HG10014122 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10014122
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Alpha-amylase
Location	Chr02: 7766349 .. 7767911 (-)
RNA-Seq Expression	HG10014122
Synteny	HG10014122

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGCTTATAAAACTGAAATTCCCACTGCCCTTTTAACAAACAATGGAGTTTCTAGCTTCTCTGTGTTTCCTCTACCTCTCTGTTTCCCTCTTTCCATCCCCTTCTTCTTCTACCTTACTGTTTCAGGTAACCAAATTCACTTTACTACTTTACTTCTTCATGGAAACTCACTTTTTAAAAAGATTGAAGTTGATTGTAATGAAACAGGGCTTCAACTGGGAATCAAGCAGCAAAGGAGGATGGTACAACTCACTACACAACATAATTCCTGACCTTGTAAGTGCTGAAGTTACACATGTCTGGCTTCCTCCACCCTCCCAATCAGTTTCTTCACAAGGCAAGCCTTCTTTCTCTCTGTTCCTTCAGCTCATTATCATCAATATCTGTTTAAATTTGTATAAACCCTTTTACAGGATACTTACCTGGAAGGCTTTATGATCTTAATGCATCCAAATACGGAACCCAGGATGAACTCAAGTCCCTAATTGCCGCTCTTCATCAGAAGGGAATCAAAGCCATAGCTGACATAGTAATCAACCACAGAACAGCAGAGAAACAAGACGAGAGAGGAATTTGGTGCATTTTTGAAGGGGGAACTCCAGACAGCCGCCTTGATTGGGGCCCTTCCTTCATTTGCAGTGATGACACTGAATATTCCGATGGGAAGGGCAATCCAGACAGTGGGGAACCCTATCCACCAGCACCTGATATCGATCATCTCAATCCACAAGTTCAGAAAGAAATATCAGAATGGATGAGATGGTTGAAAACAGAAATAGGGTTTGATGGATGGAGATTCGATTTCGTAAAGGGCTATGCACCAAGTATTACAAAACTCTACATGGATCAAACTTTACCAGATTTTGCAGTTGGTGAGAAATGGGATTCACTTTCAGAAGGACAGGACAGGAAACCCAATCAAAATCAAGACGCACATCGCGACGCTCTTGCAAATTGGGTTAGGGCTTCGGGCGATACGGTCACAGCATTTGATTTCACAACTAAAGGGATTCTTCAAGCTGCCGTTCAAGGAGAGTTATGGAGGTTGAAGGATTCTAACGGGAAGCCGCCGGGATTCATCGGAATCAAGCCGGAAAGTGCCGTAACTTTCATCGATAACCACGACACTGGTTCGACGCAAAGAATGTGGCCGTTCCCTTCCGATAAAGTGATTCAAGGATACGCATACATTCTCACTCATCCTGGAACGCCTTCCATAGTAATTCATTCATTCAACAAAAACAGTTTAGATATTTGAGTTTCGAAACTTAATTACCTGTAATTCACATACTGTTGGTTTGTTGCAGTTTTACGACCACTTCGTTGATTGGGGATTGAAGGAGGAGATAATGAAATTGAGTGCAATTAGGGCAAGGAATGGGATTGGGAAAACGAGCACGGTGAATATTATGGCTTCTGAGGGGGATTTGTACGTTGCTGGTGTTGATGGGAAAATTATAATGAAGATTGGAGCGAACACCAATCTTGGAAATCTGATTCCGTCGGATTATCAGCTTGCCACTTCTGGAATGGAGTACGCTGTTTGGGAAAGAAACTGA

mRNA sequence

ATGGAAGCTTATAAAACTGAAATTCCCACTGCCCTTTTAACAAACAATGGAGTTTCTAGCTTCTCTGTGTTTCCTCTACCTCTCTGTTTCCCTCTTTCCATCCCCTTCTTCTTCTACCTTACTGTTTCAGGATACTTACCTGGAAGGCTTTATGATCTTAATGCATCCAAATACGGAACCCAGGATGAACTCAAGTCCCTAATTGCCGCTCTTCATCAGAAGGGAATCAAAGCCATAGCTGACATAGTAATCAACCACAGAACAGCAGAGAAACAAGACGAGAGAGGAATTTGGTGCATTTTTGAAGGGGGAACTCCAGACAGCCGCCTTGATTGGGGCCCTTCCTTCATTTGCAGTGATGACACTGAATATTCCGATGGGAAGGGCAATCCAGACAGTGGGGAACCCTATCCACCAGCACCTGATATCGATCATCTCAATCCACAAGTTCAGAAAGAAATATCAGAATGGATGAGATGGTTGAAAACAGAAATAGGGTTTGATGGATGGAGATTCGATTTCGTAAAGGGCTATGCACCAAGTATTACAAAACTCTACATGGATCAAACTTTACCAGATTTTGCAGTTGGTGAGAAATGGGATTCACTTTCAGAAGGACAGGACAGGAAACCCAATCAAAATCAAGACGCACATCGCGACGCTCTTGCAAATTGGGTTAGGGCTTCGGGCGATACGGTCACAGCATTTGATTTCACAACTAAAGGGATTCTTCAAGCTGCCGTTCAAGGAGAGTTATGGAGGTTGAAGGATTCTAACGGGAAGCCGCCGGGATTCATCGGAATCAAGCCGGAAAGTGCCGTAACTTTCATCGATAACCACGACACTGGTTCGACGCAAAGAATGTGGCCGTTCCCTTCCGATAAAGTGATTCAAGGATACGCATACATTCTCACTCATCCTGGAACGCCTTCCATATTTTACGACCACTTCGTTGATTGGGGATTGAAGGAGGAGATAATGAAATTGAGTGCAATTAGGGCAAGGAATGGGATTGGGAAAACGAGCACGGTGAATATTATGGCTTCTGAGGGGGATTTGTACGTTGCTGGTGTTGATGGGAAAATTATAATGAAGATTGGAGCGAACACCAATCTTGGAAATCTGATTCCGTCGGATTATCAGCTTGCCACTTCTGGAATGGAGTACGCTGTTTGGGAAAGAAACTGA

Coding sequence (CDS)

ATGGAAGCTTATAAAACTGAAATTCCCACTGCCCTTTTAACAAACAATGGAGTTTCTAGCTTCTCTGTGTTTCCTCTACCTCTCTGTTTCCCTCTTTCCATCCCCTTCTTCTTCTACCTTACTGTTTCAGGATACTTACCTGGAAGGCTTTATGATCTTAATGCATCCAAATACGGAACCCAGGATGAACTCAAGTCCCTAATTGCCGCTCTTCATCAGAAGGGAATCAAAGCCATAGCTGACATAGTAATCAACCACAGAACAGCAGAGAAACAAGACGAGAGAGGAATTTGGTGCATTTTTGAAGGGGGAACTCCAGACAGCCGCCTTGATTGGGGCCCTTCCTTCATTTGCAGTGATGACACTGAATATTCCGATGGGAAGGGCAATCCAGACAGTGGGGAACCCTATCCACCAGCACCTGATATCGATCATCTCAATCCACAAGTTCAGAAAGAAATATCAGAATGGATGAGATGGTTGAAAACAGAAATAGGGTTTGATGGATGGAGATTCGATTTCGTAAAGGGCTATGCACCAAGTATTACAAAACTCTACATGGATCAAACTTTACCAGATTTTGCAGTTGGTGAGAAATGGGATTCACTTTCAGAAGGACAGGACAGGAAACCCAATCAAAATCAAGACGCACATCGCGACGCTCTTGCAAATTGGGTTAGGGCTTCGGGCGATACGGTCACAGCATTTGATTTCACAACTAAAGGGATTCTTCAAGCTGCCGTTCAAGGAGAGTTATGGAGGTTGAAGGATTCTAACGGGAAGCCGCCGGGATTCATCGGAATCAAGCCGGAAAGTGCCGTAACTTTCATCGATAACCACGACACTGGTTCGACGCAAAGAATGTGGCCGTTCCCTTCCGATAAAGTGATTCAAGGATACGCATACATTCTCACTCATCCTGGAACGCCTTCCATATTTTACGACCACTTCGTTGATTGGGGATTGAAGGAGGAGATAATGAAATTGAGTGCAATTAGGGCAAGGAATGGGATTGGGAAAACGAGCACGGTGAATATTATGGCTTCTGAGGGGGATTTGTACGTTGCTGGTGTTGATGGGAAAATTATAATGAAGATTGGAGCGAACACCAATCTTGGAAATCTGATTCCGTCGGATTATCAGCTTGCCACTTCTGGAATGGAGTACGCTGTTTGGGAAAGAAACTGA

Protein sequence

MEAYKTEIPTALLTNNGVSSFSVFPLPLCFPLSIPFFFYLTVSGYLPGRLYDLNASKYGTQDELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALANWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTSTVNIMASEGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAVWERN

Homology

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BLAST of HG10014122 vs. NCBI nr
Match: XP_038896126.1 (alpha-amylase-like [Benincasa hispida])

HSP 1 Score: 709.1 bits (1829), Expect = 2.1e-200
Identity = 331/351 (94.30%), Postives = 342/351 (97.44%), Query Frame = 0


Query: 44  GYLPGRLYDLNASKYGTQDELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEG 103
           GYLPGRLYDLNASKYGTQ+ELKSLIA LH+KGIKAIADIVINHRTAEKQD+RGIWCIFEG
Sbjct: 73  GYLPGRLYDLNASKYGTQNELKSLIATLHEKGIKAIADIVINHRTAEKQDQRGIWCIFEG 132

Query: 104 GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKT 163
           GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKE+SEWMRWLKT
Sbjct: 133 GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKELSEWMRWLKT 192

Query: 164 EIGFDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALA 223
           EIGFDGWRFDFVKGYAPSITKLYMDQT P+FAVGEKWD LS GQD KPNQNQD+HRDALA
Sbjct: 193 EIGFDGWRFDFVKGYAPSITKLYMDQTSPEFAVGEKWDGLSYGQDGKPNQNQDSHRDALA 252

Query: 224 NWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTG 283
           NWVRASGD VTAFDFTTKGILQAAV+GELWRLKDSNG PPGFIGIKPESAVTFI+NHDTG
Sbjct: 253 NWVRASGDIVTAFDFTTKGILQAAVEGELWRLKDSNGNPPGFIGIKPESAVTFIENHDTG 312

Query: 284 STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTST 343
           STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLS IRARNGIGKTST
Sbjct: 313 STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSGIRARNGIGKTST 372

Query: 344 VNIMASEGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 395
           VNIMASEGDLYVAGVDGKIIMKIGANT+LGNLIPSDYQLAT GM+YAVWE+
Sbjct: 373 VNIMASEGDLYVAGVDGKIIMKIGANTSLGNLIPSDYQLATYGMDYAVWEK 423

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BLAST of HG10014122 vs. NCBI nr
Match: XP_022142476.1 (alpha-amylase [Momordica charantia])

HSP 1 Score: 691.0 bits (1782), Expect = 5.8e-195
Identity = 319/351 (90.88%), Postives = 341/351 (97.15%), Query Frame = 0


Query: 44  GYLPGRLYDLNASKYGTQDELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEG 103
           GYLPGRLYDL+ASKYGTQ+ELKSLIAALH+KGIKAIADIVINHRTAEKQD+RGIWCIFEG
Sbjct: 87  GYLPGRLYDLDASKYGTQNELKSLIAALHEKGIKAIADIVINHRTAEKQDQRGIWCIFEG 146

Query: 104 GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKT 163
           GTPD+RLDWGPSFICSDDT+YSDGKGNPDSGEPYPPAPDIDHL PQVQKEISEWMRWLKT
Sbjct: 147 GTPDNRLDWGPSFICSDDTDYSDGKGNPDSGEPYPPAPDIDHLKPQVQKEISEWMRWLKT 206

Query: 164 EIGFDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALA 223
           EIGFDGWRFDFVKGYAPS+TKLYMDQT PDFAVGEKWDSLS+G D K NQNQDAHRD+LA
Sbjct: 207 EIGFDGWRFDFVKGYAPSVTKLYMDQTSPDFAVGEKWDSLSDGPDGKLNQNQDAHRDSLA 266

Query: 224 NWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTG 283
           NWV+ASGD VTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTF+DNHDTG
Sbjct: 267 NWVKASGDAVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFVDNHDTG 326

Query: 284 STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTST 343
           STQ++WPFPSDKVIQGYAYILTHPGTP+IFYDHFV+WGLKEEI++LS IRARN IGKTS 
Sbjct: 327 STQKLWPFPSDKVIQGYAYILTHPGTPAIFYDHFVEWGLKEEIIRLSEIRARNRIGKTSA 386

Query: 344 VNIMASEGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 395
           VNI+AS+ DLYVAGVDGKIIMKIGANTNLGNLIPS++QLATSGMEYAVWE+
Sbjct: 387 VNILASDADLYVAGVDGKIIMKIGANTNLGNLIPSEFQLATSGMEYAVWEK 437

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BLAST of HG10014122 vs. NCBI nr
Match: XP_023536126.1 (alpha-amylase, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 688.0 bits (1774), Expect = 4.9e-194
Identity = 319/351 (90.88%), Postives = 334/351 (95.16%), Query Frame = 0


Query: 44  GYLPGRLYDLNASKYGTQDELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEG 103
           GYLPGRLYDLNASKYGTQDELKSLIAALH  GIKA+ADIVINHRTA+KQDERGIWCIFEG
Sbjct: 116 GYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTADKQDERGIWCIFEG 175

Query: 104 GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKT 163
           GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPY  APDIDHLNPQVQ E+SEWMRWLKT
Sbjct: 176 GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYSAAPDIDHLNPQVQNELSEWMRWLKT 235

Query: 164 EIGFDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALA 223
           EIGFDGWRFDFVKGYAP ITKLYMDQT PDFAVGEKWDSLS G D KP+ NQD HR+ALA
Sbjct: 236 EIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGKPDPNQDGHREALA 295

Query: 224 NWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTG 283
           NWVR SGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTG
Sbjct: 296 NWVRDSGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTG 355

Query: 284 STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTST 343
           STQ++WPFPSDKVIQGYAYILTHPGTPSIFYDHFV+WGLKEE++KLSAIR RNGIG+ ST
Sbjct: 356 STQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLSAIRTRNGIGERST 415

Query: 344 VNIMASEGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 395
           VNIMAS+GDLYVAG+DGKIIMKIG NTNLGNLIPSDY LATSGM+YAVWE+
Sbjct: 416 VNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLIPSDYVLATSGMQYAVWEK 466

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BLAST of HG10014122 vs. NCBI nr
Match: XP_022976296.1 (alpha-amylase [Cucurbita maxima])

HSP 1 Score: 687.2 bits (1772), Expect = 8.4e-194
Identity = 318/351 (90.60%), Postives = 334/351 (95.16%), Query Frame = 0


Query: 44  GYLPGRLYDLNASKYGTQDELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEG 103
           GYLPGRLYDLNASKYGTQDELKSLIAALH  GIKA+ADIVINHRTA+KQDERGIWCIFEG
Sbjct: 74  GYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTADKQDERGIWCIFEG 133

Query: 104 GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKT 163
           GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPY  APDIDHLNPQVQ E+SEWMRWLKT
Sbjct: 134 GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYSAAPDIDHLNPQVQNELSEWMRWLKT 193

Query: 164 EIGFDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALA 223
           EIGFDGWRFDFVKGYAP ITKLYMDQT PDFAVGEKWDSLS G D KP+ NQD HR+ALA
Sbjct: 194 EIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSPGPDGKPDPNQDGHREALA 253

Query: 224 NWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTG 283
           NWVR SGDTVTAFDFTTKGILQAAVQGELWRLKD NGKPPGFIGIKPESAVTFIDNHDTG
Sbjct: 254 NWVRDSGDTVTAFDFTTKGILQAAVQGELWRLKDPNGKPPGFIGIKPESAVTFIDNHDTG 313

Query: 284 STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTST 343
           STQ++WPFPSDKVIQGYAYILTHPGTPSIFYDHFV+WGLKEE++KLSAIR RNGIG+ ST
Sbjct: 314 STQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLSAIRTRNGIGERST 373

Query: 344 VNIMASEGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 395
           VNIMAS+GDLYVAG+DGKIIMKIG NTNLGNLIPSDY+LATSGM+YAVWE+
Sbjct: 374 VNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLIPSDYELATSGMQYAVWEK 424

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BLAST of HG10014122 vs. NCBI nr
Match: XP_022936258.1 (alpha-amylase, partial [Cucurbita moschata])

HSP 1 Score: 686.8 bits (1771), Expect = 1.1e-193
Identity = 317/351 (90.31%), Postives = 335/351 (95.44%), Query Frame = 0


Query: 44  GYLPGRLYDLNASKYGTQDELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEG 103
           GYLPGRLYDLNASKYGTQ+ELKSLIAALH  GIKA+ADIVINHRTA+KQDERGIWCIFEG
Sbjct: 98  GYLPGRLYDLNASKYGTQNELKSLIAALHNNGIKAVADIVINHRTADKQDERGIWCIFEG 157

Query: 104 GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKT 163
           GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPY  APDIDHLNPQVQ E+SEWMRWLKT
Sbjct: 158 GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYSAAPDIDHLNPQVQNELSEWMRWLKT 217

Query: 164 EIGFDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALA 223
           EIGFDGWRFDFVKGYAP ITKLYMDQT PDFAVGEKWDSLS G D KP+ NQD HR+ALA
Sbjct: 218 EIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGKPDPNQDGHREALA 277

Query: 224 NWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTG 283
           NWVR SGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTG
Sbjct: 278 NWVRDSGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTG 337

Query: 284 STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTST 343
           STQ++WPFPSDKVIQGYAYILTHPGTPSIFYDHFV+WGLKEE++KLSAIRARNGIG+ ST
Sbjct: 338 STQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLSAIRARNGIGERST 397

Query: 344 VNIMASEGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 395
           VNIMAS+GDLYVAG+DGKIIMKIG NTNLGNL+PSDY+LA SGM+YAVWE+
Sbjct: 398 VNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYAVWEK 448

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BLAST of HG10014122 vs. ExPASy Swiss-Prot
Match: P17859 (Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 5.0e-173
Identity = 279/350 (79.71%), Postives = 312/350 (89.14%), Query Frame = 0


Query: 44  GYLPGRLYDLNASKYGTQDELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEG 103
           GYLPGRLYDL+ASKYG+++ELKSLIAA H+KGIK +ADIVINHRTAE++D RGI+CIFEG
Sbjct: 72  GYLPGRLYDLDASKYGSKNELKSLIAAFHEKGIKCLADIVINHRTAERKDGRGIYCIFEG 131

Query: 104 GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKT 163
           GTPDSR DWGPSFIC DDT YSDG GN DSGE Y  APDIDHLNPQVQ+E+SEWM WLKT
Sbjct: 132 GTPDSRQDWGPSFICRDDTAYSDGTGNNDSGEGYDAAPDIDHLNPQVQRELSEWMNWLKT 191

Query: 164 EIGFDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALA 223
           EIGFDGWRFDFVKGYAPSI+K+YM+QT PDFAVGEKWDS+S GQD KPN NQD+HR AL 
Sbjct: 192 EIGFDGWRFDFVKGYAPSISKIYMEQTKPDFAVGEKWDSISYGQDGKPNYNQDSHRGALV 251

Query: 224 NWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTG 283
           NWV ++G  +TAFDFTTKGILQAAVQGELWRL D NGKPPG IG+KPE+AVTFIDNHDTG
Sbjct: 252 NWVESAGGAITAFDFTTKGILQAAVQGELWRLIDPNGKPPGMIGVKPENAVTFIDNHDTG 311

Query: 284 STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTST 343
           STQR+WPFPSDKV+QGYAYILTHPGTPSIFYDHF DWGLKE+I KLS+IR RNGI + ST
Sbjct: 312 STQRLWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEQIAKLSSIRLRNGINEKST 371

Query: 344 VNIMASEGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAVWE 394
           V IMASEGDLYVA +D KI++KIG   +LGNLIPS+  +ATSG +YAVWE
Sbjct: 372 VKIMASEGDLYVAKIDNKIMVKIGPKMDLGNLIPSNLHVATSGQDYAVWE 421

[Close]

BLAST of HG10014122 vs. ExPASy Swiss-Prot
Match: P04063 (Alpha-amylase type B isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.2 PE=1 SV=3)

HSP 1 Score: 502.7 bits (1293), Expect = 3.9e-141
Identity = 227/353 (64.31%), Postives = 276/353 (78.19%), Query Frame = 0


Query: 44  GYLPGRLYDLNASKYGTQDELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEG 103
           GY+PGRLYDL+ASKYG + +LKSLI ALH KG+KAIADIVINHRTAE +D RGI+CIFEG
Sbjct: 74  GYMPGRLYDLDASKYGNKAQLKSLIGALHGKGVKAIADIVINHRTAEHKDGRGIYCIFEG 133

Query: 104 GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKT 163
           GTPD+RLDWGP  IC DD  Y+DG GNPD+G  +  APDIDHLN +VQKE+ EW+ WLK 
Sbjct: 134 GTPDARLDWGPHMICRDDRPYADGTGNPDTGADFGAAPDIDHLNLRVQKELVEWLNWLKA 193

Query: 164 EIGFDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALA 223
           +IGFDGWRFDF KGY+  + K+Y+D++ P FAV E W SL+ G D KPN NQD HR  L 
Sbjct: 194 DIGFDGWRFDFAKGYSADVAKIYIDRSEPSFAVAEIWTSLAYGGDGKPNLNQDQHRQELV 253

Query: 224 NWVRASG--DTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHD 283
           NWV   G     T FDFTTKGIL  AV+GELWRL+ ++GK PG IG  P  AVTF+DNHD
Sbjct: 254 NWVDKVGGKGPATTFDFTTKGILNVAVEGELWRLRGTDGKAPGMIGWWPAKAVTFVDNHD 313

Query: 284 TGSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKT 343
           TGSTQ MWPFPSD+V+QGYAYILTHPGTP IFYDHF DWGLKEEI +L ++R R+GI   
Sbjct: 314 TGSTQHMWPFPSDRVMQGYAYILTHPGTPCIFYDHFFDWGLKEEIDRLVSVRTRHGIHNE 373

Query: 344 STVNIMASEGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 395
           S + I+ ++ DLY+A +DGK+I+K+G   ++GNLIP  +++A  G +YAVWE+
Sbjct: 374 SKLQIIEADADLYLAEIDGKVIVKLGPRYDVGNLIPGGFKVAAHGNDYAVWEK 426

[Close]

BLAST of HG10014122 vs. ExPASy Swiss-Prot
Match: P27934 (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 5.6e-140
Identity = 218/351 (62.11%), Postives = 272/351 (77.49%), Query Frame = 0


Query: 44  GYLPGRLYDLNASKYGTQDELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEG 103
           GY+PGRLYDL+ASKYGT+ ELKSLI A H K ++ +ADIVINHR A+ +D RG++C+FEG
Sbjct: 75  GYMPGRLYDLDASKYGTEAELKSLIEAFHDKNVECLADIVINHRCADYKDSRGVYCVFEG 134

Query: 104 GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKT 163
           GTPD RLDWGP  ICSDDT+YS+G+G+ D+G  +  APDIDHLNP+VQ+E+++W+ WL+T
Sbjct: 135 GTPDGRLDWGPDMICSDDTQYSNGRGHRDTGAGFGAAPDIDHLNPRVQRELTDWLNWLRT 194

Query: 164 EIGFDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALA 223
           ++GFDGWR DF KGY+  + ++Y+D T P F VGE W SL    D KP+ NQDA R  L 
Sbjct: 195 DLGFDGWRLDFAKGYSAPLARIYVDNTNPTFVVGEIWSSLIYNGDGKPSTNQDADRQELV 254

Query: 224 NWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTG 283
           NWV   G   TAFDFTTKGILQAAVQGELWRL D NGK PG +G  P+ AVTF+DNHDTG
Sbjct: 255 NWVEGVGKPATAFDFTTKGILQAAVQGELWRLHDGNGKAPGLMGWMPDQAVTFVDNHDTG 314

Query: 284 STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTST 343
           STQ +WPFPSDKV+QGYAYILTHPG P IFYDH  DW L+ EI  L+ IR+RNGI   ST
Sbjct: 315 STQSLWPFPSDKVMQGYAYILTHPGIPCIFYDHVFDWNLQHEIATLAEIRSRNGIHAEST 374

Query: 344 VNIMASEGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 395
           ++I+ +EGD+YVA +DGK+I K+G   + G +IPSD+ +   G +Y VWE+
Sbjct: 375 LDILKAEGDIYVAMIDGKVITKLGPRYDAGGIIPSDFHVVAHGNDYCVWEK 425

[Close]

BLAST of HG10014122 vs. ExPASy Swiss-Prot
Match: P27939 (Alpha-amylase isozyme 3C OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.7 PE=2 SV=2)

HSP 1 Score: 496.5 bits (1277), Expect = 2.8e-139
Identity = 222/352 (63.07%), Postives = 272/352 (77.27%), Query Frame = 0


Query: 44  GYLPGRLYDLNASKYGTQDELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEG 103
           GY+PGRLYDL+ASKYGT  EL+SLIAA H K IK +ADIVINHR A+ +D RGI+CIFEG
Sbjct: 76  GYMPGRLYDLDASKYGTGAELRSLIAAFHSKSIKCVADIVINHRCADYKDSRGIYCIFEG 135

Query: 104 GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKT 163
           GTPDSRLDWGP  ICSDDT+YS+G+G+ D+G  +  APDIDHLN +VQ E+S+W+ WLK+
Sbjct: 136 GTPDSRLDWGPDMICSDDTQYSNGRGHRDTGADFGAAPDIDHLNTRVQTELSDWLNWLKS 195

Query: 164 EIGFDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALA 223
           ++GFDGWR DF KGY+ ++ K Y+D T P F V E W ++    + +P+ NQD  R  L 
Sbjct: 196 DVGFDGWRLDFAKGYSATVAKTYVDNTDPSFVVAEIWSNMRYDGNGEPSWNQDGDRQELV 255

Query: 224 NWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTG 283
           NW +A G   +AFDFTTKG LQAAVQGELWR+KD NGK PG IG  PE AVTFIDNHDTG
Sbjct: 256 NWAQAVGGPASAFDFTTKGELQAAVQGELWRMKDGNGKAPGMIGWLPEKAVTFIDNHDTG 315

Query: 284 STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTST 343
           STQ  WPFPSDKV+QGYAYILTHPG P IFYDH  DW LK+EI  L+A+R+RNGI   S 
Sbjct: 316 STQNSWPFPSDKVMQGYAYILTHPGVPCIFYDHVFDWNLKQEISTLAAVRSRNGIHPGSK 375

Query: 344 VNIMASEGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAVWERN 396
           +NI+A++GD+YVA +D K+I KIG   ++GNLIPSD+ +   G  Y VWE++
Sbjct: 376 LNILAADGDVYVAMIDDKVITKIGTRYDVGNLIPSDFHVVAHGNNYCVWEKS 427

[Close]

BLAST of HG10014122 vs. ExPASy Swiss-Prot
Match: P27933 (Alpha-amylase isozyme 3D OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.3 PE=2 SV=2)

HSP 1 Score: 496.5 bits (1277), Expect = 2.8e-139
Identity = 219/351 (62.39%), Postives = 269/351 (76.64%), Query Frame = 0


Query: 44  GYLPGRLYDLNASKYGTQDELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEG 103
           GY+PGRLYDL+ASKYGT  ELKSLIAA H KG++ +AD+VINHR AEK+D RG++C+FEG
Sbjct: 75  GYMPGRLYDLDASKYGTAAELKSLIAAFHGKGVQCVADVVINHRCAEKKDARGVYCVFEG 134

Query: 104 GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKT 163
           GTPD RLDWGP  ICSDDT+YSDG G+ D+GE +  APDIDHLNP+VQ+E+++W+ WLK+
Sbjct: 135 GTPDDRLDWGPGMICSDDTQYSDGTGHRDTGEGFGAAPDIDHLNPRVQRELTDWLNWLKS 194

Query: 164 EIGFDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALA 223
           ++GFDGWR DF KGY+  I K+Y++   P F V E W+SLS   D KP  NQD  R  L 
Sbjct: 195 DVGFDGWRLDFAKGYSTDIAKMYVESCKPGFVVAEIWNSLSYNGDGKPAANQDQGRQELV 254

Query: 224 NWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTG 283
           NWV A G     FDFTTKG+LQA VQGELWRL+D NGK  G IG  PE AVTF+DNHDTG
Sbjct: 255 NWVNAVGGPAMTFDFTTKGLLQAGVQGELWRLRDGNGKAAGMIGWLPEKAVTFVDNHDTG 314

Query: 284 STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTST 343
           STQ++WPFPSDKV+QGYAYILTHPG P IFYDH  DW LK+EI  L+AIR RNGI   S 
Sbjct: 315 STQKLWPFPSDKVMQGYAYILTHPGVPCIFYDHMFDWNLKQEITALAAIRERNGINAGSK 374

Query: 344 VNIMASEGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 395
           + I+ ++ D YVA VD K+++KIG   ++GN +PSD+     G +Y+VWE+
Sbjct: 375 LRIVVADADAYVAVVDEKVMVKIGTRYDVGNAVPSDFHQTVHGKDYSVWEK 425

[Close]

BLAST of HG10014122 vs. ExPASy TrEMBL
Match: A0A6J1CMA4 (Alpha-amylase OS=Momordica charantia OX=3673 GN=LOC111012587 PE=3 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 2.8e-195
Identity = 319/351 (90.88%), Postives = 341/351 (97.15%), Query Frame = 0


Query: 44  GYLPGRLYDLNASKYGTQDELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEG 103
           GYLPGRLYDL+ASKYGTQ+ELKSLIAALH+KGIKAIADIVINHRTAEKQD+RGIWCIFEG
Sbjct: 87  GYLPGRLYDLDASKYGTQNELKSLIAALHEKGIKAIADIVINHRTAEKQDQRGIWCIFEG 146

Query: 104 GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKT 163
           GTPD+RLDWGPSFICSDDT+YSDGKGNPDSGEPYPPAPDIDHL PQVQKEISEWMRWLKT
Sbjct: 147 GTPDNRLDWGPSFICSDDTDYSDGKGNPDSGEPYPPAPDIDHLKPQVQKEISEWMRWLKT 206

Query: 164 EIGFDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALA 223
           EIGFDGWRFDFVKGYAPS+TKLYMDQT PDFAVGEKWDSLS+G D K NQNQDAHRD+LA
Sbjct: 207 EIGFDGWRFDFVKGYAPSVTKLYMDQTSPDFAVGEKWDSLSDGPDGKLNQNQDAHRDSLA 266

Query: 224 NWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTG 283
           NWV+ASGD VTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTF+DNHDTG
Sbjct: 267 NWVKASGDAVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFVDNHDTG 326

Query: 284 STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTST 343
           STQ++WPFPSDKVIQGYAYILTHPGTP+IFYDHFV+WGLKEEI++LS IRARN IGKTS 
Sbjct: 327 STQKLWPFPSDKVIQGYAYILTHPGTPAIFYDHFVEWGLKEEIIRLSEIRARNRIGKTSA 386

Query: 344 VNIMASEGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 395
           VNI+AS+ DLYVAGVDGKIIMKIGANTNLGNLIPS++QLATSGMEYAVWE+
Sbjct: 387 VNILASDADLYVAGVDGKIIMKIGANTNLGNLIPSEFQLATSGMEYAVWEK 437

[Close]

BLAST of HG10014122 vs. ExPASy TrEMBL
Match: A0A6J1IGI6 (Alpha-amylase OS=Cucurbita maxima OX=3661 GN=LOC111476734 PE=3 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 4.1e-194
Identity = 318/351 (90.60%), Postives = 334/351 (95.16%), Query Frame = 0


Query: 44  GYLPGRLYDLNASKYGTQDELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEG 103
           GYLPGRLYDLNASKYGTQDELKSLIAALH  GIKA+ADIVINHRTA+KQDERGIWCIFEG
Sbjct: 74  GYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTADKQDERGIWCIFEG 133

Query: 104 GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKT 163
           GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPY  APDIDHLNPQVQ E+SEWMRWLKT
Sbjct: 134 GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYSAAPDIDHLNPQVQNELSEWMRWLKT 193

Query: 164 EIGFDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALA 223
           EIGFDGWRFDFVKGYAP ITKLYMDQT PDFAVGEKWDSLS G D KP+ NQD HR+ALA
Sbjct: 194 EIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSPGPDGKPDPNQDGHREALA 253

Query: 224 NWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTG 283
           NWVR SGDTVTAFDFTTKGILQAAVQGELWRLKD NGKPPGFIGIKPESAVTFIDNHDTG
Sbjct: 254 NWVRDSGDTVTAFDFTTKGILQAAVQGELWRLKDPNGKPPGFIGIKPESAVTFIDNHDTG 313

Query: 284 STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTST 343
           STQ++WPFPSDKVIQGYAYILTHPGTPSIFYDHFV+WGLKEE++KLSAIR RNGIG+ ST
Sbjct: 314 STQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLSAIRTRNGIGERST 373

Query: 344 VNIMASEGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 395
           VNIMAS+GDLYVAG+DGKIIMKIG NTNLGNLIPSDY+LATSGM+YAVWE+
Sbjct: 374 VNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLIPSDYELATSGMQYAVWEK 424

[Close]

BLAST of HG10014122 vs. ExPASy TrEMBL
Match: A0A6J1FCS4 (Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111442931 PE=3 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 5.3e-194
Identity = 317/351 (90.31%), Postives = 335/351 (95.44%), Query Frame = 0


Query: 44  GYLPGRLYDLNASKYGTQDELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEG 103
           GYLPGRLYDLNASKYGTQ+ELKSLIAALH  GIKA+ADIVINHRTA+KQDERGIWCIFEG
Sbjct: 98  GYLPGRLYDLNASKYGTQNELKSLIAALHNNGIKAVADIVINHRTADKQDERGIWCIFEG 157

Query: 104 GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKT 163
           GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPY  APDIDHLNPQVQ E+SEWMRWLKT
Sbjct: 158 GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYSAAPDIDHLNPQVQNELSEWMRWLKT 217

Query: 164 EIGFDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALA 223
           EIGFDGWRFDFVKGYAP ITKLYMDQT PDFAVGEKWDSLS G D KP+ NQD HR+ALA
Sbjct: 218 EIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGKPDPNQDGHREALA 277

Query: 224 NWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTG 283
           NWVR SGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTG
Sbjct: 278 NWVRDSGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTG 337

Query: 284 STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTST 343
           STQ++WPFPSDKVIQGYAYILTHPGTPSIFYDHFV+WGLKEE++KLSAIRARNGIG+ ST
Sbjct: 338 STQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLSAIRARNGIGERST 397

Query: 344 VNIMASEGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 395
           VNIMAS+GDLYVAG+DGKIIMKIG NTNLGNL+PSDY+LA SGM+YAVWE+
Sbjct: 398 VNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYAVWEK 448

[Close]

BLAST of HG10014122 vs. ExPASy TrEMBL
Match: A0A6A1W532 (Alpha-amylase OS=Morella rubra OX=262757 GN=CJ030_MR3G003095 PE=3 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 8.0e-174
Identity = 284/351 (80.91%), Postives = 316/351 (90.03%), Query Frame = 0


Query: 44  GYLPGRLYDLNASKYGTQDELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEG 103
           GY+PGRLYDL+ASKYG++DELKSLI+ALH+KGIKAIADIVINHRTAEKQD RGIWCIFEG
Sbjct: 102 GYMPGRLYDLDASKYGSKDELKSLISALHEKGIKAIADIVINHRTAEKQDGRGIWCIFEG 161

Query: 104 GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKT 163
           GTPD+RLDWGPSFIC DDT+YSDG GN DSGE + PAPDIDHLNPQVQKE+S+WM WLKT
Sbjct: 162 GTPDARLDWGPSFICKDDTDYSDGSGNLDSGEDFRPAPDIDHLNPQVQKELSDWMNWLKT 221

Query: 164 EIGFDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALA 223
           EIGF GWRFDFVKGYAPSITK+YM+QT PDFAVGE W+SL+     KPN NQDAHR ALA
Sbjct: 222 EIGFSGWRFDFVKGYAPSITKIYMEQTTPDFAVGENWNSLAYDDGGKPNPNQDAHRGALA 281

Query: 224 NWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTG 283
           NWV+ +G  VTAFDFTTKGILQAAV+GELWRLKDSNGKPPG IG KPESAVTFIDNHDTG
Sbjct: 282 NWVQGAGGAVTAFDFTTKGILQAAVEGELWRLKDSNGKPPGLIGTKPESAVTFIDNHDTG 341

Query: 284 STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTST 343
           STQ++WPFPSDKV+QGYAYILTHPG P+IFYDHF DWGLKEEI KLSAIR+RNGI  TS 
Sbjct: 342 STQKLWPFPSDKVMQGYAYILTHPGIPTIFYDHFFDWGLKEEISKLSAIRSRNGISSTSR 401

Query: 344 VNIMASEGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 395
           VNI+AS+ DLY+A VD KIIMKIG   +LGNL+PS+YQ+ATSG +YAVWE+
Sbjct: 402 VNILASDADLYLAEVDNKIIMKIGPKMDLGNLVPSNYQVATSGNDYAVWEK 452

[Close]

BLAST of HG10014122 vs. ExPASy TrEMBL
Match: A0A2I4EXA7 (Alpha-amylase OS=Juglans regia OX=51240 GN=LOC108993530 PE=3 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 2.0e-172
Identity = 282/351 (80.34%), Postives = 314/351 (89.46%), Query Frame = 0


Query: 44  GYLPGRLYDLNASKYGTQDELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEG 103
           GYLPGRLYDL+ SKYG++DELK+LIAALH+KGIK +ADIVINHRTAEKQD+RGIW IFEG
Sbjct: 72  GYLPGRLYDLDVSKYGSKDELKALIAALHEKGIKVVADIVINHRTAEKQDDRGIWSIFEG 131

Query: 104 GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKT 163
           GTPD+RLDWGPSFIC DD  YSDG GNPDSGE Y PAPDIDH NPQVQKE+SEWM WLKT
Sbjct: 132 GTPDARLDWGPSFICKDDIAYSDGTGNPDSGEGYDPAPDIDHRNPQVQKELSEWMNWLKT 191

Query: 164 EIGFDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALA 223
           EIG+DGWRFDFVKGYAPSI K YM+QT PDFAVGE W+SL+ G D KPN NQDAHR ALA
Sbjct: 192 EIGYDGWRFDFVKGYAPSIMKNYMEQTKPDFAVGENWNSLAYGDDGKPNPNQDAHRGALA 251

Query: 224 NWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTG 283
            WV ++G  VTAFDFTTKGILQAAV+GELWRLKDSNGKPPG IGIKPESAVTFIDNHDTG
Sbjct: 252 EWVGSAGGAVTAFDFTTKGILQAAVEGELWRLKDSNGKPPGLIGIKPESAVTFIDNHDTG 311

Query: 284 STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTST 343
           STQ +WPFPSDKV+QGYAYILTHPGTP+IFYDHF DWGLKEEI KL+AIR+RNGI   S 
Sbjct: 312 STQNIWPFPSDKVMQGYAYILTHPGTPTIFYDHFFDWGLKEEISKLTAIRSRNGINTRSI 371

Query: 344 VNIMASEGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 395
           V+I+AS+GDLYVAG+D KIIMKIG   +LGNLIPS++Q++TSG +YAVWE+
Sbjct: 372 VSILASDGDLYVAGIDDKIIMKIGPKMDLGNLIPSNFQVSTSGKDYAVWEK 422

[Close]

BLAST of HG10014122 vs. TAIR 10
Match: AT4G25000.1 (alpha-amylase-like )

HSP 1 Score: 495.7 bits (1275), Expect = 3.4e-140
Identity = 231/352 (65.62%), Postives = 282/352 (80.11%), Query Frame = 0


Query: 44  GYLPGRLYDLNASKYGTQDELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEG 103
           GYLPG+LYDLN+SKYG++ ELKSLI AL+QKGIKA+ADIVINHRTAE++D++  +C FEG
Sbjct: 75  GYLPGKLYDLNSSKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEG 134

Query: 104 GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKT 163
           GT D RLDW PSF+C +D ++  G GN D+G  +  APDIDHLNP+VQKE+SEWM WLKT
Sbjct: 135 GTSDDRLDWDPSFVCRNDPKF-PGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKT 194

Query: 164 EIGFDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALA 223
           EIGF GWRFD+V+GYA SITKLY+  T PDFAVGEKWD +  G D K + +Q+ HR  L 
Sbjct: 195 EIGFHGWRFDYVRGYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLK 254

Query: 224 NWV-RASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDT 283
            W+  A G  +TAFDFTTKGILQ+AV+GELWRLKDS GKPPG IGI P +AVTFIDNHD 
Sbjct: 255 QWIEEAGGGVLTAFDFTTKGILQSAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHD- 314

Query: 284 GSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSAIRARNGIGKTS 343
             T R W FPSDKV+ GY YILTHPGTP IFY+H+++WGLKE I KL AIR +NGIG TS
Sbjct: 315 --TFRTWVFPSDKVLLGYVYILTHPGTPCIFYNHYIEWGLKESISKLVAIRNKNGIGSTS 374

Query: 344 TVNIMASEGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAVWER 395
           +V I A+E DLY+A +D K+IMKIG   ++G L+PS++ LA SG+++AVWE+
Sbjct: 375 SVTIKAAEADLYLAMIDDKVIMKIGPKQDVGTLVPSNFALAYSGLDFAVWEK 422

[Close]

BLAST of HG10014122 vs. TAIR 10
Match: AT1G69830.1 (alpha-amylase-like 3 )

HSP 1 Score: 327.0 bits (837), Expect = 2.1e-89
Identity = 167/352 (47.44%), Postives = 220/352 (62.50%), Query Frame = 0


Query: 44  GYLPGRLYDLNASKYGTQDELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWCIFEG 103
           GY+P  LY+LN S+YGT DELK  +   H+ GIK + D V+NHR A  +++ G+W +F G
Sbjct: 544 GYMPKDLYNLN-SRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG 603

Query: 104 GTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMRWLKT 163
                RL+W    + +DD  +  G+GN  SG+ +  AP+IDH    V+K+I EW+ W+  
Sbjct: 604 -----RLNWDDRAVVADDPHF-QGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMME 663

Query: 164 EIGFDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHRDALA 223
           E+G+DGWR DFV+G+     K YMD + P FAVGE WDSLS     + + NQDAHR  + 
Sbjct: 664 EVGYDGWRLDFVRGFWGGYVKDYMDASKPYFAVGEYWDSLSYTYG-EMDYNQDAHRQRIV 723

Query: 224 NWVRASGDTVTAFDFTTKGILQAAVQG-ELWRLKDSNGKPPGFIGIKPESAVTFIDNHDT 283
           +W+ A+     AFD TTKGIL  A+Q  E WRL D  GKPPG +G  P  AVTFI+NHDT
Sbjct: 724 DWINATSGAAGAFDVTTKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDT 783

Query: 284 GSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDH-FVDWGLKEEIMKLSAIRARNGIGKT 343
           GSTQ  W FP  K +QGYAYILTHPGTP++F+DH F D+    EI  L ++R R  +   
Sbjct: 784 GSTQGHWRFPEGKEMQGYAYILTHPGTPAVFFDHIFSDY--HSEIAALLSLRNRQKLHCR 843

Query: 344 STVNIMASEGDLYVAGVDGKIIMKIGANTNLGNLIPSDYQLATSGMEYAVWE 394
           S VNI  SE D+Y A +D K+ MKIG           ++ +A  G +Y VWE
Sbjct: 844 SEVNIDKSERDVYAAIIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885

[Close]

BLAST of HG10014122 vs. TAIR 10
Match: AT1G76130.1 (alpha-amylase-like 2 )

HSP 1 Score: 323.6 bits (828), Expect = 2.3e-88
Identity = 167/359 (46.52%), Postives = 224/359 (62.40%), Query Frame = 0


Query: 40  LTVSGYLPGRLYDLNASKYGTQDELKSLIAALHQKGIKAIADIVINHRTAEKQDERGIWC 99
           L   GYLP  LY LN S YG++  LKSL+  + Q  ++A+ADIVINHR    +   G++ 
Sbjct: 69  LAPEGYLPQDLYSLN-SAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYN 128

Query: 100 IFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKEISEWMR 159
            ++G      L W    +    T  + G GN  +G+ +   P++DH    V+K+I  W+R
Sbjct: 129 RYDG----ISLPWDEHAV----TSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLR 188

Query: 160 WLKTEIGFDGWRFDFVKGYAPSITKLYMDQTLPDFAVGEKWDSLSEGQDRKPNQNQDAHR 219
           WL+  +GF  +RFDF +GY+ +  K Y+    P F+VGE WDS +       + NQD+HR
Sbjct: 189 WLRNTVGFQDFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNY-NGHGLDYNQDSHR 248

Query: 220 DALANWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDN 279
             + +W+ A+G    AFDFTTKGILQ AV+G+ WRL D+ GKPPG +G  P  AVTF+DN
Sbjct: 249 QRIISWIDATGQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDN 308

Query: 280 HDTGSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWG--LKEEIMKLSAIRARNG 339
           HDTGSTQ  WPFPS  V++GYAYILTHPG P +FYDHF DWG  + ++I+KL  IR R  
Sbjct: 309 HDTGSTQAHWPFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQD 368

Query: 340 IGKTSTVNIMASEGDLYVAGVDGKIIMKIGANTNLGNLIPS--DYQLATSGMEYAVWER 395
           I   STV ++ +E +LY A V  KI MK+G     G+  PS  D+ LATSG  YAVW +
Sbjct: 369 IHSRSTVRVLKAESNLYAAIVGEKICMKLGD----GSWCPSGRDWTLATSGHRYAVWHK 413

The following BLAST results are available for this feature:

BLAST of HG10014122 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896126.1	2.1e-200	94.30	alpha-amylase-like [Benincasa hispida]	[more]
XP_022142476.1	5.8e-195	90.88	alpha-amylase [Momordica charantia]	[more]
XP_023536126.1	4.9e-194	90.88	alpha-amylase, partial [Cucurbita pepo subsp. pepo]	[more]
XP_022976296.1	8.4e-194	90.60	alpha-amylase [Cucurbita maxima]	[more]
XP_022936258.1	1.1e-193	90.31	alpha-amylase, partial [Cucurbita moschata]	[more]

BLAST of HG10014122 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17859	5.0e-173	79.71	Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1	[more]
P04063	3.9e-141	64.31	Alpha-amylase type B isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.2 PE=1 SV=3	[more]
P27934	5.6e-140	62.11	Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2...	[more]
P27939	2.8e-139	63.07	Alpha-amylase isozyme 3C OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.7 PE=2...	[more]
P27933	2.8e-139	62.39	Alpha-amylase isozyme 3D OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.3 PE=2...	[more]

BLAST of HG10014122 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CMA4	2.8e-195	90.88	Alpha-amylase OS=Momordica charantia OX=3673 GN=LOC111012587 PE=3 SV=1	[more]
A0A6J1IGI6	4.1e-194	90.60	Alpha-amylase OS=Cucurbita maxima OX=3661 GN=LOC111476734 PE=3 SV=1	[more]
A0A6J1FCS4	5.3e-194	90.31	Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111442931 PE=3 SV=1	[more]
A0A6A1W532	8.0e-174	80.91	Alpha-amylase OS=Morella rubra OX=262757 GN=CJ030_MR3G003095 PE=3 SV=1	[more]
A0A2I4EXA7	2.0e-172	80.34	Alpha-amylase OS=Juglans regia OX=51240 GN=LOC108993530 PE=3 SV=1	[more]

BLAST of HG10014122 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25000.1	3.4e-140	65.63	alpha-amylase-like	[more]
AT1G69830.1	2.1e-89	47.44	alpha-amylase-like 3	[more]
AT1G76130.1	2.3e-88	46.52	alpha-amylase-like 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006046	Alpha amylase	PRINTS	PR00110	ALPHAAMYLASE	coord: 75..86 score: 49.51 coord: 271..283 score: 51.43 coord: 167..178 score: 40.85
IPR012850	Alpha-amylase, C-terminal beta-sheet	SMART	SM00810	alpha-amyl_c2	coord: 334..394 e-value: 4.8E-29 score: 112.5
IPR012850	Alpha-amylase, C-terminal beta-sheet	PFAM	PF07821	Alpha-amyl_C2	coord: 335..393 e-value: 4.4E-17 score: 62.1
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	SMART	SM00642	aamy	coord: 24..333 e-value: 3.4E-29 score: 113.0
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	PFAM	PF00128	Alpha-amylase	coord: 56..312 e-value: 3.5E-13 score: 49.8
IPR013775	Alpha-amylase, plant	PIRSF	PIRSF001028	Alpha-amylase_plant	coord: 38..395 e-value: 1.2E-190 score: 631.8
IPR013780	Glycosyl hydrolase, all-beta	GENE3D	2.60.40.1180		coord: 338..395 e-value: 2.8E-22 score: 80.6
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 33..337 e-value: 8.1E-124 score: 415.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 123..144
None	No IPR available	PANTHER	PTHR43447	ALPHA-AMYLASE	coord: 42..394
None	No IPR available	PANTHER	PTHR43447:SF37	ALPHA-AMYLASE 1	coord: 42..394
None	No IPR available	CDD	cd11314	AmyAc_arch_bac_plant_AmyA	coord: 44..344 e-value: 3.97609E-144 score: 409.688
None	No IPR available	SUPERFAMILY	51011	Glycosyl hydrolase domain	coord: 338..394
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 39..323

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10014122.1	HG10014122.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004556	alpha-amylase activity
molecular_function	GO:0103025	alpha-amylase activity (releasing maltohexaose)
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0043169	cation binding