Spg007794 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg007794
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	taxadiene 5-alpha hydroxylase
Location	scaffold4: 9102198 .. 9104023 (+)
RNA-Seq Expression	Spg007794
Synteny	Spg007794

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCAAGAATTTAGCTGCTTTTTGCTTTCATTATCGCTCTCCTTTCTAGCTGCAATCTTAATAATGTCCATTTTTCTCTTTAGAAGCAAAAATCTTCCTCCTGGGCAAATGGGTTTCCCTTGGATTGGCGAAACAATGGATTTTTACAGAGCCCAACGTAGAAACAGATTGTTCGAGGATTTTGTTGGGCCTCGAGTGGCTAAGTTTGGCAAGATTTTCAAAACAAGCTTGATGGGGTCGCCCACAGTGGTTGTTAATGGAGCTGAAGCCAACAGATTTTTCTTGTCCAATGAGTTTAAGTTGGTTGTGAGCTCGTGGCCATCAGCCTCGGTTCAGCTGATGGGCGAAGAATGCATAATGCAGAAACAAGGTGAAAGCCATCGATGTGTGCGTGGGCTTATTGGTGCAAGCCTTGCTGGTTCGAGTCTTGAGGCTATGATGCCTAAGTTGTCTAGCACAATTCGGTCTCATTTGGACAACAACTGGCTTGGCCGTGACATTATTGGTTTGTTTCACTCGGCGAAAGTCTTGACGTTTACGATAGTGTTTGAGTGTTTGATGGGGATTGGAGTTGAGCCAGATGTGTTGAGGACGTTCGAGAGAGTCTTGGAAGGAGTTTTTGCACCACCGTTCAGGTTGTTAAGCTATCATTCATTTGTTCGTATTCTAAACATTCTCTTATCACTTGTGAATCACTTGAGATGCCAATACGTTGAAGTATCAATAAGCCAAAAAATTAACTTATCGCAGGTTTCCTGGCTCAAAGTTTTCGAGAGCCAAGAAAGCACGGTTAGAGATAGAGAAAATGTTGAGGGAAGTGGTGAGAGAGAAGAGGAGGAGAATTGAAGGAAAGGTAAAAGAAGCAGAAGATGGATTATTGCTTTCAAGGATGGTAACAGCGATGATGAGAGGGGAAATTAGTGAAGTGGAAGTAATAGACAACGTGGTTTTGCTTGTTTTTGCAGCTCATGATACAACCTCGTTTGCCATTGCCATTACTTTCAAAATGTTGGCTCACCATTCAGATTGTTACTCACTTCTTCTCAAGGGTAATCTCTTTTAATATTATAATAAATTAATGAGTTGACAATCATTTTATTTTTCTATCATATAAAGTAATTTTGATTCCACCATTCTAATGATCATATCTGAGGAAGAATTGAGATATATAAAATTAAGAAGAGAAGTGTCTTGACTCAACAGAACACGCTGACATAGCGAGAAGCAAGGAACCAGGTCAGAATTTGACAATGGAAGATATAAAGAAGATGAAGTATACTTGGCAAGTAGCTCGTGAGAGTATTCGACTGTTTCCCCCTATATTCGGTTCTTTCAGGAAAGCGATTTCGGACATTGACTATGAGGGCTTCTTCATTCCCAAAGGATGGAAGGTGAAAATTTCCATCAATCGTTCTGCTTCCTCACATGAGCTGATACCTGAAGTCAAATTGTAGTCCAGAAATGACCATTGTTTTACCTCGAACCATTTTGGTTTCGTGCAGGTCTTATGGACGACGTATGGAACACACTACGAGGAAGAGTATTTTGAAGATCCATTGAGTTTCAAGCCAAGCAGATTTGAGGAACCTGTTTCCCAGTATGTATATGTTCCATTTGGAGGAGGGCCAAGAGCTTGTGCAGGCTACCAGCTAGCCAAATTGAATATCCTCATCTTTATACACTTTGTTGTAACTCGTTATGATTGGAGTTTAGTCCACCCAGATGAGCCAATCATAATGGATCCTCTTCCTTTCCCTTCTCATGGAATGCCAATAAAGATATCTCCAAAATCAGAAGTTTGTCACAATGTACAGCAAATGTAA

mRNA sequence

ATGGCTCAAGAATTTAGCTGCTTTTTGCTTTCATTATCGCTCTCCTTTCTAGCTGCAATCTTAATAATGTCCATTTTTCTCTTTAGAAGCAAAAATCTTCCTCCTGGGCAAATGGGTTTCCCTTGGATTGGCGAAACAATGGATTTTTACAGAGCCCAACGTAGAAACAGATTGTTCGAGGATTTTGTTGGGCCTCGAGTGGCTAAGTTTGGCAAGATTTTCAAAACAAGCTTGATGGGGTCGCCCACAGTGGTTGTTAATGGAGCTGAAGCCAACAGATTTTTCTTGTCCAATGAGTTTAAGTTGGTTGTGAGCTCGTGGCCATCAGCCTCGGTTCAGCTGATGGGCGAAGAATGCATAATGCAGAAACAAGGTGAAAGCCATCGATGTGTGCGTGGGCTTATTGGTGCAAGCCTTGCTGGTTCGAGTCTTGAGGCTATGATGCCTAAGTTGTCTAGCACAATTCGGTCTCATTTGGACAACAACTGGCTTGGCCGTGACATTATTGGTTTGTTTCACTCGGCGAAAGTCTTGACGTTTACGATAGTGTTTGAGTGTTTGATGGGGATTGGAGTTGAGCCAGATGTGTTGAGGACGTTCGAGAGAGTCTTGGAAGGAGTTTTTGCACCACCGTTCAGGTTTCCTGGCTCAAAGTTTTCGAGAGCCAAGAAAGCACGGTTAGAGATAGAGAAAATGTTGAGGGAAGTGGTGAGAGAGAAGAGGAGGAGAATTGAAGGAAAGGTAAAAGAAGCAGAAGATGGATTATTGCTTTCAAGGATGGTAACAGCGATGATGAGAGGGGAAATTAGTGAAGTGGAAGTAATAGACAACGTGGTTTTGCTTGTTTTTGCAGCTCATGATACAACCTCGTTTGCCATTGCCATTACTTTCAAAATGTTGGCTCACCATTCAGATTGTTACTCACTTCTTCTCAAGGAAGAGAAGTGTCTTGACTCAACAGAACACGCTGACATAGCGAGAAGCAAGGAACCAGGTCAGAATTTGACAATGGAAGATATAAAGAAGATGAAGTATACTTGGCAAGTAGCTCGTGAGAGTATTCGACTGTTTCCCCCTATATTCGGTTCTTTCAGGAAAGCGATTTCGGACATTGACTATGAGGGCTTCTTCATTCCCAAAGGATGGAAGGTCTTATGGACGACGTATGGAACACACTACGAGGAAGAGTATTTTGAAGATCCATTGAGTTTCAAGCCAAGCAGATTTGAGGAACCTGTTTCCCAGTATGTATATGTTCCATTTGGAGGAGGGCCAAGAGCTTGTGCAGGCTACCAGCTAGCCAAATTGAATATCCTCATCTTTATACACTTTGTTGTAACTCGTTATGATTGGAGTTTAGTCCACCCAGATGAGCCAATCATAATGGATCCTCTTCCTTTCCCTTCTCATGGAATGCCAATAAAGATATCTCCAAAATCAGAAGTTTGTCACAATGTACAGCAAATGTAA

Coding sequence (CDS)

ATGGCTCAAGAATTTAGCTGCTTTTTGCTTTCATTATCGCTCTCCTTTCTAGCTGCAATCTTAATAATGTCCATTTTTCTCTTTAGAAGCAAAAATCTTCCTCCTGGGCAAATGGGTTTCCCTTGGATTGGCGAAACAATGGATTTTTACAGAGCCCAACGTAGAAACAGATTGTTCGAGGATTTTGTTGGGCCTCGAGTGGCTAAGTTTGGCAAGATTTTCAAAACAAGCTTGATGGGGTCGCCCACAGTGGTTGTTAATGGAGCTGAAGCCAACAGATTTTTCTTGTCCAATGAGTTTAAGTTGGTTGTGAGCTCGTGGCCATCAGCCTCGGTTCAGCTGATGGGCGAAGAATGCATAATGCAGAAACAAGGTGAAAGCCATCGATGTGTGCGTGGGCTTATTGGTGCAAGCCTTGCTGGTTCGAGTCTTGAGGCTATGATGCCTAAGTTGTCTAGCACAATTCGGTCTCATTTGGACAACAACTGGCTTGGCCGTGACATTATTGGTTTGTTTCACTCGGCGAAAGTCTTGACGTTTACGATAGTGTTTGAGTGTTTGATGGGGATTGGAGTTGAGCCAGATGTGTTGAGGACGTTCGAGAGAGTCTTGGAAGGAGTTTTTGCACCACCGTTCAGGTTTCCTGGCTCAAAGTTTTCGAGAGCCAAGAAAGCACGGTTAGAGATAGAGAAAATGTTGAGGGAAGTGGTGAGAGAGAAGAGGAGGAGAATTGAAGGAAAGGTAAAAGAAGCAGAAGATGGATTATTGCTTTCAAGGATGGTAACAGCGATGATGAGAGGGGAAATTAGTGAAGTGGAAGTAATAGACAACGTGGTTTTGCTTGTTTTTGCAGCTCATGATACAACCTCGTTTGCCATTGCCATTACTTTCAAAATGTTGGCTCACCATTCAGATTGTTACTCACTTCTTCTCAAGGAAGAGAAGTGTCTTGACTCAACAGAACACGCTGACATAGCGAGAAGCAAGGAACCAGGTCAGAATTTGACAATGGAAGATATAAAGAAGATGAAGTATACTTGGCAAGTAGCTCGTGAGAGTATTCGACTGTTTCCCCCTATATTCGGTTCTTTCAGGAAAGCGATTTCGGACATTGACTATGAGGGCTTCTTCATTCCCAAAGGATGGAAGGTCTTATGGACGACGTATGGAACACACTACGAGGAAGAGTATTTTGAAGATCCATTGAGTTTCAAGCCAAGCAGATTTGAGGAACCTGTTTCCCAGTATGTATATGTTCCATTTGGAGGAGGGCCAAGAGCTTGTGCAGGCTACCAGCTAGCCAAATTGAATATCCTCATCTTTATACACTTTGTTGTAACTCGTTATGATTGGAGTTTAGTCCACCCAGATGAGCCAATCATAATGGATCCTCTTCCTTTCCCTTCTCATGGAATGCCAATAAAGATATCTCCAAAATCAGAAGTTTGTCACAATGTACAGCAAATGTAA

Protein sequence

MAQEFSCFLLSLSLSFLAAILIMSIFLFRSKNLPPGQMGFPWIGETMDFYRAQRRNRLFEDFVGPRVAKFGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIGASLAGSSLEAMMPKLSSTIRSHLDNNWLGRDIIGLFHSAKVLTFTIVFECLMGIGVEPDVLRTFERVLEGVFAPPFRFPGSKFSRAKKARLEIEKMLREVVREKRRRIEGKVKEAEDGLLLSRMVTAMMRGEISEVEVIDNVVLLVFAAHDTTSFAIAITFKMLAHHSDCYSLLLKEEKCLDSTEHADIARSKEPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIDYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYDWSLVHPDEPIIMDPLPFPSHGMPIKISPKSEVCHNVQQM

Homology

[Close]

BLAST of Spg007794 vs. NCBI nr
Match: XP_022147913.1 (taxadiene 5-alpha hydroxylase isoform X1 [Momordica charantia])

HSP 1 Score: 828.6 bits (2139), Expect = 2.9e-236
Identity = 421/498 (84.54%), Postives = 447/498 (89.76%), Query Frame = 0


Query: 1   MAQEFSCFLLSLSLSFLAAILIMSIFLFR--------SKNLPPGQMGFPWIGETMDFYRA 60
           MAQEF CFLL L  S LAAILIM IFLF         SK LPPGQMG PWIGETMDFYRA
Sbjct: 1   MAQEFCCFLLPL-FSILAAILIMFIFLFSKPKRSSNGSKKLPPGQMGVPWIGETMDFYRA 60

Query: 61  QRRNRLFEDFVGPRVAKFGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASV 120
           QR NRLFEDFVGPRVAK+GK+FKTSLMGSPTV+VNGAEANRFFLSNEFKLVVSSWPSASV
Sbjct: 61  QRNNRLFEDFVGPRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASV 120

Query: 121 QLMGEECIMQKQGESHRCVRGLIGASLAGSSLEAMMPKLSSTIRSHLDNNWLGRDIIGLF 180
           QLMG+ECIMQKQGE+HRC+RGLIGASLA S LEAMMPKL +TI+ HL   WLG D I LF
Sbjct: 121 QLMGKECIMQKQGETHRCLRGLIGASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALF 180

Query: 181 HSAKVLTFTIVFECLMGIGVEPDVLRTFERVLEGVFAPPFRFPGSKFSRAKKARLEIEKM 240
           HSAKVLTFTIVFECL+GI VEPDVL TFERVLEGVFAPP RFPGS+F RAKKARLEIEKM
Sbjct: 181 HSAKVLTFTIVFECLLGIKVEPDVLTTFERVLEGVFAPPIRFPGSRFLRAKKARLEIEKM 240

Query: 241 LREVVREKRRRIEGKVKEAED-GLLLSRMVTAMMRGEISEVEVIDNVVLLVFAAHDTTSF 300
           LR+VVREKRR+IEGK++E E+ G LLSRMV AM+RGEISEVEVIDNVVLLVFAAHDTTSF
Sbjct: 241 LRKVVREKRRKIEGKLEEEEEGGSLLSRMVMAMIRGEISEVEVIDNVVLLVFAAHDTTSF 300

Query: 301 AIAITFKMLAHHSDCYSLLLKEEKCLDSTEHADIARSKEPGQNLTMEDIKKMKYTWQVAR 360
           AIA+ FKMLAHH+DCY+LLL+        EH DI RSK   QNLTMEDIKKMKYTWQVAR
Sbjct: 301 AIAMAFKMLAHHTDCYTLLLR--------EHDDILRSKGEDQNLTMEDIKKMKYTWQVAR 360

Query: 361 ESIRLFPPIFGSFRKAISDIDYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEE 420
           E++RLFPPIFGSFRKAISDI+YEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEE
Sbjct: 361 ETMRLFPPIFGSFRKAISDIEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEE 420

Query: 421 PVSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYDWSLVHPDEPIIMDPLPFPSHG 480
           PVSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRY+WSL+HPDEPIIMDPLPFPSHG
Sbjct: 421 PVSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDPLPFPSHG 480

Query: 481 MPIKISPKSEVCHNVQQM 490
           MPIKISPKSEVC N+QQM
Sbjct: 481 MPIKISPKSEVCTNIQQM 489

[Close]

BLAST of Spg007794 vs. NCBI nr
Match: XP_023554746.1 (taxadiene 5-alpha hydroxylase isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 808.9 bits (2088), Expect = 2.4e-230
Identity = 414/494 (83.81%), Postives = 441/494 (89.27%), Query Frame = 0


Query: 1   MAQEFSCFLLSLSLSFLAAILIMS-IFLFR----SKNLPPGQMGFPWIGETMDFYRAQRR 60
           M QEFSCFLL+    FL+AILI+S IF+FR    SK +PPGQMG PWIGETM FYRAQR+
Sbjct: 1   MVQEFSCFLLATMFLFLSAILIISIIFVFRSIKGSKKVPPGQMGLPWIGETMAFYRAQRK 60

Query: 61  NRLFEDFVGPRVAKFGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM 120
           NRLFE+FVGPRVAK+GKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM
Sbjct: 61  NRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM 120

Query: 121 GEECIMQKQGESHRCVRGLIGASLAGSSLEAMMPKLSSTIRSHLDNNWLGRDIIGLFHSA 180
           GEECIMQKQGESHRCVRGLI ASLA SSLEAMMPKL +TI+ +LD  WLG DII L+HSA
Sbjct: 121 GEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQLYLDTKWLGHDIISLYHSA 180

Query: 181 KVLTFTIVFECLMGIGVEPDVLRTFERVLEGVFAPPFRFPGSKFSRAKKARLEIEKMLRE 240
           KVLTFTIVFECLMGI VEP +L TFERVLEGVFAPPF FPG++FSRAKKAR+EI KMLRE
Sbjct: 181 KVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLRE 240

Query: 241 VVREKRRRIEGKVKEAEDGLLLSRMVTAMMRGEISEVEVIDNVVLLVFAAHDTTSFAIAI 300
           VVREKRRRIE + +E E  LLLSRMV AM+RGEISEVEV+DNVVLLVFAAHDTTSFAIA+
Sbjct: 241 VVREKRRRIEEEPEEEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAM 300

Query: 301 TFKMLAHHSDCYSLLLKEEKCLDSTEHADIARSKEPGQNLTMEDIKKMKYTWQVARESIR 360
           TFKMLA   +CYSLLL+        EHADI RSK  GQN+TMEDIKKMKYTWQVARESIR
Sbjct: 301 TFKMLAQQPNCYSLLLQ--------EHADIVRSKGAGQNMTMEDIKKMKYTWQVARESIR 360

Query: 361 LFPPIFGSFRKAISDIDYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQ 420
           LFPPIFGSFRKAI+DI+YEGFFIPKGWKVLWTTYGTH E+EYFEDPLSFKPSRFEEPVSQ
Sbjct: 361 LFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDEYFEDPLSFKPSRFEEPVSQ 420

Query: 421 YVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYDWSLVHPDEPIIMDPLPFPSHGMPIK 480
           YVYVPFGGGPRACAGYQLAKLNILIFIH VVTRYDWSLVHPDE IIMDPLP PSHGMPIK
Sbjct: 421 YVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMDPLPIPSHGMPIK 480

Query: 481 ISPKSEVCHNVQQM 490
           ISPKSE   NVQQM
Sbjct: 481 ISPKSEARPNVQQM 486

[Close]

BLAST of Spg007794 vs. NCBI nr
Match: KAG7012036.1 (hypothetical protein SDJN02_26944, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 808.5 bits (2087), Expect = 3.1e-230
Identity = 412/494 (83.40%), Postives = 441/494 (89.27%), Query Frame = 0


Query: 1   MAQEFSCFLLSLSLSFLAAILIMSI-FLFR----SKNLPPGQMGFPWIGETMDFYRAQRR 60
           M QEFSCFLL+    FL+AILI+SI F+FR    SK +PPGQMG PWIGETM FYRAQR+
Sbjct: 1   MVQEFSCFLLATMFLFLSAILIISIVFVFRSIKGSKKVPPGQMGLPWIGETMAFYRAQRK 60

Query: 61  NRLFEDFVGPRVAKFGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM 120
           NRLFE+FVGPRVAK+GKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM
Sbjct: 61  NRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM 120

Query: 121 GEECIMQKQGESHRCVRGLIGASLAGSSLEAMMPKLSSTIRSHLDNNWLGRDIIGLFHSA 180
           GEECIMQKQGESHRC RGLI ASLA SSLEAMMPKL +TI+ +LD  W+G DII L+HSA
Sbjct: 121 GEECIMQKQGESHRCARGLIAASLASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSA 180

Query: 181 KVLTFTIVFECLMGIGVEPDVLRTFERVLEGVFAPPFRFPGSKFSRAKKARLEIEKMLRE 240
           KVLTFTIVFECLMGI VEP +L TFERVLEGVFAPPF FPG++FSRAKKAR+EI KMLRE
Sbjct: 181 KVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLRE 240

Query: 241 VVREKRRRIEGKVKEAEDGLLLSRMVTAMMRGEISEVEVIDNVVLLVFAAHDTTSFAIAI 300
           VVREKRRRIE + +  E  LLLSRMV AM+RGEISEVEV+DNVVLLVFAAHDTTSFAIA+
Sbjct: 241 VVREKRRRIEEEPEGEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAV 300

Query: 301 TFKMLAHHSDCYSLLLKEEKCLDSTEHADIARSKEPGQNLTMEDIKKMKYTWQVARESIR 360
           TFKMLA   +CYSLLL+        EHADI RSK PGQNLTMEDIKKMKYTWQVARESIR
Sbjct: 301 TFKMLAQQPNCYSLLLQ--------EHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIR 360

Query: 361 LFPPIFGSFRKAISDIDYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQ 420
           LFPPIFGSFRKAI+DI+YEGFFIPKGWKVLWTTYGTH ++EYFEDPLSFKPSRFEEPVSQ
Sbjct: 361 LFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLKDEYFEDPLSFKPSRFEEPVSQ 420

Query: 421 YVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYDWSLVHPDEPIIMDPLPFPSHGMPIK 480
           YVYVPFGGGPRACAGYQLAKLNILIFIH VVTRYDWSL+HPDEPIIMDPLP PSHGMPIK
Sbjct: 421 YVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLIHPDEPIIMDPLPIPSHGMPIK 480

Query: 481 ISPKSEVCHNVQQM 490
           ISPKSE   NVQQM
Sbjct: 481 ISPKSESHPNVQQM 486

[Close]

BLAST of Spg007794 vs. NCBI nr
Match: XP_022952282.1 (taxadiene 5-alpha hydroxylase isoform X2 [Cucurbita moschata])

HSP 1 Score: 805.8 bits (2080), Expect = 2.0e-229
Identity = 413/494 (83.60%), Postives = 440/494 (89.07%), Query Frame = 0


Query: 1   MAQEFSCFLLSLSLSFLAAILIMS-IFLFR----SKNLPPGQMGFPWIGETMDFYRAQRR 60
           M QEFSCFLL+    FL+AILI+S IF+FR    SK +PPGQMG PWIGETM FYRAQR+
Sbjct: 1   MVQEFSCFLLATMFLFLSAILIISIIFVFRSIKGSKKVPPGQMGLPWIGETMVFYRAQRK 60

Query: 61  NRLFEDFVGPRVAKFGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM 120
           NRLFE+FVGPRVAK+GKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM
Sbjct: 61  NRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM 120

Query: 121 GEECIMQKQGESHRCVRGLIGASLAGSSLEAMMPKLSSTIRSHLDNNWLGRDIIGLFHSA 180
           GEECIMQKQGESHRCVRGLI ASLA SSLEAMMPKL +TI+ +LD  WLG DII L+HSA
Sbjct: 121 GEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQLYLDTKWLGHDIISLYHSA 180

Query: 181 KVLTFTIVFECLMGIGVEPDVLRTFERVLEGVFAPPFRFPGSKFSRAKKARLEIEKMLRE 240
           KVLTFTIVFECLMGI VEP +L TFERVLEGVFAPPF FPG++FSRAKKAR+EI KMLRE
Sbjct: 181 KVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLRE 240

Query: 241 VVREKRRRIEGKVKEAEDGLLLSRMVTAMMRGEISEVEVIDNVVLLVFAAHDTTSFAIAI 300
           VVREKRRRIE +  E E  LLLSRMV AM+RGEISEVEV+DNVVLLVFAAHDTTSFAIA+
Sbjct: 241 VVREKRRRIEEEPVEEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAM 300

Query: 301 TFKMLAHHSDCYSLLLKEEKCLDSTEHADIARSKEPGQNLTMEDIKKMKYTWQVARESIR 360
           TFKMLA   +CYSLLL+        EHADI RSK  GQN+TMEDIKKMKYTWQVARESIR
Sbjct: 301 TFKMLAQQPNCYSLLLQ--------EHADIVRSKGAGQNMTMEDIKKMKYTWQVARESIR 360

Query: 361 LFPPIFGSFRKAISDIDYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQ 420
           LFPPIFGSFRKAI+DI+YEGFFIPKGWKVLWTTYGTH E++YFEDPLSFKPSRFEEPVSQ
Sbjct: 361 LFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDKYFEDPLSFKPSRFEEPVSQ 420

Query: 421 YVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYDWSLVHPDEPIIMDPLPFPSHGMPIK 480
           YVYVPFGGGPRACAGYQLAKLNILIFIH VVTRYDWSLVHPDE IIMDPLP PSHGMPIK
Sbjct: 421 YVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMDPLPIPSHGMPIK 480

Query: 481 ISPKSEVCHNVQQM 490
           ISPKSE   NVQQM
Sbjct: 481 ISPKSEARPNVQQM 486

[Close]

BLAST of Spg007794 vs. NCBI nr
Match: XP_022147914.1 (taxadiene 5-alpha hydroxylase isoform X2 [Momordica charantia] >XP_022147915.1 taxadiene 5-alpha hydroxylase isoform X2 [Momordica charantia] >XP_022147916.1 taxadiene 5-alpha hydroxylase isoform X2 [Momordica charantia] >XP_022147917.1 taxadiene 5-alpha hydroxylase isoform X2 [Momordica charantia])

HSP 1 Score: 791.6 bits (2043), Expect = 3.9e-225
Identity = 392/453 (86.53%), Postives = 418/453 (92.27%), Query Frame = 0


Query: 38  MGFPWIGETMDFYRAQRRNRLFEDFVGPRVAKFGKIFKTSLMGSPTVVVNGAEANRFFLS 97
           MG PWIGETMDFYRAQR NRLFEDFVGPRVAK+GK+FKTSLMGSPTV+VNGAEANRFFLS
Sbjct: 1   MGVPWIGETMDFYRAQRNNRLFEDFVGPRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLS 60

Query: 98  NEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIGASLAGSSLEAMMPKLSSTIRS 157
           NEFKLVVSSWPSASVQLMG+ECIMQKQGE+HRC+RGLIGASLA S LEAMMPKL +TI+ 
Sbjct: 61  NEFKLVVSSWPSASVQLMGKECIMQKQGETHRCLRGLIGASLASSCLEAMMPKLCTTIQF 120

Query: 158 HLDNNWLGRDIIGLFHSAKVLTFTIVFECLMGIGVEPDVLRTFERVLEGVFAPPFRFPGS 217
           HL   WLG D I LFHSAKVLTFTIVFECL+GI VEPDVL TFERVLEGVFAPP RFPGS
Sbjct: 121 HLATTWLGHDTIALFHSAKVLTFTIVFECLLGIKVEPDVLTTFERVLEGVFAPPIRFPGS 180

Query: 218 KFSRAKKARLEIEKMLREVVREKRRRIEGKVKEAED-GLLLSRMVTAMMRGEISEVEVID 277
           +F RAKKARLEIEKMLR+VVREKRR+IEGK++E E+ G LLSRMV AM+RGEISEVEVID
Sbjct: 181 RFLRAKKARLEIEKMLRKVVREKRRKIEGKLEEEEEGGSLLSRMVMAMIRGEISEVEVID 240

Query: 278 NVVLLVFAAHDTTSFAIAITFKMLAHHSDCYSLLLKEEKCLDSTEHADIARSKEPGQNLT 337
           NVVLLVFAAHDTTSFAIA+ FKMLAHH+DCY+LLL+        EH DI RSK   QNLT
Sbjct: 241 NVVLLVFAAHDTTSFAIAMAFKMLAHHTDCYTLLLR--------EHDDILRSKGEDQNLT 300

Query: 338 MEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIDYEGFFIPKGWKVLWTTYGTHYEEE 397
           MEDIKKMKYTWQVARE++RLFPPIFGSFRKAISDI+YEGFFIPKGWKVLWTTYGTHYEEE
Sbjct: 301 MEDIKKMKYTWQVARETMRLFPPIFGSFRKAISDIEYEGFFIPKGWKVLWTTYGTHYEEE 360

Query: 398 YFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYDWSLVHP 457
           YFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRY+WSL+HP
Sbjct: 361 YFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYNWSLIHP 420

Query: 458 DEPIIMDPLPFPSHGMPIKISPKSEVCHNVQQM 490
           DEPIIMDPLPFPSHGMPIKISPKSEVC N+QQM
Sbjct: 421 DEPIIMDPLPFPSHGMPIKISPKSEVCTNIQQM 445

[Close]

BLAST of Spg007794 vs. ExPASy Swiss-Prot
Match: Q6WG30 (Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2)

HSP 1 Score: 357.1 bits (915), Expect = 3.2e-97
Identity = 196/483 (40.58%), Postives = 301/483 (62.32%), Query Frame = 0


Query: 11  SLSLSFLAAILIMSIFLFRSK-----NLPPGQMGFPWIGETMDFYRAQRRNRLFEDFVGP 70
           S++LS +A IL++ + LFRSK      LPPG++G P+IGE+  F RA R N L E F   
Sbjct: 26  SIALSAIAGILLL-LLLFRSKRHSSLKLPPGKLGIPFIGESFIFLRALRSNSL-EQFFDE 85

Query: 71  RVAKFGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQG 130
           RV KFG +FKTSL+G PTVV+ G   NR  LSNE KLV  SWP+  ++LMGE  +  ++G
Sbjct: 86  RVKKFGLVFKTSLIGHPTVVLCGPAGNRLILSNEEKLVQMSWPAQFMKLMGENSVATRRG 145

Query: 131 ESHRCVRGLIGASLAGSSLEAMMPKLSSTIRSHLDNNWLGRDIIGLFHSAKVLTFTIVFE 190
           E H  +R  +       +L++ + K+++ I+SH++  W G+D + +    + L F I   
Sbjct: 146 EDHIVMRSALAGFFGPGALQSYIGKMNTEIQSHINEKWKGKDEVNVLPLVRELVFNISAI 205

Query: 191 CLMGI--GVEPDVL-RTFERVLEGVFAPPFRFPGSKFSRAKKARLEIEKMLREVVREKRR 250
               I    E D L +  E +L G FA P   PG  F RA + R ++ K++  ++++++ 
Sbjct: 206 LFFNIYDKQEQDRLHKLLETILVGSFALPIDLPGFGFHRALQGRAKLNKIMLSLIKKRKE 265

Query: 251 RIEGKVKEAEDGLLLSRMVTAMMRG-EISEVEVIDNVVLLVFAAHDTTSFAIAITFKMLA 310
            ++     A   LL   +     +G  ++  E++DN   L+ A++DTT+  +A+ FK+L+
Sbjct: 266 DLQSGSATATQDLLSVLLTFRDDKGTPLTNDEILDNFSSLLHASYDTTTSPMALIFKLLS 325

Query: 311 HHSDCYSLLLKEEKCLDSTEHADIARSKEPGQNLTMEDIKKMKYTWQVARESIRLFPPIF 370
            + +CY  +++E+         +I  +KE G+ +T +D+K MKYTWQVA+E++R+FPP+F
Sbjct: 326 SNPECYQKVVQEQ--------LEILSNKEEGEEITWKDLKAMKYTWQVAQETLRMFPPVF 385

Query: 371 GSFRKAISDIDYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEP---VSQYVY 430
           G+FRKAI+DI Y+G+ IPKGWK+LWTTY TH ++ YF +P  F PSRF++    V+ Y +
Sbjct: 386 GTFRKAITDIQYDGYTIPKGWKLLWTTYSTHPKDLYFNEPEKFMPSRFDQEGKHVAPYTF 445

Query: 431 VPFGGGPRACAGYQLAKLNILIFI-HFVVTRYDWSLVHPDEPIIMDPL-PFPSHGMPIKI 480
           +PFGGG R+C G++ +K+ IL+F+ HFV T   ++ V PDE I  DPL P PS G  IK+
Sbjct: 446 LPFGGGQRSCVGWEFSKMEILLFVHHFVKTFSSYTPVDPDEKISGDPLPPLPSKGFSIKL 498

[Close]

BLAST of Spg007794 vs. ExPASy Swiss-Prot
Match: Q50EK0 (Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 1.2e-91
Identity = 186/480 (38.75%), Postives = 280/480 (58.33%), Query Frame = 0


Query: 16  FLAAILIMSIFLFRSKN--------LPPGQMGFPWIGETMDFYRAQRRNRLFEDFVGPRV 75
           FLA+ + ++ F +   N        +PPG  G+P IGET++F   QR+     DF   R 
Sbjct: 26  FLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNP-RDFFDSRT 85

Query: 76  AKFGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGES 135
            K+G +F TSL+G PTVV    E NRF  SNE KLVV+SWPS SV  +    ++   G+ 
Sbjct: 86  QKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENKLVVNSWPS-SVGNLFRSSLITTVGDD 145

Query: 136 HRCVRGLIGASLAGSSLEAMMPKLSSTIRSHLDNNWLGRDIIGLFHSAKVLTFTIVFECL 195
            + +R ++   L   +L   + ++ S  + HL  +W+G+D +      K  TF++  +  
Sbjct: 146 AKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVTALPLLKRYTFSLACDLF 205

Query: 196 MGIGVEPDVLRT---FERVLEGVFAPPFRFPGSKFSRAKKARLEIEKMLREVVREKRRRI 255
             I  + D+ R    F   ++GV   P   PG+++++AK A   I + L  ++ E++  +
Sbjct: 206 ASINNKDDLGRLWLHFMVFVKGVMQIPIDLPGTRYNKAKHAANAIRQQLGSIINERKIGL 265

Query: 256 EGKVKEAEDGLLLSRMVTAMMRGE-ISEVEVIDNVVLLVFAAHDTTSFAIAITFKMLAHH 315
           E      E  LL   +     +GE +++ E+ DN++LL++A HDT+S  + +  K LA +
Sbjct: 266 EAGNASPEQDLLSFLLSNVDEQGESLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFLAEN 325

Query: 316 SDCYSLLLKEEKCLDSTEHADIARSKEPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGS 375
             CY  +L+E+         DIA SKE GQ L  ED+++MKY+W+VA+E++RLFP   GS
Sbjct: 326 PHCYEEVLREQ--------LDIAGSKEAGQLLEWEDLQRMKYSWRVAQEALRLFPAAQGS 385

Query: 376 FRKAISDIDYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFE-EPVSQYVYVPFG 435
           FRKAI +  Y+GF IPKGWK+ WT   TH + EYF +P +F PSRFE E    Y +VPFG
Sbjct: 386 FRKAIKEFIYDGFTIPKGWKMYWTVNSTHRKSEYFSNPETFDPSRFEGEGPPPYTFVPFG 445

Query: 436 GGPRACAGYQLAKLNILIFIHFVVTRYDWSLVHPDEPIIMDPLPFPSHGMPIKISPKSEV 483
           GGPR C G + A+L IL+F+H +V    W+LV+P E +I+DP+P P +G+PIK+ P   V
Sbjct: 446 GGPRMCPGNEFARLEILVFLHNIVKNCKWNLVNPGEKVIVDPMPAPVNGLPIKLVPHDSV 495

[Close]

BLAST of Spg007794 vs. ExPASy Swiss-Prot
Match: Q50EK1 (Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 4.5e-91
Identity = 183/476 (38.45%), Postives = 280/476 (58.82%), Query Frame = 0


Query: 16  FLAAILIMSIFLFRSKN--------LPPGQMGFPWIGETMDFYRAQRRNRLFEDFVGPRV 75
           FLA+ + ++ F +   N        +PPG  G+P IGET++F   QR+     DF   R 
Sbjct: 26  FLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNP-RDFFDSRT 85

Query: 76  AKFGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGES 135
            K+G +F TSL+G PTVV    E NRF  SNE KLVV+SWPS SV  +    ++   G+ 
Sbjct: 86  QKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENKLVVNSWPS-SVGNLFRSSLITTVGDD 145

Query: 136 HRCVRGLIGASLAGSSLEAMMPKLSSTIRSHLDNNWLGRDIIGLFHSAKVLTFTIVFECL 195
            + +R ++   L   +L   + ++ S  + HL  +W+G+D +      K  TF++  +  
Sbjct: 146 AKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVMALPLLKRYTFSLACDLF 205

Query: 196 MGIGVEPDVLRT---FERVLEGVFAPPFRFPGSKFSRAKKARLEIEKMLREVVREKRRRI 255
             I  + D+ R    F   ++GV   P   PG+++++ K A   I + L  ++ E++  +
Sbjct: 206 ASINTKDDLDRLWLHFMVFVKGVMQIPIDLPGTRYNKTKHAANAIRQQLGSIINERKIAL 265

Query: 256 EGKVKEAEDGLLLSRMVTAMMRGE-ISEVEVIDNVVLLVFAAHDTTSFAIAITFKMLAHH 315
           E      E  LL   +     +GE +++ E+ DN++LL++A HDT+S  + +  K LA +
Sbjct: 266 EAGNASPEQDLLSFLLSNVDEQGESLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFLAEN 325

Query: 316 SDCYSLLLKEEKCLDSTEHADIARSKEPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGS 375
             CY  +L+E+         +IA SKE GQ L  ED+++MKY+W+VA+E++RLFP + GS
Sbjct: 326 PHCYEEVLREQ--------LNIAGSKEEGQLLEWEDLQRMKYSWRVAQEALRLFPAVQGS 385

Query: 376 FRKAISDIDYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFE-EPVSQYVYVPFG 435
           FRKAI +  Y+GF IPKGWK+ WT   TH + EYF +P  F PSRFE E    Y +VPFG
Sbjct: 386 FRKAIKEFIYDGFTIPKGWKLHWTVNSTHQKSEYFSNPEKFDPSRFEGEGPPPYTFVPFG 445

Query: 436 GGPRACAGYQLAKLNILIFIHFVVTRYDWSLVHPDEPIIMDPLPFPSHGMPIKISP 479
           GGPR C G + A++ ILIF+H +V  ++W+LV+P E +I+DP+P P +G+PIK+ P
Sbjct: 446 GGPRMCPGNEFARMEILIFLHNIVKNFNWNLVNPLEKVIVDPMPAPVNGLPIKLVP 491

[Close]

BLAST of Spg007794 vs. ExPASy Swiss-Prot
Match: Q8W4T9 (Taxane 13-alpha-hydroxylase OS=Taxus cuspidata OX=99806 GN=CYP725A2 PE=1 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.7e-90
Identity = 186/491 (37.88%), Postives = 290/491 (59.06%), Query Frame = 0


Query: 4   EFSCFLLSLSLSFLAAILIMSIFLFRSK-----NLPPGQMGFPWIGETMDFYRAQRRNRL 63
           E S  +L ++L+ +A I    I  FRSK      LPPG +GFP +GET+ F R+   +  
Sbjct: 8   EVSPSILFVTLAVMAGI----ILFFRSKRHSSVKLPPGNLGFPLVGETLQFVRSLGSSTP 67

Query: 64  FEDFVGPRVAKFGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEE 123
            + F+  R++KFG +FKTS++G PTVV+ G   NR  LSNE KLV  SWPS+ ++L+GE+
Sbjct: 68  -QQFIEERMSKFGDVFKTSIIGHPTVVLCGPAGNRLVLSNENKLVQMSWPSSMMKLIGED 127

Query: 124 CIMQKQGESHRCVRGLIGASLAGSSLEAMMPKLSSTIRSHLDNNWLGRDIIGLFHSAKVL 183
           C+  K GE HR VR  +   L   +L+    K+SS I+ H++  W G+D   +    K L
Sbjct: 128 CLGGKTGEQHRIVRAALTRFLGPQALQNHFAKMSSGIQRHINEKWKGKDEATVLPLVKDL 187

Query: 184 TFTIVFECLMGI---GVEPDVLRTFERVLEGVFAPPFRFPGSKFSRAKKARLEIEKMLRE 243
            F++      GI    ++  +    E +L G F+ P   PG  + +A +AR  +  ++  
Sbjct: 188 VFSVASRLFFGITEEHLQEQLHNLLEVILVGSFSVPLNIPGFSYHKAIQARATLADIMTH 247

Query: 244 VVREKRRRIE-GKVKEAEDGLLLSRMVTAMMRGEISEVEVIDNVVLLVFAAHDTTSFAIA 303
           ++ ++R  +  G   E +D L +    T      +++ E++DN  +L+  ++D+T+  + 
Sbjct: 248 LIEKRRNELRAGTASENQDLLSVLLTFTDERGNSLADKEILDNFSMLLHGSYDSTNSPLT 307

Query: 304 ITFKMLAHHSDCYSLLLKEEKCLDSTEHADIARSKEPGQNLTMEDIKKMKYTWQVARESI 363
           +  K+LA H + Y  + +E+  + ST        K  G+ +  +D+K+MKY+WQV +E++
Sbjct: 308 MLIKVLASHPESYEKVAQEQFGILST--------KMEGEEIAWKDLKEMKYSWQVVQETL 367

Query: 364 RLFPPIFGSFRKAISDIDYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEP-- 423
           R++PPIFG+FRKAI+DI Y G+ IPKGWK+LWTTY T  +EEYF+D   FKPSRFEE   
Sbjct: 368 RMYPPIFGTFRKAITDIHYNGYTIPKGWKLLWTTYSTQTKEEYFKDADQFKPSRFEEEGK 427

Query: 424 -VSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYD-WSLVHPDEPIIMDPL-PFPS 481
            V+ Y Y+PFGGG R C G++ AK+  L+F+H  V  +     + P+E +   PL P P 
Sbjct: 428 HVTPYTYLPFGGGMRVCPGWEFAKMETLLFLHHFVKAFSGLKAIDPNEKLSGKPLPPLPV 485

[Close]

BLAST of Spg007794 vs. ExPASy Swiss-Prot
Match: Q84KI1 (Taxoid 14-beta-hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 3.3e-89
Identity = 190/499 (38.08%), Postives = 292/499 (58.52%), Query Frame = 0


Query: 7   CF--LLSLSLSFLAAILIMSIFLFRSK-----NLPPGQMGFPWIGETMDFYRAQRRNRLF 66
           CF  +L + LS +A I++  +   RSK      LPPG++G+P+IGE++ F +A R N + 
Sbjct: 17  CFPAILFIVLSAVAGIVLPLLLFLRSKRRSSVGLPPGKLGYPFIGESLLFLKALRSNTV- 76

Query: 67  EDFVGPRVAKFGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEEC 126
           E F+  RV  FG +FKTSL+G PTVV+ G   NR  L+NE KLV  SWP +S++LMGE+ 
Sbjct: 77  EQFLDERVKNFGNVFKTSLIGHPTVVLCGPAGNRLILANEEKLVQMSWPKSSMKLMGEKS 136

Query: 127 IMQKQGESHRCVRGLIGASLAGSSLEAMMPKLSSTIRSHLDNNWLGRDIIGLFHSAKVLT 186
           I  K+GE H  +R  +    +  +L+  + ++S TI +H++  W G D + +      L 
Sbjct: 137 ITAKRGEGHMIIRSALQGFFSPGALQKYIGQMSKTIENHINEKWKGNDQVSVVALVGDLV 196

Query: 187 FTIVFECLMGIGVEPDVLRTFER---VLEGVFAPPFRFPGSKFSRAKKARLEIEKMLREV 246
           F I       I  + +  R FE    +  GV A P   PG  + RA +AR ++  +L  +
Sbjct: 197 FDISACLFFNINEKHERERLFELLEIIAVGVLAVPVDLPGFAYHRALQARSKLNAILSGL 256

Query: 247 VREKRRRIEGKVKEAEDGLLLSRMVTAMMRGE-ISEVEVIDNVVLLVFAAHDTTSFAIAI 306
           + +++  +   +  +   LL   +     RG   S+ E++DN   L+  ++DTT  A+A 
Sbjct: 257 IEKRKMDLSSGLATSNQDLLSVFLTFKDDRGNPCSDEEILDNFSGLLHGSYDTTVSAMAC 316

Query: 307 TFKMLAHHSDCYSLLLKEEKCLDSTEHADIARSKEPGQNLTMEDIKKMKYTWQVARESIR 366
            FK+L+ + +CY  +++E+          I  +K  G  +T +D+K MKYTWQV +E++R
Sbjct: 317 VFKLLSSNPECYEKVVQEQ--------LGILSNKLEGDEITWKDVKSMKYTWQVVQETLR 376

Query: 367 LFPPIFGSFRKAISDIDYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEP--- 426
           L+P IFGSFR+AI+DI Y G+ IPKGWK+LWT Y TH +E YF +P  F PSRF++    
Sbjct: 377 LYPSIFGSFRQAITDIHYNGYIIPKGWKLLWTPYTTHPKEMYFSEPEKFLPSRFDQEGKL 436

Query: 427 VSQYVYVPFGGGPRACAGYQLAKLNILIFI-HFVVTRYDWSLVHPDEPIIMDPL-PFPSH 486
           V+ Y ++PFGGG R+C G++ +K+ IL+ + HFV T   ++ V P E I  D L P PS+
Sbjct: 437 VAPYTFLPFGGGQRSCPGWEFSKMEILLSVHHFVKTFSTFTPVDPAEIIARDSLCPLPSN 496

Query: 487 GMPIKISPKSEVCHNVQQM 490
           G  +K+ P+S   H   Q+
Sbjct: 497 GFSVKLFPRSYSLHTGNQV 506

[Close]

BLAST of Spg007794 vs. ExPASy TrEMBL
Match: A0A6J1D2L6 (taxadiene 5-alpha hydroxylase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016729 PE=3 SV=1)

HSP 1 Score: 828.6 bits (2139), Expect = 1.4e-236
Identity = 421/498 (84.54%), Postives = 447/498 (89.76%), Query Frame = 0


Query: 1   MAQEFSCFLLSLSLSFLAAILIMSIFLFR--------SKNLPPGQMGFPWIGETMDFYRA 60
           MAQEF CFLL L  S LAAILIM IFLF         SK LPPGQMG PWIGETMDFYRA
Sbjct: 1   MAQEFCCFLLPL-FSILAAILIMFIFLFSKPKRSSNGSKKLPPGQMGVPWIGETMDFYRA 60

Query: 61  QRRNRLFEDFVGPRVAKFGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASV 120
           QR NRLFEDFVGPRVAK+GK+FKTSLMGSPTV+VNGAEANRFFLSNEFKLVVSSWPSASV
Sbjct: 61  QRNNRLFEDFVGPRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASV 120

Query: 121 QLMGEECIMQKQGESHRCVRGLIGASLAGSSLEAMMPKLSSTIRSHLDNNWLGRDIIGLF 180
           QLMG+ECIMQKQGE+HRC+RGLIGASLA S LEAMMPKL +TI+ HL   WLG D I LF
Sbjct: 121 QLMGKECIMQKQGETHRCLRGLIGASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALF 180

Query: 181 HSAKVLTFTIVFECLMGIGVEPDVLRTFERVLEGVFAPPFRFPGSKFSRAKKARLEIEKM 240
           HSAKVLTFTIVFECL+GI VEPDVL TFERVLEGVFAPP RFPGS+F RAKKARLEIEKM
Sbjct: 181 HSAKVLTFTIVFECLLGIKVEPDVLTTFERVLEGVFAPPIRFPGSRFLRAKKARLEIEKM 240

Query: 241 LREVVREKRRRIEGKVKEAED-GLLLSRMVTAMMRGEISEVEVIDNVVLLVFAAHDTTSF 300
           LR+VVREKRR+IEGK++E E+ G LLSRMV AM+RGEISEVEVIDNVVLLVFAAHDTTSF
Sbjct: 241 LRKVVREKRRKIEGKLEEEEEGGSLLSRMVMAMIRGEISEVEVIDNVVLLVFAAHDTTSF 300

Query: 301 AIAITFKMLAHHSDCYSLLLKEEKCLDSTEHADIARSKEPGQNLTMEDIKKMKYTWQVAR 360
           AIA+ FKMLAHH+DCY+LLL+        EH DI RSK   QNLTMEDIKKMKYTWQVAR
Sbjct: 301 AIAMAFKMLAHHTDCYTLLLR--------EHDDILRSKGEDQNLTMEDIKKMKYTWQVAR 360

Query: 361 ESIRLFPPIFGSFRKAISDIDYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEE 420
           E++RLFPPIFGSFRKAISDI+YEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEE
Sbjct: 361 ETMRLFPPIFGSFRKAISDIEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEE 420

Query: 421 PVSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYDWSLVHPDEPIIMDPLPFPSHG 480
           PVSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRY+WSL+HPDEPIIMDPLPFPSHG
Sbjct: 421 PVSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDPLPFPSHG 480

Query: 481 MPIKISPKSEVCHNVQQM 490
           MPIKISPKSEVC N+QQM
Sbjct: 481 MPIKISPKSEVCTNIQQM 489

[Close]

BLAST of Spg007794 vs. ExPASy TrEMBL
Match: A0A6J1GJY9 (taxadiene 5-alpha hydroxylase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111455006 PE=3 SV=1)

HSP 1 Score: 805.8 bits (2080), Expect = 9.7e-230
Identity = 413/494 (83.60%), Postives = 440/494 (89.07%), Query Frame = 0


Query: 1   MAQEFSCFLLSLSLSFLAAILIMS-IFLFR----SKNLPPGQMGFPWIGETMDFYRAQRR 60
           M QEFSCFLL+    FL+AILI+S IF+FR    SK +PPGQMG PWIGETM FYRAQR+
Sbjct: 1   MVQEFSCFLLATMFLFLSAILIISIIFVFRSIKGSKKVPPGQMGLPWIGETMVFYRAQRK 60

Query: 61  NRLFEDFVGPRVAKFGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM 120
           NRLFE+FVGPRVAK+GKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM
Sbjct: 61  NRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM 120

Query: 121 GEECIMQKQGESHRCVRGLIGASLAGSSLEAMMPKLSSTIRSHLDNNWLGRDIIGLFHSA 180
           GEECIMQKQGESHRCVRGLI ASLA SSLEAMMPKL +TI+ +LD  WLG DII L+HSA
Sbjct: 121 GEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQLYLDTKWLGHDIISLYHSA 180

Query: 181 KVLTFTIVFECLMGIGVEPDVLRTFERVLEGVFAPPFRFPGSKFSRAKKARLEIEKMLRE 240
           KVLTFTIVFECLMGI VEP +L TFERVLEGVFAPPF FPG++FSRAKKAR+EI KMLRE
Sbjct: 181 KVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLRE 240

Query: 241 VVREKRRRIEGKVKEAEDGLLLSRMVTAMMRGEISEVEVIDNVVLLVFAAHDTTSFAIAI 300
           VVREKRRRIE +  E E  LLLSRMV AM+RGEISEVEV+DNVVLLVFAAHDTTSFAIA+
Sbjct: 241 VVREKRRRIEEEPVEEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAM 300

Query: 301 TFKMLAHHSDCYSLLLKEEKCLDSTEHADIARSKEPGQNLTMEDIKKMKYTWQVARESIR 360
           TFKMLA   +CYSLLL+        EHADI RSK  GQN+TMEDIKKMKYTWQVARESIR
Sbjct: 301 TFKMLAQQPNCYSLLLQ--------EHADIVRSKGAGQNMTMEDIKKMKYTWQVARESIR 360

Query: 361 LFPPIFGSFRKAISDIDYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQ 420
           LFPPIFGSFRKAI+DI+YEGFFIPKGWKVLWTTYGTH E++YFEDPLSFKPSRFEEPVSQ
Sbjct: 361 LFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDKYFEDPLSFKPSRFEEPVSQ 420

Query: 421 YVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYDWSLVHPDEPIIMDPLPFPSHGMPIK 480
           YVYVPFGGGPRACAGYQLAKLNILIFIH VVTRYDWSLVHPDE IIMDPLP PSHGMPIK
Sbjct: 421 YVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMDPLPIPSHGMPIK 480

Query: 481 ISPKSEVCHNVQQM 490
           ISPKSE   NVQQM
Sbjct: 481 ISPKSEARPNVQQM 486

[Close]

BLAST of Spg007794 vs. ExPASy TrEMBL
Match: A0A6J1D1G7 (taxadiene 5-alpha hydroxylase isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016729 PE=3 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 1.9e-225
Identity = 392/453 (86.53%), Postives = 418/453 (92.27%), Query Frame = 0


Query: 38  MGFPWIGETMDFYRAQRRNRLFEDFVGPRVAKFGKIFKTSLMGSPTVVVNGAEANRFFLS 97
           MG PWIGETMDFYRAQR NRLFEDFVGPRVAK+GK+FKTSLMGSPTV+VNGAEANRFFLS
Sbjct: 1   MGVPWIGETMDFYRAQRNNRLFEDFVGPRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLS 60

Query: 98  NEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIGASLAGSSLEAMMPKLSSTIRS 157
           NEFKLVVSSWPSASVQLMG+ECIMQKQGE+HRC+RGLIGASLA S LEAMMPKL +TI+ 
Sbjct: 61  NEFKLVVSSWPSASVQLMGKECIMQKQGETHRCLRGLIGASLASSCLEAMMPKLCTTIQF 120

Query: 158 HLDNNWLGRDIIGLFHSAKVLTFTIVFECLMGIGVEPDVLRTFERVLEGVFAPPFRFPGS 217
           HL   WLG D I LFHSAKVLTFTIVFECL+GI VEPDVL TFERVLEGVFAPP RFPGS
Sbjct: 121 HLATTWLGHDTIALFHSAKVLTFTIVFECLLGIKVEPDVLTTFERVLEGVFAPPIRFPGS 180

Query: 218 KFSRAKKARLEIEKMLREVVREKRRRIEGKVKEAED-GLLLSRMVTAMMRGEISEVEVID 277
           +F RAKKARLEIEKMLR+VVREKRR+IEGK++E E+ G LLSRMV AM+RGEISEVEVID
Sbjct: 181 RFLRAKKARLEIEKMLRKVVREKRRKIEGKLEEEEEGGSLLSRMVMAMIRGEISEVEVID 240

Query: 278 NVVLLVFAAHDTTSFAIAITFKMLAHHSDCYSLLLKEEKCLDSTEHADIARSKEPGQNLT 337
           NVVLLVFAAHDTTSFAIA+ FKMLAHH+DCY+LLL+        EH DI RSK   QNLT
Sbjct: 241 NVVLLVFAAHDTTSFAIAMAFKMLAHHTDCYTLLLR--------EHDDILRSKGEDQNLT 300

Query: 338 MEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIDYEGFFIPKGWKVLWTTYGTHYEEE 397
           MEDIKKMKYTWQVARE++RLFPPIFGSFRKAISDI+YEGFFIPKGWKVLWTTYGTHYEEE
Sbjct: 301 MEDIKKMKYTWQVARETMRLFPPIFGSFRKAISDIEYEGFFIPKGWKVLWTTYGTHYEEE 360

Query: 398 YFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYDWSLVHP 457
           YFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRY+WSL+HP
Sbjct: 361 YFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYNWSLIHP 420

Query: 458 DEPIIMDPLPFPSHGMPIKISPKSEVCHNVQQM 490
           DEPIIMDPLPFPSHGMPIKISPKSEVC N+QQM
Sbjct: 421 DEPIIMDPLPFPSHGMPIKISPKSEVCTNIQQM 445

[Close]

BLAST of Spg007794 vs. ExPASy TrEMBL
Match: A0A6J1HZM9 (taxadiene 5-alpha hydroxylase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468378 PE=3 SV=1)

HSP 1 Score: 787.7 bits (2033), Expect = 2.7e-224
Identity = 400/479 (83.51%), Postives = 429/479 (89.56%), Query Frame = 0


Query: 16  FLAAILIMS-IFLFR----SKNLPPGQMGFPWIGETMDFYRAQRRNRLFEDFVGPRVAKF 75
           FL+AILI+S IF+FR    SK +PPGQMG PWIG+TM FYRAQR+NRLFEDFVGPRVAK+
Sbjct: 4   FLSAILIISIIFVFRSIKGSKKVPPGQMGLPWIGQTMAFYRAQRKNRLFEDFVGPRVAKY 63

Query: 76  GKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHRC 135
           GKIFKTSLMGSPTVV+NGAEANRFFLSNEFKLVVSSWP+ASVQLMGEECIMQKQGESHRC
Sbjct: 64  GKIFKTSLMGSPTVVINGAEANRFFLSNEFKLVVSSWPAASVQLMGEECIMQKQGESHRC 123

Query: 136 VRGLIGASLAGSSLEAMMPKLSSTIRSHLDNNWLGRDIIGLFHSAKVLTFTIVFECLMGI 195
           VRGLI ASLA SSLEAMMPKL +TI+ HLD  W+G DII L+HSAKVLTF+IVFECLMGI
Sbjct: 124 VRGLIAASLASSSLEAMMPKLCTTIQLHLDTKWVGHDIISLYHSAKVLTFSIVFECLMGI 183

Query: 196 GVEPDVLRTFERVLEGVFAPPFRFPGSKFSRAKKARLEIEKMLREVVREKRRRIEGKVKE 255
            VEP +L TFERVLEGVFA PF FPG++FSRAKKAR+EI KMLREVVREKRRRIE +++E
Sbjct: 184 KVEPRMLTTFERVLEGVFALPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEELEE 243

Query: 256 AEDGLLLSRMVTAMMRGEISEVEVIDNVVLLVFAAHDTTSFAIAITFKMLAHHSDCYSLL 315
            E  LLLSRMV AM+RGEISEVEV+DNVVLLVFAAHDTTSFAIA+TFKMLA H  CYSLL
Sbjct: 244 EECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQHPSCYSLL 303

Query: 316 LKEEKCLDSTEHADIARSKEPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISD 375
           L+        EHADI RSK  GQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAI+D
Sbjct: 304 LQ--------EHADIVRSKGAGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITD 363

Query: 376 IDYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAG 435
           I+YEGFFIPKGWKVLWT YGTH E+EYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAG
Sbjct: 364 IEYEGFFIPKGWKVLWTMYGTHLEDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAG 423

Query: 436 YQLAKLNILIFIHFVVTRYDWSLVHPDEPIIMDPLPFPSHGMPIKISPKSEVCHNVQQM 490
           YQLAKLNILIFIH VVTRYDWSL+HPDE IIMDPLP PSHGM  KISPKSEV  NVQQM
Sbjct: 424 YQLAKLNILIFIHLVVTRYDWSLIHPDETIIMDPLPIPSHGMQTKISPKSEVRPNVQQM 474

[Close]

BLAST of Spg007794 vs. ExPASy TrEMBL
Match: A0A6J1GK43 (taxadiene 5-alpha hydroxylase isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111455006 PE=3 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 5.2e-223
Identity = 393/460 (85.43%), Postives = 416/460 (90.43%), Query Frame = 0


Query: 30  SKNLPPGQMGFPWIGETMDFYRAQRRNRLFEDFVGPRVAKFGKIFKTSLMGSPTVVVNGA 89
           SK +PPGQMG PWIGETM FYRAQR+NRLFE+FVGPRVAK+GKIFKTSLMGSPTVVVNGA
Sbjct: 23  SKKVPPGQMGLPWIGETMVFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGA 82

Query: 90  EANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIGASLAGSSLEAMMP 149
           EANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLI ASLA SSLEAMMP
Sbjct: 83  EANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSLEAMMP 142

Query: 150 KLSSTIRSHLDNNWLGRDIIGLFHSAKVLTFTIVFECLMGIGVEPDVLRTFERVLEGVFA 209
           KL +TI+ +LD  WLG DII L+HSAKVLTFTIVFECLMGI VEP +L TFERVLEGVFA
Sbjct: 143 KLCTTIQLYLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFA 202

Query: 210 PPFRFPGSKFSRAKKARLEIEKMLREVVREKRRRIEGKVKEAEDGLLLSRMVTAMMRGEI 269
           PPF FPG++FSRAKKAR+EI KMLREVVREKRRRIE +  E E  LLLSRMV AM+RGEI
Sbjct: 203 PPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEEPVEEECELLLSRMVRAMIRGEI 262

Query: 270 SEVEVIDNVVLLVFAAHDTTSFAIAITFKMLAHHSDCYSLLLKEEKCLDSTEHADIARSK 329
           SEVEV+DNVVLLVFAAHDTTSFAIA+TFKMLA   +CYSLLL+        EHADI RSK
Sbjct: 263 SEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQ--------EHADIVRSK 322

Query: 330 EPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIDYEGFFIPKGWKVLWTTY 389
             GQN+TMEDIKKMKYTWQVARESIRLFPPIFGSFRKAI+DI+YEGFFIPKGWKVLWTTY
Sbjct: 323 GAGQNMTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTY 382

Query: 390 GTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRY 449
           GTH E++YFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIH VVTRY
Sbjct: 383 GTHLEDKYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRY 442

Query: 450 DWSLVHPDEPIIMDPLPFPSHGMPIKISPKSEVCHNVQQM 490
           DWSLVHPDE IIMDPLP PSHGMPIKISPKSE   NVQQM
Sbjct: 443 DWSLVHPDETIIMDPLPIPSHGMPIKISPKSEARPNVQQM 474

[Close]

BLAST of Spg007794 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 596.7 bits (1537), Expect = 1.7e-170
Identity = 297/485 (61.24%), Postives = 367/485 (75.67%), Query Frame = 0


Query: 8   FLLSLSLSFLAAILIMSIFLFRSKN-------------LPPGQMGFPWIGETMDFYRAQR 67
           F+LS     +AA +  ++F FR K+             L PG+MG PWIGETMDFY+AQ+
Sbjct: 7   FVLSWVFLCIAATISSTLFFFRKKHHRFITKKIQKKKKLLPGEMGLPWIGETMDFYKAQK 66

Query: 68  RNRLFEDFVGPRVAKFGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQL 127
            NR+FEDFV PR+ K G IFKT +MGSPT+VVNGAEANR  LSNEF LVVSSWPS+SVQL
Sbjct: 67  SNRVFEDFVNPRIIKHGNIFKTRIMGSPTIVVNGAEANRLILSNEFSLVVSSWPSSSVQL 126

Query: 128 MGEECIMQKQGESHRCVRGLIGASLAGSSLEAMMPKLSSTIRSHLDNNWLGRDIIGLFHS 187
           MG  CIM KQGE HR +RG++  SL+   LE+++PKL  T++ H +  W G++ I L+ S
Sbjct: 127 MGMNCIMAKQGEKHRVLRGIVANSLSYIGLESLIPKLCDTVKFHHETEWRGKEEISLYRS 186

Query: 188 AKVLTFTIVFECLMGIGVEPDVLRTFERVLEGVFAPPFRFPGSKFSRAKKARLEIEKMLR 247
           AKVLTFT+VFECL GI VE  +L  FERVLEGVFA P  FP SKF+RAKKARLEIE  L 
Sbjct: 187 AKVLTFTVVFECLYGIKVEIGMLEVFERVLEGVFALPVEFPCSKFARAKKARLEIETFLV 246

Query: 248 EVVREKRRRIEGKVKEAEDGLLLSRMVTAMMRGEISEVEVIDNVVLLVFAAHDTTSFAIA 307
             VREKRR +E +  E  +  L SR+V  +++G I+E EV+DN+VLLVFAAHDTTS+A++
Sbjct: 247 GKVREKRREMEKEGAEKPNTTLFSRLVEELIKGVITEEEVVDNMVLLVFAAHDTTSYAMS 306

Query: 308 ITFKMLAHHSDCYSLLLKEEKCLDSTEHADIARSKEPGQNLTMEDIKKMKYTWQVARESI 367
           +TFKMLA H  C   LL+        EHA I  +K  G+ LT+ED+KKMKY+WQV RE++
Sbjct: 307 MTFKMLAQHPTCRDTLLQ--------EHAQIKANKGEGEYLTVEDVKKMKYSWQVVRETM 366

Query: 368 RLFPPIFGSFRKAISDIDYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVS 427
           RL PPIFGSFRKA++DIDY G+ IPKGWK+LWTTYGTHY  E F+DP+SF P+RF++P+ 
Sbjct: 367 RLSPPIFGSFRKAVADIDYGGYTIPKGWKILWTTYGTHYNPEIFQDPMSFDPTRFDKPIQ 426

Query: 428 QYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYDWSLVHPDEPIIMDPLPFPSHGMPI 480
            Y Y+PFGGGPR CAG+QLAK++IL+F+HFVVT +DWSLV+PDE I MDPLPFPS GMPI
Sbjct: 427 AYTYLPFGGGPRLCAGHQLAKISILVFMHFVVTGFDWSLVYPDETISMDPLPFPSLGMPI 483

[Close]

BLAST of Spg007794 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 298.1 bits (762), Expect = 1.3e-80
Identity = 178/491 (36.25%), Postives = 267/491 (54.38%), Query Frame = 0


Query: 8   FLLSLSLSFLAAILIMSIFLFRS-------KNLPPGQMGFPWIGETMDFYRAQRRNRLFE 67
           ++  + + FL++IL+  + L R         NLPPG  G P IGE+  F  A R+    E
Sbjct: 2   YMAIMIILFLSSILLSLLLLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHP-E 61

Query: 68  DFVGPRVAKFGK----IFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMG 127
            F+  RV +F      +FKT L GSPT VV GA  N+F  +NE KLVVS WP  SV  + 
Sbjct: 62  KFITDRVRRFSSSSSCVFKTHLFGSPTAVVTGASGNKFLFTNENKLVVSWWPD-SVNKIF 121

Query: 128 EECIMQKQGESHRCVRGLIGASLAGSSLEAMMPKLSSTIRSHLDNNWLGRDIIGLFHSAK 187
              +     E  R +R L+   +   +L   +  +    + H +  W  +D + +F   K
Sbjct: 122 PSSMQTSSKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTK 181

Query: 188 VLTFTIVFECLMGIGVEPDVLR----TFERVLEGVFAPPFRFPGSKFSRAKKARLEIEKM 247
             TF+I     + +  +P  +R     F  V  G+F+ P   PG++F+RA KA   + K 
Sbjct: 182 KFTFSIACRSFLSM-EDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKE 241

Query: 248 LREVVREKRRRIE-GKVKEAEDGLLLSRMVTAMMRGEISEVEVIDNVVLLVFAAHDTTSF 307
           +  +VR+++  ++ GK  E  D  +LS M+  M  GE  + ++ D ++ L+   HDT S 
Sbjct: 242 VSAIVRQRKEELKAGKALEEHD--ILSHML--MNIGETKDEDLADKIIGLLIGGHDTASI 301

Query: 308 AIAITFKMLAHHSDCYSLLLKEEKCLDSTEHADIARSKEPGQNLTMEDIKKMKYTWQVAR 367
                   LA     Y  +L+E+K        +I + K+  + L  EDI+KM+Y+W VA 
Sbjct: 302 VCTFVVNYLAEFPHVYQRVLQEQK--------EILKEKKEKEGLRWEDIEKMRYSWNVAC 361

Query: 368 ESIRLFPPIFGSFRKAISDIDYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFE- 427
           E +R+ PP+ G+FR+AI    ++GF+IPKGWK+ W+   TH   +YF +P  F+P+RFE 
Sbjct: 362 EVMRIVPPLSGTFREAIDHFSFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEG 421

Query: 428 EPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYDWSLVHPDE-PIIMDPLPFPS 481
                Y YVPFGGGPR C G + A+L ILIF+H +V R+ W  V P+E  I++DPLP P 
Sbjct: 422 SGPKPYTYVPFGGGPRMCPGKEYARLEILIFMHNLVNRFKWEKVFPNENKIVVDPLPIPD 477

[Close]

BLAST of Spg007794 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 244.6 bits (623), Expect = 1.7e-64
Identity = 147/479 (30.69%), Postives = 255/479 (53.24%), Query Frame = 0


Query: 9   LLSLSLSFLAAILIMSIFLF---RSKNLPPGQMGFPWIGETMDFYRAQRRNRLFEDFVGP 68
           L++L++  +  +L+   +L    +   LPPG MG P+IGET+  Y     +     F   
Sbjct: 22  LITLTIVVVVVVLLFKWWLHWKEQRLRLPPGSMGLPYIGETLRLYTENPNS-----FFAT 81

Query: 69  RVAKFGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQG 128
           R  K+G IFKT ++G P V+++  EA R  L ++  L   ++P +  +++G E +   QG
Sbjct: 82  RQNKYGDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQG 141

Query: 129 ESHRCVRGLIGASLAGSSLEAMMPKLSSTIRSHLDNNWLGRDIIGLFHSAKVLTFTIVFE 188
             H  ++ L+ +S   S+L   +  +   +   L ++W  +  I      K   F +   
Sbjct: 142 PYHSTLKRLVQSSFMPSALRPTVSHIELLVLQTL-SSWTSQKSINTLEYMKRYAFDVAIM 201

Query: 189 CLMGIGVEP---DVLR-TFERVLEGVFAPPFRFPGSKFSRAKKARLEIEKMLREVVREKR 248
              G   EP   DV++  ++R+  G  + P   PG+ F ++ KAR+E+ + LR+V+  ++
Sbjct: 202 SAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVI--EK 261

Query: 249 RRIEGKVKEAEDGLLLSRMVTA--MMRGEISEVEVIDNVVLLVFAAHDTTSFAIAITFKM 308
           RR  G+    E+G LL  ++ A    R  +S+ ++ DN++ ++FAA DTT+  +    K 
Sbjct: 262 RRENGR----EEGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKY 321

Query: 309 LAHHSDCYSLLLKEEKCLDSTEHADIARSKEPGQNLTMEDIKKMKYTWQVARESIRLFPP 368
           L  H +    + +E+  +         + K+  + ++ ED +KM  T +V +E++R    
Sbjct: 322 LHDHPNLLQEVSREQFSIRQ-------KIKKENRRISWEDTRKMPLTTRVIQETLRAASV 381

Query: 369 IFGSFRKAISDIDYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYV 428
           +  +FR+A+ D++Y+G+ IPKGWKVL      H+  E+F DP  F PSRFE     Y Y+
Sbjct: 382 LSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEVAPKPYTYM 441

Query: 429 PFGGGPRACAGYQLAKLNILIFIHFVVTRYDWSLVHPDEPIIMDPLPFPSHGMPIKISP 479
           PFG G  +C G +LAKL +LI +H + T + W ++  +E I   P P P  G+PI+++P
Sbjct: 442 PFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481

[Close]

BLAST of Spg007794 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 244.6 bits (623), Expect = 1.7e-64
Identity = 147/479 (30.69%), Postives = 255/479 (53.24%), Query Frame = 0


Query: 9   LLSLSLSFLAAILIMSIFLF---RSKNLPPGQMGFPWIGETMDFYRAQRRNRLFEDFVGP 68
           L++L++  +  +L+   +L    +   LPPG MG P+IGET+  Y     +     F   
Sbjct: 22  LITLTIVVVVVVLLFKWWLHWKEQRLRLPPGSMGLPYIGETLRLYTENPNS-----FFAT 81

Query: 69  RVAKFGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQG 128
           R  K+G IFKT ++G P V+++  EA R  L ++  L   ++P +  +++G E +   QG
Sbjct: 82  RQNKYGDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQG 141

Query: 129 ESHRCVRGLIGASLAGSSLEAMMPKLSSTIRSHLDNNWLGRDIIGLFHSAKVLTFTIVFE 188
             H  ++ L+ +S   S+L   +  +   +   L ++W  +  I      K   F +   
Sbjct: 142 PYHSTLKRLVQSSFMPSALRPTVSHIELLVLQTL-SSWTSQKSINTLEYMKRYAFDVAIM 201

Query: 189 CLMGIGVEP---DVLR-TFERVLEGVFAPPFRFPGSKFSRAKKARLEIEKMLREVVREKR 248
              G   EP   DV++  ++R+  G  + P   PG+ F ++ KAR+E+ + LR+V+  ++
Sbjct: 202 SAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVI--EK 261

Query: 249 RRIEGKVKEAEDGLLLSRMVTA--MMRGEISEVEVIDNVVLLVFAAHDTTSFAIAITFKM 308
           RR  G+    E+G LL  ++ A    R  +S+ ++ DN++ ++FAA DTT+  +    K 
Sbjct: 262 RRENGR----EEGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKY 321

Query: 309 LAHHSDCYSLLLKEEKCLDSTEHADIARSKEPGQNLTMEDIKKMKYTWQVARESIRLFPP 368
           L  H +    + +E+  +         + K+  + ++ ED +KM  T +V +E++R    
Sbjct: 322 LHDHPNLLQEVSREQFSIRQ-------KIKKENRRISWEDTRKMPLTTRVIQETLRAASV 381

Query: 369 IFGSFRKAISDIDYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYV 428
           +  +FR+A+ D++Y+G+ IPKGWKVL      H+  E+F DP  F PSRFE     Y Y+
Sbjct: 382 LSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEVAPKPYTYM 441

Query: 429 PFGGGPRACAGYQLAKLNILIFIHFVVTRYDWSLVHPDEPIIMDPLPFPSHGMPIKISP 479
           PFG G  +C G +LAKL +LI +H + T + W ++  +E I   P P P  G+PI+++P
Sbjct: 442 PFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481

[Close]

BLAST of Spg007794 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 244.2 bits (622), Expect = 2.2e-64
Identity = 148/478 (30.96%), Postives = 256/478 (53.56%), Query Frame = 0


Query: 8   FLLSLSLSFLAAILIMSIFLFRSKNLPPGQMGFPWIGETMDFYRAQRRNRLFEDFVGPRV 67
           F  SL L FL  ++    F      LPPG MG+P++GET   Y +Q  N  F+     + 
Sbjct: 11  FAGSLFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLY-SQDPNVFFQS----KQ 70

Query: 68  AKFGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGES 127
            ++G +FKT ++G P V+++  EA +F L  +  L   ++P++  +++G++ I   QG+ 
Sbjct: 71  KRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDY 130

Query: 128 HRCVRGLIGASLAGSSLEAMMPKLSSTIRSHLDNNWLGRDIIGLFHSAKVLTFTIVFECL 187
           H  +R L+  +    S+  M+P + S  +  L  +W G  +I  +   K  TF +    +
Sbjct: 131 HAKLRKLVLRAFMPESIRNMVPDIESIAQDSL-RSWEG-TMINTYQEMKTYTFNVALLSI 190

Query: 188 MG---IGVEPDVLRTFERVLEGVFAPPFRFPGSKFSRAKKARLEIEKMLREVVREKRRRI 247
            G   +    D+ R +  + +G  + P   PG+ F ++ KAR E+ ++L  ++ E+R+  
Sbjct: 191 FGKDEVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQN- 250

Query: 248 EGKVKEAEDGLLLSRMVTAMMRGEISEVEVIDNVVLLVFAAHDTTSFAIAITFKMLAHHS 307
                 + + LL S M     + E+++ ++ DN++ ++FAA DTT+  ++   K LA + 
Sbjct: 251 ----GSSHNDLLGSFMGD---KEELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENP 310

Query: 308 DCYSLLLKEEKCLDSTEHADIARSKEPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSF 367
           +    + +E+          I + KE G++LT  D KKM  T +V +E++R+   +  +F
Sbjct: 311 NVLEAVTEEQMA--------IRKDKEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTF 370

Query: 368 RKAISDIDYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGG 427
           R+A+ D++YEG+ IPKGWKVL      H+  + F +P  F PSRFE       ++PFG G
Sbjct: 371 REAVEDVEYEGYLIPKGWKVLPLFRNIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNG 430

Query: 428 PRACAGYQLAKLNILIFIHFVVTRYDWSLVHPDEPIIMDPLPFPSHGMPIKISPKSEV 483
             +C G +LAKL + I IH + T+Y WS+V   + I   P   P +G+PI ++ K E+
Sbjct: 431 THSCPGNELAKLEMSIMIHHLTTKYSWSIVGASDGIQYGPFALPQNGLPIVLARKPEI 465

The following BLAST results are available for this feature:

BLAST of Spg007794 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147913.1	2.9e-236	84.54	taxadiene 5-alpha hydroxylase isoform X1 [Momordica charantia]	[more]
XP_023554746.1	2.4e-230	83.81	taxadiene 5-alpha hydroxylase isoform X2 [Cucurbita pepo subsp. pepo]	[more]
KAG7012036.1	3.1e-230	83.40	hypothetical protein SDJN02_26944, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022952282.1	2.0e-229	83.60	taxadiene 5-alpha hydroxylase isoform X2 [Cucurbita moschata]	[more]
XP_022147914.1	3.9e-225	86.53	taxadiene 5-alpha hydroxylase isoform X2 [Momordica charantia] >XP_022147915.1 t...	[more]

BLAST of Spg007794 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6WG30	3.2e-97	40.58	Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2	[more]
Q50EK0	1.2e-91	38.75	Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1	[more]
Q50EK1	4.5e-91	38.45	Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1	[more]
Q8W4T9	1.7e-90	37.88	Taxane 13-alpha-hydroxylase OS=Taxus cuspidata OX=99806 GN=CYP725A2 PE=1 SV=1	[more]
Q84KI1	3.3e-89	38.08	Taxoid 14-beta-hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=1	[more]

BLAST of Spg007794 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D2L6	1.4e-236	84.54	taxadiene 5-alpha hydroxylase isoform X1 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1GJY9	9.7e-230	83.60	taxadiene 5-alpha hydroxylase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1D1G7	1.9e-225	86.53	taxadiene 5-alpha hydroxylase isoform X2 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1HZM9	2.7e-224	83.51	taxadiene 5-alpha hydroxylase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11146...	[more]
A0A6J1GK43	5.2e-223	85.43	taxadiene 5-alpha hydroxylase isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]

BLAST of Spg007794 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G42850.1	1.7e-170	61.24	cytochrome P450, family 718	[more]
AT5G36110.1	1.3e-80	36.25	cytochrome P450, family 716, subfamily A, polypeptide 1	[more]
AT2G29090.1	1.7e-64	30.69	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]
AT2G29090.2	1.7e-64	30.69	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]
AT4G19230.1	2.2e-64	30.96	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 222..242
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 12..460 e-value: 2.1E-13 score: 47.5
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 21..478
None	No IPR available	PANTHER	PTHR24286:SF221	TAXADIENE 5-ALPHA HYDROXYLASE	coord: 21..478
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 428..451 score: 22.5 coord: 273..290 score: 28.88 coord: 67..86 score: 27.83 coord: 387..411 score: 26.98 coord: 418..428 score: 50.41
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 428..439 score: 33.52 coord: 284..301 score: 29.68 coord: 348..359 score: 32.64 coord: 419..428 score: 58.89
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 34..464 e-value: 2.5E-52 score: 178.2
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 26..479 e-value: 1.9E-107 score: 361.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 26..478
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 421..430

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg007794.1	Spg007794.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen