Lag0039782 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0039782 vs. NCBI nr
Match: XP_022155217.1 (hydrolase tropI [Momordica charantia])

HSP 1 Score: 445.3 bits (1144), Expect = 4.4e-121
Identity = 216/267 (80.90%), Postives = 237/267 (88.76%), Query Frame = 0

Query: 47  KRERKGGKVCYSYSLQGGVKKDEIIIDDDDDCELVNGMELSIGEEKPEPHHTIPAYLFKP 106
           KRE+KGG+VC S+ L+G +         D+ CELV+GMELSIGE   +P  T+PA+LFK 
Sbjct: 39  KREKKGGQVCSSHRLEGEI---------DEGCELVSGMELSIGE---QPDRTVPAHLFKA 98

Query: 107 LKNNNGTAILLLSDLFAFQHSSTTDFAYRLACNGFNVLLPDLFHGDPWKKKQPVEQSTKQ 166
           +KNNNGTAILLLSDLFAFQ SS TDFAYR+ACNGFNVLLPD+FHGDPWKKKQPVEQ+ KQ
Sbjct: 99  VKNNNGTAILLLSDLFAFQDSSITDFAYRVACNGFNVLLPDVFHGDPWKKKQPVEQTAKQ 158

Query: 167 DMQRIAEDIGRATKWLVDEFSAAGISRKLGIMGFGYGGGQVIDVLARDQGACFSIGASFY 226
           DMQR+AEDIG ATKWLV+EFSAAGIS KLGIMGFGYGGGQVIDVLARDQGACF+IGASFY
Sbjct: 159 DMQRVAEDIGTATKWLVEEFSAAGISTKLGIMGFGYGGGQVIDVLARDQGACFAIGASFY 218

Query: 227 GTKVDTSLASKVKVPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPG 286
           GT +D SL S+VKVPVLLISGDNDPLCPVSVMKDLEKG+G+GSKLVIFGGRGHGF HKPG
Sbjct: 219 GTNIDPSLGSQVKVPVLLISGDNDPLCPVSVMKDLEKGIGKGSKLVIFGGRGHGFFHKPG 278

Query: 287 SPEEDEDAEHAFLMLRNWLHDGLVATN 314
           SPEEDEDAE AFLMLRNWLHDGLV TN
Sbjct: 279 SPEEDEDAERAFLMLRNWLHDGLVVTN 293

BLAST of Lag0039782 vs. NCBI nr
Match: XP_038890567.1 (hydrolase tropI [Benincasa hispida])

HSP 1 Score: 440.7 bits (1132), Expect = 1.1e-119
Identity = 240/324 (74.07%), Postives = 258/324 (79.63%), Query Frame = 0

Query: 1   MELASAGAQLISRLPTPTGTGTGTGSSGI--------ISTLTPFLK---GSVYLKSSKRE 60
           M LASAGAQLIS LP PTG+ +     G          ++  PF K    S +    KRE
Sbjct: 1   MGLASAGAQLIS-LP-PTGSSSCRRRIGTPFQLPYFRFTSPLPFRKCGNRSDWKWKGKRE 60

Query: 61  RKGGKVCYSYSLQGGVKKDEIIIDDDDDCELVNGMELSIGEEKPEPHHTIPAYLFKPLKN 120
           +KGGKVCYS     GV+ D+   DDD  CELVNGMELSIGE   +PHH+IPAYLFK +KN
Sbjct: 61  KKGGKVCYSVE---GVEDDD---DDDAACELVNGMELSIGE---QPHHSIPAYLFKAVKN 120

Query: 121 NNGTAILLLSDLFAFQHSSTTDFAYRLACNGFNVLLPDLFHGDPWKKKQPVEQSTKQDMQ 180
           NNGTAILLLSDLFAFQHSS  DFAYRLACNGFNVLLPDL HGD WKK     QSTK+DMQ
Sbjct: 121 NNGTAILLLSDLFAFQHSSIRDFAYRLACNGFNVLLPDLIHGDSWKK-----QSTKRDMQ 180

Query: 181 RIAEDIGRATKWLVDEFSAAGISRKLGIMGFGYGGGQVIDVLARDQGACFSIGASFYGTK 240
           RIAEDIG  TKWLVDEFSAAG+SRKLGIMGFGYGGGQVI+VLARD+GACF IGAS YGT+
Sbjct: 181 RIAEDIGTVTKWLVDEFSAAGLSRKLGIMGFGYGGGQVIEVLARDEGACFCIGASLYGTE 240

Query: 241 VDTSLASKVKVPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPE 300
           VD SLA+KVKVPVLLISGDNDPLCPVSVMKDLEK MGEGSKLVIFGGRGH FVH P SPE
Sbjct: 241 VDPSLATKVKVPVLLISGDNDPLCPVSVMKDLEKRMGEGSKLVIFGGRGHDFVHNPRSPE 300

Query: 301 EDEDAEHAFLMLRNWLHDGLVATN 314
           EDEDAE AFLM+RNWLH GLVATN
Sbjct: 301 EDEDAEQAFLMIRNWLHHGLVATN 308

BLAST of Lag0039782 vs. NCBI nr
Match: XP_008459689.1 (PREDICTED: uncharacterized protein LOC103498731 [Cucumis melo] >KAA0039216.1 carboxymethylenebutenolidase-like protein [Cucumis melo var. makuwa] >TYK00404.1 carboxymethylenebutenolidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 420.6 bits (1080), Expect = 1.2e-113
Identity = 228/319 (71.47%), Postives = 250/319 (78.37%), Query Frame = 0

Query: 1   MELASAGAQLISRLPTPTGTGTGTGSSGIISTLTPFLKGSVY-LKSSKRERKG-----GK 60
           M  ASAGAQLIS  P      TG+ S  +   + P      Y L S K    G     GK
Sbjct: 1   MGFASAGAQLISLPP------TGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGK 60

Query: 61  VCYSYSLQGGVKKDEIIIDDDDDCELVNGMELSIGEEKPEPHHTIPAYLFKPLKNNNGTA 120
           + Y YS++G VK      DDDD CELVNGMELSIGE   +PHH+IPAYLFK +KNNNGTA
Sbjct: 61  LRY-YSVKGRVKD-----DDDDACELVNGMELSIGE---QPHHSIPAYLFKAVKNNNGTA 120

Query: 121 ILLLSDLFAFQHSSTTDFAYRLACNGFNVLLPDLFHGDPWKKKQPVEQSTKQDMQRIAED 180
           ILLLSDLFAFQ SS  DFAYRLACNGFNVLLPDL + DPW +K P + S KQ+MQRIAED
Sbjct: 121 ILLLSDLFAFQDSSIRDFAYRLACNGFNVLLPDLVNEDPW-EKPPAQHSAKQEMQRIAED 180

Query: 181 IGRATKWLVDEFSAAGISRKLGIMGFGYGGGQVIDVLARDQGACFSIGASFYGTKVDTSL 240
           IG  TKWL DEFSAAG+SRKLGI+GFG+GGG+VIDVLARD+GACF IGAS YGT+VD SL
Sbjct: 181 IGTVTKWLTDEFSAAGLSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVDPSL 240

Query: 241 ASKVKVPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDA 300
           A+KVKVPVLLISGD+DPLCPVSVMKDLEKGMGEGSKLVIF GRGHGFVH PGSPEED+DA
Sbjct: 241 ATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDA 300

Query: 301 EHAFLMLRNWLHDGLVATN 314
           E AFLMLRNWLH GLVATN
Sbjct: 301 EQAFLMLRNWLHHGLVATN 303

BLAST of Lag0039782 vs. NCBI nr
Match: XP_004141569.1 (uncharacterized protein LOC101210518 [Cucumis sativus] >KGN52723.1 hypothetical protein Csa_008898 [Cucumis sativus])

HSP 1 Score: 418.3 bits (1074), Expect = 5.8e-113
Identity = 225/319 (70.53%), Postives = 247/319 (77.43%), Query Frame = 0

Query: 1   MELASAGAQLISRLPTPTGTGTGTGSSGIISTL-TPFLKGSVYLKSSKRERKG-----GK 60
           M  ASAGAQLIS  P      TG+ S  +      P+ K +  L S K    G     GK
Sbjct: 1   MGFASAGAQLISLPP------TGSSSRRVGKPFQPPYFKFAYPLPSRKYSNMGVWRWKGK 60

Query: 61  VCYSYSLQGGVKKDEIIIDDDDDCELVNGMELSIGEEKPEPHHTIPAYLFKPLKNNNGTA 120
           +   YS++G +K D    DDDD CELVNGMELSIGE   +PHH+IPAYLFK +KNNNGTA
Sbjct: 61  L--RYSVEGRMKDD----DDDDACELVNGMELSIGE---QPHHSIPAYLFKAVKNNNGTA 120

Query: 121 ILLLSDLFAFQHSSTTDFAYRLACNGFNVLLPDLFHGDPWKKKQPVEQSTKQDMQRIAED 180
           ILLLSDLFAFQHSS  DFAYRLAC GFNVLLPDL H D W +K P E   KQ+MQRIAED
Sbjct: 121 ILLLSDLFAFQHSSIRDFAYRLACIGFNVLLPDLVHEDVW-EKPPAEHCAKQEMQRIAED 180

Query: 181 IGRATKWLVDEFSAAGISRKLGIMGFGYGGGQVIDVLARDQGACFSIGASFYGTKVDTSL 240
           IG  TKWL +EFSAAG+S KLGI+GFGYGGG+VIDVLARD+G CF IGAS YGT+VD SL
Sbjct: 181 IGTVTKWLTEEFSAAGLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSL 240

Query: 241 ASKVKVPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDA 300
           A+KVKVPVLLISGDNDPLCPVSVMKDLEKG+GEGSKLVIFGGRGHGFVH PGSPEED+DA
Sbjct: 241 ATKVKVPVLLISGDNDPLCPVSVMKDLEKGIGEGSKLVIFGGRGHGFVHNPGSPEEDDDA 300

Query: 301 EHAFLMLRNWLHDGLVATN 314
           E AFLMLRNWLH GLVA N
Sbjct: 301 EKAFLMLRNWLHHGLVAIN 303

BLAST of Lag0039782 vs. NCBI nr
Match: XP_023536049.1 (carboxymethylenebutenolidase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 406.0 bits (1042), Expect = 3.0e-109
Identity = 221/314 (70.38%), Postives = 243/314 (77.39%), Query Frame = 0

Query: 1   MELASAGAQLISRLPT-PTGTGTGTGSSGIISTLTPFLKGSVYLKSSKRERKGGKVCYSY 60
           M  A+AGAQ IS  PT  +G   G           P       LK  KRE+KGGK+CYS 
Sbjct: 1   MGSATAGAQWISAPPTGRSGRRMGIPFQPSFRFAFPISNNIGQLK-GKREKKGGKLCYSA 60

Query: 61  SLQGGVKKDEIIIDDDDDCELVNGMELSIGEEKPEPHHTIPAYLFKPLKNNNGTAILLLS 120
               GVK +   ID+++ CELVNGMELSIGE   E HH+I AYLFK +KNNNGTAILLL+
Sbjct: 61  E---GVKDE---IDEEEACELVNGMELSIGE---EAHHSITAYLFKAVKNNNGTAILLLT 120

Query: 121 DLFAFQHSSTTDFAYRLACNGFNVLLPDLFHGDPWKKKQPVEQSTKQDMQRIAEDIGRAT 180
           DLFAFQ SS  DFAYRLACNGFNVLLP++FHGD              + Q+I EDIG AT
Sbjct: 121 DLFAFQDSSIRDFAYRLACNGFNVLLPNIFHGD------------SSEEQKIVEDIGAAT 180

Query: 181 KWLVDEFSAAGISRKLGIMGFGYGGGQVIDVLARDQGACFSIGASFYGTKVDTSLASKVK 240
           KWLVDEFSAAGISRKLGI+GFGYGGG+VIDVLARDQG+ FSIGAS YGT+VD SLA+KV+
Sbjct: 181 KWLVDEFSAAGISRKLGIVGFGYGGGRVIDVLARDQGSYFSIGASLYGTEVDPSLATKVE 240

Query: 241 VPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEHAFL 300
           VPVLLISGDND LCPVSVMKDLEKGMG+GSKLVIFGGRGHGFVHKPGSPEEDEDAE AFL
Sbjct: 241 VPVLLISGDNDTLCPVSVMKDLEKGMGDGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFL 292

Query: 301 MLRNWLHDGLVATN 314
           MLRNWLHDGLVATN
Sbjct: 301 MLRNWLHDGLVATN 292

BLAST of Lag0039782 vs. ExPASy Swiss-Prot
Match: Q7TP52 (Carboxymethylenebutenolidase homolog OS=Rattus norvegicus OX=10116 GN=Cmbl PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 9.3e-13
Identity = 58/203 (28.57%), Postives = 93/203 (45.81%), Query Frame = 0

Query: 112 GTAILLLSDLFAFQHSSTTDFAYRLACNGFNVLLPDLFHG-DPWKKKQPVEQSTKQDMQR 171
           G A++++ D+F +Q S+T   A  +A NG+  ++PD F G +PW          +    R
Sbjct: 42  GKAVIVVQDIFGWQLSNTRYMADMIAGNGYTTIVPDFFVGQEPWDPAGDWSTFPEWLKSR 101

Query: 172 IAEDIGRATKWLVDEFSAAGISRKLGIMGFGYGGGQVIDVLARDQGACFSIGASFYGTKV 231
            A  I R    ++        ++K+GI+GF +GG  V  V+          G S YG   
Sbjct: 102 NARKINREVDAVLRYLKQQCHAQKIGIVGFCWGGIVVHHVMTTYPEV--RAGVSVYGIIR 161

Query: 232 DTSLASKVKVPVLLISGDNDPLCPVSVMKDLEKGMGE----GSKLVIFGGRGHGFVHKPG 291
           D+     +K P L I  +ND + P+  +  L + + E      ++  F G+ HGFVH+  
Sbjct: 162 DSEDVYNLKNPTLFIFAENDAVIPLEQVSILIQKLKEHCIVNYQVKTFSGQTHGFVHRKR 221

Query: 292 ---SPEEDEDAEHAFLMLRNWLH 307
              SP +    E A   L  WL+
Sbjct: 222 EDCSPADKPYIEEARRNLIEWLN 242

BLAST of Lag0039782 vs. ExPASy Swiss-Prot
Match: B8M9K0 (Hydrolase tropI OS=Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) OX=441959 GN=tropI PE=3 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 3.4e-07
Identity = 43/195 (22.05%), Postives = 86/195 (44.10%), Query Frame = 0

Query: 99  IPAYLFKPLKNNNGTAILLLSDLFAFQHSSTTDFAYRLACNGFNVLLPDLFHGDPWKKKQ 158
           I  Y   P   +    +L+L+D+   + S+    A + A NG+ V++PDLF GD     +
Sbjct: 30  IEVYTSYPPDKSTEYGVLILTDIVGHRFSNAQIIADQFAENGYFVMMPDLFLGDAVPLNK 89

Query: 159 PVEQST---------KQDMQRIAEDIGRATKWLVDEFSAAGISRKLGIMGFGYGGGQVID 218
           P E             Q    + E +    +  + E  +    +K+G +G+ +GG  V+ 
Sbjct: 90  PGEFDMGKWRSGAYHPQGKNHLPETVDPIVEVCLSEMRSKYQCKKIGAVGYCFGGKYVVR 149

Query: 219 VLARDQGACFSIGASFYGTKVDTSLASKVKVPVLLISGDNDPLCPVS---VMKDLEKGMG 278
            L   +     +G + + + +D S    +K P+ + +   D + P     V +++ + +G
Sbjct: 150 HLIPGK---MDVGYTAHPSHIDESELKGIKGPLAIAAAAKDNIFPAEKRHVSEEILQEVG 209

Query: 279 EGSKLVIFGGRGHGF 282
              ++ ++ G  HGF
Sbjct: 210 FPYQINLYSGVSHGF 221

BLAST of Lag0039782 vs. ExPASy Swiss-Prot
Match: A0A2V5GSC6 (Hydrolase pyvD OS=Aspergillus violaceofuscus (strain CBS 115571) OX=1450538 GN=pyvD PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 3.8e-06
Identity = 51/191 (26.70%), Postives = 83/191 (43.46%), Query Frame = 0

Query: 99  IPAYLFKPLKNNNGTAILLLSDLFAFQHSSTTDFAYRLACNGFNVLLPDLFHGDPWKKKQ 158
           IP Y+  P   +   AIL LSD+F     ++   A   A NG+ V++PDLF GDP     
Sbjct: 30  IPVYVSYPPDKSTHNAILFLSDIFGLALVNSKLIADLFAANGYLVVMPDLFQGDP----V 89

Query: 159 PVEQSTK-QDMQRIAEDIGRATKWLVD----EFSAAGISRKLGIMGFGYGGGQVIDVLAR 218
           PV+ S   Q M  +   +   T  +V     E       +++G +G+ YGG  V+  L  
Sbjct: 90  PVDHSPSFQIMDWLQGHLPPQTDPIVTATLREMRERLGCQRIGGVGYCYGGKYVVRYLHP 149

Query: 219 DQGACFSIGASFYGTKVDTSLASKVKVPVLLISGDNDPLCPVSVMKDLEKGMGE---GSK 278
            Q     +G   + T ++      ++ P+ + +   D L P     + E  + +     +
Sbjct: 150 GQ---LDVGFVAHPTFIEPDELKAIEGPLSISASAQDNLFPPEKRHESETILAQLDVPYQ 209

Query: 279 LVIFGGRGHGF 282
           + IF    HGF
Sbjct: 210 INIFSDVEHGF 213

BLAST of Lag0039782 vs. ExPASy TrEMBL
Match: A0A6J1DNR6 (hydrolase tropI OS=Momordica charantia OX=3673 GN=LOC111022362 PE=4 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 2.1e-121
Identity = 216/267 (80.90%), Postives = 237/267 (88.76%), Query Frame = 0

Query: 47  KRERKGGKVCYSYSLQGGVKKDEIIIDDDDDCELVNGMELSIGEEKPEPHHTIPAYLFKP 106
           KRE+KGG+VC S+ L+G +         D+ CELV+GMELSIGE   +P  T+PA+LFK 
Sbjct: 39  KREKKGGQVCSSHRLEGEI---------DEGCELVSGMELSIGE---QPDRTVPAHLFKA 98

Query: 107 LKNNNGTAILLLSDLFAFQHSSTTDFAYRLACNGFNVLLPDLFHGDPWKKKQPVEQSTKQ 166
           +KNNNGTAILLLSDLFAFQ SS TDFAYR+ACNGFNVLLPD+FHGDPWKKKQPVEQ+ KQ
Sbjct: 99  VKNNNGTAILLLSDLFAFQDSSITDFAYRVACNGFNVLLPDVFHGDPWKKKQPVEQTAKQ 158

Query: 167 DMQRIAEDIGRATKWLVDEFSAAGISRKLGIMGFGYGGGQVIDVLARDQGACFSIGASFY 226
           DMQR+AEDIG ATKWLV+EFSAAGIS KLGIMGFGYGGGQVIDVLARDQGACF+IGASFY
Sbjct: 159 DMQRVAEDIGTATKWLVEEFSAAGISTKLGIMGFGYGGGQVIDVLARDQGACFAIGASFY 218

Query: 227 GTKVDTSLASKVKVPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPG 286
           GT +D SL S+VKVPVLLISGDNDPLCPVSVMKDLEKG+G+GSKLVIFGGRGHGF HKPG
Sbjct: 219 GTNIDPSLGSQVKVPVLLISGDNDPLCPVSVMKDLEKGIGKGSKLVIFGGRGHGFFHKPG 278

Query: 287 SPEEDEDAEHAFLMLRNWLHDGLVATN 314
           SPEEDEDAE AFLMLRNWLHDGLV TN
Sbjct: 279 SPEEDEDAERAFLMLRNWLHDGLVVTN 293

BLAST of Lag0039782 vs. ExPASy TrEMBL
Match: A0A5A7TB03 (Carboxymethylenebutenolidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00030 PE=4 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 5.7e-114
Identity = 228/319 (71.47%), Postives = 250/319 (78.37%), Query Frame = 0

Query: 1   MELASAGAQLISRLPTPTGTGTGTGSSGIISTLTPFLKGSVY-LKSSKRERKG-----GK 60
           M  ASAGAQLIS  P      TG+ S  +   + P      Y L S K    G     GK
Sbjct: 1   MGFASAGAQLISLPP------TGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGK 60

Query: 61  VCYSYSLQGGVKKDEIIIDDDDDCELVNGMELSIGEEKPEPHHTIPAYLFKPLKNNNGTA 120
           + Y YS++G VK      DDDD CELVNGMELSIGE   +PHH+IPAYLFK +KNNNGTA
Sbjct: 61  LRY-YSVKGRVKD-----DDDDACELVNGMELSIGE---QPHHSIPAYLFKAVKNNNGTA 120

Query: 121 ILLLSDLFAFQHSSTTDFAYRLACNGFNVLLPDLFHGDPWKKKQPVEQSTKQDMQRIAED 180
           ILLLSDLFAFQ SS  DFAYRLACNGFNVLLPDL + DPW +K P + S KQ+MQRIAED
Sbjct: 121 ILLLSDLFAFQDSSIRDFAYRLACNGFNVLLPDLVNEDPW-EKPPAQHSAKQEMQRIAED 180

Query: 181 IGRATKWLVDEFSAAGISRKLGIMGFGYGGGQVIDVLARDQGACFSIGASFYGTKVDTSL 240
           IG  TKWL DEFSAAG+SRKLGI+GFG+GGG+VIDVLARD+GACF IGAS YGT+VD SL
Sbjct: 181 IGTVTKWLTDEFSAAGLSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVDPSL 240

Query: 241 ASKVKVPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDA 300
           A+KVKVPVLLISGD+DPLCPVSVMKDLEKGMGEGSKLVIF GRGHGFVH PGSPEED+DA
Sbjct: 241 ATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDA 300

Query: 301 EHAFLMLRNWLHDGLVATN 314
           E AFLMLRNWLH GLVATN
Sbjct: 301 EQAFLMLRNWLHHGLVATN 303

BLAST of Lag0039782 vs. ExPASy TrEMBL
Match: A0A1S3CB89 (uncharacterized protein LOC103498731 OS=Cucumis melo OX=3656 GN=LOC103498731 PE=4 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 5.7e-114
Identity = 228/319 (71.47%), Postives = 250/319 (78.37%), Query Frame = 0

Query: 1   MELASAGAQLISRLPTPTGTGTGTGSSGIISTLTPFLKGSVY-LKSSKRERKG-----GK 60
           M  ASAGAQLIS  P      TG+ S  +   + P      Y L S K    G     GK
Sbjct: 1   MGFASAGAQLISLPP------TGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGK 60

Query: 61  VCYSYSLQGGVKKDEIIIDDDDDCELVNGMELSIGEEKPEPHHTIPAYLFKPLKNNNGTA 120
           + Y YS++G VK      DDDD CELVNGMELSIGE   +PHH+IPAYLFK +KNNNGTA
Sbjct: 61  LRY-YSVKGRVKD-----DDDDACELVNGMELSIGE---QPHHSIPAYLFKAVKNNNGTA 120

Query: 121 ILLLSDLFAFQHSSTTDFAYRLACNGFNVLLPDLFHGDPWKKKQPVEQSTKQDMQRIAED 180
           ILLLSDLFAFQ SS  DFAYRLACNGFNVLLPDL + DPW +K P + S KQ+MQRIAED
Sbjct: 121 ILLLSDLFAFQDSSIRDFAYRLACNGFNVLLPDLVNEDPW-EKPPAQHSAKQEMQRIAED 180

Query: 181 IGRATKWLVDEFSAAGISRKLGIMGFGYGGGQVIDVLARDQGACFSIGASFYGTKVDTSL 240
           IG  TKWL DEFSAAG+SRKLGI+GFG+GGG+VIDVLARD+GACF IGAS YGT+VD SL
Sbjct: 181 IGTVTKWLTDEFSAAGLSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVDPSL 240

Query: 241 ASKVKVPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDA 300
           A+KVKVPVLLISGD+DPLCPVSVMKDLEKGMGEGSKLVIF GRGHGFVH PGSPEED+DA
Sbjct: 241 ATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDA 300

Query: 301 EHAFLMLRNWLHDGLVATN 314
           E AFLMLRNWLH GLVATN
Sbjct: 301 EQAFLMLRNWLHHGLVATN 303

BLAST of Lag0039782 vs. ExPASy TrEMBL
Match: A0A0A0KT04 (DLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G652280 PE=4 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 2.8e-113
Identity = 225/319 (70.53%), Postives = 247/319 (77.43%), Query Frame = 0

Query: 1   MELASAGAQLISRLPTPTGTGTGTGSSGIISTL-TPFLKGSVYLKSSKRERKG-----GK 60
           M  ASAGAQLIS  P      TG+ S  +      P+ K +  L S K    G     GK
Sbjct: 1   MGFASAGAQLISLPP------TGSSSRRVGKPFQPPYFKFAYPLPSRKYSNMGVWRWKGK 60

Query: 61  VCYSYSLQGGVKKDEIIIDDDDDCELVNGMELSIGEEKPEPHHTIPAYLFKPLKNNNGTA 120
           +   YS++G +K D    DDDD CELVNGMELSIGE   +PHH+IPAYLFK +KNNNGTA
Sbjct: 61  L--RYSVEGRMKDD----DDDDACELVNGMELSIGE---QPHHSIPAYLFKAVKNNNGTA 120

Query: 121 ILLLSDLFAFQHSSTTDFAYRLACNGFNVLLPDLFHGDPWKKKQPVEQSTKQDMQRIAED 180
           ILLLSDLFAFQHSS  DFAYRLAC GFNVLLPDL H D W +K P E   KQ+MQRIAED
Sbjct: 121 ILLLSDLFAFQHSSIRDFAYRLACIGFNVLLPDLVHEDVW-EKPPAEHCAKQEMQRIAED 180

Query: 181 IGRATKWLVDEFSAAGISRKLGIMGFGYGGGQVIDVLARDQGACFSIGASFYGTKVDTSL 240
           IG  TKWL +EFSAAG+S KLGI+GFGYGGG+VIDVLARD+G CF IGAS YGT+VD SL
Sbjct: 181 IGTVTKWLTEEFSAAGLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSL 240

Query: 241 ASKVKVPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDA 300
           A+KVKVPVLLISGDNDPLCPVSVMKDLEKG+GEGSKLVIFGGRGHGFVH PGSPEED+DA
Sbjct: 241 ATKVKVPVLLISGDNDPLCPVSVMKDLEKGIGEGSKLVIFGGRGHGFVHNPGSPEEDDDA 300

Query: 301 EHAFLMLRNWLHDGLVATN 314
           E AFLMLRNWLH GLVA N
Sbjct: 301 EKAFLMLRNWLHHGLVAIN 303

BLAST of Lag0039782 vs. ExPASy TrEMBL
Match: A0A6J1I908 (carboxymethylenebutenolidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111472595 PE=4 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 6.1e-108
Identity = 219/314 (69.75%), Postives = 242/314 (77.07%), Query Frame = 0

Query: 1   MELASAGAQLISRLPT-PTGTGTGTGSSGIISTLTPFLKGSVYLKSSKRERKGGKVCYSY 60
           M  ASAGAQ IS  PT  +G   G           P       LK  KRE+KGGKVCYS 
Sbjct: 1   MGSASAGAQWISAPPTGRSGRRMGIPFQPSFRCAFPISNNIGQLK-GKREKKGGKVCYSA 60

Query: 61  SLQGGVKKDEIIIDDDDDCELVNGMELSIGEEKPEPHHTIPAYLFKPLKNNNGTAILLLS 120
               GVK +   ID ++ CELV+GMELSIGE   E HH+I A+LFK +KNNNGTAILLL+
Sbjct: 61  E---GVKDE---IDAEEACELVSGMELSIGE---EAHHSITAHLFKAVKNNNGTAILLLT 120

Query: 121 DLFAFQHSSTTDFAYRLACNGFNVLLPDLFHGDPWKKKQPVEQSTKQDMQRIAEDIGRAT 180
           DLF+FQ SS  DFAYRLACNGFNVLLPD+FHGD  ++            Q+  EDIG AT
Sbjct: 121 DLFSFQDSSVRDFAYRLACNGFNVLLPDVFHGDSCEE------------QKAVEDIGAAT 180

Query: 181 KWLVDEFSAAGISRKLGIMGFGYGGGQVIDVLARDQGACFSIGASFYGTKVDTSLASKVK 240
           KWLVDEF AAGISRKLGI+GFGYGGG+VIDVLA+DQG+ FSIGAS YGT+V+ SLA+KV+
Sbjct: 181 KWLVDEFLAAGISRKLGIVGFGYGGGRVIDVLAQDQGSYFSIGASLYGTEVNPSLATKVE 240

Query: 241 VPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEHAFL 300
           VPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAE AFL
Sbjct: 241 VPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFL 292

Query: 301 MLRNWLHDGLVATN 314
           MLRNWLHDGLVATN
Sbjct: 301 MLRNWLHDGLVATN 292

BLAST of Lag0039782 vs. TAIR 10
Match: AT1G35420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 292.7 bits (748), Expect = 3.4e-79
Identity = 139/268 (51.87%), Postives = 190/268 (70.90%), Query Frame = 0

Query: 50  RKGGKVCYSYSLQGGVKKDEIIIDDDDD---CELVNGMELSIGEEKPEPHHTIPAYLFKP 109
           R+G  +   Y+L+       + +DDD++   CELVNG E+S+          +  YL   
Sbjct: 51  RRGSSLKNIYALETSDGAINVEVDDDEEEEACELVNGTEVSV--------DGVEGYLLTA 110

Query: 110 LKNNNGTAILLLSDLFAFQHSSTTDFAYRLACNGFNVLLPDLFHGDPWKKKQPVEQSTK- 169
           +KNNNGT +LLLSD+F FQ S+T DFAYR+ACNG+NVL+PDLF GDPW K +P  +  + 
Sbjct: 111 VKNNNGTGLLLLSDVFGFQDSATRDFAYRVACNGYNVLVPDLFRGDPWSKNRPKSEYEEW 170

Query: 170 ---QDMQRIAEDIGRATKWLVDEFSAAGISRKLGIMGFGYGGGQVIDVLARDQGACFSIG 229
               D  RI +D    TKW+V+EF+AAGIS+KLG+MGF +GGG+V+DVLA D+   FS G
Sbjct: 171 RRGHDPNRIRQDTTTFTKWMVEEFAAAGISKKLGVMGFCFGGGRVVDVLATDESGYFSTG 230

Query: 230 ASFYGTKVDTSLASKVKVPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFV 289
            SFYGT++D+++A  VKVPVL I+GD DPLC V  + ++E+ +GEGSK+V++ GRGHGFV
Sbjct: 231 VSFYGTRIDSAVAGDVKVPVLFIAGDRDPLCEVKGLYEIEEKIGEGSKVVVYEGRGHGFV 290

Query: 290 HKPGSPEEDEDAEHAFLMLRNWLHDGLV 311
           H+P +PE+D DAE AF ++RNWLH  L+
Sbjct: 291 HRPETPEDDRDAEEAFALMRNWLHHHLI 310

BLAST of Lag0039782 vs. TAIR 10
Match: AT1G35420.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 292.7 bits (748), Expect = 3.4e-79
Identity = 139/268 (51.87%), Postives = 190/268 (70.90%), Query Frame = 0

Query: 50  RKGGKVCYSYSLQGGVKKDEIIIDDDDD---CELVNGMELSIGEEKPEPHHTIPAYLFKP 109
           R+G  +   Y+L+       + +DDD++   CELVNG E+S+          +  YL   
Sbjct: 56  RRGSSLKNIYALETSDGAINVEVDDDEEEEACELVNGTEVSV--------DGVEGYLLTA 115

Query: 110 LKNNNGTAILLLSDLFAFQHSSTTDFAYRLACNGFNVLLPDLFHGDPWKKKQPVEQSTK- 169
           +KNNNGT +LLLSD+F FQ S+T DFAYR+ACNG+NVL+PDLF GDPW K +P  +  + 
Sbjct: 116 VKNNNGTGLLLLSDVFGFQDSATRDFAYRVACNGYNVLVPDLFRGDPWSKNRPKSEYEEW 175

Query: 170 ---QDMQRIAEDIGRATKWLVDEFSAAGISRKLGIMGFGYGGGQVIDVLARDQGACFSIG 229
               D  RI +D    TKW+V+EF+AAGIS+KLG+MGF +GGG+V+DVLA D+   FS G
Sbjct: 176 RRGHDPNRIRQDTTTFTKWMVEEFAAAGISKKLGVMGFCFGGGRVVDVLATDESGYFSTG 235

Query: 230 ASFYGTKVDTSLASKVKVPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFV 289
            SFYGT++D+++A  VKVPVL I+GD DPLC V  + ++E+ +GEGSK+V++ GRGHGFV
Sbjct: 236 VSFYGTRIDSAVAGDVKVPVLFIAGDRDPLCEVKGLYEIEEKIGEGSKVVVYEGRGHGFV 295

Query: 290 HKPGSPEEDEDAEHAFLMLRNWLHDGLV 311
           H+P +PE+D DAE AF ++RNWLH  L+
Sbjct: 296 HRPETPEDDRDAEEAFALMRNWLHHHLI 315

BLAST of Lag0039782 vs. TAIR 10
Match: AT3G23570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 51.6 bits (122), Expect = 1.3e-06
Identity = 46/202 (22.77%), Postives = 86/202 (42.57%), Query Frame = 0

Query: 114 AILLLSDLFAFQHSSTTDFAYRLACNGFNVLLPDLFHGDPWKKKQPVEQSTKQDMQRIAE 173
           A+LL+  +F ++  +    A ++A  GF  ++PD FHGDP+  +               E
Sbjct: 41  AVLLVPHVFGYETPNLRKLADKVAEAGFYAVVPDFFHGDPYNPENQDRPFPIWMKDHELE 100

Query: 174 DIGRATKWLVDEFSAAGISRKLGIMGFGYGGGQVIDVLARDQGACFSIGASFYGTKVDTS 233
                +K +V+     GI+  +G  GF +G    ++ LA+++          +  +V   
Sbjct: 101 KGFEESKPIVEALKNKGIT-SIGAAGFCWGAKVAVE-LAKEK--LVDATVLLHPARVTVD 160

Query: 234 LASKVKVPVLLISGDNDPLCPVSVMKDLEKGMGE----GSKLVIFGGRGHGFV--HKPGS 293
              +V +P+ ++  + D + P  +++  E  +       S + IF    HG+   +    
Sbjct: 161 DIKEVNLPIAVLGAEIDQVSPPELVRQFEDILASKPQVKSFVKIFPRCKHGWTVRYNEND 220

Query: 294 PEEDEDAEHAFLMLRNWLHDGL 310
           P E E A  A   +  WL D L
Sbjct: 221 PSEVEAAMEAHKDMLAWLIDYL 238

BLAST of Lag0039782 vs. TAIR 10
Match: AT3G23600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 51.2 bits (121), Expect = 1.7e-06
Identity = 43/196 (21.94%), Postives = 86/196 (43.88%), Query Frame = 0

Query: 115 ILLLSDLFAFQHSSTTDFAYRLACNGFNVLLPDLFHGDPWKKKQPVEQSTKQDMQRIAED 174
           +LL+SD+F F+  +    A ++A +GF V++PD F GDP+                  + 
Sbjct: 42  VLLISDIFGFEAPNLRALADKVAASGFYVVVPDYFGGDPYNPSNQDRPIPVWIKDHGCDK 101

Query: 175 IGRATKWLVDEFSAAGISRKLGIMGFGYGGGQVIDVLARDQGACFSIGASFYGTKVDTSL 234
               TK +++     GI+  +G  G  +G   V++ L++++          + + V+   
Sbjct: 102 GFENTKPVLETIKNKGIT-AIGAAGMCWGAKVVVE-LSKEE--LIQAAVLLHPSFVNVDD 161

Query: 235 ASKVKVPVLLISGDNDPLCPVSVMKDLEKGMGE----GSKLVIFGGRGHGFV--HKPGSP 294
               K P+ ++  + D + P +++K  E+ +       S + I     HG+   +    P
Sbjct: 162 IKGGKAPIAILGAEIDQMSPPALLKQFEEILSSKPEVNSYVKIHPKVSHGWTVRYNIDEP 221

Query: 295 EEDEDAEHAFLMLRNW 305
           E  + AE A   + +W
Sbjct: 222 EAVKAAEEAHKEMLDW 233

BLAST of Lag0039782 vs. TAIR 10
Match: AT3G23600.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 48.1 bits (113), Expect = 1.5e-05
Identity = 42/196 (21.43%), Postives = 81/196 (41.33%), Query Frame = 0

Query: 115 ILLLSDLFAFQHSSTTDFAYRLACNGFNVLLPDLFHGDPWKKKQPVEQSTKQDMQRIAED 174
           +LL+SD+F F+  +    A ++A +GF V++PD F GDP+                  + 
Sbjct: 42  VLLISDIFGFEAPNLRALADKVAASGFYVVVPDYFGGDPYNPSNQDRPIPVWIKDHGCDK 101

Query: 175 IGRATKWLVDEFSAAGISRKLGIMGFGYGGGQVIDVLARDQGACFSIGASFYGTKVDTSL 234
               TK +++     GI+  +G  G  +G      V+   +          + + V+   
Sbjct: 102 GFENTKPVLETIKNKGIT-AIGAAGMCWG------VVELSKEELIQAAVLLHPSFVNVDD 161

Query: 235 ASKVKVPVLLISGDNDPLCPVSVMKDLEKGMGE----GSKLVIFGGRGHGFV--HKPGSP 294
               K P+ ++  + D + P +++K  E+ +       S + I     HG+   +    P
Sbjct: 162 IKGGKAPIAILGAEIDQMSPPALLKQFEEILSSKPEVNSYVKIHPKVSHGWTVRYNIDEP 221

Query: 295 EEDEDAEHAFLMLRNW 305
           E  + AE A   + +W
Sbjct: 222 EAVKAAEEAHKEMLDW 230