58.70%), Query Frame = 0


Query: 98  DQVKAPVVA----GNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIF 157
           + +  PVV+    G+GE+  ++ +G+P +    ++DTGSD+ W QC+PC  C+ Q+ P+F
Sbjct: 145 EDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVF 204

Query: 158 DPKQSSSFSKISCRSELCGALPTSTCSSDGCEYLYTYGDSSSTQGVLAFETFTFGDSTED 217
           +P  SS++  ++C +  C  L TS C S+ C Y  +YGD S T G LA +T TFG+S + 
Sbjct: 205 NPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGK- 264

Query: 218 QISIPGLGFGCGNDNNGDGFSQGAGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSL 277
              I  +  GCG+DN G  F+  AGL+GLG G LS+ +Q+K   F+YCL   D  K SSL
Sbjct: 265 ---INNVALGCGHDNEG-LFTGAAGLLGLGGGVLSITNQMKATSFSYCLVDRDSGKSSSL 324

Query: 278 LLGSLANITPKTSKDEMKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGS 337
              S+     +    +  A PL++N    +FYY+ L G SVGG ++ +P + F++   GS
Sbjct: 325 DFNSV-----QLGGGDATA-PLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGS 384

Query: 338 GGVIIDSGTTITYIESTAFSSLKNEFI-AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPK 397
           GGVI+D GT +T +++ A++SL++ F+   +NL    S     D C++  + +T V+VP 
Sbjct: 385 GGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLST-VKVPT 444

Query: 398 LTFHFKGA-DLELPGENYMIGDSKTGLLCLAIG-SSRGMSIFGNLQQQNFMVVHDLQEET 457
           + FHF G   L+LP +NY+I    +G  C A   +S  +SI GN+QQQ   + +DL +  
Sbjct: 445 VAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNV 500

Query: 458 LSFLPTQC 459
           +     +C
Sbjct: 505 IGLSGNKC 500

[Close]

BLAST of Cmc08g0227131.1 vs. ExPASy TrEMBL
Match: A0A5A7TD10 (Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold128G001020 PE=3 SV=1)

HSP 1 Score: 907.9 bits (2345), Expect = 1.7e-260
Identity = 461/461 (100.00%), Postives = 461/461 (100.00%), Query Frame = 0


Query: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDH 60
           MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDH
Sbjct: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDH 60

Query: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSP 120
           VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSP
Sbjct: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSP 120

Query: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCS 180
           PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCS
Sbjct: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCS 180

Query: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240
           SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV
Sbjct: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240

Query: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPS 300
           GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPS
Sbjct: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPS 300

Query: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFI 360
           QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFI
Sbjct: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFI 360

Query: 361 AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLC 420
           AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLC
Sbjct: 361 AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLC 420

Query: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 462
           LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of Cmc08g0227131.1 vs. ExPASy TrEMBL
Match: A0A1S3B573 (aspartic proteinase nepenthesin-1 OS=Cucumis melo OX=3656 GN=LOC103486136 PE=3 SV=1)

HSP 1 Score: 907.9 bits (2345), Expect = 1.7e-260
Identity = 461/461 (100.00%), Postives = 461/461 (100.00%), Query Frame = 0


Query: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDH 60
           MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDH
Sbjct: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDH 60

Query: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSP 120
           VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSP
Sbjct: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSP 120

Query: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCS 180
           PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCS
Sbjct: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCS 180

Query: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240
           SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV
Sbjct: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240

Query: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPS 300
           GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPS
Sbjct: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPS 300

Query: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFI 360
           QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFI
Sbjct: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFI 360

Query: 361 AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLC 420
           AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLC
Sbjct: 361 AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLC 420

Query: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 462
           LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of Cmc08g0227131.1 vs. ExPASy TrEMBL
Match: A0A5D3BTY9 (Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1738G00580 PE=3 SV=1)

HSP 1 Score: 903.3 bits (2333), Expect = 4.2e-259
Identity = 459/461 (99.57%), Postives = 459/461 (99.57%), Query Frame = 0


Query: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDH 60
           MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLS RALQKPNKLPSHGF VRLKHVDH
Sbjct: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSRRALQKPNKLPSHGFMVRLKHVDH 60

Query: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSP 120
           VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSP
Sbjct: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSP 120

Query: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCS 180
           PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCS
Sbjct: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCS 180

Query: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240
           SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV
Sbjct: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240

Query: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPS 300
           GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPS
Sbjct: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPS 300

Query: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFI 360
           QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFI
Sbjct: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFI 360

Query: 361 AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLC 420
           AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLC
Sbjct: 361 AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLC 420

Query: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 462
           LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of Cmc08g0227131.1 vs. ExPASy TrEMBL
Match: A0A0A0KYT9 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G554680 PE=3 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 1.0e-252
Identity = 445/461 (96.53%), Postives = 452/461 (98.05%), Query Frame = 0


Query: 1   MAAVSLRSFGYLQLLLLIVFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDH 60
           MAAVSLRSFGYL  LLLI+ ITTLFINTLAFSSSLS RALQKPNKLPSHGFRVRLKHVDH
Sbjct: 1   MAAVSLRSFGYLHRLLLIILITTLFINTLAFSSSLSRRALQKPNKLPSHGFRVRLKHVDH 60

Query: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSP 120
           VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANA+VGDQVKAPVVAGNGEFLMKLAIGSP
Sbjct: 61  VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSP 120

Query: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCS 180
           PRSFSAIMDTGSDLIWTQCKPCQQCFDQ+TPIFDPKQSSSF KISC SELCGALPTSTCS
Sbjct: 121 PRSFSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDPKQSSSFYKISCSSELCGALPTSTCS 180

Query: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240
           SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV
Sbjct: 181 SDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLV 240

Query: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPS 300
           GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMK TPLIKNPS
Sbjct: 241 GLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPS 300

Query: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFI 360
           QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITY+E++AF+SLKNEFI
Sbjct: 301 QPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYVENSAFTSLKNEFI 360

Query: 361 AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLC 420
           AQMNLPVDDSGTGGLDLCFNLPAGT QVEVPKLTFHFKGADLELPGENYMIGDSK GLLC
Sbjct: 361 AQMNLPVDDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLC 420

Query: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 462
           LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 421 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of Cmc08g0227131.1 vs. ExPASy TrEMBL
Match: A0A6J1EPU6 (aspartic proteinase nepenthesin-1 OS=Cucurbita moschata OX=3662 GN=LOC111435534 PE=3 SV=1)

HSP 1 Score: 743.4 bits (1918), Expect = 5.6e-211
Identity = 379/454 (83.48%), Postives = 407/454 (89.65%), Query Frame = 0


Query: 8   SFGYLQLLLLIVFITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDHVKNLTRF 67
           S  YL ++ +++ IT LFI+T A SSSLS RAL +P KLPS GFRV L HVDHVKNLTRF
Sbjct: 4   SLRYL-IISVVLSITMLFIHTSASSSSLSRRALWQP-KLPSDGFRVSLNHVDHVKNLTRF 63

Query: 68  ERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSPPRSFSAI 127
           ERL+RGVARGK RLHRLNAMVL AAN  VG +V+APVVAGNGEFLMKLAIGSPPRSFSAI
Sbjct: 64  ERLQRGVARGKTRLHRLNAMVL-AANVGVGGRVQAPVVAGNGEFLMKLAIGSPPRSFSAI 123

Query: 128 MDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCSSDGCEYL 187
           MDTGSDLIWTQCKPCQQCFDQATPIFDPK+SSSFSKISC SELC ALPTSTCSSD CEY 
Sbjct: 124 MDTGSDLIWTQCKPCQQCFDQATPIFDPKESSSFSKISCSSELCDALPTSTCSSDECEYF 183

Query: 188 YTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGLGRGPL 247
           YTYGD SST GVLA ETFTFGDS++DQ+SIPGLGFGCG+DN GDGFSQG GLVGLGRGPL
Sbjct: 184 YTYGDYSSTHGVLAAETFTFGDSSQDQVSIPGLGFGCGDDNEGDGFSQGEGLVGLGRGPL 243

Query: 248 SLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPSQPSFYYL 307
           SLVSQLKEQKF+YCLTAIDD+KPSSLL+GSLAN+ PK S+ E+K TPLI+NPSQPSFYYL
Sbjct: 244 SLVSQLKEQKFSYCLTAIDDTKPSSLLMGSLANVKPKASEGEIKTTPLIRNPSQPSFYYL 303

Query: 308 SLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFIAQMNLPV 367
           SLQGISVGGTQL IPK+TFELHDDGSGGVIIDSGTTITYIE  AF+ LK EF++QM LPV
Sbjct: 304 SLQGISVGGTQLPIPKATFELHDDGSGGVIIDSGTTITYIEKNAFTLLKKEFVSQMKLPV 363

Query: 368 DDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLCLAIGSSR 427
           DDSGT GLDLCFNLP  TTQVEVPKLTFHFKGADLELPGENYMIGDSK  L+CLAIGSS 
Sbjct: 364 DDSGTSGLDLCFNLPPETTQVEVPKLTFHFKGADLELPGENYMIGDSKAELICLAIGSSS 423

Query: 428 GMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 462
           GMSIFGNLQQQN MVVHDLQEET+SFLPTQC  I
Sbjct: 424 GMSIFGNLQQQNIMVVHDLQEETVSFLPTQCSEI 454

[Close]

BLAST of Cmc08g0227131.1 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 540.0 bits (1390), Expect = 1.8e-153
Identity = 281/459 (61.22%), Postives = 349/459 (76.03%), Query Frame = 0


Query: 16  LLIVFITTLFINTLAFSS---SLSTRALQKPNKLPSHGFRVRLKHVDHVKNLTRFERLRR 75
           LL  F   LF   ++ SS   SL  R L  P  LP  GFR+ L+HVD  KNLT+ ++++R
Sbjct: 9   LLFPFFLILFSCLISVSSSRRSLIDRTL--PKNLPRSGFRLSLRHVDSGKNLTKIQKIQR 68

Query: 76  GVARGKNRLHRLNAMVLAAANASVGD--QVKAPVVAGNGEFLMKLAIGSPPRSFSAIMDT 135
           G+ RG +RL+RL A+ + A  +   D   +KAP   G+GEFLM+L+IG+P   +SAI+DT
Sbjct: 69  GINRGFHRLNRLGAVAVLAVASKPDDTNNIKAPTHGGSGEFLMELSIGNPAVKYSAIVDT 128

Query: 136 GSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCS--SDGCEYLY 195
           GSDLIWTQCKPC +CFDQ TPIFDP++SSS+SK+ C S LC ALP S C+   D CEYLY
Sbjct: 129 GSDLIWTQCKPCTECFDQPTPIFDPEKSSSYSKVGCSSGLCNALPRSNCNEDKDACEYLY 188

Query: 196 TYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGLGRGPLS 255
           TYGD SST+G+LA ETFTF    ED+ SI G+GFGCG +N GDGFSQG+GLVGLGRGPLS
Sbjct: 189 TYGDYSSTRGLLATETFTF----EDENSISGIGFGCGVENEGDGFSQGSGLVGLGRGPLS 248

Query: 256 LVSQLKEQKFAYCLTAIDDSK-PSSLLLGSLAN-ITPKTSK----DEMKATPLIKNPSQP 315
           L+SQLKE KF+YCLT+I+DS+  SSL +GSLA+ I  KT      +  K   L++NP QP
Sbjct: 249 LISQLKETKFSYCLTSIEDSEASSSLFIGSLASGIVNKTGASLDGEVTKTMSLLRNPDQP 308

Query: 316 SFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFIAQ 375
           SFYYL LQGI+VG  +LS+ KSTFEL +DG+GG+IIDSGTTITY+E TAF  LK EF ++
Sbjct: 309 SFYYLELQGITVGAKRLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSR 368

Query: 376 MNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLCLA 435
           M+LPVDDSG+ GLDLCF LP     + VPK+ FHFKGADLELPGENYM+ DS TG+LCLA
Sbjct: 369 MSLPVDDSGSTGLDLCFKLPDAAKNIAVPKMIFHFKGADLELPGENYMVADSSTGVLCLA 428

Query: 436 IGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 462
           +GSS GMSIFGN+QQQNF V+HDL++ET+SF+PT+C  +
Sbjct: 429 MGSSNGMSIFGNVQQQNFNVLHDLEKETVSFVPTECGKL 461

[Close]

BLAST of Cmc08g0227131.1 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 267.7 bits (683), Expect = 1.7e-71
Identity = 165/428 (38.55%), Postives = 234/428 (54.67%), Query Frame = 0


Query: 52  RVRLKHVDH--VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGD----------- 111
           RV ++  +H   K+LT   RL R  AR K+ + RL+   LA  N S  D           
Sbjct: 74  RVSVRGTEHSDYKSLT-LARLNRDTARVKSLITRLD---LAINNISKADLKPISTMYTTE 133

Query: 112 --QVKAPVVA----GNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCKPCQQCFDQATPI 171
              ++AP+++    G+GE+  ++ IG P R    ++DTGSD+ W QC PC  C+ Q  PI
Sbjct: 134 EQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTPCADCYHQTEPI 193

Query: 172 FDPKQSSSFSKISCRSELCGALPTSTCSSDGCEYLYTYGDSSSTQGVLAFETFTFGDSTE 231
           F+P  SSS+  +SC +  C AL  S C +  C Y  +YGD S T G  A ET T G +  
Sbjct: 194 FEPSSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGDGSYTVGDFATETLTIGSTLV 253

Query: 232 DQISIPGLGFGCGNDNNGDGFSQGAGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSS 291
             +++     GCG+ N G  F   AGL+GLG G L+L SQL    F+YCL   D    S+
Sbjct: 254 QNVAV-----GCGHSNEG-LFVGAAGLLGLGGGLLALPSQLNTTSFSYCLVDRDSDSAST 313

Query: 292 LLLGSLANITPKTSKDEMKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDG 351
           +  G+  +++P     +    PL++N    +FYYL L GISVGG  L IP+S+FE+ + G
Sbjct: 314 VDFGT--SLSP-----DAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMDESG 373

Query: 352 SGGVIIDSGTTITYIESTAFSSLKNEFIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPK 411
           SGG+IIDSGT +T +++  ++SL++ F+         +G    D C+NL A TT VEVP 
Sbjct: 374 SGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTT-VEVPT 433

Query: 412 LTFHFKGAD-LELPGENYMIGDSKTGLLCLAIG-SSRGMSIFGNLQQQNFMVVHDLQEET 459
           + FHF G   L LP +NYMI     G  CLA   ++  ++I GN+QQQ   V  DL    
Sbjct: 434 VAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANSL 483

[Close]

BLAST of Cmc08g0227131.1 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 267.7 bits (683), Expect = 1.7e-71
Identity = 161/444 (36.26%), Postives = 240/444 (54.05%), Query Frame = 0


Query: 21  ITTLFINTLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDHVKNLTRFERLRRGVARGKNR 80
           + +LF + L     LS+  L   N  P  GF   L H D  K+   +  +     R +N 
Sbjct: 1   MASLFSSVLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKS-PFYNPMETSSQRLRNA 60

Query: 81  LHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCK 140
           +HR    V          Q +  + + +GE+LM ++IG+PP    AI DTGSDL+WTQC 
Sbjct: 61  IHRSVNRVFHFTEKDNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCA 120

Query: 141 PCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPT-STCSSDG--CEYLYTYGDSSSTQ 200
           PC  C+ Q  P+FDPK SS++  +SC S  C AL   ++CS++   C Y  +YGD+S T+
Sbjct: 121 PCDDCYTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTK 180

Query: 201 GVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGLGRGPLSLVSQLKEQ- 260
           G +A +T T G S    + +  +  GCG++N G    +G+G+VGLG GP+SL+ QL +  
Sbjct: 181 GNIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSI 240

Query: 261 --KFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPSQPSFYYLSLQGISV 320
             KF+YCL  +   K  +  +    N     S   + +TPLI   SQ +FYYL+L+ ISV
Sbjct: 241 DGKFSYCLVPLTSKKDQTSKINFGTNAI--VSGSGVVSTPLIAKASQETFYYLTLKSISV 300

Query: 321 GGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFIAQMNLPVDDSGTGG 380
           G  Q+    S  E      G +IIDSGTT+T + +  +S L++   + ++         G
Sbjct: 301 GSKQIQYSGSDSE---SSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSG 360

Query: 381 LDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLCLAIGSSRGMSIFGN 440
           L LC+   + T  ++VP +T HF GAD++L   N  +  S+  L+C A   S   SI+GN
Sbjct: 361 LSLCY---SATGDLKVPVITMHFDGADVKLDSSNAFVQVSE-DLVCFAFRGSPSFSIYGN 420

Query: 441 LQQQNFMVVHDLQEETLSFLPTQC 459
           + Q NF+V +D   +T+SF PT C
Sbjct: 421 VAQMNFLVGYDTVSKTVSFKPTDC 434

[Close]

BLAST of Cmc08g0227131.1 vs. TAIR 10
Match: AT1G64830.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 257.7 bits (657), Expect = 1.8e-68
Identity = 155/442 (35.07%), Postives = 238/442 (53.85%), Query Frame = 0


Query: 28  TLAFSSSLSTRALQKPNKLPSHGFRVRLKHVDHVKNLTRFERLRRGVARGKNRLHRLNAM 87
           +L F++ LS   L   N  P  GF + L H D  K+   +        R +N + R    
Sbjct: 3   SLIFATLLSLLLLSNVNAYPKDGFTIDLIHRDSPKS-PFYNSAETSSQRMRNAIRRSARS 62

Query: 88  VLAAANASVGDQVKAPVVAGN-GEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCKPCQQCF 147
            L  +N           +  N GE+LM ++IG+PP    AI DTGSDLIWTQC PC+ C+
Sbjct: 63  TLQFSNDDASPNSPQSFITSNRGEYLMNISIGTPPVPILAIADTGSDLIWTQCNPCEDCY 122

Query: 148 DQATPIFDPKQSSSFSKISCRSELCGALPTSTCSSD--GCEYLYTYGDSSSTQGVLAFET 207
            Q +P+FDPK+SS++ K+SC S  C AL  ++CS+D   C Y  TYGD+S T+G +A +T
Sbjct: 123 QQTSPLFDPKESSTYRKVSCSSSQCRALEDASCSTDENTCSYTITYGDNSYTKGDVAVDT 182

Query: 208 FTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGLGRGPLSLVSQLKEQ---KFAYC 267
            T G S    +S+  +  GCG++N G     G+G++GLG G  SLVSQL++    KF+YC
Sbjct: 183 VTMGSSGRRPVSLRNMIIGCGHENTGTFDPAGSGIIGLGGGSTSLVSQLRKSINGKFSYC 242

Query: 268 LTAI--DDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPSQPSFYYLSLQGISVGGTQL 327
           L     +    S +  G+   +    S D + +T ++K     ++Y+L+L+ ISVG  ++
Sbjct: 243 LVPFTSETGLTSKINFGTNGIV----SGDGVVSTSMVKK-DPATYYFLNLEAISVGSKKI 302

Query: 328 SIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFIAQMNLPVDDSGTGGLDLCF 387
               + F     G G ++IDSGTT+T + S  +  L++   + +         G L LC+
Sbjct: 303 QFTSTIF---GTGEGNIVIDSGTTLTLLPSNFYYELESVVASTIKAERVQDPDGILSLCY 362

Query: 388 NLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLCLAIGSSRGMSIFGNLQQQN 447
                ++  +VP +T HFKG D++L   N  +  S+  + C A  ++  ++IFGNL Q N
Sbjct: 363 R---DSSSFKVPDITVHFKGGDVKLGNLNTFVAVSE-DVSCFAFAANEQLTIFGNLAQMN 422

Query: 448 FMVVHDLQEETLSFLPTQCDSI 462
           F+V +D    T+SF  T C  +
Sbjct: 423 FLVGYDTVSGTVSFKKTDCSQM 431

[Close]

BLAST of Cmc08g0227131.1 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 256.1 bits (653), Expect = 5.2e-68
Identity = 138/368 (37.50%), Postives = 216/368 (58.70%), Query Frame = 0


Query: 98  DQVKAPVVA----GNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIF 157
           + +  PVV+    G+GE+  ++ +G+P +    ++DTGSD+ W QC+PC  C+ Q+ P+F
Sbjct: 145 EDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVF 204

Query: 158 DPKQSSSFSKISCRSELCGALPTSTCSSDGCEYLYTYGDSSSTQGVLAFETFTFGDSTED 217
           +P  SS++  ++C +  C  L TS C S+ C Y  +YGD S T G LA +T TFG+S + 
Sbjct: 205 NPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGK- 264

Query: 218 QISIPGLGFGCGNDNNGDGFSQGAGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSL 277
              I  +  GCG+DN G  F+  AGL+GLG G LS+ +Q+K   F+YCL   D  K SSL
Sbjct: 265 ---INNVALGCGHDNEG-LFTGAAGLLGLGGGVLSITNQMKATSFSYCLVDRDSGKSSSL 324

Query: 278 LLGSLANITPKTSKDEMKATPLIKNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGS 337
              S+     +    +  A PL++N    +FYY+ L G SVGG ++ +P + F++   GS
Sbjct: 325 DFNSV-----QLGGGDATA-PLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGS 384

Query: 338 GGVIIDSGTTITYIESTAFSSLKNEFI-AQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPK 397
           GGVI+D GT +T +++ A++SL++ F+   +NL    S     D C++  + +T V+VP 
Sbjct: 385 GGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLST-VKVPT 444

Query: 398 LTFHFKGA-DLELPGENYMIGDSKTGLLCLAIG-SSRGMSIFGNLQQQNFMVVHDLQEET 457
           + FHF G   L+LP +NY+I    +G  C A   +S  +SI GN+QQQ   + +DL +  
Sbjct: 445 VAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNV 500

Query: 458 LSFLPTQC 459
           +     +C
Sbjct: 505 IGLSGNKC 500

The following BLAST results are available for this feature:

BLAST of Cmc08g0227131.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008442220.1	3.5e-260	100.00	PREDICTED: aspartic proteinase nepenthesin-1 [Cucumis melo] >KAA0041170.1 aspart...	[more]
TYK02448.1	8.7e-259	99.57	aspartic proteinase nepenthesin-1 [Cucumis melo var. makuwa]	[more]
XP_011653928.1	2.1e-252	96.53	aspartic proteinase nepenthesin-1 [Cucumis sativus] >KGN54860.1 hypothetical pro...	[more]
XP_038883313.1	1.4e-227	89.08	aspartic proteinase nepenthesin-1 [Benincasa hispida]	[more]
XP_022928703.1	1.2e-210	83.48	aspartic proteinase nepenthesin-1 [Cucurbita moschata]	[more]

BLAST of Cmc08g0227131.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q766C3	2.0e-117	51.45	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	1.0e-113	49.18	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q7XV21	1.4e-70	34.89	Aspartyl protease 37 OS=Oryza sativa subsp. japonica OX=39947 GN=AP37 PE=3 SV=2	[more]
Q6XBF8	2.4e-70	36.26	Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1	[more]
Q9LS40	7.3e-67	37.50	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]

BLAST of Cmc08g0227131.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TD10	1.7e-260	100.00	Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A1S3B573	1.7e-260	100.00	aspartic proteinase nepenthesin-1 OS=Cucumis melo OX=3656 GN=LOC103486136 PE=3 S...	[more]
A0A5D3BTY9	4.2e-259	99.57	Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A0A0KYT9	1.0e-252	96.53	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G55468...	[more]
A0A6J1EPU6	5.6e-211	83.48	aspartic proteinase nepenthesin-1 OS=Cucurbita moschata OX=3662 GN=LOC111435534 ...	[more]

BLAST of Cmc08g0227131.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G03200.1	1.8e-153	61.22	Eukaryotic aspartyl protease family protein	[more]
AT1G25510.1	1.7e-71	38.55	Eukaryotic aspartyl protease family protein	[more]
AT5G33340.1	1.7e-71	36.26	Eukaryotic aspartyl protease family protein	[more]
AT1G64830.1	1.8e-68	35.07	Eukaryotic aspartyl protease family protein	[more]
AT3G18490.1	5.2e-68	37.50	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 430..445 score: 26.0 coord: 117..137 score: 43.19 coord: 336..347 score: 38.11
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 111..277 e-value: 3.9E-52 score: 177.0
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 281..461 e-value: 2.0E-53 score: 182.9
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 92..276 e-value: 1.4E-55 score: 190.3
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 105..459
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 304..453 e-value: 5.4E-36 score: 123.8
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 28..460
None	No IPR available	PANTHER	PTHR47967:SF23	OS08G0469000 PROTEIN	coord: 28..460
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 336..347
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 111..454 score: 42.918968
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 110..458 e-value: 5.19273E-111 score: 326.527

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cmc08g0227131	Cmc08g0227131	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cmc08g0227131.1-exon	Cmc08g0227131.1-exon-CMiso1.1chr08:19937946..19938356	exon
Cmc08g0227131.1-exon	Cmc08g0227131.1-exon-CMiso1.1chr08:19938497..19939015	exon
Cmc08g0227131.1-exon	Cmc08g0227131.1-exon-CMiso1.1chr08:19939221..19940019	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cmc08g0227131.1-three_prime_utr	Cmc08g0227131.1-three_prime_utr-CMiso1.1chr08:19937946..19938131	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cmc08g0227131.1-cds	Cmc08g0227131.1-cds-CMiso1.1chr08:19938132..19938356	CDS
Cmc08g0227131.1-cds	Cmc08g0227131.1-cds-CMiso1.1chr08:19938497..19939015	CDS
Cmc08g0227131.1-cds	Cmc08g0227131.1-cds-CMiso1.1chr08:19939221..19939862	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cmc08g0227131.1-five_prime_utr	Cmc08g0227131.1-five_prime_utr-CMiso1.1chr08:19939863..19940019	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cmc08g0227131.1	Cmc08g0227131.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016020	membrane
molecular_function	GO:0004190	aspartic-type endopeptidase activity