HG10011995 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10011995
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Trypsin inhibitor
Location	Chr01: 16347055 .. 16347397 (+)
RNA-Seq Expression	HG10011995
Synteny	HG10011995

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGAAGAAATTGTTCTTCCTTCAGGTTAGTAAGACTCCTCCTCTTCCCACTTGGGAATTTCCAAATAGTTCTTCTTTGTCCCTTTAATTTTTCTTAATATTTTGAATTGAGTATTTACTTTTTTATCACCATTCAATTTATTTAGGGAAATCCTCATGGCCCGAACTCGTATTCATCGAATCTTCCACTGCAGTACGTCTTATAGAGAGAGATAATCCTCATGTGAAGGCAGTTGTACTATTAGCTGGATCTCCAGTTACTGAAGATTTTAGACTAGATCGAGTTCGAGTTTTTGTTAACATAAATAATGTGGTGGTTAGTGTTCCTACGGTTGGTTGA

mRNA sequence

ATGGCTGAAGAAATTGTTCTTCCTTCAGGGAAATCCTCATGGCCCGAACTCGTATTCATCGAATCTTCCACTGCAGTACGTCTTATAGAGAGAGATAATCCTCATGTGAAGGCAGTTGTACTATTAGCTGGATCTCCAGTTACTGAAGATTTTAGACTAGATCGAGTTCGAGTTTTTGTTAACATAAATAATGTGGTGGTTAGTGTTCCTACGGTTGGTTGA

Coding sequence (CDS)

ATGGCTGAAGAAATTGTTCTTCCTTCAGGGAAATCCTCATGGCCCGAACTCGTATTCATCGAATCTTCCACTGCAGTACGTCTTATAGAGAGAGATAATCCTCATGTGAAGGCAGTTGTACTATTAGCTGGATCTCCAGTTACTGAAGATTTTAGACTAGATCGAGTTCGAGTTTTTGTTAACATAAATAATGTGGTGGTTAGTGTTCCTACGGTTGGTTGA

Protein sequence

MAEEIVLPSGKSSWPELVFIESSTAVRLIERDNPHVKAVVLLAGSPVTEDFRLDRVRVFVNINNVVVSVPTVG

Homology

[Close]

BLAST of HG10011995 vs. NCBI nr
Match: XP_011654473.1 (inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetical protein Csa_018430 [Cucumis sativus])

HSP 1 Score: 98.6 bits (244), Expect = 2.4e-17
Identity = 54/75 (72.00%), Postives = 60/75 (80.00%), Query Frame = 0


Query: 1  MAEE-IVLP-SGKSSWPELVFIESSTAVRLIERDNPHVKAVVLLAGSPVTEDFRLDRVRV 60
          MAEE IV P SGKSSWPELVF++SS AV  IERD P VK VVLLAGSPVTED R +RVR+
Sbjct: 1  MAEEKIVFPSSGKSSWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRI 60

Query: 61 FVNINNVVVSVPTVG 74
          FVN+N+ VV VP  G
Sbjct: 61 FVNMNDRVVEVPRTG 75

[Close]

BLAST of HG10011995 vs. NCBI nr
Match: KAA8527727.1 (hypothetical protein F0562_035404 [Nyssa sinensis])

HSP 1 Score: 84.0 bits (206), Expect = 6.0e-13
Identity = 39/64 (60.94%), Postives = 48/64 (75.00%), Query Frame = 0


Query: 10 GKSSWPELVFIESSTAVRLIERDNPHVKAVVLLAGSPVTEDFRLDRVRVFVNINNVVVSV 69
          GKSSWPELV ++  TA   IER+NP V A+++L GSPVT DFR DRVRV+VN N +V  V
Sbjct: 7  GKSSWPELVGVQGETATATIERENPLVTALIVLEGSPVTPDFRCDRVRVYVNENGIVTLV 66

Query: 70 PTVG 74
          P +G
Sbjct: 67 PAIG 70

[Close]

BLAST of HG10011995 vs. NCBI nr
Match: XP_026426364.1 (inhibitor of trypsin and hageman factor-like [Papaver somniferum] >RZC83602.1 hypothetical protein C5167_046390 [Papaver somniferum])

HSP 1 Score: 81.3 bits (199), Expect = 3.9e-12
Identity = 40/64 (62.50%), Postives = 45/64 (70.31%), Query Frame = 0


Query: 10 GKSSWPELVFIESSTAVRLIERDNPHVKAVVLLAGSPVTEDFRLDRVRVFVNINNVVVSV 69
          GKSSWPELV     TA R+IER NP+V A+VLL G+P T DFR DRV VFVN    VV  
Sbjct: 16 GKSSWPELVGFNGGTAARIIERQNPNVNAIVLLEGTPTTLDFRCDRVWVFVNEAGKVVQT 75

Query: 70 PTVG 74
          PT+G
Sbjct: 76 PTIG 79

[Close]

BLAST of HG10011995 vs. NCBI nr
Match: KAA0049292.1 (trypsin inhibitor [Cucumis melo var. makuwa] >TYK17266.1 trypsin inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 80.5 bits (197), Expect = 6.7e-12
Identity = 38/64 (59.38%), Postives = 50/64 (78.12%), Query Frame = 0


Query: 10 GKSSWPELVFIESSTAVRLIERDNPHVKAVVLLAGSPVTEDFRLDRVRVFVNINNVVVSV 69
          GK SWP+LV +  S A  +IER+NP+V+AVVL  G+PVT+DFR +RVRV+VN N +V S 
Sbjct: 6  GKRSWPQLVGVGGSVAKAIIERENPNVRAVVLEVGTPVTKDFRCNRVRVWVNKNGLVASP 65

Query: 70 PTVG 74
          PT+G
Sbjct: 66 PTIG 69

[Close]

BLAST of HG10011995 vs. NCBI nr
Match: KGN50840.1 (hypothetical protein Csa_004718 [Cucumis sativus])

HSP 1 Score: 80.1 bits (196), Expect = 8.7e-12
Identity = 40/69 (57.97%), Postives = 54/69 (78.26%), Query Frame = 0


Query: 6  VLPSGKSSWPELVFIESSTAVRLIERDNPHVK-AVVLLAGSPVTEDFRLDRVRVFVNINN 65
          + P GK+ WPELV ++++TA  +I++DNP+V+  VVLLAGS  TED R DRV VFVNI++
Sbjct: 4  ICPPGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVNIHD 63

Query: 66 VVVSVPTVG 74
          +VV VP VG
Sbjct: 64 LVVEVPKVG 72

[Close]

BLAST of HG10011995 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 5.7e-14
Identity = 37/64 (57.81%), Postives = 48/64 (75.00%), Query Frame = 0


Query: 10 GKSSWPELVFIESSTAVRLIERDNPHVKAVVLLAGSPVTEDFRLDRVRVFVNINNVVVSV 69
          GKSSWP LV +  S A  +IER NP+VKAV+L  G+PVT+DFR +RVR++VN   +VVS 
Sbjct: 5  GKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVVSP 64

Query: 70 PTVG 74
          P +G
Sbjct: 65 PRIG 68

[Close]

BLAST of HG10011995 vs. ExPASy Swiss-Prot
Match: P24076 (Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 9.1e-12
Identity = 33/64 (51.56%), Postives = 43/64 (67.19%), Query Frame = 0


Query: 10 GKSSWPELVFIESSTAVRLIERDNPHVKAVVLLAGSPVTEDFRLDRVRVFVNINNVVVSV 69
          GK SWP+LV    + A  +IER+NP V+AV++  GSPVT DFR DRVRV+V    +V   
Sbjct: 5  GKRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWVTERGIVARP 64

Query: 70 PTVG 74
          P +G
Sbjct: 65 PAIG 68

[Close]

BLAST of HG10011995 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.3e-10
Identity = 33/64 (51.56%), Postives = 45/64 (70.31%), Query Frame = 0


Query: 10  GKSSWPELVFIESSTAVRLIERDNPHVKAV-VLLAGSPVTEDFRLDRVRVFVNINNVVVS 69
           GK  WPEL+ + +  A  +IE++NP +  + +LL GSPVT+DFR DRVR+FVNI   VV 
Sbjct: 47  GKQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQ 106

Query: 70  VPTV 73
           +P V
Sbjct: 107 IPRV 110

[Close]

BLAST of HG10011995 vs. ExPASy Swiss-Prot
Match: Q6XNP7 (Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2)

HSP 1 Score: 65.9 bits (159), Expect = 2.2e-10
Identity = 30/63 (47.62%), Postives = 42/63 (66.67%), Query Frame = 0


Query: 11 KSSWPELVFIESSTAVRLIERDNPHVKAVVLLAGSPVTEDFRLDRVRVFVNINNVVVSVP 70
          K+SWPELV      A  +I+ +N +VKA+V+  G P+T+D   +RVRVFV+ N VV  VP
Sbjct: 8  KNSWPELVGTNGDIAAGIIQTENANVKAIVVKEGLPITQDLNFNRVRVFVDENRVVTQVP 67

Query: 71 TVG 74
           +G
Sbjct: 68 AIG 70

[Close]

BLAST of HG10011995 vs. ExPASy Swiss-Prot
Match: P82381 (Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 6.5e-10
Identity = 32/63 (50.79%), Postives = 43/63 (68.25%), Query Frame = 0


Query: 10 GKSSWPELVFIESSTAVRLIERDNPHVKAVVLLAGSPVTEDFRLDRVRVFVNINNVVVSV 69
          GK++WPELV    + A   +ER+N +V A+VL  GS +T+DFR DRV V VN + VV SV
Sbjct: 6  GKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDHGVVTSV 65

Query: 70 PTV 73
          P +
Sbjct: 66 PHI 68

[Close]

BLAST of HG10011995 vs. ExPASy TrEMBL
Match: A0A0A0KPI0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.1e-17
Identity = 54/75 (72.00%), Postives = 60/75 (80.00%), Query Frame = 0


Query: 1  MAEE-IVLP-SGKSSWPELVFIESSTAVRLIERDNPHVKAVVLLAGSPVTEDFRLDRVRV 60
          MAEE IV P SGKSSWPELVF++SS AV  IERD P VK VVLLAGSPVTED R +RVR+
Sbjct: 1  MAEEKIVFPSSGKSSWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRI 60

Query: 61 FVNINNVVVSVPTVG 74
          FVN+N+ VV VP  G
Sbjct: 61 FVNMNDRVVEVPRTG 75

[Close]

BLAST of HG10011995 vs. ExPASy TrEMBL
Match: A0A5J5AE65 (Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_035404 PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.9e-13
Identity = 39/64 (60.94%), Postives = 48/64 (75.00%), Query Frame = 0


Query: 10 GKSSWPELVFIESSTAVRLIERDNPHVKAVVLLAGSPVTEDFRLDRVRVFVNINNVVVSV 69
          GKSSWPELV ++  TA   IER+NP V A+++L GSPVT DFR DRVRV+VN N +V  V
Sbjct: 7  GKSSWPELVGVQGETATATIERENPLVTALIVLEGSPVTPDFRCDRVRVYVNENGIVTLV 66

Query: 70 PTVG 74
          P +G
Sbjct: 67 PAIG 70

[Close]

BLAST of HG10011995 vs. ExPASy TrEMBL
Match: A0A4Y7LFX7 (Uncharacterized protein OS=Papaver somniferum OX=3469 GN=C5167_046390 PE=3 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.9e-12
Identity = 40/64 (62.50%), Postives = 45/64 (70.31%), Query Frame = 0


Query: 10 GKSSWPELVFIESSTAVRLIERDNPHVKAVVLLAGSPVTEDFRLDRVRVFVNINNVVVSV 69
          GKSSWPELV     TA R+IER NP+V A+VLL G+P T DFR DRV VFVN    VV  
Sbjct: 16 GKSSWPELVGFNGGTAARIIERQNPNVNAIVLLEGTPTTLDFRCDRVWVFVNEAGKVVQT 75

Query: 70 PTVG 74
          PT+G
Sbjct: 76 PTIG 79

[Close]

BLAST of HG10011995 vs. ExPASy TrEMBL
Match: A0A5A7U0I2 (Trypsin inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001190 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 3.2e-12
Identity = 38/64 (59.38%), Postives = 50/64 (78.12%), Query Frame = 0


Query: 10 GKSSWPELVFIESSTAVRLIERDNPHVKAVVLLAGSPVTEDFRLDRVRVFVNINNVVVSV 69
          GK SWP+LV +  S A  +IER+NP+V+AVVL  G+PVT+DFR +RVRV+VN N +V S 
Sbjct: 6  GKRSWPQLVGVGGSVAKAIIERENPNVRAVVLEVGTPVTKDFRCNRVRVWVNKNGLVASP 65

Query: 70 PTVG 74
          PT+G
Sbjct: 66 PTIG 69

[Close]

BLAST of HG10011995 vs. ExPASy TrEMBL
Match: A0A0A0KME7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 4.2e-12
Identity = 40/69 (57.97%), Postives = 54/69 (78.26%), Query Frame = 0


Query: 6  VLPSGKSSWPELVFIESSTAVRLIERDNPHVK-AVVLLAGSPVTEDFRLDRVRVFVNINN 65
          + P GK+ WPELV ++++TA  +I++DNP+V+  VVLLAGS  TED R DRV VFVNI++
Sbjct: 4  ICPPGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVNIHD 63

Query: 66 VVVSVPTVG 74
          +VV VP VG
Sbjct: 64 LVVEVPKVG 72

[Close]

BLAST of HG10011995 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 72.0 bits (175), Expect = 2.2e-13
Identity = 36/63 (57.14%), Postives = 42/63 (66.67%), Query Frame = 0


Query: 11 KSSWPELVFIESSTAVRLIERDNPHVKAVVLLAGSPVTEDFRLDRVRVFVNINNVVVSVP 70
          K+SWPEL       A  +IER+NP V A V+L GSPVT DFR DRVRVFV+ N +VV  P
Sbjct: 8  KNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVVKTP 67

Query: 71 TVG 74
            G
Sbjct: 68 KSG 70

[Close]

BLAST of HG10011995 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 63.2 bits (152), Expect = 1.0e-10
Identity = 31/64 (48.44%), Postives = 43/64 (67.19%), Query Frame = 0


Query: 10 GKSSWPELVFIESSTAVRLIERDNPHVKAVVLLAGSPVTEDFRLDRVRVFVNINNVVVSV 69
          GKSSWPEL+  +   A  +IER+NP +KAV++L G+ V E F   RV V+VN   +VV +
Sbjct: 36 GKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVNDCGIVVQI 95

Query: 70 PTVG 74
          P +G
Sbjct: 96 PIIG 99

[Close]

BLAST of HG10011995 vs. TAIR 10
Match: AT3G46860.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 48.5 bits (114), Expect = 2.6e-06
Identity = 26/63 (41.27%), Postives = 34/63 (53.97%), Query Frame = 0


Query: 11 KSSWPELVFIESSTAVRLIERDNPHVKAVVLLAGSPVTEDFRLDRVRVFVNINNVVVSVP 70
          K  WPELV      A   IER+NP+V A+VL  G    E+F  +RV V++  N  V   P
Sbjct: 23 KYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENFCCNRVFVYLGSNGQVADAP 82

Query: 71 TVG 74
           +G
Sbjct: 83 MIG 85

The following BLAST results are available for this feature:

BLAST of HG10011995 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011654473.1	2.4e-17	72.00	inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetic...	[more]
KAA8527727.1	6.0e-13	60.94	hypothetical protein F0562_035404 [Nyssa sinensis]	[more]
XP_026426364.1	3.9e-12	62.50	inhibitor of trypsin and hageman factor-like [Papaver somniferum] >RZC83602.1 hy...	[more]
KAA0049292.1	6.7e-12	59.38	trypsin inhibitor [Cucumis melo var. makuwa] >TYK17266.1 trypsin inhibitor [Cucu...	[more]
KGN50840.1	8.7e-12	57.97	hypothetical protein Csa_004718 [Cucumis sativus]	[more]

BLAST of HG10011995 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P19873	5.7e-14	57.81	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
P24076	9.1e-12	51.56	Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P16231	1.3e-10	51.56	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]
Q6XNP7	2.2e-10	47.62	Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2	[more]
P82381	6.5e-10	50.79	Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1	[more]

BLAST of HG10011995 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KPI0	1.1e-17	72.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1	[more]
A0A5J5AE65	2.9e-13	60.94	Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_035404 PE=3 SV=1	[more]
A0A4Y7LFX7	1.9e-12	62.50	Uncharacterized protein OS=Papaver somniferum OX=3469 GN=C5167_046390 PE=3 SV=1	[more]
A0A5A7U0I2	3.2e-12	59.38	Trypsin inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00...	[more]
A0A0A0KME7	4.2e-12	57.97	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1	[more]

BLAST of HG10011995 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	2.2e-13	57.14	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	1.0e-10	48.44	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT3G46860.1	2.6e-06	41.27	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 38..49 score: 46.74 coord: 50..60 score: 60.47 coord: 13..26 score: 39.75
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 11..73 e-value: 3.9E-23 score: 81.9
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 2..73
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 7..72 e-value: 2.7E-25 score: 90.4
None	No IPR available	PANTHER	PTHR33091:SF83	ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1	coord: 2..73
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 8..73

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10011995.1	HG10011995.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity