Homology
BLAST of Lag0019798 vs. NCBI nr
Match:
KAG6600225.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1A, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 979.2 bits (2530), Expect = 1.8e-281
Identity = 527/588 (89.63%), Postives = 543/588 (92.35%), Query Frame = 0
Query: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLV+MCILWQL
Sbjct: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
Query: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFL MVTALLLIKALHWLA
Sbjct: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180
QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLF YNSI SLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
ILATTAVSTFVKYIFYVSDVLMEGQWE+KPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANQQ--GTT 360
VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANQQ GTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANQQGTGTT 360
Query: 361 NSSTQGSSGTGVAGDNLTHHQARVRAAAAAASIYEKSYVYPFASTLVWSPGYAVVPQVQR 420
+SS QGS GT AGD+L+HHQARV+AAAAAASIYEKSYVYPF +TL WSPGYAVVPQVQR
Sbjct: 361 SSSAQGSGGTSAAGDSLSHHQARVQAAAAAASIYEKSYVYPFGNTLAWSPGYAVVPQVQR 420
Query: 421 PLPDSNNIESSGERAPNEQPQYFPFTGGPANLSFTQIPQNLFVPFQPREGTVNHTDGLGS 480
PL DSNN+E GE+APNEQ QYFPFTGGPANLSFTQIPQNLFVPFQPREGTVN+TDGLGS
Sbjct: 421 PLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFVPFQPREGTVNNTDGLGS 480
Query: 481 SQNISGSQLEAQERFLEQQIEILKDQLQLLRKPKVEESSRAAGEADKKGKSVASSSPSSS 540
SQNISGSQLE+QERFL+QQIEIL+DQLQLLRK KVEESS AGEADKKGKS+ASSS SSS
Sbjct: 481 SQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESS-IAGEADKKGKSIASSSQSSS 540
Query: 541 SSVSDSQAPGEIER-----MPALHQSKATTSRLSFQFIPIFSANFPFS 582
SSVSDS PGE ER +P S AT F F F N F+
Sbjct: 541 SSVSDSHPPGETERTFLLLIPWKPASSATGQFFRFFFFFFFVHNIFFN 587
BLAST of Lag0019798 vs. NCBI nr
Match:
KAG7030884.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1A [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 974.9 bits (2519), Expect = 3.5e-280
Identity = 517/554 (93.32%), Postives = 531/554 (95.85%), Query Frame = 0
Query: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLV+MCILWQL
Sbjct: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
Query: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFL MVTALLLIKALHWLA
Sbjct: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180
QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLF YNSI SLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
ILATTAVSTFVKYIFYVSDVLMEGQWE+KPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANQQ--GTT 360
VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANQQ GTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANQQGTGTT 360
Query: 361 NSSTQGSSGTGVAGDNLTHHQARVRAAAAAASIYEKSYVYPFASTLVWSPGYAVVPQVQR 420
+SS QGS GT AGD+L+HHQARV+AAAAAASIYEKSYVYPF +TL WSPGYAVVPQVQR
Sbjct: 361 SSSAQGSGGTSAAGDSLSHHQARVQAAAAAASIYEKSYVYPFGNTLAWSPGYAVVPQVQR 420
Query: 421 PLPDSNNIESSGERAPNEQPQYFPFTGGPANLSFTQIPQNLFVPFQPREGTVNHTDGLGS 480
PL DSNN+E GE+APNEQ QYFPFTGGPANLS TQIPQNLFVPFQPREGTVN+TDGLGS
Sbjct: 421 PLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSCTQIPQNLFVPFQPREGTVNNTDGLGS 480
Query: 481 SQNISGSQLEAQERFLEQQIEILKDQLQLLRKPKVEESSRAAGEADKKGKSVASSSPSSS 540
SQNISGSQLE+QERFL+QQIEIL+DQLQLLRK KVEESS AGEADKKGKS+ASSS SSS
Sbjct: 481 SQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESS-IAGEADKKGKSIASSSQSSS 540
Query: 541 SSVSDSQAPGEIER 553
SSVSDS PGE ER
Sbjct: 541 SSVSDSHPPGETER 553
BLAST of Lag0019798 vs. NCBI nr
Match:
XP_023531768.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita pepo subsp. pepo] >XP_023531774.1 ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 970.7 bits (2508), Expect = 6.5e-279
Identity = 515/554 (92.96%), Postives = 529/554 (95.49%), Query Frame = 0
Query: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLV+MCILWQL
Sbjct: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
Query: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFL MVTALLLIKALHWLA
Sbjct: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180
QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLF YNSI SLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
ILATTAVSTFVKYIFYVSDVLMEGQWE+KPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANQQ--GTT 360
VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQG QSDANQQ GTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGSQSDANQQGTGTT 360
Query: 361 NSSTQGSSGTGVAGDNLTHHQARVRAAAAAASIYEKSYVYPFASTLVWSPGYAVVPQVQR 420
+SS QGS GTG GD+L+HHQARV+AAAAAASIYEKSYVYPFA+TL WSPGYAVVPQVQR
Sbjct: 361 SSSAQGSGGTGAVGDSLSHHQARVQAAAAAASIYEKSYVYPFANTLAWSPGYAVVPQVQR 420
Query: 421 PLPDSNNIESSGERAPNEQPQYFPFTGGPANLSFTQIPQNLFVPFQPREGTVNHTDGLGS 480
PL DSNN+E GE+A NEQ QYFPFT GPANLSFTQIPQNLFVPFQPREGTVN+TDGLGS
Sbjct: 421 PLLDSNNVEPIGEQASNEQAQYFPFTSGPANLSFTQIPQNLFVPFQPREGTVNNTDGLGS 480
Query: 481 SQNISGSQLEAQERFLEQQIEILKDQLQLLRKPKVEESSRAAGEADKKGKSVASSSPSSS 540
SQN SGSQLEAQERFL+QQIEIL+DQLQLLRK KVE+SS AGEADKKGKS+ASSS SSS
Sbjct: 481 SQNTSGSQLEAQERFLQQQIEILQDQLQLLRKLKVEDSS-IAGEADKKGKSIASSSQSSS 540
Query: 541 SSVSDSQAPGEIER 553
SSVSDS PGE ER
Sbjct: 541 SSVSDSHPPGETER 553
BLAST of Lag0019798 vs. NCBI nr
Match:
XP_022942315.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita moschata] >XP_022942316.1 ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita moschata])
HSP 1 Score: 969.9 bits (2506), Expect = 1.1e-278
Identity = 514/554 (92.78%), Postives = 529/554 (95.49%), Query Frame = 0
Query: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLV+MCILWQL
Sbjct: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
Query: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFL MVTALLLIKALHWLA
Sbjct: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180
QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLF YNSI SLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
ILATTAVSTFVKYIFYVSDVLMEGQWE+KPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANQQ--GTT 360
VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDAN Q GTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANLQGTGTT 360
Query: 361 NSSTQGSSGTGVAGDNLTHHQARVRAAAAAASIYEKSYVYPFASTLVWSPGYAVVPQVQR 420
+SS QGS GT AGD+L HHQARV+AAAAAASIYEKSYVYPF +TL WSPGYAV+PQVQR
Sbjct: 361 SSSAQGSGGTSAAGDSLGHHQARVQAAAAAASIYEKSYVYPFGNTLAWSPGYAVIPQVQR 420
Query: 421 PLPDSNNIESSGERAPNEQPQYFPFTGGPANLSFTQIPQNLFVPFQPREGTVNHTDGLGS 480
PL DSNN+E GE+APNEQ QYFPFTGGPANLSFTQIPQNLF+PFQPREGTVN+TDGLGS
Sbjct: 421 PLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFIPFQPREGTVNNTDGLGS 480
Query: 481 SQNISGSQLEAQERFLEQQIEILKDQLQLLRKPKVEESSRAAGEADKKGKSVASSSPSSS 540
SQNISGSQLE+QERFL+QQIEIL+DQLQLLRK KVEESS AGEADKKGKS+ASSS SSS
Sbjct: 481 SQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESS-IAGEADKKGKSIASSSQSSS 540
Query: 541 SSVSDSQAPGEIER 553
SSVSDS GE ER
Sbjct: 541 SSVSDSHPHGETER 553
BLAST of Lag0019798 vs. NCBI nr
Match:
XP_022141279.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like isoform X2 [Momordica charantia])
HSP 1 Score: 968.0 bits (2501), Expect = 4.2e-278
Identity = 513/554 (92.60%), Postives = 530/554 (95.67%), Query Frame = 0
Query: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
Query: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA
Sbjct: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180
QKRVEYMETTPSVPLLSHIRIVSFL FLF+LDSLFFYNSIE LI+TRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLSFLFILDSLFFYNSIEYLIRTRKASVSLFFSFEYM 180
Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
ILATTAVSTFVKYIFYVSDVLMEGQWE+KPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANQQGTTNS 360
VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGH G DANQQGTT+S
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHHG-SHDANQQGTTSS 360
Query: 361 STQGSSGTGVAGDNLTHHQARVRAAAAAASIYEKSYVYPFASTLVWSPGYAVVPQVQRPL 420
S Q SSGTGVAGDNL+HHQARV+AAAAAASIYEKSYVYP ASTLVWSPGYAVVPQ QRPL
Sbjct: 361 SAQDSSGTGVAGDNLSHHQARVQAAAAAASIYEKSYVYPCASTLVWSPGYAVVPQTQRPL 420
Query: 421 PDSNNIESSGERAPNEQPQYFPFTGGPANLSFTQIPQNLFVPFQPREGTVNHTDGLGSSQ 480
DSNNIE +GE+APN QPQYFPFTGGPANLSFTQ PQNLFVPFQPREG+VN+TDGLGSSQ
Sbjct: 421 ADSNNIEPNGEQAPNGQPQYFPFTGGPANLSFTQFPQNLFVPFQPREGSVNNTDGLGSSQ 480
Query: 481 NISGSQLEAQERFLEQQIEILKDQLQLLRKPKVEESSRAAGEADKKGKSVASSSPSSSSS 540
N+SG LE QERF++QQIE+L+ QLQLLR PKVEES+R AGEADKKGKSVASS PSSSSS
Sbjct: 481 NVSGLHLEDQERFIQQQIEMLQKQLQLLRNPKVEESTR-AGEADKKGKSVASSPPSSSSS 540
Query: 541 VSDSQAP-GEIERM 554
VS+S P GEIERM
Sbjct: 541 VSESHPPAGEIERM 552
BLAST of Lag0019798 vs. ExPASy Swiss-Prot
Match:
Q9LW77 (ERAD-associated E3 ubiquitin-protein ligase HRD1A OS=Arabidopsis thaliana OX=3702 GN=HRD1A PE=2 SV=1)
HSP 1 Score: 609.4 bits (1570), Expect = 5.0e-173
Identity = 339/537 (63.13%), Postives = 403/537 (75.05%), Query Frame = 0
Query: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
M++L+TYAG+SF+AT+ VIYHAF+SRGQFYPA VYLSTSKI+LVLLLNM LV+M LW L
Sbjct: 1 MIRLRTYAGLSFMATLAVIYHAFSSRGQFYPATVYLSTSKISLVLLLNMCLVLMLSLWHL 60
Query: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
K +FLGSLREAEVERLNEQ+W+ELMEILFAITIFRQDFS FL +V LLLIKALHWLA
Sbjct: 61 VKFVFLGSLREAEVERLNEQAWRELMEILFAITIFRQDFSSGFLPLVVTLLLIKALHWLA 120
Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180
QKRVEY+ETTPSV LSH RIVSF+GFL ++DSLF Y+SI LIQ+R+ASVSLFFSFEYM
Sbjct: 121 QKRVEYIETTPSVSKLSHFRIVSFMGFLLLVDSLFMYSSIRHLIQSRQASVSLFFSFEYM 180
Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
ILATT V+ FVKY+FYV+D+LM+GQWE+KPVYTFYLELIRDLLHLSMY+CFF VIFM YG
Sbjct: 181 ILATTTVAIFVKYVFYVTDMLMDGQWEKKPVYTFYLELIRDLLHLSMYICFFFVIFMNYG 240
Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
VPLHL+RELYE FRNF+IR++DY+RYRKITSNMNDRFPDATPEEL +SDATCIICREEMT
Sbjct: 241 VPLHLLRELYETFRNFQIRVSDYLRYRKITSNMNDRFPDATPEELTASDATCIICREEMT 300
Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANQQGTTNS 360
AKKL+CGHLFHVHCLRSWLERQ TCPTCRALVVPPE +++A Q +QQGT++S
Sbjct: 301 NAKKLICGHLFHVHCLRSWLERQQTCPTCRALVVPPENATSAAPGQRELHQGSQQGTSSS 360
Query: 361 STQG---SSGTGVAGDNLTHHQARVRAAAAAASIYEKSYVYPFASTLVWSPGYAVVPQVQ 420
QG SS GV+ ++L+ H AR++AAA+AAS+Y KS VYP A+T+ WS G VP
Sbjct: 361 GNQGSEISSSAGVSNNSLSRHHARLQAAASAASVYGKSMVYPSANTVAWSSG---VP--- 420
Query: 421 RPLPDSNNIESSGERAPNEQPQYFPFTGGPANLSFTQIPQNLFVPFQPREGTVNHTDGLG 480
E+ E Q P P S
Sbjct: 421 -----------GTEQVSTEPDQTLPQHNLPVENSH------------------------- 480
Query: 481 SSQNISGSQLEAQERFLEQQIEILKDQLQLLRKPKVEESSRAAGEADKKGKSVASSS 535
+ N+S ++LE + LE +EIL+++L L K E +AGE + KGKSVA ++
Sbjct: 481 AYANMSETKLEEMRKSLETHLEILRNRLHFLETRKPE----SAGEPENKGKSVADAA 491
BLAST of Lag0019798 vs. ExPASy Swiss-Prot
Match:
Q6NPT7 (ERAD-associated E3 ubiquitin-protein ligase HRD1B OS=Arabidopsis thaliana OX=3702 GN=HRD1B PE=2 SV=1)
HSP 1 Score: 601.3 bits (1549), Expect = 1.4e-170
Identity = 335/538 (62.27%), Postives = 397/538 (73.79%), Query Frame = 0
Query: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
M++L+ YAG+S +AT+VVIYHAF+SRGQFYPA VYLSTSKINLV+LLNMGLV+M LW L
Sbjct: 1 MIQLKVYAGLSTLATLVVIYHAFSSRGQFYPATVYLSTSKINLVVLLNMGLVLMLSLWNL 60
Query: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
K +FLGSLREAEVERLNEQ+W+ELMEILFAITIFRQDFSV F+ +V LLLIK LHW+A
Sbjct: 61 VKIVFLGSLREAEVERLNEQAWRELMEILFAITIFRQDFSVGFISLVVTLLLIKGLHWMA 120
Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180
QKRVEY+ETTPSV LLSH+RIVSF+ FL +LD L Y+SI+ LIQ+RKAS+S+FF+FEYM
Sbjct: 121 QKRVEYIETTPSVTLLSHVRIVSFMVFLLILDCLLTYSSIQQLIQSRKASMSVFFTFEYM 180
Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
ILATT VS VKY FYV+D+L EGQWE KPVYTFYLEL+RDLLHLSMYLCFFL+IFM YG
Sbjct: 181 ILATTTVSIIVKYAFYVTDMLKEGQWEGKPVYTFYLELVRDLLHLSMYLCFFLMIFMNYG 240
Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
+PLHLIRELYE FRNF+IR+ DY+RYRKITSNMNDRFPDATPEEL+S+DATCIICREEMT
Sbjct: 241 LPLHLIRELYETFRNFKIRVTDYLRYRKITSNMNDRFPDATPEELSSNDATCIICREEMT 300
Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPE-GSSTAGGHQGLQSDANQQGTTN 360
AKKLVCGHLFHVHCLRSWLERQ+TCPTCRALVVP E +STA G++G ++ QQGT
Sbjct: 301 SAKKLVCGHLFHVHCLRSWLERQNTCPTCRALVVPAENATSTASGNRGPHQESLQQGTGT 360
Query: 361 SST--QGSSGTGVAGDNLTHHQARVRAAAAAASIYEKSYVYP-FASTLVWSPGYAVVPQV 420
SS+ QGSS + A +N++ H+AR +AAA+AASIY +S VYP A+TLVWS G +++PQ
Sbjct: 361 SSSDGQGSSVSAAASENMSRHEARFQAAASAASIYGRSIVYPSSANTLVWSQGNSLLPQ- 420
Query: 421 QRPLPDSNNIESSGERAPNEQPQYFPFTGGPANLSFTQIPQNLFVPFQPREGTVNHTDGL 480
Sbjct: 421 ------------------------------------------------------------ 460
Query: 481 GSSQNISGSQLEAQERFLEQQIEILKDQLQLLRKPKVEESSRAAGEADKKGKSVASSS 535
+++EAQ RFLE QIE+L +QL+LL KP D KGKSVA ++
Sbjct: 481 --------TEVEAQRRFLESQIEVLTNQLRLLEKPTT---------VDTKGKSVADTA 460
BLAST of Lag0019798 vs. ExPASy Swiss-Prot
Match:
Q5Z880 (ERAD-associated E3 ubiquitin-protein ligase HRD1 OS=Oryza sativa subsp. japonica OX=39947 GN=HRD1 PE=2 SV=1)
HSP 1 Score: 565.1 bits (1455), Expect = 1.1e-159
Identity = 308/509 (60.51%), Postives = 382/509 (75.05%), Query Frame = 0
Query: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
M++LQTYA S +AT +Y+AF+SR QFYPA+VYLSTSKI VLLLN GLV MC+ WQL
Sbjct: 1 MIRLQTYAAFSLMATATAVYYAFSSREQFYPAMVYLSTSKICFVLLLNTGLVAMCVAWQL 60
Query: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
K++FLG+LREAEVERLNEQ+W+E++EILFA+TIFRQDFSV+FL MV ALLL+KALHWLA
Sbjct: 61 VKRLFLGTLREAEVERLNEQAWREVVEILFAVTIFRQDFSVSFLAMVAALLLVKALHWLA 120
Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180
QKRVEY+ETTPSVP+LSH RIVSF+ FL V+D LF NS+ SLI R+ASV++FFSFEYM
Sbjct: 121 QKRVEYIETTPSVPMLSHARIVSFMLFLLVVDCLFLSNSLRSLIHKREASVAIFFSFEYM 180
Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
ILAT+ VSTFVKYIFYVSD+LMEGQWE+K VYTFYLELI DL+HLS+Y+ FF+ IF+ YG
Sbjct: 181 ILATSTVSTFVKYIFYVSDMLMEGQWEKKAVYTFYLELISDLVHLSLYMLFFIAIFLNYG 240
Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
VPLHLIRELYE FRNFRIRIADY+RYRKITSNMN+RFPDAT +ELN+SDATCIICREEMT
Sbjct: 241 VPLHLIRELYETFRNFRIRIADYVRYRKITSNMNERFPDATADELNASDATCIICREEMT 300
Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANQQGTTNS 360
AKKL+CGHLFHVHCLRSWLERQHTCPTCRA ++PP+ TA G+ Q +
Sbjct: 301 TAKKLLCGHLFHVHCLRSWLERQHTCPTCRAPILPPDNGRTAARPHGVHPGV--QPVPGN 360
Query: 361 STQGSSGTGVAGDNLTHHQARVRAAAAAASIYEKSYVYPFASTLVWSPGYAVVPQVQRPL 420
T GS AG+N++ QA++ AAA+AAS+Y +S+ YP A+ L Y+ PQ
Sbjct: 361 GTPGSE--RAAGENISRRQAKLEAAASAASLYGRSFAYPPANNL---NRYSTPPQ----- 420
Query: 421 PDSNNIESSGERAPNEQPQYFPFTGGPANLSFTQIPQNLFVPFQPREGTVNHTDGLGSSQ 480
++N SGE + + Q T P+ +F G V+ + +++
Sbjct: 421 -STSNGPQSGEASTSNQSPKGHATADPSAPTFY------------ARGAVS---SVTTTR 480
Query: 481 NISGSQLEAQERFLEQQIEILKDQLQLLR 510
+ S +A E ++ QIE+L+ QLQ+ +
Sbjct: 481 ELESSLQKAYENAIKSQIEMLQIQLQMFQ 481
BLAST of Lag0019798 vs. ExPASy Swiss-Prot
Match:
Q803I8 (E3 ubiquitin-protein ligase synoviolin OS=Danio rerio OX=7955 GN=syvn1 PE=2 SV=2)
HSP 1 Score: 263.8 bits (673), Expect = 5.2e-69
Identity = 141/331 (42.60%), Postives = 201/331 (60.73%), Query Frame = 0
Query: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
M++ S T V+ HA+ + QFYP +VYL+ S ++ +L V++ +L +L
Sbjct: 1 MVRAALVTATSLALTGAVVAHAYFLKHQFYPTVVYLTKSSPSMAVLYIQAFVLVFLLGKL 60
Query: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
+K+F G LR AE+E L E+SW + E A T+FR DFS F+ + T LL +K HWLA
Sbjct: 61 MRKVFFGQLRAAEMEHLIERSWYAVTETCLAFTVFRDDFSPRFVALFTLLLFLKCFHWLA 120
Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180
+ RV++ME +P++ + H R++S + L V+D LF ++ S+I TR ASV L F FEY
Sbjct: 121 EDRVDFMERSPNISWVFHFRVLSLMVLLGVMDFLFVNHACHSII-TRGASVQLVFGFEYA 180
Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
IL T ++TF+KY + D+ E W+ K VY Y EL + + +Y+ F ++ +
Sbjct: 181 ILMTMVLTTFIKYTLHTIDLQSENPWDNKAVYMLYTELFTGFIKVLLYMAFMTIMIKVHT 240
Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEM- 300
PL IR +Y R F+ + D I R+ NMN +PDATPE+L ++D CIICREEM
Sbjct: 241 FPLFAIRPMYLAMRQFKKAVTDAIMSRRAIRNMNTLYPDATPEDLQATDNVCIICREEMV 300
Query: 301 TVAKKLVCGHLFHVHCLRSWLERQHTCPTCR 331
T AKKL C H+FH CLRSW +RQ TCPTCR
Sbjct: 301 TGAKKLPCNHIFHSSCLRSWFQRQQTCPTCR 330
BLAST of Lag0019798 vs. ExPASy Swiss-Prot
Match:
Q86TM6 (E3 ubiquitin-protein ligase synoviolin OS=Homo sapiens OX=9606 GN=SYVN1 PE=1 SV=2)
HSP 1 Score: 261.5 bits (667), Expect = 2.6e-68
Identity = 141/331 (42.60%), Postives = 198/331 (59.82%), Query Frame = 0
Query: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
M + S T V+ HA+ + QFYP +VYL+ S ++ +L V++ +L ++
Sbjct: 1 MFRTAVMMAASLALTGAVVAHAYYLKHQFYPTVVYLTKSSPSMAVLYIQAFVLVFLLGKV 60
Query: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
K+F G LR AE+E L E+SW + E A T+FR DFS F+ + T LL +K HWLA
Sbjct: 61 MGKVFFGQLRAAEMEHLLERSWYAVTETCLAFTVFRDDFSPRFVALFTLLLFLKCFHWLA 120
Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180
+ RV++ME +P++ L H RIVS + L +LD LF ++ S++ TR ASV L F FEY
Sbjct: 121 EDRVDFMERSPNISWLFHCRIVSLMFLLGILDFLFVSHAYHSIL-TRGASVQLVFGFEYA 180
Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
IL T ++ F+KY+ + D+ E W+ K VY Y EL + + +Y+ F ++ +
Sbjct: 181 ILMTMVLTIFIKYVLHSVDLQSENPWDNKAVYMLYTELFTGFIKVLLYMAFMTIMIKVHT 240
Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEM- 300
PL IR +Y R F+ + D I R+ NMN +PDATPEEL + D CIICREEM
Sbjct: 241 FPLFAIRPMYLAMRQFKKAVTDAIMSRRAIRNMNTLYPDATPEELQAMDNVCIICREEMV 300
Query: 301 TVAKKLVCGHLFHVHCLRSWLERQHTCPTCR 331
T AK+L C H+FH CLRSW +RQ TCPTCR
Sbjct: 301 TGAKRLPCNHIFHTSCLRSWFQRQQTCPTCR 330
BLAST of Lag0019798 vs. ExPASy TrEMBL
Match:
A0A6J1FNJ3 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucurbita moschata OX=3662 GN=LOC111447399 PE=4 SV=1)
HSP 1 Score: 969.9 bits (2506), Expect = 5.4e-279
Identity = 514/554 (92.78%), Postives = 529/554 (95.49%), Query Frame = 0
Query: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLV+MCILWQL
Sbjct: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
Query: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFL MVTALLLIKALHWLA
Sbjct: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180
QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLF YNSI SLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
ILATTAVSTFVKYIFYVSDVLMEGQWE+KPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANQQ--GTT 360
VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDAN Q GTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANLQGTGTT 360
Query: 361 NSSTQGSSGTGVAGDNLTHHQARVRAAAAAASIYEKSYVYPFASTLVWSPGYAVVPQVQR 420
+SS QGS GT AGD+L HHQARV+AAAAAASIYEKSYVYPF +TL WSPGYAV+PQVQR
Sbjct: 361 SSSAQGSGGTSAAGDSLGHHQARVQAAAAAASIYEKSYVYPFGNTLAWSPGYAVIPQVQR 420
Query: 421 PLPDSNNIESSGERAPNEQPQYFPFTGGPANLSFTQIPQNLFVPFQPREGTVNHTDGLGS 480
PL DSNN+E GE+APNEQ QYFPFTGGPANLSFTQIPQNLF+PFQPREGTVN+TDGLGS
Sbjct: 421 PLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFIPFQPREGTVNNTDGLGS 480
Query: 481 SQNISGSQLEAQERFLEQQIEILKDQLQLLRKPKVEESSRAAGEADKKGKSVASSSPSSS 540
SQNISGSQLE+QERFL+QQIEIL+DQLQLLRK KVEESS AGEADKKGKS+ASSS SSS
Sbjct: 481 SQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESS-IAGEADKKGKSIASSSQSSS 540
Query: 541 SSVSDSQAPGEIER 553
SSVSDS GE ER
Sbjct: 541 SSVSDSHPHGETER 553
BLAST of Lag0019798 vs. ExPASy TrEMBL
Match:
A0A6J1CI55 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011717 PE=4 SV=1)
HSP 1 Score: 968.0 bits (2501), Expect = 2.1e-278
Identity = 513/554 (92.60%), Postives = 530/554 (95.67%), Query Frame = 0
Query: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
Query: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA
Sbjct: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180
QKRVEYMETTPSVPLLSHIRIVSFL FLF+LDSLFFYNSIE LI+TRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLSFLFILDSLFFYNSIEYLIRTRKASVSLFFSFEYM 180
Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
ILATTAVSTFVKYIFYVSDVLMEGQWE+KPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANQQGTTNS 360
VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGH G DANQQGTT+S
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHHG-SHDANQQGTTSS 360
Query: 361 STQGSSGTGVAGDNLTHHQARVRAAAAAASIYEKSYVYPFASTLVWSPGYAVVPQVQRPL 420
S Q SSGTGVAGDNL+HHQARV+AAAAAASIYEKSYVYP ASTLVWSPGYAVVPQ QRPL
Sbjct: 361 SAQDSSGTGVAGDNLSHHQARVQAAAAAASIYEKSYVYPCASTLVWSPGYAVVPQTQRPL 420
Query: 421 PDSNNIESSGERAPNEQPQYFPFTGGPANLSFTQIPQNLFVPFQPREGTVNHTDGLGSSQ 480
DSNNIE +GE+APN QPQYFPFTGGPANLSFTQ PQNLFVPFQPREG+VN+TDGLGSSQ
Sbjct: 421 ADSNNIEPNGEQAPNGQPQYFPFTGGPANLSFTQFPQNLFVPFQPREGSVNNTDGLGSSQ 480
Query: 481 NISGSQLEAQERFLEQQIEILKDQLQLLRKPKVEESSRAAGEADKKGKSVASSSPSSSSS 540
N+SG LE QERF++QQIE+L+ QLQLLR PKVEES+R AGEADKKGKSVASS PSSSSS
Sbjct: 481 NVSGLHLEDQERFIQQQIEMLQKQLQLLRNPKVEESTR-AGEADKKGKSVASSPPSSSSS 540
Query: 541 VSDSQAP-GEIERM 554
VS+S P GEIERM
Sbjct: 541 VSESHPPAGEIERM 552
BLAST of Lag0019798 vs. ExPASy TrEMBL
Match:
A0A6J1KWZ5 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499418 PE=4 SV=1)
HSP 1 Score: 967.6 bits (2500), Expect = 2.7e-278
Identity = 513/554 (92.60%), Postives = 528/554 (95.31%), Query Frame = 0
Query: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLV+MCILWQL
Sbjct: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
Query: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFL MVTALLLIKALHWLA
Sbjct: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180
QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLF YNSI SLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
ILATTAVSTFVKYIFYVSDVLMEGQWE+KPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANQQ--GTT 360
VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANQQ GTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANQQGTGTT 360
Query: 361 NSSTQGSSGTGVAGDNLTHHQARVRAAAAAASIYEKSYVYPFASTLVWSPGYAVVPQVQR 420
+SS QGS GT AGD+L+HHQ+RV+AAAAAASIYEKSYVYPF +TL WSPGYAVVPQVQR
Sbjct: 361 SSSEQGSGGTSAAGDSLSHHQSRVQAAAAAASIYEKSYVYPFGNTLAWSPGYAVVPQVQR 420
Query: 421 PLPDSNNIESSGERAPNEQPQYFPFTGGPANLSFTQIPQNLFVPFQPREGTVNHTDGLGS 480
PL DSNN+E GE+A NEQ QYFPF GGPANLS+TQIPQNLFVPFQPREGTVN+TDGL S
Sbjct: 421 PLLDSNNVEPIGEQAANEQAQYFPFAGGPANLSYTQIPQNLFVPFQPREGTVNNTDGLSS 480
Query: 481 SQNISGSQLEAQERFLEQQIEILKDQLQLLRKPKVEESSRAAGEADKKGKSVASSSPSSS 540
QNISGSQLEAQERFL+QQIEIL+DQLQLLRK KVEESS AGEADKKGKS+ASSS SSS
Sbjct: 481 GQNISGSQLEAQERFLQQQIEILQDQLQLLRKLKVEESS-IAGEADKKGKSIASSSQSSS 540
Query: 541 SSVSDSQAPGEIER 553
SSVSDS PGE ER
Sbjct: 541 SSVSDSHPPGETER 553
BLAST of Lag0019798 vs. ExPASy TrEMBL
Match:
A0A6J1CJF5 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011717 PE=4 SV=1)
HSP 1 Score: 963.0 bits (2488), Expect = 6.6e-277
Identity = 513/556 (92.27%), Postives = 530/556 (95.32%), Query Frame = 0
Query: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
Query: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA
Sbjct: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180
QKRVEYMETTPSVPLLSHIRIVSFL FLF+LDSLFFYNSIE LI+TRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLSFLFILDSLFFYNSIEYLIRTRKASVSLFFSFEYM 180
Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
ILATTAVSTFVKYIFYVSDVLMEGQWE+KPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANQQ--GTT 360
VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGH G DANQQ GTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHHG-SHDANQQGTGTT 360
Query: 361 NSSTQGSSGTGVAGDNLTHHQARVRAAAAAASIYEKSYVYPFASTLVWSPGYAVVPQVQR 420
+SS Q SSGTGVAGDNL+HHQARV+AAAAAASIYEKSYVYP ASTLVWSPGYAVVPQ QR
Sbjct: 361 SSSAQDSSGTGVAGDNLSHHQARVQAAAAAASIYEKSYVYPCASTLVWSPGYAVVPQTQR 420
Query: 421 PLPDSNNIESSGERAPNEQPQYFPFTGGPANLSFTQIPQNLFVPFQPREGTVNHTDGLGS 480
PL DSNNIE +GE+APN QPQYFPFTGGPANLSFTQ PQNLFVPFQPREG+VN+TDGLGS
Sbjct: 421 PLADSNNIEPNGEQAPNGQPQYFPFTGGPANLSFTQFPQNLFVPFQPREGSVNNTDGLGS 480
Query: 481 SQNISGSQLEAQERFLEQQIEILKDQLQLLRKPKVEESSRAAGEADKKGKSVASSSPSSS 540
SQN+SG LE QERF++QQIE+L+ QLQLLR PKVEES+R AGEADKKGKSVASS PSSS
Sbjct: 481 SQNVSGLHLEDQERFIQQQIEMLQKQLQLLRNPKVEESTR-AGEADKKGKSVASSPPSSS 540
Query: 541 SSVSDSQAP-GEIERM 554
SSVS+S P GEIERM
Sbjct: 541 SSVSESHPPAGEIERM 554
BLAST of Lag0019798 vs. ExPASy TrEMBL
Match:
A0A5A7V9K3 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G001010 PE=4 SV=1)
HSP 1 Score: 947.6 bits (2448), Expect = 2.9e-272
Identity = 501/559 (89.62%), Postives = 526/559 (94.10%), Query Frame = 0
Query: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
MMKLQTYAGISFIATI+VIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL
Sbjct: 1 MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
Query: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA
Sbjct: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180
QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSI+SLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180
Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANQQ--GTT 360
VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSST GH G+QSDANQQ GTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTSGHHGVQSDANQQGTGTT 360
Query: 361 NSSTQGSSGTGVAGDNLTHHQARVRAAAAAASIYEKSYVYPFASTLVWSPGYAVVPQVQR 420
+SS QG+SGTGV DNL+HHQARVRAAAAAASIYEKSYVYP ASTLVWS GYAVVPQV+R
Sbjct: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVWSLGYAVVPQVER 420
Query: 421 PLPDSNNIESSGERAPNEQPQYFPFTGGPANLSFTQIPQNLFVPFQPREGTVNHTDGLGS 480
PL DSNN E++GE++PN PQYF F GGP+NLSFTQ PQN+FVPFQPREGT N+ + LGS
Sbjct: 421 PLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPREGTGNNIEELGS 480
Query: 481 SQNISGSQLEAQERFLEQQIEILKDQLQLLRKPKVEESSRAAGEADKKGKSVA-SSSPSS 540
S N+SGSQLEAQE+FL+QQIE L++QLQLLRKPKVEESSR A E DKKGKS+A S +PS+
Sbjct: 481 SLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASETDKKGKSLAISPAPSA 540
Query: 541 SSSVSDSQAPGEIERMPAL 557
S S SDS PGE ER+ A+
Sbjct: 541 SLSSSDSHPPGETERVKAV 559
BLAST of Lag0019798 vs. TAIR 10
Match:
AT3G16090.1 (RING/U-box superfamily protein )
HSP 1 Score: 609.4 bits (1570), Expect = 3.6e-174
Identity = 339/537 (63.13%), Postives = 403/537 (75.05%), Query Frame = 0
Query: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
M++L+TYAG+SF+AT+ VIYHAF+SRGQFYPA VYLSTSKI+LVLLLNM LV+M LW L
Sbjct: 1 MIRLRTYAGLSFMATLAVIYHAFSSRGQFYPATVYLSTSKISLVLLLNMCLVLMLSLWHL 60
Query: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
K +FLGSLREAEVERLNEQ+W+ELMEILFAITIFRQDFS FL +V LLLIKALHWLA
Sbjct: 61 VKFVFLGSLREAEVERLNEQAWRELMEILFAITIFRQDFSSGFLPLVVTLLLIKALHWLA 120
Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180
QKRVEY+ETTPSV LSH RIVSF+GFL ++DSLF Y+SI LIQ+R+ASVSLFFSFEYM
Sbjct: 121 QKRVEYIETTPSVSKLSHFRIVSFMGFLLLVDSLFMYSSIRHLIQSRQASVSLFFSFEYM 180
Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
ILATT V+ FVKY+FYV+D+LM+GQWE+KPVYTFYLELIRDLLHLSMY+CFF VIFM YG
Sbjct: 181 ILATTTVAIFVKYVFYVTDMLMDGQWEKKPVYTFYLELIRDLLHLSMYICFFFVIFMNYG 240
Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
VPLHL+RELYE FRNF+IR++DY+RYRKITSNMNDRFPDATPEEL +SDATCIICREEMT
Sbjct: 241 VPLHLLRELYETFRNFQIRVSDYLRYRKITSNMNDRFPDATPEELTASDATCIICREEMT 300
Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANQQGTTNS 360
AKKL+CGHLFHVHCLRSWLERQ TCPTCRALVVPPE +++A Q +QQGT++S
Sbjct: 301 NAKKLICGHLFHVHCLRSWLERQQTCPTCRALVVPPENATSAAPGQRELHQGSQQGTSSS 360
Query: 361 STQG---SSGTGVAGDNLTHHQARVRAAAAAASIYEKSYVYPFASTLVWSPGYAVVPQVQ 420
QG SS GV+ ++L+ H AR++AAA+AAS+Y KS VYP A+T+ WS G VP
Sbjct: 361 GNQGSEISSSAGVSNNSLSRHHARLQAAASAASVYGKSMVYPSANTVAWSSG---VP--- 420
Query: 421 RPLPDSNNIESSGERAPNEQPQYFPFTGGPANLSFTQIPQNLFVPFQPREGTVNHTDGLG 480
E+ E Q P P S
Sbjct: 421 -----------GTEQVSTEPDQTLPQHNLPVENSH------------------------- 480
Query: 481 SSQNISGSQLEAQERFLEQQIEILKDQLQLLRKPKVEESSRAAGEADKKGKSVASSS 535
+ N+S ++LE + LE +EIL+++L L K E +AGE + KGKSVA ++
Sbjct: 481 AYANMSETKLEEMRKSLETHLEILRNRLHFLETRKPE----SAGEPENKGKSVADAA 491
BLAST of Lag0019798 vs. TAIR 10
Match:
AT1G65040.2 (RING/U-box superfamily protein )
HSP 1 Score: 601.3 bits (1549), Expect = 9.7e-172
Identity = 335/538 (62.27%), Postives = 397/538 (73.79%), Query Frame = 0
Query: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
M++L+ YAG+S +AT+VVIYHAF+SRGQFYPA VYLSTSKINLV+LLNMGLV+M LW L
Sbjct: 1 MIQLKVYAGLSTLATLVVIYHAFSSRGQFYPATVYLSTSKINLVVLLNMGLVLMLSLWNL 60
Query: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
K +FLGSLREAEVERLNEQ+W+ELMEILFAITIFRQDFSV F+ +V LLLIK LHW+A
Sbjct: 61 VKIVFLGSLREAEVERLNEQAWRELMEILFAITIFRQDFSVGFISLVVTLLLIKGLHWMA 120
Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180
QKRVEY+ETTPSV LLSH+RIVSF+ FL +LD L Y+SI+ LIQ+RKAS+S+FF+FEYM
Sbjct: 121 QKRVEYIETTPSVTLLSHVRIVSFMVFLLILDCLLTYSSIQQLIQSRKASMSVFFTFEYM 180
Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
ILATT VS VKY FYV+D+L EGQWE KPVYTFYLEL+RDLLHLSMYLCFFL+IFM YG
Sbjct: 181 ILATTTVSIIVKYAFYVTDMLKEGQWEGKPVYTFYLELVRDLLHLSMYLCFFLMIFMNYG 240
Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
+PLHLIRELYE FRNF+IR+ DY+RYRKITSNMNDRFPDATPEEL+S+DATCIICREEMT
Sbjct: 241 LPLHLIRELYETFRNFKIRVTDYLRYRKITSNMNDRFPDATPEELSSNDATCIICREEMT 300
Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPE-GSSTAGGHQGLQSDANQQGTTN 360
AKKLVCGHLFHVHCLRSWLERQ+TCPTCRALVVP E +STA G++G ++ QQGT
Sbjct: 301 SAKKLVCGHLFHVHCLRSWLERQNTCPTCRALVVPAENATSTASGNRGPHQESLQQGTGT 360
Query: 361 SST--QGSSGTGVAGDNLTHHQARVRAAAAAASIYEKSYVYP-FASTLVWSPGYAVVPQV 420
SS+ QGSS + A +N++ H+AR +AAA+AASIY +S VYP A+TLVWS G +++PQ
Sbjct: 361 SSSDGQGSSVSAAASENMSRHEARFQAAASAASIYGRSIVYPSSANTLVWSQGNSLLPQ- 420
Query: 421 QRPLPDSNNIESSGERAPNEQPQYFPFTGGPANLSFTQIPQNLFVPFQPREGTVNHTDGL 480
Sbjct: 421 ------------------------------------------------------------ 460
Query: 481 GSSQNISGSQLEAQERFLEQQIEILKDQLQLLRKPKVEESSRAAGEADKKGKSVASSS 535
+++EAQ RFLE QIE+L +QL+LL KP D KGKSVA ++
Sbjct: 481 --------TEVEAQRRFLESQIEVLTNQLRLLEKPTT---------VDTKGKSVADTA 460
BLAST of Lag0019798 vs. TAIR 10
Match:
AT1G65040.3 (RING/U-box superfamily protein )
HSP 1 Score: 468.8 bits (1205), Expect = 7.5e-132
Identity = 280/538 (52.04%), Postives = 335/538 (62.27%), Query Frame = 0
Query: 1 MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60
M++L+ YAG+S +AT+VVIYHAF+SRGQFYPA VYLSTSKINL
Sbjct: 1 MIQLKVYAGLSTLATLVVIYHAFSSRGQFYPATVYLSTSKINL----------------- 60
Query: 61 TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120
LHW+A
Sbjct: 61 ------------------------------------------------------GLHWMA 120
Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIESLIQTRKASVSLFFSFEYM 180
QKRVEY+ETTPSV LLSH+RIVSF+ FL +LD L Y+SI+ LIQ+RKAS+S+FF+FEYM
Sbjct: 121 QKRVEYIETTPSVTLLSHVRIVSFMVFLLILDCLLTYSSIQQLIQSRKASMSVFFTFEYM 180
Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
ILATT VS VKY FYV+D+L EGQWE KPVYTFYLEL+RDLLHLSMYLCFFL+IFM YG
Sbjct: 181 ILATTTVSIIVKYAFYVTDMLKEGQWEGKPVYTFYLELVRDLLHLSMYLCFFLMIFMNYG 240
Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
+PLHLIRELYE FRNF+IR+ DY+RYRKITSNMNDRFPDATPEEL+S+DATCIICREEMT
Sbjct: 241 LPLHLIRELYETFRNFKIRVTDYLRYRKITSNMNDRFPDATPEELSSNDATCIICREEMT 300
Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPE-GSSTAGGHQGLQSDANQQGTTN 360
AKKLVCGHLFHVHCLRSWLERQ+TCPTCRALVVP E +STA G++G ++ QQGT
Sbjct: 301 SAKKLVCGHLFHVHCLRSWLERQNTCPTCRALVVPAENATSTASGNRGPHQESLQQGTGT 360
Query: 361 SST--QGSSGTGVAGDNLTHHQARVRAAAAAASIYEKSYVYP-FASTLVWSPGYAVVPQV 420
SS+ QGSS + A +N++ H+AR +AAA+AASIY +S VYP A+TLVWS G +++PQ
Sbjct: 361 SSSDGQGSSVSAAASENMSRHEARFQAAASAASIYGRSIVYPSSANTLVWSQGNSLLPQ- 389
Query: 421 QRPLPDSNNIESSGERAPNEQPQYFPFTGGPANLSFTQIPQNLFVPFQPREGTVNHTDGL 480
Sbjct: 421 ------------------------------------------------------------ 389
Query: 481 GSSQNISGSQLEAQERFLEQQIEILKDQLQLLRKPKVEESSRAAGEADKKGKSVASSS 535
+++EAQ RFLE QIE+L +QL+LL KP D KGKSVA ++
Sbjct: 481 --------TEVEAQRRFLESQIEVLTNQLRLLEKPTT---------VDTKGKSVADTA 389
BLAST of Lag0019798 vs. TAIR 10
Match:
AT1G65040.1 (RING/U-box superfamily protein )
HSP 1 Score: 344.7 bits (883), Expect = 1.6e-94
Identity = 201/359 (55.99%), Postives = 238/359 (66.30%), Query Frame = 0
Query: 180 MILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTY 239
MILATT VS VKY FYV+D+L EGQWE KPVYTFYLEL+RDLLHLSMYLCFFL+IFM Y
Sbjct: 1 MILATTTVSIIVKYAFYVTDMLKEGQWEGKPVYTFYLELVRDLLHLSMYLCFFLMIFMNY 60
Query: 240 GVPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEM 299
G+PLHLIRELYE FRNF+IR+ DY+RYRKITSNMNDRFPDATPEEL+S+DATCIICREEM
Sbjct: 61 GLPLHLIRELYETFRNFKIRVTDYLRYRKITSNMNDRFPDATPEELSSNDATCIICREEM 120
Query: 300 TVAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPE-GSSTAGGHQGLQSDANQQGTT 359
T AKKLVCGHLFHVHCLRSWLERQ+TCPTCRALVVP E +STA G++G ++ QQGT
Sbjct: 121 TSAKKLVCGHLFHVHCLRSWLERQNTCPTCRALVVPAENATSTASGNRGPHQESLQQGTG 180
Query: 360 NSST--QGSSGTGVAGDNLTHHQARVRAAAAAASIYEKSYVYP-FASTLVWSPGYAVVPQ 419
SS+ QGSS + A +N++ H+AR +AAA+AASIY +S VYP A+TLVWS G +++PQ
Sbjct: 181 TSSSDGQGSSVSAAASENMSRHEARFQAAASAASIYGRSIVYPSSANTLVWSQGNSLLPQ 240
Query: 420 VQRPLPDSNNIESSGERAPNEQPQYFPFTGGPANLSFTQIPQNLFVPFQPREGTVNHTDG 479
Sbjct: 241 ------------------------------------------------------------ 281
Query: 480 LGSSQNISGSQLEAQERFLEQQIEILKDQLQLLRKPKVEESSRAAGEADKKGKSVASSS 535
+++EAQ RFLE QIE+L +QL+LL KP D KGKSVA ++
Sbjct: 301 ---------TEVEAQRRFLESQIEVLTNQLRLLEKPTT---------VDTKGKSVADTA 281
BLAST of Lag0019798 vs. TAIR 10
Match:
AT4G25230.1 (RPM1 interacting protein 2 )
HSP 1 Score: 94.7 bits (234), Expect = 2.9e-19
Identity = 97/343 (28.28%), Postives = 153/343 (44.61%), Query Frame = 0
Query: 25 SRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQLTKKIFLGSLREAEVERLNEQSWKE 84
S G AL L S + LL N L + +L K +F G L + E ++L E+
Sbjct: 42 SLGDSEHALELLLGSYFTIALLTNFVLNVYILLVLSLKTLFFGDLYDVETKKLVERLANY 101
Query: 85 L------MEILFAITIFRQDFSVAFLGMVTALLLIKALHWLAQKRVEYMETTPSVPLLSH 144
+ + ++ TIF+ V + +T L +K LA+ R+E + +PS ++
Sbjct: 102 IIYKGTFLPLVIPPTIFQ---GVLWTVWLTVLCTLKMFQALARDRLERLNASPSSTPWTY 161
Query: 145 IRIVSFLGFLFVLD------SLFFYNSIESLIQTRKASVSLFFSFE----YMILATTAVS 204
R+ S L + +D SL YN+I S + +FE +I +
Sbjct: 162 FRVYSVLFLVLSVDMFWIKLSLMTYNTIGSAVYLLLLFEPCSIAFETLQALLIHGFQLLD 221
Query: 205 TFVKYIF----------YVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMT 264
++ ++ ++ + E K + L D+ L M L +L I+
Sbjct: 222 MWINHLAVKNSDCQRSKFIDSMTAGSLLEWKGLLNRNLGFFLDMATLVMALGHYLHIWWL 281
Query: 265 YGVPLHLIRELYEIFRNFRI-------RIADYIRYRKITSNMNDRFPDATPEELNSSDAT 324
+G+ HL+ + +F N R RI YI+ R ++ PDAT EEL + D
Sbjct: 282 HGIAFHLVDAV--LFLNIRALLSAILKRIKGYIKLRIALGALHAALPDATSEELRAYDDE 341
Query: 325 CIICREEMTVAKKLVCGHLFHVHCLRSWLER----QHTCPTCR 331
C ICRE M AK+L C HLFH+ CLRSWL++ ++CPTCR
Sbjct: 342 CAICREPMAKAKRLHCNHLFHLGCLRSWLDQGLNEVYSCPTCR 379
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
KAG6600225.1 | 1.8e-281 | 89.63 | ERAD-associated E3 ubiquitin-protein ligase HRD1A, partial [Cucurbita argyrosper... | [more] |
KAG7030884.1 | 3.5e-280 | 93.32 | ERAD-associated E3 ubiquitin-protein ligase HRD1A [Cucurbita argyrosperma subsp.... | [more] |
XP_023531768.1 | 6.5e-279 | 92.96 | ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita pepo subsp. pe... | [more] |
XP_022942315.1 | 1.1e-278 | 92.78 | ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita moschata] >XP_... | [more] |
XP_022141279.1 | 4.2e-278 | 92.60 | ERAD-associated E3 ubiquitin-protein ligase HRD1B-like isoform X2 [Momordica cha... | [more] |
Match Name | E-value | Identity | Description | |
Q9LW77 | 5.0e-173 | 63.13 | ERAD-associated E3 ubiquitin-protein ligase HRD1A OS=Arabidopsis thaliana OX=370... | [more] |
Q6NPT7 | 1.4e-170 | 62.27 | ERAD-associated E3 ubiquitin-protein ligase HRD1B OS=Arabidopsis thaliana OX=370... | [more] |
Q5Z880 | 1.1e-159 | 60.51 | ERAD-associated E3 ubiquitin-protein ligase HRD1 OS=Oryza sativa subsp. japonica... | [more] |
Q803I8 | 5.2e-69 | 42.60 | E3 ubiquitin-protein ligase synoviolin OS=Danio rerio OX=7955 GN=syvn1 PE=2 SV=2 | [more] |
Q86TM6 | 2.6e-68 | 42.60 | E3 ubiquitin-protein ligase synoviolin OS=Homo sapiens OX=9606 GN=SYVN1 PE=1 SV=... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1FNJ3 | 5.4e-279 | 92.78 | ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucurbita moschata OX=... | [more] |
A0A6J1CI55 | 2.1e-278 | 92.60 | ERAD-associated E3 ubiquitin-protein ligase HRD1B-like isoform X2 OS=Momordica c... | [more] |
A0A6J1KWZ5 | 2.7e-278 | 92.60 | ERAD-associated E3 ubiquitin-protein ligase HRD1B-like isoform X2 OS=Cucurbita m... | [more] |
A0A6J1CJF5 | 6.6e-277 | 92.27 | ERAD-associated E3 ubiquitin-protein ligase HRD1B-like isoform X1 OS=Momordica c... | [more] |
A0A5A7V9K3 | 2.9e-272 | 89.62 | ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucumis melo var. maku... | [more] |