Spg012884 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg012884
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionabscisic acid 8'-hydroxylase 1-like
Locationscaffold1: 8398741 .. 8402071 (-)
RNA-Seq ExpressionSpg012884
SyntenySpg012884
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATATAATGTGTCCTTTTTCTGGTATGAATCTTTCCATTCTCATGCCTAAGCATCCAAGGATATACATTGGCCACCATTTAAATATATACCAAATCTTATCTTGTTGAAGACTTTGGTCATTCCTCCCTATATTTATATATTGAAGCATATTGGAAGAGTAATCCCACCACATTGGAACCATTCATTGTCTTCATCAATAGTCAATAGAGTTTGTTGGTGAAAATTGAAGATGCTGTGGAAGGAAGAACTTGTGTTCTTCGCTCAAAATTATTTTGACACTGCAGTGGCGGCAGTTCTTTCCATAGCTATTCTAGTGTTAACTTCGAAAGTTTGCTGGAGGTTGAGGAGCAAGAATGTTGGAGGAATTCCTGGTCACTTGGGGTTACCTTTTGTAGGGGAAACCTTCTCTTTTCTTTCTGCCAATAACAGTACCAAAGGGTGTTACCACTTTGTCAGGCTCCGCCGGTTATGGTTAGTGGTTCCTTCCCCCATGGTGATTATGGATATTCTTTACATAAGTACTATTGTCAGTATGACTTCTAATATTCCTTAAGTATGATTCCAATTCTTCCTCATTCGAGATCAAGGATCATTTTTCTGAATTTTTTGACTTTTAAACGTTTGGCTACAAAACTAGACTTTGCCTATCATCGTATATGAGTCAACTCCATTGAATTTTTACACCTTGACTACTGATATGAGTTGCATGTATTTGGGAAGGTACATGAATACACATGCATACATACATGTATACATACGTACATACATATAAACAAACATTACAATATACCTCCCGGACAGTTTAGAACATGGAACTGATGACTGTGATGAAACTAACCAGGCATGGTAGGTGGTTCAAGACAAGGCTATTTGGAAAGGTCCACGTGTTTGTTCCAAGTACAGAAGGGGCAAAAGCAATATTTGCCAATGATTTTGTCCAATTCAACAAGGGGTATGTGAAATCCATGGCAGATGCTGTTGGAGAGAAGAGTTTACTTTGCGTTTCCCATGAGAACCACAAGAGGATTAGGCGTCTTCTAGCTGATCCTTTCTCCATGACTTCTTTGTCCAAGTTTGTTAAAGAATTTGACGACATGCTCTCCAAAAGGCTAAAGAAACTAGTAAAAGATGGGAAAAGTTTTGTACTTCTTGATTTTAGCATGAAGGTTATAGAAAATAAAAATATAGTCATCTTTCTCAAACTTTTCTGTTTGACATGGGTGAGTGACCAACATATCTTATCTTGTGTGATTTGCAGATAACATTCGATGCCATGTGCAATATGCTAATGAGTCTAAGAGATGAATCTACGCTCAGACAGATTGAAAAAGACTGTATAGCAGTCTCTGATGCCATGTTATCTTTCCCATTCATGATTCCAGGAACTAGGTACTACAAAGGCATCAAGGTACAGTCAGCAACTTCAAGATATAGGCACCCAACTTAAGTGTATAATACAATTTAACGAGGTTTCATTCTACTTTAGTAACATATATACTAATTACCAATAAGTTGAGCAGGCACGAAACCGACTCATGGAAAGGTTTAGAGAGATAATTTCCAGGCGGCGGAGGGGAGAGGAGTCACCAGAAGACTTTCTGCAATCAATGTTGCAGAAAGATTCATATCCTCCTGATGAAAAGCTGGATGACTCAGAGATAATGGATAACCTTTTAACACTCATAATTGCAGGCCAAACCACTACTGCTGCTGCTATGATGTGGAGTATTAAGTTCCTGGACGAGAACAGAGAGGCCCAAGAAAGACTGCGGGTAAGATCCAAAACCTGTCAAGTTACTTTGAAAGAATGCTAATAAATGCTACCCATAATCCACGACTCTTCAAACTTTTCCTGTGAGGTGAATATTAAAACCCATTTTAAATCTCCAGGAAGAACAATTGTCAATTCTACGAAAGAAGGAAGATGGAGAATTGCTTTCCCTTGAAGATCTTAACAAAATGTACTATGGTTCAAAGGTTATAAGCAACCTAAATTTCTTCTCATTTATAAGAAGTATATAGAGTGCTTCGGATTGCAAACAATTCCATGTACAGTGTATGAAGTGGCCATTGACAGCTTAATTCATGTATATTGCAGGTCGTCAAAGAGACGTTGAGAATGTCCAATGTTTTGGTGTGGTTTCCTCGTGTAGCACTAAGGGATTGCAGATTAGAAGGCACGTTTCTCCTATTATATTCATTCTCTCTCTTTTTGATATTTCACTAATGGAAGCAAATGAGTAAGGTTTTCTACTGTAGACAAGGAAGACGATTCCTGTAGAATTTTTAGAAATATACGCATATACGTTATAAAGAAAAACATTATATTCTATTGGTAAATAGATAGAAGGGATGTCATGTGAACTTGAAGTGCAGTATCTGAATAAATTGATTCGACAGAGGGAACCAGGAACCCCATCCAACTCATTTTGCACGTGATATGCAGGTTTTGAAATAAAGAAAGGATGGCATGTGAACATCGATGCAACATGCATTCATTATGACCCAGATTTGAACAAGGACCCCATGCAATTCAACCCTTCCAGATTTGATGTATCTACCTACAATCAATCATTGTTTGTTCTTTTTTCCTTATCTTCGGTGACTAAACATATATCGTTCATGCACTTATGGTGAATAACTCCAGGAAATTCAAAAGCCATACAGTTTCATACCTTTTGGATCTGGACCCAGGACCTGCCTAGGAATTAACATGGCCAAATTAACAATGTTGGTCTTCTTGCATAGAATGACTGGAGAGTACAAGTGAGTCCCTACATTCCTAAAGGCAGACATTTCATAATGTTCCATGGTATATCATTCAGGATTCTTCTTCTTCTTATCCTTATCATAATTATTTTGCTATTGTATTGTTTAGGTGGACGGTTGATGATCCAGATCCTTGCCTTGAAAAGAAGGCACATATCCCTAGGTTGAGAAGCGGCTGTCCCATTACTTTGAAGACCTTGTAAGATAGGACATACGCATAGATGTGAGACCTCTATCTCAGATATAGGTAGGAGAAGTGACACGATTCTAAAGCATGATAATAGGATAGGTTAAATGTCTTAAAATTTGAGGAAGCAGGTGATTATATGTATCGTCATGGCCTCTAATTACCCACCCTTTATGCTTCATTTAAAAGCAAACCAGAAAAGAAAAAAGAAAAAAAATGAAGTTTGTAATGCAGTGGGAACTCCTTACTCTATGATGTATACTATCTTCTTTGATAAAGAACTATTATTGGAGTCTATACACTTTTCAAAAAAAAAAAAATTATTGGAGTCTATACAGAATTCAATATAATTTGAAGAGGAAAAAAAATCTCAC

mRNA sequence

ATGCTGTGGAAGGAAGAACTTGTGTTCTTCGCTCAAAATTATTTTGACACTGCAGTGGCGGCAGTTCTTTCCATAGCTATTCTAGTGTTAACTTCGAAAGTTTGCTGGAGGTTGAGGAGCAAGAATGTTGGAGGAATTCCTGGTCACTTGGGGTTACCTTTTGTAGGGGAAACCTTCTCTTTTCTTTCTGCCAATAACAGTACCAAAGGGTGTTACCACTTTGTCAGGCTCCGCCGGTTATGGTGGTTCAAGACAAGGCTATTTGGAAAGGTCCACGTGTTTGTTCCAAGTACAGAAGGGGCAAAAGCAATATTTGCCAATGATTTTGTCCAATTCAACAAGGGGTATGTGAAATCCATGGCAGATGCTGTTGGAGAGAAGAGTTTACTTTGCGTTTCCCATGAGAACCACAAGAGGATTAGGCGTCTTCTAGCTGATCCTTTCTCCATGACTTCTTTGTCCAAGTTTGTTAAAGAATTTGACGACATGCTCTCCAAAAGGCTAAAGAAACTAGTAAAAGATGGGAAAAGTTTTGTACTTCTTGATTTTAGCATGAAGATAACATTCGATGCCATGTGCAATATGCTAATGAGTCTAAGAGATGAATCTACGCTCAGACAGATTGAAAAAGACTGTATAGCAGTCTCTGATGCCATGTTATCTTTCCCATTCATGATTCCAGGAACTAGGTACTACAAAGGCATCAAGGCACGAAACCGACTCATGGAAAGGTTTAGAGAGATAATTTCCAGGCGGCGGAGGGGAGAGGAGTCACCAGAAGACTTTCTGCAATCAATGTTGCAGAAAGATTCATATCCTCCTGATGAAAAGCTGGATGACTCAGAGATAATGGATAACCTTTTAACACTCATAATTGCAGGCCAAACCACTACTGCTGCTGCTATGATGTGGAGTATTAAGTTCCTGGACGAGAACAGAGAGGCCCAAGAAAGACTGCGGGAAGAACAATTGTCAATTCTACGAAAGAAGGAAGATGGAGAATTGCTTTCCCTTGAAGATCTTAACAAAATGTACTATGGTTCAAAGGTCGTCAAAGAGACGTTGAGAATGTCCAATGTTTTGGTGTGGTTTCCTCGTGTAGCACTAAGGGATTGCAGATTAGAAGGCACGTTTTCTACTGTAGACAAGGAAGACGATTCCTGTTTTGAAATAAAGAAAGGATGGCATGTGAACATCGATGCAACATGCATTCATTATGACCCAGATTTGAACAAGGACCCCATGCAATTCAACCCTTCCAGATTTGATGAAATTCAAAAGCCATACAGTTTCATACCTTTTGGATCTGGACCCAGGACCTGCCTAGGAATTAACATGGCCAAATTAACAATGTTGGTCTTCTTGCATAGAATGACTGGAGAGTACAAGTGGACGGTTGATGATCCAGATCCTTGCCTTGAAAAGAAGGCACATATCCCTAGGTTGAGAAGCGGCTGTCCCATTACTTTGAAGACCTTGTAA

Coding sequence (CDS)

ATGCTGTGGAAGGAAGAACTTGTGTTCTTCGCTCAAAATTATTTTGACACTGCAGTGGCGGCAGTTCTTTCCATAGCTATTCTAGTGTTAACTTCGAAAGTTTGCTGGAGGTTGAGGAGCAAGAATGTTGGAGGAATTCCTGGTCACTTGGGGTTACCTTTTGTAGGGGAAACCTTCTCTTTTCTTTCTGCCAATAACAGTACCAAAGGGTGTTACCACTTTGTCAGGCTCCGCCGGTTATGGTGGTTCAAGACAAGGCTATTTGGAAAGGTCCACGTGTTTGTTCCAAGTACAGAAGGGGCAAAAGCAATATTTGCCAATGATTTTGTCCAATTCAACAAGGGGTATGTGAAATCCATGGCAGATGCTGTTGGAGAGAAGAGTTTACTTTGCGTTTCCCATGAGAACCACAAGAGGATTAGGCGTCTTCTAGCTGATCCTTTCTCCATGACTTCTTTGTCCAAGTTTGTTAAAGAATTTGACGACATGCTCTCCAAAAGGCTAAAGAAACTAGTAAAAGATGGGAAAAGTTTTGTACTTCTTGATTTTAGCATGAAGATAACATTCGATGCCATGTGCAATATGCTAATGAGTCTAAGAGATGAATCTACGCTCAGACAGATTGAAAAAGACTGTATAGCAGTCTCTGATGCCATGTTATCTTTCCCATTCATGATTCCAGGAACTAGGTACTACAAAGGCATCAAGGCACGAAACCGACTCATGGAAAGGTTTAGAGAGATAATTTCCAGGCGGCGGAGGGGAGAGGAGTCACCAGAAGACTTTCTGCAATCAATGTTGCAGAAAGATTCATATCCTCCTGATGAAAAGCTGGATGACTCAGAGATAATGGATAACCTTTTAACACTCATAATTGCAGGCCAAACCACTACTGCTGCTGCTATGATGTGGAGTATTAAGTTCCTGGACGAGAACAGAGAGGCCCAAGAAAGACTGCGGGAAGAACAATTGTCAATTCTACGAAAGAAGGAAGATGGAGAATTGCTTTCCCTTGAAGATCTTAACAAAATGTACTATGGTTCAAAGGTCGTCAAAGAGACGTTGAGAATGTCCAATGTTTTGGTGTGGTTTCCTCGTGTAGCACTAAGGGATTGCAGATTAGAAGGCACGTTTTCTACTGTAGACAAGGAAGACGATTCCTGTTTTGAAATAAAGAAAGGATGGCATGTGAACATCGATGCAACATGCATTCATTATGACCCAGATTTGAACAAGGACCCCATGCAATTCAACCCTTCCAGATTTGATGAAATTCAAAAGCCATACAGTTTCATACCTTTTGGATCTGGACCCAGGACCTGCCTAGGAATTAACATGGCCAAATTAACAATGTTGGTCTTCTTGCATAGAATGACTGGAGAGTACAAGTGGACGGTTGATGATCCAGATCCTTGCCTTGAAAAGAAGGCACATATCCCTAGGTTGAGAAGCGGCTGTCCCATTACTTTGAAGACCTTGTAA

Protein sequence

MLWKEELVFFAQNYFDTAVAAVLSIAILVLTSKVCWRLRSKNVGGIPGHLGLPFVGETFSFLSANNSTKGCYHFVRLRRLWWFKTRLFGKVHVFVPSTEGAKAIFANDFVQFNKGYVKSMADAVGEKSLLCVSHENHKRIRRLLADPFSMTSLSKFVKEFDDMLSKRLKKLVKDGKSFVLLDFSMKITFDAMCNMLMSLRDESTLRQIEKDCIAVSDAMLSFPFMIPGTRYYKGIKARNRLMERFREIISRRRRGEESPEDFLQSMLQKDSYPPDEKLDDSEIMDNLLTLIIAGQTTTAAAMMWSIKFLDENREAQERLREEQLSILRKKEDGELLSLEDLNKMYYGSKVVKETLRMSNVLVWFPRVALRDCRLEGTFSTVDKEDDSCFEIKKGWHVNIDATCIHYDPDLNKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGEYKWTVDDPDPCLEKKAHIPRLRSGCPITLKTL
Homology
BLAST of Spg012884 vs. NCBI nr
Match: XP_022950585.1 (abscisic acid 8'-hydroxylase 3-like isoform X2 [Cucurbita moschata] >KAG7034125.1 Abscisic acid 8'-hydroxylase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 894.8 bits (2311), Expect = 3.3e-256
Identity = 447/497 (89.94%), Postives = 463/497 (93.16%), Query Frame = 0

Query: 1   MLWKEELVFFAQNYFD-TAVAAVLSIAILVLTSKVCWRLRSKNVGGIPGHLGLPFVGETF 60
           MLWKEELV  AQNYFD T VAAVLSI ILVL SKVCWR RSKNVGGIPGHLGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSIGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 61  SFLSANNSTKGCYHFVRLRRLW---WFKTRLFGKVHVFVPSTEGAKAIFANDFVQFNKGY 120
           SFLSANNST+GCY FVRLRRLW   WF+TRLFGK+HVFVPS EGAKAI ANDFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 121 VKSMADAVGEKSLLCVSHENHKRIRRLLADPFSMTSLSKFVKEFDDMLSKRLKKLVKDGK 180
           VKSM DAVGEKSLLCVSHE+H+RIRRLLADPFSMTSLSKFVK+FDDMLSKRL+KLVKDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 181 SFVLLDFSMKITFDAMCNMLMSLRDESTLRQIEKDCIAVSDAMLSFPFMIPGTRYYKGIK 240
           SFVLLDFSMKITFD+MCNMLMSLRDESTLRQIEKDC AVSDAMLS PFMIPGTRYYKGIK
Sbjct: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCSAVSDAMLSLPFMIPGTRYYKGIK 240

Query: 241 ARNRLMERFREIISRRRRGEESPEDFLQSMLQKDSYPPDEKLDDSEIMDNLLTLIIAGQT 300
           ARNRLMER REIISRRR+GEESPEDFLQSMLQ+DSYPPD+KLDDSEIMDNLLTLIIA QT
Sbjct: 241 ARNRLMERLREIISRRRKGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300

Query: 301 TTAAAMMWSIKFLDENREAQERLREEQLSILRKKEDGELLSLEDLNKMYYGSKVVKETLR 360
           TTAAAMMWS+KFLDENREAQERLREEQLSILRKKEDGELL+LEDLNKM +GSKVVKETLR
Sbjct: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360

Query: 361 MSNVLVWFPRVALRDCRLEGTFSTVDKEDDSCFEIKKGWHVNIDATCIHYDPDLNKDPMQ 420
           MSNVL+WFPRVAL DCRLEG            FEIKKGWHVNIDATCIHYDPDL KDPMQ
Sbjct: 361 MSNVLLWFPRVALGDCRLEG------------FEIKKGWHVNIDATCIHYDPDLYKDPMQ 420

Query: 421 FNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGEYKWTVDDPDPCLEKK 480
           FNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTG Y+WTVDDPDPCLEKK
Sbjct: 421 FNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKK 480

Query: 481 AHIPRLRSGCPITLKTL 494
           AHIPRLRSGCPITLKTL
Sbjct: 481 AHIPRLRSGCPITLKTL 485

BLAST of Spg012884 vs. NCBI nr
Match: XP_022978466.1 (abscisic acid 8'-hydroxylase 3-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 894.4 bits (2310), Expect = 4.3e-256
Identity = 446/497 (89.74%), Postives = 463/497 (93.16%), Query Frame = 0

Query: 1   MLWKEELVFFAQNYFD-TAVAAVLSIAILVLTSKVCWRLRSKNVGGIPGHLGLPFVGETF 60
           MLWKEELV  AQNYFD T VAAVLS+ ILVL SKVCWR RSKNVGGIPGHLGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 61  SFLSANNSTKGCYHFVRLRRLW---WFKTRLFGKVHVFVPSTEGAKAIFANDFVQFNKGY 120
           SFLSANNST+GCY FVRLRRLW   WF+TRLFGK+HVFVPS EGAKAI ANDFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 121 VKSMADAVGEKSLLCVSHENHKRIRRLLADPFSMTSLSKFVKEFDDMLSKRLKKLVKDGK 180
           VKSM DAVGEKSLLCVSHE+H+RIRRLLADPFSMTSLSKFVK+FDDMLSKRL+KLVKDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 181 SFVLLDFSMKITFDAMCNMLMSLRDESTLRQIEKDCIAVSDAMLSFPFMIPGTRYYKGIK 240
           SFVLLDFSMKITFD+MCNMLMSLRDESTLRQIEKDC AV+DAMLSFPFMIPGTRYYKGIK
Sbjct: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVTDAMLSFPFMIPGTRYYKGIK 240

Query: 241 ARNRLMERFREIISRRRRGEESPEDFLQSMLQKDSYPPDEKLDDSEIMDNLLTLIIAGQT 300
           ARNRLMER  EIISRRRRGEESPEDFLQSMLQ+DSYPPD+KLDDSEIMDNLLTLIIA QT
Sbjct: 241 ARNRLMERLGEIISRRRRGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300

Query: 301 TTAAAMMWSIKFLDENREAQERLREEQLSILRKKEDGELLSLEDLNKMYYGSKVVKETLR 360
           TTAAAMMWS+KFLDENREAQERLREEQLSILRKKEDGELL+LEDLNKM +GSKVVKETLR
Sbjct: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360

Query: 361 MSNVLVWFPRVALRDCRLEGTFSTVDKEDDSCFEIKKGWHVNIDATCIHYDPDLNKDPMQ 420
           MSNVL+WFPRVAL DCRLEG            FEIKKGWHVNIDATCIHYDPDL KDPMQ
Sbjct: 361 MSNVLLWFPRVALGDCRLEG------------FEIKKGWHVNIDATCIHYDPDLYKDPMQ 420

Query: 421 FNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGEYKWTVDDPDPCLEKK 480
           FNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTG Y+WTVDDPDPCLEKK
Sbjct: 421 FNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKK 480

Query: 481 AHIPRLRSGCPITLKTL 494
           AHIPRLRSGCPITLKTL
Sbjct: 481 AHIPRLRSGCPITLKTL 485

BLAST of Spg012884 vs. NCBI nr
Match: XP_023543351.1 (abscisic acid 8'-hydroxylase 3-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 894.0 bits (2309), Expect = 5.7e-256
Identity = 447/497 (89.94%), Postives = 462/497 (92.96%), Query Frame = 0

Query: 1   MLWKEELVFFAQNYFD-TAVAAVLSIAILVLTSKVCWRLRSKNVGGIPGHLGLPFVGETF 60
           MLWKEELV  AQNYFD T VAAVLSI ILVL SKVCWR RSKNVGGIPGHLGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSIGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 61  SFLSANNSTKGCYHFVRLRRLW---WFKTRLFGKVHVFVPSTEGAKAIFANDFVQFNKGY 120
           SFLSANNST+GCY FVRLRRLW   WF+TRLFGK+HVFVPS EGAKAI ANDFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 121 VKSMADAVGEKSLLCVSHENHKRIRRLLADPFSMTSLSKFVKEFDDMLSKRLKKLVKDGK 180
           VKSM DAVGEKSLLCVSHE+H+RIRRLLADPFSMTSLSKFVK+FDDMLSKRL+KLVKDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 181 SFVLLDFSMKITFDAMCNMLMSLRDESTLRQIEKDCIAVSDAMLSFPFMIPGTRYYKGIK 240
           SFVLLDFSMKITFD+MCNMLMSLRDESTLRQIEKDC AVSDAMLSFPFMIPGTRYYKGIK
Sbjct: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVSDAMLSFPFMIPGTRYYKGIK 240

Query: 241 ARNRLMERFREIISRRRRGEESPEDFLQSMLQKDSYPPDEKLDDSEIMDNLLTLIIAGQT 300
           ARNRLMER REIISRRR GEESPEDFLQSMLQ+DSYPPD+KLDDSEIMDNLLTLIIA QT
Sbjct: 241 ARNRLMERLREIISRRRSGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300

Query: 301 TTAAAMMWSIKFLDENREAQERLREEQLSILRKKEDGELLSLEDLNKMYYGSKVVKETLR 360
           TTAAAM WS+KFLDENREAQERLREEQLSILRKKEDGELL+LEDLNKM +GSKVVKETLR
Sbjct: 301 TTAAAMTWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360

Query: 361 MSNVLVWFPRVALRDCRLEGTFSTVDKEDDSCFEIKKGWHVNIDATCIHYDPDLNKDPMQ 420
           MSNVL+WFPRVAL DCRLEG            FEIKKGWHVNIDATCIHYDPDL KDPMQ
Sbjct: 361 MSNVLLWFPRVALGDCRLEG------------FEIKKGWHVNIDATCIHYDPDLYKDPMQ 420

Query: 421 FNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGEYKWTVDDPDPCLEKK 480
           FNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTG Y+WTVDDPDPCLEKK
Sbjct: 421 FNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKK 480

Query: 481 AHIPRLRSGCPITLKTL 494
           AHIPRLRSGCPITLKTL
Sbjct: 481 AHIPRLRSGCPITLKTL 485

BLAST of Spg012884 vs. NCBI nr
Match: KAG6603947.1 (Abscisic acid 8'-hydroxylase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 890.6 bits (2300), Expect = 6.3e-255
Identity = 446/497 (89.74%), Postives = 462/497 (92.96%), Query Frame = 0

Query: 1   MLWKEELVFFAQNYFD-TAVAAVLSIAILVLTSKVCWRLRSKNVGGIPGHLGLPFVGETF 60
           MLWKEELV  AQNYFD T VAAVLSI ILVL SKVCWR RSKNVGGIPGHLGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSIGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 61  SFLSANNSTKGCYHFVRLRRLW---WFKTRLFGKVHVFVPSTEGAKAIFANDFVQFNKGY 120
           SFLSANNST+GCY FVRLRRLW   WF+TRLFGK+HVFVPS EGAKAI ANDFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 121 VKSMADAVGEKSLLCVSHENHKRIRRLLADPFSMTSLSKFVKEFDDMLSKRLKKLVKDGK 180
           VKSM DAVGEKSLLCVS E+H+RIRRLLADPFSMTSLSKFVK+FDDMLSKRL+KLVKDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSLEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 181 SFVLLDFSMKITFDAMCNMLMSLRDESTLRQIEKDCIAVSDAMLSFPFMIPGTRYYKGIK 240
           SFVLLDFSMKITFD+MCNMLMSLRDESTLRQIEKDC AVSDAMLS PFMIPGTRYYKGIK
Sbjct: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCSAVSDAMLSLPFMIPGTRYYKGIK 240

Query: 241 ARNRLMERFREIISRRRRGEESPEDFLQSMLQKDSYPPDEKLDDSEIMDNLLTLIIAGQT 300
           ARNRLMER REIISRRR+GEESPEDFLQSMLQ+DSYPPD+KLDDSEIMDNLLTLIIA QT
Sbjct: 241 ARNRLMERLREIISRRRKGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300

Query: 301 TTAAAMMWSIKFLDENREAQERLREEQLSILRKKEDGELLSLEDLNKMYYGSKVVKETLR 360
           TTAAAMMWS+KFLDENREAQERLREEQLSILRKKEDGELL+LEDLNKM +GSKVVKETLR
Sbjct: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360

Query: 361 MSNVLVWFPRVALRDCRLEGTFSTVDKEDDSCFEIKKGWHVNIDATCIHYDPDLNKDPMQ 420
           MSNVL+WFPRVAL DCRLEG            FEIKKGWHVNIDATCIHYDPDL KDPMQ
Sbjct: 361 MSNVLLWFPRVALGDCRLEG------------FEIKKGWHVNIDATCIHYDPDLYKDPMQ 420

Query: 421 FNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGEYKWTVDDPDPCLEKK 480
           FNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTG Y+WTVDDPDPCLEKK
Sbjct: 421 FNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKK 480

Query: 481 AHIPRLRSGCPITLKTL 494
           AHIPRLRSGCPITLKTL
Sbjct: 481 AHIPRLRSGCPITLKTL 485

BLAST of Spg012884 vs. NCBI nr
Match: XP_038883385.1 (abscisic acid 8'-hydroxylase 3-like [Benincasa hispida] >XP_038883386.1 abscisic acid 8'-hydroxylase 3-like [Benincasa hispida])

HSP 1 Score: 887.9 bits (2293), Expect = 4.1e-254
Identity = 446/496 (89.92%), Postives = 460/496 (92.74%), Query Frame = 0

Query: 1   MLWKEELVFFAQNYFDTAVAAVLSIAILVLTSKVCWRLRSKNVGGIPGHLGLPFVGETFS 60
           ML KE LVF AQNYFD  VAA+LSIAIL L SKVCWR RSKNVGGIPG+LGLPFVGETFS
Sbjct: 1   MLCKEGLVFLAQNYFDPTVAALLSIAILALVSKVCWRQRSKNVGGIPGNLGLPFVGETFS 60

Query: 61  FLSANNSTKGCYHFVRLRRLW---WFKTRLFGKVHVFVPSTEGAKAIFANDFVQFNKGYV 120
           FLSANNST+GCYHFVRLRRLW   WFKTRLFGK+HVFVPS EGAKAIFANDFVQFNKGYV
Sbjct: 61  FLSANNSTRGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFANDFVQFNKGYV 120

Query: 121 KSMADAVGEKSLLCVSHENHKRIRRLLADPFSMTSLSKFVKEFDDMLSKRLKKLVKDGKS 180
           KSM DAVGEKSLLCVSHENH+RIRRLLADPFSMTSLS FVKEFDDMLSKRLKKLVKDGKS
Sbjct: 121 KSMGDAVGEKSLLCVSHENHRRIRRLLADPFSMTSLSNFVKEFDDMLSKRLKKLVKDGKS 180

Query: 181 FVLLDFSMKITFDAMCNMLMSLRDESTLRQIEKDCIAVSDAMLSFPFMIPGTRYYKGIKA 240
           FVLLDFSMKITFD+MC+MLMSLRDESTLRQIEKDCIAVSDAMLSFP MIP TRYYKGIKA
Sbjct: 181 FVLLDFSMKITFDSMCHMLMSLRDESTLRQIEKDCIAVSDAMLSFPVMIPETRYYKGIKA 240

Query: 241 RNRLMERFREIISRRRRGEESPEDFLQSMLQKDSYPPDEKLDDSEIMDNLLTLIIAGQTT 300
           RNRLM+RFREII RRRRGEESP+DFLQSMLQ+DSYP +EKLDDSEIMDNLLTLIIAGQTT
Sbjct: 241 RNRLMKRFREIIFRRRRGEESPKDFLQSMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT 300

Query: 301 TAAAMMWSIKFLDENREAQERLREEQLSILRKKEDGELLSLEDLNKMYYGSKVVKETLRM 360
           TAAAMMWSIKFLDENREAQERLREEQLSILR KEDGELL+LEDLNKM YGSKVVKETLRM
Sbjct: 301 TAAAMMWSIKFLDENREAQERLREEQLSILRNKEDGELLTLEDLNKMSYGSKVVKETLRM 360

Query: 361 SNVLVWFPRVALRDCRLEGTFSTVDKEDDSCFEIKKGWHVNIDATCIHYDPDLNKDPMQF 420
           SNVL+WFPRVAL DCRLEG            FEIK+GWHVNIDATCIHYDPDL KDPMQF
Sbjct: 361 SNVLLWFPRVALGDCRLEG------------FEIKEGWHVNIDATCIHYDPDLYKDPMQF 420

Query: 421 NPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGEYKWTVDDPDPCLEKKA 480
            PSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTG YKWTVDDPDPCLEKKA
Sbjct: 421 TPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYKWTVDDPDPCLEKKA 480

Query: 481 HIPRLRSGCPITLKTL 494
           HIPRLRSGCPITLKTL
Sbjct: 481 HIPRLRSGCPITLKTL 484

BLAST of Spg012884 vs. ExPASy Swiss-Prot
Match: Q50EK1 (Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 3.5e-51
Identity = 150/466 (32.19%), Postives = 223/466 (47.85%), Query Frame = 0

Query: 39  RSKNVGGI-PGHLGLPFVGETFSFLSANNSTKGCYHFVRLRRLWW--FKTRLFGKVHVFV 98
           R+K   GI PG  G P +GET  FL           F    + +   F T L G   V  
Sbjct: 44  RTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNPRDFFDSRTQKYGNVFTTSLVGHPTVVF 103

Query: 99  PSTEGAKAIFANDFVQFNKGYVKSMADAVG---EKSLLCVSHENHKRIRRLLADPFSMTS 158
            S EG + +F+N+    NK  V S   +VG     SL+    ++ KR+RR+L       +
Sbjct: 104 CSPEGNRFLFSNE----NKLVVNSWPSSVGNLFRSSLITTVGDDAKRLRRILMTFLRPEA 163

Query: 159 LSKFVKEFDDMLSKRLKKLVKDGKSFVLLDFSMKITFDAMCNMLMSLRDESTLRQIEKDC 218
           L +FV   D M  + L +        + L    + TF   C++  S+  +  L ++    
Sbjct: 164 LREFVGRVDSMTKRHLAEHWIGKDEVMALPLLKRYTFSLACDLFASINTKDDLDRLWLHF 223

Query: 219 IAVSDAMLSFPFMIPGTRYYKGIKARNRLMERFREIISRRR----RGEESPEDFLQSMLQ 278
           +     ++  P  +PGTRY K   A N + ++   II+ R+     G  SPE  L S L 
Sbjct: 224 MVFVKGVMQIPIDLPGTRYNKTKHAANAIRQQLGSIINERKIALEAGNASPEQDLLSFLL 283

Query: 279 KDSYPPDEKLDDSEIMDNLLTLIIAGQTTTAAAMMWSIKFLDENREAQERLREEQLSILR 338
            +     E L D+EI DN+L L+ AG  T+++ +   +KFL EN    E +  EQL+I  
Sbjct: 284 SNVDEQGESLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFLAENPHCYEEVLREQLNIAG 343

Query: 339 KKEDGELLSLEDLNKMYYGSKVVKETLRMSNVLVWFPRVALRDCRLEGTFSTVDKE-DDS 398
            KE+G+LL  EDL +M Y  +V +E LR+      FP V       +G+F    KE    
Sbjct: 344 SKEEGQLLEWEDLQRMKYSWRVAQEALRL------FPAV-------QGSFRKAIKEFIYD 403

Query: 399 CFEIKKGWHVNIDATCIHYDPDLNKDPMQFNPSRFD-EIQKPYSFIPFGSGPRTCLGINM 458
            F I KGW ++      H   +   +P +F+PSRF+ E   PY+F+PFG GPR C G   
Sbjct: 404 GFTIPKGWKLHWTVNSTHQKSEYFSNPEKFDPSRFEGEGPPPYTFVPFGGGPRMCPGNEF 463

Query: 459 AKLTMLVFLHRMTGEYKWTVDDPDPCLEKKA--HIPRLRSGCPITL 491
           A++ +L+FLH +   + W + +P   LEK     +P   +G PI L
Sbjct: 464 ARMEILIFLHNIVKNFNWNLVNP---LEKVIVDPMPAPVNGLPIKL 489

BLAST of Spg012884 vs. ExPASy Swiss-Prot
Match: Q9FH76 (Abscisic acid 8'-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=CYP707A3 PE=1 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 7.9e-51
Identity = 142/479 (29.65%), Postives = 236/479 (49.27%), Query Frame = 0

Query: 15  FDTAVAAVLSIAILVLTSKVCWRLRSKNVGGIPGHLGLPFVGETFSFLSANNSTKGCYHF 74
           F T  AA L + +L   + V  R  S  +   PG +G P+VGETF   S + +    +  
Sbjct: 7   FLTLSAAALFLCLLRFIAGV-RRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNV---FFA 66

Query: 75  VRLRRLW-WFKTRLFGKVHVFVPSTEGAKAIFANDFVQFNKGYVKSMADAVGEKSLLCVS 134
            + RR    FKT + G   V + S E AK +       F   +  S    +G++++    
Sbjct: 67  AKQRRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQ 126

Query: 135 HENHKRIRRLLADPFSMTSLSKFVKEFDDMLSKRLKKLVKDGKSFVLLDFSMKITFDAMC 194
            + H ++R+L+   F   ++   V   + +  + L     DG            TF+   
Sbjct: 127 GDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSW--DGTQLNTYQEMKTYTFNVAL 186

Query: 195 NMLMSLRDESTLRQIEKDCIAVSD-AMLSFPFMIPGTRYYKGIKARNRLMERFREIISRR 254
             ++  +DE   R+  K C  + +    S P  +PGT ++K +KAR  L +    I+S+R
Sbjct: 187 ISILG-KDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKR 246

Query: 255 RRGEESPEDFLQSMLQKDSYPPDEKLDDSEIMDNLLTLIIAGQTTTAAAMMWSIKFLDEN 314
           R+   S  D L S ++  +      L D +I DN++ +I A + TTA+ + W +K+L +N
Sbjct: 247 RQNPSSHTDLLGSFMEDKA-----GLTDEQIADNIIGVIFAARDTTASVLTWILKYLADN 306

Query: 315 REAQERLREEQLSILRKKEDGELLSLEDLNKMYYGSKVVKETLRMSNVLVWFPRVALRDC 374
               E + EEQ++I + K++GE L+ ED  KM    +V++ETLR + +L +  R A+ D 
Sbjct: 307 PTVLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDV 366

Query: 375 RLEGTFSTVDKEDDSCFEIKKGWHVNIDATCIHYDPDLNKDPMQFNPSRFDEIQKPYSFI 434
             EG            + I KGW V      IH++ D+  DP +F+PSRF+   KP +F+
Sbjct: 367 EYEG------------YLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKPNTFM 426

Query: 435 PFGSGPRTCLGINMAKLTMLVFLHRMTGEYKWTVDDPDPCLEKKAHIPRLRSGCPITLK 492
           PFGSG  +C G  +AKL + V +H +T +Y+W++  P   ++        ++G PI L+
Sbjct: 427 PFGSGIHSCPGNELAKLEISVLIHHLTTKYRWSIVGPSDGIQ-YGPFALPQNGLPIALE 460

BLAST of Spg012884 vs. ExPASy Swiss-Prot
Match: Q949P1 (Abscisic acid 8'-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=CYP707A1 PE=1 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 6.7e-50
Identity = 129/421 (30.64%), Postives = 211/421 (50.12%), Query Frame = 0

Query: 47  PGHLGLPFVGETFSFLSANNSTKGCYHFVRLRRLWWFKTRLFGKVHVFVPSTEGAKAIFA 106
           PG +G P+VGETF   S + +    +   + R    FKT + G   V + S E AK +  
Sbjct: 38  PGTMGWPYVGETFQLYSQDPNV--FFQSKQKRYGSVFKTHVLGCPCVMISSPEAAKFVLV 97

Query: 107 NDFVQFNKGYVKSMADAVGEKSLLCVSHENHKRIRRLLADPFSMTSLSKFVKEFDDMLSK 166
                F   +  S    +G++++     + H ++R+L+   F   S+   V + + +   
Sbjct: 98  TKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPESIRNMVPDIESIAQD 157

Query: 167 RLKKLVKDGKSFVLLDFSMKITFDAMCNMLMSLRDESTLRQIEKDCIAVSD-AMLSFPFM 226
            L+    +G            TF+     +   +DE   R+  K C  + +    S P  
Sbjct: 158 SLRSW--EGTMINTYQEMKTYTFNVALLSIFG-KDEVLYREDLKRCYYILEKGYNSMPVN 217

Query: 227 IPGTRYYKGIKARNRLMERFREIISRRRRGEESPEDFLQSMLQKDSYPPDEKLDDSEIMD 286
           +PGT ++K +KAR  L +    I+S RR+   S  D L S +        E+L D +I D
Sbjct: 218 LPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFM-----GDKEELTDEQIAD 277

Query: 287 NLLTLIIAGQTTTAAAMMWSIKFLDENREAQERLREEQLSILRKKEDGELLSLEDLNKMY 346
           N++ +I A + TTA+ M W +K+L EN    E + EEQ++I + KE+GE L+  D  KM 
Sbjct: 278 NIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKDKEEGESLTWGDTKKMP 337

Query: 347 YGSKVVKETLRMSNVLVWFPRVALRDCRLEGTFSTVDKEDDSCFEIKKGWHVNIDATCIH 406
             S+V++ETLR++++L +  R A+ D   EG            + I KGW V      IH
Sbjct: 338 LTSRVIQETLRVASILSFTFREAVEDVEYEG------------YLIPKGWKVLPLFRNIH 397

Query: 407 YDPDLNKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGEYKWT 466
           +  D+  +P +F+PSRF+   KP +F+PFG+G  +C G  +AKL M + +H +T +Y W+
Sbjct: 398 HSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIMIHHLTTKYSWS 436

BLAST of Spg012884 vs. ExPASy Swiss-Prot
Match: Q50EK0 (Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.1e-49
Identity = 142/442 (32.13%), Postives = 208/442 (47.06%), Query Frame = 0

Query: 39  RSKNVGGI-PGHLGLPFVGETFSFLSANNSTKGCYHFVRLRRLWW--FKTRLFGKVHVFV 98
           R+K   GI PG  G P +GET  FL           F    + +   F T L G   V  
Sbjct: 44  RTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNPRDFFDSRTQKYGNVFTTSLVGHPTVVF 103

Query: 99  PSTEGAKAIFANDFVQFNKGYVKSMADAVG---EKSLLCVSHENHKRIRRLLADPFSMTS 158
            S EG + +F+N+    NK  V S   +VG     SL+    ++ KR+RR+L       +
Sbjct: 104 CSPEGNRFLFSNE----NKLVVNSWPSSVGNLFRSSLITTVGDDAKRLRRILMTFLRPEA 163

Query: 159 LSKFVKEFDDMLSKRLKKLVKDGKSFVLLDFSMKITFDAMCNMLMSLRDESTLRQIEKDC 218
           L +FV   D M  + L +          L    + TF   C++  S+ ++  L ++    
Sbjct: 164 LREFVGRVDSMTKRHLAEHWIGKDEVTALPLLKRYTFSLACDLFASINNKDDLGRLWLHF 223

Query: 219 IAVSDAMLSFPFMIPGTRYYKGIKARNRLMERFREIISRRRRGEE----SPEDFLQSMLQ 278
           +     ++  P  +PGTRY K   A N + ++   II+ R+ G E    SPE  L S L 
Sbjct: 224 MVFVKGVMQIPIDLPGTRYNKAKHAANAIRQQLGSIINERKIGLEAGNASPEQDLLSFLL 283

Query: 279 KDSYPPDEKLDDSEIMDNLLTLIIAGQTTTAAAMMWSIKFLDENREAQERLREEQLSILR 338
            +     E L D+EI DN+L L+ AG  T+++ +   +KFL EN    E +  EQL I  
Sbjct: 284 SNVDEQGESLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFLAENPHCYEEVLREQLDIAG 343

Query: 339 KKEDGELLSLEDLNKMYYGSKVVKETLRMSNVLVWFPRVALRDCRLEGTFSTVDKEDDSC 398
            KE G+LL  EDL +M Y  +V +E LR+        R A+++   +G            
Sbjct: 344 SKEAGQLLEWEDLQRMKYSWRVAQEALRLFPAAQGSFRKAIKEFIYDG------------ 403

Query: 399 FEIKKGWHVNIDATCIHYDPDLNKDPMQFNPSRFD-EIQKPYSFIPFGSGPRTCLGINMA 458
           F I KGW +       H   +   +P  F+PSRF+ E   PY+F+PFG GPR C G   A
Sbjct: 404 FTIPKGWKMYWTVNSTHRKSEYFSNPETFDPSRFEGEGPPPYTFVPFGGGPRMCPGNEFA 463

Query: 459 KLTMLVFLHRMTGEYKWTVDDP 470
           +L +LVFLH +    KW + +P
Sbjct: 464 RLEILVFLHNIVKNCKWNLVNP 469

BLAST of Spg012884 vs. ExPASy Swiss-Prot
Match: O81077 (Abscisic acid 8'-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=CYP707A2 PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.6e-48
Identity = 136/466 (29.18%), Postives = 229/466 (49.14%), Query Frame = 0

Query: 9   FFAQNYFDTAVAAVLSIAILVLTSKVCWRL--RSKNVGGIPGHLGLPFVGETFSFLSANN 68
           FF+  Y D      L+I ++V+     W L  + + +   PG +GLP++GET    + N 
Sbjct: 11  FFSSLYADEPALITLTIVVVVVVLLFKWWLHWKEQRLRLPPGSMGLPYIGETLRLYTENP 70

Query: 69  STKGCYHFVRLRRLWW---FKTRLFGKVHVFVPSTEGAKAIFANDFVQFNKGYVKSMADA 128
           ++     F   R+  +   FKT + G   V + S E A+ +  +    F   Y  S    
Sbjct: 71  NS-----FFATRQNKYGDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERM 130

Query: 129 VGEKSLLCVSHENHKRIRRLLADPFSMTSLSKFVKEFDDMLSKRLKKLVKDGKSFVLLDF 188
           +G ++L       H  ++RL+   F  ++L   V   + ++ + L       KS   L++
Sbjct: 131 IGPEALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVLQTLSSWTSQ-KSINTLEY 190

Query: 189 SMKITFD-AMCNMLMSLRDESTLRQIEKDCIAVSDAMLSFPFMIPGTRYYKGIKARNRLM 248
             +  FD A+ +      + +T+  I+     +     S P  +PGT ++K +KAR  L 
Sbjct: 191 MKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELS 250

Query: 249 ERFREIISRRRRGEESPEDFLQSML-QKDSYPPDEKLDDSEIMDNLLTLIIAGQTTTAAA 308
           E  R++I +RR         L  +L  KD       L DS+I DN++ +I A   TTA+ 
Sbjct: 251 EELRKVIEKRRENGREEGGLLGVLLGAKDQ--KRNGLSDSQIADNIIGVIFAATDTTASV 310

Query: 309 MMWSIKFLDENREAQERLREEQLSILRK-KEDGELLSLEDLNKMYYGSKVVKETLRMSNV 368
           + W +K+L ++    + +  EQ SI +K K++   +S ED  KM   ++V++ETLR ++V
Sbjct: 311 LTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASV 370

Query: 369 LVWFPRVALRDCRLEGTFSTVDKEDDSCFEIKKGWHVNIDATCIHYDPDLNKDPMQFNPS 428
           L +  R A++D   +G            + I KGW V      IH+  +   DP +F+PS
Sbjct: 371 LSFTFREAVQDVEYDG------------YLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPS 430

Query: 429 RFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGEYKWTV 467
           RF+   KPY+++PFG+G  +C G  +AKL ML+ LH +T  ++W V
Sbjct: 431 RFEVAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEV 456

BLAST of Spg012884 vs. ExPASy TrEMBL
Match: A0A6J1GG59 (abscisic acid 8'-hydroxylase 3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453639 PE=3 SV=1)

HSP 1 Score: 894.8 bits (2311), Expect = 1.6e-256
Identity = 447/497 (89.94%), Postives = 463/497 (93.16%), Query Frame = 0

Query: 1   MLWKEELVFFAQNYFD-TAVAAVLSIAILVLTSKVCWRLRSKNVGGIPGHLGLPFVGETF 60
           MLWKEELV  AQNYFD T VAAVLSI ILVL SKVCWR RSKNVGGIPGHLGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSIGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 61  SFLSANNSTKGCYHFVRLRRLW---WFKTRLFGKVHVFVPSTEGAKAIFANDFVQFNKGY 120
           SFLSANNST+GCY FVRLRRLW   WF+TRLFGK+HVFVPS EGAKAI ANDFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 121 VKSMADAVGEKSLLCVSHENHKRIRRLLADPFSMTSLSKFVKEFDDMLSKRLKKLVKDGK 180
           VKSM DAVGEKSLLCVSHE+H+RIRRLLADPFSMTSLSKFVK+FDDMLSKRL+KLVKDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 181 SFVLLDFSMKITFDAMCNMLMSLRDESTLRQIEKDCIAVSDAMLSFPFMIPGTRYYKGIK 240
           SFVLLDFSMKITFD+MCNMLMSLRDESTLRQIEKDC AVSDAMLS PFMIPGTRYYKGIK
Sbjct: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCSAVSDAMLSLPFMIPGTRYYKGIK 240

Query: 241 ARNRLMERFREIISRRRRGEESPEDFLQSMLQKDSYPPDEKLDDSEIMDNLLTLIIAGQT 300
           ARNRLMER REIISRRR+GEESPEDFLQSMLQ+DSYPPD+KLDDSEIMDNLLTLIIA QT
Sbjct: 241 ARNRLMERLREIISRRRKGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300

Query: 301 TTAAAMMWSIKFLDENREAQERLREEQLSILRKKEDGELLSLEDLNKMYYGSKVVKETLR 360
           TTAAAMMWS+KFLDENREAQERLREEQLSILRKKEDGELL+LEDLNKM +GSKVVKETLR
Sbjct: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360

Query: 361 MSNVLVWFPRVALRDCRLEGTFSTVDKEDDSCFEIKKGWHVNIDATCIHYDPDLNKDPMQ 420
           MSNVL+WFPRVAL DCRLEG            FEIKKGWHVNIDATCIHYDPDL KDPMQ
Sbjct: 361 MSNVLLWFPRVALGDCRLEG------------FEIKKGWHVNIDATCIHYDPDLYKDPMQ 420

Query: 421 FNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGEYKWTVDDPDPCLEKK 480
           FNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTG Y+WTVDDPDPCLEKK
Sbjct: 421 FNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKK 480

Query: 481 AHIPRLRSGCPITLKTL 494
           AHIPRLRSGCPITLKTL
Sbjct: 481 AHIPRLRSGCPITLKTL 485

BLAST of Spg012884 vs. ExPASy TrEMBL
Match: A0A6J1IMR3 (abscisic acid 8'-hydroxylase 3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111478438 PE=3 SV=1)

HSP 1 Score: 894.4 bits (2310), Expect = 2.1e-256
Identity = 446/497 (89.74%), Postives = 463/497 (93.16%), Query Frame = 0

Query: 1   MLWKEELVFFAQNYFD-TAVAAVLSIAILVLTSKVCWRLRSKNVGGIPGHLGLPFVGETF 60
           MLWKEELV  AQNYFD T VAAVLS+ ILVL SKVCWR RSKNVGGIPGHLGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 61  SFLSANNSTKGCYHFVRLRRLW---WFKTRLFGKVHVFVPSTEGAKAIFANDFVQFNKGY 120
           SFLSANNST+GCY FVRLRRLW   WF+TRLFGK+HVFVPS EGAKAI ANDFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 121 VKSMADAVGEKSLLCVSHENHKRIRRLLADPFSMTSLSKFVKEFDDMLSKRLKKLVKDGK 180
           VKSM DAVGEKSLLCVSHE+H+RIRRLLADPFSMTSLSKFVK+FDDMLSKRL+KLVKDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 181 SFVLLDFSMKITFDAMCNMLMSLRDESTLRQIEKDCIAVSDAMLSFPFMIPGTRYYKGIK 240
           SFVLLDFSMKITFD+MCNMLMSLRDESTLRQIEKDC AV+DAMLSFPFMIPGTRYYKGIK
Sbjct: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVTDAMLSFPFMIPGTRYYKGIK 240

Query: 241 ARNRLMERFREIISRRRRGEESPEDFLQSMLQKDSYPPDEKLDDSEIMDNLLTLIIAGQT 300
           ARNRLMER  EIISRRRRGEESPEDFLQSMLQ+DSYPPD+KLDDSEIMDNLLTLIIA QT
Sbjct: 241 ARNRLMERLGEIISRRRRGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300

Query: 301 TTAAAMMWSIKFLDENREAQERLREEQLSILRKKEDGELLSLEDLNKMYYGSKVVKETLR 360
           TTAAAMMWS+KFLDENREAQERLREEQLSILRKKEDGELL+LEDLNKM +GSKVVKETLR
Sbjct: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360

Query: 361 MSNVLVWFPRVALRDCRLEGTFSTVDKEDDSCFEIKKGWHVNIDATCIHYDPDLNKDPMQ 420
           MSNVL+WFPRVAL DCRLEG            FEIKKGWHVNIDATCIHYDPDL KDPMQ
Sbjct: 361 MSNVLLWFPRVALGDCRLEG------------FEIKKGWHVNIDATCIHYDPDLYKDPMQ 420

Query: 421 FNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGEYKWTVDDPDPCLEKK 480
           FNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTG Y+WTVDDPDPCLEKK
Sbjct: 421 FNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKK 480

Query: 481 AHIPRLRSGCPITLKTL 494
           AHIPRLRSGCPITLKTL
Sbjct: 481 AHIPRLRSGCPITLKTL 485

BLAST of Spg012884 vs. ExPASy TrEMBL
Match: A0A6J1BTT9 (abscisic acid 8'-hydroxylase 1-like OS=Momordica charantia OX=3673 GN=LOC111005488 PE=3 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 4.1e-252
Identity = 441/496 (88.91%), Postives = 456/496 (91.94%), Query Frame = 0

Query: 1   MLWKEELVFFAQNYFDTAVAAVLSIAILVLTSKVCWRLRSKNVGGIPGHLGLPFVGETFS 60
           MLWKEELVF AQNYFDTAV  VLSI IL+L SK   RLRSKNVGGIPGHLGLPFVGETFS
Sbjct: 1   MLWKEELVFLAQNYFDTAVVVVLSITILLLVSKAWRRLRSKNVGGIPGHLGLPFVGETFS 60

Query: 61  FLSANNSTKGCYHFVRLRRLW---WFKTRLFGKVHVFVPSTEGAKAIFANDFVQFNKGYV 120
           F SANNSTKGCYHFVRLRRLW   WFKTRLFGK+HVFVPS EGAKAIFA DFVQFNKGYV
Sbjct: 61  FFSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFAKDFVQFNKGYV 120

Query: 121 KSMADAVGEKSLLCVSHENHKRIRRLLADPFSMTSLSKFVKEFDDMLSKRLKKLVKDGKS 180
           KSMADAVGEKSLLCVSHENH RIRRLL+DPFSMTSLSKFVKEFDDMLS+RLKKL KDGK 
Sbjct: 121 KSMADAVGEKSLLCVSHENHGRIRRLLSDPFSMTSLSKFVKEFDDMLSERLKKLEKDGKG 180

Query: 181 FVLLDFSMKITFDAMCNMLMSLRDESTLRQIEKDCIAVSDAMLSFPFMIPGTRYYKGIKA 240
           FVLLDFSMKITFD+MCNMLMSLRDESTLRQ+EKDCIAVSDAMLSFPFMIPGTRYYKGIKA
Sbjct: 181 FVLLDFSMKITFDSMCNMLMSLRDESTLRQLEKDCIAVSDAMLSFPFMIPGTRYYKGIKA 240

Query: 241 RNRLMERFREIISRRRRGEESPEDFLQSMLQKDSYPPDEKLDDSEIMDNLLTLIIAGQTT 300
           R RLMERFREIISRRRRGEES EDFLQSMLQ+D+YPPDEKLDD+EIMDNLLTLIIAGQTT
Sbjct: 241 RTRLMERFREIISRRRRGEESQEDFLQSMLQRDAYPPDEKLDDTEIMDNLLTLIIAGQTT 300

Query: 301 TAAAMMWSIKFLDENREAQERLREEQLSILRKKEDGELLSLEDLNKMYYGSKVVKETLRM 360
           TAAAMMWSIKFLDENREAQERLREEQLSILRKK+ GE L+LEDLNKM Y  KVVKETLRM
Sbjct: 301 TAAAMMWSIKFLDENREAQERLREEQLSILRKKDYGEQLTLEDLNKMSYCLKVVKETLRM 360

Query: 361 SNVLVWFPRVALRDCRLEGTFSTVDKEDDSCFEIKKGWHVNIDATCIHYDPDLNKDPMQF 420
           SNVL+WFPRVAL DCRLEG            FEIKKGWHVNIDATCIHYDPDL KDPMQF
Sbjct: 361 SNVLLWFPRVALGDCRLEG------------FEIKKGWHVNIDATCIHYDPDLYKDPMQF 420

Query: 421 NPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGEYKWTVDDPDPCLEKKA 480
           NPSRFDEIQKPYSFIPFGSGPRTCLGINMA++TM+VFLHRMTG Y WTVDDPDPCLEKKA
Sbjct: 421 NPSRFDEIQKPYSFIPFGSGPRTCLGINMARVTMMVFLHRMTGGYMWTVDDPDPCLEKKA 480

Query: 481 HIPRLRSGCPITLKTL 494
           HIPRLRSGCPITLKTL
Sbjct: 481 HIPRLRSGCPITLKTL 484

BLAST of Spg012884 vs. ExPASy TrEMBL
Match: A0A6J1GG87 (abscisic acid 8'-hydroxylase 3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453639 PE=3 SV=1)

HSP 1 Score: 879.4 bits (2271), Expect = 7.0e-252
Identity = 447/526 (84.98%), Postives = 463/526 (88.02%), Query Frame = 0

Query: 1   MLWKEELVFFAQNYFD-TAVAAVLSIAILVLTSKVCWRLRSKNVGGIPGHLGLPFVGETF 60
           MLWKEELV  AQNYFD T VAAVLSI ILVL SKVCWR RSKNVGGIPGHLGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSIGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 61  SFLSANNSTKGCYHFVRLRRLW---WFKTRLFGKVHVFVPSTEGAKAIFANDFVQFNKGY 120
           SFLSANNST+GCY FVRLRRLW   WF+TRLFGK+HVFVPS EGAKAI ANDFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 121 VKSMADAVGEKSLLCVSHENHKRIRRLLADPFSMTSLSKFVKEFDDMLSKRLKKLVKDGK 180
           VKSM DAVGEKSLLCVSHE+H+RIRRLLADPFSMTSLSKFVK+FDDMLSKRL+KLVKDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 181 SFVLLDFSMK-----------------------------ITFDAMCNMLMSLRDESTLRQ 240
           SFVLLDFSMK                             ITFD+MCNMLMSLRDESTLRQ
Sbjct: 181 SFVLLDFSMKVIENKNIVIFLKHVCLTWQPTYLTLCDLQITFDSMCNMLMSLRDESTLRQ 240

Query: 241 IEKDCIAVSDAMLSFPFMIPGTRYYKGIKARNRLMERFREIISRRRRGEESPEDFLQSML 300
           IEKDC AVSDAMLS PFMIPGTRYYKGIKARNRLMER REIISRRR+GEESPEDFLQSML
Sbjct: 241 IEKDCSAVSDAMLSLPFMIPGTRYYKGIKARNRLMERLREIISRRRKGEESPEDFLQSML 300

Query: 301 QKDSYPPDEKLDDSEIMDNLLTLIIAGQTTTAAAMMWSIKFLDENREAQERLREEQLSIL 360
           Q+DSYPPD+KLDDSEIMDNLLTLIIA QTTTAAAMMWS+KFLDENREAQERLREEQLSIL
Sbjct: 301 QRDSYPPDQKLDDSEIMDNLLTLIIASQTTTAAAMMWSVKFLDENREAQERLREEQLSIL 360

Query: 361 RKKEDGELLSLEDLNKMYYGSKVVKETLRMSNVLVWFPRVALRDCRLEGTFSTVDKEDDS 420
           RKKEDGELL+LEDLNKM +GSKVVKETLRMSNVL+WFPRVAL DCRLEG           
Sbjct: 361 RKKEDGELLTLEDLNKMSFGSKVVKETLRMSNVLLWFPRVALGDCRLEG----------- 420

Query: 421 CFEIKKGWHVNIDATCIHYDPDLNKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMA 480
            FEIKKGWHVNIDATCIHYDPDL KDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMA
Sbjct: 421 -FEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMA 480

Query: 481 KLTMLVFLHRMTGEYKWTVDDPDPCLEKKAHIPRLRSGCPITLKTL 494
           KLTMLVFLHRMTG Y+WTVDDPDPCLEKKAHIPRLRSGCPITLKTL
Sbjct: 481 KLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPITLKTL 514

BLAST of Spg012884 vs. ExPASy TrEMBL
Match: A0A6J1IQ66 (abscisic acid 8'-hydroxylase 3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478438 PE=3 SV=1)

HSP 1 Score: 879.0 bits (2270), Expect = 9.1e-252
Identity = 446/526 (84.79%), Postives = 463/526 (88.02%), Query Frame = 0

Query: 1   MLWKEELVFFAQNYFD-TAVAAVLSIAILVLTSKVCWRLRSKNVGGIPGHLGLPFVGETF 60
           MLWKEELV  AQNYFD T VAAVLS+ ILVL SKVCWR RSKNVGGIPGHLGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 61  SFLSANNSTKGCYHFVRLRRLW---WFKTRLFGKVHVFVPSTEGAKAIFANDFVQFNKGY 120
           SFLSANNST+GCY FVRLRRLW   WF+TRLFGK+HVFVPS EGAKAI ANDFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 121 VKSMADAVGEKSLLCVSHENHKRIRRLLADPFSMTSLSKFVKEFDDMLSKRLKKLVKDGK 180
           VKSM DAVGEKSLLCVSHE+H+RIRRLLADPFSMTSLSKFVK+FDDMLSKRL+KLVKDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 181 SFVLLDFSMK-----------------------------ITFDAMCNMLMSLRDESTLRQ 240
           SFVLLDFSMK                             ITFD+MCNMLMSLRDESTLRQ
Sbjct: 181 SFVLLDFSMKVIENKNIVIFLKHVCLTWQPTYLTLCDSQITFDSMCNMLMSLRDESTLRQ 240

Query: 241 IEKDCIAVSDAMLSFPFMIPGTRYYKGIKARNRLMERFREIISRRRRGEESPEDFLQSML 300
           IEKDC AV+DAMLSFPFMIPGTRYYKGIKARNRLMER  EIISRRRRGEESPEDFLQSML
Sbjct: 241 IEKDCTAVTDAMLSFPFMIPGTRYYKGIKARNRLMERLGEIISRRRRGEESPEDFLQSML 300

Query: 301 QKDSYPPDEKLDDSEIMDNLLTLIIAGQTTTAAAMMWSIKFLDENREAQERLREEQLSIL 360
           Q+DSYPPD+KLDDSEIMDNLLTLIIA QTTTAAAMMWS+KFLDENREAQERLREEQLSIL
Sbjct: 301 QRDSYPPDQKLDDSEIMDNLLTLIIASQTTTAAAMMWSVKFLDENREAQERLREEQLSIL 360

Query: 361 RKKEDGELLSLEDLNKMYYGSKVVKETLRMSNVLVWFPRVALRDCRLEGTFSTVDKEDDS 420
           RKKEDGELL+LEDLNKM +GSKVVKETLRMSNVL+WFPRVAL DCRLEG           
Sbjct: 361 RKKEDGELLTLEDLNKMSFGSKVVKETLRMSNVLLWFPRVALGDCRLEG----------- 420

Query: 421 CFEIKKGWHVNIDATCIHYDPDLNKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMA 480
            FEIKKGWHVNIDATCIHYDPDL KDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMA
Sbjct: 421 -FEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMA 480

Query: 481 KLTMLVFLHRMTGEYKWTVDDPDPCLEKKAHIPRLRSGCPITLKTL 494
           KLTMLVFLHRMTG Y+WTVDDPDPCLEKKAHIPRLRSGCPITLKTL
Sbjct: 481 KLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPITLKTL 514

BLAST of Spg012884 vs. TAIR 10
Match: AT1G19630.1 (cytochrome P450, family 722, subfamily A, polypeptide 1 )

HSP 1 Score: 386.7 bits (992), Expect = 2.7e-107
Identity = 216/477 (45.28%), Postives = 299/477 (62.68%), Query Frame = 0

Query: 22  VLSIAILVLTS--KVCWRLRSKNVGGI-PGHLGLPFVGETFSFLSANNSTKGCYHFVRLR 81
           VL  A+L L    K+ ++ R K+  G+ PG  G P +GET  F+ + NS KG Y FVR R
Sbjct: 9   VLCAAMLTLGKFLKIMFQDRKKSTAGVPPGSDGFPVIGETLQFMLSVNSGKGFYEFVRSR 68

Query: 82  RLWW---FKTRLFGKVHVFVPSTEGAKAIFANDFVQFNKGYVKSMADAVGEKSLLCVSHE 141
           R+ +   F+T LFG+ HVF+ +TE A+ +  ND   F K Y+KS+ + VG++SLLC    
Sbjct: 69  RIRYGSCFRTSLFGETHVFLSTTESARLVLNNDSGMFTKRYIKSIGELVGDRSLLCAPQH 128

Query: 142 NHKRIRRLLADPFSMTSLSKFVKEFDDMLSKRLKKLVKDGKSFVLLDFSMKITFDAMCNM 201
           +HK +R  L + FS  S +  V+ FD+++   L      G + VLL   ++ITF AMC M
Sbjct: 129 HHKILRSRLINLFSKRSTALMVRHFDELVVDALGGWEHRG-TVVLLTDLLQITFKAMCKM 188

Query: 202 LMSLRDESTLRQIEKDCIAVSDAMLSFPFMIPGTRYYKGIKARNRLMERFREIISRRRRG 261
           L+SL  E  L  +++D   V +AML+FP  +P TR++KGI AR R+ME   +II  RR  
Sbjct: 189 LVSLEKEEELGSMQRDVGFVCEAMLAFPLNLPWTRFHKGIMARGRVMEMLEKIIRERRNE 248

Query: 262 EES----PEDFLQSMLQKDSYPPDEKLDDSEIMDNLLTLIIAGQTTTAAAMMWSIKFLDE 321
             S     EDFLQ +L  D+  P  +L D+EI DN+LT+IIAGQ TTA+A+ W +K+L E
Sbjct: 249 INSHNNHHEDFLQQLLAVDNDTP--QLTDAEIKDNILTMIIAGQDTTASALTWMVKYLGE 308

Query: 322 NREAQERLREEQLSILRKKEDGELLSLEDLNKMYYGSKVVKETLRMSNVLVWFPRVALRD 381
           N++  + L EEQ  I +K  +   L LEDL++M Y SK+VKE+LRM++V+ WFPR+ L+D
Sbjct: 309 NQKVLDILIEEQSQITKKASNKPFLELEDLSEMPYASKMVKESLRMASVVPWFPRLVLQD 368

Query: 382 CRLEGTFSTVDKEDDSCFEIKKGWHVNIDATCIHYDPDLNKDPMQFNPSRFDEIQKPYSF 441
           C +EG            ++IKKGW++NIDA  IH DP +  +P +FNP RF+E  K  SF
Sbjct: 369 CEMEG------------YKIKKGWNINIDARSIHLDPTVYSEPHKFNPLRFEEEAKANSF 428

Query: 442 IPFGSGPRTCLGINMAKLTMLVFLHRMTGEYKWTVDDPDPCLEKKAHIPRLRSGCPI 489
           + FG G RTCLG+ +AK  MLVFLHR    Y+W V D DP +EK     RL+SG PI
Sbjct: 429 LAFGMGGRTCLGLALAKAMMLVFLHRFITTYRWEVVDEDPSIEKWTLFARLKSGYPI 470

BLAST of Spg012884 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 203.0 bits (515), Expect = 5.6e-52
Identity = 142/479 (29.65%), Postives = 236/479 (49.27%), Query Frame = 0

Query: 15  FDTAVAAVLSIAILVLTSKVCWRLRSKNVGGIPGHLGLPFVGETFSFLSANNSTKGCYHF 74
           F T  AA L + +L   + V  R  S  +   PG +G P+VGETF   S + +    +  
Sbjct: 7   FLTLSAAALFLCLLRFIAGV-RRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNV---FFA 66

Query: 75  VRLRRLW-WFKTRLFGKVHVFVPSTEGAKAIFANDFVQFNKGYVKSMADAVGEKSLLCVS 134
            + RR    FKT + G   V + S E AK +       F   +  S    +G++++    
Sbjct: 67  AKQRRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQ 126

Query: 135 HENHKRIRRLLADPFSMTSLSKFVKEFDDMLSKRLKKLVKDGKSFVLLDFSMKITFDAMC 194
            + H ++R+L+   F   ++   V   + +  + L     DG            TF+   
Sbjct: 127 GDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSW--DGTQLNTYQEMKTYTFNVAL 186

Query: 195 NMLMSLRDESTLRQIEKDCIAVSD-AMLSFPFMIPGTRYYKGIKARNRLMERFREIISRR 254
             ++  +DE   R+  K C  + +    S P  +PGT ++K +KAR  L +    I+S+R
Sbjct: 187 ISILG-KDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKR 246

Query: 255 RRGEESPEDFLQSMLQKDSYPPDEKLDDSEIMDNLLTLIIAGQTTTAAAMMWSIKFLDEN 314
           R+   S  D L S ++  +      L D +I DN++ +I A + TTA+ + W +K+L +N
Sbjct: 247 RQNPSSHTDLLGSFMEDKA-----GLTDEQIADNIIGVIFAARDTTASVLTWILKYLADN 306

Query: 315 REAQERLREEQLSILRKKEDGELLSLEDLNKMYYGSKVVKETLRMSNVLVWFPRVALRDC 374
               E + EEQ++I + K++GE L+ ED  KM    +V++ETLR + +L +  R A+ D 
Sbjct: 307 PTVLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDV 366

Query: 375 RLEGTFSTVDKEDDSCFEIKKGWHVNIDATCIHYDPDLNKDPMQFNPSRFDEIQKPYSFI 434
             EG            + I KGW V      IH++ D+  DP +F+PSRF+   KP +F+
Sbjct: 367 EYEG------------YLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKPNTFM 426

Query: 435 PFGSGPRTCLGINMAKLTMLVFLHRMTGEYKWTVDDPDPCLEKKAHIPRLRSGCPITLK 492
           PFGSG  +C G  +AKL + V +H +T +Y+W++  P   ++        ++G PI L+
Sbjct: 427 PFGSGIHSCPGNELAKLEISVLIHHLTTKYRWSIVGPSDGIQ-YGPFALPQNGLPIALE 460

BLAST of Spg012884 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 199.9 bits (507), Expect = 4.7e-51
Identity = 129/421 (30.64%), Postives = 211/421 (50.12%), Query Frame = 0

Query: 47  PGHLGLPFVGETFSFLSANNSTKGCYHFVRLRRLWWFKTRLFGKVHVFVPSTEGAKAIFA 106
           PG +G P+VGETF   S + +    +   + R    FKT + G   V + S E AK +  
Sbjct: 38  PGTMGWPYVGETFQLYSQDPNV--FFQSKQKRYGSVFKTHVLGCPCVMISSPEAAKFVLV 97

Query: 107 NDFVQFNKGYVKSMADAVGEKSLLCVSHENHKRIRRLLADPFSMTSLSKFVKEFDDMLSK 166
                F   +  S    +G++++     + H ++R+L+   F   S+   V + + +   
Sbjct: 98  TKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPESIRNMVPDIESIAQD 157

Query: 167 RLKKLVKDGKSFVLLDFSMKITFDAMCNMLMSLRDESTLRQIEKDCIAVSD-AMLSFPFM 226
            L+    +G            TF+     +   +DE   R+  K C  + +    S P  
Sbjct: 158 SLRSW--EGTMINTYQEMKTYTFNVALLSIFG-KDEVLYREDLKRCYYILEKGYNSMPVN 217

Query: 227 IPGTRYYKGIKARNRLMERFREIISRRRRGEESPEDFLQSMLQKDSYPPDEKLDDSEIMD 286
           +PGT ++K +KAR  L +    I+S RR+   S  D L S +        E+L D +I D
Sbjct: 218 LPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFM-----GDKEELTDEQIAD 277

Query: 287 NLLTLIIAGQTTTAAAMMWSIKFLDENREAQERLREEQLSILRKKEDGELLSLEDLNKMY 346
           N++ +I A + TTA+ M W +K+L EN    E + EEQ++I + KE+GE L+  D  KM 
Sbjct: 278 NIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKDKEEGESLTWGDTKKMP 337

Query: 347 YGSKVVKETLRMSNVLVWFPRVALRDCRLEGTFSTVDKEDDSCFEIKKGWHVNIDATCIH 406
             S+V++ETLR++++L +  R A+ D   EG            + I KGW V      IH
Sbjct: 338 LTSRVIQETLRVASILSFTFREAVEDVEYEG------------YLIPKGWKVLPLFRNIH 397

Query: 407 YDPDLNKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGEYKWT 466
           +  D+  +P +F+PSRF+   KP +F+PFG+G  +C G  +AKL M + +H +T +Y W+
Sbjct: 398 HSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIMIHHLTTKYSWS 436

BLAST of Spg012884 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 195.3 bits (495), Expect = 1.2e-49
Identity = 136/466 (29.18%), Postives = 229/466 (49.14%), Query Frame = 0

Query: 9   FFAQNYFDTAVAAVLSIAILVLTSKVCWRL--RSKNVGGIPGHLGLPFVGETFSFLSANN 68
           FF+  Y D      L+I ++V+     W L  + + +   PG +GLP++GET    + N 
Sbjct: 11  FFSSLYADEPALITLTIVVVVVVLLFKWWLHWKEQRLRLPPGSMGLPYIGETLRLYTENP 70

Query: 69  STKGCYHFVRLRRLWW---FKTRLFGKVHVFVPSTEGAKAIFANDFVQFNKGYVKSMADA 128
           ++     F   R+  +   FKT + G   V + S E A+ +  +    F   Y  S    
Sbjct: 71  NS-----FFATRQNKYGDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERM 130

Query: 129 VGEKSLLCVSHENHKRIRRLLADPFSMTSLSKFVKEFDDMLSKRLKKLVKDGKSFVLLDF 188
           +G ++L       H  ++RL+   F  ++L   V   + ++ + L       KS   L++
Sbjct: 131 IGPEALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVLQTLSSWTSQ-KSINTLEY 190

Query: 189 SMKITFD-AMCNMLMSLRDESTLRQIEKDCIAVSDAMLSFPFMIPGTRYYKGIKARNRLM 248
             +  FD A+ +      + +T+  I+     +     S P  +PGT ++K +KAR  L 
Sbjct: 191 MKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELS 250

Query: 249 ERFREIISRRRRGEESPEDFLQSML-QKDSYPPDEKLDDSEIMDNLLTLIIAGQTTTAAA 308
           E  R++I +RR         L  +L  KD       L DS+I DN++ +I A   TTA+ 
Sbjct: 251 EELRKVIEKRRENGREEGGLLGVLLGAKDQ--KRNGLSDSQIADNIIGVIFAATDTTASV 310

Query: 309 MMWSIKFLDENREAQERLREEQLSILRK-KEDGELLSLEDLNKMYYGSKVVKETLRMSNV 368
           + W +K+L ++    + +  EQ SI +K K++   +S ED  KM   ++V++ETLR ++V
Sbjct: 311 LTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASV 370

Query: 369 LVWFPRVALRDCRLEGTFSTVDKEDDSCFEIKKGWHVNIDATCIHYDPDLNKDPMQFNPS 428
           L +  R A++D   +G            + I KGW V      IH+  +   DP +F+PS
Sbjct: 371 LSFTFREAVQDVEYDG------------YLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPS 430

Query: 429 RFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGEYKWTV 467
           RF+   KPY+++PFG+G  +C G  +AKL ML+ LH +T  ++W V
Sbjct: 431 RFEVAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEV 456

BLAST of Spg012884 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 195.3 bits (495), Expect = 1.2e-49
Identity = 136/466 (29.18%), Postives = 229/466 (49.14%), Query Frame = 0

Query: 9   FFAQNYFDTAVAAVLSIAILVLTSKVCWRL--RSKNVGGIPGHLGLPFVGETFSFLSANN 68
           FF+  Y D      L+I ++V+     W L  + + +   PG +GLP++GET    + N 
Sbjct: 11  FFSSLYADEPALITLTIVVVVVVLLFKWWLHWKEQRLRLPPGSMGLPYIGETLRLYTENP 70

Query: 69  STKGCYHFVRLRRLWW---FKTRLFGKVHVFVPSTEGAKAIFANDFVQFNKGYVKSMADA 128
           ++     F   R+  +   FKT + G   V + S E A+ +  +    F   Y  S    
Sbjct: 71  NS-----FFATRQNKYGDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERM 130

Query: 129 VGEKSLLCVSHENHKRIRRLLADPFSMTSLSKFVKEFDDMLSKRLKKLVKDGKSFVLLDF 188
           +G ++L       H  ++RL+   F  ++L   V   + ++ + L       KS   L++
Sbjct: 131 IGPEALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVLQTLSSWTSQ-KSINTLEY 190

Query: 189 SMKITFD-AMCNMLMSLRDESTLRQIEKDCIAVSDAMLSFPFMIPGTRYYKGIKARNRLM 248
             +  FD A+ +      + +T+  I+     +     S P  +PGT ++K +KAR  L 
Sbjct: 191 MKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELS 250

Query: 249 ERFREIISRRRRGEESPEDFLQSML-QKDSYPPDEKLDDSEIMDNLLTLIIAGQTTTAAA 308
           E  R++I +RR         L  +L  KD       L DS+I DN++ +I A   TTA+ 
Sbjct: 251 EELRKVIEKRRENGREEGGLLGVLLGAKDQ--KRNGLSDSQIADNIIGVIFAATDTTASV 310

Query: 309 MMWSIKFLDENREAQERLREEQLSILRK-KEDGELLSLEDLNKMYYGSKVVKETLRMSNV 368
           + W +K+L ++    + +  EQ SI +K K++   +S ED  KM   ++V++ETLR ++V
Sbjct: 311 LTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASV 370

Query: 369 LVWFPRVALRDCRLEGTFSTVDKEDDSCFEIKKGWHVNIDATCIHYDPDLNKDPMQFNPS 428
           L +  R A++D   +G            + I KGW V      IH+  +   DP +F+PS
Sbjct: 371 LSFTFREAVQDVEYDG------------YLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPS 430

Query: 429 RFDEIQKPYSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGEYKWTV 467
           RF+   KPY+++PFG+G  +C G  +AKL ML+ LH +T  ++W V
Sbjct: 431 RFEVAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEV 456

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022950585.13.3e-25689.94abscisic acid 8'-hydroxylase 3-like isoform X2 [Cucurbita moschata] >KAG7034125.... [more]
XP_022978466.14.3e-25689.74abscisic acid 8'-hydroxylase 3-like isoform X2 [Cucurbita maxima][more]
XP_023543351.15.7e-25689.94abscisic acid 8'-hydroxylase 3-like isoform X2 [Cucurbita pepo subsp. pepo][more]
KAG6603947.16.3e-25589.74Abscisic acid 8'-hydroxylase 3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038883385.14.1e-25489.92abscisic acid 8'-hydroxylase 3-like [Benincasa hispida] >XP_038883386.1 abscisic... [more]
Match NameE-valueIdentityDescription
Q50EK13.5e-5132.19Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1[more]
Q9FH767.9e-5129.65Abscisic acid 8'-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=CYP707A3 PE=1 ... [more]
Q949P16.7e-5030.64Abscisic acid 8'-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=CYP707A1 PE=1 ... [more]
Q50EK01.1e-4932.13Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1[more]
O810771.6e-4829.18Abscisic acid 8'-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=CYP707A2 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1GG591.6e-25689.94abscisic acid 8'-hydroxylase 3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1IMR32.1e-25689.74abscisic acid 8'-hydroxylase 3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1BTT94.1e-25288.91abscisic acid 8'-hydroxylase 1-like OS=Momordica charantia OX=3673 GN=LOC1110054... [more]
A0A6J1GG877.0e-25284.98abscisic acid 8'-hydroxylase 3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1IQ669.1e-25284.79abscisic acid 8'-hydroxylase 3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
AT1G19630.12.7e-10745.28cytochrome P450, family 722, subfamily A, polypeptide 1 [more]
AT5G45340.15.6e-5229.65cytochrome P450, family 707, subfamily A, polypeptide 3 [more]
AT4G19230.14.7e-5130.64cytochrome P450, family 707, subfamily A, polypeptide 1 [more]
AT2G29090.11.2e-4929.18cytochrome P450, family 707, subfamily A, polypeptide 2 [more]
AT2G29090.21.2e-4929.18cytochrome P450, family 707, subfamily A, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 312..332
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 23..456
e-value: 6.2E-14
score: 49.6
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 18..491
NoneNo IPR availablePANTHERPTHR24286:SF235CYTOCHROME P450 FAMILY PROTEINcoord: 18..491
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 400..424
score: 30.58
coord: 302..328
score: 20.48
coord: 431..441
score: 53.98
coord: 441..464
score: 21.64
coord: 282..299
score: 28.47
IPR001128Cytochrome P450PRINTSPR00385P450coord: 441..452
score: 32.3
coord: 432..441
score: 58.94
coord: 293..310
score: 30.91
coord: 349..360
score: 36.96
IPR001128Cytochrome P450PFAMPF00067p450coord: 82..473
e-value: 8.5E-61
score: 206.1
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 37..493
e-value: 1.0E-99
score: 336.3
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 36..491
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 434..443

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg012884.1Spg012884.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0016125 sterol metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen