Spg036780 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg036780
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	kunitz trypsin inhibitor 2
Location	scaffold5: 46218159 .. 46220266 (+)
RNA-Seq Expression	Spg036780
Synteny	Spg036780

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAAGTTCGCATTACTGTCTTTTCTTTTCATCGTCATTGCCTCTGCTGAGGTACGCTTCTGCAAAGCCGGCGCCTCGCCGGACGCCGTCCTCGACGTCAACGGAGAGAAGCTCCGAGCCGGCGTCAACTACTCCATCCTGCCGTATTTTCGCAGAAACGCCGGCGGACTGACGATCGGCAGTATCCGAAAGGGACACGATCGATGTCCAGTACTCAACATCGTCCCAGGATTATACGAATCATACGACGGCCTTCCGGCGACATTTTCGCCCATAAACCCTAAGAAAGGCGTCGTTCGAGTTTCCACCGATCTGAACATCCAATTTCAGGCGAACGGCAAGTGCGGCAAATCGACGGTCTGGAAAGTAGCTAAATTGGATCAATCCACGGGACAGTATTTCGTGACGATCGGCGGAACCAAAGGAAACCCAGGGCGCGAGACGCTCGACAGTTGGTTCAAAATTGAGAAGTTGAGGCAAAATTATTACAAGTTGGTGTACTGTCCAACAAGTGTGTGTAAGTACTGCAAAGTTACATGCAAAGACGTCGGCATATTCTATGGGAATGGAAAGATGGCTCTTGTTTTGAACGATGCGCCATATCCCGTTATGTTCAAGAAATTATTAGGTTAAAGTTGAAGCTGTGAGAGAGGGAAGGGAAAGAAGAGGTTGGTTTTGGTTAAATGAATAATCACGAGTTCATGAAATGAATTGTTGTAATTATGTAACAGTGTGTTTATCTAAATAAAAAGATTTGGTTGTTTGTGGTTACCTGAATCTTAGGTAGGGTTTTTGGCAAAATATTAGAAATAATAACGTTAAATTACAAGTTTAGTTCGTCTATGAACTTGGTTGTATCAATTTGGTCGTTGAATTTTAAAAAGCGTCTAATAGGTACTTGAACTTCCAATTTTGTGTTTATTAGATCCCTTACTTTAAAAATGTGTCTAATAGGTCTCTAAACTTTCAATTTTGTGTCTAATAAGTTCCTAACATGCATATTCAAGATTATTTTTAAAAATTAACCAATCTATTAGATATAAAATTGAATTTTATGTACATTGGGACCCAAAACTTCTAATTTTGTATCTAGTCGGCTTGTGAATTTTAAAAATTGTTGAATAGGTCAAGGACTTATTAGACACACAAAATTAAAAGTTTAGAGACTTATTGGAAACAAAAGTGAAAGTATACGGACGATATACGGATACTTTTTAAAGTTCATCGACCTATTGGACACGAAATTGAAAGTTCGAAGACTAATTTGACACGACCCTGAAAGTTACCTAGAAATAACGTTCCAAGGTACAAAGTGGTGATGTACTCAAGTACTATAAAAGAAGTCTATGGTTTTGATTTTAAATCATCCAAAGCTTTTTTTTAAAAGAAAAAAAAAAAGAACATAAATATGATATACTAATTCTAGTGGTATAAAGCTTTGAGAGAGTGTGTTTACTTTTGTTATTGAGTTCAAGAAGATAAATTTGTATAATTTCTTATATATAATTGTTAGGTGTATGGTAAAAAAATATAGGGCAAATTGCAAAAACAACCCCTGAAGTATGATGTTTGTAACAATTACACCCTTAAAGTTTCAATTGTAAAAATTAAACCCTCCAACTTTCAAAAGTGTTAAAATTAGAACCTTAAACTTTTGAATCTTAAAATTAAACCCCCAATCTATGATAAATGTAAAAATTGAGTCCATCTCATATTTTTCCTTCTCTCTCCTCTTTCCTCTCTCTCCTTTCTCCTTCTACCTCTTCATCATTTCCATCACCCTCTCTCTCTCTCAGGAATAATAATGGAATCAGTGATTAATTGGCTTTCTTTGTTCGTTTTTGGCATTTAAATTTCCTTCTCTTTCTTTGATCTTAACCCTTCTTTGAGCTTCTTCCATTAGCCTTTTGTACAATTTCTCCTCTGCATCTCTGAGAAATTTGAACTTGGGAGGAGGAGAAGATCTCAACCTGTTCAAATTTTGCTCCAATTATGCCAACGATTCAAAGAGGGGATTGAATCCATGGAGCTTGTACGAGTCTAAAGAGCTCAGAGGAGAAGCCTTCAATGGCGAAGAAGCCATTGGAGTGAAGAACGACGTTTGA

mRNA sequence

ATGAAGAAGTTCGCATTACTGTCTTTTCTTTTCATCGTCATTGCCTCTGCTGAGGTACGCTTCTGCAAAGCCGGCGCCTCGCCGGACGCCGTCCTCGACGTCAACGGAGAGAAGCTCCGAGCCGGCGTCAACTACTCCATCCTGCCGTATTTTCGCAGAAACGCCGGCGGACTGACGATCGGCAGTATCCGAAAGGGACACGATCGATGTCCAGTACTCAACATCGTCCCAGGATTATACGAATCATACGACGGCCTTCCGGCGACATTTTCGCCCATAAACCCTAAGAAAGGCGTCGTTCGAGTTTCCACCGATCTGAACATCCAATTTCAGGCGAACGGCAAGTGCGGCAAATCGACGGTCTGGAAAGTAGCTAAATTGGATCAATCCACGGGACAGTATTTCGTGACGATCGGCGGAACCAAAGGAAACCCAGGGCGCGAGACGCTCGACAGTTGGTTCAAAATTGAGAAGTTGAGGCAAAATTATTACAAGTTGGTGTACTGTCCAACAAGTGTGTGTAAGTACTGCAAAGTTACATGCAAAGACGTCGGCATATTCTATGGGAATGGAAAGATGGCTCTTGTTTTGAACGATGCGCCATATCCCGTTATCCTTTTGTACAATTTCTCCTCTGCATCTCTGAGAAATTTGAACTTGGGAGGAGGAGAAGATCTCAACCTGTTCAAATTTTGCTCCAATTATGCCAACGATTCAAAGAGGGGATTGAATCCATGGAGCTTGTACGAGTCTAAAGAGCTCAGAGGAGAAGCCTTCAATGGCGAAGAAGCCATTGGAGTGAAGAACGACGTTTGA

Coding sequence (CDS)

ATGAAGAAGTTCGCATTACTGTCTTTTCTTTTCATCGTCATTGCCTCTGCTGAGGTACGCTTCTGCAAAGCCGGCGCCTCGCCGGACGCCGTCCTCGACGTCAACGGAGAGAAGCTCCGAGCCGGCGTCAACTACTCCATCCTGCCGTATTTTCGCAGAAACGCCGGCGGACTGACGATCGGCAGTATCCGAAAGGGACACGATCGATGTCCAGTACTCAACATCGTCCCAGGATTATACGAATCATACGACGGCCTTCCGGCGACATTTTCGCCCATAAACCCTAAGAAAGGCGTCGTTCGAGTTTCCACCGATCTGAACATCCAATTTCAGGCGAACGGCAAGTGCGGCAAATCGACGGTCTGGAAAGTAGCTAAATTGGATCAATCCACGGGACAGTATTTCGTGACGATCGGCGGAACCAAAGGAAACCCAGGGCGCGAGACGCTCGACAGTTGGTTCAAAATTGAGAAGTTGAGGCAAAATTATTACAAGTTGGTGTACTGTCCAACAAGTGTGTGTAAGTACTGCAAAGTTACATGCAAAGACGTCGGCATATTCTATGGGAATGGAAAGATGGCTCTTGTTTTGAACGATGCGCCATATCCCGTTATCCTTTTGTACAATTTCTCCTCTGCATCTCTGAGAAATTTGAACTTGGGAGGAGGAGAAGATCTCAACCTGTTCAAATTTTGCTCCAATTATGCCAACGATTCAAAGAGGGGATTGAATCCATGGAGCTTGTACGAGTCTAAAGAGCTCAGAGGAGAAGCCTTCAATGGCGAAGAAGCCATTGGAGTGAAGAACGACGTTTGA

Protein sequence

MKKFALLSFLFIVIASAEVRFCKAGASPDAVLDVNGEKLRAGVNYSILPYFRRNAGGLTIGSIRKGHDRCPVLNIVPGLYESYDGLPATFSPINPKKGVVRVSTDLNIQFQANGKCGKSTVWKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLRQNYYKLVYCPTSVCKYCKVTCKDVGIFYGNGKMALVLNDAPYPVILLYNFSSASLRNLNLGGGEDLNLFKFCSNYANDSKRGLNPWSLYESKELRGEAFNGEEAIGVKNDV

Homology

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BLAST of Spg036780 vs. NCBI nr
Match: XP_008437082.1 (PREDICTED: miraculin [Cucumis melo] >TYJ99076.1 miraculin [Cucumis melo var. makuwa])

HSP 1 Score: 290.8 bits (743), Expect = 1.2e-74
Identity = 141/205 (68.78%), Postives = 168/205 (81.95%), Query Frame = 0


Query: 1   MKKFALLSFLFIVIASAEVRFCKAGASPDAVLDVNGEKLRAGVNYSILPYFRRNAGGLTI 60
           MK FALL FLFIVIAS+EVRFC+A ASPDAVLD +G+KLRAG  Y IL  + RN+GGL+I
Sbjct: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60

Query: 61  GSIRKGHDRCPVLNIVPGLYESYDGLPATFSPINPKKGVVRVSTDLNIQFQANGKCGKST 120
           G I  G+++CP+ NI+P  Y+  DGLPATFSP+NPKKGVVRVSTDLNI+F+A+ +CG ST
Sbjct: 61  GGI-YGYEKCPI-NILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGIST 120

Query: 121 VWKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLRQNYYKLVYCPTSVCKYCKVT 180
           VWKV K DQ   QYFVT+GGTKGNPGRET+ +WFK+EK  +N YK VYCPT VCKYCKV 
Sbjct: 121 VWKVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKN-YKFVYCPT-VCKYCKVM 180

Query: 181 CKDVGIFYGNGKMALVLNDAPYPVI 206
           CKDVG+FY NG+    LNDAP+PV+
Sbjct: 181 CKDVGLFYKNGRRIFALNDAPFPVM 201

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BLAST of Spg036780 vs. NCBI nr
Match: XP_038895396.1 (miraculin-like [Benincasa hispida] >XP_038895399.1 miraculin-like [Benincasa hispida])

HSP 1 Score: 290.4 bits (742), Expect = 1.6e-74
Identity = 141/205 (68.78%), Postives = 166/205 (80.98%), Query Frame = 0


Query: 1   MKKFALLSFLFIVIASAEVRFCKAGASPDAVLDVNGEKLRAGVNYSILPYFRRNAGGLTI 60
           MK FALLSFLFIVI S+EVRFC+A  SPDAVLD +G+KLR+   Y IL  + RN+GGL+I
Sbjct: 1   MKNFALLSFLFIVIVSSEVRFCRADVSPDAVLDTDGKKLRSSDQYYILSVYSRNSGGLSI 60

Query: 61  GSIRKGHDRCPVLNIVPGLYESYDGLPATFSPINPKKGVVRVSTDLNIQFQANGKCGKST 120
           GSI++G ++CP+ NI+P  YE   GL ATF PINPKKGVVRVSTDLNIQF+A+ +CG+ST
Sbjct: 61  GSIQQGKEKCPI-NILPESYEYLHGLAATFFPINPKKGVVRVSTDLNIQFEASTRCGEST 120

Query: 121 VWKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLRQNYYKLVYCPTSVCKYCKVT 180
           VWK+ K DQ   QYFVTIGG KGNPGRET+ +WFKIEK   N YKLV+CPT VCKYCKV 
Sbjct: 121 VWKIGKFDQYLKQYFVTIGGVKGNPGRETVGNWFKIEKYGNN-YKLVHCPT-VCKYCKVI 180

Query: 181 CKDVGIFYGNGKMALVLNDAPYPVI 206
           CKDVGIFY NGK  L LND+P+PV+
Sbjct: 181 CKDVGIFYKNGKRVLALNDSPFPVM 202

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BLAST of Spg036780 vs. NCBI nr
Match: XP_022137472.1 (kunitz trypsin inhibitor 2 [Momordica charantia])

HSP 1 Score: 273.1 bits (697), Expect = 2.6e-69
Identity = 133/205 (64.88%), Postives = 164/205 (80.00%), Query Frame = 0


Query: 1   MKKFALLSFLFIVIASAEVRFCKAGASPDAVLDVNGEKLRAGVNYSILPYFRRNAGGLTI 60
           MK FALL FLF +IAS EVR CK+ ASPDAVLD++G+KLRAGVNY ILP  R   GGLT+
Sbjct: 1   MKNFALLCFLFALIASTEVRICKSDASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLTL 60

Query: 61  GSIRKGHDRCPVLNIVPGLYESYDGLPATFSPINPKKGVVRVSTDLNIQFQANGKCGKST 120
           G+I  GHD+CP L++V   +E  +GLPATF+P+NPKKGVVRVSTDLN++F+A+  C KST
Sbjct: 61  GNI--GHDKCP-LHVVQEQFEISNGLPATFAPVNPKKGVVRVSTDLNVEFEASTVCAKST 120

Query: 121 VWKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLRQNYYKLVYCPTSVCKYCKVT 180
           VWK+ K D+ST Q+ V IGG +GNPG+ETLD+WFKIEK  ++ YK V+CP SVCK+CKV 
Sbjct: 121 VWKLDKFDESTRQWLVAIGGIRGNPGQETLDNWFKIEKHGKD-YKFVFCP-SVCKFCKVI 180

Query: 181 CKDVGIFYGNGKMALVLNDAPYPVI 206
           CKDVGIF  NG  AL L+D P+PV+
Sbjct: 181 CKDVGIFIKNGNRALALSDTPFPVM 200

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BLAST of Spg036780 vs. NCBI nr
Match: XP_008437071.1 (PREDICTED: miraculin-like [Cucumis melo] >TYJ99074.1 miraculin-like [Cucumis melo var. makuwa])

HSP 1 Score: 271.2 bits (692), Expect = 9.9e-69
Identity = 138/208 (66.35%), Postives = 157/208 (75.48%), Query Frame = 0


Query: 1   MKKFALLSFLF--IVIASAEVRFCKAGASPDAVLDVNGEKLRAGVNYSILPYFR-RNAGG 60
           MKKFALLSFLF  IVIAS+E+RFC+A ASPDAVLD +G+KLR    Y ILP F     GG
Sbjct: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60

Query: 61  LTIGSIRKGHDRCPVLNIVPGLYESYDGLPATFSPINPKKGVVRVSTDLNIQFQANGKCG 120
           L IG+IRK +DRC + N+V   YE  DG P TF PINPKKGVVRVSTDLNI+F A  +C 
Sbjct: 61  LAIGNIRKEYDRCGI-NVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCR 120

Query: 121 KSTVWKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLRQNYYKLVYCPTSVCKYC 180
           KSTVWK+   D+   QYFVTIGGTKGNPGRET ++WFKIEK  +  YKLVYCP  VCKYC
Sbjct: 121 KSTVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCP-RVCKYC 180

Query: 181 KVTCKDVGIFYGNGKMALVLNDAPYPVI 206
           KV CKD+GIF  NG   LVL+D P+PVI
Sbjct: 181 KVMCKDIGIFENNGIRGLVLSDTPFPVI 206

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BLAST of Spg036780 vs. NCBI nr
Match: XP_038893656.1 (miraculin-like [Benincasa hispida])

HSP 1 Score: 269.2 bits (687), Expect = 3.8e-68
Identity = 131/209 (62.68%), Postives = 163/209 (77.99%), Query Frame = 0


Query: 1   MKKFALLSFLFIVIASAEVR--FCKAGASPDAVLDVNGEKLRAGVNYSILPYFRRNAGGL 60
           MK F LLS +FIVI ++  R  FC+  ASP+A+LD NG+KLR G +Y ILP+F RN GGL
Sbjct: 1   MKSFELLSLIFIVIVASTERQQFCRVNASPEAILDTNGKKLRGGDHYYILPFFSRNTGGL 60

Query: 61  TIGSIRKGH-DRCPVLNIVPGLYESYDGLPATFSPINPKKGVVRVSTDLNIQFQANGKCG 120
            +G+I++ + + CP LN+VP  YE ++GLP TFSP+NPKKGV+RVSTDLNIQF+ N  C 
Sbjct: 61  ALGNIQREYSNECP-LNVVPEPYEYFNGLPTTFSPVNPKKGVIRVSTDLNIQFERNTMCA 120

Query: 121 KSTVWKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKL-RQNYYKLVYCPTSVCKY 180
           KSTVWK++K +Q   QY VTIGGTKG+PG ET+++WFKIEK    N YK VYCPT VCKY
Sbjct: 121 KSTVWKISKFEQ---QYLVTIGGTKGDPGPETMENWFKIEKYNNNNIYKFVYCPT-VCKY 180

Query: 181 CKVTCKDVGIFYGNGKMALVLNDAPYPVI 206
           CKV CKDVGIF  NG+MAL L+DAP+PV+
Sbjct: 181 CKVMCKDVGIFVKNGRMALALSDAPFPVM 204

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BLAST of Spg036780 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 180.3 bits (456), Expect = 3.0e-44
Identity = 103/212 (48.58%), Postives = 130/212 (61.32%), Query Frame = 0


Query: 1   MKKFALLSFLF-----IVIASAEVRFCKAGASPDAVLDVNGEKLRAGVNYSILPYFRRNA 60
           MK+  +LS  F     ++ A+A      A ++P+ VLD++GEKLR G NY I+P  R + 
Sbjct: 1   MKELTMLSLSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHG 60

Query: 61  GGLTIGSIR-KGHDRCPVLNIVPGLYESYDGLPATFSPINPKKGVVRVSTDLNIQFQANG 120
           GGLT+ +    G   CP   +V    E     P  F P NPK+ VVRVSTDLNI F A  
Sbjct: 61  GGLTVSATTPNGTFVCPP-RVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFM 120

Query: 121 KC--GKSTVWKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKL-RQNYYKLVYCPT 180
            C    STVW++ K D+STGQYFVTIGG KGNPG ET+ SWFKIE+     +YKLV+CPT
Sbjct: 121 PCRWTSSTVWRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPT 180

Query: 181 SVCKYCKVTCKDVGIFYG-NGKMALVLNDAPY 203
            VC  CKV C DVGI+    G+  L L+D P+
Sbjct: 181 -VCGSCKVKCGDVGIYIDQKGRRRLALSDKPF 210

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BLAST of Spg036780 vs. ExPASy Swiss-Prot
Match: Q9LMU2 (Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 7.9e-37
Identity = 79/200 (39.50%), Postives = 127/200 (63.50%), Query Frame = 0


Query: 7   LSFLFIVIA-SAEVRFCKAGASPDAVLDVNGEKLRAGVNYSILPYFRRNAGGLTIGSIRK 66
           L ++F+++A     R     A+ + V D+NG+ L  GVNY ILP  R   GGLT+ +++ 
Sbjct: 4   LLYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKT 63

Query: 67  GHDRCPVLNIVPGLYESYDGLPATFSPINPKKGVVRVSTDLNIQFQANGKCGKSTVWKVA 126
             + CP  +++   +E   GLP  FSP + K   + VSTD+NI+F        +++W++A
Sbjct: 64  --ETCPT-SVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKF------SPTSIWELA 123

Query: 127 KLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLRQNYYKLVYCPTSVCKYCKVTCKDVG 186
             D++T Q+F++  G +GNPG++T+D+WFKI+K  ++ YK+ +CPT VC +CKV C+DVG
Sbjct: 124 NFDETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKD-YKIRFCPT-VCNFCKVICRDVG 183

Query: 187 IFYGNGKMALVLNDAPYPVI 206
           +F  +GK  L L+D P  V+
Sbjct: 184 VFVQDGKRRLALSDVPLKVM 191

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BLAST of Spg036780 vs. ExPASy Swiss-Prot
Match: P32765 (21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 2.6e-27
Identity = 78/203 (38.42%), Postives = 105/203 (51.72%), Query Frame = 0


Query: 1   MKKFALLSFLFIVIASAEVRFCKAGASPDAVLDVNGEKLRAGVNYSILPYFR-RNAGGLT 60
           MK    +  L     S    F  A A+   VLD +G++L+ GV Y +L        GGL 
Sbjct: 1   MKTATAVVLLLFAFTSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLA 60

Query: 61  IGSIRKGHDRCPVLNIVPGLYESYDGLPATFSPINPKKGVVRVSTDLNIQFQ--ANGKCG 120
           +G  R     CP + +V    +  +G P  FS  + K  VVRVSTD+NI+F    +  C 
Sbjct: 61  LG--RATGQSCPEI-VVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCS 120

Query: 121 KSTVWKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLRQNYYKLVYCPTSVCKYC 180
            STVW++   D S G+++VT  G KG PG  TL SWFKIEK     YK  +CP SVC  C
Sbjct: 121 TSTVWRLDNYDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCP-SVCDSC 180

Query: 181 KVTCKDVGIFY-GNGKMALVLND 200
              C D+G     +G++ L L+D
Sbjct: 181 TTLCSDIGRHSDDDGQIRLALSD 199

[Close]

BLAST of Spg036780 vs. ExPASy Swiss-Prot
Match: Q8RXD5 (Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 1.0e-20
Identity = 65/178 (36.52%), Postives = 92/178 (51.69%), Query Frame = 0


Query: 30  AVLDVNGEKLRAGVNYSILPYFRRNAGGLTIGSIRKGHDRCPVLNIVPGLYESYDGLPAT 89
           AV+D++G  +    +Y +LP  R   GGLT+    +G   CP  +IV    E  +G+P  
Sbjct: 29  AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAG--RGGQPCP-YDIVQESSEVDEGIPVK 88

Query: 90  FSPINPKKGVVRVSTDLNIQFQANGK-CGKSTVWKVAKLDQSTGQYFVTIGGTKGNPGRE 149
           FS    K   V  S +LNI+       C +ST W+V + D    QYFV  G      G++
Sbjct: 89  FSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQD 148

Query: 150 TLDSWFKIEKLRQNYYKLVYCPTSVCKYCKVTCKDVGIFYGN-GKMALVLNDAPYPVI 206
           +L S+FKIEK  ++ YK V+CP + C      C DVGIF    G   L L+D P+ V+
Sbjct: 149 SLKSFFKIEKSGEDAYKFVFCPRT-CDSGNPKCSDVGIFIDELGVRRLALSDKPFLVM 201

[Close]

BLAST of Spg036780 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 4.1e-17
Identity = 54/178 (30.34%), Postives = 88/178 (49.44%), Query Frame = 0


Query: 31  VLDVNGEKLRAGVNYSILPYFRRNAGGLTIGSIRKGHDRCPVLNIVPGLYESYDGLPATF 90
           V+D +GE +     Y I P    N GG T+ +   G+  CP L++     E   G+   F
Sbjct: 27  VIDTSGEPVEDDEEYFIRPAITGNGGGSTLVT---GNGPCP-LHVGLDNTEGTLGVAVKF 86

Query: 91  SPINPK--KGVVRVSTDLNIQFQANGKCGKSTVWKVAKLDQSTGQYFVTIGGTKGNPGRE 150
           +P  P+     VR++ DL + F  +  CG+ST W++ + D ++G+  +  G   G   + 
Sbjct: 87  TPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDWRLGEKDATSGRRLIVTGRDNGAGSQ- 146

Query: 151 TLDSWFKIEKLRQ-NYYKLVYCPTSVCKYCKVTCKDVGIFYGNGKMALVLNDAPYPVI 206
              ++F+I + +    Y + +CPT  C  CKV C  VG+   NGK  L L+    PV+
Sbjct: 147 --GNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTVGVIRENGKNLLALDGDALPVV 197

[Close]

BLAST of Spg036780 vs. ExPASy TrEMBL
Match: A0A0A0LT81 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043220 PE=3 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 2.6e-75
Identity = 141/205 (68.78%), Postives = 169/205 (82.44%), Query Frame = 0


Query: 1   MKKFALLSFLFIVIASAEVRFCKAGASPDAVLDVNGEKLRAGVNYSILPYFRRNAGGLTI 60
           M+ FALL FLFIVIAS+EVRFC+A ASPDAVLD +G+KLRAG  Y IL  + RN+GGL+I
Sbjct: 1   MRNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60

Query: 61  GSIRKGHDRCPVLNIVPGLYESYDGLPATFSPINPKKGVVRVSTDLNIQFQANGKCGKST 120
           G I  G+++CP+ NI+P  Y+   GLPATFSPINPKKGVVRVSTDLNIQF+AN +CG ST
Sbjct: 61  GGI-YGYEKCPI-NILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGIST 120

Query: 121 VWKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLRQNYYKLVYCPTSVCKYCKVT 180
           VWKV K D+   QYFVT+GG KGNPGRET+++WFK+EK  +N YKLVYCPT VCKYCKV 
Sbjct: 121 VWKVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKN-YKLVYCPT-VCKYCKVV 180

Query: 181 CKDVGIFYGNGKMALVLNDAPYPVI 206
           CKDVG+FY NG+  + LNDAP+PV+
Sbjct: 181 CKDVGLFYKNGRRVIALNDAPFPVM 201

[Close]

BLAST of Spg036780 vs. ExPASy TrEMBL
Match: A0A5D3BJ02 (Miraculin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002800 PE=3 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 5.8e-75
Identity = 141/205 (68.78%), Postives = 168/205 (81.95%), Query Frame = 0


Query: 1   MKKFALLSFLFIVIASAEVRFCKAGASPDAVLDVNGEKLRAGVNYSILPYFRRNAGGLTI 60
           MK FALL FLFIVIAS+EVRFC+A ASPDAVLD +G+KLRAG  Y IL  + RN+GGL+I
Sbjct: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60

Query: 61  GSIRKGHDRCPVLNIVPGLYESYDGLPATFSPINPKKGVVRVSTDLNIQFQANGKCGKST 120
           G I  G+++CP+ NI+P  Y+  DGLPATFSP+NPKKGVVRVSTDLNI+F+A+ +CG ST
Sbjct: 61  GGI-YGYEKCPI-NILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGIST 120

Query: 121 VWKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLRQNYYKLVYCPTSVCKYCKVT 180
           VWKV K DQ   QYFVT+GGTKGNPGRET+ +WFK+EK  +N YK VYCPT VCKYCKV 
Sbjct: 121 VWKVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKN-YKFVYCPT-VCKYCKVM 180

Query: 181 CKDVGIFYGNGKMALVLNDAPYPVI 206
           CKDVG+FY NG+    LNDAP+PV+
Sbjct: 181 CKDVGLFYKNGRRIFALNDAPFPVM 201

[Close]

BLAST of Spg036780 vs. ExPASy TrEMBL
Match: A0A1S3AST3 (miraculin OS=Cucumis melo OX=3656 GN=LOC103482610 PE=3 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 5.8e-75
Identity = 141/205 (68.78%), Postives = 168/205 (81.95%), Query Frame = 0


Query: 1   MKKFALLSFLFIVIASAEVRFCKAGASPDAVLDVNGEKLRAGVNYSILPYFRRNAGGLTI 60
           MK FALL FLFIVIAS+EVRFC+A ASPDAVLD +G+KLRAG  Y IL  + RN+GGL+I
Sbjct: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60

Query: 61  GSIRKGHDRCPVLNIVPGLYESYDGLPATFSPINPKKGVVRVSTDLNIQFQANGKCGKST 120
           G I  G+++CP+ NI+P  Y+  DGLPATFSP+NPKKGVVRVSTDLNI+F+A+ +CG ST
Sbjct: 61  GGI-YGYEKCPI-NILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGIST 120

Query: 121 VWKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLRQNYYKLVYCPTSVCKYCKVT 180
           VWKV K DQ   QYFVT+GGTKGNPGRET+ +WFK+EK  +N YK VYCPT VCKYCKV 
Sbjct: 121 VWKVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKN-YKFVYCPT-VCKYCKVM 180

Query: 181 CKDVGIFYGNGKMALVLNDAPYPVI 206
           CKDVG+FY NG+    LNDAP+PV+
Sbjct: 181 CKDVGLFYKNGRRIFALNDAPFPVM 201

[Close]

BLAST of Spg036780 vs. ExPASy TrEMBL
Match: A0A6J1C8C4 (kunitz trypsin inhibitor 2 OS=Momordica charantia OX=3673 GN=LOC111008908 PE=3 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 1.3e-69
Identity = 133/205 (64.88%), Postives = 164/205 (80.00%), Query Frame = 0


Query: 1   MKKFALLSFLFIVIASAEVRFCKAGASPDAVLDVNGEKLRAGVNYSILPYFRRNAGGLTI 60
           MK FALL FLF +IAS EVR CK+ ASPDAVLD++G+KLRAGVNY ILP  R   GGLT+
Sbjct: 1   MKNFALLCFLFALIASTEVRICKSDASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLTL 60

Query: 61  GSIRKGHDRCPVLNIVPGLYESYDGLPATFSPINPKKGVVRVSTDLNIQFQANGKCGKST 120
           G+I  GHD+CP L++V   +E  +GLPATF+P+NPKKGVVRVSTDLN++F+A+  C KST
Sbjct: 61  GNI--GHDKCP-LHVVQEQFEISNGLPATFAPVNPKKGVVRVSTDLNVEFEASTVCAKST 120

Query: 121 VWKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLRQNYYKLVYCPTSVCKYCKVT 180
           VWK+ K D+ST Q+ V IGG +GNPG+ETLD+WFKIEK  ++ YK V+CP SVCK+CKV 
Sbjct: 121 VWKLDKFDESTRQWLVAIGGIRGNPGQETLDNWFKIEKHGKD-YKFVFCP-SVCKFCKVI 180

Query: 181 CKDVGIFYGNGKMALVLNDAPYPVI 206
           CKDVGIF  NG  AL L+D P+PV+
Sbjct: 181 CKDVGIFIKNGNRALALSDTPFPVM 200

[Close]

BLAST of Spg036780 vs. ExPASy TrEMBL
Match: A0A5D3BJ25 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002780 PE=3 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 4.8e-69
Identity = 138/208 (66.35%), Postives = 157/208 (75.48%), Query Frame = 0


Query: 1   MKKFALLSFLF--IVIASAEVRFCKAGASPDAVLDVNGEKLRAGVNYSILPYFR-RNAGG 60
           MKKFALLSFLF  IVIAS+E+RFC+A ASPDAVLD +G+KLR    Y ILP F     GG
Sbjct: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60

Query: 61  LTIGSIRKGHDRCPVLNIVPGLYESYDGLPATFSPINPKKGVVRVSTDLNIQFQANGKCG 120
           L IG+IRK +DRC + N+V   YE  DG P TF PINPKKGVVRVSTDLNI+F A  +C 
Sbjct: 61  LAIGNIRKEYDRCGI-NVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCR 120

Query: 121 KSTVWKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLRQNYYKLVYCPTSVCKYC 180
           KSTVWK+   D+   QYFVTIGGTKGNPGRET ++WFKIEK  +  YKLVYCP  VCKYC
Sbjct: 121 KSTVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCP-RVCKYC 180

Query: 181 KVTCKDVGIFYGNGKMALVLNDAPYPVI 206
           KV CKD+GIF  NG   LVL+D P+PVI
Sbjct: 181 KVMCKDIGIFENNGIRGLVLSDTPFPVI 206

[Close]

BLAST of Spg036780 vs. TAIR 10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 155.6 bits (392), Expect = 5.6e-38
Identity = 79/200 (39.50%), Postives = 127/200 (63.50%), Query Frame = 0


Query: 7   LSFLFIVIA-SAEVRFCKAGASPDAVLDVNGEKLRAGVNYSILPYFRRNAGGLTIGSIRK 66
           L ++F+++A     R     A+ + V D+NG+ L  GVNY ILP  R   GGLT+ +++ 
Sbjct: 4   LLYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKT 63

Query: 67  GHDRCPVLNIVPGLYESYDGLPATFSPINPKKGVVRVSTDLNIQFQANGKCGKSTVWKVA 126
             + CP  +++   +E   GLP  FSP + K   + VSTD+NI+F        +++W++A
Sbjct: 64  --ETCPT-SVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKF------SPTSIWELA 123

Query: 127 KLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLRQNYYKLVYCPTSVCKYCKVTCKDVG 186
             D++T Q+F++  G +GNPG++T+D+WFKI+K  ++ YK+ +CPT VC +CKV C+DVG
Sbjct: 124 NFDETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKD-YKIRFCPT-VCNFCKVICRDVG 183

Query: 187 IFYGNGKMALVLNDAPYPVI 206
           +F  +GK  L L+D P  V+
Sbjct: 184 VFVQDGKRRLALSDVPLKVM 191

[Close]

BLAST of Spg036780 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 102.1 bits (253), Expect = 7.4e-22
Identity = 65/178 (36.52%), Postives = 92/178 (51.69%), Query Frame = 0


Query: 30  AVLDVNGEKLRAGVNYSILPYFRRNAGGLTIGSIRKGHDRCPVLNIVPGLYESYDGLPAT 89
           AV+D++G  +    +Y +LP  R   GGLT+    +G   CP  +IV    E  +G+P  
Sbjct: 29  AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAG--RGGQPCP-YDIVQESSEVDEGIPVK 88

Query: 90  FSPINPKKGVVRVSTDLNIQFQANGK-CGKSTVWKVAKLDQSTGQYFVTIGGTKGNPGRE 149
           FS    K   V  S +LNI+       C +ST W+V + D    QYFV  G      G++
Sbjct: 89  FSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQD 148

Query: 150 TLDSWFKIEKLRQNYYKLVYCPTSVCKYCKVTCKDVGIFYGN-GKMALVLNDAPYPVI 206
           +L S+FKIEK  ++ YK V+CP + C      C DVGIF    G   L L+D P+ V+
Sbjct: 149 SLKSFFKIEKSGEDAYKFVFCPRT-CDSGNPKCSDVGIFIDELGVRRLALSDKPFLVM 201

The following BLAST results are available for this feature:

BLAST of Spg036780 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008437082.1	1.2e-74	68.78	PREDICTED: miraculin [Cucumis melo] >TYJ99076.1 miraculin [Cucumis melo var. mak...	[more]
XP_038895396.1	1.6e-74	68.78	miraculin-like [Benincasa hispida] >XP_038895399.1 miraculin-like [Benincasa his...	[more]
XP_022137472.1	2.6e-69	64.88	kunitz trypsin inhibitor 2 [Momordica charantia]	[more]
XP_008437071.1	9.9e-69	66.35	PREDICTED: miraculin-like [Cucumis melo] >TYJ99074.1 miraculin-like [Cucumis mel...	[more]
XP_038893656.1	3.8e-68	62.68	miraculin-like [Benincasa hispida]	[more]

BLAST of Spg036780 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P13087	3.0e-44	48.58	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
Q9LMU2	7.9e-37	39.50	Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1	[more]
P32765	2.6e-27	38.42	21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1	[more]
Q8RXD5	1.0e-20	36.52	Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1	[more]
A0A072TH68	4.1e-17	30.34	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]

BLAST of Spg036780 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LT81	2.6e-75	68.78	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043220 PE=3 SV=1	[more]
A0A5D3BJ02	5.8e-75	68.78	Miraculin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002800 PE=...	[more]
A0A1S3AST3	5.8e-75	68.78	miraculin OS=Cucumis melo OX=3656 GN=LOC103482610 PE=3 SV=1	[more]
A0A6J1C8C4	1.3e-69	64.88	kunitz trypsin inhibitor 2 OS=Momordica charantia OX=3673 GN=LOC111008908 PE=3 S...	[more]
A0A5D3BJ25	4.8e-69	66.35	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00278...	[more]

BLAST of Spg036780 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17860.1	5.6e-38	39.50	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73260.1	7.4e-22	36.52	kunitz trypsin inhibitor 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 30..59 score: 39.08 coord: 153..172 score: 29.41
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 30..211 e-value: 4.0E-48 score: 175.9
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 31..204 e-value: 1.2E-46 score: 158.8
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 8..205
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 31..47
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 30..206 e-value: 3.72871E-50 score: 160.971
None	No IPR available	GENE3D	2.80.10.50		coord: 28..208 e-value: 1.4E-58 score: 199.6
None	No IPR available	PANTHER	PTHR33107:SF5	KUNITZ TRYPSIN INHIBITOR 2	coord: 8..205
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 28..207

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg036780.1	Spg036780.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity