Lag0012806 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0012806
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Eukaryotic aspartyl protease family protein
Location	chr1: 44510690 .. 44512213 (+)
RNA-Seq Expression	Lag0012806
Synteny	Lag0012806

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATTTATGTTGGTAGAAAACGTGGCATGCAGTGTGAACTCGGTGGATTGCAGAGGCCACCAAACTTGTCAATTTACTCTGCTACTGCTTCCATTAACGACTCATCCAAGCTTTTCCAACCAAAACCTCCATGGAGTTTCTCCCCATTCCCTTTCTCTTTTCCATCTTTCTCCTTCTTTCCACTTCATCTTCTTCCTCCAGCTCCCCATCACACTCCCCCTCACCACCTTCCCTTCACTTCCACTTACAGATCCATGGCAGAACATCAATTACCTCGTCTCTGCTTCACTCACCAGAGCTCATCACCTCAAGAGCCCGCGAACTAATCCAAACAGTTCCATCGAGAAAGTCTCTCTGTTCTCTCGTACCTATGGAGCTTATTCAATCTCCCTCAGCTTCGGAACTCCACCGCAGAATTTCTCGTTCGTCTTCGATACGGGAAGCAGCCTCGTCTGGTTCCCCTGCACCGCCGGTTATCTCTGTTCCAATTGTTCGTTTCCGAACTCCGATACTAAGACGATTGCGAAATTTATCCCCAAATTATCTTCCTCTGCGAAGATCGTCGGTTGCCGAAATCGAAAATGTGCTTGGATTTTTGGCCCTAATTTGAAGTCCAGATGTAGAAATTGTAACCCTAAATCTCGAAGGTGTTTCGATTCTTGTCCTGGTTATGGAATTCAGTACGGCTCCGGCGCAACCGCTGGATTTCTCCTCTCTGAAACTCTCGATTTCCCCGAGAAACGAGTGACTGATTTTCTCGTCGGTTGTTCCGTCTTGTCCGTTCATCTACCCGCCGGCATTGCCGGATTCGGCCGCGGCCCTGAATCGTTGCCCTCGCAAATGAGACTGAAACGATTCTCCTATTGCCTGGTTTCTCGCCGGTTCGACGACTCGCCGGTGAGTAGCCCTCTCGTACTGGACTCCGGTTCGGAATCCGGCCAATCGAAGACGAACAGTCTCATCTACGCACCGTTCCGAGAGAATCCGTCGGGATCCAATGCCGCATTTCGAGAATTCTATTACCTTTCTCTTCGGAGAATCCTCATCGTTGGAAAGCCAGTGAAGTTTCCGTACAAGTATCTCGTGCCGGACTCCGCCGGAAACGGCGGTGCGATCATCGATTCCGGTTCGACGTTTACGTTTCTGGATAAGCCGATTTTCGAAGCCGTAGCGGAGGAGTTGGAGAAGCAACTGGTGAAATATCCTCGAGCAACCGGCGTTGAAGCTCAGTCCGGTTTGAGGCCGTGCTTCGATATATCCAAGGAGGAGAAGGTGGAGTTTCCGGAACTGGTTTTGAAGTTTAAAGGCGGAGCGAAGCTGGCTTTGCCGCCGGCGAATTACTTGGCGTTGGTGACGGATTCCGGCGTGGTTTGCTTGACGATGATGACGGACGAGGCCGGAAGCGGCGGCGGTGGCGGTGGGCCGGCGATTATAATTGGGGCGTTTCAGCAGCAGAATGTTTTGGTGGAGTACGATTTGGCGAAGGAGAGAATCGGATTTCGAAAGCAGAGATGCACGTGA

mRNA sequence

ATGGCATTTATGTTGGTAGAAAACGTGGCATGCAGTGTGAACTCGGTGGATTGCAGAGGCCACCAAACTTGTCAATTTACTCTGCTACTGCTTCCATTAACGACTCATCCAAGCTTTTCCAACCAAAACCTCCATGGAGTTTCTCCCCATTCCCTTTCTCTTTTCCATCTTTCTCCTTCTTTCCACTTCATCTTCTTCCTCCAGCTCCCCATCACACTCCCCCTCACCACCTTCCCTTCACTTCCACTTACAGATCCATGGCAGAACATCAATTACCTCGTCTCTGCTTCACTCACCAGAGCTCATCACCTCAAGAGCCCGCGAACTAATCCAAACAGTTCCATCGAGAAAGTCTCTCTGTTCTCTCGTACCTATGGAGCTTATTCAATCTCCCTCAGCTTCGGAACTCCACCGCAGAATTTCTCGTTCGTCTTCGATACGGGAAGCAGCCTCGTCTGGTTCCCCTGCACCGCCGGTTATCTCTGTTCCAATTGTTCGTTTCCGAACTCCGATACTAAGACGATTGCGAAATTTATCCCCAAATTATCTTCCTCTGCGAAGATCGTCGGTTGCCGAAATCGAAAATGTGCTTGGATTTTTGGCCCTAATTTGAAGTCCAGATGTAGAAATTGTAACCCTAAATCTCGAAGGTGTTTCGATTCTTGTCCTGGTTATGGAATTCAGTACGGCTCCGGCGCAACCGCTGGATTTCTCCTCTCTGAAACTCTCGATTTCCCCGAGAAACGAGTGACTGATTTTCTCGTCGGTTGTTCCGTCTTGTCCGTTCATCTACCCGCCGGCATTGCCGGATTCGGCCGCGGCCCTGAATCGTTGCCCTCGCAAATGAGACTGAAACGATTCTCCTATTGCCTGGTTTCTCGCCGGTTCGACGACTCGCCGGTGAGTAGCCCTCTCGTACTGGACTCCGGTTCGGAATCCGGCCAATCGAAGACGAACAGTCTCATCTACGCACCGTTCCGAGAGAATCCGTCGGGATCCAATGCCGCATTTCGAGAATTCTATTACCTTTCTCTTCGGAGAATCCTCATCGTTGGAAAGCCAGTGAAGTTTCCGTACAAGTATCTCGTGCCGGACTCCGCCGGAAACGGCGGTGCGATCATCGATTCCGGTTCGACGTTTACGTTTCTGGATAAGCCGATTTTCGAAGCCGTAGCGGAGGAGTTGGAGAAGCAACTGGTGAAATATCCTCGAGCAACCGGCGTTGAAGCTCAGTCCGGTTTGAGGCCGTGCTTCGATATATCCAAGGAGGAGAAGGTGGAGTTTCCGGAACTGGTTTTGAAGTTTAAAGGCGGAGCGAAGCTGGCTTTGCCGCCGGCGAATTACTTGGCGTTGGTGACGGATTCCGGCGTGGTTTGCTTGACGATGATGACGGACGAGGCCGGAAGCGGCGGCGGTGGCGGTGGGCCGGCGATTATAATTGGGGCGTTTCAGCAGCAGAATGTTTTGGTGGAGTACGATTTGGCGAAGGAGAGAATCGGATTTCGAAAGCAGAGATGCACGTGA

Coding sequence (CDS)

ATGGCATTTATGTTGGTAGAAAACGTGGCATGCAGTGTGAACTCGGTGGATTGCAGAGGCCACCAAACTTGTCAATTTACTCTGCTACTGCTTCCATTAACGACTCATCCAAGCTTTTCCAACCAAAACCTCCATGGAGTTTCTCCCCATTCCCTTTCTCTTTTCCATCTTTCTCCTTCTTTCCACTTCATCTTCTTCCTCCAGCTCCCCATCACACTCCCCCTCACCACCTTCCCTTCACTTCCACTTACAGATCCATGGCAGAACATCAATTACCTCGTCTCTGCTTCACTCACCAGAGCTCATCACCTCAAGAGCCCGCGAACTAATCCAAACAGTTCCATCGAGAAAGTCTCTCTGTTCTCTCGTACCTATGGAGCTTATTCAATCTCCCTCAGCTTCGGAACTCCACCGCAGAATTTCTCGTTCGTCTTCGATACGGGAAGCAGCCTCGTCTGGTTCCCCTGCACCGCCGGTTATCTCTGTTCCAATTGTTCGTTTCCGAACTCCGATACTAAGACGATTGCGAAATTTATCCCCAAATTATCTTCCTCTGCGAAGATCGTCGGTTGCCGAAATCGAAAATGTGCTTGGATTTTTGGCCCTAATTTGAAGTCCAGATGTAGAAATTGTAACCCTAAATCTCGAAGGTGTTTCGATTCTTGTCCTGGTTATGGAATTCAGTACGGCTCCGGCGCAACCGCTGGATTTCTCCTCTCTGAAACTCTCGATTTCCCCGAGAAACGAGTGACTGATTTTCTCGTCGGTTGTTCCGTCTTGTCCGTTCATCTACCCGCCGGCATTGCCGGATTCGGCCGCGGCCCTGAATCGTTGCCCTCGCAAATGAGACTGAAACGATTCTCCTATTGCCTGGTTTCTCGCCGGTTCGACGACTCGCCGGTGAGTAGCCCTCTCGTACTGGACTCCGGTTCGGAATCCGGCCAATCGAAGACGAACAGTCTCATCTACGCACCGTTCCGAGAGAATCCGTCGGGATCCAATGCCGCATTTCGAGAATTCTATTACCTTTCTCTTCGGAGAATCCTCATCGTTGGAAAGCCAGTGAAGTTTCCGTACAAGTATCTCGTGCCGGACTCCGCCGGAAACGGCGGTGCGATCATCGATTCCGGTTCGACGTTTACGTTTCTGGATAAGCCGATTTTCGAAGCCGTAGCGGAGGAGTTGGAGAAGCAACTGGTGAAATATCCTCGAGCAACCGGCGTTGAAGCTCAGTCCGGTTTGAGGCCGTGCTTCGATATATCCAAGGAGGAGAAGGTGGAGTTTCCGGAACTGGTTTTGAAGTTTAAAGGCGGAGCGAAGCTGGCTTTGCCGCCGGCGAATTACTTGGCGTTGGTGACGGATTCCGGCGTGGTTTGCTTGACGATGATGACGGACGAGGCCGGAAGCGGCGGCGGTGGCGGTGGGCCGGCGATTATAATTGGGGCGTTTCAGCAGCAGAATGTTTTGGTGGAGTACGATTTGGCGAAGGAGAGAATCGGATTTCGAAAGCAGAGATGCACGTGA

Protein sequence

MAFMLVENVACSVNSVDCRGHQTCQFTLLLLPLTTHPSFSNQNLHGVSPHSLSLFHLSPSFHFIFFLQLPITLPLTTFPSLPLTDPWQNINYLVSASLTRAHHLKSPRTNPNSSIEKVSLFSRTYGAYSISLSFGTPPQNFSFVFDTGSSLVWFPCTAGYLCSNCSFPNSDTKTIAKFIPKLSSSAKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPGYGIQYGSGATAGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRLKRFSYCLVSRRFDDSPVSSPLVLDSGSESGQSKTNSLIYAPFRENPSGSNAAFREFYYLSLRRILIVGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRATGVEAQSGLRPCFDISKEEKVEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMMTDEAGSGGGGGGPAIIIGAFQQQNVLVEYDLAKERIGFRKQRCT

Homology

[Close]

BLAST of Lag0012806 vs. NCBI nr
Match: XP_022979057.1 (probable aspartyl protease At4g16563 [Cucurbita maxima])

HSP 1 Score: 765.0 bits (1974), Expect = 4.1e-217
Identity = 372/439 (84.74%), Postives = 402/439 (91.57%), Query Frame = 0


Query: 69  LPITLPLTTFPSLPLTDPWQNINYLVSASLTRAHHLKSPRTNPNSSIEKVSLFSRTYGAY 128
           + +TLPLT FPSLPLT PW+NI +LVSASL RA HLK+P+T  N+SI+ V+LF R+YGAY
Sbjct: 25  ITVTLPLTAFPSLPLTHPWKNIKHLVSASLARAQHLKTPKTKSNTSIQNVALFPRSYGAY 84

Query: 129 SISLSFGTPPQNFSFVFDTGSSLVWFPCTAGYLCSNCSFPNSDTKTIAKFIPKLSSSAKI 188
           SISL+FGTPPQ+ S VFDTGSSLVWFPCTAGY CSNCSFPN D  TI KFIPKLSSSA+I
Sbjct: 85  SISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCSNCSFPNVDAATIPKFIPKLSSSARI 144

Query: 189 VGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPGYGIQYGSGATAGFLLSETLDFPEK 248
           +GCRNRKC+WIFGPNLKS CR+C+P+SR+C D+CPGYGIQYGSGATAGFLLSETLDFPEK
Sbjct: 145 IGCRNRKCSWIFGPNLKSSCRSCSPRSRKCSDTCPGYGIQYGSGATAGFLLSETLDFPEK 204

Query: 249 RVTDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRLKRFSYCLVSRRFDDSPVSSPLVLD 308
           RV DFLVGCSVLSVH PAGIAGFGRGPESLPSQM LKRFS+CLV R+FDDSPVSSPLVLD
Sbjct: 205 RVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMGLKRFSHCLVPRQFDDSPVSSPLVLD 264

Query: 309 SGSESGQSKTNSLIYAPFRENPSGSNAAFREFYYLSLRRILIVGKPVKFPYKYLVPDSAG 368
           S  ESG SKTNSLIYAPFRENPSGSNAAFRE+YYL+LRRILI  KPVKFPYKYLVP+SAG
Sbjct: 265 SSPESGDSKTNSLIYAPFRENPSGSNAAFREYYYLTLRRILIGRKPVKFPYKYLVPNSAG 324

Query: 369 NGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRATGVEAQSGLRPCFDISKEEKVEF 428
           NGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRA GVEA+SGLRPCFDISKEE VEF
Sbjct: 325 NGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRAKGVEAESGLRPCFDISKEESVEF 384

Query: 429 PELVLKFKGGAKLALPPANYLALVTDSGVVCLTMMTDEAGSGGGGGGPAIIIGAFQQQNV 488
           PEL+LKFKGGA LALPPANYLALVTD+GVVCLTM+TD     GGGGGPAII GAFQQQNV
Sbjct: 385 PELILKFKGGATLALPPANYLALVTDTGVVCLTMITD-VNFLGGGGGPAIIFGAFQQQNV 444

Query: 489 LVEYDLAKERIGFRKQRCT 508
           LV+YDLAKERIGFRKQRCT
Sbjct: 445 LVQYDLAKERIGFRKQRCT 462

[Close]

BLAST of Lag0012806 vs. NCBI nr
Match: XP_023543736.1 (probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 763.8 bits (1971), Expect = 9.1e-217
Identity = 371/437 (84.90%), Postives = 401/437 (91.76%), Query Frame = 0


Query: 71  ITLPLTTFPSLPLTDPWQNINYLVSASLTRAHHLKSPRTNPNSSIEKVSLFSRTYGAYSI 130
           +TLPLT FPSLP T PW+NI +LVSASLTRA HLK+PR   N+SI+ V+LF R+YGAYSI
Sbjct: 27  VTLPLTVFPSLPFTHPWKNIKHLVSASLTRAQHLKTPRIKSNTSIQNVALFPRSYGAYSI 86

Query: 131 SLSFGTPPQNFSFVFDTGSSLVWFPCTAGYLCSNCSFPNSDTKTIAKFIPKLSSSAKIVG 190
           SL+FGTPPQ+ S VFDTGSSLVWFPCTAGY CSNCSFPN D  TI KFIPKLSSSAKI+G
Sbjct: 87  SLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCSNCSFPNVDAATIPKFIPKLSSSAKIIG 146

Query: 191 CRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPGYGIQYGSGATAGFLLSETLDFPEKRV 250
           CRNRKC+WIFGPNLKS CR+C+P+SR+C D+CPGYGIQYGSGATAGFLLSETLDFPEKRV
Sbjct: 147 CRNRKCSWIFGPNLKSLCRSCSPRSRKCSDTCPGYGIQYGSGATAGFLLSETLDFPEKRV 206

Query: 251 TDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRLKRFSYCLVSRRFDDSPVSSPLVLDSG 310
            DFLVGCSV+SVH PAGIAGFGRGPESLPSQM LKRFS+CLV R+FDDSPVSSPLVLDS 
Sbjct: 207 PDFLVGCSVVSVHQPAGIAGFGRGPESLPSQMGLKRFSHCLVPRQFDDSPVSSPLVLDSS 266

Query: 311 SESGQSKTNSLIYAPFRENPSGSNAAFREFYYLSLRRILIVGKPVKFPYKYLVPDSAGNG 370
           SESG+SK NSLIYAPFRENPSGSNAAFRE+YYL+LRRILI  KPVKFPYKYLVP+SAGNG
Sbjct: 267 SESGESKNNSLIYAPFRENPSGSNAAFREYYYLTLRRILIGRKPVKFPYKYLVPNSAGNG 326

Query: 371 GAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRATGVEAQSGLRPCFDISKEEKVEFPE 430
           GAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRA GVEA+SGLRPCFDISKEE VEFPE
Sbjct: 327 GAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRAKGVEAESGLRPCFDISKEESVEFPE 386

Query: 431 LVLKFKGGAKLALPPANYLALVTDSGVVCLTMMTDEAGSGGGGGGPAIIIGAFQQQNVLV 490
           L+LKFKGGA LALPPANYLALVTD+GVVCLTM+TD     GGGGGPAII GAFQQQNVLV
Sbjct: 387 LILKFKGGATLALPPANYLALVTDTGVVCLTMITDVTFL-GGGGGPAIIFGAFQQQNVLV 446

Query: 491 EYDLAKERIGFRKQRCT 508
           +YDLAK+RIGFRKQRCT
Sbjct: 447 QYDLAKDRIGFRKQRCT 462

[Close]

BLAST of Lag0012806 vs. NCBI nr
Match: XP_022925946.1 (probable aspartyl protease At4g16563 [Cucurbita moschata] >KAG6604319.1 Aspartic proteinase nepenthesin-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 758.8 bits (1958), Expect = 2.9e-215
Identity = 368/437 (84.21%), Postives = 399/437 (91.30%), Query Frame = 0


Query: 71  ITLPLTTFPSLPLTDPWQNINYLVSASLTRAHHLKSPRTNPNSSIEKVSLFSRTYGAYSI 130
           +TLPLT FPSLP   PW+NI +LVSASLTRA HLK+PRT  N+SI+ V+LF R+YGAYSI
Sbjct: 27  VTLPLTVFPSLPFAHPWKNIKHLVSASLTRAQHLKTPRTKSNTSIQNVALFPRSYGAYSI 86

Query: 131 SLSFGTPPQNFSFVFDTGSSLVWFPCTAGYLCSNCSFPNSDTKTIAKFIPKLSSSAKIVG 190
           SL+FGTPPQ+ S VFDTGSSLVWFPCTAGY CSNCSFPN D  TI KFIPKLSSSAKI+G
Sbjct: 87  SLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCSNCSFPNVDAATIPKFIPKLSSSAKIIG 146

Query: 191 CRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPGYGIQYGSGATAGFLLSETLDFPEKRV 250
           CRNRKC+WIFGPNLK+ CR+C+P+SR+C D+CPGYGIQYGSGATAGFLLSETLDFPEKRV
Sbjct: 147 CRNRKCSWIFGPNLKTLCRSCSPRSRKCSDTCPGYGIQYGSGATAGFLLSETLDFPEKRV 206

Query: 251 TDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRLKRFSYCLVSRRFDDSPVSSPLVLDSG 310
            DFLVGCSV+SVH PAGIAGFGRGPESLPSQM LKRFS+CLV R+FDDSPVSSPLVLDS 
Sbjct: 207 PDFLVGCSVVSVHQPAGIAGFGRGPESLPSQMGLKRFSHCLVPRQFDDSPVSSPLVLDSS 266

Query: 311 SESGQSKTNSLIYAPFRENPSGSNAAFREFYYLSLRRILIVGKPVKFPYKYLVPDSAGNG 370
           SESG+SK NSLIYAPFRENPSGSNAAFRE+YYL+LRRILI  KPVKFPYKYLVP+SAGNG
Sbjct: 267 SESGESKNNSLIYAPFRENPSGSNAAFREYYYLTLRRILIGRKPVKFPYKYLVPNSAGNG 326

Query: 371 GAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRATGVEAQSGLRPCFDISKEEKVEFPE 430
           GAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRA GVEA+SGLRPCFDISKEE VEFPE
Sbjct: 327 GAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRAKGVEAESGLRPCFDISKEESVEFPE 386

Query: 431 LVLKFKGGAKLALPPANYLALVTDSGVVCLTMMTDEAGSGGGGGGPAIIIGAFQQQNVLV 490
           L+LKFKGGA LALPP+NYLALV D+ VVCLTM+TD     GGGGGPAII GAFQQQNVLV
Sbjct: 387 LILKFKGGATLALPPSNYLALVADTSVVCLTMITDVTFL-GGGGGPAIIFGAFQQQNVLV 446

Query: 491 EYDLAKERIGFRKQRCT 508
           +YDLAKERIGFRKQRCT
Sbjct: 447 QYDLAKERIGFRKQRCT 462

[Close]

BLAST of Lag0012806 vs. NCBI nr
Match: KAG7034471.1 (Aspartic proteinase nepenthesin-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 757.7 bits (1955), Expect = 6.5e-215
Identity = 368/437 (84.21%), Postives = 398/437 (91.08%), Query Frame = 0


Query: 71  ITLPLTTFPSLPLTDPWQNINYLVSASLTRAHHLKSPRTNPNSSIEKVSLFSRTYGAYSI 130
           +TLPLT FPSLP   PW+NI +LVSASLTRA HLK PRT  N+SI+ V+LF R+YGAYSI
Sbjct: 27  VTLPLTVFPSLPFAHPWKNIKHLVSASLTRAQHLKIPRTKSNTSIQNVALFPRSYGAYSI 86

Query: 131 SLSFGTPPQNFSFVFDTGSSLVWFPCTAGYLCSNCSFPNSDTKTIAKFIPKLSSSAKIVG 190
           SL+FGTPPQ+ S VFDTGSSLVWFPCTAGY CSNCSFPN D  TI KFIPKLSSSAKI+G
Sbjct: 87  SLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCSNCSFPNVDAATIPKFIPKLSSSAKIIG 146

Query: 191 CRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPGYGIQYGSGATAGFLLSETLDFPEKRV 250
           CRNRKC+WIFGPNLK+ CR+C+P+SR+C D+CPGYGIQYGSGATAGFLLSETLDFPEKRV
Sbjct: 147 CRNRKCSWIFGPNLKTLCRSCSPRSRKCSDTCPGYGIQYGSGATAGFLLSETLDFPEKRV 206

Query: 251 TDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRLKRFSYCLVSRRFDDSPVSSPLVLDSG 310
            DFLVGCSV+SVH PAGIAGFGRGPESLPSQM LKRFS+CLV R+FDDSPVSSPLVLDS 
Sbjct: 207 PDFLVGCSVVSVHQPAGIAGFGRGPESLPSQMGLKRFSHCLVPRQFDDSPVSSPLVLDSS 266

Query: 311 SESGQSKTNSLIYAPFRENPSGSNAAFREFYYLSLRRILIVGKPVKFPYKYLVPDSAGNG 370
           SESG+SK NSLIYAPFRENPSGSNAAFRE+YYL+LRRILI  KPVKFPYKYLVP+SAGNG
Sbjct: 267 SESGESKNNSLIYAPFRENPSGSNAAFREYYYLTLRRILIGRKPVKFPYKYLVPNSAGNG 326

Query: 371 GAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRATGVEAQSGLRPCFDISKEEKVEFPE 430
           GAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRA GVEA+SGLRPCFDISKEE VEFPE
Sbjct: 327 GAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRAKGVEAESGLRPCFDISKEESVEFPE 386

Query: 431 LVLKFKGGAKLALPPANYLALVTDSGVVCLTMMTDEAGSGGGGGGPAIIIGAFQQQNVLV 490
           L+LKFKGGA LALPP+NYLALV D+ VVCLTM+TD     GGGGGPAII GAFQQQNVLV
Sbjct: 387 LILKFKGGATLALPPSNYLALVADTSVVCLTMITDVTFL-GGGGGPAIIFGAFQQQNVLV 446

Query: 491 EYDLAKERIGFRKQRCT 508
           +YDLAKERIGFRKQRCT
Sbjct: 447 QYDLAKERIGFRKQRCT 462

[Close]

BLAST of Lag0012806 vs. NCBI nr
Match: XP_038881211.1 (probable aspartyl protease At4g16563 [Benincasa hispida])

HSP 1 Score: 740.7 bits (1911), Expect = 8.2e-210
Identity = 376/457 (82.28%), Postives = 393/457 (86.00%), Query Frame = 0


Query: 61  FHFIFFLQLP---------ITLPLTTFPSLPLT-DPWQNINYLVSASLTRAHHLKSPRTN 120
           F F  FL LP         ITLPLT FPS+PLT DP + INYL+SASL RA HLK+P+T 
Sbjct: 8   FLFSIFLLLPTSSSSSISTITLPLTAFPSIPLTDDPLKIINYLLSASLNRAQHLKNPQT- 67

Query: 121 PNSSIEKVSLFSRTYGAYSISLSFGTPPQNFSFVFDTGSSLVWFPCTAGYLCSNCSFPNS 180
              SI+ VSLFSR+YGAYSI+L+FGTPPQN SFVFDTGSSLVWFPCTAGY CSNCSFPN 
Sbjct: 68  --KSIQNVSLFSRSYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCSNCSFPNV 127

Query: 181 DTKTIAKFIPKLSSSAKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPGYGIQYG 240
           D  TI KF+PKLSSSAKI+GCRN KCAWIFGPNL SRCRNCNPKSR C  SCPGYGI YG
Sbjct: 128 DAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLNSRCRNCNPKSRNCSGSCPGYGILYG 187

Query: 241 SGATAGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRLKRFSYC 300
           SGATAGFLLSETLDFP+K V DFLVGCSV SVH PAGIAGFGR PESLPSQMRLKRFSYC
Sbjct: 188 SGATAGFLLSETLDFPKKGVPDFLVGCSVSSVHQPAGIAGFGRAPESLPSQMRLKRFSYC 247

Query: 301 LVSRRFDDSPVSSPLVLDSGSESGQSKTNSLIYAPFRENPSGSNAAFREFYYLSLRRILI 360
           LVSR FDDSPVSSPLVLDSGSES  SKT S IYAPFRENPSGSNAAFRE+YYLSLRRILI
Sbjct: 248 LVSRGFDDSPVSSPLVLDSGSESDDSKTESFIYAPFRENPSGSNAAFREYYYLSLRRILI 307

Query: 361 VGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRATGVEA 420
            GKPVK PYKYL+PDSAG GGAIIDSGSTFTFLDKPIFEAVA ELEKQLVKYPR   VE 
Sbjct: 308 GGKPVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGELEKQLVKYPRTKSVEV 367

Query: 421 QSGLRPCFDISKEEKVEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMMTDEAGSG 480
           QSGLRPCFDISKE  VEFPELVLKFKGGAKL+LPP NYLALVTD+GVVCLTMMTD  G  
Sbjct: 368 QSGLRPCFDISKEVSVEFPELVLKFKGGAKLSLPPVNYLALVTDAGVVCLTMMTD-VGVV 427

Query: 481 GGGGGPAIIIGAFQQQNVLVEYDLAKERIGFRKQRCT 508
           GGG GPAII GAFQQQNVLVEYDLA+ RIGFRKQRCT
Sbjct: 428 GGGAGPAIIFGAFQQQNVLVEYDLARNRIGFRKQRCT 460

[Close]

BLAST of Lag0012806 vs. ExPASy Swiss-Prot
Match: Q940R4 (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g16563 PE=2 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 1.2e-46
Identity = 159/498 (31.93%), Postives = 230/498 (46.18%), Query Frame = 0


Query: 61  FHF-IFFLQLPITLPLTTFPSLPLTDPWQNINYLVSASLTRAHHLKSPRTNPNSSIEKVS 120
           FHF +  L  P+ L L+   SL  +    +  +L+ +S +R+   +  R +     +++S
Sbjct: 18  FHFSVSSLSTPLLLHLS--HSLSTSKHSSSPLHLLKSSSSRS-SARFRRHHHKQQQQQLS 77

Query: 121 LFSRTYGAYSISLSFGTPPQNFSFVFDTGSSLVWFPCTAGYLCSNCSFPNSDTKTIAKFI 180
           L   +   Y ISLS G+     S   DTGS LVWFPC   + C  C     ++K +    
Sbjct: 78  LPISSGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPCRP-FTCILC-----ESKPLPPSP 137

Query: 181 P-KLSSSAKIVGCRNRKCAWIFGPNLKS-------------RCRNCNPKSRRCFDSCPGY 240
           P  LSSSA  V C +  C+        S                +CN  S      CP +
Sbjct: 138 PSSLSSSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSY----PCPPF 197

Query: 241 GIQYGSGATAGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRL- 300
              YG G+    L S++L  P   V++F  GC+  ++  P G+AGFGRG  SLP+Q+ + 
Sbjct: 198 YYAYGDGSLVAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVH 257

Query: 301 -----KRFSYCLVSRRFDDSPV--SSPLVL-----------------DSGSESGQSKTNS 360
                  FSYCLVS  FD   V   SPL+L                 D G +  + K N 
Sbjct: 258 SPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDE-KKKKNE 317

Query: 361 LIYAPFRENPSGSNAAFREFYYLSLRRILIVGKPVKFPYKYLVPDSAGNGGAIIDSGSTF 420
            ++    ENP         FY +SL+ I I  + +  P      D  G GG ++DSG+TF
Sbjct: 318 FVFTEMLENPK-----HPYFYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTF 377

Query: 421 TFLDKPIFEAVAEELEKQLVK-YPRATGVEAQSGLRPCFDISKEEKVEFPELVLKFKGG- 480
           T L    + +V EE + ++ + + RA  VE  SG+ PC+ +++  KV  P LVL F G  
Sbjct: 378 TMLPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLNQTVKV--PALVLHFAGNR 437

Query: 481 AKLALPPANYLALVTDSG--------VVCLTMMT--DEAGSGGGGGGPAIIIGAFQQQNV 507
           + + LP  NY     D G        + CL +M   DE+   GG G    I+G +QQQ  
Sbjct: 438 SSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGA---ILGNYQQQGF 491

[Close]

BLAST of Lag0012806 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 6.2e-35
Identity = 119/390 (30.51%), Postives = 173/390 (44.36%), Query Frame = 0


Query: 126 GAYSISLSFGTPPQNFSFVFDTGSSLVWFPCTAGYLCSNCSFPNSDTKTIAKFIPKLSSS 185
           G Y ++LS GTP Q FS + DTGS L+W  C     C N S P         F P+ SSS
Sbjct: 93  GEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTP--------IFNPQGSSS 152

Query: 186 AKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPGYGIQYGSGA-TAGFLLSETLD 245
              + C ++ C  +  P               C ++   Y   YG G+ T G + +ETL 
Sbjct: 153 FSTLPCSSQLCQALSSPT--------------CSNNFCQYTYGYGDGSETQGSMGTETLT 212

Query: 246 FPEKRVTDFLVGCSVLSVHL----PAGIAGFGRGPESLPSQMRLKRFSYCLVSRRFDDSP 305
           F    + +   GC   +        AG+ G GRGP SLPSQ+ + +FSYC+       S 
Sbjct: 213 FGSVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTP---IGSS 272

Query: 306 VSSPLVLDS--GSESGQSKTNSLIYAPFRENPSGSNAAFREFYYLSLRRILIVGKPVKF- 365
             S L+L S   S +  S   +LI           ++    FYY++L  + +    +   
Sbjct: 273 TPSNLLLGSLANSVTAGSPNTTLI----------QSSQIPTFYYITLNGLSVGSTRLPID 332

Query: 366 PYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRATGVEAQSGLRPC 425
           P  + +  + G GG IIDSG+T T+     +++V +E   Q +  P   G  + SG   C
Sbjct: 333 PSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQ-INLPVVNG--SSSGFDLC 392

Query: 426 FDI-SKEEKVEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMMTDEAGSGGGGGGP 485
           F   S    ++ P  V+ F GG  L LP  NY  +   +G++CL M +   G        
Sbjct: 393 FQTPSDPSNLQIPTFVMHFDGG-DLELPSENYF-ISPSNGLICLAMGSSSQGMS------ 434

Query: 486 AIIIGAFQQQNVLVEYDLAKERIGFRKQRC 507
             I G  QQQN+LV YD     + F   +C
Sbjct: 453 --IFGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of Lag0012806 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 8.2e-35
Identity = 132/409 (32.27%), Postives = 177/409 (43.28%), Query Frame = 0


Query: 106 SPRTNPNSSIEKVSLFSRTYGAYSISLSFGTPPQNFSFVFDTGSSLVWFPCTAGYLCSNC 165
           +PR    SS   VS  S+  G Y   L  GTP +    V DTGS +VW  C     C + 
Sbjct: 121 APRPGGFSS-SVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQ 180

Query: 166 SFPNSDTKTIAKFIPKLSSSAKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPGY 225
           S P  D        P+ S +   + C +  C        +     CN + + C      Y
Sbjct: 181 SDPIFD--------PRKSKTYATIPCSSPHCR-------RLDSAGCNTRRKTCL-----Y 240

Query: 226 GIQYGSGA-TAGFLLSETLDFPEKRVTDFLVGCSVLSVHL---PAGIAGFGRGPESLPSQ 285
            + YG G+ T G   +ETL F   RV    +GC   +  L    AG+ G G+G  S P Q
Sbjct: 241 QVSYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQ 300

Query: 286 MRLK---RFSYCLVSRRFDDSPVSSPLVLDSGSESGQSKTNSLIYAPFRENPSGSNAAFR 345
              +   +FSYCLV R     P  S +V  + + S  ++   L+          SN    
Sbjct: 301 TGHRFNQKFSYCLVDRSASSKP--SSVVFGNAAVSRIARFTPLL----------SNPKLD 360

Query: 346 EFYYLSLRRILIVGKPVKFPYKYLVP-DSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEK 405
            FYY+ L  I + G  V      L   D  GNGG IIDSG++ T L +P + A+ +    
Sbjct: 361 TFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRV 420

Query: 406 QLVKYPRATGVEAQSGLRPCFDISKEEKVEFPELVLKFKGGAKLALPPANYLALVTDSGV 465
                 RA      S    CFD+S   +V+ P +VL F+ GA ++LP  NYL  V  +G 
Sbjct: 421 GAKTLKRAPDF---SLFDTCFDLSNMNEVKVPTVVLHFR-GADVSLPATNYLIPVDTNGK 480

Query: 466 VCLTMMTDEAGSGGGGGGPAIIIGAFQQQNVLVEYDLAKERIGFRKQRC 507
            C       AG+ GG      IIG  QQQ   V YDLA  R+GF    C
Sbjct: 481 FCFAF----AGTMGG----LSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

[Close]

BLAST of Lag0012806 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 6.9e-34
Identity = 120/394 (30.46%), Postives = 170/394 (43.15%), Query Frame = 0


Query: 118 VSLFSRTYGAYSISLSFGTPPQNFSFVFDTGSSLVWFPCTAGYLCSNCSFPNSDTKTIAK 177
           VS  S+  G Y   +  GTP +    V DTGS + W  C     C++C +  SD      
Sbjct: 152 VSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEP---CADC-YQQSD----PV 211

Query: 178 FIPKLSSSAKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPGYGIQYGSGA-TAG 237
           F P  SS+ K + C   +C+ +      S CR     S +C      Y + YG G+ T G
Sbjct: 212 FNPTSSSTYKSLTCSAPQCSLL----ETSACR-----SNKCL-----YQVSYGDGSFTVG 271

Query: 238 FLLSETLDFPEK-RVTDFLVGCSVLSVHL---PAGIAGFGRGPESLPSQMRLKRFSYCLV 297
            L ++T+ F    ++ +  +GC   +  L    AG+ G G G  S+ +QM+   FSYCLV
Sbjct: 272 ELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATSFSYCLV 331

Query: 298 SRRFDDSPVSSPLVLDSGSESGQSKTNSLIYAPFRENPSGSNAAFREFYYLSLRRILIVG 357
            R   DS  SS L  +S    G   T  L+           N     FYY+ L    + G
Sbjct: 332 DR---DSGKSSSLDFNSVQLGGGDATAPLL----------RNKKIDTFYYVGLSGFSVGG 391

Query: 358 KPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRATGVEAQS 417
           + V  P      D++G+GG I+D G+  T L    + ++ +   K  V   +  G  + S
Sbjct: 392 EKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKK--GSSSIS 451

Query: 418 GLRPCFDISKEEKVEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMMTDEAGSGGG 477
               C+D S    V+ P +   F GG  L LP  NYL  V DSG  C       +     
Sbjct: 452 LFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLS-- 500

Query: 478 GGGPAIIIGAFQQQNVLVEYDLAKERIGFRKQRC 507
                 IIG  QQQ   + YDL+K  IG    +C
Sbjct: 512 ------IIGNVQQQGTRITYDLSKNVIGLSGNKC 500

[Close]

BLAST of Lag0012806 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 4.9e-32
Identity = 123/391 (31.46%), Postives = 177/391 (45.27%), Query Frame = 0


Query: 126 GAYSISLSFGTPPQNFSFVFDTGSSLVWFPCTAGYLCSNCSFPNSDTKTIAKFIPKLSSS 185
           G Y ++++ GTP  +FS + DTGS L+W  C     C+ C      ++    F P+ SSS
Sbjct: 94  GEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEP---CTQCF-----SQPTPIFNPQDSSS 153

Query: 186 AKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPGYGIQYGSGATA-GFLLSETLD 245
              + C ++ C               +  S  C ++   Y   YG G+T  G++ +ET  
Sbjct: 154 FSTLPCESQYCQ--------------DLPSETCNNNECQYTYGYGDGSTTQGYMATETFT 213

Query: 246 FPEKRVTDFLVGCSV----LSVHLPAGIAGFGRGPESLPSQMRLKRFSYCLVSRRFDDSP 305
           F    V +   GC            AG+ G G GP SLPSQ+ + +FSYC+ S       
Sbjct: 214 FETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTS-----YG 273

Query: 306 VSSPLVLDSGSESG----QSKTNSLIYAPFRENPSGSNAAFREFYYLSLRRILIVGKPVK 365
            SSP  L  GS +      S + +LI++    NP+        +YY++L+ I + G  + 
Sbjct: 274 SSSPSTLALGSAASGVPEGSPSTTLIHSSL--NPT--------YYYITLQGITVGGDNLG 333

Query: 366 FPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRATGVEAQSGLRP 425
            P         G GG IIDSG+T T+L +  + AVA+    Q +  P  T  E+ SGL  
Sbjct: 334 IPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQ-INLP--TVDESSSGLST 393

Query: 426 CF-DISKEEKVEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMMTDEAGSGGGGGG 485
           CF   S    V+ PE+ ++F GG  L L   N L +    GV+CL M     GS    G 
Sbjct: 394 CFQQPSDGSTVQVPEISMQFDGGV-LNLGEQNIL-ISPAEGVICLAM-----GSSSQLG- 435

Query: 486 PAIIIGAFQQQNVLVEYDLAKERIGFRKQRC 507
              I G  QQQ   V YDL    + F   +C
Sbjct: 454 -ISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of Lag0012806 vs. ExPASy TrEMBL
Match: A0A6J1IMR7 (probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111478813 PE=3 SV=1)

HSP 1 Score: 765.0 bits (1974), Expect = 2.0e-217
Identity = 372/439 (84.74%), Postives = 402/439 (91.57%), Query Frame = 0


Query: 69  LPITLPLTTFPSLPLTDPWQNINYLVSASLTRAHHLKSPRTNPNSSIEKVSLFSRTYGAY 128
           + +TLPLT FPSLPLT PW+NI +LVSASL RA HLK+P+T  N+SI+ V+LF R+YGAY
Sbjct: 25  ITVTLPLTAFPSLPLTHPWKNIKHLVSASLARAQHLKTPKTKSNTSIQNVALFPRSYGAY 84

Query: 129 SISLSFGTPPQNFSFVFDTGSSLVWFPCTAGYLCSNCSFPNSDTKTIAKFIPKLSSSAKI 188
           SISL+FGTPPQ+ S VFDTGSSLVWFPCTAGY CSNCSFPN D  TI KFIPKLSSSA+I
Sbjct: 85  SISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCSNCSFPNVDAATIPKFIPKLSSSARI 144

Query: 189 VGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPGYGIQYGSGATAGFLLSETLDFPEK 248
           +GCRNRKC+WIFGPNLKS CR+C+P+SR+C D+CPGYGIQYGSGATAGFLLSETLDFPEK
Sbjct: 145 IGCRNRKCSWIFGPNLKSSCRSCSPRSRKCSDTCPGYGIQYGSGATAGFLLSETLDFPEK 204

Query: 249 RVTDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRLKRFSYCLVSRRFDDSPVSSPLVLD 308
           RV DFLVGCSVLSVH PAGIAGFGRGPESLPSQM LKRFS+CLV R+FDDSPVSSPLVLD
Sbjct: 205 RVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMGLKRFSHCLVPRQFDDSPVSSPLVLD 264

Query: 309 SGSESGQSKTNSLIYAPFRENPSGSNAAFREFYYLSLRRILIVGKPVKFPYKYLVPDSAG 368
           S  ESG SKTNSLIYAPFRENPSGSNAAFRE+YYL+LRRILI  KPVKFPYKYLVP+SAG
Sbjct: 265 SSPESGDSKTNSLIYAPFRENPSGSNAAFREYYYLTLRRILIGRKPVKFPYKYLVPNSAG 324

Query: 369 NGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRATGVEAQSGLRPCFDISKEEKVEF 428
           NGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRA GVEA+SGLRPCFDISKEE VEF
Sbjct: 325 NGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRAKGVEAESGLRPCFDISKEESVEF 384

Query: 429 PELVLKFKGGAKLALPPANYLALVTDSGVVCLTMMTDEAGSGGGGGGPAIIIGAFQQQNV 488
           PEL+LKFKGGA LALPPANYLALVTD+GVVCLTM+TD     GGGGGPAII GAFQQQNV
Sbjct: 385 PELILKFKGGATLALPPANYLALVTDTGVVCLTMITD-VNFLGGGGGPAIIFGAFQQQNV 444

Query: 489 LVEYDLAKERIGFRKQRCT 508
           LV+YDLAKERIGFRKQRCT
Sbjct: 445 LVQYDLAKERIGFRKQRCT 462

[Close]

BLAST of Lag0012806 vs. ExPASy TrEMBL
Match: A0A6J1EDJ0 (probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC111433208 PE=3 SV=1)

HSP 1 Score: 758.8 bits (1958), Expect = 1.4e-215
Identity = 368/437 (84.21%), Postives = 399/437 (91.30%), Query Frame = 0


Query: 71  ITLPLTTFPSLPLTDPWQNINYLVSASLTRAHHLKSPRTNPNSSIEKVSLFSRTYGAYSI 130
           +TLPLT FPSLP   PW+NI +LVSASLTRA HLK+PRT  N+SI+ V+LF R+YGAYSI
Sbjct: 27  VTLPLTVFPSLPFAHPWKNIKHLVSASLTRAQHLKTPRTKSNTSIQNVALFPRSYGAYSI 86

Query: 131 SLSFGTPPQNFSFVFDTGSSLVWFPCTAGYLCSNCSFPNSDTKTIAKFIPKLSSSAKIVG 190
           SL+FGTPPQ+ S VFDTGSSLVWFPCTAGY CSNCSFPN D  TI KFIPKLSSSAKI+G
Sbjct: 87  SLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCSNCSFPNVDAATIPKFIPKLSSSAKIIG 146

Query: 191 CRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPGYGIQYGSGATAGFLLSETLDFPEKRV 250
           CRNRKC+WIFGPNLK+ CR+C+P+SR+C D+CPGYGIQYGSGATAGFLLSETLDFPEKRV
Sbjct: 147 CRNRKCSWIFGPNLKTLCRSCSPRSRKCSDTCPGYGIQYGSGATAGFLLSETLDFPEKRV 206

Query: 251 TDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRLKRFSYCLVSRRFDDSPVSSPLVLDSG 310
            DFLVGCSV+SVH PAGIAGFGRGPESLPSQM LKRFS+CLV R+FDDSPVSSPLVLDS 
Sbjct: 207 PDFLVGCSVVSVHQPAGIAGFGRGPESLPSQMGLKRFSHCLVPRQFDDSPVSSPLVLDSS 266

Query: 311 SESGQSKTNSLIYAPFRENPSGSNAAFREFYYLSLRRILIVGKPVKFPYKYLVPDSAGNG 370
           SESG+SK NSLIYAPFRENPSGSNAAFRE+YYL+LRRILI  KPVKFPYKYLVP+SAGNG
Sbjct: 267 SESGESKNNSLIYAPFRENPSGSNAAFREYYYLTLRRILIGRKPVKFPYKYLVPNSAGNG 326

Query: 371 GAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRATGVEAQSGLRPCFDISKEEKVEFPE 430
           GAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRA GVEA+SGLRPCFDISKEE VEFPE
Sbjct: 327 GAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRAKGVEAESGLRPCFDISKEESVEFPE 386

Query: 431 LVLKFKGGAKLALPPANYLALVTDSGVVCLTMMTDEAGSGGGGGGPAIIIGAFQQQNVLV 490
           L+LKFKGGA LALPP+NYLALV D+ VVCLTM+TD     GGGGGPAII GAFQQQNVLV
Sbjct: 387 LILKFKGGATLALPPSNYLALVADTSVVCLTMITDVTFL-GGGGGPAIIFGAFQQQNVLV 446

Query: 491 EYDLAKERIGFRKQRCT 508
           +YDLAKERIGFRKQRCT
Sbjct: 447 QYDLAKERIGFRKQRCT 462

[Close]

BLAST of Lag0012806 vs. ExPASy TrEMBL
Match: A0A0A0KHK2 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G454470 PE=3 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 5.4e-207
Identity = 362/455 (79.56%), Postives = 396/455 (87.03%), Query Frame = 0


Query: 61  FHFIFFLQLPIT-------LPLTTFPSLPLTDPWQNINYLVSASLTRAHHLKSPRTNPNS 120
           F F  FL LP +       LPLTTFPS+  TDP++ IN L+SASL RA HLK+P++  N+
Sbjct: 8   FLFSIFLLLPTSSSSSTTVLPLTTFPSVSFTDPFKTINLLLSASLNRAQHLKTPQSKSNT 67

Query: 121 SIEKVSLFSRTYGAYSISLSFGTPPQNFSFVFDTGSSLVWFPCTAGYLCSNCSFPNSDTK 180
           SI+ VSLF R+YGAYS+SL+FGTPPQN SF+FDTGSSLVWFPCTAGY CS CSFP  D  
Sbjct: 68  SIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSRCSFPYVDPA 127

Query: 181 TIAKFIPKLSSSAKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPGYGIQYGSGA 240
           TI+KF+PKLSSS K+VGCRN KCAWIFGPNLKSRCRNCN KSR+C DSCPGYG+QYGSGA
Sbjct: 128 TISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPGYGLQYGSGA 187

Query: 241 TAGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRLKRFSYCLVS 300
           TAG LLSETLD   KRV DFLVGCSV+SVH PAGIAGFGRGPESLPSQMRLKRFS+CLVS
Sbjct: 188 TAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKRFSHCLVS 247

Query: 301 RRFDDSPVSSPLVLDSGSESGQSKTNSLIYAPFRENPSGSNAAFREFYYLSLRRILIVGK 360
           R FDDSPVSSPLVLDSGSES +SKT S IYAPFRENPS SNAAFRE+YYLSLRRILI GK
Sbjct: 248 RGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLSLRRILIGGK 307

Query: 361 PVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRATGVEAQSG 420
           PVKFPYKYLVPDS GNGGAIIDSGSTFTFLDKPIFEA+A+ELEKQLVKYPRA  VEAQSG
Sbjct: 308 PVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPRAKDVEAQSG 367

Query: 421 LRPCFDISKEEK-VEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMMTDEAGSGGG 480
           LRPCF+I KEE+  EFP++VLKFKGG KL+L   NYLA+VTD GVVCLTMMTDEA   GG
Sbjct: 368 LRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTMMTDEA-VVGG 427

Query: 481 GGGPAIIIGAFQQQNVLVEYDLAKERIGFRKQRCT 508
           GGGPAII+GAFQQQNVLVEYDLAK+RIGFRKQ+CT
Sbjct: 428 GGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKCT 461

[Close]

BLAST of Lag0012806 vs. ExPASy TrEMBL
Match: A0A5A7SGF9 (Aspartic proteinase nepenthesin-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G00670 PE=3 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 7.3e-204
Identity = 355/454 (78.19%), Postives = 393/454 (86.56%), Query Frame = 0


Query: 61  FHFIFFLQLP------ITLPLTTFPSLPLTDPWQNINYLVSASLTRAHHLKSPRTNPNSS 120
           F F  FL LP      ITLPL TFPS+P TDP + IN+L+SASL+RA HLKSP++  N+S
Sbjct: 8   FLFSIFLLLPTSSSSSITLPLATFPSIPFTDPLKTINHLLSASLSRAQHLKSPQSKSNTS 67

Query: 121 IEKVSLFSRTYGAYSISLSFGTPPQNFSFVFDTGSSLVWFPCTAGYLCSNCSFPNSDTKT 180
            E VSLF R+YGAY++SL+FGTPPQN SF+FDTGSSLVWFPCTAGY C++CSFP+ D  T
Sbjct: 68  TENVSLFPRSYGAYAVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCAHCSFPHVDPAT 127

Query: 181 IAKFIPKLSSSAKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPGYGIQYGSGAT 240
           I+KF+PKLSSS KIVGCRN KCAWIFGPNLKSRCRNCNPKSR+C DSCPGYGIQYGSGAT
Sbjct: 128 ISKFVPKLSSSVKIVGCRNPKCAWIFGPNLKSRCRNCNPKSRKCSDSCPGYGIQYGSGAT 187

Query: 241 AGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRLKRFSYCLVSR 300
           AG LLSETLD   KRV DFLVGCSV+SVH PAGIAGFGRGPESLPSQMRLKRFS+CL+ R
Sbjct: 188 AGILLSETLDLQNKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKRFSHCLLPR 247

Query: 301 RFDDSPVSSPLVLDSGSESGQSKTNSLIYAPFRENPSGSNAAFREFYYLSLRRILIVGKP 360
            FDDSPVSSPLVLDSG ES +SKT S IYAPF+ENPS SN AFRE+YYLSLRRILI GKP
Sbjct: 248 GFDDSPVSSPLVLDSGPESDESKTKSFIYAPFQENPSRSNTAFREYYYLSLRRILIGGKP 307

Query: 361 VKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRATGVEAQSGL 420
           VKFPYKYLVPDS G GGAIIDSGSTFTFLDKPIFEA+A ELEKQLVKYPRA  +EA++GL
Sbjct: 308 VKFPYKYLVPDSTGKGGAIIDSGSTFTFLDKPIFEAIAGELEKQLVKYPRAKDIEAKTGL 367

Query: 421 RPCFDISKEEK-VEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMMTDEAGSGGGG 480
           RPCF+ISKEE+  EFPE+ LKFKGG KL+LPP NYL +VTD+ VVCLTMMT+ A   G G
Sbjct: 368 RPCFNISKEEESAEFPEVALKFKGGGKLSLPPENYLVMVTDANVVCLTMMTN-AEVVGVG 427

Query: 481 GGPAIIIGAFQQQNVLVEYDLAKERIGFRKQRCT 508
           GGPAII GAFQQQNVLVEYDLAK+RIGFRKQ+CT
Sbjct: 428 GGPAIIFGAFQQQNVLVEYDLAKQRIGFRKQKCT 460

[Close]

BLAST of Lag0012806 vs. ExPASy TrEMBL
Match: A0A1S3CHV2 (aspartic proteinase nepenthesin-2-like OS=Cucumis melo OX=3656 GN=LOC103500932 PE=3 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 7.3e-204
Identity = 355/454 (78.19%), Postives = 393/454 (86.56%), Query Frame = 0


Query: 61  FHFIFFLQLP------ITLPLTTFPSLPLTDPWQNINYLVSASLTRAHHLKSPRTNPNSS 120
           F F  FL LP      ITLPL TFPS+P TDP + IN+L+SASL+RA HLKSP++  N+S
Sbjct: 8   FLFSIFLLLPTSSSSSITLPLATFPSIPFTDPLKTINHLLSASLSRAQHLKSPQSKSNTS 67

Query: 121 IEKVSLFSRTYGAYSISLSFGTPPQNFSFVFDTGSSLVWFPCTAGYLCSNCSFPNSDTKT 180
            E VSLF R+YGAY++SL+FGTPPQN SF+FDTGSSLVWFPCTAGY C++CSFP+ D  T
Sbjct: 68  TENVSLFPRSYGAYAVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCAHCSFPHVDPAT 127

Query: 181 IAKFIPKLSSSAKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPGYGIQYGSGAT 240
           I+KF+PKLSSS KIVGCRN KCAWIFGPNLKSRCRNCNPKSR+C DSCPGYGIQYGSGAT
Sbjct: 128 ISKFVPKLSSSVKIVGCRNPKCAWIFGPNLKSRCRNCNPKSRKCSDSCPGYGIQYGSGAT 187

Query: 241 AGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRLKRFSYCLVSR 300
           AG LLSETLD   KRV DFLVGCSV+SVH PAGIAGFGRGPESLPSQMRLKRFS+CL+ R
Sbjct: 188 AGILLSETLDLQNKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRLKRFSHCLLPR 247

Query: 301 RFDDSPVSSPLVLDSGSESGQSKTNSLIYAPFRENPSGSNAAFREFYYLSLRRILIVGKP 360
            FDDSPVSSPLVLDSG ES +SKT S IYAPF+ENPS SN AFRE+YYLSLRRILI GKP
Sbjct: 248 GFDDSPVSSPLVLDSGPESDESKTKSFIYAPFQENPSRSNTAFREYYYLSLRRILIGGKP 307

Query: 361 VKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRATGVEAQSGL 420
           VKFPYKYLVPDS G GGAIIDSGSTFTFLDKPIFEA+A ELEKQLVKYPRA  +EA++GL
Sbjct: 308 VKFPYKYLVPDSTGKGGAIIDSGSTFTFLDKPIFEAIAGELEKQLVKYPRAKDIEAKTGL 367

Query: 421 RPCFDISKEEK-VEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMMTDEAGSGGGG 480
           RPCF+ISKEE+  EFPE+ LKFKGG KL+LPP NYL +VTD+ VVCLTMMT+ A   G G
Sbjct: 368 RPCFNISKEEESAEFPEVALKFKGGGKLSLPPENYLVMVTDANVVCLTMMTN-AEVVGVG 427

Query: 481 GGPAIIIGAFQQQNVLVEYDLAKERIGFRKQRCT 508
           GGPAII GAFQQQNVLVEYDLAK+RIGFRKQ+CT
Sbjct: 428 GGPAIIFGAFQQQNVLVEYDLAKQRIGFRKQKCT 460

[Close]

BLAST of Lag0012806 vs. TAIR 10
Match: AT3G52500.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 488.8 bits (1257), Expect = 5.3e-138
Identity = 244/464 (52.59%), Postives = 324/464 (69.83%), Query Frame = 0


Query: 61  FHFIFFLQL--PITLPLTTFPSLPLT--DPWQNINYLVSASLTRAHHLK----------- 120
           F F+ FL +   + LPL+ F     +  DP+ ++  L  +S+ RAH LK           
Sbjct: 7   FFFLIFLSVVSAVKLPLSPFSHSDQSPKDPYLSLRRLAESSIARAHKLKHGTSIKPDEDA 66

Query: 121 -SPRTNPNSSIEKVSLFSRTYGAYSISLSFGTPPQNFSFVFDTGSSLVWFPCTAGYLCSN 180
            S  T  ++++ K  L +++YG YS+SLSFGTP Q   FVFDTGSSLVW PCT+ YLCS 
Sbjct: 67  LSSTTTASATVVKSPLSAKSYGGYSVSLSFGTPSQTIPFVFDTGSSLVWLPCTSRYLCSG 126

Query: 181 CSFPNSDTKTIAKFIPKLSSSAKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPG 240
           C F   D   I +FIPK SSS+KI+GC++ KC +++GPN+  +CR C+P +R C   CP 
Sbjct: 127 CDFSGLDPTLIPRFIPKNSSSSKIIGCQSPKCQFLYGPNV--QCRGCDPNTRNCTVGCPP 186

Query: 241 YGIQYGSGATAGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRL 300
           Y +QYG G+TAG L++E LDFP+  V DF+VGCS++S   PAGIAGFGRGP SLPSQM L
Sbjct: 187 YILQYGLGSTAGVLITEKLDFPDLTVPDFVVGCSIISTRQPAGIAGFGRGPVSLPSQMNL 246

Query: 301 KRFSYCLVSRRFDDSPVSSPLVLDSGS-ESGQSKTNSLIYAPFRENPSGSNAAFREFYYL 360
           KRFS+CLVSRRFDD+ V++ L LD+GS  +  SKT  L Y PFR+NP+ SN AF E+YYL
Sbjct: 247 KRFSHCLVSRRFDDTNVTTDLDLDTGSGHNSGSKTPGLTYTPFRKNPNVSNKAFLEYYYL 306

Query: 361 SLRRILIVGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYP 420
           +LRRI +  K VK PYKYL P + G+GG+I+DSGSTFTF+++P+FE VAEE   Q+  Y 
Sbjct: 307 NLRRIYVGRKHVKIPYKYLAPGTNGDGGSIVDSGSTFTFMERPVFELVAEEFASQMSNYT 366

Query: 421 RATGVEAQSGLRPCFDISKEEKVEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMM 480
           R   +E ++GL PCF+IS +  V  PEL+ +FKGGAKL LP +NY   V ++  VCLT++
Sbjct: 367 REKDLEKETGLGPCFNISGKGDVTVPELIFEFKGGAKLELPLSNYFTFVGNTDTVCLTVV 426

Query: 481 TDEAGSGGGGGGPAIIIGAFQQQNVLVEYDLAKERIGFRKQRCT 508
           +D+  +  GG GPAII+G+FQQQN LVEYDL  +R GF K++C+
Sbjct: 427 SDKTVNPSGGTGPAIILGSFQQQNYLVEYDLENDRFGFAKKKCS 468

[Close]

BLAST of Lag0012806 vs. TAIR 10
Match: AT5G45120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 197.6 bits (501), Expect = 2.4e-50
Identity = 156/483 (32.30%), Postives = 223/483 (46.17%), Query Frame = 0


Query: 64  IFFLQLPITLPLTTFPSLPL---TDPWQNINYLVSASLTRAH-HLKSPRTNPNSSIEK-- 123
           + FL L ITL L T          +P  + +  +  +LT++   L +P++     I+K  
Sbjct: 7   VLFLFLLITLLLNTTNKTQARQHKNPSSSSSSFLVLTLTKSSVSLPTPKSQTQERIKKPL 66

Query: 124 ------VSLFSRTYGAYSISLSFGTPPQNFSFVFDTGSSLVWFPC-TAGYLCSNC-SFPN 183
                 +         Y I+L+ GTPPQ      DTGS L W PC    + C  C    N
Sbjct: 67  SSVDVVMEPLREVRDGYLITLNIGTPPQAVQVYLDTGSDLTWVPCGNLSFDCIECYDLKN 126

Query: 184 SDTKTIAKFIPKLSSSAKIVGCRNRKCAWI------FGPNLKSRCRNCNPKSRRCFDSCP 243
           +D K+ + F P  SS++    C +  C  I      F P   + C         C   CP
Sbjct: 127 NDLKSPSVFSPLHSSTSFRDSCASSFCVEIHSSDNPFDPCAVAGCSVSMLLKSTCVRPCP 186

Query: 244 GYGIQYGSGA-TAGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGRGPESLPSQM 303
            +   YG G   +G L  + L    + V  F  GC   +   P GIAGFGRG  SLPSQ+
Sbjct: 187 SFAYTYGEGGLISGILTRDILKARTRDVPRFSFGCVTSTYREPIGIAGFGRGLLSLPSQL 246

Query: 304 RL--KRFSYCLVSRRFDDSP-VSSPLVLDSGSESGQSKTNSLIYAPFRENPSGSNAAFRE 363
               K FS+C +  +F ++P +SSPL+L + + S  + T+SL + P    P   N+    
Sbjct: 247 GFLEKGFSHCFLPFKFVNNPNISSPLILGASALS-INLTDSLQFTPMLNTPMYPNS---- 306

Query: 364 FYYLSLRRILIVGK--PVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEK 423
            YY+ L  I I     P + P      DS GNGG ++DSG+T+T L +P +  +   L+ 
Sbjct: 307 -YYIGLESITIGTNITPTQVPLTLRQFDSQGNGGMLVDSGTTYTHLPEPFYSQLLTTLQ- 366

Query: 424 QLVKYPRATGVEAQSGLRPCF----------DISKEEKVEFPELVLKFKGGAKLALPPAN 483
             + YPRAT  E+++G   C+           +  +  + FP +   F   A L LP  N
Sbjct: 367 STITYPRATETESRTGFDLCYKVPCPNNNLTSLENDVMMIFPSITFHFLNNATLLLPQGN 426

Query: 484 ---YLALVTDSGVV-CLTMMTDEAGSGGGGGGPAIIIGAFQQQNVLVEYDLAKERIGFRK 507
               ++  +D  VV CL     E     G  GPA + G+FQQQNV V YDL KERIGF+ 
Sbjct: 427 SFYAMSAPSDGSVVQCLLFQNME----DGDYGPAGVFGSFQQQNVKVVYDLEKERIGFQA 478

[Close]

BLAST of Lag0012806 vs. TAIR 10
Match: AT4G16563.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 189.1 bits (479), Expect = 8.6e-48
Identity = 159/498 (31.93%), Postives = 230/498 (46.18%), Query Frame = 0


Query: 61  FHF-IFFLQLPITLPLTTFPSLPLTDPWQNINYLVSASLTRAHHLKSPRTNPNSSIEKVS 120
           FHF +  L  P+ L L+   SL  +    +  +L+ +S +R+   +  R +     +++S
Sbjct: 18  FHFSVSSLSTPLLLHLS--HSLSTSKHSSSPLHLLKSSSSRS-SARFRRHHHKQQQQQLS 77

Query: 121 LFSRTYGAYSISLSFGTPPQNFSFVFDTGSSLVWFPCTAGYLCSNCSFPNSDTKTIAKFI 180
           L   +   Y ISLS G+     S   DTGS LVWFPC   + C  C     ++K +    
Sbjct: 78  LPISSGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPCRP-FTCILC-----ESKPLPPSP 137

Query: 181 P-KLSSSAKIVGCRNRKCAWIFGPNLKS-------------RCRNCNPKSRRCFDSCPGY 240
           P  LSSSA  V C +  C+        S                +CN  S      CP +
Sbjct: 138 PSSLSSSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSY----PCPPF 197

Query: 241 GIQYGSGATAGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRL- 300
              YG G+    L S++L  P   V++F  GC+  ++  P G+AGFGRG  SLP+Q+ + 
Sbjct: 198 YYAYGDGSLVAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVH 257

Query: 301 -----KRFSYCLVSRRFDDSPV--SSPLVL-----------------DSGSESGQSKTNS 360
                  FSYCLVS  FD   V   SPL+L                 D G +  + K N 
Sbjct: 258 SPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDE-KKKKNE 317

Query: 361 LIYAPFRENPSGSNAAFREFYYLSLRRILIVGKPVKFPYKYLVPDSAGNGGAIIDSGSTF 420
            ++    ENP         FY +SL+ I I  + +  P      D  G GG ++DSG+TF
Sbjct: 318 FVFTEMLENPK-----HPYFYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTF 377

Query: 421 TFLDKPIFEAVAEELEKQLVK-YPRATGVEAQSGLRPCFDISKEEKVEFPELVLKFKGG- 480
           T L    + +V EE + ++ + + RA  VE  SG+ PC+ +++  KV  P LVL F G  
Sbjct: 378 TMLPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLNQTVKV--PALVLHFAGNR 437

Query: 481 AKLALPPANYLALVTDSG--------VVCLTMMT--DEAGSGGGGGGPAIIIGAFQQQNV 507
           + + LP  NY     D G        + CL +M   DE+   GG G    I+G +QQQ  
Sbjct: 438 SSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGA---ILGNYQQQGF 491

[Close]

BLAST of Lag0012806 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 168.3 bits (425), Expect = 1.6e-41
Identity = 140/427 (32.79%), Postives = 188/427 (44.03%), Query Frame = 0


Query: 88  QNINYLVSASLTRAHHLKSPRTNPNSSIEKVSLFSRTYGAYSISLSFGTPPQNFSFVFDT 147
           ++I  L + S  R    ++PRT    S   +S  S+  G Y + L  GTP  N   V DT
Sbjct: 95  KSITSLAAVSTGRNATKRTPRTAGGFSGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDT 154

Query: 148 GSSLVWFPCTAGYLCSNCSFPNSDTKTIAKFIPKLSSSAKIVGCRNRKCAWIFGPNLKSR 207
           GS +VW  C+    C N        +T A F PK S +   V C +R C       L   
Sbjct: 155 GSDVVWLQCSPCKACYN--------QTDAIFDPKKSKTFATVPCGSRLCR-----RLDDS 214

Query: 208 CRNCNPKSRRCFDSCPGYGIQYGSGA-TAGFLLSETLDFPEKRVTDFLVGCSVLSVHL-- 267
                 +S+ C      Y + YG G+ T G   +ETL F   RV    +GC   +  L  
Sbjct: 215 SECVTRRSKTCL-----YQVSYGDGSFTEGDFSTETLTFHGARVDHVPLGCGHDNEGLFV 274

Query: 268 -PAGIAGFGRGPESLPSQMRLK---RFSYCLVSRRFDDSPVSSPLVLDSGSESGQSKTNS 327
             AG+ G GRG  S PSQ + +   +FSYCLV R    S    P  +  G+ +   KT+ 
Sbjct: 275 GAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLVDRTSSGSSSKPPSTIVFGN-AAVPKTS- 334

Query: 328 LIYAPFRENPSGSNAAFREFYYLSLRRILIVGKPVK-FPYKYLVPDSAGNGGAIIDSGST 387
            ++ P   NP         FYYL L  I + G  V          D+ GNGG IIDSG++
Sbjct: 335 -VFTPLLTNPK-----LDTFYYLQLLGISVGGSRVPGVSESQFKLDATGNGGVIIDSGTS 394

Query: 388 FTFLDKPIFEAVAEELEKQLVKYPRATGVEAQSGLRPCFDISKEEKVEFPELVLKFKGGA 447
            T L +P + A+ +       K  RA    + S    CFD+S    V+ P +V  F GG 
Sbjct: 395 VTRLTQPAYVALRDAFRLGATKLKRA---PSYSLFDTCFDLSGMTTVKVPTVVFHF-GGG 454

Query: 448 KLALPPANYLALVTDSGVVCLTMMTDEAGSGGGGGGPAIIIGAFQQQNVLVEYDLAKERI 507
           +++LP +NYL  V   G  C            G  G   IIG  QQQ   V YDL   R+
Sbjct: 455 EVSLPASNYLIPVNTEGRFCFAF--------AGTMGSLSIIGNIQQQGFRVAYDLVGSRV 483

[Close]

BLAST of Lag0012806 vs. TAIR 10
Match: AT3G59080.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 159.5 bits (402), Expect = 7.3e-39
Identity = 122/397 (30.73%), Postives = 180/397 (45.34%), Query Frame = 0


Query: 126 GAYSISLSFGTPPQNFSFVFDTGSSLVWFPCTAGYLCSNCSFPNSDTKTIAKFIPKLSSS 185
           G Y + +  G+PP++FS + DTGS L W  C     C +C   N      A + PK S+S
Sbjct: 168 GEYFMDVLVGSPPKHFSLILDTGSDLNWIQCLP---CYDCFQQNG-----AFYDPKASAS 227

Query: 186 AKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPGYGIQYGSGATAGFLLSETL-- 245
            K + C +++C  +  P+    C++ N        SCP Y     S  T G    ET   
Sbjct: 228 YKNITCNDQRCNLVSSPDPPMPCKSDN-------QSCPYYYWYGDSSNTTGDFAVETFTV 287

Query: 246 -------DFPEKRVTDFLVGCSVLS---VHLPAGIAGFGRGPESLPSQMRL---KRFSYC 305
                        V + + GC   +    H  AG+ G GRGP S  SQ++      FSYC
Sbjct: 288 NLTTNGGSSELYNVENMMFGCGHWNRGLFHGAAGLLGLGRGPLSFSSQLQSLYGHSFSYC 347

Query: 306 LVSRRFDDSPVSSPLVLDSGSESGQSKTNSLIYAPFRENPSGSNAAFREFYYLSLRRILI 365
           LV R   D+ VSS L+   G +       +L +  F    +G       FYY+ ++ IL+
Sbjct: 348 LVDRN-SDTNVSSKLIF--GEDKDLLSHPNLNFTSF---VAGKENLVDTFYYVQIKSILV 407

Query: 366 VGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEEL-EKQLVKYPRATGVE 425
            G+ +  P +     S G GG IIDSG+T ++  +P +E +  ++ EK   KYP      
Sbjct: 408 AGEVLNIPEETWNISSDGAGGTIIDSGTTLSYFAEPAYEFIKNKIAEKAKGKYPVYRDFP 467

Query: 426 AQSGLRPCFDISKEEKVEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMMTDEAGS 485
               L PCF++S    V+ PEL + F  GA    P  N    + +  +VCL M+      
Sbjct: 468 I---LDPCFNVSGIHNVQLPELGIAFADGAVWNFPTENSFIWLNED-LVCLAML------ 527

Query: 486 GGGGGGPAIIIGAFQQQNVLVEYDLAKERIGFRKQRC 507
            G       IIG +QQQN  + YD  + R+G+   +C
Sbjct: 528 -GTPKSAFSIIGNYQQQNFHILYDTKRSRLGYAPTKC 532

The following BLAST results are available for this feature:

BLAST of Lag0012806 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022979057.1	4.1e-217	84.74	probable aspartyl protease At4g16563 [Cucurbita maxima]	[more]
XP_023543736.1	9.1e-217	84.90	probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo]	[more]
XP_022925946.1	2.9e-215	84.21	probable aspartyl protease At4g16563 [Cucurbita moschata] >KAG6604319.1 Aspartic...	[more]
KAG7034471.1	6.5e-215	84.21	Aspartic proteinase nepenthesin-1, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_038881211.1	8.2e-210	82.28	probable aspartyl protease At4g16563 [Benincasa hispida]	[more]

BLAST of Lag0012806 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q940R4	1.2e-46	31.93	Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g1656...	[more]
Q766C3	6.2e-35	30.51	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q9LNJ3	8.2e-35	32.27	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q9LS40	6.9e-34	30.46	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q766C2	4.9e-32	31.46	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]

BLAST of Lag0012806 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IMR7	2.0e-217	84.74	probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111478813...	[more]
A0A6J1EDJ0	1.4e-215	84.21	probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC1114332...	[more]
A0A0A0KHK2	5.4e-207	79.56	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G45447...	[more]
A0A5A7SGF9	7.3e-204	78.19	Aspartic proteinase nepenthesin-2-like OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CHV2	7.3e-204	78.19	aspartic proteinase nepenthesin-2-like OS=Cucumis melo OX=3656 GN=LOC103500932 P...	[more]

BLAST of Lag0012806 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52500.1	5.3e-138	52.59	Eukaryotic aspartyl protease family protein	[more]
AT5G45120.1	2.4e-50	32.30	Eukaryotic aspartyl protease family protein	[more]
AT4G16563.1	8.6e-48	31.93	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	1.6e-41	32.79	Eukaryotic aspartyl protease family protein	[more]
AT3G59080.1	7.3e-39	30.73	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 134..154 score: 54.29 coord: 372..383 score: 36.31
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 311..507 e-value: 3.5E-50 score: 172.2
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 102..310 e-value: 1.0E-31 score: 112.4
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 124..506
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 128..298 e-value: 3.0E-28 score: 99.2
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 340..502 e-value: 1.5E-35 score: 122.3
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 60..506
None	No IPR available	PANTHER	PTHR47967:SF36	BNACNNG47670D PROTEIN	coord: 60..506
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 143..154
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 128..502 score: 34.939892
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 127..506 e-value: 4.17758E-72 score: 228.301

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0012806.1	Lag0012806.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity