Lag0038030 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0038030
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Cytochrome P450
Location	chr2: 11754999 .. 11757906 (-)
RNA-Seq Expression	Lag0038030
Synteny	Lag0038030

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATCGTCTTCTTCACAATCTTCTCTCTTCTCATCTCCATCTTCCTCTTCCTTCTTACAGGAAGAAGATCATCCCAAAAGCTTCCTCCAGGTTCTCTAGGGTTTCCCTTCATCGGCCAAAGCCTCGGTCTCCTTCGCGCCATGCGGACCAACACTGCAGAGGAATGGCTGCAGCAGAGGATCAGAAAGTACGGCCCGATTTCGAAGCTAACACTCTTTGGGAAGCCCACGGTGTTCGTCTGCGGGCAGGGCGCCAACAAGTTGATCTTCAATGGCGATTCCGCAGTTTCGAACCAGCAGAATGAGTCGCTTAGGGCAATTTTGGGGGAAAGTAATTTGTTGGAGTTGAGTGGGGATGATCACAAGCGTGTCAGAGGTGCGCTTGTTTCGTTCTTGAAGCCGGAGTGTTTGAAGCAGTATGTGGGGAAAATGGATGAGGAAATCAGGCGACATATCGAGATGCATTGGCAGGGGAAGCAAGAGGTCACGGTGAGTGGATATCCCAACAAAATTTTTCCATGTTTTTCATTATTAATAGTGGTTGATTTTAACTTGAATCTGTGGTCTATGTGAGATTTTTTTTTTTTTTGTATTAACTGGCTGTTGGGCTGAGGCAGATGATCCTCAGTTGATGATGGCGACTTGGTCTGTTGGTGCTCAATAAATTGGATTAACGTTGAAATCTACTCTGAAAATATTCTAGTTGAAGTAACTTGGTTTGATCAAGTTACAGTTAATTTAGTGGGGAATGGTTGTTTGACAAGAATTGATCCGCACAGGTTTTTGTATGGCTTGAAACTTAAACTAATAAAAGAAAGATTAAATTACAAATTAGGCCTGTGAACTTTTAAAGTTGTGTCTAATAGGTTCTTAAACTTAAAATGTGTTAGGCCCCTAAACTTTCAATGATGTATAATACAATCTTGAACTATTCATTTTGCATATAATAAATCCTTCCACTTTAAGAAATCTCTCATAAATTAGAGATATTTTAGATACAAACTTCAAAATTTAAGTATCTATCGAACATTTTTTTTAAAAAAAAAACTATCGAACATTTTGTAAAGTTGGAGAACTTATTAGACATAACATAACAGTGAAAGTTCAGAGATTTATTAGCCACTTTATAAAATCTGGGGATTTATCAAATACCTTTGGAAGTTTACGGACCAAACCAGTAATTTAACCTAAAATAAAAATATACCATCGCCTTTGGCTCTTTAATTTTCCTAGGCAGCGGGTGTTGGTGGAAGAAATCTTAGAGAAGATGGTTGTCTAATGGCTTCTAAGAGGTGTCCTCTAATTACTAGGACGAGGCTGGTATCCTTTTACTTTGGCAGACTAAGCTATAGATATGTAGGGAAAAAAAAAAAAAAGAAAAACAAAACTTGGTTCTTTCTTTCGTATCAAATCCTGAGAAGATCCTTCATGATTTGATGACATTGGTTATATCTGAATTATGCTTGAATTTGACTCATAGAATCTTTTGTCAATTGACTTGCTGTTTCATATGCCAATAGGTACTGCCATTGATGAAGACTCTCACCTTCAACATTGTATGCTCTCTTCTCTTTGGTCTTGAGCAAGGTACGAGAAGAGAAAAAATGATAGAATGCTTTCGAGTAATGATAGGAGGGGTATGGTCGATCCCAATAAACTTGCCATTCACGAGGTACAACCAAAGTCGACGGGCAAGTAAGAGAATCCAAGAAATGCTTAAGAACCTCTTGGATGAAAAGAGAGTTGAATTGGAAGAAAAGGGTGCTCCTCCACACCAAGATCTAATTACTTGTTTGCTAAGCATCAGAAATGAAGAAAATGAACTACTGTTGACTGAGAAGGAAATTGTGCACAATATTTTACTGGTAATGGTTGCTGGGTTCGATACTTCATCTGTTCTGATTACATTCATGATGCGGATTTTGGCTAATAATCCAACTGTTTATGCAGCTGTTCTTCAAGGTATACTTGCAATTATCTCTCTAACTGTCAAGATTACCATGAGCTTATAAGCATCTGAGTCCTAAAATGTAGAGATTATAATTTTATTTTTAGTAGAAACCCAGAGAAACTCCTGTTTTAGAATGAAATACAGAAGTACTCAGATTAAGAAATTGGTATGGCAAAATGAAAAATACAAACTCGATAACTTTTACCCTAAAAGGAAAAACAGTATAAACAAAATCAGAAATTGGTAAAGGAACTCTAGATAGTAAGAAACTTACCCATCGACAGATTAAAAATGGGAAGGACTTCTAACTAGAAGCATACAAACTTGAGGTGGAAAAAGTTTTATGATGTCAGGTTTCTTGTGGTCTGTGGTTACAGAACAAGAAGAAATAGCTAGAGGCAAGGGATGTGGAGAGTTGCTCACTTGGGAAGATCTTGCCAAGATGAAATACACATGGAGAGTTGCATTAGAGACCCTAAGGTTAGTGGCACCAATATTTGGAGGGTTCAGAAGGGCTATGAAAGATATTGAATTTGGTGGCTACCTTATTCCTAAAGGATGGCAAGTAAGTAAAGTTTTCTTCTTTTTTATTTAGCTTGAAAAAACACATGTGAAAATATTCATCCAACAACAATTGGATTTCAGATATTCTGGGCATCACCAATGACTCATTTGGATGATACTATATTTAAAGAACCATTGAAGTTCGACCCGAATAGATTCGAGAATCAAGCATCGGTTCCACCGTATTGCTTCATTGGATTTGGAGGAGGTCCAAGAATTTGTCCAGGCAATGAGTTTGCAAGGGTTGAAACTCTTGTTACGATCCATTACTTGATCACCCAATTCACATGGAAGCTACTTTCAGATGACCATTTTATTAGAGATCCAATGCCAGTACCTACCAAAGGATTGCCTATCAGAATTTTTCCTAGAGAAACTTCCATGTCATTGTAG

mRNA sequence

ATGATCGTCTTCTTCACAATCTTCTCTCTTCTCATCTCCATCTTCCTCTTCCTTCTTACAGGAAGAAGATCATCCCAAAAGCTTCCTCCAGGTTCTCTAGGGTTTCCCTTCATCGGCCAAAGCCTCGGTCTCCTTCGCGCCATGCGGACCAACACTGCAGAGGAATGGCTGCAGCAGAGGATCAGAAAGTACGGCCCGATTTCGAAGCTAACACTCTTTGGGAAGCCCACGGTGTTCGTCTGCGGGCAGGGCGCCAACAAGTTGATCTTCAATGGCGATTCCGCAGTTTCGAACCAGCAGAATGAGTCGCTTAGGGCAATTTTGGGGGAAAGTAATTTGTTGGAGTTGAGTGGGGATGATCACAAGCGTGTCAGAGGTGCGCTTGTTTCGTTCTTGAAGCCGGAGTGTTTGAAGCAGTATGTGGGGAAAATGGATGAGGAAATCAGGCGACATATCGAGATGCATTGGCAGGGGAAGCAAGAGGTCACGGTACTGCCATTGATGAAGACTCTCACCTTCAACATTGTATGCTCTCTTCTCTTTGGTCTTGAGCAAGGTACGAGAAGAGAAAAAATGATAGAATGCTTTCGAGTAATGATAGGAGGGGTATGGTCGATCCCAATAAACTTGCCATTCACGAGGTACAACCAAAGTCGACGGGCAAGTAAGAGAATCCAAGAAATGCTTAAGAACCTCTTGGATGAAAAGAGAGTTGAATTGGAAGAAAAGGGTGCTCCTCCACACCAAGATCTAATTACTTGTTTGCTAAGCATCAGAAATGAAGAAAATGAACTACTGTTGACTGAGAAGGAAATTGTGCACAATATTTTACTGGTAATGGTTGCTGGGTTCGATACTTCATCTGTTCTGATTACATTCATGATGCGGATTTTGGCTAATAATCCAACTGTTTATGCAGCTGTTCTTCAAGAACAAGAAGAAATAGCTAGAGGCAAGGGATGTGGAGAGTTGCTCACTTGGGAAGATCTTGCCAAGATGAAATACACATGGAGAGTTGCATTAGAGACCCTAAGGTTAGTGGCACCAATATTTGGAGGGTTCAGAAGGGCTATGAAAGATATTGAATTTGGTGGCTACCTTATTCCTAAAGGATGGCAAATATTCTGGGCATCACCAATGACTCATTTGGATGATACTATATTTAAAGAACCATTGAAGTTCGACCCGAATAGATTCGAGAATCAAGCATCGGTTCCACCGTATTGCTTCATTGGATTTGGAGGAGGTCCAAGAATTTGTCCAGGCAATGAGTTTGCAAGGGTTGAAACTCTTGTTACGATCCATTACTTGATCACCCAATTCACATGGAAGCTACTTTCAGATGACCATTTTATTAGAGATCCAATGCCAGTACCTACCAAAGGATTGCCTATCAGAATTTTTCCTAGAGAAACTTCCATGTCATTGTAG

Coding sequence (CDS)

ATGATCGTCTTCTTCACAATCTTCTCTCTTCTCATCTCCATCTTCCTCTTCCTTCTTACAGGAAGAAGATCATCCCAAAAGCTTCCTCCAGGTTCTCTAGGGTTTCCCTTCATCGGCCAAAGCCTCGGTCTCCTTCGCGCCATGCGGACCAACACTGCAGAGGAATGGCTGCAGCAGAGGATCAGAAAGTACGGCCCGATTTCGAAGCTAACACTCTTTGGGAAGCCCACGGTGTTCGTCTGCGGGCAGGGCGCCAACAAGTTGATCTTCAATGGCGATTCCGCAGTTTCGAACCAGCAGAATGAGTCGCTTAGGGCAATTTTGGGGGAAAGTAATTTGTTGGAGTTGAGTGGGGATGATCACAAGCGTGTCAGAGGTGCGCTTGTTTCGTTCTTGAAGCCGGAGTGTTTGAAGCAGTATGTGGGGAAAATGGATGAGGAAATCAGGCGACATATCGAGATGCATTGGCAGGGGAAGCAAGAGGTCACGGTACTGCCATTGATGAAGACTCTCACCTTCAACATTGTATGCTCTCTTCTCTTTGGTCTTGAGCAAGGTACGAGAAGAGAAAAAATGATAGAATGCTTTCGAGTAATGATAGGAGGGGTATGGTCGATCCCAATAAACTTGCCATTCACGAGGTACAACCAAAGTCGACGGGCAAGTAAGAGAATCCAAGAAATGCTTAAGAACCTCTTGGATGAAAAGAGAGTTGAATTGGAAGAAAAGGGTGCTCCTCCACACCAAGATCTAATTACTTGTTTGCTAAGCATCAGAAATGAAGAAAATGAACTACTGTTGACTGAGAAGGAAATTGTGCACAATATTTTACTGGTAATGGTTGCTGGGTTCGATACTTCATCTGTTCTGATTACATTCATGATGCGGATTTTGGCTAATAATCCAACTGTTTATGCAGCTGTTCTTCAAGAACAAGAAGAAATAGCTAGAGGCAAGGGATGTGGAGAGTTGCTCACTTGGGAAGATCTTGCCAAGATGAAATACACATGGAGAGTTGCATTAGAGACCCTAAGGTTAGTGGCACCAATATTTGGAGGGTTCAGAAGGGCTATGAAAGATATTGAATTTGGTGGCTACCTTATTCCTAAAGGATGGCAAATATTCTGGGCATCACCAATGACTCATTTGGATGATACTATATTTAAAGAACCATTGAAGTTCGACCCGAATAGATTCGAGAATCAAGCATCGGTTCCACCGTATTGCTTCATTGGATTTGGAGGAGGTCCAAGAATTTGTCCAGGCAATGAGTTTGCAAGGGTTGAAACTCTTGTTACGATCCATTACTTGATCACCCAATTCACATGGAAGCTACTTTCAGATGACCATTTTATTAGAGATCCAATGCCAGTACCTACCAAAGGATTGCCTATCAGAATTTTTCCTAGAGAAACTTCCATGTCATTGTAG

Protein sequence

MIVFFTIFSLLISIFLFLLTGRRSSQKLPPGSLGFPFIGQSLGLLRAMRTNTAEEWLQQRIRKYGPISKLTLFGKPTVFVCGQGANKLIFNGDSAVSNQQNESLRAILGESNLLELSGDDHKRVRGALVSFLKPECLKQYVGKMDEEIRRHIEMHWQGKQEVTVLPLMKTLTFNIVCSLLFGLEQGTRREKMIECFRVMIGGVWSIPINLPFTRYNQSRRASKRIQEMLKNLLDEKRVELEEKGAPPHQDLITCLLSIRNEENELLLTEKEIVHNILLVMVAGFDTSSVLITFMMRILANNPTVYAAVLQEQEEIARGKGCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRAMKDIEFGGYLIPKGWQIFWASPMTHLDDTIFKEPLKFDPNRFENQASVPPYCFIGFGGGPRICPGNEFARVETLVTIHYLITQFTWKLLSDDHFIRDPMPVPTKGLPIRIFPRETSMSL

Homology

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BLAST of Lag0038030 vs. NCBI nr
Match: XP_022139355.1 (LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Momordica charantia])

HSP 1 Score: 857.8 bits (2215), Expect = 4.3e-245
Identity = 419/478 (87.66%), Postives = 449/478 (93.93%), Query Frame = 0


Query: 1   MIVFFTIFSLLISIFLFLLTGR--RSSQKLPPGSLGFPFIGQSLGLLRAMRTNTAEEWLQ 60
           M ++ TIFS LI IFLFL  GR  RSSQKLPPGSLG PFIGQSLGLLRAMR+NTAE+WLQ
Sbjct: 1   MNIYLTIFSFLIPIFLFLTRGRRSRSSQKLPPGSLGVPFIGQSLGLLRAMRSNTAEQWLQ 60

Query: 61  QRIRKYGPISKLTLFGKPTVFVCGQGANKLIFNGDSAVSNQQNESLRAILGESNLLELSG 120
           +RI KYGPISKLTLFGKPTV V GQGANKLIFNGD+A+SNQQNESLRAILGESNLLEL+G
Sbjct: 61  RRITKYGPISKLTLFGKPTVLVGGQGANKLIFNGDTAISNQQNESLRAILGESNLLELTG 120

Query: 121 DDHKRVRGALVSFLKPECLKQYVGKMDEEIRRHIEMHWQGKQEVTVLPLMKTLTFNIVCS 180
           DDHKRVR ALV FLKPECLKQYVGKMDEE+RRH+EMHWQ KQEVTVLPLMKTLTFNIVCS
Sbjct: 121 DDHKRVRSALVXFLKPECLKQYVGKMDEEVRRHLEMHWQXKQEVTVLPLMKTLTFNIVCS 180

Query: 181 LLFGLEQGTRREKMIECFRVMIGGVWSIPINLPFTRYNQSRRASKRIQEMLKNLLDEKRV 240
           LLFGLE+GTRRE+MIECF+VMIGGVWSIPINLPFTRYNQSR+AS +IQEMLKNLLDEKRV
Sbjct: 181 LLFGLEEGTRRERMIECFQVMIGGVWSIPINLPFTRYNQSRQASIKIQEMLKNLLDEKRV 240

Query: 241 ELEEKGAPPHQDLITCLLSIRNEENELLLTEKEIVHNILLVMVAGFDTSSVLITFMMRIL 300
           ELE KG  PHQDLITCLLSI+N+E+E +LTEKEI+HNI+LVMVAG+DTSSVLITFM+R L
Sbjct: 241 ELEVKGTSPHQDLITCLLSIKNKEHEQVLTEKEIIHNIILVMVAGYDTSSVLITFMVRFL 300

Query: 301 ANNPTVYAAVLQEQEEIARGKGCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRAM 360
           ANNPTVYAAVLQEQEEIAR K CGELLTWEDLAKMKYTWRVALETLRLVAP+FGGFR+AM
Sbjct: 301 ANNPTVYAAVLQEQEEIARSKECGELLTWEDLAKMKYTWRVALETLRLVAPVFGGFRKAM 360

Query: 361 KDIEFGGYLIPKGWQIFWASPMTHLDDTIFKEPLKFDPNRFENQASVPPYCFIGFGGGPR 420
           KDIEFGGYLIPKGWQIFWASPMTHLDDTIF+EPLKFDPNRFENQASVP YCF+ FGGGPR
Sbjct: 361 KDIEFGGYLIPKGWQIFWASPMTHLDDTIFEEPLKFDPNRFENQASVPXYCFVAFGGGPR 420

Query: 421 ICPGNEFARVETLVTIHYLITQFTWKLLSDDHFIRDPMPVPTKGLPIRIFPRETSMSL 477
           ICPG EFARVETLVTIHYLITQFTWKLL DD FIRDPMPVPTKGLPI++ PRETSM+L
Sbjct: 421 ICPGYEFARVETLVTIHYLITQFTWKLLLDDLFIRDPMPVPTKGLPIKVCPRETSMTL 478

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BLAST of Lag0038030 vs. NCBI nr
Match: XP_038899130.1 (LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Benincasa hispida])

HSP 1 Score: 834.7 bits (2155), Expect = 3.9e-238
Identity = 411/483 (85.09%), Postives = 439/483 (90.89%), Query Frame = 0


Query: 1   MIVFFTIFSLLISIFLFLLTGRRSSQKLPPGSLGFPFIGQSLGLLRAMRTNTAEEWLQQR 60
           +I   T FSLLI IFLFLLTGR+SS+ LPPGSLGFPFIGQSL LLRAMR NTAEEW QQR
Sbjct: 2   IIYLITTFSLLIPIFLFLLTGRKSSKNLPPGSLGFPFIGQSLSLLRAMRINTAEEWAQQR 61

Query: 61  IRKYGPISKLTLFGKPTVFVCGQGANKLIFNGDSAVSNQQNESLRAILGESNLLELSGDD 120
           +RKYG ISKLTLFGKPTVFV GQ  +KLIF+GD  +SNQQNESLRAILGE NLLEL G+D
Sbjct: 62  MRKYGAISKLTLFGKPTVFVGGQSGHKLIFSGDCGISNQQNESLRAILGECNLLELIGED 121

Query: 121 HKRVRGALVSFLKPECLKQYVGKMDEEIRRHIEMHWQGKQEVTVLPLMKTLTFNIVCSLL 180
           HKRVR ALVSFLKPECLKQYVGKMD  IR+HI+MHWQGKQ+VTVLPLMKTLTFNIVCSLL
Sbjct: 122 HKRVRNALVSFLKPECLKQYVGKMD-XIRKHIKMHWQGKQQVTVLPLMKTLTFNIVCSLL 181

Query: 181 FGLEQGTRREKMIECFRVMIGGVWSIPINLPFTRYNQSRRASKRIQEMLKNLLDEKRVEL 240
           FGLEQG RRE+MIECFRVMIGGVWSIPINLPFTRYNQSRRAS+RIQEM+K LLDEKRVEL
Sbjct: 182 FGLEQGMRRERMIECFRVMIGGVWSIPINLPFTRYNQSRRASRRIQEMIKELLDEKRVEL 241

Query: 241 EEKGAPPHQDLITCLLSIRNEENELLLTEKEIVHNILLVMVAGFDTSSVLITFMMRILAN 300
           EEK +  HQDLITCLLSI NEENEL+LTEKEIVHNI+LVMVAGFDTSSVLITF+MR LAN
Sbjct: 242 EEKRSSSHQDLITCLLSIGNEENELILTEKEIVHNIMLVMVAGFDTSSVLITFIMRFLAN 301

Query: 301 NPTVYAAVLQEQEEIARGKGCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRAMKD 360
           NPTVY AVLQEQEEIAR K CGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFR+AM D
Sbjct: 302 NPTVYVAVLQEQEEIARSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMND 361

Query: 361 IEFGGYLIPKGWQIFWASPMTHLDDTIFKEPLKFDPNRFENQASVPPYCFIGFG------ 420
           IEFGGYLIPKGWQIFWASP+THLD TIF+EPLKFDPNRF+N  S+PPYCF+GFG      
Sbjct: 362 IEFGGYLIPKGWQIFWASPVTHLDGTIFREPLKFDPNRFDNPTSIPPYCFVGFGGGPRIC 421

Query: 421 -GGPRICPGNEFARVETLVTIHYLITQFTWKLLSDDHFIRDPMPVPTKGLPIRIFPRETS 477
            GGPRICPGNEFARVETLVTIHYLIT+FTWKLL DDHFIRDPMP PTKG+PI+I PRETS
Sbjct: 422 PGGPRICPGNEFARVETLVTIHYLITRFTWKLLLDDHFIRDPMPTPTKGMPIKICPRETS 481

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BLAST of Lag0038030 vs. NCBI nr
Match: XP_008457111.1 (PREDICTED: cytochrome P450 716B1-like [Cucumis melo] >KAA0047356.1 cytochrome P450 716B1-like protein [Cucumis melo var. makuwa] >TYK14034.1 cytochrome P450 716B1-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 823.2 bits (2125), Expect = 1.2e-234
Identity = 405/477 (84.91%), Postives = 434/477 (90.99%), Query Frame = 0


Query: 1   MIVFFTI-FSLLISIFLFLLTGRRSSQKLPPGSLGFPFIGQSLGLLRAMRTNTAEEWLQQ 60
           MI++ TI F LLI IFLFL T  +  Q LPPGSLGFPFIGQSL LLRAMRTNTAE+W QQ
Sbjct: 1   MIIYLTILFCLLIPIFLFLRTSSK-KQHLPPGSLGFPFIGQSLSLLRAMRTNTAEQWAQQ 60

Query: 61  RIRKYGPISKLTLFGKPTVFVCGQGANKLIFNGDSA-VSNQQNESLRAILGESNLLELSG 120
           RIR YG ISKLTLFGKPTVF+ GQ  NKLIF+GD A VSNQQNESLRAILGE NLLEL+G
Sbjct: 61  RIRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELTG 120

Query: 121 DDHKRVRGALVSFLKPECLKQYVGKMDEEIRRHIEMHWQGKQEVTVLPLMKTLTFNIVCS 180
           +DHKRVR ALVSFLKPECLK+YVGKMDEEIR HI MHW  KQEVTVLPLMKTLTFNIVCS
Sbjct: 121 EDHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIHMHWHRKQEVTVLPLMKTLTFNIVCS 180

Query: 181 LLFGLEQGTRREKMIECFRVMIGGVWSIPINLPFTRYNQSRRASKRIQEMLKNLLDEKRV 240
           LLFGLEQG RRE+MIECFRVMI GVWSIPINLPFTRYNQSRRAS++IQEMLK LLDEKRV
Sbjct: 181 LLFGLEQGMRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRV 240

Query: 241 ELEEKGAPPHQDLITCLLSIRNEENELLLTEKEIVHNILLVMVAGFDTSSVLITFMMRIL 300
           ELEEKG   HQDLITCLLSIRNEENEL+L+EKEIVHNI+LVMVAGFDTSSVLITFMMR L
Sbjct: 241 ELEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRSL 300

Query: 301 ANNPTVYAAVLQEQEEIARGKGCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRAM 360
           ANNPTVYAAVLQEQEEIAR K CGELL WEDLAKMKYTWRVALETLRLVAPIFGGFR+AM
Sbjct: 301 ANNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAM 360

Query: 361 KDIEFGGYLIPKGWQIFWASPMTHLDDTIFKEPLKFDPNRFENQASVPPYCFIGFGGGPR 420
            DI+FGGYLIPKGWQIFW SP+THLDD IF+EP KFDP+RF+N AS+PPYCF+GFG GPR
Sbjct: 361 NDIQFGGYLIPKGWQIFWTSPVTHLDDNIFREPSKFDPDRFKNPASIPPYCFLGFGSGPR 420

Query: 421 ICPGNEFARVETLVTIHYLITQFTWKLLSDDHFIRDPMPVPTKGLPIRIFPRETSMS 476
           ICPGNEFARVETLVTIHYLITQFTWKL+SDDHFIRDPMP PTKG+PI+I+PR+T M+
Sbjct: 421 ICPGNEFARVETLVTIHYLITQFTWKLISDDHFIRDPMPTPTKGMPIKIWPRQTFMA 476

[Close]

BLAST of Lag0038030 vs. NCBI nr
Match: XP_004140824.1 (cytochrome P450 716B1 [Cucumis sativus] >KGN57442.1 hypothetical protein Csa_010272 [Cucumis sativus])

HSP 1 Score: 817.8 bits (2111), Expect = 5.0e-233
Identity = 407/477 (85.32%), Postives = 433/477 (90.78%), Query Frame = 0


Query: 1   MIVFFTI-FSLLISIFLFLLTGRRSSQK--LPPGSLGFPFIGQSLGLLRAMRTNTAEEWL 60
           MI++ TI F LLI IFLFL   RRSS+K  LPPGSLGFP IGQSL LLRAMRTNTAE+W 
Sbjct: 1   MIIYLTIFFCLLIPIFLFL---RRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWA 60

Query: 61  QQRIRKYGPISKLTLFGKPTVFVCGQGANKLIFNGDSA-VSNQQNESLRAILGESNLLEL 120
           QQRIR YG ISKLTLFGKPTVF+ GQ  NKLIF+GD A VSNQQNESLRAILGE NLLEL
Sbjct: 61  QQRIRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLEL 120

Query: 121 SGDDHKRVRGALVSFLKPECLKQYVGKMDEEIRRHIEMHWQGKQEVTVLPLMKTLTFNIV 180
            G+DHKRVR ALVSFLKPECLK+YVGKMDEEIR HI MHW  KQEVTVLPLMKTLTFNIV
Sbjct: 121 IGEDHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIV 180

Query: 181 CSLLFGLEQGTRREKMIECFRVMIGGVWSIPINLPFTRYNQSRRASKRIQEMLKNLLDEK 240
           CSLLFGLEQGTRRE+MIECFRVMI GVWSIPINLPFTRYNQSRRAS++IQEMLK LLDEK
Sbjct: 181 CSLLFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEK 240

Query: 241 RVELEEKGAPPHQDLITCLLSIRNEENELLLTEKEIVHNILLVMVAGFDTSSVLITFMMR 300
           RVELEEKG   HQDLITCLLSIRNEENEL+L+EKEIVHNI+LV+VAGFDTSSVLITFMMR
Sbjct: 241 RVELEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMR 300

Query: 301 ILANNPTVYAAVLQEQEEIARGKGCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRR 360
            LANNPTVYAAVLQEQEEIAR K CGELL WEDLAKMKYTWRVALETLRLVAPIFGGFR+
Sbjct: 301 NLANNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRK 360

Query: 361 AMKDIEFGGYLIPKGWQIFWASPMTHLDDTIFKEPLKFDPNRFENQASVPPYCFIGFGGG 420
           AM DI+FGGYLIPKGWQIFW SP+THLDDTIF+EP KFDP+RF+N AS+PPYCF+GFG G
Sbjct: 361 AMNDIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSG 420

Query: 421 PRICPGNEFARVETLVTIHYLITQFTWKLLSDDHFIRDPMPVPTKGLPIRIFPRETS 474
           PRICPGNEFARVETLVTIHYLITQFTWKLL DDHFIRDPMP PTKG+PI+I+PR  S
Sbjct: 421 PRICPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQS 474

[Close]

BLAST of Lag0038030 vs. NCBI nr
Match: KAA8523694.1 (hypothetical protein F0562_010117 [Nyssa sinensis])

HSP 1 Score: 735.7 bits (1898), Expect = 2.5e-208
Identity = 346/473 (73.15%), Postives = 416/473 (87.95%), Query Frame = 0


Query: 1   MIVFFTIFSLLISIFLFLLTGRRSSQKLPPGSLGFPFIGQSLGLLRAMRTNTAEEWLQQR 60
           M +FFTIF  L+ IF  L+  R SSQ+LPPGSLG P IGQSL LL+AM+ NTAE WL++R
Sbjct: 15  MNIFFTIFLFLLPIFFLLVRRRSSSQRLPPGSLGIPIIGQSLSLLQAMKANTAERWLEER 74

Query: 61  IRKYGPISKLTLFGKPTVFVCGQGANKLIFNGD-SAVSNQQNESLRAILGESNLLELSGD 120
           IRKYG ISKL+LFGKPTVF+ GQ ANKL+F GD S ++NQQ +S+R ILG+ NLLELSG+
Sbjct: 75  IRKYGSISKLSLFGKPTVFIYGQAANKLVFTGDCSVITNQQAQSVRMILGDRNLLELSGE 134

Query: 121 DHKRVRGALVSFLKPECLKQYVGKMDEEIRRHIEMHWQGKQEVTVLPLMKTLTFNIVCSL 180
           DHKRVR ALVSFLKPECLKQYVGKM+EEIR H+EMHW+GKQ+VTVLPLMKTLTFNI+CSL
Sbjct: 135 DHKRVRDALVSFLKPECLKQYVGKMEEEIREHLEMHWEGKQKVTVLPLMKTLTFNIICSL 194

Query: 181 LFGLEQGTRREKMIECFRVMIGGVWSIPINLPFTRYNQSRRASKRIQEMLKNLLDEKRVE 240
           LFG+++G RR+K++E F+ MI G+WS+P+NLPFTRYN+S RA+ R+Q MLK+L+ EKRVE
Sbjct: 195 LFGVQRGARRDKLVEYFQEMIQGMWSVPVNLPFTRYNRSLRATARVQNMLKDLIREKRVE 254

Query: 241 LEEKGAPPHQDLITCLLSIRNEENELLLTEKEIVHNILLVMVAGFDTSSVLITFMMRILA 300
           LE++GA  HQDLITCLLSIR ++NE L++EKEIVHN++LVMVAG DTSSVLI+F+MR+L+
Sbjct: 255 LEQQGASSHQDLITCLLSIRGKDNEELISEKEIVHNVMLVMVAGHDTSSVLISFIMRLLS 314

Query: 301 NNPTVYAAVLQEQEEIARGKGCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRAMK 360
           N+PTVYAAVLQEQEEIA+ K CGELLTWEDLAKMKYTWR A+ETLR+  PIFGGFR+ +K
Sbjct: 315 NDPTVYAAVLQEQEEIAKSKPCGELLTWEDLAKMKYTWRAAMETLRMFPPIFGGFRKTLK 374

Query: 361 DIEFGGYLIPKGWQIFWASPMTHLDDTIFKEPLKFDPNRFENQASVPPYCFIGFGGGPRI 420
           DIE+ GYLIPKGWQIFWAS MTH+D +IF++P KFDP RFENQASVPPYCF+ FGGGPRI
Sbjct: 375 DIEYKGYLIPKGWQIFWASSMTHMDSSIFQDPSKFDPTRFENQASVPPYCFVAFGGGPRI 434

Query: 421 CPGNEFARVETLVTIHYLITQFTWKL-LSDDHFIRDPMPVPTKGLPIRIFPRE 472
           CPG EFAR+ETLVT+HYL+T+FTWKL  +D+ F RDPMPVPT+GLPI I P++
Sbjct: 435 CPGYEFARIETLVTVHYLVTRFTWKLCCTDNFFSRDPMPVPTEGLPIEIVPKK 487

[Close]

BLAST of Lag0038030 vs. ExPASy Swiss-Prot
Match: Q50EK1 (Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 1.2e-107
Identity = 195/475 (41.05%), Postives = 294/475 (61.89%), Query Frame = 0


Query: 3   VFFTIF---SLLISIFLFLLTGRRSS--QKLPPGSLGFPFIGQSLGLLRAMRTNTAEEWL 62
           + FT+F    + ++ F +L++  R+   + +PPG+ G+P IG++L  L   R     ++ 
Sbjct: 21  LMFTVFLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNPRDFF 80

Query: 63  QQRIRKYGPISKLTLFGKPTVFVCGQGANKLIFNGDSAVSNQQNESLRAILGESNLLELS 122
             R +KYG +   +L G PTV  C    N+ +F+ ++ +      S    L  S+L+   
Sbjct: 81  DSRTQKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENKLVVNSWPSSVGNLFRSSLITTV 140

Query: 123 GDDHKRVRGALVSFLKPECLKQYVGKMDEEIRRHIEMHWQGKQEVTVLPLMKTLTFNIVC 182
           GDD KR+R  L++FL+PE L+++VG++D   +RH+  HW GK EV  LPL+K  TF++ C
Sbjct: 141 GDDAKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVMALPLLKRYTFSLAC 200

Query: 183 SLLFGLEQGTRREKMIECFRVMIGGVWSIPINLPFTRYNQSRRASKRIQEMLKNLLDEKR 242
            L   +      +++   F V + GV  IPI+LP TRYN+++ A+  I++ L ++++E++
Sbjct: 201 DLFASINTKDDLDRLWLHFMVFVKGVMQIPIDLPGTRYNKTKHAANAIRQQLGSIINERK 260

Query: 243 VELEEKGAPPHQDLITCLLSIRNEENELLLTEKEIVHNILLVMVAGFDTSSVLITFMMRI 302
           + LE   A P QDL++ LLS  +E+ E  LT+ EI  NILL++ AG DTSS  +T +++ 
Sbjct: 261 IALEAGNASPEQDLLSFLLSNVDEQGE-SLTDNEIQDNILLLLYAGHDTSSSTLTVLLKF 320

Query: 303 LANNPTVYAAVLQEQEEIARGKGCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRA 362
           LA NP  Y  VL+EQ  IA  K  G+LL WEDL +MKY+WRVA E LRL   + G FR+A
Sbjct: 321 LAENPHCYEEVLREQLNIAGSKEEGQLLEWEDLQRMKYSWRVAQEALRLFPAVQGSFRKA 380

Query: 363 MKDIEFGGYLIPKGWQIFWASPMTHLDDTIFKEPLKFDPNRFENQASVPPYCFIGFGGGP 422
           +K+  + G+ IPKGW++ W    TH     F  P KFDP+RFE +   PPY F+ FGGGP
Sbjct: 381 IKEFIYDGFTIPKGWKLHWTVNSTHQKSEYFSNPEKFDPSRFEGEGP-PPYTFVPFGGGP 440

Query: 423 RICPGNEFARVETLVTIHYLITQFTWKLLSD-DHFIRDPMPVPTKGLPIRIFPRE 472
           R+CPGNEFAR+E L+ +H ++  F W L++  +  I DPMP P  GLPI++ P +
Sbjct: 441 RMCPGNEFARMEILIFLHNIVKNFNWNLVNPLEKVIVDPMPAPVNGLPIKLVPHD 493

[Close]

BLAST of Lag0038030 vs. ExPASy Swiss-Prot
Match: Q50EK0 (Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 5.7e-107
Identity = 195/476 (40.97%), Postives = 294/476 (61.76%), Query Frame = 0


Query: 3   VFFTIF---SLLISIFLFLLTGRRSS--QKLPPGSLGFPFIGQSLGLLRAMRTNTAEEWL 62
           + FT+F    + ++ F +L++  R+   + +PPG+ G+P IG++L  L   R     ++ 
Sbjct: 21  LMFTVFLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNPRDFF 80

Query: 63  QQRIRKYGPISKLTLFGKPTVFVCGQGANKLIFNGDSAVSNQQNESLRAILGESNLLELS 122
             R +KYG +   +L G PTV  C    N+ +F+ ++ +      S    L  S+L+   
Sbjct: 81  DSRTQKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENKLVVNSWPSSVGNLFRSSLITTV 140

Query: 123 GDDHKRVRGALVSFLKPECLKQYVGKMDEEIRRHIEMHWQGKQEVTVLPLMKTLTFNIVC 182
           GDD KR+R  L++FL+PE L+++VG++D   +RH+  HW GK EVT LPL+K  TF++ C
Sbjct: 141 GDDAKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVTALPLLKRYTFSLAC 200

Query: 183 SLLFGLEQGTRREKMIECFRVMIGGVWSIPINLPFTRYNQSRRASKRIQEMLKNLLDEKR 242
            L   +       ++   F V + GV  IPI+LP TRYN+++ A+  I++ L ++++E++
Sbjct: 201 DLFASINNKDDLGRLWLHFMVFVKGVMQIPIDLPGTRYNKAKHAANAIRQQLGSIINERK 260

Query: 243 VELEEKGAPPHQDLITCLLSIRNEENELLLTEKEIVHNILLVMVAGFDTSSVLITFMMRI 302
           + LE   A P QDL++ LLS  +E+ E  LT+ EI  NILL++ AG DTSS  +T +++ 
Sbjct: 261 IGLEAGNASPEQDLLSFLLSNVDEQGE-SLTDNEIQDNILLLLYAGHDTSSSTLTVLLKF 320

Query: 303 LANNPTVYAAVLQEQEEIARGKGCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRA 362
           LA NP  Y  VL+EQ +IA  K  G+LL WEDL +MKY+WRVA E LRL     G FR+A
Sbjct: 321 LAENPHCYEEVLREQLDIAGSKEAGQLLEWEDLQRMKYSWRVAQEALRLFPAAQGSFRKA 380

Query: 363 MKDIEFGGYLIPKGWQIFWASPMTHLDDTIFKEPLKFDPNRFENQASVPPYCFIGFGGGP 422
           +K+  + G+ IPKGW+++W    TH     F  P  FDP+RFE +   PPY F+ FGGGP
Sbjct: 381 IKEFIYDGFTIPKGWKMYWTVNSTHRKSEYFSNPETFDPSRFEGEGP-PPYTFVPFGGGP 440

Query: 423 RICPGNEFARVETLVTIHYLITQFTWKLLS-DDHFIRDPMPVPTKGLPIRIFPRET 473
           R+CPGNEFAR+E LV +H ++    W L++  +  I DPMP P  GLPI++ P ++
Sbjct: 441 RMCPGNEFARLEILVFLHNIVKNCKWNLVNPGEKVIVDPMPAPVNGLPIKLVPHDS 494

[Close]

BLAST of Lag0038030 vs. ExPASy Swiss-Prot
Match: A0A0S2II38 (Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=1 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.0e-103
Identity = 190/476 (39.92%), Postives = 294/476 (61.76%), Query Frame = 0


Query: 1   MIVFFTIFSLLISIFLFL--LTGRRSSQKLPPGSLGFPFIGQSLGLLRAMRTNTAEEWLQ 60
           ++  F +F  L   FLF    +G  S   LPPG  G+P IG+S   L        E+++ 
Sbjct: 8   LLSLFVLFVSLSFYFLFYKSKSGSTSGLPLPPGKTGWPVIGESFEFLSTGWKGYPEKFIF 67

Query: 61  QRIRKYGP-ISKLTLFGKPTVFVCGQGANKLIF-NGDSAVSNQQNESLRAILGESNLLEL 120
            R+ KY   + K +LFG+P    CG   NK +F N +  V     +S+  +   S     
Sbjct: 68  DRLAKYSSNVFKTSLFGQPAAAFCGAACNKFLFSNENKLVQAWWPDSVNKVFPSSTQTS- 127

Query: 121 SGDDHKRVRGALVSFLKPECLKQYVGKMDEEIRRHIEMHWQGKQEVTVLPLMKTLTFNIV 180
           S ++  ++R  L +FLKPE L++YVG MD+   +H +  W+ K+EV V PL K  TF I 
Sbjct: 128 SKEEAIKMRKLLPNFLKPEALQRYVGVMDQIATKHFKSGWENKKEVLVFPLAKNYTFWIA 187

Query: 181 CSLLFGLEQGTRREKMIECFRVMIGGVWSIPINLPFTRYNQSRRASKRIQEMLKNLLDEK 240
           C +   +E+ T+  K++E F  +  G+ S+PI+LP T +N + ++SK +++ L  ++ ++
Sbjct: 188 CKVFVSVEEPTQVAKLLEPFNAIASGIISVPIDLPGTPFNSAIKSSKIVRDKLMGIIKQR 247

Query: 241 RVELEEKGAPPHQDLITCLLSIRNEENELLLTEKEIVHNILLVMVAGFDTSSVLITFMMR 300
           + +L E  A P QD+++ +L + ++EN   +TE +I   IL +++ G DT+S   TF+++
Sbjct: 248 KTDLGEGKASPTQDILSHML-LTSDENGKFMTEGDIADKILGLLIGGHDTASSACTFVVK 307

Query: 301 ILANNPTVYAAVLQEQEEIARGKGCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRR 360
            LA  P +Y  V +EQ EIA+ K  GELL WED+ KMKY+W VA E LRL  P+ G FR 
Sbjct: 308 FLAELPEIYEGVYKEQMEIAKSKKAGELLNWEDIQKMKYSWNVACEVLRLAPPLQGAFRE 367

Query: 361 AMKDIEFGGYLIPKGWQIFWASPMTHLDDTIFKEPLKFDPNRFENQASVPPYCFIGFGGG 420
           A+ D  + G+ IPKGW+++W++  TH +  +F EPLKFDP+RFE  A  PPY F+ FGGG
Sbjct: 368 ALTDFSYNGFSIPKGWKLYWSANSTHRNSEVFPEPLKFDPSRFEG-AGPPPYSFVPFGGG 427

Query: 421 PRICPGNEFARVETLVTIHYLITQFTW-KLLSDDHFIRDPMPVPTKGLPIRIFPRE 472
           PR+CPG E+AR+E LV +H+++ +F W K++ D+  + +PMP+P KGLP+R+FP +
Sbjct: 428 PRMCPGKEYARLEILVFMHHVVKRFKWEKVIPDEKIVVNPMPIPAKGLPVRLFPHK 480

[Close]

BLAST of Lag0038030 vs. ExPASy Swiss-Prot
Match: Q2MJ20 (Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 6.8e-100
Identity = 191/474 (40.30%), Postives = 288/474 (60.76%), Query Frame = 0


Query: 1   MIVFFTIFSLLISIFLFLLTGRRSSQKLPPGSLGFPFIGQSLGLLRAMRTNTAEEWLQQR 60
           +++F T  SL  S+F F+   ++S   LPPG +G+P IG+SL  L        E+++  R
Sbjct: 10  LLLFVTFISL--SLF-FIFYKQKSPLNLPPGKMGYPIIGESLEFLSTGWKGHPEKFIFDR 69

Query: 61  IRKY-GPISKLTLFGKPTVFVCGQGANKLIF-NGDSAVSNQQNESLRAILGESNLLELSG 120
           +RKY   + K ++ G+ TV  CG  +NK +F N +  V+    +S+  I   ++L     
Sbjct: 70  MRKYSSELFKTSIVGESTVVCCGAASNKFLFSNENKLVTAWWPDSVNKIFPTTSLDSNLK 129

Query: 121 DDHKRVRGALVSFLKPECLKQYVGKMDEEIRRHIEMHWQGKQEVTVLPLMKTLTFNIVCS 180
           ++  ++R  L  F KPE L++YVG MD   +RH   HW  K E+TV PL K  TF + C 
Sbjct: 130 EESIKMRKLLPQFFKPEALQRYVGVMDVIAQRHFVTHWDNKNEITVYPLAKRYTFLLACR 189

Query: 181 LLFGLEQGTRREKMIECFRVMIGGVWSIPINLPFTRYNQSRRASKRIQEMLKNLLDEKRV 240
           L   +E      K  + F+++  G+ S+PI+LP T +N++ +AS  I++ L  ++ ++RV
Sbjct: 190 LFMSVEDENHVAKFSDPFQLIAAGIISLPIDLPGTPFNKAIKASNFIRKELIKIIKQRRV 249

Query: 241 ELEEKGAPPHQDLITCLLSIRNEENELLLTEKEIVHNILLVMVAGFDTSSVLITFMMRIL 300
           +L E  A P QD+++ +L + ++EN   + E  I   IL +++ G DT+SV  TF+++ L
Sbjct: 250 DLAEGTASPTQDILSHML-LTSDENGKSMNELNIADKILGLLIGGHDTASVACTFLVKYL 309

Query: 301 ANNPTVYAAVLQEQEEIARGKGCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRAM 360
              P +Y  V QEQ EIA+ K  GELL W+DL KMKY+W VA E +RL  P+ GGFR A+
Sbjct: 310 GELPHIYDKVYQEQMEIAKSKPAGELLNWDDLKKMKYSWNVACEVMRLSPPLQGGFREAI 369

Query: 361 KDIEFGGYLIPKGWQIFWASPMTHLDDTIFKEPLKFDPNRFENQASVPPYCFIGFGGGPR 420
            D  F G+ IPKGW+++W++  TH +   F  P KFDP RFE      PY F+ FGGGPR
Sbjct: 370 TDFMFNGFSIPKGWKLYWSANSTHKNAECFPMPEKFDPTRFEGNGPA-PYTFVPFGGGPR 429

Query: 421 ICPGNEFARVETLVTIHYLITQFTW-KLLSDDHFIRDPMPVPTKGLPIRIFPRE 472
           +CPG E+AR+E LV +H L+ +F W K++ D+  I DP P+P K LPIR++P +
Sbjct: 430 MCPGKEYARLEILVFMHNLVKRFKWEKVIPDEKIIVDPFPIPAKDLPIRLYPHK 478

[Close]

BLAST of Lag0038030 vs. ExPASy Swiss-Prot
Match: I7C6E8 (Beta-amyrin 28-monooxygenase OS=Panax ginseng OX=4054 GN=CYP716A52v2 PE=1 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 2.9e-98
Identity = 181/476 (38.03%), Postives = 288/476 (60.50%), Query Frame = 0


Query: 1   MIVFFTIFSLLISIFLFLLTGRRSSQ--KLPPGSLGFPFIGQSLGLLRAMRTNTAEEWLQ 60
           ++  F +F  L   FLF  +   SS    LPPG  G+P IG+S   L        E+++ 
Sbjct: 8   LLSLFVLFISLSFHFLFYKSKPSSSGGFPLPPGKTGWPIIGESYEFLSTGWKGYPEKFIF 67

Query: 61  QRIRKYGP-ISKLTLFGKPTVFVCGQGANKLIF-NGDSAVSNQQNESLRAILGESNLLEL 120
            R+ KY   + K ++FG+P    CG   NK +F N +  V     +S+  +   S     
Sbjct: 68  DRMTKYSSNVFKTSIFGEPAAVFCGAACNKFLFSNENKLVQAWWPDSVNKVFPSSTQTS- 127

Query: 121 SGDDHKRVRGALVSFLKPECLKQYVGKMDEEIRRHIEMHWQGKQEVTVLPLMKTLTFNIV 180
           S ++  ++R  L +F KPE L++Y+G MD+    H E  W+ K EV V PL K+ TF I 
Sbjct: 128 SKEEAIKMRKMLPNFFKPEALQRYIGLMDQIAANHFESGWENKNEVVVFPLAKSYTFWIA 187

Query: 181 CSLLFGLEQGTRREKMIECFRVMIGGVWSIPINLPFTRYNQSRRASKRIQEMLKNLLDEK 240
           C +   +E+  +  +++E F  +  G+ S+PI+LP T +N + ++SK ++  L  ++ ++
Sbjct: 188 CKVFVSVEEPAQVAELLEPFSAIASGIISVPIDLPGTPFNSAIKSSKIVRRKLVGIIKQR 247

Query: 241 RVELEEKGAPPHQDLITCLLSIRNEENELLLTEKEIVHNILLVMVAGFDTSSVLITFMMR 300
           +++L E  A   QD+++ +L + ++E+   + E +I   IL +++ G DT+S   TF+++
Sbjct: 248 KIDLGEGKASATQDILSHML-LTSDESGKFMGEGDIADKILGLLIGGHDTASSACTFVVK 307

Query: 301 ILANNPTVYAAVLQEQEEIARGKGCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRR 360
            LA  P +Y  V QEQ EI + K  GELL WED+ KMKY+W VA E LRL  P+ G FR 
Sbjct: 308 FLAELPQIYEGVYQEQMEIVKSKKAGELLKWEDIQKMKYSWNVACEVLRLAPPLQGAFRE 367

Query: 361 AMKDIEFGGYLIPKGWQIFWASPMTHLDDTIFKEPLKFDPNRFENQASVPPYCFIGFGGG 420
           A+ D  + G+ IPKGW+++W++  TH++  +F EPLKFDP+RF+  A  PP+ F+ FGGG
Sbjct: 368 ALSDFTYNGFSIPKGWKLYWSANSTHINSEVFPEPLKFDPSRFDG-AGPPPFSFVPFGGG 427

Query: 421 PRICPGNEFARVETLVTIHYLITQFTW-KLLSDDHFIRDPMPVPTKGLPIRIFPRE 472
           PR+CPG E+AR+E LV +H+L+ +F W K++ D+  + +PMP+P  GLP+R+FP +
Sbjct: 428 PRMCPGKEYARLEILVFMHHLVKRFKWEKVIPDEKIVVNPMPIPANGLPVRLFPHK 480

[Close]

BLAST of Lag0038030 vs. ExPASy TrEMBL
Match: A0A6J1CC35 (LOW QUALITY PROTEIN: cytochrome P450 716B1-like OS=Momordica charantia OX=3673 GN=LOC111010306 PE=3 SV=1)

HSP 1 Score: 857.8 bits (2215), Expect = 2.1e-245
Identity = 419/478 (87.66%), Postives = 449/478 (93.93%), Query Frame = 0


Query: 1   MIVFFTIFSLLISIFLFLLTGR--RSSQKLPPGSLGFPFIGQSLGLLRAMRTNTAEEWLQ 60
           M ++ TIFS LI IFLFL  GR  RSSQKLPPGSLG PFIGQSLGLLRAMR+NTAE+WLQ
Sbjct: 1   MNIYLTIFSFLIPIFLFLTRGRRSRSSQKLPPGSLGVPFIGQSLGLLRAMRSNTAEQWLQ 60

Query: 61  QRIRKYGPISKLTLFGKPTVFVCGQGANKLIFNGDSAVSNQQNESLRAILGESNLLELSG 120
           +RI KYGPISKLTLFGKPTV V GQGANKLIFNGD+A+SNQQNESLRAILGESNLLEL+G
Sbjct: 61  RRITKYGPISKLTLFGKPTVLVGGQGANKLIFNGDTAISNQQNESLRAILGESNLLELTG 120

Query: 121 DDHKRVRGALVSFLKPECLKQYVGKMDEEIRRHIEMHWQGKQEVTVLPLMKTLTFNIVCS 180
           DDHKRVR ALV FLKPECLKQYVGKMDEE+RRH+EMHWQ KQEVTVLPLMKTLTFNIVCS
Sbjct: 121 DDHKRVRSALVXFLKPECLKQYVGKMDEEVRRHLEMHWQXKQEVTVLPLMKTLTFNIVCS 180

Query: 181 LLFGLEQGTRREKMIECFRVMIGGVWSIPINLPFTRYNQSRRASKRIQEMLKNLLDEKRV 240
           LLFGLE+GTRRE+MIECF+VMIGGVWSIPINLPFTRYNQSR+AS +IQEMLKNLLDEKRV
Sbjct: 181 LLFGLEEGTRRERMIECFQVMIGGVWSIPINLPFTRYNQSRQASIKIQEMLKNLLDEKRV 240

Query: 241 ELEEKGAPPHQDLITCLLSIRNEENELLLTEKEIVHNILLVMVAGFDTSSVLITFMMRIL 300
           ELE KG  PHQDLITCLLSI+N+E+E +LTEKEI+HNI+LVMVAG+DTSSVLITFM+R L
Sbjct: 241 ELEVKGTSPHQDLITCLLSIKNKEHEQVLTEKEIIHNIILVMVAGYDTSSVLITFMVRFL 300

Query: 301 ANNPTVYAAVLQEQEEIARGKGCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRAM 360
           ANNPTVYAAVLQEQEEIAR K CGELLTWEDLAKMKYTWRVALETLRLVAP+FGGFR+AM
Sbjct: 301 ANNPTVYAAVLQEQEEIARSKECGELLTWEDLAKMKYTWRVALETLRLVAPVFGGFRKAM 360

Query: 361 KDIEFGGYLIPKGWQIFWASPMTHLDDTIFKEPLKFDPNRFENQASVPPYCFIGFGGGPR 420
           KDIEFGGYLIPKGWQIFWASPMTHLDDTIF+EPLKFDPNRFENQASVP YCF+ FGGGPR
Sbjct: 361 KDIEFGGYLIPKGWQIFWASPMTHLDDTIFEEPLKFDPNRFENQASVPXYCFVAFGGGPR 420

Query: 421 ICPGNEFARVETLVTIHYLITQFTWKLLSDDHFIRDPMPVPTKGLPIRIFPRETSMSL 477
           ICPG EFARVETLVTIHYLITQFTWKLL DD FIRDPMPVPTKGLPI++ PRETSM+L
Sbjct: 421 ICPGYEFARVETLVTIHYLITQFTWKLLLDDLFIRDPMPVPTKGLPIKVCPRETSMTL 478

[Close]

BLAST of Lag0038030 vs. ExPASy TrEMBL
Match: A0A5A7TZG7 (Cytochrome P450 716B1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold363G00020 PE=3 SV=1)

HSP 1 Score: 823.2 bits (2125), Expect = 5.7e-235
Identity = 405/477 (84.91%), Postives = 434/477 (90.99%), Query Frame = 0


Query: 1   MIVFFTI-FSLLISIFLFLLTGRRSSQKLPPGSLGFPFIGQSLGLLRAMRTNTAEEWLQQ 60
           MI++ TI F LLI IFLFL T  +  Q LPPGSLGFPFIGQSL LLRAMRTNTAE+W QQ
Sbjct: 1   MIIYLTILFCLLIPIFLFLRTSSK-KQHLPPGSLGFPFIGQSLSLLRAMRTNTAEQWAQQ 60

Query: 61  RIRKYGPISKLTLFGKPTVFVCGQGANKLIFNGDSA-VSNQQNESLRAILGESNLLELSG 120
           RIR YG ISKLTLFGKPTVF+ GQ  NKLIF+GD A VSNQQNESLRAILGE NLLEL+G
Sbjct: 61  RIRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELTG 120

Query: 121 DDHKRVRGALVSFLKPECLKQYVGKMDEEIRRHIEMHWQGKQEVTVLPLMKTLTFNIVCS 180
           +DHKRVR ALVSFLKPECLK+YVGKMDEEIR HI MHW  KQEVTVLPLMKTLTFNIVCS
Sbjct: 121 EDHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIHMHWHRKQEVTVLPLMKTLTFNIVCS 180

Query: 181 LLFGLEQGTRREKMIECFRVMIGGVWSIPINLPFTRYNQSRRASKRIQEMLKNLLDEKRV 240
           LLFGLEQG RRE+MIECFRVMI GVWSIPINLPFTRYNQSRRAS++IQEMLK LLDEKRV
Sbjct: 181 LLFGLEQGMRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRV 240

Query: 241 ELEEKGAPPHQDLITCLLSIRNEENELLLTEKEIVHNILLVMVAGFDTSSVLITFMMRIL 300
           ELEEKG   HQDLITCLLSIRNEENEL+L+EKEIVHNI+LVMVAGFDTSSVLITFMMR L
Sbjct: 241 ELEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRSL 300

Query: 301 ANNPTVYAAVLQEQEEIARGKGCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRAM 360
           ANNPTVYAAVLQEQEEIAR K CGELL WEDLAKMKYTWRVALETLRLVAPIFGGFR+AM
Sbjct: 301 ANNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAM 360

Query: 361 KDIEFGGYLIPKGWQIFWASPMTHLDDTIFKEPLKFDPNRFENQASVPPYCFIGFGGGPR 420
            DI+FGGYLIPKGWQIFW SP+THLDD IF+EP KFDP+RF+N AS+PPYCF+GFG GPR
Sbjct: 361 NDIQFGGYLIPKGWQIFWTSPVTHLDDNIFREPSKFDPDRFKNPASIPPYCFLGFGSGPR 420

Query: 421 ICPGNEFARVETLVTIHYLITQFTWKLLSDDHFIRDPMPVPTKGLPIRIFPRETSMS 476
           ICPGNEFARVETLVTIHYLITQFTWKL+SDDHFIRDPMP PTKG+PI+I+PR+T M+
Sbjct: 421 ICPGNEFARVETLVTIHYLITQFTWKLISDDHFIRDPMPTPTKGMPIKIWPRQTFMA 476

[Close]

BLAST of Lag0038030 vs. ExPASy TrEMBL
Match: A0A1S3C4V0 (cytochrome P450 716B1-like OS=Cucumis melo OX=3656 GN=LOC103496862 PE=3 SV=1)

HSP 1 Score: 823.2 bits (2125), Expect = 5.7e-235
Identity = 405/477 (84.91%), Postives = 434/477 (90.99%), Query Frame = 0


Query: 1   MIVFFTI-FSLLISIFLFLLTGRRSSQKLPPGSLGFPFIGQSLGLLRAMRTNTAEEWLQQ 60
           MI++ TI F LLI IFLFL T  +  Q LPPGSLGFPFIGQSL LLRAMRTNTAE+W QQ
Sbjct: 1   MIIYLTILFCLLIPIFLFLRTSSK-KQHLPPGSLGFPFIGQSLSLLRAMRTNTAEQWAQQ 60

Query: 61  RIRKYGPISKLTLFGKPTVFVCGQGANKLIFNGDSA-VSNQQNESLRAILGESNLLELSG 120
           RIR YG ISKLTLFGKPTVF+ GQ  NKLIF+GD A VSNQQNESLRAILGE NLLEL+G
Sbjct: 61  RIRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELTG 120

Query: 121 DDHKRVRGALVSFLKPECLKQYVGKMDEEIRRHIEMHWQGKQEVTVLPLMKTLTFNIVCS 180
           +DHKRVR ALVSFLKPECLK+YVGKMDEEIR HI MHW  KQEVTVLPLMKTLTFNIVCS
Sbjct: 121 EDHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIHMHWHRKQEVTVLPLMKTLTFNIVCS 180

Query: 181 LLFGLEQGTRREKMIECFRVMIGGVWSIPINLPFTRYNQSRRASKRIQEMLKNLLDEKRV 240
           LLFGLEQG RRE+MIECFRVMI GVWSIPINLPFTRYNQSRRAS++IQEMLK LLDEKRV
Sbjct: 181 LLFGLEQGMRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRV 240

Query: 241 ELEEKGAPPHQDLITCLLSIRNEENELLLTEKEIVHNILLVMVAGFDTSSVLITFMMRIL 300
           ELEEKG   HQDLITCLLSIRNEENEL+L+EKEIVHNI+LVMVAGFDTSSVLITFMMR L
Sbjct: 241 ELEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRSL 300

Query: 301 ANNPTVYAAVLQEQEEIARGKGCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRAM 360
           ANNPTVYAAVLQEQEEIAR K CGELL WEDLAKMKYTWRVALETLRLVAPIFGGFR+AM
Sbjct: 301 ANNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAM 360

Query: 361 KDIEFGGYLIPKGWQIFWASPMTHLDDTIFKEPLKFDPNRFENQASVPPYCFIGFGGGPR 420
            DI+FGGYLIPKGWQIFW SP+THLDD IF+EP KFDP+RF+N AS+PPYCF+GFG GPR
Sbjct: 361 NDIQFGGYLIPKGWQIFWTSPVTHLDDNIFREPSKFDPDRFKNPASIPPYCFLGFGSGPR 420

Query: 421 ICPGNEFARVETLVTIHYLITQFTWKLLSDDHFIRDPMPVPTKGLPIRIFPRETSMS 476
           ICPGNEFARVETLVTIHYLITQFTWKL+SDDHFIRDPMP PTKG+PI+I+PR+T M+
Sbjct: 421 ICPGNEFARVETLVTIHYLITQFTWKLISDDHFIRDPMPTPTKGMPIKIWPRQTFMA 476

[Close]

BLAST of Lag0038030 vs. ExPASy TrEMBL
Match: A0A0A0LA02 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G186690 PE=3 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 2.4e-233
Identity = 407/477 (85.32%), Postives = 433/477 (90.78%), Query Frame = 0


Query: 1   MIVFFTI-FSLLISIFLFLLTGRRSSQK--LPPGSLGFPFIGQSLGLLRAMRTNTAEEWL 60
           MI++ TI F LLI IFLFL   RRSS+K  LPPGSLGFP IGQSL LLRAMRTNTAE+W 
Sbjct: 1   MIIYLTIFFCLLIPIFLFL---RRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWA 60

Query: 61  QQRIRKYGPISKLTLFGKPTVFVCGQGANKLIFNGDSA-VSNQQNESLRAILGESNLLEL 120
           QQRIR YG ISKLTLFGKPTVF+ GQ  NKLIF+GD A VSNQQNESLRAILGE NLLEL
Sbjct: 61  QQRIRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLEL 120

Query: 121 SGDDHKRVRGALVSFLKPECLKQYVGKMDEEIRRHIEMHWQGKQEVTVLPLMKTLTFNIV 180
            G+DHKRVR ALVSFLKPECLK+YVGKMDEEIR HI MHW  KQEVTVLPLMKTLTFNIV
Sbjct: 121 IGEDHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIV 180

Query: 181 CSLLFGLEQGTRREKMIECFRVMIGGVWSIPINLPFTRYNQSRRASKRIQEMLKNLLDEK 240
           CSLLFGLEQGTRRE+MIECFRVMI GVWSIPINLPFTRYNQSRRAS++IQEMLK LLDEK
Sbjct: 181 CSLLFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEK 240

Query: 241 RVELEEKGAPPHQDLITCLLSIRNEENELLLTEKEIVHNILLVMVAGFDTSSVLITFMMR 300
           RVELEEKG   HQDLITCLLSIRNEENEL+L+EKEIVHNI+LV+VAGFDTSSVLITFMMR
Sbjct: 241 RVELEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMR 300

Query: 301 ILANNPTVYAAVLQEQEEIARGKGCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRR 360
            LANNPTVYAAVLQEQEEIAR K CGELL WEDLAKMKYTWRVALETLRLVAPIFGGFR+
Sbjct: 301 NLANNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRK 360

Query: 361 AMKDIEFGGYLIPKGWQIFWASPMTHLDDTIFKEPLKFDPNRFENQASVPPYCFIGFGGG 420
           AM DI+FGGYLIPKGWQIFW SP+THLDDTIF+EP KFDP+RF+N AS+PPYCF+GFG G
Sbjct: 361 AMNDIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSG 420

Query: 421 PRICPGNEFARVETLVTIHYLITQFTWKLLSDDHFIRDPMPVPTKGLPIRIFPRETS 474
           PRICPGNEFARVETLVTIHYLITQFTWKLL DDHFIRDPMP PTKG+PI+I+PR  S
Sbjct: 421 PRICPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQS 474

[Close]

BLAST of Lag0038030 vs. ExPASy TrEMBL
Match: A0A5J5A0L0 (Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_010117 PE=3 SV=1)

HSP 1 Score: 735.7 bits (1898), Expect = 1.2e-208
Identity = 346/473 (73.15%), Postives = 416/473 (87.95%), Query Frame = 0


Query: 1   MIVFFTIFSLLISIFLFLLTGRRSSQKLPPGSLGFPFIGQSLGLLRAMRTNTAEEWLQQR 60
           M +FFTIF  L+ IF  L+  R SSQ+LPPGSLG P IGQSL LL+AM+ NTAE WL++R
Sbjct: 15  MNIFFTIFLFLLPIFFLLVRRRSSSQRLPPGSLGIPIIGQSLSLLQAMKANTAERWLEER 74

Query: 61  IRKYGPISKLTLFGKPTVFVCGQGANKLIFNGD-SAVSNQQNESLRAILGESNLLELSGD 120
           IRKYG ISKL+LFGKPTVF+ GQ ANKL+F GD S ++NQQ +S+R ILG+ NLLELSG+
Sbjct: 75  IRKYGSISKLSLFGKPTVFIYGQAANKLVFTGDCSVITNQQAQSVRMILGDRNLLELSGE 134

Query: 121 DHKRVRGALVSFLKPECLKQYVGKMDEEIRRHIEMHWQGKQEVTVLPLMKTLTFNIVCSL 180
           DHKRVR ALVSFLKPECLKQYVGKM+EEIR H+EMHW+GKQ+VTVLPLMKTLTFNI+CSL
Sbjct: 135 DHKRVRDALVSFLKPECLKQYVGKMEEEIREHLEMHWEGKQKVTVLPLMKTLTFNIICSL 194

Query: 181 LFGLEQGTRREKMIECFRVMIGGVWSIPINLPFTRYNQSRRASKRIQEMLKNLLDEKRVE 240
           LFG+++G RR+K++E F+ MI G+WS+P+NLPFTRYN+S RA+ R+Q MLK+L+ EKRVE
Sbjct: 195 LFGVQRGARRDKLVEYFQEMIQGMWSVPVNLPFTRYNRSLRATARVQNMLKDLIREKRVE 254

Query: 241 LEEKGAPPHQDLITCLLSIRNEENELLLTEKEIVHNILLVMVAGFDTSSVLITFMMRILA 300
           LE++GA  HQDLITCLLSIR ++NE L++EKEIVHN++LVMVAG DTSSVLI+F+MR+L+
Sbjct: 255 LEQQGASSHQDLITCLLSIRGKDNEELISEKEIVHNVMLVMVAGHDTSSVLISFIMRLLS 314

Query: 301 NNPTVYAAVLQEQEEIARGKGCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRAMK 360
           N+PTVYAAVLQEQEEIA+ K CGELLTWEDLAKMKYTWR A+ETLR+  PIFGGFR+ +K
Sbjct: 315 NDPTVYAAVLQEQEEIAKSKPCGELLTWEDLAKMKYTWRAAMETLRMFPPIFGGFRKTLK 374

Query: 361 DIEFGGYLIPKGWQIFWASPMTHLDDTIFKEPLKFDPNRFENQASVPPYCFIGFGGGPRI 420
           DIE+ GYLIPKGWQIFWAS MTH+D +IF++P KFDP RFENQASVPPYCF+ FGGGPRI
Sbjct: 375 DIEYKGYLIPKGWQIFWASSMTHMDSSIFQDPSKFDPTRFENQASVPPYCFVAFGGGPRI 434

Query: 421 CPGNEFARVETLVTIHYLITQFTWKL-LSDDHFIRDPMPVPTKGLPIRIFPRE 472
           CPG EFAR+ETLVT+HYL+T+FTWKL  +D+ F RDPMPVPT+GLPI I P++
Sbjct: 435 CPGYEFARIETLVTVHYLVTRFTWKLCCTDNFFSRDPMPVPTEGLPIEIVPKK 487

[Close]

BLAST of Lag0038030 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 345.5 bits (885), Expect = 6.8e-95
Identity = 181/479 (37.79%), Postives = 290/479 (60.54%), Query Frame = 0


Query: 1   MIVFFTIFSLLISIFLFLL--TGRRSSQKLPPGSLGFPFIGQSLGLLRAMRTNTAEEWLQ 60
           MI+ F + S+L+S+ L L       S   LPPG+ G P IG+S   L A R    E+++ 
Sbjct: 6   MIILF-LSSILLSLLLLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHPEKFIT 65

Query: 61  QRIRKYGPIS----KLTLFGKPTVFVCGQGANKLIF-NGDSAVSNQQNESLRAILGESNL 120
            R+R++   S    K  LFG PT  V G   NK +F N +  V +   +S+  I   S++
Sbjct: 66  DRVRRFSSSSSCVFKTHLFGSPTAVVTGASGNKFLFTNENKLVVSWWPDSVNKIF-PSSM 125

Query: 121 LELSGDDHKRVRGALVSFLKPECLKQYVGKMDEEIRRHIEMHWQGKQEVTVLPLMKTLTF 180
              S ++ +++R  L  F+KPE L++YVG MDE  +RH E  W  + +V V PL K  TF
Sbjct: 126 QTSSKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTKKFTF 185

Query: 181 NIVCSLLFGLEQGTRREKMIECFRVMIGGVWSIPINLPFTRYNQSRRASKRIQEMLKNLL 240
           +I C     +E   R  ++ E F  +  G++SIPI+LP TR+N++ +AS+ +++ +  ++
Sbjct: 186 SIACRSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEVSAIV 245

Query: 241 DEKRVELEEKGAPPHQDLITCLLSIRNEENELLLTEKEIVHNILLVMVAGFDTSSVLITF 300
            +++ EL+   A    D+++ +L    E       ++++   I+ +++ G DT+S++ TF
Sbjct: 246 RQRKEELKAGKALEEHDILSHMLMNIGETK-----DEDLADKIIGLLIGGHDTASIVCTF 305

Query: 301 MMRILANNPTVYAAVLQEQEEIARGKGCGELLTWEDLAKMKYTWRVALETLRLVAPIFGG 360
           ++  LA  P VY  VLQEQ+EI + K   E L WED+ KM+Y+W VA E +R+V P+ G 
Sbjct: 306 VVNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVPPLSGT 365

Query: 361 FRRAMKDIEFGGYLIPKGWQIFWASPMTHLDDTIFKEPLKFDPNRFENQASVPPYCFIGF 420
           FR A+    F G+ IPKGW+++W++  TH++   F EP +F+PNRFE  +   PY ++ F
Sbjct: 366 FREAIDHFSFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEG-SGPKPYTYVPF 425

Query: 421 GGGPRICPGNEFARVETLVTIHYLITQFTWKLL--SDDHFIRDPMPVPTKGLPIRIFPR 471
           GGGPR+CPG E+AR+E L+ +H L+ +F W+ +  +++  + DP+P+P KGLPIRIFP+
Sbjct: 426 GGGPRMCPGKEYARLEILIFMHNLVNRFKWEKVFPNENKIVVDPLPIPDKGLPIRIFPQ 476

[Close]

BLAST of Lag0038030 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 323.9 bits (829), Expect = 2.1e-88
Identity = 165/458 (36.03%), Postives = 283/458 (61.79%), Query Frame = 0


Query: 17  FLLTGRRSSQKLPPGSLGFPFIGQSLGLLRAMRTNTA-EEWLQQRIRKYGPISKLTLFGK 76
           F+    +  +KL PG +G P+IG+++   +A ++N   E+++  RI K+G I K  + G 
Sbjct: 34  FITKKIQKKKKLLPGEMGLPWIGETMDFYKAQKSNRVFEDFVNPRIIKHGNIFKTRIMGS 93

Query: 77  PTVFVCGQGANKLIFNGD-SAVSNQQNESLRAILGESNLLELSGDDHKRVRGALVSFLKP 136
           PT+ V G  AN+LI + + S V +    S   ++G + ++   G+ H+ +RG + + L  
Sbjct: 94  PTIVVNGAEANRLILSNEFSLVVSSWPSSSVQLMGMNCIMAKQGEKHRVLRGIVANSLSY 153

Query: 137 ECLKQYVGKMDEEIRRHIEMHWQGKQEVTVLPLMKTLTFNIVCSLLFGLEQGTRREKMIE 196
             L+  + K+ + ++ H E  W+GK+E+++    K LTF +V   L+G++       M+E
Sbjct: 154 IGLESLIPKLCDTVKFHHETEWRGKEEISLYRSAKVLTFTVVFECLYGIKV---EIGMLE 213

Query: 197 CFRVMIGGVWSIPINLPFTRYNQSRRASKRIQEMLKNLLDEKRVELEEKGA-PPHQDLIT 256
            F  ++ GV+++P+  P +++ ++++A   I+  L   + EKR E+E++GA  P+  L +
Sbjct: 214 VFERVLEGVFALPVEFPCSKFARAKKARLEIETFLVGKVREKRREMEKEGAEKPNTTLFS 273

Query: 257 CLLSIRNEENELLLTEKEIVHNILLVMVAGFDTSSVLITFMMRILANNPTVYAAVLQEQE 316
            L+    E  + ++TE+E+V N++L++ A  DT+S  ++   ++LA +PT    +LQE  
Sbjct: 274 RLV---EELIKGVITEEEVVDNMVLLVFAAHDTTSYAMSMTFKMLAQHPTCRDTLLQEHA 333

Query: 317 EIARGKGCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRAMKDIEFGGYLIPKGWQ 376
           +I   KG GE LT ED+ KMKY+W+V  ET+RL  PIFG FR+A+ DI++GGY IPKGW+
Sbjct: 334 QIKANKGEGEYLTVEDVKKMKYSWQVVRETMRLSPPIFGSFRKAVADIDYGGYTIPKGWK 393

Query: 377 IFWASPMTHLDDTIFKEPLKFDPNRFENQASVPPYCFIGFGGGPRICPGNEFARVETLVT 436
           I W +  TH +  IF++P+ FDP RF+    +  Y ++ FGGGPR+C G++ A++  LV 
Sbjct: 394 ILWTTYGTHYNPEIFQDPMSFDPTRFDK--PIQAYTYLPFGGGPRLCAGHQLAKISILVF 453

Query: 437 IHYLITQFTWKLLSDDHFI-RDPMPVPTKGLPIRIFPR 471
           +H+++T F W L+  D  I  DP+P P+ G+PI+I P+
Sbjct: 454 MHFVVTGFDWSLVYPDETISMDPLPFPSLGMPIKISPK 483

[Close]

BLAST of Lag0038030 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 251.9 bits (642), Expect = 1.0e-66
Identity = 155/476 (32.56%), Postives = 254/476 (53.36%), Query Frame = 0


Query: 5   FTIFSLLISIFL--FLLTGRRSSQK---LPPGSLGFPFIGQSLGLLRAMRTNTAEEWLQQ 64
           FT F LL+S     FLL  RR+  +   LPPGSLG P IG++  L+ A +T   E ++ +
Sbjct: 3   FTAFLLLLSSIAAGFLLLLRRTRYRRMGLPPGSLGLPLIGETFQLIGAYKTENPEPFIDE 62

Query: 65  RIRKYGPISKLTLFGKPTVFVCGQGANKLIFNGDSAVSN-QQNESLRAILGESNLLELSG 124
           R+ +YG +    LFG+PT+F      N+ +   +  +       S+  +LG+ +LL + G
Sbjct: 63  RVARYGSVFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSLLLMKG 122

Query: 125 DDHKRVRGALVSFLKPECLKQYVG-KMDEEIRRHIEMHWQGKQEVTVLPLMKTLTFNIVC 184
             HKR+    +SF     +K ++   +D  +R +++  W  +  V ++   K +TF +  
Sbjct: 123 SLHKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLD-SWSSR--VLLMEEAKKITFELTV 182

Query: 185 SLLFGLEQGTRREKMIECFRVMIGGVWSIPINLPFTRYNQSRRASKRIQEMLKNLLDEKR 244
             L   + G   E + + + ++I G +S+P+ L  T Y ++ +A +++ E L  ++  KR
Sbjct: 183 KQLMSFDPGEWSESLRKEYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEAL-TVVVMKR 242

Query: 245 VELEEKGAPPHQDLITCLLSIRNEENELLLTEKEIVHNILLVMVAGFDTSSVLITFMMRI 304
            E EE+GA   +D++  LL+  +       +++EIV  ++ ++VAG++T+S ++T  ++ 
Sbjct: 243 REEEEEGAERKKDMLAALLAADDG-----FSDEEIVDFLVALLVAGYETTSTIMTLAVKF 302

Query: 305 LANNPTVYAAVLQEQEEIARGKGCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRA 364
           L   P   A + +E E+I   K     L W D   M +T  V  ETLR+   I G FRRA
Sbjct: 303 LTETPLALAQLKEEHEKIRAMKSDSYSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRA 362

Query: 365 MKDIEFGGYLIPKGWQIFWASPMTHLDDTIFKEPLKFDPNRFENQA--SVPPYCFIGFGG 424
           M D+E  GY IPKGW++F +    HLD   FK+   F+P R+++ +  + P   F  FGG
Sbjct: 363 MTDVEIKGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQSNSVTTGPSNVFTPFGG 422

Query: 425 GPRICPGNEFARVETLVTIHYLITQFTWKLLSDDHFIRDPMPVPTKGLPIRIFPRE 472
           GPR+CPG E ARV   V +H L+T F+W     D  +  P     K  PI +  R+
Sbjct: 423 GPRLCPGYELARVALSVFLHRLVTGFSWVPAEQDKLVFFPTTRTQKRYPIFVKRRD 469

[Close]

BLAST of Lag0038030 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 251.1 bits (640), Expect = 1.7e-66
Identity = 153/470 (32.55%), Postives = 250/470 (53.19%), Query Frame = 0


Query: 3   VFFTIF--SLLISIFLFLLTGRR---SSQKLPPGSLGFPFIGQSLGLLRAMRTNTAEEWL 62
           +F T+F  SL +     L++ RR   S   LPPG++G+P++G++  L     +     + 
Sbjct: 6   LFLTLFAGSLFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLY----SQDPNVFF 65

Query: 63  QQRIRKYGPISKLTLFGKPTVFVCGQGANKLIFNGDSAVSNQQ-NESLRAILGESNLLEL 122
           Q + ++YG + K  + G P V +    A K +    S +       S   +LG+  +   
Sbjct: 66  QSKQKRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFH 125

Query: 123 SGDDHKRVRGALVSFLKPECLKQYVGKMDEEIRRHIEMHWQGKQEVTVLPLMKTLTFNIV 182
            GD H ++R  ++    PE ++  V  + E I +     W+G   +     MKT TFN+ 
Sbjct: 126 QGDYHAKLRKLVLRAFMPESIRNMVPDI-ESIAQDSLRSWEGTM-INTYQEMKTYTFNVA 185

Query: 183 CSLLFGLEQGTRREKMIECFRVMIGGVWSIPINLPFTRYNQSRRASKRIQEMLKNLLDEK 242
              +FG ++   RE +  C+ ++  G  S+P+NLP T +++S +A K + ++L  +L E+
Sbjct: 186 LLSIFGKDEVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSER 245

Query: 243 RVELEEKGAPPHQDLITCLLSIRNEENELLLTEKEIVHNILLVMVAGFDTSSVLITFMMR 302
           R     +    H DL+   +  + E     LT+++I  NI+ V+ A  DT++ +++++++
Sbjct: 246 R-----QNGSSHNDLLGSFMGDKEE-----LTDEQIADNIIGVIFAARDTTASVMSWILK 305

Query: 303 ILANNPTVYAAVLQEQEEIARGKGCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRR 362
            LA NP V  AV +EQ  I + K  GE LTW D  KM  T RV  ETLR+ + +   FR 
Sbjct: 306 YLAENPNVLEAVTEEQMAIRKDKEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFRE 365

Query: 363 AMKDIEFGGYLIPKGWQIFWASPMTHLDDTIFKEPLKFDPNRFENQASVPPYCFIGFGGG 422
           A++D+E+ GYLIPKGW++       H    IF  P KFDP+RFE   +  P  F+ FG G
Sbjct: 366 AVEDVEYEGYLIPKGWKVLPLFRNIHHSADIFSNPGKFDPSRFE--VAPKPNTFMPFGNG 425

Query: 423 PRICPGNEFARVETLVTIHYLITQFTWKLL-SDDHFIRDPMPVPTKGLPI 466
              CPGNE A++E  + IH+L T+++W ++ + D     P  +P  GLPI
Sbjct: 426 THSCPGNELAKLEMSIMIHHLTTKYSWSIVGASDGIQYGPFALPQNGLPI 457

[Close]

BLAST of Lag0038030 vs. TAIR 10
Match: AT4G19230.2 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 236.5 bits (602), Expect = 4.4e-62
Identity = 145/445 (32.58%), Postives = 237/445 (53.26%), Query Frame = 0


Query: 3   VFFTIF--SLLISIFLFLLTGRR---SSQKLPPGSLGFPFIGQSLGLLRAMRTNTAEEWL 62
           +F T+F  SL +     L++ RR   S   LPPG++G+P++G++  L     +     + 
Sbjct: 6   LFLTLFAGSLFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLY----SQDPNVFF 65

Query: 63  QQRIRKYGPISKLTLFGKPTVFVCGQGANKLIFNGDSAVSNQQ-NESLRAILGESNLLEL 122
           Q + ++YG + K  + G P V +    A K +    S +       S   +LG+  +   
Sbjct: 66  QSKQKRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFH 125

Query: 123 SGDDHKRVRGALVSFLKPECLKQYVGKMDEEIRRHIEMHWQGKQEVTVLPLMKTLTFNIV 182
            GD H ++R  ++    PE ++  V  + E I +     W+G   +     MKT TFN+ 
Sbjct: 126 QGDYHAKLRKLVLRAFMPESIRNMVPDI-ESIAQDSLRSWEGTM-INTYQEMKTYTFNVA 185

Query: 183 CSLLFGLEQGTRREKMIECFRVMIGGVWSIPINLPFTRYNQSRRASKRIQEMLKNLLDEK 242
              +FG ++   RE +  C+ ++  G  S+P+NLP T +++S +A K + ++L  +L E+
Sbjct: 186 LLSIFGKDEVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSER 245

Query: 243 RVELEEKGAPPHQDLITCLLSIRNEENELLLTEKEIVHNILLVMVAGFDTSSVLITFMMR 302
           R     +    H DL+   +  + E     LT+++I  NI+ V+ A  DT++ +++++++
Sbjct: 246 R-----QNGSSHNDLLGSFMGDKEE-----LTDEQIADNIIGVIFAARDTTASVMSWILK 305

Query: 303 ILANNPTVYAAVLQEQEEIARGKGCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRR 362
            LA NP V  AV +EQ  I + K  GE LTW D  KM  T RV  ETLR+ + +   FR 
Sbjct: 306 YLAENPNVLEAVTEEQMAIRKDKEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFRE 365

Query: 363 AMKDIEFGGYLIPKGWQIFWASPMTHLDDTIFKEPLKFDPNRFENQASVPPYCFIGFGGG 422
           A++D+E+ GYLIPKGW++       H    IF  P KFDP+RFE   +  P  F+ FG G
Sbjct: 366 AVEDVEYEGYLIPKGWKVLPLFRNIHHSADIFSNPGKFDPSRFE--VAPKPNTFMPFGNG 425

Query: 423 PRICPGNEFARVETLVTIHYLITQF 442
              CPGNE A++E  + IH+L T++
Sbjct: 426 THSCPGNELAKLEMSIMIHHLTTKY 432

The following BLAST results are available for this feature:

BLAST of Lag0038030 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139355.1	4.3e-245	87.66	LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Momordica charantia]	[more]
XP_038899130.1	3.9e-238	85.09	LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Benincasa hispida]	[more]
XP_008457111.1	1.2e-234	84.91	PREDICTED: cytochrome P450 716B1-like [Cucumis melo] >KAA0047356.1 cytochrome P4...	[more]
XP_004140824.1	5.0e-233	85.32	cytochrome P450 716B1 [Cucumis sativus] >KGN57442.1 hypothetical protein Csa_010...	[more]
KAA8523694.1	2.5e-208	73.15	hypothetical protein F0562_010117 [Nyssa sinensis]	[more]

BLAST of Lag0038030 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q50EK1	1.2e-107	41.05	Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1	[more]
Q50EK0	5.7e-107	40.97	Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1	[more]
A0A0S2II38	1.0e-103	39.92	Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=...	[more]
Q2MJ20	6.8e-100	40.30	Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV...	[more]
I7C6E8	2.9e-98	38.03	Beta-amyrin 28-monooxygenase OS=Panax ginseng OX=4054 GN=CYP716A52v2 PE=1 SV=1	[more]

BLAST of Lag0038030 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CC35	2.1e-245	87.66	LOW QUALITY PROTEIN: cytochrome P450 716B1-like OS=Momordica charantia OX=3673 G...	[more]
A0A5A7TZG7	5.7e-235	84.91	Cytochrome P450 716B1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3C4V0	5.7e-235	84.91	cytochrome P450 716B1-like OS=Cucumis melo OX=3656 GN=LOC103496862 PE=3 SV=1	[more]
A0A0A0LA02	2.4e-233	85.32	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G186690 PE=3 SV=1	[more]
A0A5J5A0L0	1.2e-208	73.15	Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_010117 PE=3 SV=1	[more]

BLAST of Lag0038030 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G36110.1	6.8e-95	37.79	cytochrome P450, family 716, subfamily A, polypeptide 1	[more]
AT2G42850.1	2.1e-88	36.03	cytochrome P450, family 718	[more]
AT5G05690.1	1.0e-66	32.56	Cytochrome P450 superfamily protein	[more]
AT4G19230.1	1.7e-66	32.55	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]
AT4G19230.2	4.4e-62	32.58	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 215..235
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 8..450 e-value: 9.2E-10 score: 35.8
None	No IPR available	PANTHER	PTHR24286:SF237	OS07G0520300 PROTEIN	coord: 10..469
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 10..469
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 271..288 score: 27.78 coord: 410..420 score: 48.5 coord: 420..443 score: 27.13 coord: 61..80 score: 29.42 coord: 171..189 score: 22.73 coord: 291..317 score: 18.98 coord: 377..401 score: 28.25
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 338..349 score: 34.88 coord: 411..420 score: 53.36 coord: 420..431 score: 38.15 coord: 282..299 score: 30.89
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 29..450 e-value: 1.0E-68 score: 232.2
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 20..468 e-value: 5.4E-119 score: 399.9
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 23..470
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 413..422

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0038030.1	Lag0038030.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen