Lag0020148 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0020148
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Dof zinc finger protein 2
Location	chr5: 48430707 .. 48431243 (+)
RNA-Seq Expression	Lag0020148
Synteny	Lag0020148

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGAGGAAGAGGGAAGAAGGGCAGTGATGGAAAGAAAGGCAAAGCCAGGGAAAGATCAAATTTTGAAGTGCCCCAGATGCAATTCCAACAACACAAAGTTCTGTTATTACAACAATTACAGCCTCTCACAGCCAAGGTACTTCTGCAAGTCTTGTAGAAGATATTGGACAGCAGGTGGATCTTTGAGGAACATTCCAGTGGGTGGGGCCTCAAGGAAGAACAAAAGACCTTCAGCTAATTTTTCATCATCAGCAGCAGCAGCTGCAGCTCCTTCCAGTTCATCACCAAACAACATGAACAATAACAAGAACAGTGATAATAATAATGGTGATGATCACAGCAACAGCAACCCCATTATCATCAACAATGGCAGCTCCCAACCAAATCTCAATACTTCTTCTTCTTCTTCTTCCAATACTGCTTGTTGTTTGCAATTAATATTGGATTTCCCATTTCCATATGTAATCAGCATATTCTCTCTCCCTAGGAAGAAAAAAAATAGAACACTTTTGTGGATATTTCTAAAATTGTGA

mRNA sequence

ATGGAAGAGGAAGAGGGAAGAAGGGCAGTGATGGAAAGAAAGGCAAAGCCAGGGAAAGATCAAATTTTGAAGTGCCCCAGATGCAATTCCAACAACACAAAGTTCTGTTATTACAACAATTACAGCCTCTCACAGCCAAGGTACTTCTGCAAGTCTTGTAGAAGATATTGGACAGCAGGTGGATCTTTGAGGAACATTCCAGTGGGTGGGGCCTCAAGGAAGAACAAAAGACCTTCAGCTAATTTTTCATCATCAGCAGCAGCAGCTGCAGCTCCTTCCAGTTCATCACCAAACAACATGAACAATAACAAGAACAGTGATAATAATAATGGTGATGATCACAGCAACAGCAACCCCATTATCATCAACAATGGCAGCTCCCAACCAAATCTCAATACTTCTTCTTCTTCTTCTTCCAATACTGCTTGTTGTTTGCAATTAATATTGGATTTCCCATTTCCATATGTAATCAGCATATTCTCTCTCCCTAGGAAGAAAAAAAATAGAACACTTTTGTGGATATTTCTAAAATTGTGA

Coding sequence (CDS)

ATGGAAGAGGAAGAGGGAAGAAGGGCAGTGATGGAAAGAAAGGCAAAGCCAGGGAAAGATCAAATTTTGAAGTGCCCCAGATGCAATTCCAACAACACAAAGTTCTGTTATTACAACAATTACAGCCTCTCACAGCCAAGGTACTTCTGCAAGTCTTGTAGAAGATATTGGACAGCAGGTGGATCTTTGAGGAACATTCCAGTGGGTGGGGCCTCAAGGAAGAACAAAAGACCTTCAGCTAATTTTTCATCATCAGCAGCAGCAGCTGCAGCTCCTTCCAGTTCATCACCAAACAACATGAACAATAACAAGAACAGTGATAATAATAATGGTGATGATCACAGCAACAGCAACCCCATTATCATCAACAATGGCAGCTCCCAACCAAATCTCAATACTTCTTCTTCTTCTTCTTCCAATACTGCTTGTTGTTTGCAATTAATATTGGATTTCCCATTTCCATATGTAATCAGCATATTCTCTCTCCCTAGGAAGAAAAAAAATAGAACACTTTTGTGGATATTTCTAAAATTGTGA

Protein sequence

MEEEEGRRAVMERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGSLRNIPVGGASRKNKRPSANFSSSAAAAAAPSSSSPNNMNNNKNSDNNNGDDHSNSNPIIINNGSSQPNLNTSSSSSSNTACCLQLILDFPFPYVISIFSLPRKKKNRTLLWIFLKL

Homology

[Close]

BLAST of Lag0020148 vs. NCBI nr
Match: XP_004146271.1 (dof zinc finger protein DOF1.8 [Cucumis sativus] >XP_011651310.1 dof zinc finger protein DOF1.8 [Cucumis sativus] >XP_011651312.1 dof zinc finger protein DOF1.8 [Cucumis sativus] >XP_031737984.1 dof zinc finger protein DOF1.8 [Cucumis sativus] >XP_031737985.1 dof zinc finger protein DOF1.8 [Cucumis sativus] >KGN57649.1 hypothetical protein Csa_011544 [Cucumis sativus])

HSP 1 Score: 161.0 bits (406), Expect = 9.5e-36
Identity = 95/148 (64.19%), Postives = 105/148 (70.95%), Query Frame = 0


Query: 3   EEEGRRAVMERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGS 62
           EEEG +AV+ERKAK  KDQIL CPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGS
Sbjct: 17  EEEGLKAVVERKAKARKDQILNCPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGS 76

Query: 63  LRNIPVGGASRKNKRPSANFSSSAAAAAAPSSSSPNNMNNNKNSDNNNGDDHSNSNPIII 122
           LRNIPVGGASRKNKRPSANF            S P + NN KN +N+ GDD         
Sbjct: 77  LRNIPVGGASRKNKRPSANF------------SLPPSKNNQKNYNNDGGDDD-------- 136

Query: 123 NNGSSQPNLNTSSSSS------SNTACC 145
           + G SQ N+N +S SS      + T+CC
Sbjct: 137 DQGISQLNINITSCSSTATTTNTATSCC 144

[Close]

BLAST of Lag0020148 vs. NCBI nr
Match: XP_038895823.1 (dof zinc finger protein DOF4.6-like [Benincasa hispida])

HSP 1 Score: 159.8 bits (403), Expect = 2.1e-35
Identity = 90/142 (63.38%), Postives = 106/142 (74.65%), Query Frame = 0


Query: 3   EEEGRRAVMERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGS 62
           EEEG +AV+ERK K  KDQIL CPRCNSNNTKFCYYNNYSL+QPRYFCKSCRRYWTAGGS
Sbjct: 17  EEEGIKAVVERKEKARKDQILNCPRCNSNNTKFCYYNNYSLAQPRYFCKSCRRYWTAGGS 76

Query: 63  LRNIPVGGASRKNKRPSANFSSSAAAAAAPSSSSPNNMNNNKNSDNNNGDDHSNSNPIII 122
           LRNIPVGGASRKNKRPSANF            SSP + NN KN +N + DDH + + + I
Sbjct: 77  LRNIPVGGASRKNKRPSANF------------SSPPSKNNQKNYNNGDDDDHQDISQLNI 136

Query: 123 NNGSSQPNLNTSSSSSSNTACC 145
           N  S      +S+++++ T+CC
Sbjct: 137 NTTSC-----SSTAANTATSCC 141

[Close]

BLAST of Lag0020148 vs. NCBI nr
Match: XP_008455989.1 (PREDICTED: dof zinc finger protein DOF1.8-like [Cucumis melo] >KAA0050535.1 dof zinc finger protein DOF1.8-like [Cucumis melo var. makuwa] >TYK11276.1 dof zinc finger protein DOF1.8-like [Cucumis melo var. makuwa])

HSP 1 Score: 159.8 bits (403), Expect = 2.1e-35
Identity = 97/150 (64.67%), Postives = 106/150 (70.67%), Query Frame = 0


Query: 1   MEEEEGRRAVMERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAG 60
           +EEEEG +AV+ERKAK  KDQIL CPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAG
Sbjct: 16  LEEEEGIKAVVERKAKARKDQILNCPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAG 75

Query: 61  GSLRNIPVGGASRKNKRPSANFSSSAAAAAAPSSSSPNNMNNNKNSDNNNGDDHSNSNPI 120
           GSLRNIPVGGASRKNKRPSANF            S P + NN KN +N  G D       
Sbjct: 76  GSLRNIPVGGASRKNKRPSANF------------SLPPSKNNQKNYNNGGGGDD------ 135

Query: 121 IINNGSSQPNLNTSSSSS----SNTA--CC 145
             + G SQ N+N +S SS    +NTA  CC
Sbjct: 136 --DQGISQLNINITSCSSTATTTNTAATCC 145

[Close]

BLAST of Lag0020148 vs. NCBI nr
Match: XP_022984510.1 (dof zinc finger protein DOF5.7-like [Cucurbita maxima])

HSP 1 Score: 158.3 bits (399), Expect = 6.1e-35
Identity = 91/142 (64.08%), Postives = 98/142 (69.01%), Query Frame = 0


Query: 3   EEEGRRAVMERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGS 62
           EEEGRRA +ERKAKPGKDQIL CPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGS
Sbjct: 17  EEEGRRAGLERKAKPGKDQILNCPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGS 76

Query: 63  LRNIPVGGASRKNKRPSANFSSSAAAAAAPSSSSPNNMNNNKNSDNNNGDDHSNSNPIII 122
           LRNIPVGGASRKNKRP             PS + P+   NN     +NGDD    N    
Sbjct: 77  LRNIPVGGASRKNKRP-------------PSPNFPSPPKNNNKDYMSNGDDDDQGN---- 136

Query: 123 NNGSSQPNLNTSSSSSSNTACC 145
              S   N+NT   S++N A C
Sbjct: 137 ---SQSSNINTPCPSATNIATC 138

[Close]

BLAST of Lag0020148 vs. NCBI nr
Match: XP_023553431.1 (dof zinc finger protein DOF5.7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 157.1 bits (396), Expect = 1.4e-34
Identity = 90/142 (63.38%), Postives = 98/142 (69.01%), Query Frame = 0


Query: 3   EEEGRRAVMERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGS 62
           EEEGR+A +ERKAKPGKDQIL CPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGS
Sbjct: 17  EEEGRKAGLERKAKPGKDQILNCPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGS 76

Query: 63  LRNIPVGGASRKNKRPSANFSSSAAAAAAPSSSSPNNMNNNKNSDNNNGDDHSNSNPIII 122
           LRNIPVGGASRKNKRP             PS + P+   NN     +NGDD    N    
Sbjct: 77  LRNIPVGGASRKNKRP-------------PSPNFPSPPKNNNKDYMSNGDDDDQGN---- 136

Query: 123 NNGSSQPNLNTSSSSSSNTACC 145
              S   N+NT   S++N A C
Sbjct: 137 ---SQSSNINTPCPSATNIATC 138

[Close]

BLAST of Lag0020148 vs. ExPASy Swiss-Prot
Match: Q84JQ8 (Dof zinc finger protein DOF1.8 OS=Arabidopsis thaliana OX=3702 GN=DOF1.8 PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 6.2e-30
Identity = 68/93 (73.12%), Postives = 77/93 (82.80%), Query Frame = 0


Query: 11  MERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGSLRNIPVGG 70
           +ERKA+P KDQ L CPRCNS NTKFCYYNNYSL+QPRYFCK CRRYWTAGGSLRNIPVGG
Sbjct: 37  VERKARPEKDQALNCPRCNSLNTKFCYYNNYSLTQPRYFCKDCRRYWTAGGSLRNIPVGG 96

Query: 71  ASRKNKRPSANFSSSAAAAAAPSSSSPNNMNNN 104
             RKNKR S+N SSS++ +++ SS  P   NNN
Sbjct: 97  GVRKNKRSSSN-SSSSSPSSSSSSKKPLFANNN 128

[Close]

BLAST of Lag0020148 vs. ExPASy Swiss-Prot
Match: B9F1L8 (Dof zinc finger protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=DOF2 PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 2.3e-29
Identity = 62/90 (68.89%), Postives = 74/90 (82.22%), Query Frame = 0


Query: 6   GRRAVMERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGSLRN 65
           G  A  ER+A+P K++ L CPRCNS NTKFCYYNNYSL QPRYFCK+CRRYWT GGSLRN
Sbjct: 63  GAGAGTERRARPQKEKALNCPRCNSTNTKFCYYNNYSLQQPRYFCKTCRRYWTEGGSLRN 122

Query: 66  IPVGGASRKNKRPSANFSSSAAAAAAPSSS 96
           +PVGG SRKNKR S++   SAAA+A+ S++
Sbjct: 123 VPVGGGSRKNKRSSSSVVPSAAASASTSAA 152

[Close]

BLAST of Lag0020148 vs. ExPASy Swiss-Prot
Match: Q8LAP8 (Dof zinc finger protein DOF4.6 OS=Arabidopsis thaliana OX=3702 GN=DOF4.6 PE=2 SV=2)

HSP 1 Score: 123.6 bits (309), Expect = 2.2e-27
Identity = 59/86 (68.60%), Postives = 70/86 (81.40%), Query Frame = 0


Query: 12  ERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGSLRNIPVGGA 71
           ERKA+P KDQ + CPRCNS NTKFCYYNNYSL+QPRYFCK CRRYWT GGSLRNIPVGG 
Sbjct: 42  ERKARPEKDQAVNCPRCNSTNTKFCYYNNYSLTQPRYFCKGCRRYWTEGGSLRNIPVGGG 101

Query: 72  SRKNKR---PSANFSSSAAAAAAPSS 95
           SRKNKR    S++ S++ + +  P++
Sbjct: 102 SRKNKRSHSSSSDISNNHSDSTQPAT 127

[Close]

BLAST of Lag0020148 vs. ExPASy Swiss-Prot
Match: Q9SXG8 (Dof zinc finger protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DOF1 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 3.2e-26
Identity = 64/120 (53.33%), Postives = 90/120 (75.00%), Query Frame = 0


Query: 12  ERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGSLRNIPVGGA 71
           ER+A+P K++ L CPRCNS NTKFCYYNNYSL QPRYFCK+CRRYWT GGSLRN+PVGG 
Sbjct: 67  ERRARPQKEKALNCPRCNSTNTKFCYYNNYSLQQPRYFCKTCRRYWTEGGSLRNVPVGGG 126

Query: 72  SRKNKRPSANFSSSAAAAAAPSSSSPNNMNNNKNSDNNNGDDHSNSNPIIINNGSSQPNL 131
           SRKNKR S++ +S++ A+A+ ++S   + + + +  +  G   ++ NP +++ G+   NL
Sbjct: 127 SRKNKRSSSSAASASPASASTANSVVTSASMSMSMASTGGG--ASKNPKLVHEGAQDLNL 184

[Close]

BLAST of Lag0020148 vs. ExPASy Swiss-Prot
Match: Q9ZPY0 (Dof zinc finger protein DOF2.5 OS=Arabidopsis thaliana OX=3702 GN=DOF2.5 PE=2 SV=3)

HSP 1 Score: 119.8 bits (299), Expect = 3.2e-26
Identity = 61/85 (71.76%), Postives = 68/85 (80.00%), Query Frame = 0


Query: 10  VMERKAKPGKDQILKCPRCNSNNTKFCYYNNYSLSQPRYFCKSCRRYWTAGGSLRNIPVG 69
           V ERKA+P   + L CPRCNS NTKFCYYNNYSL+QPRYFCK CRRYWT GGSLRN+PVG
Sbjct: 69  VAERKARP--QEKLNCPRCNSTNTKFCYYNNYSLTQPRYFCKGCRRYWTEGGSLRNVPVG 128

Query: 70  GASRKNKRPSANFSSSAAAAAAPSS 95
           G+SRKNKR S++ SSS      PSS
Sbjct: 129 GSSRKNKRSSSS-SSSNILQTIPSS 150

The following BLAST results are available for this feature:

BLAST of Lag0020148 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146271.1	9.5e-36	64.19	dof zinc finger protein DOF1.8 [Cucumis sativus] >XP_011651310.1 dof zinc finger...	[more]
XP_038895823.1	2.1e-35	63.38	dof zinc finger protein DOF4.6-like [Benincasa hispida]	[more]
XP_008455989.1	2.1e-35	64.67	PREDICTED: dof zinc finger protein DOF1.8-like [Cucumis melo] >KAA0050535.1 dof ...	[more]
XP_022984510.1	6.1e-35	64.08	dof zinc finger protein DOF5.7-like [Cucurbita maxima]	[more]
XP_023553431.1	1.4e-34	63.38	dof zinc finger protein DOF5.7-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0020148 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84JQ8	6.2e-30	73.12	Dof zinc finger protein DOF1.8 OS=Arabidopsis thaliana OX=3702 GN=DOF1.8 PE=2 SV...	[more]
B9F1L8	2.3e-29	68.89	Dof zinc finger protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=DOF2 PE=2 ...	[more]
Q8LAP8	2.2e-27	68.60	Dof zinc finger protein DOF4.6 OS=Arabidopsis thaliana OX=3702 GN=DOF4.6 PE=2 SV...	[more]
Q9SXG8	3.2e-26	53.33	Dof zinc finger protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DOF1 PE=2 ...	[more]
Q9ZPY0	3.2e-26	71.76	Dof zinc finger protein DOF2.5 OS=Arabidopsis thaliana OX=3702 GN=DOF2.5 PE=2 SV...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003851	Zinc finger, Dof-type	PFAM	PF02701	zf-Dof	coord: 20..77 e-value: 8.7E-34 score: 115.5
IPR003851	Zinc finger, Dof-type	PROSITE	PS01361	ZF_DOF_1	coord: 25..61
IPR003851	Zinc finger, Dof-type	PROSITE	PS50884	ZF_DOF_2	coord: 23..77 score: 29.086916
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 67..135
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 76..135
None	No IPR available	PANTHER	PTHR31992:SF124	DOF ZINC FINGER PROTEIN DOF1.8	coord: 9..147
None	No IPR available	PANTHER	PTHR31992	DOF ZINC FINGER PROTEIN DOF1.4-RELATED	coord: 9..147

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0020148.1	Lag0020148.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding